KR20150078948A - Cosmetic composition for improving skin inflammation or reducing skin irritation - Google Patents

Abstract

The present invention relates to a cosmetic composition including a mixture extract of Viscum album var. coloratum extract, skin of pine trees extract and Orostachys japonicus extract as active ingredients, thereby being used for improving skin inflammation or reducing skin irritation.

Description

[0001] The present invention relates to a cosmetic composition for improving skin inflammation or reducing skin irritation,

The present invention relates to a cosmetic composition effective for improving skin irritation or alleviating skin irritation.

The surface of the skin of the human being is naturally well mixed with sebum secreted from sebaceous glands and sweat glands and covers the stratum corneum, so that it remains smooth, shiny and moist. When the moisture content of the skin stratum corneum is about 20%, the skin becomes ideal and beautiful. When the moisture content of the skin stratum corneum is lowered to 10% or less, keratinous substances are removed from the skin and foreign substances are easily penetrated. And allergies are likely to be induced. Especially, as the age increases, the metabolism of the skin is not smooth, so various inflammation and allergy become heavy. In addition, people are becoming more susceptible to external stimuli such as dry indoor environment, pollution, ultraviolet rays, wind, misused cosmetics and detergents. Skin that is sensitized by external stimuli may show a merely visible response such as erythema or may show a very severe and unpleasant inflammatory reaction such as itching and stinging. Therefore, it is somewhat burdensome and continuous to use a popular skin cosmetic composition As shown in FIG.

Although cosmetics are products used for protecting skin and cleansing the hair, a cosmetic composition is specifically used, and components different from the original skin protection purpose are used as indispensable substances for product formation. These include, for example, solubilizers, emulsifiers, preservatives, fragrances, sunscreens, and other additives used to impart other benefits and effects. Generally, these ingredients are used to treat skin irritations, allergies, rashes, Is known to be the major causative agent.

Inflammation is a series of defenses aimed at minimizing the response of a cell or tissue to any cause and restoring the damaged area to its original state. The inflammation is caused by physical factors such as trauma, burns, frostbite, Chemical factors such as acids and bases, immunological factors due to antibody reactions, and other factors such as blood vessels and hormone imbalances. Histamine is the most well-known substance that mediates itching and pain. It has been found that there is no specific receptor to detect itching and it is presumed to be caused by an unspecified autonomic nerve ending in the gap between the epidermal cells or the epidermis and the epidermis like local pain. Substances that mediate itching and pain are released primarily from mast cells and are released by cross-linking FcεRIα receptors. In addition, histamine can be liberated from skin mast cells by inflammatory mediators such as substrate P, hepatocyte factor, vasoactive growth peptide, and the Fc? RI? Receptor.

To date, the chemical substances that can be used to relieve irritation and inflammation, such as erythema and edema, include nonsteroidal substances such as flufenamic acid, ibuprofen, benzydamine, indomethacin, indomethacin, and prednisolone, dexamethasone, etc. (Kim Chang-jong, pathology physiology, Hallym Company, p61-69, 1988), and other allantoin, azrene, ε-aminocaproic acid , Hydrocortisone (v), licorice acid and its derivatives (β-glycyrrhetinic acid, glycyrrhetinic acid derivative) are also known to be effective for anti-inflammation (Kwangjungbu, Shin,

Since the cause of skin adverse effects in cosmetics is always present, various studies have been conducted to solve this problem. Indomethacin, which is used as an anti-inflammatory agent, is a substance which can not be used in cosmetics. Hydrocotizine has a limited use amount. The derivative is difficult to stabilize or has a poor solubility and it is difficult to achieve a practical effect due to the limitation of the concentration when actual commercialization is made. Thus far, known anti-inflammatory agents have problems in terms of skin safety and stability in mixing cosmetics Its use is limited.

The present inventors have intensively studied to develop a cosmetic composition capable of reducing skin irritation and inflammation caused by various stress sources more efficiently and reducing skin side effects caused by use of cosmetics and the like, The present invention has been accomplished by confirming a combination of natural plant extracts having particularly excellent synergistic effect.

Kim, Chang - Jong, Pathophysiology, Hallym University, p61-69, 1988 Kwangjungbu, New Cosmetics, Namsan-dong, p162, 1993

It is an object of the present invention to provide a cosmetic composition containing a natural plant extract combination exhibiting synergistic effects in improving skin inflammation or alleviating skin irritation.

In order to achieve the above object, the present invention provides a cosmetic composition comprising, as an active ingredient, a mixed extract of mistletoe extract, pine bark extract, and rosemary extract.

Hereinafter, the present invention will be described in more detail.

Viscum album var. Is a parasitic parasitic plant in mulberry trees, oak trees, Japanese cedar trees, chestnut trees, chestnut trees and birch trees from 50 to 1100m above sea level in China, Taiwan, Japan and Korea. It is an evergreen parasitic shrub. It grows round in a nest shape and it reaches 1m in diameter. The branch is split into an upper part, round, yellowish green, without hair, and between the nodes is 3 ~ 6cm. Leaves are larvae and lanceolate, 3 ~ 6cm in length and 6 ~ 12cm in width. They are obtuse and umbilical. They become narrower and narrower as they go down. They are dark green with thick petioles, (潤 彩) there is no. Flowers bloom in January to March, flowers are yellow, and the majestic flowers are male and female. There are no flower buds, and small bracts are cup-shaped. It is like a bell, it is divided into four, and the head of the stigma is not. The fruit is matured from December to the following January, and the nucleus is round and pale yellow with a diameter of about 6mm, and leaves of flower lacquer and stigma are left at the end. It is called red mistletoe (for. Rubroaurantiacum OHWI) that fruit is ripened in red color. It is used for ornamental and medicinal purposes. It is used for the treatment of leaves and stalks in one or both of the private and public sectors, and plants (plant bodies) are used for the treatment of diseases such as tuberculosis, lumbago, hypertension, hypothermia, postpartum, frostbite and arteriosclerosis. Write it as a medicinal product.

The pine bark extract contains a large amount of natural polyphenols. The polyphenols are widely distributed in the leaves, stems, roots and seeds of plants. Especially, the high quality natural polyphenols extracted from the pine bark have strong anti- Inflammatory and antimicrobial activity as well as antioxidant, antiviral, immunity enhancement, improvement of asthma, promotion of blood circulation, improvement of arteriosclerosis, prevention of blood clotting, Pharmaceuticals, feed additives and so on. In particular, studies on pycnogenol, a bark extract of French sea shell, have shown that the extract of pine bark has anti-inflammatory effects (M. Stenfanescu et al., Phytotherapy Research 15: 698-704; 2001) Phytother Res 16: 567-571, 2002), skin elasticity enhancement effect (D. Segger and F. Schonlau, J. Biol. Med. 36: 811-822, 2004). Dermatol. Treatment, 15: 1-5, 2004), edema improvement (Arcangeli, Fitoterapia 71: 236-244, 2000), Kohama & Suzuki, Eur Bull Drug Res, 7: 30-32 Diabetes Care 27 (3): 839, 2004), a blood pressure lowering effect (Hosseini et al., et al., Nutrition Res 21: 1251-1260, 2001), and the domestic pine bark extract has a similar physiological activity. It has been shown by the study of muscle.

Orostachys japonicus is a perennial herbaceous plant of Crassulaceae, which is also called Syojong (Ishatsu), Oksong (laver), Leafy lawn (grasshopper) And meat quality. (Park HJ, Lim SC, LEE MS, Kwon SJ, Park HJ, Kim JH, Park JH, et al. Triterpene and steroids from Orostachys japonicus. Kor J Pharmacogn, 1994, 25 (1): 20-24). There are vasoconstrictors, respiratory stimulants, intestinal intensification, hypotension, diuretic and antipyretic effects in the pharmacological effects of warsong (Yeomlim, 1990, 600 ~ 601). This study was conducted to investigate the effect of cisplatin on the inhibition of renal toxin and the antitumor effect of cisplatin on the anticancer activity of Pseudomonas aeruginosa, 282 ~ 303), and the effect of Wassong on the natural killer cells of rats (Yang, Seo Hyun, Duk-Ho Kim, Woo-Heung Jung, etc. Effects of Yeongji, Sanzago, , 1989, 10: 103-114) and cervical cancer have been reported. Until now, the components of the salmon that have been isolated and reported include triterpenoids such as friedelin, epi-friedelanol, glutinone, glutinol, Sterol-based substances such as β-sitosterol and campesterol, fatty acid esters and flavonoids such as kaempferol and quercetin, 4-hydroxybenzoic acid, 3,4 Aromatic hydrocarbons such as dihydroxybenzoic acid and gallic acid (Park HJ et al. Kor J Pharmacogn, 1991, 22 (2): 78-84; Park HJ et al. Park JG et al., Nat Prod Sci, 2000, 6 (3): 117 < RTI ID = 0.0 > 121).

In the cosmetic composition of the present invention, each of the three primary drug components as described above is harmonized with each other and complementary to each other, so that each component can be used alone or in combination with only a part of components Skin inflammation improvement or skin irritation mitigation effect.

In addition, the cosmetic composition of the present invention may contain one or more other active ingredients that exhibit anti-inflammatory and anti-irritating effects, if necessary.

The mistletoe extract, the pine bark extract, and the rock extract of the present invention contained in the cosmetic composition of the present invention can be dissolved in water, an alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof , Functional alcohols, functional glycerin, functional ethylene glycols, water-containing propylene glycols, and functional butylene glycols). Preferably a solvent selected from water, alcohols having 1 to 4 carbon atoms, and mixtures thereof. Here, the alcohol having 1 to 4 carbon atoms refers to methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol, tert-butanol and the like. The functional alcohol may contain from 0.3 to 90 (wt / wt)% alcohol.

More preferably 30 to 90 (wt / wt)%, still more preferably 50 to 80 (wt / wt)% and most preferably 70 (wt / wt)% Functional ethanol can be used.

The mixed extract of the mistletoe extract, pine bark extract and rosemary extract of the present invention can be prepared by a known extraction method known to the ordinarily skilled artisan. The extraction temperature may vary depending on the extraction solvent, but is preferably 5 to 100 캜.

For example, the dried product of mistletoe, pine bark and rocksol is sieved and an extraction solvent such as water, 1- to 4-fold alcohols or alcohols having a carbon number of 1 to 50, Deg.] C for 3 to 20 days, extracting the active ingredient, and concentrating the extraction solvent with a vacuum concentrator. Alternatively, the dried product of the mistletoe, the pine bark and the rocksol of the present invention may be pulverized and mixed, and water of 1 to 30 times the mixed dry weight thereof, an alcohol of 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene At least one solvent selected from the group consisting of glycols, butylene glycols and mixtures thereof is used, followed by heat extraction at 30 to 100 DEG C for 3 to 48 hours with an extractor equipped with a cooling condenser to prevent evaporation of the solvent Immersion at 5 to 30 DEG C for 1 to 5 days to extract an active ingredient, and concentrating the extracting solvent with a vacuum concentrator. Further, it can be produced by using an ultrasonic extraction method known in the above-mentioned extraction solvent. Alternatively, the extract can be prepared by extracting the mistletoe, pine bark and rosaceous extract according to the above-mentioned extraction solvent and extraction conditions, respectively, and mixing them.

The mixed extract of mistletoe extract, pine bark extract, and rock extract of the present invention may be a concentrate obtained by concentrating the extract obtained by extracting the extract with reduced pressure, a lyophilized powder obtained by concentrating the concentrate, or a powder obtained by spray drying and concentrating the concentrate.

In the cosmetic composition of the present invention, the mixed extract of the mistletoe extract, pine bark extract, and rosemary extract of the present invention may be added to the cosmetic in an amount of 0.001 to 10.0% by weight based on the total weight of the cosmetic composition, more preferably 0.001 to 10.0% May be added to the cosmetic in an amount of 5.0% by weight. If the amount is lower than the above range, the effect of anti-inflammation or anti-irritation of the cosmetic composition may be insignificant, and if it is higher than the above range, the cosmetic composition may have browning or odor.

In the cosmetic composition of the present invention, the mistletoe extract, the pine bark extract, and the rosso extract may preferably be contained in a weight ratio of 1: 1-3: 1-3. If the weight ratio is out of the range, stability and efficacy of the cosmetic composition may be lowered, so that the above range is suitable.

The mixed extract of mistletoe, pine bark and rosaceol of the present invention showed a very excellent free radical scavenging ability (antioxidative effect) (see Experimental Example 1 and Table 3), effectively relieving skin irritation caused by a typical skin cell stimulating agent such as lactic acid (See Experimental Example 3 and Table 5). In particular, it exhibited superior skin irritation-improving effects far superior to the typical anti-inflammatory substance indomethacin (see Experimental Example 4 and Table 6). The mixed extracts of mistletoe, pine bark and rocksol of the present invention exhibited remarkably superior synergistic effects in terms of antioxidative effect, anti-inflammatory effect and skin irritation mitigating effect, compared with the single extracts of each of mistletoe, pine bark and rock salt , 5 and 6).

The mixed extract of mistletoe, pine bark and rosacea of the present invention exhibits excellent anti-inflammatory effect and skin irritation-mitigating effect as described above, and thus can be effectively used as a cosmetic for improving skin inflammation or alleviating skin irritation.

The cosmetic composition of the present invention is not particularly limited in its formulation and may be formulated in the form of softening agent, nutritional lotion, nutritional lotion, nutritional cream, massage cream, nutritional serum, essence, pack, . In addition, in the cosmetic composition of each formulation, other components than the above-mentioned mixed extract of mistletoe, pine bark, and rockfish can be arbitrarily selected and formulated according to the formulation or purpose of use of other cosmetics. For example, it may include colorants (coloring matters), flavoring agents (flavoring agents), suspending agents, emulsifying agents, dissolution aids, stabilizers, isotonic agents, pH adjusting agents, viscosity adjusting agents, solvents, preservatives and the like which are conventionally used in cosmetic compositions .

The cosmetic composition comprising the mixed extract of the mistletoe extract, pine bark extract, and rosemary extract of the present invention as an active ingredient exhibits synergistic effects for improving skin irritation or alleviating skin irritation and is effective for improving skin inflammation or alleviating skin irritation Can be effectively used.

Hereinafter, the constitution and effects of the present invention will be described in detail with reference to examples, comparative examples and formulation examples, but the present invention is not limited thereto.

< Example  1> Powder production of mixed extract of mistletoe, pine bark, and rocksol

Pine bark, and rosso were purchased from Kyungdong market. The dried mist, pine bark, and rosin were added to 2 g of dried distilled water. The extracts were boiled at 80 ℃ for 2 hrs. The extract was filtered through a 300-mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered twice with a filter paper of Edbentek No. 5 and Wattman GF / C 150 mm filter paper. Then, the mixture was concentrated using a vacuum concentrator at a temperature of 40 to 50 ° C., and dried using a drier (model name: B-290, manufactured by BUCHI) under the following conditions to obtain 11 g of the mixed extract powder of the title product (Krussmann, 1982) .

- Inlet temperature 180 ° C,

- Aspirator Efficiency 100% (35 cm3 / hour),

- Pump efficiency 25% (7.5 ml / min),

- Nozzle Cleaner 4,

- Rotameter 30 mm (357 Liter / hour)

< Example  2 to 5> Preparation of powders of mistletoe, pine bark and rocksol mixed extract according to the extraction solvent

Mistletoe, pine bark, and rocksol were extracted in the same manner as in Example 1, except that the solvent described in Table 1 was used as the extraction solvent to prepare the mixed extract powder of the present invention. The results are shown in Table 1 below.

division Extractor Yield (g) Example 2 30% functional ethanol 13 Example 3 50% functional ethanol 14 Example 4 70% functional ethanol 17 Example 5 80% functional ethanol 16

< Comparative Example  1 to 3> Preparation of powders of individual extracts of each of the mistletoe, pine bark,

The extracts of the mistletoe, the pine bark, and the rocksol were extracted and filtered in the same manner as described in Example 1 to prepare a single extract powder. The results are shown in Table 2 below.

division Powder (g) Comparative Example 1 (Moultry extract alone) 13 Comparative Example 2 (Pine bark solely extract) 12 Comparative Example 3 (Porphyromycin alone extract) 7

< Experimental Example  1> Antioxidant effect test

The free radical scavenging activity of the extracts of Examples 1 to 5 and Comparative Examples 1 to 3 was measured to determine the currency antioxidative effect. 0.9 mL of 4.1 × 10 ^-5 M DPPH solution was added to 0.1 mL of each extract, followed by reaction at room temperature for 30 minutes to measure the absorbance at 517 nm. As a control, L-ascorbic acid (100 μg / ml) was used, and the radical scavenging activity of each sample was calculated by the following equation and shown in Table 3.

[Formula 1]

division Radical scavenging ability (%) Example 1 53.10 Example 2 63.30 Example 3 88.90 Example 4 98.50 Example 5 94.70 Comparative Example 1 35.50 Comparative Example 2 36.10 Comparative Example 3 29.60

As shown in Table 3, most of the mixed extracts of mistletoe, pine bark and rocksol of the present invention exhibited high erasure rates, and the mixed extract of Example 4 containing 70% ethanol as a solvent showed the highest erasure rate Respectively. In particular, the mixed extracts of Examples 4 and 5 obtained by extracting with 70% and 80% ethanol showed higher erosion rates than L-ascorbic acid (100 μg / ml; 91.10% eradication rate)

< Experimental Example  2> Cytotoxicity and proliferation experiments

The cytotoxicity and proliferation experiments were carried out in the following manner. The cultured human normal fibroblasts were inoculated into each well of a 96-well microplate to a concentration of 1 x 10 ⁴ cells and cultured in DMEM medium at 37 ° C for 24 hours. Subsequently, the extracts of Examples 1 to 5 and Comparative Examples 1 to 3 were replaced with DMEM (Sigma, USA) medium containing 2.5% of serum at final concentrations of 0.1, 0.5, 1.0, 2.0 and 5.0% The control group, which was replaced with serum-free DMEM medium, was further cultured for 24 hours. After incubation, MTT (Sigma, USA) solution (3 mg / ml) was added to compare the cell viability and the cell viability was measured at 570 nm using an ELISA reader (Molecular Devices, USA) The cells were cultured under the same conditions as above, and the cell proliferation rate of the test sample was calculated by the following equation with the cell proliferation of the control group as 100%.

[Formula 2]

Cell proliferation (%) Control group 0.1% 0.5% 1.0% 2.0% 5.0% Example 1 100 101.6 101.0 101.8 100.4 98.6 Example 2 100 102.1 101.3 99.8 99.7 100.1 Example 3 100 103.2 101.4 101.0 100.7 100.5 Example 4 100 99.9 100.1 100.0 102.5 99.6 Example 5 100 102.1 101.4 100.9 101.1 100.8 Comparative Example 1 100 101.2 101.6 100.2 100.8 97.8 Comparative Example 2 100 102.9 101.2 100.1 99.5 100.8 Comparative Example 3 100 101.3 101.8 100.5 100.4 100.6

As shown in Table 4, when the cell proliferation at the optimal cell growth conditions is taken as 100%, the mixed extracts of the mistletoe, pine bark and rock bottom of the present invention are all cell non-toxic considering the measurement error range, The effect was confirmed.

< Experimental Example  3> Effect of lactic acid on mitigation of cell stimulation

The skin irritation mitigating effect of the mixed extract of the present invention was confirmed. Specifically, it was confirmed through an in vitro experiment using a representative skin irritant substance, lactic acid. Human fibroblasts (Korean Cell Line Bank) were cultured in T-75 flasks (Falcon, USA) until they grew to about 80%. The cultured cells were again transferred to each well of a 96-well plate (Falcon, USA) at 3 × 10 ⁴ cells / well and cultured for 24 hours. After culturing, cells were completely adhered to each other through a microscope to confirm whether they grew well or not. Then, experiments were conducted using lactic acid, a skin cell stimulating agent. At this time, lactic acid was used at a concentration of 0.2% to kill all the skin cells. That is, 200 쨉 l of DMEM (Sigma, USA) medium containing 0.2% lactic acid was added to each well of a 96-well plate, and the mixed extract of the present invention of Examples 1 to 5 and Comparative Examples 1 to 3 The sole extract was added at a controlled concentration of 100, 200, 500 and 1000 μg / ml. At this time, as a control group, no lactic acid and all of the examples were added, and a lactic acid supplemented group was used as a comparative group. MTT ([3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was added to each well in an amount of 10 μl to compare the survival rate of the cells after 12 hours . After 4 hours had elapsed from the addition of the MTT reagent, the absorbance at 570 nm was measured using an ELISA reader. Cell viability was calculated according to the following equation using the measured absorbance values and is shown in Table 5. The cell survival rate (%) is an index showing the mitigation effect of skin cell stimulation, and the higher the cell survival rate, the higher the mitigation effect on skin cell stimulation.

[Formula 3]

Cell survival rate (%) = [(absorbance of control - absorbance of test substance) / absorbance of control] × 100

Cell viability (%) 100 mu g / ml 200 [mu] g / ml 500 [mu] g / ml 1000 mu g / ml Example 1 38.3 46.7 57.2 84.2 Example 2 41.7 47.1 60.5 81.8 Example 3 43.4 50.6 64.9 87.1 Example 4 49.0 56.1 72.6 94.2 Example 5 47.8 54.7 69.8 92.6 Comparative Example 1 31.2 42.4 50.6 66.7 Comparative Example 2 30.2 41.5 49.8 65.8 Comparative Example 3 38.7 46.3 56.4 69.9 Control group 100 100 100 100 Comparative group 0 0 0 0

As shown in Table 5, the mixed extracts of mistletoe, pine bark, and rosacea according to the present invention had a significantly higher cell survival rate depending on the treatment concentration than the single extract treatment group or the comparative group of the comparative example.

< Experimental Example  4> Anti-inflammatory experiment

The above extract was suspended in an animal cell culture medium containing 10% FBS without phenol red, and 5 × 10 ⁵ cells / well of each of the 24 well plates were placed in 37 ° C. and 5% CO 2 ₂ And cultured under the culture conditions for 24 hours. RAW 264.7 cells were treated with lipopolysaccharide and the above example or comparative example extract or dimethylsulfoxide at the same time for 20 hours and reacted with 180 쨉 l of Grise's Reagent to measure the absorbance at 540 nm.

The extracts of the above Examples and Comparative Examples were dissolved in 1 ml of 95% ethanol and subjected to the anti-inflammatory test according to the above-described method at a concentration of 1 / / ㎖, 10 / / ㎖, 100 / / ㎖ and 1000 / / Respectively. The anti-inflammatory effect was calculated by the following equation (4), and the results are shown in Table 6 below.

[Formula 4]

Inhibition rate (%) 1 mu g / ml 10 mu g / ml 100 mu g / ml 1000 mu g / ml Example 1 2.27 8.24 40.17 71.16 Example 2 2.65 8.96 40.47 70.15 Example 3 3.05 9.18 45.94 74.13 Example 4 3.68 9.85 47.26 76.91 Example 5 3.47 9.54 46.92 75.96 Comparative Example 1 1.43 7.87 25.36 41.62 Comparative Example 2 1.62 7.96 26.74 42.33 Comparative Example 3 1.11 7.51 22.84 39.82 Control group 0.21 5.35 19.32 24.65

As shown in Table 6, it was confirmed that the mixed extract of the present invention had a superior anti-inflammatory effect than indomethacin used as a single extract or a control group.

< Formulation Example  1> Mistletoe, pine bark, Mixed extract of rosacea  Manufacture of used lotion

1 kg of lotion (skin lotion) containing the mixed extract of mistletoe, pine bark and rosacea (Example 4) was prepared by the following production method in the content of [Table 7] below.

number Raw material Content (% by weight) A B C One Mixed extract of mistletoe, pine bark,
(Example 4) 1.0 5.0 10.0 2 glycerin 3.0 3.0 3.0 3 Butylene glycol 2.0 2.0 2.0 4 Propylene glycol 2.0 2.0 2.0 5 Polyoxyethylene hardened castor oil 1.0 1.0 1.0 6 ethanol 10.0 10.0 10.0 7 Triethanolamine 0.1 0.1 0.1 8 antiseptic a very small amount a very small amount a very small amount 9 Pigment a very small amount a very small amount a very small amount 10 Spices a very small amount a very small amount a very small amount 11 Purified water Balance Balance Balance

In Table 7, the substances 2, 3, 4, and 8 were sequentially added to a certain amount of the substance 11, dissolved in the reaction vessel, and the substance 5 was dissolved by heating to about 60 ° C. Thereafter, the substance No. 10 was added to the reaction vessel and dissolved, and then the remaining substance No. 11 was added thereto. Finally, the materials 1, 6, 7, and 9 were added, stirred sufficiently, and aged at 25 ° C for 3 days to prepare the title lotion.

< Formulation Example  2> Nutritional lotion production using mixed extract of mistletoe, pine bark and ginseng

1 kg of nutritional lotion containing mixed extract of mistletoe, pine bark and rosacea (Example 4) was prepared by the following production method in the content of [Table 8] below.

number Raw material Content (% by weight) A B C One Mixed extract of mistletoe, pine bark,
(Example 4) 1.0 5.0 10.0 2 Wax 1.0 1.0 1.0 3 Polysorbate 60 1.5 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 0.5 5 Liquid paraffin 10.0 10.0 10.0 6 Sorbitan stearate 1.0 1.0 1.0 7 Pro-type glycerin monostearate 0.5 0.5 0.5 8 Stearic acid 1.5 1.5 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 1.0 1.0 10 Propylene glycol 3.0 3.0 3.0 11 Carboxy polymer 0.1 0.1 0.1 12 Triethanolamine 0.2 0.2 0.2 13 antiseptic a very small amount a very small amount a very small amount 14 Pigment a very small amount a very small amount a very small amount 15 Spices a very small amount a very small amount a very small amount 16 Purified water Balance Balance Balance

In Table 8, the materials 10, 11, 13, and 16 were heated to 80 to 85 ° C while mixing and stirring, and then charged into the production section. Then, the emulsifier was activated to produce 2,3,3,4,5,6,7,8, 9, and 12 were heated and dissolved at a temperature of 80 to 85 ° C, followed by emulsification. After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and then the substance 15 is added. After cooling to 45 ° C, the substance 14 is added and the substance 1 is added at 35 ° C. For 3 days to prepare the nutritional lotion of the title.

< Formulation Example  3> Nutritional essence production using mixed extract of mistletoe, pine bark and ginseng

1 kg of the nutritional essence containing the mixed extract of mistletoe, pine bark and rosacea (Example 4) was prepared by the following production method with the content of [Table 9] below.

number Raw material Content (% by weight) A B C One Mixed extract of mistletoe, pine bark,
(Example 4) 1.0 5.0 10.0 2 Xanthan gum 0.02 0.02 0.02 3 Butylene glycol 10.0 10.0 10.0 4 Soybean phospholipid 10.0 10.0 10.0 5 Hydroxyethyl cellulose 0.1 0.1 0.1 6 Sodium hyaluronate 5.0 5.0 5.0 7 Purified water Balance Balance Balance

In Table 9, materials 2, 3 and 5 were heated to between 40 and 45 ° C while mixing and stirring. Then, the mixture was put into a manufacturing part, and then an emulsifying agent was allowed to react. Then, materials 4, 6 and 7 were added to the manufacturing part and emulsified. After the emulsification was completed, the mixture was cooled to 35 ° C with stirring using an agitator, the material 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare the titled nutritional serum.

< Formulation Example  4> Manufacture of nutritional cream with mixed extract of mistletoe, pine bark and ginseng

1 kg of a nutritive cream containing a mixed extract of mistletoe, pine bark and rosacea (Example 4) was prepared using the following preparation method in the content of [Table 10] below.

number Raw material Content (% by weight) A B C One Mixed extract of mistletoe, pine bark,
(Example 4) 1.0 5.0 10.0 2 Sitosterol 1.7 1.7 1.7 3  Polyethylene glycol 1.5 1.5 1.5 4  Ceramide 0.7 0.7 0.7 5 Stearic acid 1.2 1.2 1.2 6 cholesterol 1.5 1.5 1.5 7 Dicetyl phosphate 0.4 0.4 0.4 8 Concentrated glycerin 5.0 5.0 5.0 9 Macadamia oil 15.0 15.0 15.0 10 Carboxyvinyl polymer 0.2 0.2 0.2 11 Xanthan gum 0.2 0.2 0.2 12 antiseptic a very small amount a very small amount a very small amount 13 Spices a very small amount a very small amount a very small amount 14 Purified water Balance Balance Balance

In Table 10, the materials 3, 8, 10, and 11 were heated to 80 to 85 ° C while mixing and stirring. The material was heated to dissolve at 80 to 85 캜 and emulsified. After the emulsification was completed, the mixture was cooled to 50 ° C with stirring using an agitator, and then the substance No. 13 was added, cooled to 45 ° C, and the substance No. 12 was added thereto. For 3 days to prepare nutritional cream of the title.

< Experimental Example  5> Confirm formulation stability

(25 ° C), 4 ° C and 45 ° C for the formulation of lotion, nutrition lotion and nutritional essence prepared in Formulation Examples 1 to 4 containing the mixed extract of mistletoe, pine bark and rock salt of the present invention The stability of these formulations was confirmed by observing discoloration and detachment with the naked eye and observing the cleavage by olfactory after storage for 12 weeks in an opaque glass container in a room, a refrigerator and a thermostatic water bath. The results are shown in [Table 11].

Temperature
Condition Stability check (discoloration, separation and separation) - Formulation example 1-A 1-B 1-C 2-A 2-B 2-C 3-A 3-B 3-C 4-A 4-B 4-C Room temperature 0 0 0 0 0 0 0 0 0 0 0 0 4 ℃ 0 0 0 0 0 0 0 0 0 0 0 0 45 ° C 0 0 0 0 0 0 0 0 0 0 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

As shown in Table 11, Formulation Examples 1 to 4 were stable at all the contents at room temperature, 4 占 폚 and 45 占 폚 under all temperature conditions without discoloration, separation, and separation phenomenon.

Claims (7)

A cosmetic composition comprising a mixed extract of mistletoe extract, pine bark extract and rosemary extract as an active ingredient. The cosmetic composition according to claim 1, wherein the extract is extracted using a solvent selected from water, alcohols having 1 to 4 carbon atoms, and mixtures thereof. The cosmetic composition according to claim 1, wherein the extract comprises 0.001 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin inflammation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for alleviating skin irritation. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises mistletoe extract, pine bark extract, and rosemary extract in a weight ratio of 1: 1-3: 1-3. The cosmetic composition according to claim 1, wherein the cosmetic composition is a formulation of a softening agent, a nutritional lotion, a nutritional lotion, a nutritional cream, a massage cream, a nutritional serum, an essence or a pack.