KR102470309B1 - Functional and hypoallergenic vegan cosmetic composition containing organic vegetable extract - Google Patents

Abstract

The present invention relates to a functional and hypoallergenic vegan cosmetic composition containing organic vegetable extracts, and a manufacturing method thereof. More specifically, the present invention relates to: a vegan cosmetic composition which contains only organic vegetable extracts as active ingredients, and thus does not irritate the skin while having improved functions such as skin moisturizing, skin whitening, and antioxidation; and a manufacturing method of the vegan cosmetic composition.

Description

Functional and hypoallergenic vegan cosmetic composition containing organic vegetable extract and its manufacturing method {FUNCTIONAL AND HYPOALLERGENIC VEGAN COSMETIC COMPOSITION CONTAINING ORGANIC VEGETABLE EXTRACT}

The present invention relates to a functional and hypoallergenic vegan cosmetic composition containing an organic vegetable extract and a manufacturing method thereof, and specifically, to include only an organic vegetable extract as an active ingredient to improve functionality such as moisturizing, whitening, and antioxidant, while irritating the skin. It relates to a vegan cosmetic composition without this and a manufacturing method thereof.

In general, as the primary barrier of the human body, the skin protects internal organs from external environmental stimuli such as temperature and humidity changes, ultraviolet rays, and pollutants, and plays an important role in maintaining body homeostasis such as body temperature control.

As the industry develops, environmental pollution becomes serious, and interest in skin oxidation and aging prevention, whitening, etc. is growing. In particular, human skin constantly undergoes changes, and the most representative changes are deterioration of skin function and visual beauty due to oxidation and aging.

Lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized and skin cells and tissues may be destroyed, resulting in aging of the skin. In particular, due to protein oxidation, collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are connective tissues of the skin, are cut, and excessive inflammatory reactions may occur. Induction of cancer may be promoted.

In addition, in general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, and becomes about 10-30% in the keratinocyte layer. The water supplied from the dermal layer moves to the top of the stratum corneum mainly by passive diffusion and is finally discharged to the outside. This is referred to as transepidermal water loss, and it is known that this transepidermal water loss is maintained at an appropriate level by sebum components and epidermal lipids, which are lipid film components of the stratum corneum layer. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes dry and skin aging occurs. Therefore, it is important to properly maintain the moisture content of the stratum corneum of the skin.

Conventionally, ascorbic acid (Japanese Patent Laid-open Hei 4-9320), hydroquinone (Japanese Patent Laid-Open No. 6-192062), kojic acid (Japanese Patent Laid-Open No. 56-7710), and arbutin (Japanese Patent Laid-Open No. 4-93150) inhibit tyrosinase. It showed activity and was used as a whitening cosmetic. However, the safety or efficacy of the above components in vivo is unclear, and thus their use is limited.

In particular, in recent years, not only functional aspects of cosmetics, but also user-friendly aspects such as irritation or toxicity have been emphasized as important. As an alternative to this, substances extracted from natural products are used in cosmetics as functional active ingredients, but in this case, functional effects may be reduced. Therefore, there is a need to develop a cosmetic composition that is highly functional and less irritating to human skin.

Korean Registered Patent Publication No. 10-1626437

Thus, the problem to be solved by the present invention is to provide a vegan cosmetic composition that does not irritate the skin while improving functionality such as moisturizing, whitening, and antioxidant, including only organic vegetable extracts as active ingredients.

Another problem to be solved by the present invention is to provide a method for producing the vegan cosmetic composition as described above.

The tasks of the present invention are not limited to the technical tasks mentioned above, and other technical tasks not mentioned will be clearly understood by those skilled in the art from the following description.

The cosmetic composition according to an embodiment of the present invention for solving the above problems includes at least one of Portulaca Oleracea extract, Lactobacillus, Shinyang cherry tree fruit extract, tart cherry extract, astaxanthin and hyaluronic acid. do.

The cosmetic composition of the present invention may be a composition for preparing non-irritating or hypoallergenic vegan cosmetics containing only organic botanical extracts as active ingredients.

The purslane extract, the cherry tree fruit extract, and the tart cherry extract may be extracted using known solvents and methods. Known solvents and methods may refer to methods such as extraction, distillation, and vacuum concentration using solvents such as water, alcohol, ketone, ether, and benzene, but are not limited thereto.

In one embodiment, the purslane extract, the cherry tree fruit extract, and the tart cherry extract may be extracted at a concentration of 30-50 wt%.

Lactobacillus may include at least one of Lactobacillus rhamnosus, Lactobacillus plantarum, L. acidophilus and L. casei. It may be, specifically, it may be a mixture containing the above four species. Such Lactobacillus can act as a biome technology in the cosmetic composition to increase safety and skin absorption.

In an exemplary embodiment, the purslane extract is a fermented extract and may be a mixture containing Lactobacillus together (Portulaca Oleracea Ferment Extract).

Hyaluronic acid is hyaluronic acid, hydrolyzed hyaluronic acid, sodium hyaluronate, sodium hyaluronate crosspolymer, hydroxypropyltrimonium hyaluronate ( hydroxypropyltrimonium hyaluronate), sodium acetylated hyaluronate, and hydrolyzed glycosaminoglycans. may be a mixture of

The composition may further contain additives such as humectants, preservatives/preservatives, emulsifiers, stabilizers, and the like. In a specific embodiment, the composition may further include at least one of caprylic/capric triglyceride, butylene glycol, 1,2-hexanediol, and glycerin. .

In some embodiments, the composition may further include one or more selected from the group consisting of wormwood, sambaekcho, pork potato, perilla leaf, morel cabbage, chives, and kujippong. In an exemplary embodiment, in the case of the cosmetic composition of the present invention further comprising wormwood, sambaekcho, pork potato, perilla leaf, morel cabbage, leek, and kkujippong, even though the content of all active ingredients is substantially the same, moisturizing ability and transdermal moisture Loss prevention capability can be further improved.

In one embodiment, the composition may further include an oleosome prepared or formed using lecithin. Oleosomes may be substances that easily absorb active ingredients into skin barriers or cells by forming particles in which phospholipids of lecithin enclose the outside of water-soluble or fat-soluble active ingredients in a single or double membrane. In some embodiments, the lecithin may be hydrogenated lecithin.

The composition comprising the oleosome particle(s) composed of the lecithin is at least one of polyglyceryl-10 oleate and polyglyceryl-3 methylglucose distearate. It may further include, and the substances penetrate through the phospholipid layer of the oleosome and are bound thereto to stabilize the structure of the oleosome and to make the delivery of active ingredients more effective.

In one embodiment, the composition including the oleosome particles may further include one or more novel peptides of SEQ ID NOs: 1 and 2. That is, the present inventors have found that the addition of a novel peptide having the amino acid sequence of SEQ ID NO: 1 and/or 2 can increase the effect of active ingredients to a level equal to or higher than that of the polyglyceryl-based substances.

Components included in the cosmetic composition according to the embodiments of the present invention as described above may have a composition shown in Table 1 below.

ingredient Content (wt%) purslane extract 5-30 Lactobacillus 1.0 x 10 ^4-7 (cells/50mL) Xinyang Cherry Tree Fruit Extract 1-10 tart cherry extract 0.1-5 astaxanthin 0.05-2 hyaluronic acid 0.1-5 Caprylic/Capric Triglyceride 0.1-5 butylene glycol 1-5 1,2-Hexenediol 1-10 glycerin 1-5 lecithin 1-10 Polyglyceryl-10 oleate 0.05 Polyglyceryl-3 Methylglucose Distearate 0.01 Novel Peptide 1 and/or 2 0.01 water balance

However, the above composition is not an essential composition, and even when some component(s) are omitted, it can be used as a cosmetic composition capable of solving the problems of the present invention.

Method for producing a cosmetic composition according to an embodiment of the present invention for solving the above other problems is purslane extract, lactobacillus, Shinyang cherry tree fruit extract, tart cherry extract, astaxanthin, hyaluronic acid and lecithin, polyglyceryl- 10 Mixing at least one of oleate, polyglyceryl-3 methylglucose distearate, the peptide of SEQ ID NO: 1 and the peptide of SEQ ID NO: 2 at 50-80 ° C. for 5-25 minutes (with a homogenizer) ; passing the mixture through a microfluidizer at high pressure (100-2,000 bar); cooling the passed mixture to 5-35 °C; and defoaming the cooled mixture.

In this way, a cosmetic composition comprising lecithin oleosome particles surrounding at least one active ingredient selected from purslane extract, lactobacillus, cherry blossom fruit extract, tart cherry extract, astaxanthin and hyaluronic acid can be obtained. At this time, the active ingredient may be contained in the inner hollow of the spherical oleosome particle.

Lecithin to form oleosomes is obtained by culturing soybean powder (10-30 h, 40-70 ° C, 50-200 rpm); filtration step; cell lysis step; and centrifugation (2,000-6,000 x g, 10-60 min, 1-10 °C).

Other embodiment specifics are included in the detailed description.

The cosmetic composition according to the embodiments of the present invention includes only organic vegetable extracts such as purslane extract as an active ingredient, improves functionalities such as moisturizing, whitening, and antioxidant, while not irritating the skin, and biome using lactobacillus. Safety and skin absorption can be improved by introducing technology.

Effects according to the embodiments of the present invention are not limited by the contents exemplified above, and more diverse effects are included in the present specification.

Advantages and features of the present invention, and how to achieve them, will become clear with reference to the detailed description of the following embodiments. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in a variety of different forms, and only the embodiments make the disclosure of the present invention complete, and those skilled in the art in the art to which the present invention belongs It is provided to fully inform the person of the scope of the invention, and the invention is only defined by the scope of the claims.

Terminology used herein is for describing the embodiments and is not intended to limit the present invention. In this specification, 'and/or' includes each and every combination of one or more of the recited items. In addition, singular forms also include plural forms unless otherwise specified in the text. The terms 'comprises' and/or 'comprising' used in the specification do not exclude the presence or addition of one or more other elements other than the mentioned elements. Numerical ranges expressed using '-' or 'to' represent numerical ranges including the values listed before and after them as lower and upper limits, respectively, unless otherwise specified. 'About' or 'approximately' means a value or range of values within 20% of the value or range of values set forth thereafter.

Also, terms such as first, second, A, B, (a), and (b) may be used to describe components of an embodiment of the present invention. These terms are only used to distinguish the component from other components, and the nature, order, or order of the corresponding component is not limited by the term.

Unless otherwise defined, all terms (including technical and scientific terms) used in this specification may be used in a meaning commonly understood by those of ordinary skill in the art to which the present invention belongs. In addition, terms defined in commonly used dictionaries are not interpreted ideally or excessively unless explicitly specifically defined.

And, in describing the embodiments of the present invention, if it is determined that a detailed description of a related known configuration or function hinders understanding of the embodiments of the present invention, the detailed description will be omitted.

As used herein, 'improvement' refers to any action that improves or beneficially changes a symptom in an individual, and includes, for example, (a) inhibition of the development (exacerbation) of a symptom or disease, (b) alleviation of a symptom or disease, or (c) means the elimination of a symptom or disease.

In the present specification, 'individual' or 'subject' means a cell, tissue, animal, or human to diagnose, prevent, or treat a specific disease or disorder using the present invention.

In the present specification, 'control group' refers to normal cells or patients that do not have or are not induced by a specific disease or disease.

In the present specification, 'level' may also mean a content, concentration, etc. that can be confirmed by measuring or analyzing the amount of a specific factor.

In this specification, the general rules for naming amino acid sequences may be based on either three-letter or one-letter amino acid codes unless there are specifically indicated exceptions. For example, the center of the amino acid structure is indicated by a three-letter code (eg, Ala, Lys), and the D-conformation (eg, D-Ala, D-Lys) is specified by writing "D-" in front of the three-letter code. If not indicated, L-conformation can be assumed. Amino acid residues constituting a protein or peptide may be natural or non-natural amino acid residues.

Hereinafter, embodiments of the present invention will be described in detail through preparation examples and experimental examples, but it is obvious that the effects of the present invention are not limited by the following experimental examples.

Preparation Example 1: Preparation of the cosmetic composition of the present invention (1)

Purslane extract, lactobacillus, cherry tree fruit extract, tart cherry extract, hyaluronic acid, astaxanthin, caprylic/capric triglyceride, butylene glycol, 1,2-hexenediol and water were prepared in Table 2 below. 50 mL of the cosmetic composition of Examples 1 to 4 and Comparative Example was prepared by mixing with the same composition.

Purslane extract, Shinyang cherry tree fruit extract, and tart cherry extract were extracted at concentrations of 41, 43, and 38 wt%, respectively, using known solvents and methods.

As for the lactobacillus, a mixture of Lactobacillus rhamnosus, Lactobacillus plantarum, L. acidophilus and L. casei strains was used. .

(wt%) Example 1 Example 2 Example 3 Example 4 comparative example purslane extract 10 - 10 20 - Lactobacillus (cells) 1.0 x 10 ⁵ - - 1.0 x 10 ⁵ - Xinyang Cherry Tree Fruit Extract 5 10 5 - - tart cherry extract One 10 One - - astaxanthin 0.1 0.1 0.1 0.1 - hyaluronic acid One One One One One Caprylic/Capric Triglyceride 0.5 0.5 0.5 0.5 0.5 butylene glycol 2 2 2 2 2 1,2-Hexenediol 3 3 3 3 3 water balance

Preparation Example 2: Preparation of the cosmetic composition of the present invention (2)

A novel peptide was synthesized through a known Fmoc solid phase peptide synthesis (SPPS) method (Wang C. Chan, Perter D. White, 'Fmoc-solid phase peptide synthesis', Oxford). As a coupling reagent, HBTU [2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetamethylaminium hexafluorophosphate] / HOBt (N-Hydroxxybenzotriazole) / NMM (4-Methylmorpholine) are used did For Fmoc removal, piperidine in DMF was used in 20% DMF. Cleavage Cocktail [TFA (trifluoroacetic acid) / TIS (triisopropylsilane) / EDT (ethanedithiol) / H O = 92.5 / 2.5 / 2.5 / 2.5] was used to separate the synthesized peptide from the amino acid resin and remove the protecting group from the residue. . Each peptide was synthesized by sequentially reacting the corresponding amino acids according to the sequence of each peptide in a state where the starting amino acid to which the amino acid protecting group was bound was bound to the solid phase support, washing with a solvent, and repeating the deprotection process. After cutting off the synthesized peptide from the resin, it was purified by C18 reverse-phase high-performance liquid chromatography (HPLC, Waters Associates, USA), and the success of the synthesis was confirmed by LC/MS, followed by freeze-drying. The purity of the synthesized peptide was 95% or higher as a result of high-performance liquid chromatography.

New peptides synthesized according to the above method are shown in Table 3 below.

sequence number amino acid sequence One RKKRRQRKTHG 2 RKKRRQTSVG

Hydrogenated lecithin, polyglyceryl-10 oleate, polyglyceryl-3 methylglucose distearate, and novel peptides in the cosmetic composition of Example 1 1 and 2 were mixed with a homogenizer at 60 ° C. for 15 minutes with the composition shown in Table 4, and then continuously passed through a microfluidizer at high pressure, followed by cooling and defoaming to obtain a cosmetic composition containing lecithin oleosomes. manufactured. Hydrogenated lecithin to form oleosomes was obtained by incubation of soybean powder (20 h, 57 °C, 150 rpm), filtration, cell lysis, and centrifugation (4,000 x g, 30 min, 4 °C) sequentially. It was prepared through a method including

(wt%) Example 5 Example 6 lecithin 5 5 glycerin 3 3 Polyglyceryl-10 oleate 0.05 - Polyglyceryl-3 Methylglucose Distearate 0.01 - Novel peptide 1, 2 - 0.01

Experimental Example 1: Evaluation of cytotoxicity

In order to evaluate the cytotoxicity of the cosmetic composition of the present invention, it was tested as follows.

Using a 96-well plate, human keratinocyte cell line (HaCaT) was inoculated and cultured in DMEM (Dulbecco's modified Eagle's medium) medium containing 1% FBS (Fetal bovine serum). When the cells were filled to about 60% in the culture dish, the culture medium was changed to medium without serum and cultured for 16-24 hours, and then the medium was replaced with medium containing the above composition. After 48 hours, the medium was replaced with a medium containing 10% MTT (Tetrazolium-based colorimetric) solution. After culturing for 2-4 hours, the medium was removed and the precipitate remaining at the bottom of the culture dish was dissolved with DMSO (Dimethyl sulfoxide). The absorbance at 570 nm was measured with an ELISA reader and the average value was calculated, and the results are shown in Table 5 below.

comparative example Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Relative cell viability (%) 100 103 98 102 103 104 99

As shown in Table 5, it can be seen that the cosmetic composition of the present invention is suitable for use as a non-irritating cosmetic because it does not exhibit toxicity to keratinocytes.

Experimental Example 2: Evaluation of moisturizing ability and transepidermal water loss

The compositions were formulated and applied to the skin of the experimental group, and after 12 hours, the moisturizing power was measured using CORNEOMETER CM 850 (Courage Khazaka electronic GmbH), and the transepidermal water loss (TEWL, Trans Epidermal Water Loss) was measured using a TEWL meter. , The results were shown in Table 6 below.

comparative example Example 1 Example 2 Example 3 Example 4 moisturizing degree 70 85 75 81 83 TEWL 12 8 10 9 7

As shown in Table 6, it can be seen that the cosmetic composition of the present invention has excellent moisturizing power and water holding power. In addition, since the moisturizing degree and TEWL of Example 1 are superior to Examples 2 to 4, synergistic effect (synergistic effect) can be seen. In particular, the difference between Examples 1 and 3 may be due to the difference in absorption capacity of the cosmetic composition through the introduction of biome technology using Lactobacillus.

Experimental Example 3: Evaluation of antioxidant activity

In order to evaluate the antioxidant activity of the cosmetic composition of the present invention, the nitric oxide (NO) scavenging ability was tested as follows.

Raw 264.7 macrophages were inoculated into a 96-well plate at a concentration of 1 x 10 ⁶ cell/mL in DMEM medium supplemented with 10% FBS and 100 units/ml of penicillin and streptomycin, and incubated at 37 °C and 5% CO ₂ . Incubated overnight. The next day, after removing the medium and replacing it with a fresh medium, 50 μl of the above composition was applied to the cells. After culturing in a CO ₂ incubator for 30 minutes, 50 μl (1 μg/ml) of LPS (lipopolysaccharide)-containing medium was treated and cultured in an incubator (5% CO ₂ , 37 °C) for 24 hours. Phosphate buffered saline (PBS) was used as a control. Griess reagent I (NED solution) and Griess reagent II (Sulfaniliamide solution) were mixed in equal amounts, reacted for 10 minutes, and then optical density at 540 nm was measured using a microplate reader. The concentration of nitric oxide (NO) was calculated using a standard curve (0-100 μM) of sodium nitrite. The results were shown in Table 7 below.

division NO production (%) control group 100 comparative example 86 Example 1 73 Example 2 79 Example 3 77 Example 4 80 Example 5 71 Example 6 69

As shown in Table 7, it can be seen that the cosmetic composition of the present invention has excellent antioxidant and anti-inflammatory activity because it reduces the amount of NO produced by LPS by 20-30%. In addition, since the amount of NO produced in Example 1 was lower than that of Examples 2 to 4, it was found that a synergistic effect was exhibited when all of the purslane extract, lactobacillus, cherry tree fruit extract, and tart cherry extract were used when there was no difference in total content. And since the amount of NO produced in Examples 5 and 6 is lower than that in Example 1, it can be seen that the components included in the composition can act more effectively on cells when delivered through oleosomes. In particular, the difference between Examples 1 and 3 may be due to the difference in absorption capacity of the cosmetic composition through the introduction of biome technology using Lactobacillus.

Experimental Example 4: Evaluation of melanin production

In order to evaluate the whitening activity of the cosmetic composition of the present invention, the ability to inhibit melanin formation was evaluated by analyzing the amount of melanin produced.

Melanoma cell line (B16F10) cells were inoculated in a 6-well plate and cultured for 24 hours. α-MSH (Sigma-Aldrich, USA) hormone that promotes melanin formation and the above compositions were injected into the medium. After 48 hours, the cells were harvested, washed once with PBS, 120 μL of 1 N NaOH lysis buffer was added, and the cells were lysed by heating at 95 ° C. for 10 minutes. Absorbance was measured at a wavelength of 450 nm using a microplate reader, and arbutin at a concentration of 100 mg/L was used as a positive control. The melanin production inhibition rate was calculated according to Equation 1 below, and the evaluation results were shown in Table 8 below.

[Equation 1]

Melanin production inhibition rate (%) = 100 x [1 - (absorbance of each sample / absorbance of negative control group)]

division Melanin production inhibition rate (%) positive control 25 comparative example 13 Example 1 28 Example 2 26 Example 3 27 Example 4 23 Example 5 30 Example 6 31

As shown in Table 8, it can be seen that the cosmetic composition of the present invention has excellent whitening activity because the melanin production inhibition rate is equal to or greater than that of the positive control group. In addition, since the melanin production inhibition rate of Example 1 was higher than that of Examples 2 to 4, it was confirmed that there was a synergistic effect when all of the purslane extract, lactobacillus, cherry tree fruit extract, and tart cherry extract were used when there was no difference in total content. As can be seen, since the melanin production inhibition rate of Examples 5 and 6 is higher than that of Example 1, it can be seen that the components included in the composition can act more effectively on cells when delivered through oleosomes.

Although the above has been described with reference to the embodiments of the present invention, this is only an example and does not limit the present invention, and those skilled in the art to which the present invention belongs will be able to It will be appreciated that various modifications and applications not exemplified above are possible. For example, each component specifically shown in the embodiment of the present invention can be modified and implemented. And differences related to these modifications and applications should be construed as being included in the scope of the present invention as defined in the appended claims.

Claims (4)

purslane extract;
Lactobacillus;
Xinyang cherry tree fruit extract;
tart cherry extract;
astaxanthin; and
containing hyaluronic acid
cosmetic composition. The method of claim 1,
oleosome particles comprising lecithin; and
Further comprising at least one of polyglyceryl-10 oleate and polyglyceryl-3 methylglucose distearate
cosmetic composition. The method of claim 2,
Further comprising at least one of the peptide of SEQ ID NO: 1 and the peptide of SEQ ID NO: 2
cosmetic composition. Purslane extract, Lactobacillus, Shinyang cherry tree fruit extract, Tart cherry extract, astaxanthin, hyaluronic acid and lecithin, polyglyceryl-10 oleate, polyglyceryl-3 methylglucose distearate, the peptide of SEQ ID NO: 1 and mixing at least one of the peptides of SEQ ID NO: 2 at 50-80 ° C for 5-25 minutes;
passing the mixture through a microfluidizer under high pressure;
cooling the passed mixture to 5-35 °C; and
Comprising the step of defoaming the cooled mixture
A method for producing a cosmetic composition for moisturizing, whitening and antioxidation.