KR102311325B1 - Cosmetic Composition for Moisturizing or Improving Elasticity of Skin Comprising Low Molecular and Polar Keratin Peptides as Active Ingredients - Google Patents

Abstract

The present application relates to a cosmetic composition for moisturizing or enhancing skin elasticity comprising a polar low molecular weight keratin peptide as an active ingredient. The cosmetic composition for skin moisturizing or elasticity enhancement comprising a polar low molecular weight keratin peptide as an active ingredient according to an aspect of the present invention promotes elastase inhibitory ability and procollagen type-1 C peptide (PIP) synthesis for fibroblasts and α-elastin synthesis accelerating ability, and has excellent effects of improving skin elasticity, skin torsion elasticity, skin moisture content and skin color when applied to human skin, so it can be usefully used as a cosmetic for moisturizing or enhancing skin elasticity.

Description

Cosmetic Composition for Moisturizing or Improving Elasticity of Skin Comprising Low Molecular and Polar Keratin Peptides as Active Ingredients

The present invention relates to a cosmetic composition for moisturizing or enhancing skin elasticity comprising a polar low molecular weight keratin peptide as an active ingredient.

The skin protects the human body from physical and chemical stimuli from the external environment, and is largely divided into three layers: the epidermis, dermis, and subcutaneous fat tissue. In particular, the skin contains about 65 to 70% of the moisture of the human body, which transports various physiologically active substances necessary for the human body and helps the skin to maintain a moist state. In addition, the skin plays an important role in controlling the evaporation of water out of the body.

Among them, the epidermis is divided into a stratum corneum, a granular layer, a stratum corneum, and a basal layer. The stratum corneum of the epidermis contains about 10 to 20% of water and is present on the outermost part of the human body to form a natural protective film made of sebum from the sebaceous glands and sweat from the sweat glands to prevent moisture evaporation from the body and penetration of external substances. to block A high concentration of natural moisturizing factor (NMF), a water-soluble component, exists in the cells constituting the stratum corneum, helping the skin to show flexibility and maintaining proper moisture. However, due to various causes, such as changes in the environment or lifestyle, artificial temperature control of cooling/heating, skin stress due to various stresses and environmental pollution, frequent washing according to makeup habits, and natural skin aging due to increasing age. As a result, the moisture of the stratum corneum decreases, the skin becomes dry and the surface becomes rough, and the skin loses its luster and looks dull.

Keratin is a protein that forms the basis of epithelial structures such as hair, nails, and skin, and forms the epidermal layer of the skin. However, when keratin is degenerated such as carbonylation, it lowers the division of keratinocytes, which slows the formation of new cells, weakening the skin barrier function, increasing water loss through the skin, and cause a decrease in elasticity.

Therefore, although research on functional cosmetics for protecting the skin barrier and enhancing skin elasticity has been made in various fields, there is an urgent need to develop a cosmetic composition that has the effect of moisturizing or enhancing skin elasticity and has no side effects to the human body.

Recently, as a polymer of amino acids, it is structurally simpler than proteins, but because the interaction with proteins in the body is very large, various peptides that have an anti-aging effect by enhancing or inhibiting the activity of enzymes, that is, vegetable peptides, synthetic peptides , microbial-derived peptides, etc., are being developed for cosmetic materials. Korean Patent Registration No. 10-0862979 discloses a collagen peptide composition comprising collagen peptides extracted from vegetable or marine raw materials individually or in a uniform ratio, which is used for moisturizing or wrinkle improvement. A collagen peptide composition and a method for preparing the same are described. However, in the case of plant peptides, the process of separating and purifying peptides from plants is complicated, and it is difficult to obtain materials in large quantities. On the other hand, in the case of synthetic peptides, for industrialization, they are inevitably behind in price competitiveness due to the cost of synthesis, and there is a problem of cost burden when used as a raw material for functional cosmetics.

Accordingly, the present inventors prepared a natural keratin peptide through a strain producing a keratin peptide degrading enzyme while researching a cosmetic composition for moisturizing or enhancing skin elasticity. In particular, when a polar low molecular weight keratin peptide is used as a cosmetic composition, it has been found that the skin moisturizing or elasticity enhancing effect is excellent, and the present invention has been completed.

One technical problem to be achieved by the present invention is to provide a cosmetic composition for moisturizing or enhancing skin elasticity using a polar low molecular weight keratin peptide.

Another technical problem to be achieved by the present invention is to provide a method for producing a polar low molecular weight keratin peptide for skin moisturizing or elasticity enhancement.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. There will be.

In order to achieve the above technical problem, an embodiment of the present invention,

Provided is a cosmetic composition for moisturizing or enhancing skin elasticity comprising a polar low molecular weight keratin peptide as an active ingredient.

In an embodiment of the present invention, the polar low molecular weight keratin peptide may be one obtained by culturing Fervidobacterium islandicum AW-1.

In an embodiment of the present invention, the Pervidobacterium islandicum AW-1 may be characterized in that it is cultured in a medium containing chicken hair.

In an embodiment of the present invention, the polar low molecular weight keratin peptide may have a molecular weight of 10 kDa or less.

In an embodiment of the present invention, the polar low molecular weight keratin peptide may be obtained by fractionating a keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent.

In an embodiment of the present invention, the polar low molecular weight keratin peptide may be included in an amount of 0.0001 to 100 wt% based on the total weight of the cosmetic composition.

In an embodiment of the present invention, the keratin peptide exhibits any one activity selected from elastase inhibitory ability, procollagen type-1 C peptide (PIP) synthesis promoting ability, and α-elastin synthesis promoting ability. It may be characterized by

In an embodiment of the present invention, the cosmetic composition has a formulation selected from the group consisting of lotion, lotion, cream, essence, pack, emulsion, oil gel, serum, cleansing foam, makeup base and foundation. can

In order to achieve the above technical problem, another embodiment of the present invention is

Fervidobacterium islandicum ( Fervidobacterium islandicum ) A first step of culturing AW-1 in a medium containing chicken hair to obtain a keratin peptide stock solution;

a second step of separating the polar keratin peptide mother liquid from the keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent;

A third step of separating the polar low molecular weight keratin peptide mother liquid having a molecular weight of 10 kDa or less by passing the polar keratin peptide mother liquid through a membrane having a pore size of 10 kDa,

Provided is a method for producing a polar low-molecular-weight keratin peptide for skin moisturizing or elasticity enhancement.

According to an embodiment of the present invention, the cosmetic composition comprising the polar low molecular weight keratin peptide of the present invention as an active ingredient has an excellent effect in enhancing skin elasticity as well as moisturizing the skin.

The cosmetic composition according to the present invention exhibits an ability to inhibit elastase, an ability to promote procollagen type-1 C peptide (PIP) synthesis, and an ability to promote synthesis of α-elastin with respect to fibroblasts.

In addition, according to an embodiment of the present invention, the present invention has the effect of improving skin elasticity, skin torsion elasticity, skin moisture content and skin color, and thus overall satisfaction is increased, so that it can be usefully used as a functional skin cosmetic.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a schematic diagram illustrating a process for isolating a keratin peptide using Fervidobacterium islandicum AW-1 according to an embodiment of the present invention.
Figure 2 is a diagram showing the elastase (Elastase) inhibitory activity rate of the keratin peptide prepared according to an embodiment of the present invention.
3 is a diagram showing the procollagen type IC peptide synthesis ability of keratin peptides prepared according to an embodiment of the present invention.
4 is a diagram showing the α-elastin producing ability of the keratin peptide prepared according to an embodiment of the present invention.
5 is a diagram showing the skin elasticity improvement rate of the keratin peptide prepared according to an embodiment of the present invention.
6 is a diagram showing the improvement rate of skin torsion elasticity of the keratin peptide prepared according to an embodiment of the present invention.
7 is a diagram showing the improvement rate of skin moisture content of the keratin peptide prepared according to an embodiment of the present invention.
8 is a diagram showing the skin color improvement rate of the keratin peptide prepared according to an embodiment of the present invention.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in several different forms, and thus is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a part is said to be “connected (connected, contacted, coupled)” with another part, it is not only “directly connected” but also “indirectly connected” with another member interposed therebetween. "Including cases where In addition, when a part "includes" a certain component, this means that other components may be further provided without excluding other components unless otherwise stated.

The terms used herein are used only to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

The first aspect of the present application is

Provided is a cosmetic composition for moisturizing or enhancing skin elasticity comprising a polar low molecular weight keratin peptide as an active ingredient.

Hereinafter, a cosmetic composition for moisturizing or enhancing skin elasticity comprising the polar low molecular weight keratin peptide as an active ingredient according to the first aspect of the present application will be described in detail.

Keratin peptide stock solution

The keratin peptide may be a raw material containing a keratin protein separated from a keratin peptide mother liquid obtained by culturing the Pervidobacterium islandicum AW-1.

The Fervidobacterium islandicum (Fervidobacterium islandicum) AW-1 (KFCC 11115) is an extremophilic microorganism, a highly thermophilic bacteria grown at a high temperature of 40 to 80 ° C., and an enzyme that decomposes feathers, hair, etc. of chickens. can produce

The raw material containing the keratin protein may be chicken feathers (chicken feathers). The chicken hair is an insulator accounting for 5 to 7% of the total weight of the chicken, and more than 90% is characterized in that it is composed of keratin, the most abundant insoluble protein in nature. Keratin is divided into soft keratin and hard keratin according to the cysteine content. Soft keratin has about 10 to 14% cysteine and is contained in chicken hair with many disulfide bonds. The keratin contained in chicken hair has a structure of α-helix (α-keratin) or β-sheet (β-keratin) that is entangled with each other, so protein chains are stacked very tightly, and numerous disulfide bonds and hydrogen bonds and It has a complex structure in which hydrophobic interactions exist, and numerous types of keratin peptides can be formed according to the treatment with degrading enzymes. Disclosed herein is a keratin peptide having a new structure and composition obtained by adding and culturing a specific strain to a medium containing chicken hair as a nitrogen source. Among them, the polar low molecular weight keratin peptide shows a desired skin moisturizing or elasticity enhancement effect.

The nitrogen source, chicken hair, is preferably contained in the medium at 5 to 15 g/L, more preferably at 8 g/L. When the content is included within the above range, keratin peptide production through anaerobic culture is possible within 1 to 2 days, and it is more preferable to obtain functional keratin peptide of an appropriate size.

As an example of a method for obtaining the keratin peptide mother liquor, 5 to 15 g/L, preferably 8 g/L, of chicken hair is added as a nitrogen source to a growth medium having the composition shown in Table 1 below, and at 72° C., Pervidobacterium islandicum After filtration of the culture supernatant obtained by anaerobic fermentation of AW-1, centrifugation of the filtered culture supernatant at 10,000 × g to precipitate and recover the cells, filtration and concentration to prepare keratin peptides. can In addition, if necessary, precipitation or filtration may be repeated. Table 1 is exemplary, and the same effect may be exhibited even if the addition amount of each component is adjusted in the range of ±10%, and only a part of the medium component may be included.

medium ingredients Addition (g/L) NaCl 2.0 CaCl ₂ 2H ₂ O 0.5 MgSO ₄ 7H ₂ O 0.5 MgCl ₂ 6H ₂ O 0.5 KH ₂ PO ₄ 0.5 K ₂ HPO ₄ 0.1 (NH ₄ ) ₂ SO ₄ 0.5 KCl 0.1 KBr 0.1 H ₃ BO ₃ 0.03 SrCl ₂ 6H ₂ O 0.03 Na ₂ WO ₄ 0.033mg Na ₂ SeO ₃ 0.026mg yeast extract 1.0 chicken hair 8.0 Na ₂ S (25%, w/v) 3ml/L

Isolation of Polar Keratin Peptides

The polar keratin peptide of the present invention may be one obtained by separating the polar keratin peptide from the mother liquor. Specifically, the polar keratin peptide of the present invention may be obtained by separating the polar keratin peptide mother liquid from the keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent.

Representative non-polar solvents for use in the present invention can be any non-polar solvent known in the art. For example, it may be at least one selected from the group consisting of alkane, cycloalkane, alkene, aldehyde, ketone, ether, ester, amine, aromatic, silane, silicone, and carbon dioxide. Specifically, propane, 2,2-dimethylpropane, butane, 2,2-dimethylbutane, pentane, isopropyl ether, hexane, triethylamine, heptane, octane, nonane, decane, isobutyl isobutyrate, tributylamine, Undecane, 2,2,4-trimethylpentyl acetate, isobutyl heptyl ketone, diisobutyl ketone, cyclopentane, cyclohexane, isobutylbenzene, n-nonylbenzene, n-octylbenzene, n-butylbenzene, p- the group consisting of xylene, ethylbenzene, 1,3,5-trimethylbenzene, m-xylene, toluene, o-xylene, decene, docenene, tetradecene and heptadecanal It may be one or more selected from. More specifically, it may be at least one selected from pentane, hexane, and heptane.

A representative polar solvent used in the present invention may be, for example, any one or more selected from water, C1-6 alcohol, C1-6 aldehyde, and C1-6 ketone. Specifically, it may be at least one solvent selected from methanol, ethanol, propanol, butanol, isopropanol, ethyl acetate, acetone, and chloroform.

According to one embodiment not limited herein, the polar solvent may be distilled water and the non-polar solvent may be hexane. Specifically, the polar keratin peptide of the present invention may be fractionated into distilled water when the keratin peptide mother liquid is added to a mixed solution of distilled water and hexane in a volume ratio of 1.0: 0.5 to 2.0.

The polar keratin peptide may have excellent ability to inhibit elastase, procollagen type-1 C peptide (PIP) synthesis, and α-elastin synthesis, unlike the non-polar to low polar keratin peptide. .

Isolation of low molecular weight keratin peptides

Herein, the low molecular weight keratin peptide may be one obtained by separating the low molecular weight keratin peptide from the mother liquor. Specifically, the low molecular weight keratin peptide may have a molecular weight of 10 kDa or less.

Specifically, the term "low molecular weight keratin peptide" may refer to purifying and using only low molecular weight peptides having a molecular weight of 10 kDa or less obtained through ultrafiltration of keratin peptides. On the other hand, "polymer keratin peptide" may be a material obtained from the supernatant remaining after ultrafiltration of the keratin peptide, and may have a molecular weight of more than 10 kDa.

Unlike keratin peptides with a molecular weight of 10 kDa or less, keratin peptides with a molecular weight of 10 kDa or less can have excellent ability to inhibit elastase, procollagen type-1 C peptide (PIP) synthesis, and α-elastin synthesis. have.

cosmetic composition

The polar low molecular weight keratin peptide may be included in an amount of 0.0001 to 100 wt%, specifically 1 to 10 wt%, more specifically 3 to 5 wt%, based on the total weight of the cosmetic composition. When the content of the keratin peptide is lower than the above range, the content of the polar low molecular weight keratin peptide as an active ingredient is too low, so the skin moisturizing or elasticity enhancing effect may be insufficient. can

In addition, the polar low molecular weight keratin peptide exhibits an ability to inhibit elastase, an ability to promote procollagen type-1 C peptide (PIP) synthesis, and an ability to promote α-elastin synthesis. It is effective in improving elasticity, skin moisture content and skin color.

Furthermore, the cosmetic composition for skin moisturizing or elasticity enhancement may further include conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc. commonly used in cosmetic compositions in addition to the polar low molecular weight keratin peptide. have. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, and allantoin. .

Since the cosmetic composition of the present invention is basically applied to the skin, it may be prepared in any formulation conventionally prepared with reference to a cosmetic composition in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. The present invention is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray, or a powder.

When the formulation of the cosmetic composition for skin moisturizing or elasticity enhancement is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc as a carrier component , zinc oxide and the like may be included.

When the formulation of the cosmetic composition for skin moisturizing or elasticity enhancement is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included as carrier components, and in particular, in the case of a spray, additionally propellants such as chlorofluorohydrocarbons, propane/butane, dimethyl ether, and the like.

When the formulation of the cosmetic composition for skin moisturizing or elasticity enhancement is a solution or emulsion, a solvent, solubilizer, emulsifier, etc. may be included as carrier components, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl Alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, fatty acid esters of sorbitan, and the like may be included.

When the formulation of the cosmetic composition for moisturizing or enhancing skin elasticity is a suspension, a liquid diluent such as water, ethanol, or propylene glycol as a carrier component; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tracanth, and the like may be included.

When the formulation of the cosmetic composition for skin moisturizing or elasticity enhancement is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate as carrier components , sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, and the like.

The second aspect of the present application is

Fervidobacterium islandicum ( Fervidobacterium islandicum ) A first step of culturing AW-1 in a medium containing chicken hair to obtain a keratin peptide stock solution;

a second step of separating the polar keratin peptide mother liquid from the keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent;

A third step of separating the polar low molecular weight keratin peptide mother liquid having a molecular weight of 10 kDa or less by passing the polar keratin peptide mother liquid through a membrane having a pore size of 10 kDa,

Provided is a method for producing a polar low-molecular-weight keratin peptide for skin moisturizing or elasticity enhancement.

The first step is to obtain a keratin peptide stock solution, the second step is to isolate the polar keratin peptide, and the third step is to isolate the low molecular weight keratin peptide, as described above.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention.

Example 1. Preparation of Keratin Peptides

As shown in FIG. 1, for the preparation of a keratin peptide mixture, a chicken hair decomposition strain, Fervidobacterium islandicum AW-1, as a nitrogen source, chicken hair (8 g / L) was added to a growth medium at 72 ° C. The culture supernatant (0.8%) was obtained by anaerobic culture in The culture supernatant was first filtered out of decomposed chicken feathers using filter paper (5㎛, No.20, Hyundai Micro, Korea), and then centrifuged at 10,000×g at 4°C for 20 minutes to recover the supernatant. The recovered supernatant (keratin peptide stock solution) was dried at 72° C. for 48 hours.

Sample 1. Keratin Peptide (AWB1)

The dried keratin peptide was stored at -80° C. and lyophilized for 96 hours to prepare sample 1 (keratin peptide, AWB1).

Sample 2. Polar Keratin Peptide (AWB2)

The dried keratin peptide was stored at -80°C and lyophilized for 96 hours. After freeze-drying, distilled water and hexane are added to a mixture of 1:1 volume ratio, shaken for 15 minutes or more, stopped in a separatory funnel, distilled water fractions are obtained, and freeze-drying By repeating, sample 2 (polar keratin peptide, AWB2) was prepared.

Sample 3. Low Molecular Keratin Peptide (AWB3) and Sample 6. Low Molecular Keratin Peptide (AWB6)

The dried keratin peptide was stored at -80°C and lyophilized for 96 hours. Ultrafiltration was used to conveniently fractionate ultra-low molecular weight keratin peptides of 1 kDa or less from the freeze-dried sample. For ultrafiltration, a membrane having a pore size of 10 kDa was used as the filtration membrane. Specifically, the sample was introduced into a filtration module equipped with a filtration membrane, and a pressure of about 10 to 30 psi was applied so that the sample was filtered while passing through the filtration membrane. Accordingly, sample 3 (low molecular weight keratin peptide, AWB3) having a molecular weight of 10 kDa or less was obtained, and sample 6 (high molecular weight keratin peptide, AW6) having a molecular weight of 10 kDa or more was obtained.

Sample 4. Polar low molecular weight keratin peptide (AWB4) and sample 5. Polar Polymer Keratin Peptide (AWB5)

The dried keratin peptide was stored at -80°C and lyophilized for 96 hours. After freeze-drying, polar keratin peptides were obtained using distilled water and hexane in the same manner as in Sample 2. The polar keratin peptide was subjected to ultrafiltration in the same manner as in Sample 3. Accordingly, sample 4 (polar low molecular weight keratin peptide, AWB4) having a molecular weight of 10 kDa or less was obtained, and sample 5 (high molecular weight keratin peptide, AW5) having a molecular weight of 10 kDa or more was obtained.

formulation example. Manufacture of mask pack containing polar polymer keratin peptide as an active ingredient

A mask pack was prepared using Sample 4 of Example 1 (polar low molecular weight keratin peptide, AWB4). Specifically, a mask pack filling emulsion was prepared according to the composition of Table 2 below.

Raw material product name INCI NAME %(W/W) function One Arlacel 165 Glyceryl Stearate/PEG-100 Stearate 1.00 emulsifier 2 HCO-40 PEG-40 Hydrogenated Costor Oil 0.50 emulsifier 3 Hicos CEH Cetyl Ethylhexanoate 1.00 emollient 4 Hicos GTC Caprylic/Capric Triglycerides 1.00 emollient 5 DC 200f/6CS Dimethicone 0.30 emollient 6 1,3 BG Butylene Glycol 8.00 moisturizer 7 D.I water I Water 77.16 8 EDTA-2Na Disodium EDTA 0.02 chelating agent 9 D-Panthenol Panthenol 0.20 moisturizer 10 KMO-6 1,2 Hexandiol 2.50 moisturizer 11 Glycerine Glycerin 6.00 moisturizer 12 Keltrol F Xanthan Gum 0.10 thickener 13 Aristoflex AVC Ammonium Acryloyldimethyltaurate/VP Copolymer 0.20 thickener 14 Hyaluronic Acid 1% solution Sodium Hyaluronate 1.00 moisturizer 15 Keratin Peptide 1% solution Keratin Peptide (Sample 4) 1.00 additive 16 Lavender Oil Lavandula Angustifolia (Lavender) Oil 0.02 Spices Total 100.00

First, the raw materials 1 to 6 were weighed, put into a 2L beaker, and dissolved by heating to 70° C. to prepare an oil phase. Then, the raw materials 7 to 11 were weighed, put into a 20L plastic container, and the raw materials 12-13 were dispersed using a Disper Mixer to prepare a water phase. The oil phase was added to the aqueous phase and emulsified for 10 minutes at about 1,500 rpm using a Disper Mixer. Here, the raw materials 14 to 16 were added and emulsified at about 1,500 rpm for 5 minutes using a Disper Mixer to prepare a mask pack filling emulsion.

A mask pack was prepared by putting 35 ml of the prepared emulsion into an airtight packaging container containing one mask pack sheet.

Comparative preparation example. Manufacture of mask pack that does not contain polar polymer keratin peptide

In Comparative Preparation Example, a mask pack was prepared in the same manner as in Preparation Example except that Sample 4 (polar low molecular weight keratin peptide, AWB4) was not included.

Experimental Example 1. Wrinkle improvement effect measurement experiment

Elastase assay was performed on Samples 1 to 6 prepared in Example 1 to compare elastase inhibitory activity rates. In order to measure the elastase inhibitory activity rate, 50 μl of each concentration sample and control were put into the well of a 96-well plate, and 190 μl of 1 mM N-succinyl-(L-Ala)3-p-nitroanilide was put into the well of the 96-well plate. 10 μl/well of porcine pancreas elastase was added and incubated at 25° C. for 20 minutes. Thereafter, the absorbance was measured at 405 nm to calculate the inhibitory activity rate. Elastase (Elastase) inhibitory activity rate results are shown in Table 3 and Figure 2 below.

content
sample 0.25% 0.5% One% sample 1 5% 10% 20% sample 2 6% 12% 24% sample 3 10% 20% 30% sample 4 20% 40% 75% sample 5 11% 22% 35% sample 6 7% 14% 30%

As shown in Table 3 and FIG. 2, the elastase inhibitory activity of sample 4 (polar low molecular weight keratin peptide) was the greatest, followed by polar high molecular weight keratin peptide, low molecular weight keratin peptide, high molecular weight keratin peptide, polar keratin peptide, and keratin peptide. Elastase inhibitory effect was observed. Through this, it can be confirmed that the polar low molecular weight keratin peptide has an elastase inhibitory activity in vitro.

Experimental Example 2. Collagen production effect measurement experiment

Samples 1 to 6 prepared in Example 1 were applied to human fibroblasts by concentration, and procollagen type I C peptide assay was performed to compare the procollagen type I C peptide synthesis ability. The results of procollagen type I C peptide synthesis are shown in Table 4 and FIG. 3 below.

content
sample 0.01% 0.02% 0.04% sample 1 2.1% 5% 10% sample 2 3% 6% 15% sample 3 7% 15% 30% sample 4 10% 20% 40% sample 5 6% 14% 28% sample 6 4% 8% 16%

As shown in Table 4 and FIG. 3, Sample 4 (polar low molecular weight keratin peptide) had the highest procollagen type IC peptide synthesis ability, followed by low molecular weight keratin peptide, polar high molecular keratin peptide, high molecular weight keratin peptide, polar keratin peptide, and keratin peptide. The synthesis ability of PIP Procollagen type IC peptide was high. Through this, it can be confirmed that the polar low molecular weight keratin peptide has the effect of increasing the synthesis of Procollagen type I C peptide in human fibroblasts.

Experimental Example 3. α-elastin synthesis increase effect measurement experiment

Samples 1 to 6 prepared in Example 1 were applied to human fibroblasts for each concentration, and elastin assay was performed to compare α-elastin producing ability. The results of α-elastin producing ability are shown in Table 5 and FIG. 4 below.

content
sample 0.01% 0.02% 0.04% sample 1 0.01% 0.04% 0.1% sample 2 0.2% 0.4% 0.8% sample 3 0.5% One% 2% sample 4 One% 2% 5% sample 5 0.02% 0.05% One% sample 6 0.015% 0.025% 0.5%

As shown in Table 5 and FIG. 4, Sample 4 (polar low molecular weight keratin) had the highest α-elastin synthesis ability, followed by low molecular weight keratin peptide, polar high molecular keratin peptide, high molecular weight keratin peptide, polar keratin peptide, high molecular weight keratin peptide, and keratin peptide. In that order, α-elastin synthesizing ability was found to be high. Through this, it can be confirmed that the polar low molecular weight keratin peptide has the effect of increasing α-elastin synthesis in human fibroblasts.

Experimental Example 4. Skin elasticity improvement effect human application test

After 44 adults were allowed to use Formulation Example and Comparative Formulation Example, skin elasticity of the cheek area was measured before use, 2 weeks after use, and 4 weeks after use. The measured skin elasticity improvement rate result values are summarized in Table 6 and FIG. 5 below.

Kinds 2 weeks after use 4 weeks after use Formulation example 11.4% 25.4% Comparative preparation example 8.4% 15.2%

As shown in the results of Table 6 and FIG. 5, the formulation example containing the polar low molecular weight keratin peptide showed a higher skin elasticity improvement rate 2 weeks after use and 4 weeks after use than the comparative formulation example. Through this, it can be confirmed that the mask pack containing the polar low molecular weight keratin peptide is excellent in increasing skin elasticity.

Experimental Example 5. Skin torsion and elasticity improvement effect human application experiment

After 44 adults were allowed to use Formulation Example and Comparative Formulation Example, skin torsion elasticity of the cheek area was measured before use, 2 weeks after use, and 4 weeks after use. The measured skin torsion elasticity improvement rate result values are summarized in Table 7 and FIG. 6 below.

Kinds 2 weeks after use 4 weeks after use Formulation example 6.7% 15.3% Comparative preparation example 1.2% 7.4%

As shown in the results of Table 7 and Figure 6, the formulation example containing the polar low molecular weight keratin peptide showed a higher improvement in skin torsion elasticity 2 weeks after use and 4 weeks after use than the comparative formulation example. Through this, it can be confirmed that the mask pack containing the polar low molecular weight keratin peptide has an excellent effect of increasing skin torsion elasticity.

Experimental Example 6. Skin moisture content improvement effect human application experiment

After 44 adults were allowed to use Formulation Example and Comparative Formulation Example, the amount of skin moisture in the cheek area was measured before use, 2 weeks after use, and 4 weeks after use. The measured skin moisture content improvement rate result values are summarized in Table 8 and FIG. 7 below.

Kinds 2 weeks after use 4 weeks after use Formulation example 16.1% 29.4% control formulation 8.4% 13.2%

As shown in the results of Table 8 and FIG. 7, the formulation example containing the polar low molecular weight keratin peptide showed a higher improvement in skin moisture content 2 weeks after use and 4 weeks after use than the comparative formulation example. Through this, it can be confirmed that the mask pack containing the polar low molecular weight keratin peptide has an excellent effect of increasing the moisture content of the skin.

Experimental Example 7. Skin color improvement effect human application experiment

After 44 adults were allowed to use the formulation example and the control formulation, the skin color of the cheek area was measured before use, 2 weeks after use, and 4 weeks after use. For skin color measurement, the L value (lightness) appearing on the skin surface was measured using a spectrophotometer (CM-2600d, Minolta, Japan), and the improvement rate (%) was calculated by comparing the measured values with those before use. The measured skin color improvement rate result values are summarized in Table 9 and FIG. 8 below.

Kinds 2 weeks after use 4 weeks after use Formulation example 5.7% 15.8% Comparative preparation example 3.1% 11.0%

As shown in the results of Table 9 and FIG. 8, the formulation example containing the polar low molecular weight keratin peptide showed a higher skin color improvement rate after 2 weeks and 4 weeks after use than the comparative formulation example. Through this, it can be confirmed that the mask pack containing the polar low molecular weight keratin peptide is excellent in improving skin color.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (9)

Contains a polar low molecular weight keratin peptide with a molecular weight of 10 kDa or less as an active ingredient,
The polar low molecular weight keratin peptide is a cosmetic composition for skin moisturizing or elasticity enhancement which is obtained by fractionating the keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent.
The method of claim 1,
The polar low molecular weight keratin peptide is a cosmetic composition for moisturizing or enhancing skin elasticity, characterized in that it is obtained by culturing Fervidobacterium islandicum AW-1.
3. The method of claim 2,
The Pervidobacterium islandicum AW-1 is a cosmetic composition for moisturizing or enhancing skin elasticity, characterized in that it is cultured in a medium containing chicken hair.
delete delete According to claim 1,
The polar low molecular weight keratin peptide is a cosmetic composition for moisturizing or enhancing skin elasticity, characterized in that it is included in an amount of 0.0001 to 100 wt% based on the total weight of the cosmetic composition.
According to claim 1,
The keratin peptide is characterized in that it exhibits any one activity selected from elastase inhibitory ability, procollagen type-1 C peptide (PIP) synthesis promoting ability, and α-elastin synthesis promoting ability with respect to fibroblasts. A cosmetic composition for moisturizing or enhancing skin elasticity.
According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it has a formulation selected from the group consisting of lotions, lotions, creams, essences, packs, emulsions, oil gels, serums, cleansing foams, makeup bases and foundations.
Fervidobacterium islandicum ( Fervidobacterium islandicum ) A first step of culturing AW-1 in a medium containing chicken hair to obtain a keratin peptide stock solution;
a second step of separating the polar keratin peptide mother liquid from the keratin peptide mother liquid using a mixture of a non-polar solvent and a polar solvent;
A third step of separating the polar low molecular weight keratin peptide mother liquid having a molecular weight of 10 kDa or less by passing the polar keratin peptide mother liquid through a membrane having a pore size of 10 kDa,
A method for producing a polar low molecular weight keratin peptide for skin moisturizing or elasticity enhancement.

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