KR101142010B1 - Cosmetic composition Comprising Moisture Quality Component As Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing, wrinkle improvement and elasticity containing MQC (Moisture Quality Component), the MQC is composed of low molecular weight hyaluronic acid or salts thereof, tricolor violet extract, trehalose, oligo alginate and ceramide. MQC of the present invention is excellent in the biological application effect is excellent in the moisturizing power of the skin, wrinkle improvement and elasticity enhancement effect when applying the skin can be usefully used as a cosmetic composition for the skin improvement effect.

Hyaluronic acid, tricolor violet, oligo alginate, ceramide, nano liposome

Description

Cosmetic composition containing MMC as an active ingredient {Cosmetic composition Comprising Moisture Quality Component As Active Ingredient}

The present invention relates to a cosmetic composition containing MQC (Moisture Quality Component) as an active ingredient, wherein the MQC is moisturizing skin, characterized in that it consists of low molecular weight hyaluronic acid or its salt, tricolor violet extract, trehalose, oligo alginate and ceramide It relates to a cosmetic composition for improving wrinkles and enhancing elasticity.

Skin is the site of direct physical and chemical injury from a variety of external factors. For example, the skin is damaged by various contaminants and stress. If the damage is not properly cell proliferation of the skin, the skin is even more damaged, the elasticity of the skin is quickly reduced, wrinkles are generated.

As the skin ages, the amount of collagen, mucopolysaccharides, and hyaluronic acid in the matrix, which play an important role in skin elasticity, decreases, and the amount of insoluble collagen increases. This makes it difficult for the dermis to keep the skin hydrated and the skin rough. In addition, the function of sebaceous glands and sweat glands is reduced, so the amount of sebum and sweat is gradually reduced. As a result, the sebaceous membrane is not properly formed and the protection against skin hydration is lost. As a result, the skin becomes dry and loses its translucency, thereby reducing the elasticity of skin proteins and producing wrinkles.

In addition, moisture in normal skin contains 70% in the body, 20% in the stratum corneum to support the elasticity and flexibility of the skin. If the moisture in the stratum corneum is less than 10%, the skin is known to dry out and cause skin problems. The amount of water in the stratum corneum produced by regular keratinization is always 10-20%.

Currently, the method of retaining moisture in the skin uses an occlusive agent, which is a hydrophobic substance that promotes water retention by forming a barrier on the skin that prevents water loss. The most commonly used occlusion agents include petrolatum, lanolin, cocoa butter, mineral oil and silicone, and cosmetic compositions according to the prior art include sodium lauryl sulfate and dimethyl sulfoxide as humectants. ), Sodium hydroxide, etc., generally contains 20 to 50 different raw materials.

However, these raw materials can be applied to products (cosmetics) to cause irritation such as skin irritation, itching and allergies. When the skin is exposed to irritating substances, the stratum corneum is damaged, thus increasing transepidermal water loss, which is an indicator of stratum corneum damage, which is also the cause of the primary inflammatory response.

As described above, the occlusion agent and the moisturizer contained in the conventional cosmetic composition has a problem that harmful side effects occur on the skin, for this reason there is a limit in obtaining a skin moisturizing effect, protective effect and the like.

Accordingly, research on using hyaluronic acid made of low molecular weight of 500-10,000 Daltons instead of the high molecular weight hyaluronic acid used in the past to enhance skin moisturizing power without causing side effects and make the skin healthy and beautiful, and aqua of the skin Increasing aquaporins have led to research into moisturizers through mechanisms that increase skin moisture absorption.

In humans, three billion water molecules per second pass through the skin, typically through a protein called aquaporin. Aquaporin is divided into several types according to structure and function. Among them, aquaporins-3, which is known to be related to skin, is present in keratinocytes and is known to exist in the basal layer of the epidermis.

On the other hand, in increasing the moisture retention amount of the epidermis, it is possible to promote the hydration of the skin to achieve. At this time, a molecule such as hyaluronic acid plays an important role in the skin hydration process. Hyaluronic acid in the epidermis also plays an important role in skin aging, the amount of hyaluronic acid is drastically reduced with age, wrinkles and aging occurs. That is, hyaluronic acid is produced in keratinocytes and plays an important role in hydration of the stratum corneum, as well as affects water retention and regulation of water and ion exchange.

In addition, by increasing the amount of hyaluronan synthase-2, which is an enzyme synthesizing hyaluronic acid, it is possible to promote the synthesis of hyaluronic acid.

In addition, by enhancing the activity of the CD44 receptor, which affects the ability of the produced hyaluronic acid to bind with water, it is possible to promote the synthesis of hyaluronic acid, and it is important to identify a component that promotes the synthesis of hyaluronic acid. Is being raised.

Hyaluronic acid is a linear polymer that combines N-acetylglucosamine and glucuronic acid, which are repeating units, as (1-3) and (1-4), and is a linear polymer for medical use in eye surgery, arthritis treatment, trauma coating, etc. For cosmetics, it has been mainly used as a moisturizer and skin protectant.

Recently, research on the function as a structure and a biologically active substance in human tissues, such as skin, in addition to the physical properties of hyaluronic acid has been actively conducted, and its use is also expanding from cosmetics and medicines to health functional foods.

Hyaluronic acid is widely present as an extracellular matrix component of tissues such as skin, cartilage, blood vessels, brain, and the like of humans and mammals, and is distributed throughout the body as it is contained in body fluids such as lymph and serum. Various types of hyaluronic acid binding proteins exist in vivo, and other matrix molecules bind to these binding molecules in tissues to form a large extracellular matrix. In addition to its role as a tissue construct, many recent studies have been conducted on the function of hyaluronic acid as a bioactive substance. The first is tissue-specific osmotic pressure, transparency, viscoelasticity, selective substance permeability, and extracellular matrix. The second is to participate in extracellular signal transduction by various forms of interaction with the hyaluronic acid binding protein.

Recent studies have shown that hyaluronic acid plays an important role in both intracellular and intracellular signaling as well as moisturizing and constructing.

On the other hand, the molecular weight of several hundreds of thousands of molecular weight hyaluronic acid has a disadvantage in that it is difficult to use at high concentrations due to its high viscosity, so that the concentration is limited in use in medicines such as cosmetics or injections.

On the other hand, low molecular weight hyaluronic acid having a molecular weight of tens of thousands to tens of thousands has high solubility compared to the conventional high molecular weight hyaluronic acid, so that it can be used in high concentration, has no stickiness, and has a polymerization degree of sugar of 10 to 14 It has been reported that the low molecular weight hyaluronic acid and its salts of sugars promote angiogenesis (slevin M, et al., J Biol Chem ., 25; 277 (43), pp41046-59, 2002).

However, in the present invention, the MQC including trehalose, oligo alginate and ceramide, including tricolor violet extract activating the low molecular weight hyaluronic acid and aquaporin, is far more moisturizing, wrinkle improvement and elasticity enhancement than other cosmetic ingredients known to date. The present invention was completed by demonstrating excellent effects, and in particular, when the MQC was included in nanoliposomes and stabilized, skin absorption was further increased to maximize the effect.

The present invention is MQM consisting of low molecular weight hyaluronic acid or its salt, tricolor violet extract, trehalose, oligo alginate and ceramide, having a weight average molecular weight of 500 to 30,000 daltons, which has an effect of improving wrinkles, moisturizing and elasticity of the skin. It aims at providing the cosmetics containing.

Another object of the present invention is to provide a cosmetic that can be maximized the effect of moisturizing, wrinkle improvement and enhancement of elasticity by using the MQC stabilized by the inclusion in nano liposomes stabilized in cosmetics.

Still another object of the present invention is to provide a cosmetic method of applying the cosmetic to human skin to impart a moisturizing, wrinkle improvement and elasticity enhancing effect.

The object of the present invention as described above is achieved by a cosmetic composition for improving skin moisturizing power, comprising as an active ingredient a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient.

Still another object of the present invention is achieved by a cosmetic composition for improving skin wrinkles, comprising as an active ingredient a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient.

Another object of the present invention is achieved by a cosmetic composition for enhancing skin elasticity, characterized by containing as an active ingredient MQC consisting of low molecular weight hyaluronic acid or its salt, tricolor violet extract, trehalose, oligo alginate and ceramide.

Preferably, the low molecular weight hyaluronic acid or its salt, tricolor violet extract, trehalose, oligo alginate and ceramide are mixed in a weight ratio of 0.0001-1.0: 0.0001-1.0: 0.0001-1.0: 0.0001-1.0: 0.0001-1.0 It is done.

Preferably, the MQC is characterized in that it comprises 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition.

In addition, the MQC is preferably characterized in that stabilized with nano liposomes consisting of oily components, polyols, surfactants, phospholipids, fatty acids and purified water.

In addition, preferably the low molecular weight hyaluronic acid or its salt is 0.001-10.0% by weight, tricolor violet extract is 0.001-10.0% by weight, trehalose is 0.01-20.0% by weight, 0.01-10.0 wt% alginate, 0.001-6.0 wt% ceramide, 2.0-40.0 wt% oily component, 10.0-80.0 wt% polyol, 0.1-10 wt% surfactant, 0.5-20.0 wt% phospholipid, The fatty acid is 0.05 to 3.0% by weight and purified water is characterized in that it comprises 1.0 to 40.0% by weight.

In addition, preferably, the nano-liposome stabilized MQC is characterized in that it comprises 0.01 to 50.0% by weight relative to the total weight of the cosmetic composition.

Also preferably, the cosmetic composition is a group consisting of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation and a spray It is characterized by having the formulation selected from.

Another object of the present invention is achieved by a cosmetic method characterized in that the cosmetic composition is applied to human skin to increase skin moisturizing power, promote biosynthesis of collagen, improve wrinkles, enhance elasticity or protect skin.

According to the present invention, a cosmetic composition containing MQC (Moisture Quality Component) consisting of a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient is effective in improving skin wrinkles, enhancing moisturizing power and enhancing elasticity. You can get it.

In addition, when the MQC is included in the nano liposomes and stabilized, it is easy to permeate the skin of the active ingredient can further maximize the skin wrinkle improvement, moisturizing and elasticity enhancing effect.

MQC contained as an active ingredient in the cosmetic composition of the present invention is a mixture of trehalose, oligo alginate, and ceramide with trehalose, oligo alginate, and ceramide, which is a mixture of trehalose, oligo alginate, and ceramide, which activate low-molecular-weight hyaluronic acid and aquaporin that can penetrate into the skin. Due to the combined use of the low molecular weight hyaluronic acid and aquaporin effect is further increased, and as a result, the cosmetic composition containing such an MQC as an active ingredient has excellent skin wrinkle improvement, moisturizing enhancement and light elasticity enhancing effect.

That is, the cosmetic composition containing the MQC of the present invention as an active ingredient improves collagen biosynthesis (Experimental Example 1), skin penetration effect (Experimental Example 2), moisturizing ability (Experimental Example 3), wrinkle improvement (Experimental Example 4), elasticity It has an improvement (Experimental Example 5).

The present invention relates to a cosmetic composition for moisturizing, improving wrinkles and enhancing elasticity containing MQC (Moisture Quality Component) consisting of low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient.

As used in the present invention, "MQC" is an abbreviation of 'Moisture Quality Component', which means 'moisture retention ingredient' in Korean, and low molecular weight hyaluronic acid or its salt, tricolor violet extract, trehalose, oligo alginate and ceramide. A mixture consisting of 'MQC' is defined.

The low molecular weight hyaluronic acid or salts thereof, tricolor violet extract, trehalose, oligo alginate and ceramide in the MQC are mixed in a weight ratio of 0.0001-1.0: 0.0001-1.0: 0.0001-1.0: 0.0001-1.0: 0.0001-1.0, preferably Is mixed in a weight ratio of 0.01-1.0: 0.01-1.0: 0.01-1.0: 0.01-1.0: 0.01-1.0, most preferably in a weight ratio of 0.5: 0.5: 1.0: 0.5: 0.3.

Among the components constituting the MQC of the present invention, the low molecular weight hyaluronic acid or its salt is highly efficient to be passed through the skin barrier and into the skin, thereby supplying hyaluronic acid directly into the skin, thereby decreasing the hyaluronic acid as the aging progresses. It is contained in the MQC of the present invention as a component to supplement the content of the extracellular matrix (Extracellular Matrix). In addition, by supplying hyaluronic acid directly into the cell, it prevents the decrease in elasticity of the skin and increases the water content, thereby exhibiting effects such as skin dryness and wrinkle improvement.

According to a preferred embodiment of the present invention, the low molecular weight hyaluronic acid or salt thereof has a weight average molecular weight of 500 to 30,000 Daltons, preferably 500 to 10,000 Daltons, more preferably 500 to 5,000 Daltons. If the weight average molecular weight is less than 500 Daltons, there is a problem that the moisturizing and wrinkle improvement effect is lowered because it does not maintain the same structure as hyaluronic acid, and if it exceeds 30,000 Daltons, the size of hyaluronic acid is large and it is not easy to be enclosed inside the nano liposome. However, there is a problem that penetration is difficult even when applied to the skin.

Among the components constituting the MQC of the present invention, the tricolor violet extract is contained as an active ingredient that promotes the synthesis of aquaporins-3 and hyaluronic acid related to the maintenance of moisture in the epidermis. In addition, the tricolor violet extract is involved in the binding of hyaluronic acid and is contained as an active ingredient that increases the synthesis of hyaluronan synthase-2 and the receptor CD44. Accordingly, the tricolor violet extract inhibits moisture evaporation of the epidermis and maintains moisture in the stratum corneum, thereby controlling the moisture content of the epidermis and improving the moisturizing ability and improving the wrinkles.

This tricolor violet ( Viola tricolor L. ) belongs to the family of violets and is an annual herb that is an improvement of the violet native to Northern Europe. Also called pansy or phalaenopsis. In the early 19th and early 20th centuries, it was improved in England and France to create the current garden pansy, 12 to 25 centimeters high, with stems growing straight or sideways, with many branches. The lower leaf is oval, and the upper leaf is long spatula. Petioles long, with large chin leaves. Flowers bloom from April to May, with long peduncles from the axillas, running one at the end. Flowers include small race, middle race and large race. The petals are five, but the shape is not the same, and the white, yellow, and purple three colors are different.

In the present invention, tricolor violet extract means that obtained by extracting from various organs or parts (such as leaves, flowers, roots, stems, branches, shells, and seeds) of tricolor violets, preferably leaves, flowers, stems Or an extract obtained from the seed, more preferably an extract obtained from a leaf or a flower, and most preferably an extract obtained from a flower.

The tricolor violet extract of the present invention may be prepared using a conventional solvent according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure, but preferably (a) water, 1 carbon 4 anhydrous or hydrous lower alcohols (e.g. methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, Solvent extraction using a solvent selected from the group consisting of chloroform, hexane and mixtures thereof (b) extraction using supercritical extraction by reduced pressure and high temperature with carbon dioxide or (c) ultrasonic extraction. In the present invention, a solvent extraction method using an extraction solvent is preferably used.

Tricolor violet extract in the present invention is a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), the mixed solvent of the lower alcohol and water, acetone, Ethyl acetate, chloroform, 1,3-butylene glycol, and butyl acetate can be obtained from the flowers of the tricolor violet using extraction solvent. More preferably, the extract of the present invention is obtained using a hydrous lower alcohol, preferably an aqueous ethanol solution, and most preferably a 70% (v / v) aqueous ethanol solution.

On the other hand, it will be apparent to those skilled in the art that the tricolor violet extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

In addition, the tricolor violet extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention. Extracts of the present invention can be prepared by additional procedures such as vacuum concentration. In addition, the extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

Among the components constituting the MQC of the present invention, trehalose, oligoalginate and ceramide hydrate the skin, improve skin texture and help maintain healthy skin, and the low molecular weight hyaluronic acid or its salt and tricolor violet It is an antagonistic component which makes the above-mentioned action effect of an extract more remarkable.

That is, the low molecular weight hyaluronic acid or its salt and tricolor violet extract in the MQC of the present invention are used in combination with trehalose, oligoalginate and ceramide, so that the effect is lower than that when only the low molecular weight hyaluronic acid or its salt and tricolor violet extract is used. Much more.

As such, the augmentation effect obtained by mixing low molecular weight hyaluronic acid or its salt and tricolor violet extract with trehalose, oligoalginate and ceramide may be achieved by the group consisting of trehalose, oligoalginate and ceramide to low molecular weight hyaluronic acid or its salt and tricolor violet extract. It can also be confirmed in contrast with the effect of the mixture with one or two components selected from.

The content of MQC (low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate and ceramide mixture) in the present invention is 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition, more preferably 0.01 to 20.0% by weight to be. If the content of MQC is less than 0.001% by weight, there is a problem that the content of the effective substance is too small to achieve the desired effect, and when the content of 30.0% by weight is exceeded, it is difficult to make a normal formulation.

According to the cosmetic composition of the present invention containing MQC as an active ingredient, skin moisturizing, skin wrinkle improvement and elasticity effect can be obtained.

More preferably, when the MQC is stabilized and used with nano liposomes, skin permeation of the active ingredient may be facilitated, thereby maximizing skin protection effects such as skin wrinkle improvement, moisturizing and elasticity enhancing effects.

As used herein, the term "nano liposome" is in the form of liposomes used for the stable delivery of physiologically active ingredients in the pharmaceutical, cosmetic and food fields, and to maximize the penetration effect, the average particle diameter of 1 ~ 500 nm ( Nanometers).

According to a preferred embodiment of the present invention, the average particle diameter of the nano liposomes is preferably 30 to 100 nm. When the average particle diameter of the nano liposome exceeds 100 nm, the improvement of skin penetration and the improvement of formulation stability may be very weak among the technical effects to be achieved in the present invention.

The nano liposomes may be prepared by a mixture comprising an oily component, a polyol, a surfactant, a phospholipid, a fatty acid, and purified water, and it is preferable that no monohydric alcohol (eg, ethanol) is used in the mixture.

The oil component used in the preparation of the nano liposome of the present invention may be various oils known in the art, for example, hydrocarbon-based oils such as hexadecane and paraffin oil, isopropyl myristate and Synthetic oils of the same ester type, silicone oils such as dimethicone and cyclomethicone type, animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil, jojoba oil, nut oil, squalane and fish oil. Ethoxylated alkylether based oils, propoxylated alkylether based oils, sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine, cerebrose, cholesterol, cytososterol, cholesteryl sulfate, cytosine _{Sterylsulfate} , C _10-40 fatty alcohol or ceramide. Preferably, hydrocarbon oils such as hexadecane and paraffin oil, silicone oils such as dimethicone and cyclomethicone, or sunflower oil, corn oil, soybean oil, avocado oil, sesame oil, jojoba oil, nut oil, squalane and It is a plant and animal oil such as fish oil. Preferred among hydrocarbon oils are paraffin oils, preferred isopropyl myristate among ester synthetic oils, dimethicone is preferred among silicone oils, and nut oils and squalanes are preferred among animal and vegetable oils. More preferably, it is paraffin oil, isopropyl myristate, dimethicone, squalane or nut oil, most preferably a mixture of the five oils.

The polyol used in the preparation of the nano liposome of the present invention is not particularly limited, and for example, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isoprene glycol, pentylene glycol, erythritol, It is possible to use one or more selected from the group consisting of ziitol and sorbitol, preferably propylene glycol.

In addition, the surfactant used in the preparation of the nano liposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants May be used, preferably anionic surfactants and nonionic surfactants are used. Specific examples of the anionic surfactant include alkylacyl glutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate. Specific examples of the nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters, and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has an amount of 23 to 95% of phosphatidyl choline and 20% or less of phosphadidylethanolamine.

The fatty acids used in the preparation of the nano liposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of a C _{12 -22} alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid or linoleic acid. It includes.

The water used to prepare the nano liposomes of the present invention is generally deionized distilled water.

The preparation of the nano liposomes can be made through various methods, but most preferably, the nano liposome constituents and low molecular weight hyaluronic acid or its salts, tricolor violet extract, trehalose and oligo alginate are applied to an ultrahigh pressure homogenizer. .

0.001 to 10.0% by weight, preferably 0.01 to 8.0% by weight, tri-color violet extract 0.001 to 10.0% by weight, preferably 0.01 to 8.0, based on the total weight of MQC stabilized with nano liposomes % By weight, trehalose 0.01 to 20.0% by weight, preferably 0.1 to 15.0% by weight, oligo alginate 0.01 to 10.0% by weight, preferably 0.1 to 8.0% by weight, ceramide 0.001 to 6.0% by weight, preferably 0.01 to 5.0% by weight %, Oil component 2.0 to 40.0 wt%, preferably 3.0 to 20.0 wt%, phospholipid 0.5 to 20.0 wt%, preferably 2.0 to 8.0 wt%, polyol 10.0 to 80.0 wt%, preferably 30.0 to 70.0 wt% , 0.1 to 10.0% by weight of surfactant, preferably 0.5 to 5.0% by weight, 0.05 to 3.0% by weight of fatty acid, preferably 0.1 to 1.0% by weight, 1.0 to 40.0% by weight of purified water, preferably It is prepared by applying a ultra-high pressure homogenizer and mixed in an amount of from 2.0 to 30.0% by weight.

Preparation of the nano liposomes by the high pressure homogenizer has an advantage that can be carried out under various conditions (eg, pressure, frequency, etc.) according to the desired particle size. In this case, preferably, the nano liposome may be prepared by passing the high-pressure homogenizer 1 to 5 times under a pressure of 600 to 1200 bar.

The nano liposomes prepared as above have the advantage of maximizing the skin penetration effect by stabilizing unstable substances and dissolving several kinds of poorly soluble substances.

The content of MQC (a mixture of low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate and ceramide) stabilized by inclusion in nano liposomes is 0.001-50.0 wt% with respect to the total weight of the cosmetic composition, more preferably 0.1 25.0% by weight.

Further, preferably, the content of MQC in the stabilized MQC included in the nano liposomes is 0.001-50.0% by weight, more preferably 0.01-40.0% by weight relative to the total weight of the MQCs stabilized with the nano liposomes. .

On the other hand, the cosmetic composition according to the present invention includes components conventionally used in cosmetic compositions in addition to MQC and / or nanoliposomes stabilized with low molecular weight hyaluronic acid or salts thereof, tricolor violet extract, trehalose, oligo alginate and ceramide Such as conventional stabilizers, solubilizers, vitamins, pigments and flavorings, and carriers.

In addition, the cosmetic composition according to the present invention may be prepared in the form of a general formulation in the art, for example, an emulsion formulation or a solubilized formulation. Examples of the emulsified formulations include nutrient cosmetics, creams, essences, and the like. Examples of solubilized formulations include softening cosmetics. In addition, the cosmetic composition of the present invention may be prepared in the form of adjuvants that can be applied topically or systemically, which is commonly used in the field of dermatology by containing a dermatologically acceptable medium or base in addition to cosmetics.

Suitable formulations of cosmetics include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the MQC (low molecular weight hyaluronic acid or salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide) and / or MQC stabilized with nano liposomes of the present invention, fatty substances, organic solvents, Solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, blowing agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, It may contain adjuvants commonly used in the cosmetic or dermatological field, such as blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, etc. Etc.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream , Nourishing cream, moisturizing cream, hand cream, essence, nourishing essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, eye shadow, patch, spray It may be prepared in a formulation such as.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The present invention also provides a cosmetic method comprising applying a cosmetic composition containing MQC stabilized with MQC or nano liposomes as an active ingredient to human skin to have skin moisturizing, wrinkle improvement and elasticity enhancing effects.

The cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all the methods known in the art using the cosmetic composition belong to the makeup method of the present invention.

The cosmetic composition of the present invention may be used in a single or overlapping application, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic method using the cosmetic composition containing the MQC of the present invention as an active ingredient is carried out, it is possible to obtain effects such as skin moisturizing, wrinkle improvement and elasticity enhancement, and a cosmetic containing MQC stabilized with nano liposomes as an active ingredient. When the composition is used, the skin absorption and penetration effect are further increased, thereby maximizing the effects of skin moisturizing, wrinkle improvement and elasticity enhancement, and much better skin permeability can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Experimental Examples in order to help the understanding of the present invention. However, the following preparation examples and demonstration examples are provided only to explain the present invention in more detail, whereby it will be apparent to those skilled in the art that the technical scope of the present invention is not limited. .

Preparation Example 1 Preparation of Tricolor Violet Extract

Tricolor violet flowers were washed with purified water, dried, peeled and finely powdered. To 100 g of powdered trichromatic violet powder, 70% aqueous alcohol solution was added in an amount of 1-15 times, and then immersed for 3 days, filtered through Whatman filter paper, and the filtrate was concentrated in vacuo to obtain 3.5 g of dry extract. The dry extract was dissolved in 50% 1,3-butylene glycol solution to a concentration of 1% (w / v), filtered and used for the experiment of the present invention.

Preparation Example 2 Preparation of MQC (Moisture Quality Component)

Low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate, ceramide and glycerin were respectively mixed 16.67 wt%: 16.67 wt%: 33.33 wt%: 16.67 wt%: 10.0 wt%: 6.67 wt% and then 70-77 ° C The mixture was heated and mixed until it was used in the experiment of the present invention by stirring uniformly.

Preparation Example 3: Preparation of MQC (Moisture Quality Component) stabilized with nano liposomes

To prepare a nano liposome with the composition and content of Table 1 below, to the composition of the following Table 1, B phase (lecithin) is first added to phase A (polyol), and then uniformly wetted, and then the C phase A, B Phase, phase D were added and stirred uniformly after heating and mixing to 70-77 ° C.

Subsequently, the mixture is passed through a high-pressure homogenizer 1-5 times under conditions of 600-1,200 bar, and then cooled to cool low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate, and ceramide having a particle size of 30 to 100 nm. MQC consisting of the nano-liposomes are included to prepare a stabilized form of MQC.

Manufacturing of MQC (Moisture Quality Component) Prize ingredient Content (% by weight) A glycerin to 100 B Hydrogenated lecithin 8.0 C Ceramide 3.0 Macadamia Nut Oil 1.0 Squalane 1.0 Dimethicone 1.0 Isopropyl myristate 1.0 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 D Low molecular weight hyaluronic acid
(Weight average molecular weight: 1,000) 5.0 Tricolor Violet Extract 5.0 Trehalose 10.0 Oligo Alginate 5.0 Potassium hydroxide Quantity Purified water 5.0

Preparation Example 4 Preparation of Cosmetic Composition Containing MQC or MQC Stabilized with Nano Liposomes

Cosmetic composition containing MQC consisting of active ingredients of the present invention, low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate and ceramide as active ingredients, and MQC stabilized by nanoliposomal stabilization, that is, MQC stabilized with nanoliposomes. A cosmetic composition containing was prepared as an active ingredient.

MQCs (low molecular weight hyaluronic acid, tricolor violet extract, trehalose, oligo alginate and ceramide) were used in Preparation Example 2, and MQCs stabilized with nano liposomes were used in Preparation Example 3.

Cosmetic compositions containing MQC stabilized with MQC or nano liposomes were prepared with the ingredients and contents shown in Table 2 below.

Cosmetic composition containing MQC (Preparation Example 3) stabilized with nano-liposomes of the present invention is called Example 1, cosmetic composition containing MQC (Preparation Example 2) Example 2, cosmetics containing only low molecular weight hyaluronic acid Comparative Example 1, cosmetic composition comprising only tricolor violet extract Comparative Example 2, cosmetic composition comprising low molecular weight hyaluronic acid and tricolor violet extract Comparative Example 3, low molecular weight hyaluronic acid, tricolor violet extract, trehalose and oligo alginate Cosmetic composition comprising a comparative composition 4, low molecular weight hyaluronic acid, tricolor violet extract, trehalose and ceramide cosmetic composition comprising a comparative example 5, low molecular weight hyaluronic acid, tricolor violet extract, oligo alginate and ceramide Comparative Example 6, cosmetics containing only nano liposomes except MQC It was referred to as Comparative Example 7.

The A and B phases were each warmly mixed at 75 ° C., emulsified, cooled to 40 ° C., and the C phase was added and mixed to form a final product. In Table 2, the content unit of the cosmetic composition is wt%.

Preparation of cosmetic composition comprising MQC stabilized with MQC or nano liposomes ingredient Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 content content content content content content content content content A Glyceryl
Stearate 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Cetearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Polysorbate 60 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan
Sesquioleate 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Fiji-100 Stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Squalane 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Caprylic / capric triglyceride 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Ceramide - - - - - - 0.73 0.93 - B glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Xanthan Gum 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Incense, preservative Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water Balance Balance Balance Balance Balance Balance Balance Balance Balance C With nano liposomes
Stabilized MQC (Manufacturing Example 3) 20.0 - - - - - - - - MQC
(Manufacture example 2) - 5.6 - - - - - - - Low molecular weight hyaluronic acid - - 5.6 - 2.8 1.12 1.22 1.56 - Tricolor Violet Extract - - - 5.6 2.8 1.12 1.22 1.56 - Trehalose - - - - - 2.24 2.43 - - Oligo Alginate - - - - - 1.12 - 1.56 - Nano liposomes except MQC - - - - - - - - 20.0

Experimental Example 1: MQC Experimental study on collagen biosynthesis

Human normal fibroblasts were seeded to 1 x 10 ⁶ cells in each well of a 48-well microplate and incubated for 24 hours at 37 ° C. in Dulbecco's Modified Eagle Medium (DMEM) medium. Subsequently, the experimental group in which low-molecular-weight hyaluronic acid and tricolor violet extract, which are active substances constituting MQC, were replaced with DMEM medium without serum with 1.0 and 2.0%, and serum-free DMEM without low-molecular weight hyaluronic acid and tricolor violet extract Controls replaced with medium were further incubated for 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a procollagen type IC-peptide (PICP) kit (Takara, Japan). The amount of PICP was converted to ng / ml, and ascorbic acid was used as a positive control. Collagen biosynthesis increase rate was calculated by the following equation 1 and the results are shown in Table 3.

Collagen Biosynthesis Enhancement Effect of MQC Constituents Test substance concentration (%) Collagen Biosynthesis Growth Rate (%) Positive control (ascorbic aicd 250 uM) 67.5 Low molecular weight hyaluronic acid 1.0% 32.8 Low molecular weight hyaluronic acid 2.0% 47.5 Tricolor Violet Extract 1.0% 33.8 Tricolor Violet Extract 2.0% 48.2 Low molecular weight hyaluronic acid 0.5%
+ Tricolor violet extract 0.5% 41.7 Low molecular weight hyaluronic acid 1.0%
+ Tricolor Violet Extract 1.0% 60.8 Low molecular weight hyaluronic acid (0.4)%
Tricolor Violet Extract (0.4)%
+ Trehalose (0.8)%
Increased and alginate (0.4)% 59.3 Low molecular weight hyaluronic acid (0.43)%
Tricolor Violet Extract (0.43)%
+ Trehalose (0.88)%
+ Ceramide (0.26)% 61.7 Low molecular weight hyaluronic acid (0.56)%
Tricolor Violet Extract (0.56)%
+ Oligo-alginate (0.56)%
+ Ceramide (0.32)% 32.4 Low molecular weight hyaluronic acid (0.36)%
Tricolor Violet Extract (0.36)%
+ Trehalose (0.71)%
+ Oligo-alginate (0.36)%
+ Ceramide (0.21)% 32.6

According to Table 3, it can be seen that the low molecular weight hyaluronic acid and tricolor violet extract have an excellent effect on promoting collagen biosynthesis. In addition to low-molecular-weight hyaluronic acid and tricolor violet extract, trehalose, oligo alginate, and ceramide were used together to find the best effect.

Experimental Example 2: Experiment of skin penetration of MQC stabilized with nano liposomes

The amount of drug released from Examples 1 and 2 and Comparative Examples 1 and 2 was determined using a Modified Franz diffusion cell. Examples 1 and 2 and Comparative Examples 1 and 2 were applied to 4 cm 2 of extracted skin from rats, and the extracted skin was fixed between the donor and receptor phases. PH 7.4 phosphate buffer prepared by the above was used. The volume of the cell was maintained at 11 ml, the temperature during the experiment was 37 ± 0.5 ℃, the stirring speed was 600 rpm. After 4 hours, 1 mL of each sample was accurately collected using a micro pipette, and the concentration of low molecular weight hyaluronic acid was analyzed by GPC (gel permeation chromatography), and the concentration of tricolor violet extract was measured using HPLC.

Gel permeation chromatography (GPC) was performed using a VP-10AD, RID-10A (shimadzu, Japan) instrument, and columns were Ultrahydrogel ™ 120, 250 (7.8 × 300 mm, WATERS, USA). As the eluate, 0.1 M sodium nitrate solution was used, and the flow rate was 0.5 ml / min. Ethylene glycol polymer was used as a standard material for molecular weight analysis. The results are shown in Tables 4 and 5 below.

Skin Penetration Effect of MQC Active ingredient Cell Permeability (%) Standard Deviation Example 1 Low molecular weight hyaluronic acid 52.32 0.78 Tricolor Violet Extract 56.69 0.87 Example 2 Low molecular weight hyaluronic acid 32.65 0.82 Tricolor Violet Extract 12.32 0.94 Comparative Example 1 Low molecular weight hyaluronic acid 23.37 0.6 Tricolor Violet Extract - - Comparative Example 2 Low molecular weight hyaluronic acid - - Tricolor Violet Extract 6.98 0.85

As shown in Table 4 and Table 5, Example 1 containing MQC stabilized with nano liposomes and Example 2 containing MQC in Comparative Examples 1 and 2 using a low molecular weight hyaluronic acid and tricolor violet extract alone Compared with the low molecular weight hyaluronic acid more than two times, tricolor violet extract was found to have an excellent skin penetration effect of about 7.7 times. This is because the average particle size of the nano liposome is very small to 30 ~ 100nm it is believed that the skin penetration is easy. In addition, as a result of evaluating the formulation stability, the low molecular weight hyaluronic acid and tricolor violet extract in the form of nano liposomes were stabilized by MQC (Example 1) and MQC (Example 2) is 45 ℃, 4 ℃ and -4 ℃ ~ 40 After 12 weeks of storage in the Cycling state of ℃ was confirmed to exist in a very stable state.

Experimental Example 3: Experiment to improve the skin moisturizing effect of MQC stabilized with MQC and nano liposomes

To determine the effect of improving the skin moisturizing power of the cosmetic composition containing MQC stabilized with a cosmetic or nano-liposomes containing MQC of the present invention was measured as follows.

Thirty women aged 35 to 53 years (average age 43 years) were subjected to four weeks application (two times / day) of the cosmetic composition prepared according to Table 2 of Preparation Example 4 as a subject. The moisture level of the epidermal layer directly below the stratum corneum was measured by measuring the electrostatic load capacity of the skin according to the moisture content using a corneometer (Corneometer CM825, Courage & Khazaka, Germany). The results are shown in Table 6 and Table 7.

MQC's skin moisturizing effect-1 Subject Example 1 Example 2 Comparative Example 1 Comparative Example 2 D0 D28 D0 D28 D0 D28 D0 D28 One 37 62 39 51 38 43 40 45 2 42 65 41 53 40 46 43 49 3 53 80 54 70 57 65 56 64 4 28 63 27 43 28 32 29 33 5 41 64 43 56 43 49 43 49 6 51 77 53 69 52 59 54 61 7 43 65 45 59 44 50 44 50 8 25 63 26 44 24 27 26 30 9 39 66 42 55 41 47 43 49 10 47 71 49 64 50 57 51 58 11 43 65 42 55 45 51 40 45 12 53 80 51 66 54 62 50 57 13 31 63 30 48 32 36 31 35 14 43 65 44 57 42 48 43 49 15 51 77 50 65 52 59 49 56 16 35 62 34 47 32 36 36 41 17 54 81 53 69 52 59 56 64 18 36 66 37 48 34 39 36 43 19 27 67 25 42 26 30 27 31 20 45 68 43 56 41 47 43 49 21 42 63 45 59 43 49 44 50 22 25 62 26 43 24 27 27 31 23 43 65 42 55 40 46 43 49 24 52 78 51 66 56 64 53 60 25 47 71 45 59 49 56 42 46 26 54 81 52 68 54 62 53 60 27 34 63 36 47 35 40 33 37 28 47 71 46 60 48 55 46 52 29 28 63 26 41 27 31 25 28 30 53 80 52 68 53 60 50 57 Average 41.63 68.68 41.63 55.98 41.87 47.73 41.93 47.59 Δ (D28-D0) 27.05 14.35 5.86 5.66

MQC's skin moisturizing effect -2 Subject Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 D0 D28 D0 D28 D0 D28 D0 D28 D0 D28 One 39 44 49 56 37 42 38 43 38 40 2 51 58 38 43 39 44 44 49 42 44 3 49 56 53 61 55 62 49 55 54 57 4 32 36 32 37 31 35 29 32 27 28 5 61 69 49 56 40 45 45 50 42 44 6 45 51 51 58 51 58 56 63 53 56 7 39 44 43 49 43 49 43 48 43 45 8 43 49 28 32 27 31 25 28 26 27 9 51 58 43 49 46 52 44 49 42 44 10 53 60 49 56 45 51 47 53 49 51 11 29 33 51 58 49 55 52 58 43 45 12 51 58 28 32 46 52 52 58 52 55 13 32 36 33 38 32 36 35 39 32 34 14 42 48 45 51 46 52 39 44 42 44 15 51 58 51 58 45 51 49 55 50 53 16 53 60 34 39 39 44 31 35 34 36 17 34 39 52 59 54 61 49 55 53 56 18 39 44 34 39 36 41 30 34 33 35 19 26 30 26 30 29 33 31 35 28 29 20 46 52 42 48 48 54 51 57 46 48 21 43 49 46 53 36 41 41 46 42 44 22 25 28 27 31 29 33 24 27 24 25 23 41 47 42 48 41 46 40 45 42 44 24 56 64 56 64 49 55 53 59 51 54 25 45 51 49 56 58 66 47 53 49 51 26 52 59 54 62 53 60 49 55 56 59 27 35 40 35 40 34 38 38 43 35 37 28 45 51 48 55 42 47 45 50 47 49 29 27 31 27 31 32 36 33 37 25 26 30 49 56 53 61 49 55 54 60 52 55 Average 42.80 48.58 42.27 48.27 42.03 47.50 42.10 47.15 41.73 43.82 Δ (D28-D0) 5.78 6.00 5.46 5.05 2.09

As shown in Table 6 and Table 7, when looking through the relative numerical calculation before and after the application of Examples 1, 2 and Comparative Examples 1 to 7, the results of the coneometer measurement to specify the moisture level of the skin does not include nano liposomes In the case of Example 2 containing only the MQC did not increase the water content was 14.35% after 28 days of application, it was confirmed that Example 1 containing the MQC stabilized with nano liposomes increased by 27.05%. This effect was observed in most subjects.

Therefore, through the present experiment, it was found that the cosmetics containing only MQC and MQC stabilized with nano liposomes (Examples 1 and 2) have an excellent effect on skin moisturizing ability by enhancing skin moisture content. In addition, the cosmetic composition containing the MQC stabilized with nano liposomes (Example 1) has a high skin penetration, compared to Example 2, which applied only MQC only, Comparative Example 1, applying only the low molecular weight hyaluronic acid, Comparative Example applying only tricolor violet extract 2, Comparative Example 3 applying low molecular weight hyaluronic acid and tricolor violet extract, Comparative Example 4 applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and oligo alginate, comparison applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and ceramide Example 5, low molecular weight hyaluronic acid, tricolor violet extract, oligo alginate and ceramides about 5 times compared to Comparative Example 6, except for MQC showed about 14 times higher moisturizing effect compared to Comparative Example 7 applying only nano liposomes And it was found.

Experimental Example 4: Experiment of wrinkle improvement effect of cosmetic composition containing MQC stabilized with MQC or nano liposome

Wrinkle improvement effect of the cosmetic of the present invention was evaluated through a practical use test. The cosmetic composition according to Table 2 of Preparation Example 4 was used, and in Example 1, a cosmetic composition in which MQC stabilized with nanoliposomes was replaced with purified water was used as a control example. The degree of wrinkle improvement was confirmed by visually evaluating the wrinkle improvement effect after 8 weeks using both sides of the face of 60 women. The wrinkle improvement effect results based on this evaluation are shown in Table 8 below.

Wrinkle improvement effect of cosmetic composition containing MQC stabilized with MQC or nanoliposomes Cosmetics Wrinkle improvement Effectiveness (%) Great slightly none Example 1 17 2 One 95.0 Example 2 9 4 7 65.0 Comparative Example 1 3 4 13 35.0 Comparative Example 2 4 2 14 30.0 Comparative Example 3 3 4 13 35.0 Comparative Example 4 One 4 15 25.0 Comparative Example 5 4 2 14 30.0 Comparative Example 6 3 4 13 35.0 Comparative Example 7 3 4 13 35.0 Control Example One 2 17 15.0

As shown in Table 8, looking at the wrinkle improvement effect of Examples 1 and 2 and Comparative Examples 1 to 7, Example 2 containing only MQC not included in nano liposomes was effective 65.0% after 8 weeks of application , Example 1 containing MQC stabilized with nano liposomes was confirmed that the effective rate is 95.0%.

Therefore, through the present experiment, it was found that the cosmetics containing only MQC and MQC stabilized with nano liposomes (Examples 1 and 2) exhibited an excellent skin wrinkle improvement effect. In addition, the cosmetic composition comprising the MQC stabilized with nano liposomes (Example 1) has a high skin penetration, compared to Example 2, which applied only MQC only, Comparative Example 1, applying only low molecular weight hyaluronic acid, Comparative Example applying only tricolor violet extract 2, Comparative Example 3 applying low molecular weight hyaluronic acid and tricolor violet extract, Comparative Example 4 applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and oligo alginate, comparison applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and ceramide Example 5, about 63% higher than that of Comparative Example 6 applying low molecular weight hyaluronic acid, tricolor violet extract, oligo alginate and ceramide, and about 80% higher effective than Comparative Example 7 applying nanoliposome only except MQC Could know.

Experimental Example 5: Experiment to improve the elasticity of the cosmetic composition containing MQC stabilized by MQC or nano liposomes

The skin elasticity improving effect of the cosmetic containing MQC stabilized with MQC and nano liposome of the present invention was evaluated through a practical test. It evaluated using the cosmetic composition prepared according to Table 2 of Preparation Example 4. Thirty 35-53 year old women (mean age 53 years) were randomly divided into 2 groups, and the cosmetics of Example 1 and Comparative Example 4 were washed twice daily each morning / evening, followed by an appropriate amount for 8 consecutive weeks based on both cheeks. Correctly The skin elasticity improvement effect of each subject was evaluated by using a cutometer (Cutometer MPA 580, Courage & Khazaka, Germany). The experimental results are shown in Table 9 below.

Elasticity improvement effect of cosmetic composition containing MQC stabilized with MQC or nanoliposomes Cosmetics Elasticity enhancing effect Effectiveness (%) Great slightly none Example 1 14 11 5 83.3 Example 2 10 8 12 60.0 Comparative Example 1 4 2 24 20.0 Comparative Example 2 3 5 22 26.7 Comparative Example 3 5 3 22 26.7 Comparative Example 4 6 4 20 33.3 Comparative Example 5 4 2 24 20.0 Comparative Example 6 4 5 20 33.3 Comparative Example 7 3 4 23 23.3

As shown in Table 9, looking at the elasticity improvement effect of Examples 1 and 2 and Comparative Examples 1 to 7, Example 2 containing only MQC not included in nano liposomes was effective 60.0% after 8 weeks of application , Example 1 containing MQC stabilized with nano liposomes was confirmed that the effective rate is 83.3.0%.

Therefore, it was found that the cosmetics containing only MQC and MQC stabilized with nano liposomes (Examples 1 and 2) exhibited an excellent skin elasticity improving effect through this experiment. In addition, the cosmetic composition comprising the MQC stabilized with nano liposomes (Example 1) has a high skin penetration, compared to Example 2, which applied only MQC only, Comparative Example 1, applying only the low molecular weight hyaluronic acid, Comparative Example applying only tricolor violet extract 2, Comparative Example 3 applying low molecular weight hyaluronic acid and tricolor violet extract, Comparative Example 4 applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and oligo alginate, comparison applying low molecular weight hyaluronic acid, tricolor violet extract, trehalose and ceramide Example 5, low molecular weight hyaluronic acid, tricolor violet extract, oligo alginate and ceramides about 57% compared to Comparative Example 6, except for MQC showed about 60% higher effective than Comparative Example 7 applying only nano liposomes Could know.

Moreover, no skin irritation could be observed in the subjects of the part which apply | coated the cosmetic of this invention to skin.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (10)

A cosmetic composition for enhancing skin moisturizing power, comprising a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient. A cosmetic composition for improving skin wrinkles, comprising a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient. A cosmetic composition for enhancing skin elasticity, comprising a low molecular weight hyaluronic acid or a salt thereof, tricolor violet extract, trehalose, oligo alginate and ceramide as an active ingredient. The method according to any one of claims 1 to 3, wherein the active ingredient is a low molecular weight hyaluronic acid or its salt: tricolor violet extract: trehalose: oligo alginate: ceramide = 0.0001-1.0: 0.0001-1.0: 0.0001-1.0: 0.0001 -1.0: Cosmetic composition characterized in that it is mixed by the weight ratio of 0.0001-1.0. The cosmetic composition according to any one of claims 1 to 3, wherein the moisturizing maintenance component (MQC) is contained in an amount of 0.001 to 30.0 wt% based on the total weight of the cosmetic composition. The method of claim 1, wherein the moisture quality component (MQC) is stabilized with nano liposomes consisting of an oil component, a polyol, a surfactant, a phospholipid, a fatty acid, and purified water. Cosmetic composition. The method according to claim 6, wherein the low molecular weight hyaluronic acid or its salt is 0.001-10.0% by weight, and the tricolor violet extract is 0.001-10.0% by weight, based on the total weight of the moisturizing maintenance component (MQC) stabilized with nano liposomes. , 0.01-20.0 wt% of trehalose, 0.01-10.0 wt% of oligo alginate, 0.001-6.0 wt% of ceramide, 2.0-40.0 wt% of oily component, 10.0-80.0 wt% of polyol, 0.1-10 wt% of surfactant %, Phospholipid is 0.5-20.0% by weight, fatty acid is 0.05-3.0% by weight and purified water is characterized in that it comprises 1.0 to 40.0% by weight. The cosmetic composition of claim 6, wherein the moisturizing maintenance component (MQC) stabilized with nano liposomes is included in an amount of 0.001 to 50.0 wt% based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion Cosmetic composition, characterized in that it has a formulation selected from the group consisting of foundation, wax foundation and spray. A cosmetic method according to any one of claims 1 to 3, wherein the cosmetic composition is applied to human skin to increase skin moisturization, promote biosynthesis of collagen, improve wrinkles, enhance elasticity or protect the skin.