KR20070121338A - A cosmetic composition regulating of the process of epidermal hydration containing a extract of viola tricolor l - Google Patents

Abstract

A cosmetic composition comprising the extract of Viola tricolor L. is provided to inhibit water evaporation from the skin and hold water in a horny layer in the skin without side effects by promoting synthesis of aquaporine-3 and increasing synthesis of receptors related to hyaluronic acid and its binding. A cosmetic composition for regulating the epidermal hydration, moisturizing the skin and improving skin wrinkles comprises 0.005-50 wt.% of the extract of Viola tricolor L. which is prepared by extracting with water, C1-C4 lower alcohol or a mixed solvent thereof, and is formulated as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, powder or eye shadow.

Description

A cosmetic composition regulating of the process of epidermal hydration containing a extract of Viola tricolor L.}

The present invention relates to an epidermal moisture control composition containing Pansy (Viola tricolor L.) extract.

Skin is an indispensable tissue in our lives, a site that is directly affected by physical and chemical injuries from various external factors, as well as having the ability to cure their functional and structural damage. However, our skin is damaged by various contaminants and stresses, for example, and when the cell's cell proliferation is not performed properly, the skin is damaged more and more quickly, , Wrinkles are created. As the skin ages, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the matrix, which play an important role in skin elasticity, decreases, and the amount of insoluble collagen increases. This makes it difficult for the dermis to keep the skin hydrated and the skin rough. In addition, the function of the sebaceous glands and sweat glands is also reduced, and the amount of sebum and sweat is gradually reduced. As a result, the sebaceous membrane is not properly formed, and the protection against skin hydration is lost. As a result, the skin becomes dry and translucency is lost, thereby reducing the elasticity of the skin protein and generating wrinkles. On the other hand, if the skin's moisturizing function does not function, it can cause skin elasticity. In other words, the loss of moisturizing skin is an important factor of skin aging. Moisture balance in the skin is controlled by the epidermis. The stratum corneum of the epidermis is the accumulation of dead cells, but plays a very important role in maintaining skin moisture. Human living bodies are mostly composed of water, which plays an important role in maintaining life. Moisture in normal skin is 70% in the body and 20% in the stratum corneum to support the elasticity and flexibility of the skin. If the moisture in the stratum corneum is less than 10%, the skin is known to dry out and cause skin problems. The amount of water in the stratum corneum produced by regular keratinization is always 10-20%. Currently, the method of retaining moisture in the skin uses an occlusive agent, which is a hydrophobic substance that promotes water retention by forming a barrier on the skin that prevents water loss. The most commonly used occlusion agents include petrolatum, lanolin, cocoa butter, mineral oil and silicone. In addition, to make cosmetics, 20 to 50 kinds of various raw materials are generally used such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, sodium hydroxide, and the like. Raw materials can be applied to products, which can cause irritation, itching and allergies to the skin. When the skin is exposed to irritating substances, the stratum corneum is damaged, thus increasing the transepidermal water loss, which is an indicator of stratum corneum damage, which may be the primary cause of inflammatory reactions. For this reason, conventional cosmetics have a problem in that harmful side effects occur on the skin, and thus there is a limit in obtaining a skin moisturizing effect and a protective effect.

Accordingly, researches on moisturizers through mechanisms that increase the absorption of water by increasing aquaporins that control epidermal hydration without side effects of conventional moisturizers have been conducted. In humans, three billion water molecules per second pass through the skin through a protein called aquaporin. Aquaporin is divided into several types according to structure and function. Among them, aquaporins-3, which is known to be related to skin, is present in keratinocytes and in the basal layer of the epidermis.

It also increases the water retention of the epidermis, and molecules such as hyaluronic acid play an important role in the skin's hydration process. Hyaluronic acid in the epidermis also plays an important role in skin aging. As age grows, the amount of hyaluronic acid decreases rapidly, resulting in wrinkles. That is, hyaluronic acid is produced in keratinocytes and plays an important role in hydration of the stratum corneum, as well as affects water retention and regulation of water and ion exchange.

On the other hand, by increasing the amount of hyaluronan synthase-2, an enzyme that synthesizes hyaluronic acid, it not only promotes the synthesis of hyaluronic acid, but also affects the ability of the produced hyaluronic acid to bind with water. It also has an effect of increasing the activity of a receptor called CD44, which has been found to be an important problem to identify the components that promote the synthesis of hyaluronic acid.

On the other hand, the pansy extract of the present invention is known to have a skin whitening effect as a melanin production inhibitory ingredient (Japanese Patent Laid-Open No. 2002-003336), but it is not known to increase aquaporin or promote hyaluronic acid synthesis.

The present invention increases the aquaporins-3 related to the maintenance of moisture in the epidermis, increases the synthesis of receptors involved in the binding of hyaluronic acid and hyaluronic acid, thereby inhibiting evaporation of the epidermis and maintaining moisture in the stratum corneum. Various herbal extracts were studied in order to achieve the technical problem showing the effect.

Thus, the pansy extract of the present invention was found to increase the aquaporins-3 (aquaporins-3), and to be effective in moisturizing the skin, such as improved skin moisture retention in relation to hyaluronic acid, and confirmed the present invention to complete the present invention.

In addition, when the pansy extract is provided to the cosmetic composition, the present invention was completed by confirming that the skin moisturizing or skin wrinkle improvement effect is excellent.

The present invention relates to an epidermal moisture control composition containing pansy extract.

In other words, the pansy extract promotes the synthesis of aquaporins-3 related to the maintenance of moisture in the epidermis and increases the synthesis of receptors involved in the binding of hyaluronic acid and hyaluronic acid, thereby inhibiting evaporation of the epidermis and It is characterized by showing the effect of controlling the amount of epidermis by maintaining moisture.

The present invention also relates to a cosmetic composition for improving skin moisturizing or improving skin wrinkles by containing the pansy extract.

Pansy (Viola tricolor L.) is a tritical violet thoracic flower and annual herb, also known as tricolor violet. The height is 10-15cm, the stem grows straight or extends to the side, and many branches are split. The bottom leaf is round oval, and the upper leaf is slightly thin and long spatula. Petioles have long, large chin leaves. Flowers come from leaf axes in March to June, and long peduncles run one by the end. 5 petals. The diameter of the flower is 3-12cm, and there are small race species (小 輪 種) and middle race species (中 輪 種) ㅇ large race species (大 輪 種). White, yellow, purple, and three colors of blood and horticulture varieties, such as monochromatic orange, brownish blue, red, blue. Fruits are capsules and egg-shaped.

The pansy extract is characterized in that the hot solvent is extracted from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, most preferably, by adding ethanol. Check and see if it's the same way-it's the same way)

In addition, pansy extract according to the present invention can be prepared as follows.

Wash the pansy thoroughly with water and dry it in the shade. The dry pansy is placed in the extraction vessel and extracted for a certain period of time at an appropriate temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as a bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of hot water extraction. (Please distinguish between hot water extraction and clear relationship.) Extraction of hot water refers to the same method as hot water extraction, and reflux cooling extraction is a method of obtaining a constant concentration of extract by repeatedly applying a solvent. Ultrasonic extraction is a method in which ultrasonic waves are fired on natural products and extraction solvents to extract the active ingredients of natural products by using the vibration of molecules to cause contraction and expansion of ultrasonic waves in natural products and extraction solvents. This is how you can reduce it. -The hot water extraction method is the most commonly used method, so this is the main topic.

The deposition temperature is 30 ~ 90 ℃, more preferably 60 ~ 80 ℃, most preferably maintained at about 60 ℃ is most effective in the prevention of decay, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After the pansy extract is obtained, solids may be removed using a filter paper, the suspension may be centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

The pansy extract in the composition of the present invention may be contained from 0.005 to 50% by weight relative to the total weight of the composition.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the pansy extract.

Cosmetics prepared with a cosmetic composition containing the pansy extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the pansy extract of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be applied topically or systemically applied commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to pansy extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Pansy Extract

50 g of pansy was finely crushed in a crusher and immersed in 200 ml of 50% ethanol and warmed in an 80 ° C. water bath for 2 hours or more. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

(Test Example 1) Promotion of aquaporins-3 synthesis

The effect of increasing the synthesis of aquaporins-3, which pansy extract plays an important role in water migration, was confirmed by Western blotting after extract treatment to cells.

24 hours after inoculation of keratinocytes, 0.25%, 0.5% and 30ng / ml of EGF (epidermal growth factor) of Pansy extract of Preparation Example 1 were added and cultured in KSFM medium, 37 ° C., and 5% CO 2. After incubation for 24 hours, the cells are recovered and crushed to extract only proteins and stored at -80 ° C. The extracted protein is quantified by BCA assay (Sigma Cat. No. BCA1). Dilute to 30 μg of protein in each sample (make sure the units are correct). After dilution, electrophores in 12% SDS-gel and transfer to P membrane (Millipore IPVH, Cat. No. 15150). After reacting with the primary antibody (rabbit anti-aquaporins-3 antibody), the remaining antibody is washed and removed and reacted with the secondary antibody (rabbit HRP-coupled anti-IgG antibody). After removing the remaining antibody, a reagent (peroxidase substrate and chromogen solution) which reacts with the antibody to generate a color reaction is added. The color bands were quantified by image analysis (BIO1D, Vilber-Lourmat, France).

Level of aquaporins-3 (%) Control 100 EFG (30 ng / ml) 146 * 0.25% Pansy Extract 120 0.5% Pansy Extract 144 *

*: Significant results according to student's test (P <0.05)

      As a result of the test of Table 1, it was found that 44% increase in the synthesis of aquaporins-3 (aquaporins-3) when the pansy extract 0.5% treatment on keratinocytes. Therefore, the pansy extract was found to increase the water supply from the dermis to the stratum corneum by smoothing the movement of water.

Test Example 2 Synthesis of Hyaluronic Acid

Hyaluronic acid is a viscous substance like cement that fills cells and plays an important role in skin elasticity and epidermal moisture retention. The increase in the synthesis of hyaluronic acid during pansy extract treatment was confirmed through the hyaluronic acid measurement test after applying the extract to the cells.

Keratinocytes were seeded and incubated in KSFM medium, 37 ° C., 5% CO 2 for 24 hours. Pansy extract of Preparation Example 1, 0.5%, 1% was added to the medium and incubated for 72 hours, after which the supernatant was taken and stored at -80 ° C. The amount of hyaluronic acid is measured using an ELISA assay kit (Biogenic Cat. No. O29001).

Hyaluronic acid ( ㎍ / ml) (Please check if the unit is correct.) Hyaluronic acid (%) Control 3.19 100 0.5% Pansy Extract 3.31 104 1% Pansy Extract 3.94 123 *

*: Results significant according to studant's test (P <0.01)

      As a result of the test of Table 2, it was confirmed that the amount of hyaluronic acid present in the culture supernatant increased by 23% when 1% of the pansy extract was treated to keratinocytes.

Test Example 3 Promotion of Hyaluronan Synthase-2 Synthesis

       Hyaluronan synthase-2 (hyaluronan synthase-2) is an enzyme involved in the synthesis of hyaluronic acid, the pansy extract induces the expression of the mRNA encoding hyaluronan synthase-2 (hyaluronan synthase-2). The pansy extract was treated to keratinocytes and confirmed the increase of mRNA expression through RT-PCR.

        1% of Pansy extract of Preparation Example 1 was added to keratinocytes and cultured for 24 hours. After culture, RNA was extracted and PCR was performed using reverse transcriptase and cDNA (complementary DNA). Beta-actin was used as standard. It was quantified by electrophoresis of agarose gel and quantified by image analysis (BIO-PROFIL system).

mRNA (%) Control 100 1% Pansy Extract 138 *

*: Significant results according to student's test (P <0.05)

     As a result of the test in Table 3, when 1% of pansy extract was treated on keratinocytes, mRNA expression of hyaluronan synthase-2 was increased by 38%.

Test Example 4 Synthesis of CD44

       CD44 is a receptor of hyaluronic acid, and pansy extract has an effect of promoting the synthesis of CD44. After treatment to keratinocytes was measured by flow cytometry.

       The keratinocytes were treated with Pansy extract 0.5, 1, 2% of Preparation Example 1 and Eng 30ng / ml and then incubated for 72 hours. The medium was removed, reacted with CD44 antibody (R & D systems-UK) at 45 ° C. for 45 minutes and again with secondary antibody Alexa Fluor-488 (Interchim, France, Cat. No. A11017). The immunolabeled cells were analyzed by FACS Vantage cell sorter (BECTON DICKSON, USA).

CD44 (%) Control 100 EFG (30 ng / ml) 126 * 0.5% Pansy Extract 100 1% Pansy Extract 120 2% Pansy Extract 129 **

*: Significant result according to student's test (P <0.10)

**: Significant result according to student's test (P <0.05)

      As a result of the test in Table 4, when 2% of pansy extract was treated on keratinocytes, the synthesis of CD44 was increased by 29%. It was also found that the concentration increased depending on the extract.

Preparation Example 2 Examples and Comparative Examples

To prepare a cosmetic composition containing the pansy extract of Preparation Example 1, in order to compare the cosmetic composition lacking the pansy extract was prepared in the composition ratio of Table 5.

division Content (% by weight) ingredient Example Comparative example Pansy extract 4.0 - Cetearyl Octanoate 5.0 5.0 Isopropyl Palmitate 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 Polysorbate 60 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 Spices, preservatives dose dose Purified water Remaining amount Remaining amount Sum 100 100

(Test Example 4) Clinical effect of improving the moisturizing power

The clinical effect of improving skin moisturizing power of the cosmetic containing pansy extract of the present invention was measured. Twenty females aged 36 to 59 years (mean age 48) were selected and those with dry skin whose skin moisture level was less than 65 were selected. After determining the two sites to be measured and measuring the moisture content of the skin three times each, and formulated according to Table 5 above and applying for 4 weeks (two times / day), a corneometer (Corneometer Courage) which is a skin moisture content measuring device & Khazaka, Germany) to measure the moisture in the epidermal layer just below the stratum corneum.

Comparative example Example Subject D0 D28 D0 D28 One 65 41 65 37 2 63 42 54 42 3 51 63 54 49 4 49 - 52 - 5 60 45 65 69 6 65 45 55 44 7 36 37 51 50 8 55 29 61 45 9 63 68 54 59 10 26 28 39 58 11 33 28 29 34 12 30 28 45 40 13 41 37 61 45 14 46 41 32 30 15 41 26 44 40 16 60 62 45 51 17 64 47 64 51 18 44 44 25 29 19 61 56 46 53 20 40 55 48 67 Average 54 45 54 47 △ 28 13%

      From the results shown in Table 6, when looking through the relative numerical calculation before and after the application of the example, it was confirmed that the water content increased by 13% after 28 days of application of the example containing pansy extract as a result of measuring the skin moisture there was. This effect was observed in 68% of the subjects.

      As a result, it can be seen from the results shown in Table 6 that the cosmetics containing the herbal extracts prepared by the extraction example according to the present invention is to improve the skin moisture, it shows that the excellent effect on the skin moisturizing power Can be.

Experimental Example 4 Clinical Effect of External Wrinkle Improvement

The clinical effect of skin wrinkle improvement on the cosmetic containing pansy extract of the present invention was measured. Twenty women aged 42 to 55 years (mean age 50) were included. After formulating according to Table 5 and applying for 28 days (2 times / day), a replica was prepared and analyzed using S.I.A (silicone image analysis).

Can be wrinkled Comparative example Experimental Example Subject D0 D28 D0 D28 One 197 216 392 257 2 212 324 297 329 3 185 162 261 288 4 344 307 216 277 5 155 190 190 242 6 162 153 195 222 7 167 142 152 150 8 150 215 144 151 9 154 165 197 166 10 73 173 136 135 11 174 135 - 123 12 150 224 292 229 13 69 108 85 84 14 194 - 136 - 15 153 185 124 187 16 116 162 176 137 17 205 199 152 117 18 147 195 218 138 19 211 179 203 200 20 233 210 183 161 21 138 154 135 170 22 123 127 131 - 23 296 222 185 163 24 226 282 237 239 Average 178 198 199 192 △ (%) -6

All wrinkles Comparative example Experimental Example Subject D0 D28 D0 D28 One 15.36 11.89 20.98 12.50 2 27.36 20.86 30.87 25.27 3 16.72 20.84 15.45 23.44 4 23.44 18.33 27.22 19.93 5 19.69 26.97 27.69 24.99 6 17.93 14.13 27.09 16.66 7 17.41 20.27 31.57 25.53 8 19.56 28.00 19.24 16.11 9 24.75 24.56 27.63 17.24 10 4.38 13.79 18.75 31.88 11 21.62 11.66 - 24.09 12 14.32 28.94 20.97 14.01 13 14.72 15.20 15.47 8.33 14 20.35 - 19.39 - 15 25.32 27.60 20.41 22.58 16 23.98 19.51 23.98 22.74 17 20.40 17.82 10.86 7.26 18 34.70 33.80 24.57 17.32 19 28.21 27.26 26.41 24.29 20 39.83 28.26 26.29 24.79 21 23.28 31.59 31.73 28.98 22 10.91 8.70 10.40 - 23 13.15 8.13 20.08 18.25 24 10.57 17.66 29.98 22.50 Average 20.72 21.69 23.68 20.22 △ (%) -13

Wrinkle Length Comparative example Experimental Example Subject D0 D28 D0 D28 One 86.72 114.61 165.28 95.59 2 153.38 171.49 189.27 168.76 3 90.74 101.49 112.36 128.78 4 170.43 156.43 117.19 121.60 5 84.54 127.01 162.87 159.85 6 98.70 71.82 145.99 119.16 7 102.42 88.05 140.58 128.12 8 81.14 135.01 102.03 98.12 9 112.58 126.07 136.10 101.95 10 31.87 87.17 77.69 138.12 11 107.09 52.40 34.87 50.39 12 92.19 158.44 158.01 104.69 13 43.57 65.44 48.47 35.17 14 118.77 126.14 92.00 115.39 15 135.07 149.56 95.50 111.29 16 101.91 98.91 113.08 96.05 17 111.33 110.74 89.27 53.93 18 125.77 160.78 134.65 88392 19 145.61 140.48 120.11 125.20 20 174.48 133.66 125.86 108.57 21 102.50 99.56 109.78 122.73 22 58.99 59.05 62.86 90.69 23 105.59 71.95 86.64 81.56 24 99.61 115.00 170.45 120.29 Average 107.15 118.29 123.87 109.93 △ (%) -13

As shown in Table 7, 8, and 9 was confirmed that the effect on the skin wrinkles after 28 days of application of the Example. The total wrinkle area and wrinkle length were reduced by 13%, the number of wrinkles was also reduced by 6%, and the wrinkle area was reduced in 66% of the total subjects.

As a result, the fact that can be confirmed through the results shown in Tables 7, 8 and 9 is a cosmetic containing the herbal extract prepared by the extraction example according to the present invention to enhance the skin wrinkle improvement effect, excellent skin anti-aging It can be seen that it is effective.

       Based on the results of the previous experimental example, to present a cosmetic composition containing the pansy extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

     Formulation example of the flexible lotion containing pansy extract was prepared as shown in Table 10.

Raw material Content (% by weight) Pansy extract 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

Formulation Example 2 Nutrition Cream

Formulation example of nutrition cream containing pansy extract was prepared as shown in Table 11.

Raw material Content (% by weight) Pansy extract 2.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

 As described above, the present invention, the pansy extract promotes the synthesis of aquaporines-3 (related to water retention of the epidermis) and increases the synthesis of the receptors involved in the binding of hyaluronic acid and hyaluronic acid to increase the moisture of the epidermis It suppresses evaporation and maintains moisture in the stratum corneum. When the composition containing the pansy extract is used in the cosmetic composition, it is useful for the cosmetic composition for moisturizing or wrinkle improvement.

Claims (9)

Epidermal moisture control composition containing pansy extract. The method of claim 1, wherein the composition is epidermal moisture control composition characterized in that the maintenance of epidermal moisture through promoting the synthesis of aquaporines-3 (aquaporines-3). The epidermal moisture control composition of claim 1, wherein the composition increases the synthesis of hyaluronic acid and a receptor involved in the binding of hyaluronic acid. The epidermal moisture control composition according to claim 4, wherein the composition inhibits the evaporation of the epidermis and maintains the moisture of the stratum corneum. The composition of claim 1, wherein the pansy extract is hydrothermally extracted. 6. The epidermal moisture control composition according to claim 5, wherein the hot water extraction is performed by adding a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. Cosmetic composition for skin moisturizing enhancement or skin wrinkles containing pansy extract. The cosmetic composition according to claim 7, wherein the content of the pansy extract is 0.005 to 50% by weight based on the total weight of the cosmetic composition. According to claim 7, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack Cosmetic composition, characterized in that the selection of any one selected from the group consisting of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.