KR102202210B1 - Moisturizing cosmetic composition comprising nutmeg extract, hyaluronic acid and sericin - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing moisture containing nutmeg extract, hyaluronic acid and sericin, wherein the cosmetic composition for moisturizing moisture containing nutmeg extract, hyaluronic acid and sericin of the present invention not only moisturizes skin, but also differentiates skin keratinocytes. It can be useful as a skin moisturizing cosmetic as it can promote and restore skin barrier function to relieve the skin's pulling and surface roughness.

Description

Moisturizing cosmetic composition comprising nutmeg extract, hyaluronic acid and sericin}

The present invention relates to a cosmetic composition for moisturizing moisture containing nutmeg extract, hyaluronic acid and sericin.

In the human body, the skin is an organ that functions as a protective film that protects the living body from the external environment. It prevents the loss of biological components in the human body, such as water or electrolytes, and at the same time prevents harmful substances from entering the human body. . Such skin is largely divided into an epidermal layer, a dermal layer, and a subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes, and since the stratum corneum layer at the outermost part of the skin directly contacts the external environment, strong resistance to physical, chemical, or material penetration is required, preventing the loss of moisture from the human body to the outside. At the same time as zooming, it must maintain its flexibility by retaining adequate moisture on its own.

In general, the amount of moisture in the skin is about 70% in the dermis layer, but decreases toward the epidermis layer to become about 10 to 30% in the stratum corneum layer. The water supplied from the dermal layer moves to the upper part of the stratum corneum mainly by passive diffusion and is finally discharged to the outside. This is called Trans Epidermal Water Loss (TEWL), and it is known that sebum components and epidermal lipids, which are the lipid membrane components of the stratum corneum, are responsible for maintaining such transdermal water loss at an appropriate level. In addition, the stratum corneum is known to play an important role in moisturizing the skin because there is a substance with hydrophilic moisture retention called Natural Moisturizing Factor (NMF). When the moisture in the normal stratum corneum is maintained at about 10 to 30%, the skin is smooth and soft, and the body's protective function is normally exerted. However, when the moisture content of the stratum corneum layer is less than 10%, the skin becomes rough and the body's protective function is lost, leading to aging. For example, in the case of dry skin, a scaling phenomenon occurs in which the keratinocytes are weakened and keratinocytes are loosely peeled off from the skin surface like scales. This is because it is less than normal skin. In addition, even with healthy skin, the skin condition may deteriorate due to a lack of moisture due to harsh external environments such as wind, cold weather, sunlight, cleansing, and shaving. Therefore, it is very important to properly maintain the moisture content of the stratum corneum layer of the skin.

Recently, it has been argued that the differentiation of the stratum corneum is important in preventing the drying of skin moisture, and the differentiation process that develops from keratinocytes in the base layer to keratinocytes in the outermost stratum corneum must be performed normally to play a role as a barrier for retaining skin moisture. It turns out that there is. In other words, during the process of keratinization, the cells generate natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes firm and flexible and can function as a barrier. Although it was not known about the differentiation process of the stratum corneum of the skin, two types of proteases that are activated in the last stage of the recent keratinization process point to desmosomal protein, an important component of connective tissue. It was found that it decomposes, and in this process, it was also found that the moisture content of the stratum corneum had a great influence.

Therefore, as people's age increases, the tendency of the skin to become dry is observed.As a result of this, physiologically, the dropout time of the stratum corneum increases, the ability of epidermal cells to synthesize lipids decreases, and the amount of moisturizing factors and lipids in the stratum corneum of the skin decreases. It becomes. This is because the normal differentiation process did not occur in the epidermal cells due to the decrease in the ability to form the skin barrier function and the excessive keratin formation as the age increases. Therefore, by promoting the differentiation of keratinocytes, it induces strengthening of the skin barrier, thus enhancing the skin moisture retention function and the protective function from the external environment. Accordingly, the role of skin moisturizers is also changing from simply increasing the moisture of the structural components of the stratum corneum to being involved in the stratum corneum differentiation process.

Accordingly, the present inventors confirmed that the cosmetic composition containing hyaluronic acid, sericin, and nutmeg extract is actually excellent in skin moisturizing effect when applied to human skin and completed the present invention.

Korean Patent Registration No. 10-1082644

An object of the present invention is to provide a cosmetic composition for moisturizing skin.

Another object of the present invention is to provide a cosmetic composition for strengthening the skin barrier.

To achieve the above object,

The present invention nutmeg ( Myristica fragrans ) extract;

Hyaluronic acid; And

Sericin; It provides a skin moisturizing cosmetic composition comprising a.

In addition, the present invention nutmeg ( Myristica fragrans ) extract;

Hyaluronic acid; And

Sericin; It provides a cosmetic composition for strengthening the skin barrier comprising a.

The cosmetic composition for moisturizing moisture containing nutmeg extract, hyaluronic acid and sericin of the present invention not only moisturizes the skin, but also promotes the differentiation of keratinocytes and restores the skin barrier function, thereby relieving the skin tension and roughness of the surface, thus moisturizing the skin. It can be useful as a cosmetic product.

1 shows the moisture retention ability of the cosmetic composition of the present invention.
Figure 2 shows the cytotoxicity of the cosmetic composition of the present invention.

Hereinafter, the present invention will be described in detail.

Cosmetic composition for moisturizing skin or strengthening skin barrier

The present invention nutmeg ( Myristica fragrans ) extract; Hyaluronic acid; And sericin; It provides a cosmetic composition for skin moisturizing or strengthening the skin barrier comprising a.

The extract according to the present invention can be used to separate and obtain an extract using a method known in the art for extraction and separation, and the extract defined in the present invention is an active ingredient prepared by the above method using an appropriate solvent. The raw material can be extracted as it is or by grinding. At this time, any suitable solvent that can be used to obtain the extract may be used as long as it is an acceptable solvent in the art, and water or an organic solvent may be used. For example, alcohol having 1 to 4 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether , Benzene, chloroform, ethyl acetate, methylene chloride, hexane, and various solvents such as cyclohexane may be used alone or in combination. It is not limited.

Preferably, the extract may be extracted using at least one extraction solvent selected from the group consisting of water, an anhydrous or water-containing lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent, or a mixed solvent thereof, More preferably, it may be extracted using one or more extraction solvents selected from water or ethanol.

As the extraction method, any one of methods such as hot water extraction, cold precipitation extraction, reflux cooling extraction, solvent extraction, steam distillation method, ultrasonic extraction method, elution method, and compression method may be used. In addition, the desired extract may be further subjected to a conventional fractionation process, or may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

For example, the extract contained in the composition of the present invention may be used as it is while the primary extract extracted by the above-described solvent extraction method is centrifuged, filtered, concentrated, freeze-dried, and stored in a refrigerator. It can be prepared in powder form by additional processes such as distillation and freeze drying or spray drying. Further, the primary extract was further purified by using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get

Therefore, in the present invention, the extract is a concept including all extracts, fractions and purified products obtained in each step of extraction, fractionation, or purification, their dilutions, concentrates, or dried products.

In one embodiment of the present invention, the cosmetic composition for moisturizing the skin may further include an extract of Okyongsan ( Gleditschia australis thorn ).

In one embodiment of the present invention, hyaluronic acid 10 to 30 parts by weight based on 1 part by weight of the nutmeg extract, characterized in that it comprises 0.5 to 3.5 parts by weight of sericin, preferably hyaluronic acid based on 1 part by weight of the nutmeg extract It may be used, including 15 to 25 parts by weight of Ronic acid and 1 to 3 parts by weight of sericin.

In one embodiment of the present invention, the cosmetic composition for moisturizing the skin may not only moisturize the skin, but also promote the differentiation of skin keratinocytes and restore the skin barrier function to relieve the pulling of the skin and roughness of the surface.

In one embodiment of the present invention, the cosmetic composition for strengthening the skin barrier may prevent skin dryness or skin inflammation due to damage to the skin barrier by strengthening the skin barrier function.

The cosmetic composition according to the present invention is from the group consisting of lotion, nutritional lotion, nutritional cream, massage cream, essence, paste, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax and spray. It may be one or more selected formulations, and may be used in the form of a mask pack. Other ingredients other than the above essential ingredients may be appropriately selected and blended by a person skilled in the art without difficulty according to other formulations or purposes of use.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and/or bis. It may be provided in the form of a warm vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of an adjuvant that can be applied topically or systemically used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventional in the field. It can be manufactured according to the method.

In addition, the cosmetic composition of the present invention nutmeg ( Myristica fragrans ) extract; Hyaluronic acid; And sericin; In addition to fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers , Sequestering agents and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants used as. These adjuvants are introduced in amounts commonly used in the field of cosmetology.

In one embodiment of the present invention, in addition to the above ingredients, the cosmetic composition includes glycerin, silk amino acid solution, vitamin E, okyong acid extract, niacinamide, olive liquid, daisy oil, tea tree oil, rosewood oil, lemon oil, camellia It may further include one or more components selected from the group consisting of oil, 1,2-hexanediol, and neroli water. At this time, based on 1 part by weight of the nutmeg extract, glycerin 7.0-9.0 parts by weight, silk amino acid solution 14.0-16.0 parts by weight, vitamin E 2.0-4.0 parts by weight, Okyong acid extract 0.1-1.5 parts by weight, niacinamide 0.1-2.0 parts by weight , Olive liquid 2.0-4.0 parts by weight, Daisy oil 0.01-0.3 parts by weight, Tea tree oil 0.01-0.3 parts by weight, Rosewood oil 0.01-0.3 parts by weight, Lemon oil 0.01-0.3 parts by weight, Camellia oil 4.0-6.0 parts by weight , 1,2-hexanediol 1.0-3.0 parts by weight, neroli water 35 to 55 parts by weight may be included, but is not limited thereto.

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

<Preparation Example 1> Nutmeg extract preparation

To 20 g of dry pulverized nutmeg ( Myristica fragrans ), 200 ml of 70% ethanol was added as an extraction solvent, and extracted in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 80° C. for 8 to 14 hours to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh) to obtain a nutmeg filtrate. After removing the solvent from the nutmeg filtrate using a rotary evaporator (DL10-3000, Greatwall, 20L), a nutmeg extract was prepared. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 4°C.

<Preparation Example 2> Preparation of Okyongsan extract

To 100 g of dry pulverized Okyong acid ( Gledischia Australis Thorn ), 20 times of distilled water was added as an extraction solvent, and extracted in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 80° C. for 8 to 14 hours to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh) to obtain a filtrate of Okyongsan. The Okyongsan filtrate was removed from the solvent using a rotary evaporator (DL10-3000, Greatwall, 20L) to prepare an Okyongsan extract. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 4°C.

<Examples 1 to 2 and Comparative Examples 1 to 4> Preparation of serum containing hyaluronic acid, sericin, and nutmeg extract

Hyaluronic acid was purchased and used from Lohas Nature Co., Ltd., and sericin was purchased and used from pincredit bio-tech co.,ltd (Xi'an city, China).

A serum containing hyaluronic acid, sericin, and nutmeg extract (Preparation Example 1) was prepared in the composition of Table 1 and Table 2 below. At this time, in Tables 1 and 2 below, the content of the remaining components is expressed in parts by weight based on 100 parts by weight of the total composition.

Parts by weight Hyaluronic acid Sericin Nutmeg extract Okyongsan extract Example 1 10 One 0.5 - Example 2 10 One 0.5 4 Comparative Example 1 10 - - - Comparative Example 2 - One - - Comparative Example 3 - - 0.5 - Comparative Example 4 10 One - -

Other ingredients other than the active ingredients in Table 1 are as shown in Table 2 below.

Other ingredients Parts by weight Glycerin 8 Silk Amino Acids 15 Vitamin E (Tocopherol) 3 Niacinamide One Olive Liquid (INCI: Sodium PEG-7 Olive Oil Carboxylate) 3 Daisy essential oil 0.1 Rosewood essential oil 0.07 Tea tree essential oil 0.07 Lemon essential oil 0.07 Camellia essential oil 5 1,2-hexanediol (1,2-Hexanediol) 2 Neroli water Balance

<Experimental Example 1> Evaluation of water retention ability

The water retention capacity of Examples 1 to 2 and Comparative Examples 1 to 4 was measured. Each of the above Examples 1 to 2 and Comparative Examples 1 to 4 was added to a Petri dish, and paper of an appropriate size was wetted, and the paper was weighed. Paper containing Examples 1 to 2 and Comparative Examples 1 to 4, respectively, was placed in an empty Petri dish and the weight of the paper was measured over time to confirm the moisture retention ability. Purified water was used as a negative control.

The water retention ability was evaluated using the following formula.

Moisture retention capacity (%) = (weight after elapsed time-paper weight) × 100/ (initial weight-paper weight)

Moisture retention capacity (%) 0 minutes 30 minutes 60 minutes 90 minutes 120 minutes 150 minutes 180 minutes Example 1 100 100 93.7 81.2 74.4 62.1 54.9 Example 2 100 100 94.1 83.7 76.9 67.8 57.1 Comparative Example 1 100 55.7 41.85 38.76 26.31 21.05 18.06 Comparative Example 2 100 51.8 44.02 31.21 21.08 19.44 16.20 Comparative Example 3 100 57.1 49.85 35.26 22.75 20.59 17.66 Comparative Example 4 100 71.8 64.2 55.3 42.5 31.7 26.7 Con (purified water) 100 0 0 0 0 0 0

As shown in Table 3 and FIG. 1, Example 1 (hyaluronic acid, sericin, and nutmeg extract) and Example 2 (hyaluronic acid, sericin, nutmeg extract, and Okyongsan extract) of the present invention were comparative examples for the same time. It can be seen that the water retention ability is remarkably superior to those of 1 to 4.

<Experimental Example 2> Measurement of keratinocyte differentiation promoting effect

In order to examine the differentiation promoting effect of keratinocytes of Examples 1 to 2 and Comparative Examples 1 to 4, the amount of CE (Cornified Envelop) generated during differentiation of keratinocytes was measured using absorbance.

Human keratinocytes were placed in a culture flask and attached to the bottom, and then Examples 1 to 2 and Comparative Examples 1 to 4 were treated at a concentration of 10 ppm in the culture medium until the cells grew about 70 to 80% of the floor area. Incubated for 5 days. Then, the cultured cells were harvested and washed with PBS (Phophate buffered saline), and then a 10 mM Tris-HCl buffer solution containing 2% sodium dodecyl sulfate (SDS) and 20 mM dithiothreitol (DTT). pH74) 1 ml was added, sonication, boiling, and centrifugation were performed, and the precipitate was suspended again in 1 ml of PBS, and the absorbance at 340 nm was measured. Separately, a part of the sonicated solution was taken to measure the protein content, and used as a standard for evaluating the degree of cell differentiation. The results are shown in Table 4 below.

Differentiation ability in keratinocytes (%) Example 1 235 Example 2 246.75 Comparative Example 1 105 Comparative Example 2 108 Comparative Example 3 106 Comparative Example 4 141

As shown in Table 4, in the case of Example 1 (hyaluronic acid, sericin, and nutmeg extract) and Example 2 (hyaluronic acid, sericin, nutmeg extract, and Okyongsan extract) compared to Comparative Examples 1 to 4, differentiation of keratinocytes It was confirmed that the promoting effect was remarkable.

<Experimental Example 3> Measurement of skin barrier function recovery effect

In order to measure the effect on the recovery of the skin barrier function damaged by skin damage, the upper arm of 30 adult men and women was applied with 20 µl of SLS 10% aqueous solution to a fin-chamber and applied for 16 hours to damage the skin barrier. While applying 1 to 2 and Comparative Examples 1 to 4, the amount of transdermal water loss (TEWL) was measured once a day for 7 days, and the results are shown in Table 5 below.

Transdermal moisture loss (TEWL) change (%) Before treatment 1 day 2 days 3 days 4 days 5 days Example 1 100 91 74 52 30 17 Example 2 100 96 78 55 32 18 Comparative Example 1 100 103 94 81 64 52 Comparative Example 2 100 99 92 88 62 47 Comparative Example 3 100 98 90 80 60 45 Comparative Example 4 100 97 89 77 54 40

As shown in Table 5, when treating Comparative Examples 1 to 4 compared to Example 1 (hyaluronic acid, sericin, and nutmeg extract) and Example 2 (hyaluronic acid, sericin, nutmeg extract, and Okyong acid extract), It can be seen that the transdermal water loss returns to normal and the barrier damage is restored.

<Experimental Example 4> Confirmation of skin moisturizing power improvement effect

In order to find out the effect of improving the skin moisturizing power of Examples 1 to 2 and Comparative Example 4, an experiment was conducted as follows. After selecting 30 people in their 20s to 40s who do not have skin diseases, dividing the entire thin area into a size of 3 x 3 cm by using an oil pen and applying Example 1 to one side and Comparative Example 4 to the other twice a day for 4 weeks. I made it. At this time, before starting the application, measure the skin conductivity using a Corneometer (CM820 Corage Khazaka Electronic GmbH, Germany) under constant temperature and humidity conditions (24 degrees, humidity 40%) and take the basic value, 1 week, 2 weeks, 4 The skin conductivity after the lapse of weeks was measured to evaluate the rate of increase. The results are shown in Table 6 below.

Skin conductivity increase rate (%) Before application 1 week later 2 weeks later 4 weeks later Example 1 100 57 50 63 Example 2 100 60 53 67 Comparative Example 4 100 21 19.5 27

As shown in Table 6, in the case of Examples 1 and 2, which are cosmetics containing nutmeg extract, hyaluronic acid and sericin of the present invention, it was confirmed that the increase rate of skin conductivity was very excellent compared to Comparative Example 4 (including hyaluronic acid and sericin). Since the skin conductivity is proportional to the amount of skin moisture, it can be seen that the cosmetic composition including nutmeg extract, hyaluronic acid and sericin of the present invention has excellent skin moisturizing effect.

<Experimental Example 5> Cytotoxicity test

In order to find out that it can be used as a safe cosmetic material, fibroblasts were used to confirm cytotoxicity. Fibroblasts were cultured in a medium containing DMEM (Dulbecco'Modified Eagle'), 10% FBS (Fetal Bovine Serum), and 1% Penicillin/Streptomycin, and in a 37 ^o C incubator continuously supplying 5% CO ₂ Was maintained. The cells were cultured on a 96-well plate, and when grown over 90% (90% of Visual Confluency), the final concentrations of Examples 1 and 2 were 0.01, 0.05, 0.1, 0.25, 0.5, 1 and 2.5%, respectively. After the addition so as to be treated for 48 hours. After removing the culture medium, the cells on the 96-well plate were washed 3 times with PBS (Phosphate-buffered saline). Next, after adding 10 μL of CCK-8 solution (CK04, Dojindo Molecular Technologies, USA), the reaction was performed at 37 ^° C for 4 hours, and then the absorbance at OD=450nm was measured to confirm the survival rate of the cells.

As a result, as shown in Fig. 2, the cell viability was found to be 85% or more at all concentrations tested.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (6)

Nutmeg ( Myristica fragrans ) extract;
Hyaluronic acid; And
Sericin; Skin moisturizing cosmetic composition comprising a.
Nutmeg ( Myristica fragrans ) extract;
Hyaluronic acid; And
Sericin; Cosmetic composition for strengthening the skin barrier comprising a.
The method according to claim 1 or 2,
The nutmeg extract is a cosmetic composition, characterized in that it is extracted using one or more extraction solvents selected from the group consisting of water, anhydrous or aqueous lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, hydrocarbon solvents, or mixed solvents thereof. .
The method according to claim 1 or 2,
The composition is a cosmetic composition, characterized in that it may further include an extract of Okyongsan ( Gleditschia australis thorn ).
The method according to claim 1 or 2,
Cosmetic composition comprising 10 to 30 parts by weight of hyaluronic acid and 0.5 to 3.5 parts by weight of sericin based on 1 part by weight of the nutmeg extract.
The method according to claim 1 or 2,
The cosmetic composition includes lotion, nutritional lotion, nutritional cream, moisture cream, serum, cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax and spray Cosmetic composition, characterized in that at least one formulation selected from the group consisting of.

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