KR102148095B1 - Cosmetic composition for anti-aging or anti-wrinkle comprising epidermal growth factor, sericin, Foeniculum vulgare extract and Eugenia caryophyllus extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging or improving wrinkles, including epidermal growth factor (EGF), sericin, fennel ( Foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) extract, the present invention According to the cosmetic composition according to the antioxidant effect, as well as promoting collagen production, inhibiting elastase activity, and can improve the wrinkles and elasticity of the skin can be useful as a cosmetic for skin aging prevention and wrinkle improvement.

Description

Cosmetic composition for anti-aging or anti-wrinkle comprising epidermal growth factor, sericin, Foeniculum vulgare extract and Eugenia caryophyllus extract}

The present invention relates to a cosmetic composition for preventing skin aging or improving wrinkles, including epidermal growth factor (EGF), sericin, fennel ( Foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) extract.

The skin is the largest organ in the body and plays a very important role in protecting the body from physical abrasions, preventing the loss of water in the body, protecting it from harmful ultraviolet rays from the sun, and regulating the body's body temperature.

Human skin undergoes various physical and chemical changes during the aging process. The aging of the skin can be caused by factors such as ultraviolet rays, stress, disease state, environmental factors, wounds, etc., and free radicals are activated as we age, and if this condition is deepened, it destroys the antioxidant defenses existing in the living body. It damages cells and tissues and promotes adult diseases and aging. More specifically, skin cells and tissues are destroyed by oxidation of lipids, proteins, polysaccharides, nucleic acids, etc., which are major components of the skin, resulting in skin aging.

Elastase is an enzyme that degrades elastin, an important substrate for maintaining skin elasticity in the dermis, and is a non-specific hydrolytic enzyme capable of decomposing collagen, another important substrate protein. Therefore, elastase inhibitors have an effect of improving skin wrinkles, and ursolic acid and the like are used as elastase inhibitors. Elastase is known as an enzyme that is the main cause of wrinkles by breaking down elastin, an insoluble elastic fiber protein of animal connective tissue, and breaking the network structure of the dermal tissue of the skin. In the dermal tissue of the skin, collagen and elastin, which are related to the elasticity of the skin, form a network structure. When this network structure is broken, that is, elastin is decomposed by elastase, resulting in sagging and wrinkles of the skin, resulting in endogenous skin aging. do. Therefore, skin aging can be suppressed by lowering the activity of elastase, an elastin-degrading enzyme, which is one of the main causes of skin aging, and studies on natural products having elastin-degrading enzyme inhibitory activity are being continued.

Accordingly, the inventors of the present invention were studying the development of cosmetics using natural plant ingredients capable of exerting anti-aging effects, and a cosmetic composition including epidermal growth factor, sericin, fennel extract and clove extract was used for antioxidant and skin wrinkles. The present invention was completed by confirming that the improvement effect was excellent.

Korean Patent Publication No. 10-2019-0001628

An object of the present invention is to provide a cosmetic composition for preventing skin aging caused by oxidative stress.

Another object of the present invention is to provide a cosmetic composition for preventing or improving skin wrinkles.

To achieve the above object,

The present invention epidermal growth factor (epidermal growth factor, EGF); And

Sericin, foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) provides a cosmetic composition for preventing skin aging due to oxidative stress comprising at least one component selected from the group consisting of extract.

In addition, the present invention epidermal growth factor (epidermal growth factor, EGF); And

Sericin, foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) provides a cosmetic composition for preventing or improving skin wrinkles comprising at least one component selected from the group consisting of extracts.

The cosmetic composition comprising the epidermal growth factor, sericin, fennel extract and clove extract according to the present invention promotes collagen production, inhibits elastase activity, as well as antioxidant effect, and can improve skin wrinkles and elasticity. It can be useful as a cosmetic for anti-aging and wrinkle improvement.

Hereinafter, the present invention will be described in detail.

For preventing or improving skin aging or skin wrinkles Cosmetic Composition

The present invention epidermal growth factor (epidermal growth factor, EGF); And

Sericin, foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) provides a cosmetic composition for preventing skin aging due to oxidative stress comprising at least one component selected from the group consisting of extract.

In addition, the present invention epidermal growth factor (epidermal growth factor, EGF); And

Sericin, foeniculum vulgare ) extract and clove ( Eugenia caryophyllus ) provides a cosmetic composition for preventing or improving skin wrinkles comprising at least one component selected from the group consisting of extracts.

In the cosmetic composition according to the present invention, the epidermal growth factor is one of the growth factors that regulate the proliferation, differentiation and survival of various cells by binding to the epidermal growth factor receptor (EGFR). In particular, it is known to play a major role in re-epithelialization by promoting the proliferation and cell migration of keratinocytes, thereby having an excellent function in wound healing. In the present invention, nano EGF (Epidermal Growth Factor/1ppm) known as Human Oligopeptide-1 was purchased and used.

In the cosmetic composition according to the present invention, the sericin is also referred to as silk glue and silk glue, and the cocoon fiber is covered with two layers of fibroin and three layers of sericin. In 1865, E. Kramer separated from cocoon fibers and named it sericin after the Latin word sercum and Greek serikon, meaning silk. The rough and hard feeling of raw silk is because sericin is attached to it, and when refined, sericin melts and disappears, giving it the unique texture of silk. Sericin has a distinctly high content of about 37 mol% of serine in the amino acid composition, and it dissolves in hot water and gels when cooled.

In the cosmetic composition according to the present invention, the fennel is a perennial plant belonging to the family Unbelliferae, called fennel in the West, and uses leaves, stems, and flowers to remove odor or improve digestion. Also called'herbs'. Mature fruits are dry and have been used as pain relievers. Other names include evening hoehyang, sohoehyang, earthhhoehyang, hyangjasohyang, and so on, and the seeds of fennel are sometimes referred to as mountain parsley seeds.

In the cosmetic composition according to the present invention, the clove is an evergreen tree belonging to the Cloveaceae family, and is called clove in the West, and has been used as one of the representative potions since ancient times. When they met the emperor, they held a clove in their mouth to get rid of bad breath, and this was called Gyesolhyang. In general, the buds of cloves have been used as herbal medicinal herbs (clove, 丁香), and are known to have anticonvulsant, antidepressant, nervous system soothing, and sterilization effects.

In the cosmetic composition according to the present invention, the fennel extract and the cloves extract may be extracted from seeds of fennel and cloves, respectively.

The extract according to the present invention can be used to separate and obtain an extract using a method known in the art for extraction and separation, and the extract defined in the present invention is an active ingredient prepared by the above method using an appropriate solvent. The raw material can be extracted as it is or by grinding. At this time, any suitable solvent that can be used to obtain the extract may be used as long as it is an acceptable solvent in the art, and water or an organic solvent may be used. For example, alcohol having 1 to 4 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether , Benzene, chloroform, ethyl acetate, methylene chloride, hexane, and various solvents such as cyclohexane may be used alone or in combination. It is not limited.

Preferably, the extract may be extracted using at least one extraction solvent selected from the group consisting of water, an anhydrous or water-containing lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent, or a mixed solvent thereof, More preferably, it may be extracted using one or more extraction solvents selected from water or ethanol.

As the extraction method, any one of methods such as hot water extraction, cold precipitation extraction, reflux cooling extraction, solvent extraction, steam distillation method, ultrasonic extraction method, elution method, and compression method may be used. In addition, the desired extract may be further subjected to a conventional fractionation process, or may be purified using a conventional purification method. There is no limitation on the method for preparing the extract of the present invention, and any known method may be used.

For example, the extract contained in the composition of the present invention may be used as it is while the primary extract extracted by the above-described solvent extraction method is centrifuged, filtered, concentrated, freeze-dried, and stored in a refrigerator. It can be prepared in powder form by additional processes such as distillation and freeze drying or spray drying. Further, the primary extract was further purified by using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get

Therefore, in the present invention, the extract is a concept including all extracts, fractions and purified products obtained in each step of extraction, fractionation, or purification, their dilutions, concentrates, or dried products.

In an embodiment of the present invention, the cosmetic composition may include 0.1-2.0 parts by weight of sericin, 1.0-3.0 parts by weight of fennel extract, 1.0-3.0 parts by weight of clove extract, based on 1 part by weight of the epidermal growth factor, Preferably, 0.5-1.5 parts by weight of sericin, 1.5-2.5 parts by weight of fennel extract, 1.5-3.5 parts by weight of clove extract based on 1 part by weight of the epidermal growth factor may be used.

In one embodiment of the present invention, the cosmetic composition for preventing skin aging due to oxidative stress may be one to prevent skin aging through an antioxidant effect.

In one embodiment of the present invention, the cosmetic composition for preventing or improving skin wrinkles may prevent or improve skin wrinkles by increasing collagen production and inhibiting elastase activity.

The cosmetic composition according to the present invention includes serum, lotion, nutrition lotion, nutrition cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, foundation, makeup base, soap, cleanser, oil, wax and It may be one or more formulations selected from the group consisting of sprays, and preferably used in a serum form. Other ingredients other than the above essential ingredients may be appropriately selected and blended by a person skilled in the art without difficulty according to other formulations or purposes of use.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and/or bis. It may be provided in the form of a warm vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of an adjuvant that can be applied topically or systemically used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventional in the field. It can be manufactured according to the method.

In addition, in addition to the epidermal growth factor, sericin, fennel extract and clove extract of the present invention, the cosmetic composition may contain additional fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents ( foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives , Lipid vesicles or any other ingredients commonly used in cosmetics, such as adjuvants commonly used in the cosmetic field. These adjuvants are introduced in amounts commonly used in the field of cosmetology.

In one embodiment of the present invention, in addition to the above ingredients, the cosmetic composition includes glycerin, hyaluronic acid solution, silk amino acid solution, vitamin E, niacinamide, adenosine, olive liquid, daisy oil, tea tree oil, nenoly oil, lemon oil, camellia It may further include one or more components selected from the group consisting of oil, 1,2-hexanediol, and neroli water. At this time, glycerin 7.0-9.0 parts by weight, hyaluronic acid solution 9.0-11.0 parts by weight, silk amino acid solution 14.0-16.0 parts by weight, vitamin E 2.0-4.0 parts by weight, niacinamide 0.1-2.0 parts by weight based on 1 part by weight of the epidermal growth factor , 0.1-2.0 parts by weight of adenosine, 2.0-4.0 parts by weight of olive liquid, 0.2-0.3 parts by weight of daisy oil, 0.01-0.1 parts by weight of tea tree oil, 0.01-0.1 parts by weight of neroli oil, 0.01-0.1 parts by weight of lemon oil, camellia It may contain 4.0-6.0 parts by weight of oil, 1.0-3.0 parts by weight of 1,2-hexanediol, and 35 to 55 parts by weight of neroli water, but is not limited thereto.

Hereinafter, the present invention will be described in more detail by examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited to these examples.

< Manufacturing example 1> Preparation of fennel seed extract

Dried and ground fennel ( foeniculum) vulgare ) seed 10g was added with 500ml of 50% ethanol as an extraction solvent, and extracted in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 70℃ for 6-12 hours to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh) to obtain a fennel seed filtrate. After removing the solvent from the filtrate using a rotary evaporator (DL10-3000, Greatwall, 20L), fennel seed extract was prepared. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

< Manufacturing example 2> cloves Preparation of seed extract

To 10 g of dried and crushed clove ( Eugenia c aryophyllus ) seeds, 500 ml of 70% ethanol was added as an extraction solvent, and extracted in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) at 70° C. for 6 to 12 hours to obtain an extract. The extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 4 to 400 mesh) to obtain a filtrate of cloves seeds. After removing the solvent from the filtrate using a rotary evaporator (DL10-3000, Greatwall, 20L), a clove seed extract was prepared. The extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored refrigerated at 5°C.

< Example 1 and Comparative example 1-7> EGF , Sericin, fennel seed extract and cloves Containing seed extract Serum Produce

First, for EGF (Epidermal Growth Factor), Human Oligopeptide-1 (Nano EGF (Epidermal Growth Factor/1ppm)) was purchased from Lohas Nature Co., Ltd. (Daegu Metropolitan City, Korea). And sericin (sericin) was purchased and used from pincredit bio-tech co., ltd (Xi'an city, China).

A serum composition containing EGF, sericin, fennel seed extract (Preparation Example 1) and clove seed extract (Preparation Example 2) was prepared in the compositions of Tables 1 and 2 below. At this time, in Tables 1 and 2 below, the content of the remaining components is expressed in parts by weight based on 100 parts by weight of the total composition.

(Part by weight) EGF Sericin Fennel seeds
extract Cloves seeds
extract Example 1 1.0 1.0 2.0 2.0 Comparative Example 1 1.0 1.0 - - Comparative Example 2 1.0 - 2.0 - Comparative Example 3 1.0 - - 2.0 Comparative Example 4 1.0 1.0 2.0 - Comparative Example 5 1.0 1.0 - 2.0 Comparative Example 6 1.0 - 2.0 2.0 Comparative Example 7 1.0 - - -

Other ingredients other than the active ingredients in Table 1 are as shown in Table 2 below.

Other ingredients Parts by weight Glycerin 8.00 Hyaluronic acid 10.00 Silk Amino Acids 15.00 Vitamin E (Tocopherol) 3.00 Niacinamide 1.00 Adenosine 1.00 Olive Liquid (INCI: Sodium PEG-7 Olive Oil Carboxylate) 3.00 Daisy Oil 0.10 Tea Tree Essential Oil 0.07 Neroli Essential Oil 0.07 Lemon Essential Oil 0.07 Camellia Essential Oil 5.00 1,2-hexanediol (1,2-Hexanediol) 2.00 Neroli water Balance

< Experimental example 1> Evaluation of antioxidant efficacy

In order to confirm the antioxidant effect of Example 1 and Comparative Examples 1 to 7, L-Ascorbic acid was used as a comparative sample, and DPPH radical scavenging ability was measured using the DPPH method.

Specifically, the DPPH solution was a 0.2 mM solution of 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl free radical (Aldrich Chem Co, MW=618.76), dissolved in methanol and adjusted to 100 ml. To a 96-well plate, 0.15 ml of a 0.2 mM DPPH solution and 0.15 ml of a sample solution (Example 1 or Comparative Examples 1 to 7) were added, stirred rapidly, and incubated at 25° C. for 10 minutes, and the absorbance (St) at 560 nm was measured. Measured. The absorbance (Bt) was measured in the same manner as above using distilled water instead of the sample solution as a control, and the blank of the sample solution was absorbance (Bo) in the same manner using methanol instead of the 0.2 mM DPPH solution. Was measured. At this time, each sample solution was treated at a concentration of 100 ppm.

DPPH radical scavenging ability was calculated by Equation 1 below, and the results are shown in Table 3 below.

[Equation 1]

DPPH radical scavenging rate (%)={1-(St-So)/(Bt-Bo)}×100

(St: absorbance at 560 nm after free radical scavenging of the sample solution,

Bt: absorbance at 560 nm after free radical scavenging of the control solution,

So: absorbance at 560 nm before reaction when free radicals are not added to the sample solution,

Bo: Absorbance at 560 nm before reaction when free radicals were not added to the control solution.)

ingredient Erasure rate (%) Example 1 EGF+sericin+fennel+clove 94.62 Comparative Example 1 EGF+sericin 9.59 Comparative Example 2 EGF+fennel 11.41 Comparative Example 3 EGF+clove 10.85 Comparative Example 4 EGF+sericin+fennel 15.02 Comparative Example 5 EGF+sericin+clove 16.36 Comparative Example 6 EGF+fennel+clove 18.94 Comparative Example 7 EGF 6.92

As shown in Table 3, when one or more of EGF and sericin, fennel seed extract, and clove seed extract were mixed compared to Comparative Example 7 containing EGF alone, it was found that the DPPH radical scavenging rate was increased, and in particular, EGF , Sericin, fennel seed extract, and clove seed extract were all mixed in Example 1, the DPPH radical scavenging ability was the best, it was confirmed that the antioxidant effect is excellent.

< Experimental example 2> Elastase Inhibitory activity evaluation

In order to confirm the effect of inhibiting skin aging and wrinkles in Example 1 and Comparative Examples 1 to 7, the inhibitory activity of elastase, an elastin-degrading enzyme, was measured.

Using N-succinyl-(L-Ala)3-p-nitroanilide, S4760, Sigma) as a substrate, the amount of p-nitroanilide produced from the substrate was measured at 445 nm for 20 minutes at 37°C. That is, the serum composition of Example 1 and Comparative Examples 1 to 7 was prepared as a test solution to a concentration of 200 ppm, and 500 μL was taken into a test tube, and porcine pancreas elastase (E1250, Sigma) dissolved in 2 mM tris-HCl buffer (pH 86). 2.5U/mL) solution was added, and then N-succinyl-(L-Ala)3-p-nitroanilide (500μg/mL) dissolved in 50mM tris-HCl buffer (pH 8.6) was added as a substrate and reacted for 20 minutes. I did. Elastase inhibitory activity was calculated according to the following Equation 2 as the absorbance reduction rate of the sample solution added group and the non-added group, and shown in Table 4.

[Equation 2]

Elastase inhibition rate (%) = {(absorbance of no added group-absorbance of sample added group)/absorbance of no added group}×100

ingredient Elastase inhibitory activity (%) Example 1 EGF+sericin+fennel+clove 74.25 Comparative Example 1 EGF+sericin 26.41 Comparative Example 2 EGF+fennel 28.02 Comparative Example 3 EGF+clove 27.36 Comparative Example 4 EGF+sericin+fennel 30.84 Comparative Example 5 EGF+sericin+clove 31.93 Comparative Example 6 EGF+fennel+clove 32.64 Comparative Example 7 EGF 24.22

As shown in Table 4, when one or more of EGF and sericin, fennel seed extract, and clove seed extract were mixed compared to Comparative Example 7 containing EGF alone, the elastase inhibition rate was found to increase, but Comparative Example In the case of 1 to 6, while not increasing to a significant level, it was confirmed that the elastase inhibitory activity was the best in Example 1 in which EGF, sericin, fennel seed extract and clove seed extract were all mixed.

< Experimental example 3> Evaluation of collagen production efficacy

Collagen is synthesized as procollagen within the cell, then secreted to the outside of the cell and polymerized into collagen fibers. Such collagen is a structural protein constituting the skin, and when the content of collagen in the skin is high, when the activity of collagen-degrading enzyme is low, wrinkles of the skin are improved. The method of measuring such collagen is a Procollagen Type I C-Peptide (PIP) EIA kit using human procollagen type I C-terminal peptide (PIP) secreted to the outside of the cell using human dermal fibroblasts. And it was confirmed the collagen production amount (%) compared to the non-treated group according to the treatment of Comparative Examples 1 to 7.

ingredient 100ppm 200ppm 500ppm Example 1 EGF+sericin+fennel+clove 109.87% 116.42% 128.85% Comparative Example 1 EGF+sericin 103.42% 105.19% 110.23% Comparative Example 2 EGF+fennel 104.55% 106.12% 111.19% Comparative Example 3 EGF+clove 104.85% 107.50% 110.64% Comparative Example 4 EGF+sericin+fennel 106.52% 109.24% 112.67% Comparative Example 5 EGF+sericin+clove 107.16% 110.14% 113.25% Comparative Example 6 EGF+fennel+clove 106.24% 109.23% 114.52% Comparative Example 7 EGF 102.12% 104.85% 109.23%

As a result, as shown in Table 5, when one or more of EGF, sericin, fennel seed extract, and cloves seed extract were treated compared to the amount of collagen produced in the untreated group (100%), the amount of collagen produced increased by 2.12 to 28.85%. appear. At this time, compared to Comparative Examples 1 to 7 in which EGF alone or sericin, fennel seed extract and some of clove seed extract were combined, the effect of increasing collagen production in Example 1 in which all of EGF, sericin, fennel seed extract, and clove seed extract were mixed is It was the most excellent, and it was confirmed that the wrinkle improvement effect was excellent.

< Experimental example 4> Evaluation of skin wrinkle and elasticity improvement efficacy

After dividing the group into 10 people per evaluation group targeting women in their 30s to 50s who do not have skin allergy symptoms, the serum prepared in Example 1 and Comparative Examples 1 to 7 of the present invention was applied once a day for 8 weeks. The skin condition before and after 8 weeks of use was evaluated, and the average was calculated to evaluate the effect of improving skin wrinkles and elasticity.

The evaluation criteria used a scale of 1-5, and the skin condition for skin wrinkles and skin elasticity was 1 point for'very bad', 2 points for'slightly worse', 3 points for'no change', and'slight improvement' '4 points for'and 5 points for'very improved'. The experimental results are shown in Table 6 below. The presence or absence of skin irritation was evaluated as (number of persons who showed irritation reaction)/(total number of test subjects).

Skin wrinkles Skin elasticity Presence of skin irritation Example 1 4.8 4.7 0/10 Comparative Example 1 3.7 3.6 2/10 Comparative Example 2 3.8 3.7 1/10 Comparative Example 3 3.7 3.6 1/10 Comparative Example 4 3.9 4.0 1/10 Comparative Example 5 4.0 3.9 0/10 Comparative Example 6 4.1 3.9 0/10 Comparative Example 7 3.5 3.4 2/10

As shown in Table 6, when using the serum of Example 1 in which all four types were mixed than the case of using the serum of Comparative Examples 1 to 7 containing only some combinations of EGF, sericin, fennel seed extract and clove seed extract It was confirmed that the skin wrinkle improvement and skin elasticity improvement effect were more excellent.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (5)

Epidermal growth factor (EGF); And
Sericin (sericin), fennel ( Foeniculum vulgare ) extract and cloves ( Eugenia caryophyllus ),
A cosmetic composition for preventing skin aging due to oxidative stress, comprising 0.1-2.0 parts by weight of sericin, 1.0-3.0 parts by weight of fennel extract, and 1.0-3.0 parts by weight of clove extract based on 1.0 parts by weight of the epidermal growth factor.

Epidermal growth factor (EGF); And
Sericin (sericin), fennel ( Foeniculum vulgare ) extract and cloves ( Eugenia caryophyllus ),
A cosmetic composition for preventing or improving skin wrinkles, comprising 0.1-2.0 parts by weight of sericin, 1.0-3.0 parts by weight of fennel extract, and 1.0-3.0 parts by weight of clove extract based on 1.0 parts by weight of the epidermal growth factor.
The method according to claim 1 or 2,
The cosmetic composition, characterized in that the fennel extract and the cloves extract are extracted from seeds of fennel and cloves, respectively.
delete The method according to claim 1 or 2,
The cosmetic composition is composed of serum, lotion, nutrition lotion, nutrition cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, foundation, makeup base, soap, cleanser, oil, wax and spray. Cosmetic composition, characterized in that at least one formulation selected from the group.