KR101477126B1 - Cosmetic composition containing korean natural medical-herbal extracts with the whitening and antioxidant effect - Google Patents

Abstract

The present invention relates to a cosmetic composition containing choleric esophageal acid, which is excellent in whitening, antioxidant and anti-inflammatory effect, and is characterized by comprising at least one compound selected from the group consisting of clove, sandalwood, ginseng, oriental, citrus, fennel, And chrysanthemum baculovirus including herbal extracts comprising chrysanthemum bark extract. The cosmetic composition according to the present invention protects the skin from various stresses, smoothes and softens the skin, inhibits skin aging, And anti-inflammatory effects.

Whitening effect, antioxidant effect, anti-inflammatory effect, cosmetics,

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a chrysanthemal bacillus acid having excellent whitening, antioxidant and anti-inflammatory effects,

The present invention relates to a cosmetic composition containing a chrysanthemum baculovan acid which is excellent in whitening, antioxidant and anti-inflammatory effects, and more particularly, The present invention relates to a cosmetic composition containing a chylomorpholinic acid and a chylomorpholinic acid, which are prepared by efficiently extracting an active ingredient from Baekjong, Baek, The present invention relates to a cosmetic composition having a whitening effect, an antioxidative effect and an anti-inflammatory effect by inhibiting aging.

Generally, there are two kinds of skin aging phenomenon, natural aging (intrinsic aging) and aging (or exogenous aging) caused by ultraviolet light, which are generally started from 25 to 26 years old. Natural aging is an inevitable phenomenon that can not be avoided with age, and it can not be avoided through gene transfer, which is known to be caused by active oxygen generated during the breathing process. As age increases, the elasticity of the skin gradually decreases, and the amount of moisture and sebum also decreases greatly after 30s. In the case of sebum, it decreases continuously after the age of 20, and the decrease of the moisture amount and the sebum amount greatly influences the wrinkle formation of the skin after the 20 th to 30 th generation.

Another aging phenomenon, photo aging (extrinsic aging), means aging that occurs because the skin is always exposed to the external environment. Due to the use of cooling and heating devices, it is difficult to maintain proper moisture of the skin due to a dry indoor environment, and it is difficult for the skin to maintain a normal water content by wind or chemical substances. In particular, extrinsic aging is caused by exposure of the skin to ultraviolet rays. Therefore, it is a big difference from endogenous aging that it can be avoided considerably.

The main cause of photo aging is known as active oxygen generated when sunlight (ultraviolet rays) is received. The ultraviolet rays contained in the sunlight are divided into long wavelength, medium wavelength and short wavelength depending on the wavelength range, and it is known to cause various side effects to the skin. Particularly, ultraviolet rays of ultraviolet rays are very harmful to the human body but up to now, they are absorbed into the ozone layer in the atmosphere and hardly reach to the surface of the earth. Therefore, long wavelength ultraviolet rays and medium ultraviolet rays cause side effects. Long wavelength ultraviolet rays cause pigmentation, phototoxicity, light allergy and the like, and medium-wave ultraviolet rays having a larger energy cause erythema, blistering, burn, skin cancer, pigmentation and skin protein denaturation. Since blocking ultraviolet rays is very important for the health of the skin, conventionally, various ultraviolet blocking agents have been developed to prevent ultraviolet rays.

Conventional ultraviolet absorbers include chemical substances such as benzophenone derivatives, paraamino derivatives, cinnamic acid derivatives and triazine derivatives. As ultraviolet scattering agents, inorganic pigments such as titanium dioxide and zinc oxide have been used. The ultraviolet absorber absorbs ultraviolet rays mainly in the mid-wave ultraviolet region and is a chemical synthetic substance itself, so there are many possible side effects such as irritation and allergy to the skin. In addition, since ultraviolet scattering agents affect not only ultraviolet rays but also visible rays, they have been pointed out that they are opaque when used as cosmetics and have a bad feeling when applied to skin.

On the other hand, a substance having an ultraviolet absorbing function separated and extracted from a natural product is generally considered to be promising as an ultraviolet absorber to be incorporated in cosmetics since it has less irritation and has other pharmacological actions such as anti-inflammation, astringent action and antibacterial action , There has been an attempt to search for and isolate ultraviolet absorbing substances contained in natural products.

In order to overcome the disadvantages of the known ultraviolet screening agents and to develop a better ultraviolet screening agent to overcome the above problems, the present inventors have efficiently extracted the active ingredients from various natural herbal medicines through a low temperature ultrasonic extraction method While looking for a way to do it, the natural herbal ingredient, cloves, consisting of clove, sandalwood, ginseng, oriental, janghong, fennel, kwakyoung, Baekbokryeong, white wax, white peony, white wax, white ginseng, And has a whitening effect, an antioxidative effect and an anti-inflammatory effect. Thus, the present invention has been completed.

It is an object of the present invention to overcome the disadvantages of the known ultraviolet screening agents and to provide a better ultraviolet screening agent by efficiently extracting an effective ingredient from natural herbal medicines through a low temperature ultrasonic extraction method to provide a whitening effect, To provide an excellent cosmetic composition.

In order to accomplish the above object, the present invention provides a method of producing a herbal medicine comprising a herbal medicine extract comprising clove, sandalwood, ginseng, persimmon, citrus, fennel, woogeolyeo, white ginseng, And a cosmetic composition which is excellent in whitening, antioxidant and anti-inflammatory effects.

1 to 30% by weight of clove, 1 to 30% by weight of sandalwood, 1 to 30% by weight of persimmon bran, 1 to 30% by weight of anchovy, 1 to 30% by weight of citronella, 1 to 30% by weight of fennel 1 1 to 30% by weight of white bauxite, 1 to 30% by weight of Baekbokgol, 1 to 30% by weight of Baekwang, 1 to 30% by weight of Baekjang, 1 to 30% 30 to 1% by weight of a mixture of a lower alcohol and ethanol as an organic solvent in a ratio of 1: 1 to 5: 1 And then ultrasonic extraction is carried out.

The cosmetic composition of the present invention preferably contains 0.1 to 10.0% by weight of the pyrotaloic acid produced as described above, and may be formulated into a lotion, essence, lotion, cream, gel or ointment formulation.

According to the present invention, natural low-temperature ultrasound extraction methods are used to minimize the loss of effective ingredients from cloves, sandalwood, sandalwood, gypsum, oriental, janghong, fennel, The cosmetic composition of the present invention, which contains herbal extracts as a raw material, is used to protect skin from various sensitive stresses, to provide skin with a smooth and radiant texture, to inhibit skin aging and to provide whitening, antioxidative and anti-inflammatory effects do.

In order to provide a cosmetic composition having an ultraviolet shielding effect and a whitening, antioxidant and anti-inflammatory effect, the present invention contains a quercetinic acid composed of natural herbal ingredients. Here, in order to maximize the extraction efficiency of natural herbal medicine components, a low-temperature ultrasonic method was applied. Low-temperature ultrasonic extraction was performed using 1,3-butylene glycol, propylene glycol, ethanol or purified water alone or in combination, Has a high absorbance and is excellent in solubility, affinity and stability for various bases, additives and organic solvents for cosmetics, and particularly provides a synergistic effect of significantly increasing the ultraviolet barrier property when used in combination with a conventional ultraviolet screening agent.

The raw materials for preparing the herbal medicine extracts used in the present invention are selected from the group consisting of cloves, sandalwood, ginseng, oriental, janghong, fennel, hwakulyeong, white ginseng, white peach, white peach, white ginseng, 1 to 30% by weight of clove, 1 to 30% by weight of sandalwood, 1 to 30% by weight of sorghum, 1 to 30% by weight of citrus, 1 to 30% by weight of citrus fruit, 1 to 30% by weight of fennel, 1 to 30% by weight of white baux, 1 to 30% by weight of white baux, 1 to 30% by weight of baux, 1 to 30% by weight of baux, 1 to 30% by weight of white baux, 1 to 30% %.

The mixture consisting of these cloves, sandalwood, persimmon, persimmon, citrus, fennel, woogeol, Baekbokryeong, white rye, white peony, white liquor, white liquor, white liquorice and white liquor is subjected to a drying process, Lt; / RTI > for 1 hour. Such a wet heat treatment step lowers the microbial contamination degree and forms a chemical condition in which the nutrients of the ingredients constituting each mixture are activated to increase the extraction efficiency. Subsequently, purified water and a lower alcohol as an organic solvent, especially ethanol, are mixed at a mixing ratio of 1: 1 to 5, and the mixture is stirred using a low-temperature ultrasonic extraction method. For further purification, low molecular weight substances can be removed by dialysis using a membrane filter with a molecular weight of 10 kD at 4 ° C. The same volume of distilled water as the volume of the separated solution is added to lower the viscosity of the extract, followed by lyophilization or spray drying. The chilcorpolinic acid produced by this method is added at the time of cosmetic preparation.

In the present invention, a chilled antioxidant is prepared by using a low-temperature ultrasonic extraction method in order to improve the extraction efficiency and the stability of the active ingredient of the hot water extraction by immersion, which is a conservative herbal medicine extraction method. In the production method of the present invention, low temperature means 60 ° C or less. This low temperature extraction minimizes the effect on the compositional changes of herbal medicine extracts that may occur during extraction. The ultrasound extraction method according to the present invention stabilizes the unstable structure of various useful components, and the ultrasound of a wide range penetrates deeply into the plant material, thereby effectively extracting an effective ingredient of the natural product.

In the ultrasonic wave extraction method according to the present invention, ultrasound refers to a sound of a high frequency that can not be heard by the human ear. The ultrasound extraction method emits ultrasonic waves having a frequency of 20 KHz or higher to the extracting material and the extraction solvent, thereby generating high pressure due to the joint effect of the ultrasonic waves, thereby breaking down the internal tissues and effectively extracting the active ingredient Technology. Such an ultrasonic extraction method is disclosed in detail in, for example, Journal of the Korean Society for Biotechnology, Vol. 19, No. 2, 113-117 (2004).

The advantage of the ultrasonic extraction method used in the preparation of the present invention is that the extraction efficiency is very high and the possibility of denaturation and destruction of the active ingredient exhibiting pharmacological activity can be minimized. In addition, since the extract production speed is faster than the conventional hot water extraction method, the extraction time is shortened and the use of low-cost low energy is thus obtained.

The herbal anti-chrysanthemum extract of the present invention can be added to cosmetic preparations by adding an organic solvent containing 1 to 5 times of ethanol or butylene glycol or purified water.

When the chrysanthemum halobacterium according to the present invention is used in a cosmetic composition, the natural extract composed of clove, sandalwood, gherkin, oriental jelly, fennel, fennel, woogeulhyeong, white ginseng, white peony, white ginseng, white ginseng, By weight to 10% by weight, preferably 0.1 to 5% by weight. If the amount of the natural extract is less than 0.1% by weight, the skin protection effect against ultraviolet rays is insufficient when added, and if it is more than 10% by weight, the inherent odor and color depending on the inherent flavor of the extract in the final product are strong.

The cosmetic composition containing the chylomorpholinic acid having a whitening, antioxidant and anti-inflammatory effect according to the present invention may have conventional formulations. For example, cosmetic compositions such as softening lotion, convergent lotion, nutritional lotion, essence such as body essence, A lotion such as a body lotion, a cream, a gel or an ointment formulation such as a nutritional cream. The composition of each of these formulations may contain appropriate various bases and additives necessary for the formulation of the formulation, and the kind and amount of these components can be easily selected by the manufacturer.

In addition, the cosmetic composition containing chlordetra arbutic acid having whitening, antioxidant, and anti-inflammatory effects according to the present invention can be used according to a conventional method of use, and may be used in a different number of times depending on the skin condition or taste of the user.

Hereinafter, the cosmetic composition containing the chylomorpholoxane having the whitening, antioxidant and anti-inflammatory effects of the present invention will be described in more detail by way of examples. However, these examples are merely examples of the present invention, and the scope of the present invention is not limited thereto

Example  One: Seventy-five percent  Produce

The raw materials of dried cloves, sandalwood, ginseng, oriental, citrus, fennel, kwakwoong, Baekbokryeong, white, white peony, white, white,

The natural herbal medicine raw material mixture composed of clove, sandalwood, persimmon, persimmon, citrus fruit, fennel, woogeolyeo, white ginseng, white peony, white peony, white ginseng, white ginseng and white mugwort was subjected to wet heat treatment at 80 to 100 ° C for 30 minutes to 1 hour Respectively. Purified water and a lower alcohol as an organic solvent, especially ethanol, were mixed at a mixing ratio of 1: 1, and the mixture was stirred using an ultrasonic extraction method. The extract was recovered, concentrated, and then purified. If further purification is desired, low molecular weight substances can be removed by dialysis using a membrane filter with a molecular weight of 10 kD at 4 ° C. Productivity was determined by adding the same volume of distilled water to the volume of the separated solution to reduce the viscosity of the chilled peppermint bauxic acid, lyophilization and measuring the dry weight.

The mixture was extracted with low temperature ultrasonication and then concentrated under reduced pressure to obtain a mixture of 2 g of fuller's arm and 10 g of fuller's milk. Acetic acid.

Hereinafter, representative skin lotions and milk lotions of a cosmetic composition having a skin protecting effect against ultraviolet rays will be described as an example.

Example  2: Seven shavings  Included skin  Manufacture of lotions

Skin lotions were prepared at the composition ratios shown in Table 1 below.

The alcohol portion was dissolved in the order of p-oxybenzoic acid ester, tween 20, and NP-12 in the alcohol produced in the curd and distilled at 78 ° C, and the purified water portion was dissolved in sterilized purified water in the order of glycerin, 1,3-butylene glycol, Phosphoric acid, hyaluronic acid, allantoin, citric acid, and sodium citrate were mixed in this order to prepare a skin lotion.

Classification Raw material name Weight (% by weight) Alcohol fraction ethanol 7.0 Paraoxybenzoic acid ester 0.1 tween-20 0.2 Nonyl phenyl ether-12 0.2 Purified water portion Purified water 89.51 glycerin 0.1 1,3-butylene glycol 0.2 Betaine 0.1 Hyaluronic acid 0.5 Allantoin 0.05 Citric acid 0.01 Sodium citrate 0.03 additive Seven White Sharks 2.0 Sum 100

Example  3: Seven shavings  Included milk  Manufacture of lotions

Milk lotion was prepared in the same manner as in Example 2, according to the composition ratios shown in Table 2. [

Raw material name Weight (% by weight) Purified water Balance Triethanolamine 0.3 Carboxyvinyl polymer 0.2 Concentrated glycerin 5.0 Propylene glycol 5.0 Paraoxybenzoic acid ester 0.2 Cetostearyl alcohol 0.8 Glyceryl monostearate 1.5 Sorbitan monostearate 0.3 Fio (20) Sorbitan monostearate 1.5 Isocetyl myristate 7.0 Isocetyl octanoate 5.0 Spices 0.16 Seven White Sharks 2.0 Sum 100

Experimental Example  1: According to the present invention skin  Identify skin irritation and protective effect of lotion

A predetermined amount (10 mg / 5 cm 2) of the skin lotion prepared in Example 2 was applied to the forehead portions of 40 healthy male and female adults (20 men and 20 women), and the other portions were covered with aluminum foil Minute, a long wavelength ultraviolet ray was irradiated at a dose of 10.2 J / cm 2 using an ultraviolet ray irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours.

As a result of the observation, it was confirmed that the skin lotion according to the present invention has no phototoxicity and has a skin protection effect because no adverse effects such as erythema have occurred in the skin.

Experimental Example  2: According to the invention milk  Identify skin irritation and protective effect of lotion

A predetermined amount (10 mg / 5 cm 2) of the milk lotion prepared in Example 3 was applied to the forehead portions of 40 healthy male and female adults (20 men and 20 women), and the other portions were covered with aluminum foil Minute, a long wavelength ultraviolet ray was irradiated at a dose of 10.2 J / cm 2 using an ultraviolet ray irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours.

As a result of the observation, it was confirmed that the milk lotion according to the present invention has no phototoxicity and has a skin protection effect because side effects such as erythema were not generated on the skin.

Experimental Example  3: According to the present invention Seventy-five percent  Check skin stability

In order to compare the safety of a sample containing 2.0% of chrysanthemum bisulphate, 1.0% of sodium lauryl sulfate and 10% of ethanol to 1.0% of sodium lauryl sulfate and 10% of ethanol, Human patch test) was used for the first stimulation test.

The human skin patch test was conducted in 50 healthy adult men and women according to the CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, DC, 20036, 1991). 20 μl of sample and solution was dropped into a Finn chamber, and this was placed on the back of the human body to be tested and fixed with a tape. After 24 hours, the patches were removed. After 4 hours, the skin reaction of the test site was judged according to the following criteria, and the results are shown in Table 3.

<Criteria>

-; No erythema or unusual symptoms

±; Slightly reddish than around

+; Remarkably reddened than surrounding

++; More reddened and swollen than around

The number of subjects to be tested is expressed by the following formula: Number of stimuli = [{(占) number 占 1} + {占 number 占 2} + {

sample Number of subjects Judgment result Irritation degree ++ + ± - (3)
Sodium lauryl sulfate (1%) and
Ethanol (10%) 50 - - 5 45 0.10 Sodium lauryl sulfate (1%) 50 2 8 12 28 0.68 Ethanol (10%) 50 - - 10 40 0.20

Experimental Example  4: Seventy-five percent  UV-cut effect according to wavelength

Ultraviolet rays are classified into long UV-A and relatively short UV-B. In the case of UV-A, the dermis reacts strongly to reach the deep skin and darkens the skin. In the case of UV-B, it causes sunburn on the skin and promotes pigmentation. In order to block such ultraviolet rays, a sunscreen agent is used, and the skin barrier itself may induce skin irritation.

In the present experiment, it was determined whether or not skin damage could be prevented by measuring the ultraviolet blocking effect according to the wavelength of the heptaflyaval acid prepared in Example 1 using 40% of 1,3-butylene glycol and 0.2% of phenonip Respectively.

FIG. 1 is a graph showing the ultraviolet blocking effect according to the wavelength of a chrysanthemum bacillus acid according to the present invention. From this, it was confirmed that the present invention can absorb both UV-A and UV-B to lower the possibility of skin irritation.

Experimental Example  5: Seventy-five percent DPPH  Antioxidant effect by scavenging activity

Skin aging processes by active oxygen include lipid peroxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal cross-linking. These reactions are mostly radical reactions involving an autoxidation process. The antioxidant plays a role in blocking the autoxidation reaction in vivo, and the stronger the reducing power is, the stronger the antioxidant is. A reagent for measuring the reducing power of an antioxidant is 1,1-diphenyl-2-picrylhydrazyl radical (DDPH). DPPH exists as a radical in the stabilized structure of the nitrogen center in the compound, which shows maximum absorption at 517 nm and disappears at 517 nm when reduced. The reducing power of the chiculosalbacanic acid according to the present invention is represented by the scavenging activity (SC 50), and the SC 50 represents the concentration required to reduce the concentration of DPPH by 50%.

In the present experiment, the SC50 of each concentration of chilipatholic acid according to the present invention was measured. FIG. 2 is a graph showing the DPPH scavenging activity according to the concentration of chlordate arginate according to the present invention, and the following Table 4 shows the DPPH scavenging activity (SC 50) according to the present invention in comparison with ascorbic acid .

DPPH scavenging activity (SC 50) Seven White Sharks 1.52 [mu] l / ml Ascorbic acid 2.20 [mu] l / ml

As shown in FIG. 2 and Table 4, it can be confirmed that DPPH scavenging activity is increased in proportion to the concentration of pale yellow alpaca acid according to the present invention, and that it has an antioxidative effect superior to ascorbic acid.

Experimental Example  6: Seventy-five percent Hydroxyl Radical  Antioxidant effect by scavenging activity

Hydroxyl radicals are the most potent free radicals that damage cells. They are known to be active oxygen species that cause peroxidation of skin cell membrane lipids, especially those that participate in DNA and connective tissue damage. In particular, superoxide and hydrogen peroxide are produced by the photo-sensitization reaction, which begins with ultraviolet absorption, and they participate in the generation of more dangerous hydroxyl radicals in vivo. That is, the superoxide can reduce the iron ion and the copper ion, and the reduced iron and copper reduce the hydrogen peroxide and consequently produce the hydroxyl radical.

In this experiment, the ability of chalcophtalic acid according to the present invention to cleave hydroxyl radicals produced by Fenton reaction in vitro was measured. FIG. 3 is a graph showing the hydroxyl radical scavenging activity according to the concentration of choline pyrophosphate acid according to the present invention, and the following Table 5 shows the hydroxyl radical scavenging activity (SC 50) of choline pyrophosphate according to the present invention compared with Trolox .

Hydroxyl radical scavenging activity (SC 50) Seven White Sharks 145.9 [mu] l / ml Trolox 926.1 [mu] l / ml

As shown in FIGS. 3 and 5, hydroxyl radical scavenging activity was increased in proportion to the concentration of chlorophyll acid according to the present invention, and SC 50 was found to be 145.9 ㎕ / ml, which is superior to Trolox.

Experimental Example  7: Seventy-five percent  Depending on concentration Superoxide  Anion-scavenging activity effect

Among the active oxygen, the superoxide anion is produced by a photo-sensitization reaction that starts with ultraviolet absorption, and is converted to hydrogen peroxide by a superoxide dismutase (SOD) enzyme present in vivo. Superoxide and hydrogen peroxide are weakly reactive and rarely participate directly in tissue cell damage, but participate in the production of more dangerous hydroxyl radicals. That is, superoxide can reduce iron and copper ions, and reduced iron and copper ions reduce hydrogen peroxide, resulting in hydroxyl radicals. In addition, in vivo, superoxide can release iron ions (II) from ferritin. This phenomenon appears after UV irradiation and is considered to be the action of superoxide generated by ultraviolet rays.

In this experiment, the superoxide radical scavenging activity (SC 50) according to the concentration of the chrysanthemum baux acid according to the present invention was measured. FIG. 4 is a graph showing the superoxide radical scavenging activity according to the concentration of the chrysanthematobacillus acid according to the present invention, and the following Table 6 shows a comparison of the superoxide radical scavenging activity (SC 50) .

Superoxide radical scavenging activity (SC 50) Hibiya 0.7 [mu] l / ml Trolox 27.2 [mu] l / ml

As shown in FIGS. 4 and 6, the supernatant of the present invention exhibits a superoxide radical scavenging activity in proportion to the concentration, and SC 50 is 0.7 μl / ml, which is superior to Trolox.

Experimental Example  8: Seventy-five percent  Depending on concentration Collagenase  By active inhibition Anti-wrinkle  effect

One of the causes of skin wrinkles is skin collagen, a deficiency of collagen. Collagen is the main protein that constitutes the skin dermis and plays a role in keeping skin structure and elasticity. It is known that the collagen is reduced in its age and its degree of degradation increases with age, inducing skin dermal layer depression to produce skin wrinkles. In addition, MMP (matrix metalloproteinase) such as collagenase is expressed by ultraviolet rays which is a cause of photoaging, and decomposition of collagen is thereby increased to generate wrinkles.

In this experiment, it was confirmed whether or not the chlorthophylla acid according to the present invention inhibited collagenase activity, thereby confirming the effect of improving the wrinkles of the skin. 0.05 ml of the substrate solution in which 0.2 mM FALGPA was dissolved and 0.05 ml of the sample solution were added to 0.8 ml of 0.05 M tricine buffer (pH 7.5) as a reaction solution, 0.05 ml of collagenase (3 mg / ml) And the mixture was allowed to stand at 20 占 폚 for 18 hours. Thereafter, 10 μl of 1 N acetate was added to stop the reaction, and the absorbance at 324 nm was measured to evaluate the collagenase inhibitory activity.

FIG. 5 is a graph showing the collagenase inhibitory activity according to the concentration of chilipatholic acid according to the present invention, and Table 7 shows the collagenase inhibitory activity (IC 50) of chilphedargalic acid according to the present invention.

Collagenase inhibitory activity (IC 50) Seven White Sharks 1.38 [mu] l / ml

 As shown in FIG. 5 and FIG. 7, the collagenase inhibitory activity and the collagenase inhibitory activity (IC 50) of the present invention were excellent as 1.38 μl / ml.

Experimental Example  9: Seventy-five percent  Depending on concentration Tyrosinase  Whitening effect by active inhibition

(No. JK, et al ., Life Science , 65, p241-246, 1999), in order to confirm the tyrosinase activity inhibitory effect of the choline- . As a control group of the chilled antiprotonic acid obtained in Example 1, a hot water extraction method using water heated at 100 캜 as an extraction solvent was applied for the same time to prepare a sample.

Specifically, 1 ml of a 0.3 mg / ml tyrosine solution was put into a test tube, and 1 ml of 0.1 M potassium phosphate buffer solution (pH 6.8) and 0.5 ml of a sample (0.5, 1.0, 2.0, 4.0% v)) was added thereto, and the mixture was allowed to react in a 25 ° C thermostat for 10 minutes. At this time, as a control group, 0.9 ml of a solvent was used instead of each sample. To the reaction solution was added 0.1 ml of 2500 unit / ml of tyrosinase enzyme, and the mixture was reacted in a 25 ° C thermostat for 10 minutes. The reaction tube was quenched by placing the test tube with the reaction solution in ice water, and the absorbance at 475 nm was measured with a photoelectric spectrometer.

FIG. 6 is a graph showing tyrosinase-inhibiting activity according to the concentration of chlordate arginate in accordance with the present invention, and Table 8 shows the tyrosinase inhibitory activity (IC 50) of chiller arginate in comparison with kojic acid.

Tyrosinase inhibitory activity (IC 50) Seven White Sharks 2.11 [mu] l / ml Kojic acid 15.7 [mu] g / ml

As shown in FIG. 6 and Table 8, the inhibitory effect of tyrosinase inhibitory activity of the quercetin monoacidic acid was 20 to 90% at concentrations of 0.06, 0.13, 0.25, 0.5, 1.0 and 2.0 (%, v / v) , IC 50 was 2.11 쨉 l / ml, which was superior to kojic acid.

Experimental Example  10: Seventy-five percent  Anti-inflammatory effect by concentration

In order to investigate the anti-inflammatory effect of chylomorphic bacillus acid, evaluation with arachidonic acid (A. Crummey, GP Harper, EA Boyle and FR Mangan. Inhibition of arachidonic acid- induced ear edema as a model for assessing topical anti- Agents and Actions 1987: 20: 69-76).

Thirty-five mice (hairless mice) were cleaned with ethanol before each sample, and the thickness of both ears was measured using a micrometer. Then, 0.5% and 1.0% ) 1.0% treated group, and control group treated with arachidonic acid alone, and the average value of mouse ear thickness was measured. Twenty microliters of the sample and 20 microliters of ethanol were continuously applied to the control group and the indomethacin-treated group for 1 day, respectively, for 4 days. One hour after the last application, ethanol was applied to the left side and arachidonic acid was applied to the right ear at 2 mg / ear. After 1 hour of application, the degree of eeema of the ear was measured three times And repeatedly measured. The left ear of the treated group was used as a control group to examine the degree of inflammation induced by the sample itself. The anti-inflammatory effect was evaluated based on the control group treated with arachidonic acid alone. The results are shown in Table 9. &lt; tb &gt; &lt; TABLE &gt;

sample density(%) Glume (㎛) % Inhibition Before sample treatment Sample treatment water Sample group Seven White Sharks 0.5 323 536 22.3 Seven White Sharks 1.0 320 495 36.1 Comparative group Indomethacin 1.0 327 468 48.5 Control group Arachidonic acid 2 mg / ear 324 598

※ Inhibition rate (%) = (AB) / A × 100

A: Mean thickness change of the control ear

(Thickness of ear treated with arachidonic acid - thickness of untreated ear)

B: Mean thickness change of sample application group ear

(Thickness of sample treated ear - thickness of untreated ear)

As shown in Table 9, the anti-inflammatory effect of chrysanthemum Bacillus subtilis showed a high anti-inflammatory effect of 36.1%, which is about 75% of the anti-inflammatory drug indomethacin . In addition, when verifying the significant difference in the rate of edema growth, the effect of having a significant difference of 99% with arachidonic acid was shown.

Hereinafter, the following preparations were prepared by using the chitosan-resistant white bean which is excellent in anti-inflammatory and anti-irritating effect and safe to the skin.

Manufacturing example  1: Lotion

According to the following formulation, it was prepared according to the conventional lotion preparation method.

Raw material name Weight% (w / w) glycerin
Dipropylene glycol
Hyaluronic acid
Polyoxyethylene hardened castor oil
Polyethylene oleyl ethyl
ethanol
antiseptic
Spices
Pigment
Seven White Sharks
Purified water 5.0
3.0
0.5
0.1
0.1
5.0
0.15
Suitable amount
Suitable amount
2.0
to 100

Manufacturing example  2: essence

According to the following prescription, it was prepared according to a conventional method for producing essence.

Raw material name Weight% (w / w) Cetostearyl alcohol
Self emulsifying monostearic acid
Wax
Squalane
Isocetyl octanoate
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Carboxy polymer
Triethanolamine
antiseptic
Spices
Pigment
Seven White Sharks
Purified water 1.0
1.0
0.5
5.0
3.0
0.3
0.5
8.0
4.0
0.2
0.22
0.25
Suitable amount
Suitable amount
Suitable amount
7.0
to 100

Manufacturing example  3: Lotion

According to the following formulation, it was prepared according to a conventional lotion preparation method.

Raw material name Weight% (w / w) Cetostearyl alcohol
Self emulsifying monostearic acid
Wax
Stearic acid
Liquid paraffin
Squalane
Macadamia oil
Isocetyl octanoate
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Betaine
Carboxy polymer
Triethanolamine
antiseptic
Spices
Pigment
Seven White Sharks
Purified water 0.8
1.0
0.5
0.5
7.0
5.0
3.0
2.0
0.3
0.5
1.2
4.0
4.0
4.0
0.12
0.15
0.25
Suitable amount
Suitable amount
5.0
to 100

Manufacturing example  4: Cream

According to the following formulation, it was prepared according to a conventional cream production method.

Raw material name Weight% (w / w) Cetostearyl alcohol
Self emulsifying monostearic acid
Chin type monostearate
Wax
Liquid paraffin
Squalane
Isocetyl octanoate
Refined jojoba oil
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Betaine
Xanthan gum
Triethanolamine
antiseptic
Spices
Pigment
Seven White Sharks
Purified water 3.0
1.5
1.5
0.5
8.0
7.0
4.0
4.0
0.3
1.0
1.2
6.0
4.0
4.0
0.06
0.10
0.25
Suitable amount
Suitable amount
7.0
to 100

Manufacturing example  5: Gel

According to the following prescription, it was prepared according to a conventional gel preparation method.

Raw material name Weight% (w / w) glycerin
Propylene glycol
ethanol
Polyoxyethylene hardened castor oil
Carboxy polymer
Triethanolamine
antiseptic
Spices
Pigment
Seven White Sharks
Purified water 4.0
4.0
10
0.1
0.30
0.30
Suitable amount
Suitable amount
Suitable amount
1.0
to 100

Manufacturing example  6: Ointment

The ointment was prepared according to the conventional ointment manufacturing method according to the following prescription.

Raw material name Weight% (w / w) Cetostearyl alcohol
Self emulsifying monostearic acid
Stearic acid
Wax
Squalane
Monostearin glycerin
Sorbitan monostearate
Polysorbate 80
glycerin
Propylene glycol
Spices
Pigment
Seven White Sharks
Vaseline 2.0
2.0
1.0
4.0
7.0
3.0
1.0
3.0
5.0
4.0
Suitable amount
Suitable amount
10.0
to 100

FIG. 1 is a graph showing the ultraviolet blocking effect according to the wavelength of a chrysanthemum bacillus acid according to the present invention.

FIG. 2 is a graph showing DPPH scavenging activity according to the concentration of heptachloric acid in accordance with the present invention. FIG.

FIG. 3 is a graph showing the hydroxyl radical scavenging activity according to the concentration of choline pyrophosphoric acid according to the present invention.

FIG. 4 is a graph showing the superoxide radical scavenging activity according to the concentration of the heptafarbox acid according to the present invention. FIG.

FIG. 5 is a graph showing collagenase inhibitory activity according to the concentration of chilipatholic acid according to the present invention. FIG.

FIG. 6 is a graph showing tyrosinase inhibitory activity according to the concentration of chilipathol Baic acid according to the present invention.

Claims (3)

Antioxidant and antioxidative substances characterized by comprising a chrysanthemum bauxite composed of herbal extracts extracted from cloves, sandalwood, sandalwood, ginseng, oriental, janghonghwa, fennel, hwajeobyeong, white ginseng, white ginseng, white peony, white ginseng, white ginseng, A cosmetic composition excellent in anti-inflammatory effect. The composition of claim 1, wherein the paleoic acid is present in an amount of from 1 to 30% by weight of clove, from 1 to 30% by weight of sandalwood, from 1 to 30% by weight of persimmon, from 1 to 30% 1 to 30% by weight of fennel, 1 to 30% by weight of Baekbokryeol, 1 to 30% by weight of Baekboksil, 1 to 30% by weight of Baekwu, 1 to 30% 1 to 30% by weight and 1 to 30% by weight of Bacillus cereus is wet-treated at 80 to 100 ° C for 30 minutes to 1 hour, purified water and lower alcohol are added at a volume ratio of 1: 1 to 5, &Lt; / RTI &gt; The cosmetic composition according to claim 1, wherein the composition contains 0.1 to 10.0% by weight of chrysanthematophilic acid.

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