KR100881001B1 - Cosmetic composition containing natural complex extracts with the effect of antioxidant, whitening and anti-inflammation - Google Patents

Abstract

The present invention is a natural complex extract excellent in antioxidant, whitening and anti-inflammatory effects, natural complex consisting of extracts consisting of situation mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms and extracts consisting of fresh ginseng, red ginseng, black ginseng and Gaza extract The present invention relates to a cosmetic composition containing an extract, and more particularly, each extract is extracted by ultrasonic extraction instead of general hydrothermal extraction, and the bioactive substance is selectively enriched than when the bioactive component is extracted by general hydrothermal extraction. By selectively fractional extraction of relatively necessary functional ingredients to provide an excellent cosmetic composition that finally exhibits a physiological function such as excellent antioxidant effect, whitening effect and anti-inflammatory effect in a small amount.

Antioxidant, Situation Mushroom, Ganoderma Lucidum Mushroom, Cosmetics

Description

Cosmetic composition containing natural complex extracts with the effect of antioxidant, whitening and anti-inflammation}

1 is a graph showing the inhibitory effect of tyrosinase activity of the natural complex extract prepared by the present invention,

Figure 2 is a graph showing the antioxidant effect by DPPH scavenging ability of the natural complex extract prepared by the present invention,

3 is a graph showing the antioxidant effect by the superoxide anion elimination activity of the natural complex extract prepared by the present invention,

Figure 4 is a graph showing the antioxidant effect of the hydroxyl radical (hydroxyl radical) scavenging activity of the natural complex extract prepared by the present invention,

5 is a graph showing the anti-inflammatory effect according to the inhibitory ability of lipoxygenase of the natural complex extract prepared by the present invention

The present invention is a natural complex extract excellent in antioxidant, whitening and anti-inflammatory effects, natural complex consisting of extracts consisting of situation mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms and extracts consisting of fresh ginseng, red ginseng, black ginseng and Gaza extract The present invention relates to a cosmetic composition containing an extract, and more specifically, an extract consisting of green mushrooms, ganoderma lucidum and matsutake mushrooms constituting a natural complex extract, and an extract consisting of fresh ginseng, red ginseng, and black ginseng, and Gaza extract. The extraction process is improved by the ultrasonic extraction method, not the extraction method, so that the biologically active ingredient is selectively richer than the case extracted by the normal hot water extraction, so as to exhibit excellent functions, and the natural complex having excellent antioxidant, whitening effect and anti-inflammatory effect. Providing a cosmetic composition containing an extract The.

Generally, ultraviolet rays included in the sun's rays are divided into long, medium, and short wavelengths according to their wavelength ranges. These ultraviolet rays are known to cause various side effects on the skin.

Short wavelength ultraviolet rays in ultraviolet light are very harmful to the human body, but until now they are absorbed by the ozone layer in the atmosphere and hardly reach the earth's surface. Medium wavelength ultraviolet rays, which cause photoallergy and have higher energy, cause erythema, blistering, burns, skin cancer, pigmentation, and skin aging. Importantly, various sunscreens have been developed to block ultraviolet rays.

Conventional ultraviolet absorbers include compounds such as benzophenone derivatives, paraamino derivatives, cinnamic acid derivatives and triazine derivatives, and inorganic pigments such as titanium dioxide and zinc oxide have been used as ultraviolet scatterers. It absorbs only the ultraviolet rays of the intestinal ultraviolet region and is a chemical synthetic substance, which is likely to cause other side effects on the skin, such as irritation and allergy. In addition, UV scattering agent is opaque when used as a cosmetic in addition to ultraviolet light as well as visible light has been pointed out that the feeling is poor when applied to the skin.

On the other hand, a substance having an ultraviolet absorbing effect extracted from a natural product generally has a low irritation, and has other pharmacological effects such as anti-inflammatory, astringent, and antimicrobial effects. Efforts have been made to search for and isolate ultraviolet absorbers contained in natural products.

In addition to the screening and efficacy evaluation of bioactive substances in natural products, a review of the manufacturing process should be supported. Nevertheless, studies on the process of producing high content extracts for physiologically active substances contained in natural products have not been found at home and abroad.

Currently, extraction and separation processes that can be applied to produce bioactive substances from natural products can be classified into chemical methods, physical methods, and enzymatic methods. Among these various extraction methods, extraction methods that are commonly used today may be called chemical methods in terms of productivity and quality control. However, most chemical extraction methods use organic solvents for extraction conditions that take a long time at high temperatures, especially hours and days, so that new alternative processes are urgently needed in terms of working environment, safety, and time. .

The present invention is to solve the above problems, as a part of the research to overcome the disadvantages of the known sunscreen and to develop a better sunscreen using a conventional water extraction method using purified water or organic solvents from various natural products Ultrasonic extraction was used as a method to overcome the extraction efficiency and the limitation of solvent use, which were limited from the extraction by the solvent extraction method. This resulted from the characteristics of herbal extracts. Natural complex extract with excellent antioxidant, whitening and anti-inflammatory effects during the study of complex extract, consisting of extracts consisting of mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms, and extracts consisting of fresh ginseng, red ginseng, black ginseng and Gaza extract Natural Complex Extraction It is an object to provide a cosmetic composition containing water.

The present invention is a natural complex extract excellent in antioxidant, whitening and anti-inflammatory effects, natural complex consisting of extracts consisting of situation mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms and extracts consisting of fresh ginseng, red ginseng, black ginseng and Gaza extract The present invention relates to a cosmetic composition containing an extract, and more particularly, to extract each of the extracts constituting the natural complex extract by the ultrasonic extraction method, thereby improving the extraction process. Compared with the extract extracted by the extraction method, it has an excellent antioxidant effect, whitening effect and anti-inflammatory effect, has a high specific absorbency, good solubility, affinity and stability for various bases and additives for cosmetics, organic solvents, etc. Sunscreen when used in combination with sunscreen The effect that the significant increase in protecting the skin from various stress by providing synergy cultivated skin moist and clean to provide excellent cosmetic composition.

The method for producing a natural complex extract of the present invention uses a low-temperature ultrasonic extraction method that improves the extraction efficiency of the extraction by immersion, more specifically, the ultrasonic extraction method used in the present invention is pulverized, mixed and As a method applied in order to obtain good efficiency in a fast reaction time in the extraction process, the conventional extraction method, the hydrothermal extraction method has a lower extraction efficiency, more energy consumption and many useful components due to heat than the ultrasonic extraction method used in the present invention Has the disadvantages of destruction and loss of protein, heat denaturation of protein, and heat instability. On the other hand, the ultrasonic extraction method has the advantage of increasing the efficiency of the extraction process and safely and effectively obtaining many bioactive substances when applied to herbs or natural herbal raw materials.In the ultrasonic extraction method, ultrasonic waves are sound (sound waves) and the human ear. Refers to the sound of high frequency which cannot be heard, where low temperature means below 60 ℃, and the situation mushrooms are generally designed to avoid the extraction temperature of high or cryogenic level to selectively remove unnecessary components and It was aimed at selective reinforcement and overcomes the disadvantages of inadequate selectivity of solvent by ultrasonic extraction method. In other words, efforts have been made to minimize the effect of changes in the composition of natural complex extracts that may occur during extraction, and this ultrasonic extraction method stabilizes the unstable structure of various useful components and effectively penetrates plant raw materials by a wide range of ultrasonic waves to effectively treat natural products. It has the advantage of extracting the ingredients effectively.

Advantages of the ultrasonic extraction method used in the preparation of the natural complex extract of the present invention has the feature of controlling the intensity of the ultrasonic energy change depending on the raw material to be extracted. Therefore, the extraction efficiency is very high depending on the raw materials, and the possibility of denaturation and destruction of active ingredients exhibiting pharmacological activity is minimized, and the extraction time is shortened because the production speed is faster than that of the conventional hydrothermal extraction method, which results in low cost and low energy use. It was confirmed to have.

Extracts used in the natural complex extract of the present invention is composed of extracts consisting of situation mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms, and extracts composed of fresh ginseng, red ginseng, black ginseng and Gaza extract.

The raw material of each extract used in the preparation of the natural complex extract of the present invention, the raw material is first washed with purified water, and then finely pulverized, and 1 to 10% by weight of the pulverized product, in addition to purified water, polyol or ethanol, respectively 1 to 50 The active ingredient of the natural complex extract may be modified and extracted by dipping into 1% to 5 days by weight, preferably 3 days, and then using a low temperature ultrasonic extraction method for 1 to 5 hours. At this time, by selecting one of polyol, ethanol, or a mixed solvent thereof, 10 to 70% by weight, 10 to 40% by weight of purified water may be mixed and used as a mixed solvent to be extracted by ultrasonic method.
That is, each group is divided into the raw material of the mushroom system consisting of the situation mushroom, ganoderma lucidum, and pine mushroom constituting the natural complex extract of the present invention, and the raw material of the three lines consisting of fresh ginseng, red ginseng, and black ginseng and Gaza raw materials. In addition to each of the polyol or ethanol or their mixed solvent as an extraction solvent for the extraction solvent to perform the immersion step and the ultrasonic extraction step to finally extract and use each extract mixed. Therefore, the sedimentation reaction due to interference by different raw materials is prevented in the extraction step of each subdivided raw materials.

One or more of hexylene glycol, dipropylene glycol, and propylene glycol can be selected and used as a polyol from the said extraction solvent.

An organic solvent or purified water containing 1 to 5 times of ethanol or butylene glycol is added to the natural complex extract mixed at the above compounding ratio and added when preparing cosmetics.

When the natural complex extract according to the present invention is used in the cosmetic composition, it is characterized in that it contains 0.1 to 50% by weight, preferably 2 to 10% by weight.

At this time, less than 0.1% by weight of the skin protection effect and moisturizing elasticity of the present invention is insufficient when added, and when used more than 50% by weight of the unique flavor and color according to the unique flavor in the final product is strong Difficulty in product application, more preferably limited to 2 to 10% by weight.

Cosmetic composition having a skin calming effect and moisturizing elasticity against ultraviolet rays according to the present invention may have a conventional formulation, for example, supple cosmetics, facial essence (mask), nutrition lotion, nutrition cream, sunblock cream, body Lotions, body essences, and the like. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Hereinafter, a representative facial essence and a nourishing cream composition of a cosmetic composition having a skin protection effect and a moisturizing elasticity function against ultraviolet rays will be described.

In addition, the natural complex extract excellent in the antioxidant and whitening effect and anti-inflammatory effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

Hereinafter, the cosmetic composition containing the natural complex extract excellent in the antioxidant effect, the whitening effect, and the anti-inflammatory effect of the present invention will be described in more detail with reference to the examples, but the present invention is not limited to these examples.

Example 1 Preparation of Natural Complex Extract

Extract consisting of green mushroom, ganoderma lucidum mushroom and pine mushroom and extract consisting of fresh ginseng, red ginseng and black ginseng and black ginseng extract dried dried green mushrooms, ganoderma lucidum, pine mushroom, fresh ginseng, red ginseng, black ginseng, and Gaza Used.

Raw mushrooms, ganoderma lucidum mushroom, pine mushroom, ginseng, red ginseng, black ginseng, and Gaza raw materials constituting the natural complex extract of the present invention are first washed with purified water, and then grouped mushrooms, ganoderma lucidum and pine mushrooms. Ginseng, red ginseng and black ginseng are grouped separately, and the Gaza is finely ground separately, and the grouped raw materials are used at a level of 20 g / L for 1 hour using 1 to 10 wt% of the extraction solvent based on the ground solids content for each group. Immersion in purified water to maximize the extraction of the active ingredient of the extract for each grouped raw material using the ultrasonic extraction method at 25 ℃ of low temperature for 3 hours.
That is, each group is divided into the raw material of the mushroom system consisting of the situation mushroom, ganoderma lucidum, and pine mushroom constituting the natural complex extract of the present invention, and the raw material of the three lines consisting of fresh ginseng, red ginseng, and black ginseng and Gaza raw materials. Select one of polyol, ethanol or polyol and ethanol mixed solvent as an extraction solvent, and use the mixture at a predetermined ratio with purified water to perform an immersion step and an ultrasonic extraction step to finally extract and mix each extract to apply a concentration step. do. Therefore, the sedimentation reaction due to interference by different raw materials is prevented in the extraction step of each subdivided raw materials.

As a natural complex extract excellent in antioxidant, whitening and anti-inflammatory effects according to the present invention, it is composed of extracts consisting of green mushrooms, ganoderma lucidum mushrooms, matsutake mushrooms, and extracts consisting of fresh ginseng, red ginseng, black ginseng and Gaza extract As a method of producing a natural complex extract, purified water was selected as a natural complex extracting solvent, and the extraction solvent ratio of the complex sample was constant, and extraction experiments were performed at room temperature using ultrasonic power and extraction time per unit volume of the extraction solution as process and system variables. Ultrasonic generators were used for high power operating at 20 kHz and 400, 1200, 2400, 4000 W / L were applied using high power ultrasonic generators.

In the method for producing a natural complex extract excellent in the antioxidant effect, the whitening effect and the anti-inflammatory effect according to the present invention, it was confirmed that the extraction effect of the pharmacologically active ingredient according to the sonication time was weak when using the low power ultrasonic generator. In the preparation method of the natural complex extract excellent in the antioxidant effect, the whitening effect and the anti-inflammatory effect, the output of 2400W / L for high power was applied. The reason for this was that the yield of the natural complex extract of the present invention was not remarkably decreased at the output of 1100 to 2400 W / L, and the yield of the natural complex extract was the best. Above 2400 W / L, the extraction yield did not increase significantly. Therefore, in the method of producing a complex extract according to the present invention, by treating high-power ultrasound for 3 hours, the best effective yield was obtained at an output of 1200 to 2400 W / L. In addition, the effective yield decreased as the ratio of purified water increased as the extraction solvent. That is, when using purified water as the extraction solvent of the natural complex extract according to the present invention, the appropriate range is 50, g / L range of 10, 20, 30, 50, 70, 100 g / L (natural complex / purified water ratio) of the preliminary experiment When it is passed, the effective yield is confirmed to be lowered to the level of 60% of 20g / L. Therefore, when using purified water as the extraction solvent, the effective range was found to be the most suitable 20 ~ 50g / L. In this case, when the purified water was used at less than 20 g / L, the amount of the complex sample per unit volume of the extraction solvent was added in an excessive amount, thereby confirming that the concentration effect of the complex extract was greatly reduced.

As shown in Table 1, the extract consisting of the situation mushroom, Ganoderma lucidum mushroom, pine mushroom, and complex extract consisting of the extract consisting of ginseng, red ginseng, black ginseng extract and Gaza extract is dried mushrooms, ganoderma lucidum mushroom, pine mushroom , Ginseng, red ginseng, black ginseng, and Gaza raw materials are grouped into low-temperature ultrasonic extraction, and then filtered. Each of the grouped raw material extracts is collected and synthesized into a final complex extract. Natural complex extract consisting of ginseng, red ginseng, black ginseng, and Gaza was obtained.

Weight before extraction (g) Weight after extraction (g) 10.0 2.0

The method of producing a natural complex extract according to the present invention reacts only for a few minutes at room temperature when a little ultrasonic wave is applied to extract a physiologically active substance from natural products such as situation mushrooms, ganoderma lucidum mushrooms, pine mushrooms, ginseng, red ginseng, black ginseng, and Gaza Even if it is using only the organic solvent was extracted preliminary results excellent extraction effect than when extracted for 30 minutes at 60 ℃. However, a control group was prepared as a hydrothermal extraction method for more objective evaluation in the method of preparing an extract consisting of situation mushroom, ganoderma lucidum mushroom, pine mushroom, ginseng, red ginseng, black ginseng, and Gaza according to the present invention. That is, as a control for the natural complex extract according to the present invention, 10 g of the raw material was first washed with purified water, finely pulverized, and 1 to 10% by weight of the pulverized product was immersed in purified water for 1 hour to extract purified water as an extract solvent for 3 hours. 2g of the control sample was prepared using a hydrothermal extraction method as the extraction temperature of 100 ℃.

Example 2 Preparation of Facial Essence with Skin Protection and Elasticity Effect Containing Natural Complex Extract According to the Present Invention

To prepare a facial essence in the composition ratio as shown in Table 2. The manufacturing method includes the steps of adding and dissolving the purified water portion to 60 ° C purified water, adding and dissolving the moisturizing elastic component dissolved at 40 ° C, and cooling and then adding and dissolving the functional cosmetic ingredient at 30 ° C. And it was prepared by aging and then mixed with the natural complex extract prepared in Example 1 to prepare a facial essence.

Classification Raw material name Weight (% by weight) Purified water Purified water 65.53 glycerin 7.0 Dipotassium glycyrinate 0.1 Disodium ID 0.05 Hydroxyethyl Cellulose 0.05 Dipropylene glycol 3.0 Moisturizing elasticity part Methylparaben 0.1 Eichparaben 0.1 Tocopherol Acetate 0.02 Squalane 0.05 Polyoxyethylene HydroxyCaster Oil 1.1 Sodium Pie 1.0 Functional material part Witch Hazel Extract 1.0 Sodium hyaluronate 0.5 Collagen 0.2 Aloe extract 0.2 Natural Complex Extract 20.0 Sum 100.00

Example 3: Preparation of a nourishing cream having a skin protection effect and a whitening function containing a natural complex extract according to the present invention

Dissolving the purified water portion by heating it to 80 ° C., emulsifying by adding the dissolved oily part heated to 80 ° C., adding and mixing a thickener dissolved in warm water at 60 ° C., and cooling and adding and mixing cosmetic ingredients. Cooling to 30 ℃ and defoaming, performing the step of filtration and aging prepared at 50 ℃ to prepare a nourishing cream.

In addition, when the natural complex extract is used in the cosmetic composition, the prepared natural complex extract is characterized in that it contains 0.1 to 50% by weight, preferably 2 to 10% by weight.

Classification Raw material name Weight (% by weight) Purified water Purified water 52.36 Propylene glycol 5.0 glycerin 6.0 Xanthan Gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 Natural Complex Extract 10.0 Oily ingredients Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl Monostearate 1.0 Microcrystalline Wax 0.6 Fiji-100 0.3 Tocopherol Acetate 1.0 Shea butter 1.0 Propylparaben 0.1 Oryzanol 0.1 Bisabolol 0.2 Dimethicone 0.5 Monostearic acid polyoxyethylene sorbitan 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredients Sodium ascorbate 0.1 Phospholipids 1.0 Placenta Extract 0.5 Aloe extract 0.5 Tocopherol Acetate 0.5 Apple extract 1.0 Sum 100.00

At this time, when less than 0.1% by weight, the antioxidant effect, the whitening effect, and the anti-inflammatory effect of the present invention are insignificant, and when used more than 50% by weight, the unique product and strong odor according to the intrinsic flavor in the final product are strongly applied. There is a difficulty in more preferably limited to 2 to 10% by weight.

Experimental Example 1: Confirmation of skin irritation, skin protection and elasticity effect of facial essence containing natural complex extract according to the present invention

30 minutes after applying the facial essence prepared in Example 2 (10m / 5cm2) to the forearm of 30 healthy men and women (15 males, 15 females), and shielding the other parts with aluminum foil Long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, it was confirmed that there was no phototoxicity and skin protection effect because no side effects such as erythema did not occur on the skin, and 60 healthy men and women (30 males and 30 females) were divided into two groups. Example 2 was prepared without the experimental group coated with a predetermined amount (10m / 5cm2) of the prepared facial essence and the natural complex extract according to the present invention to control the skin elasticity 10mm in diameter after 1 hour, 3 hours, 6 hours Compared to the time when the mark disappeared by giving a force of about 5 Newtons with a hollow cylindrical structure consisting of a frame, it was found to disappear from the experimental group at about 20% level for each hour compared to the control group. It was confirmed that the skin elasticity is improved by the use of the essence.

Experimental Example 2: Confirmation of skin irritation and protective effect of nutrition cream containing natural complex extract according to the present invention

30 minutes after applying the nutrition cream prepared in Example 3 (10m / 5cm2) to the forearm of 30 healthy men and women (15 males and 15 females), and shielding the other parts with aluminum foil Long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, no side effects such as erythema occurred on the skin, and thus the nourishing cream had no phototoxicity and had a skin protective effect. In addition, 60 healthy men and women (30 males, 30 females) were divided into two groups, and the experimental group was applied to the nutritional cream prepared in Example 3 by a predetermined amount (10m / 5cm2) and the complex extract according to the present invention. 1 hour, 3 hours, and 6 hours after the preparation of Example 3, the skin elasticity was a hollow cylinder consisting of a 10 mm diameter border, and the strength of about 5 Newtons was applied to determine the disappearance time. Compared with each time, it disappears from the experimental group at about 17% level, and it was confirmed that the skin elasticity was improved by the use of the nourishing cream containing the natural complex extract of the present invention.

Experimental Example 3: Skin stability confirmation test of facial essence and nutrition cream according to the present invention

A human patch test was carried out on the forearm of 30 healthy adult men and women (15 males and 15 females) using the facial essences and nutrition creams prepared in Examples 2 and 3 above. Wipe the forearm of the subject well with 70% ethanol, and then place a pin chamber containing 20 μl of the facial essence and nutrition cream prepared in Examples 2 and 3 on a conventional cosmetic base using an adhesive tape for 24 hours. Closed attachment. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. Experimental results As shown in Table 4, the human patch test to confirm the skin irritation of the facial essence and nutrient cream according to the present invention did not show a positive response was confirmed that there is no irritation to the human skin.

sample After 24 hours (n = 30) After 48 hours (n = 30) Remarks Facial essence 0/30 0/30 Number of reaction quantums / total number of targets Nutrition cream 0/30 0/30

Experimental Example 4 Inhibitory Effect of Tyrosinase Activity According to Concentration of Natural Complex Extract of the Present Invention

In order to confirm the inhibitory effect of tyrosinase activity using the extract obtained in Example 1, it was measured by modifying the method described in the literature, such as No. JK, et al .; Life Science , 65, p241-246, 1999 ). A sample of the control group was prepared by using a hydrothermal extraction method using water heated at 100 ° C. as the same extraction solvent at the same time as a control group of the method of preparing the extract obtained in Example 1.

Figure 1 is a graph showing the inhibitory effect of tyrosinase activity of the natural complex extract prepared by the present invention, which will be described in more detail, first 0.3 mg / ml tyrosine solution in 1 ml each test tube, 0.1M potassium phosphate 1 ml of buffer solution (pH 6.8) and 0.9 ml of sample according to the concentration of the extract (0.5, 1.0, 2.0, 4.0 (%, V / V)) were added and reacted for 10 minutes in a 25 ° C thermostat. In this case, only 0.9 ml of the solvent was used instead of each sample as a control. 0.1 ml each of 2500 units / ml of tyrosinase enzyme was added to the reaction solution, and then reacted in a 25 ° C. thermostat for 10 minutes.

The test tube containing the reaction solution was placed in iced water, quenched to stop the reaction, and the absorbance at 475 nm was measured with a photoelectric spectrometer. As shown in FIG. 1, the inhibitory effect of tyrosinase activity of the samples was 12% and 25% for the hydrothermal extract at 2.0 and 4.0 (%, V / V), respectively, and 25% and 43% for the ultrasonic extract. In addition, it was confirmed that the extract extracted by using ultrasonic waves showed a higher effect than the inhibitory effect of tyrosinase activity of the extract by the hot water extraction method.

Experimental Example 5: Antioxidative Effect of Natural Complex Extracts by DPPH Scavenging Ability

Samples of the control group and the experimental group of the natural complex extract was prepared in the same manner as in Experiment 4.

Skin aging processes with free radicals include lipid peroxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal crosslinks. Most of these reactions are radical reactions that involve an automatic oxidation process. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the reducing power as an electron-giving ability, the stronger the antioxidant.

A reagent that measures the reducing power of antioxidants is 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. Maximum absorption at 517 nm is shown and when reduced, absorption is lost at 517 nm. The magnitude of the reducing power of the natural complex extract of the present invention is represented by the radical scavenging activity (SCcavenging activity, SC50) and SC50 represents the concentration required to reduce the concentration of DPPH by 50%, Figure 2 is a natural complex extract prepared by the present invention As a graph showing the antioxidant effect of the DPPH scavenging ability of the extract, it will be described in more detail, showing the results obtained by measuring the SC50 for each concentration of the natural complex extract, the concentration of the extract 0.0004, 0.0008, 0.0017, 0.0033, 0.0067 In (%, V / V), the hydrothermal extract represents 1%, 1%, 10%, 21%, 46%, and the ultrasonic extract represents 9%, 9%, 19%, 43%, 78%, Antioxidant effect of the extract extracted by using ultrasonic wave was higher than that of the extract by the extraction method.

Experimental Example 6: Antioxidant Effect of Superoxide Anion Scavenging Activity of Natural Complex Extracts

Samples of the control group and the experimental group of the natural complex extract was prepared in the same manner as in Experiment 4. Superoxide anion scavenging activity was measured by modifying Noro et al. using superoxide anion generation system by xanthine / xanthine oxidase enzyme reaction.

The change of absorbance due to oxidation of NBT due to superoxide anion produced by xathine / xanthine oxidase enzyme reaction was measured. Add 0.05 M Na2CO3, 3 mM xanthine, 3 mM EDTA, 0.15% BSA, 0.75 M NBT and sample, mix well, and let stand at 25 ° C for 10 minutes, add xanthine oxidase and react at 25 ° C for 20 minutes The reaction was stopped to measure the absorbance at 560 nm. Scavenging activity of superoxide anion was calculated according to the following equation.

* Superoxide anion scavenging activity (%) = [1- (A-B) / (C-B)] × 100

A: Absorbance of sample at 560 nm

B: Absorbance of blank at 560 nm

C: Absorbance of control at 560 nm

Figure 3 is a graph showing the antioxidant effect of the superoxide anion scavenging activity according to the concentration of the natural complex extract prepared by the present invention, it will be described in more detail,

At the concentrations of the extracts 0001, 0.002, 0.003 and 0.007 (%, V / V), the hydrothermal extracts showed 1%, 21%, 43% and 64%, and the ultrasonic extracts showed 18%, 34%, 83% and 87%. It was expressed as%, and the antioxidative effect of the superoxide anion scavenging activity was higher in the extract extracted using ultrasonic wave than the antioxidative effect of the superoxide anion scavenging activity of the extract by the hydrothermal extraction method.

Experimental Example 7: Antioxidative Effect of Natural Complex Extracts by Hydroxy radical Scavenging Activity

Samples of the control group and the experimental group of the natural complex extract was prepared in the same manner as in Experiment 4. Hydroxyl radicals are the most powerful free radicals that damage cells and cause free radical peroxidation of the skin cell membranes, especially reactive oxygen species, which are the major contributors to DNA and connective tissue matrix damage. In particular, hydroxy radicals and hydrogen peroxide are produced by the photosensitization reaction that begins with ultraviolet absorption, and they participate in the production of more dangerous hydroxyl radicals in vivo. That is, superoxide can reduce Fe (III) and Cu (II), and reduced Fe (II) and Cu (I) reduce hydrogen peroxide and consequently produce hydroxyl radical. This can be assessed by measuring the ability to eliminate hydroxyl radicals generated by the Fenton reaction in vitro.

Figure 4 is a graph showing the antioxidant effect of the hydroxy radical scavenging activity of the natural complex extract prepared by the present invention, when described in more detail, the concentration of the extract 0.1, 0.2, 0.5 (%, V / V) The hot water extracts represent 12%, 27%, 58%, and the ultrasonic extracts represent 42%, 49%, and 84%. Ultrasonic extracts use ultrasonic waves rather than the antioxidant effect of the hydroxy radical scavenging activity of the extract by the hot water extraction method The extracted extract showed antioxidant effect by higher hydroxy radical scavenging activity.

Experimental Example 8: Anti-inflammatory Effect of Natural Complex Extracts According to Inhibitory Activity of Lipoxygenase

Samples of the control group and the experimental group of the natural complex extract was prepared in the same manner as in Experiment 4. Lipoxygenase (5-LO, 5-lipoxygenase) adds molecular oxygen to unsaturated fatty acids with 1,4-cis, cis-pentadiene (1,4-pentadiene) structures in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. It is an oxidase that produces 1,3-cis, trans-diene 5-hydroperoxide. It is the enzyme involved in the first reaction of leucotriene biosynthesis from arachidonic acid, which is involved in various inflammatory and allergic diseases. Therefore, anti-inflammatory effect can be evaluated by measuring inhibitory activity of lipoxygenase using SLO (soybean lipoxygenase) similar to 5-LO structurally and mechanism.

5 is a graph showing the anti-inflammatory effect according to the inhibitory ability of lipoxygenase of the natural complex extract prepared by the present invention, which will be described in more detail.

The concentrations of the extracts were 0.03, 0.05, 0.13, 0.25 (%, V / V), respectively, and the hydrothermal extracts represented 1%, 2%, 10%, and 22%, and the ultrasonic extracts were 4%, 8%, 19%, and 42%. The anti-inflammatory effect of the extract extracted by using ultrasonic wave was higher than the anti-inflammatory effect of lipoxygenase by the hot water extraction method.

As a result, the ultrasonic extraction method used in the preparation of the natural complex extract of the present invention has the advantage that the strength of the ultrasonic energy change can be adjusted according to the raw material to be extracted. Therefore, the extraction efficiency is very high depending on the raw materials, and the possibility of denaturation and destruction of the active ingredient exhibiting pharmacological activity is minimized, and the production speed is faster than that of the conventional hydrothermal extraction method, which shortens the extraction time and thus has the characteristics of low cost and low energy use. It was confirmed.

Cosmetic composition containing a natural complex extract excellent in antioxidant and whitening effect and anti-inflammatory effect according to the present invention may have a conventional formulation, for example, softening water, facial essence (mask), nutrition lotion, nutrition cream, sun It may be formulated as block cream, body lotion, body essence and the like. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Hereinafter, a representative facial essence and a nourishing cream composition of a cosmetic composition having a skin protection effect against ultraviolet rays and a moisturizing elasticity function will be described as an example.

In addition, the cosmetic composition containing a natural complex extract excellent in antioxidant, whitening and anti-inflammatory effects according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste. .

Although the present invention has been described in connection with the above-mentioned preferred embodiments, the scope of the present invention is not limited to these embodiments, and the scope of the present invention is defined by the following claims, and equivalent scope of the present invention. It will include various modifications and variations belonging to.

Cosmetics including natural complex extract prepared by the extraction method extracted by using the ultrasonic wave of the present invention through the above examples and experimental examples relates to a cosmetic composition having a skin protection effect and moisturizing elasticity against ultraviolet rays, in more detail Is excellent in comparison with hydrothermal extraction and solvent extraction by using ultrasonic extraction method of natural complex extracts consisting of extracts consisting of situation mushrooms, ganoderma lucidum mushrooms, pine mushrooms, extracts consisting of ginseng, red ginseng, black ginseng and Gaza extract It has the effect of absorbing UV rays, has a high specific absorption, and has good solubility, affinity, and stability for various bases, additives, and organic solvents for cosmetics, and has a synergistic effect of remarkably increasing the UV protection index, especially when used in combination with existing sunscreens. It has an effect.

Claims (6)

The raw materials grouped into the first group consisting of situation mushrooms, ganoderma lucidum mushrooms and pine mushrooms, the second group consisting of fresh ginseng, red ginseng and black ginseng, and the third group consisting of Gaza are first washed with purified water and then finely grouped 1 to 10% by weight of the extraction solvent on the basis of the pulverized solids for each group, and immersed in a low temperature by immersing it in a mixture of purified water or purified water and ethanol 1:99 to 50:50 Natural complex extract obtained by ultrasonic extraction at 20 kHz to 1200 to 2400 W / L output delete delete Cosmetic composition comprising the natural complex extract of claim 1 to 2 to 10% by weight as an active ingredient delete delete

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