KR100898307B1 - Cosmetic composition with the antioxidant effect protecting skins aging - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antioxidant effect by inhibiting skin aging, and more particularly, extracts abundant and useful active ingredients from the major herbal plants composed of gold, silver, white ginseng, red ginseng, golden, gallnut, green tea, green leaves, and ginkgo biloba. It contains a natural extract (Phyto Age-L) consisting of a combination to protect the skin from a variety of sensitive stress to keep the skin soft and shiny to provide a cosmetic composition having an antioxidant effect by inhibiting skin aging.

Skin protection, cosmetics, gold and silver, earl, goose

Description

Cosmetic composition with the antioxidant effect protecting skin aging

1 is a graph showing the active oxygen scavenging activity according to the concentration of the herbal plant extract (Phyto Age-L) of the present invention,

Figure 2 is a graph showing the inhibitory activity of tyrosinase activity according to the concentration of herbal plant extract (Phyto Age-L) of the present invention,

Figure 3 is a graph showing the inhibitory activity of lipoxygenase activity according to the concentration of the herbal plant extract (Phyto Age-L) of the present invention

The present invention relates to a cosmetic composition having an antioxidant effect by inhibiting skin aging, and more specifically, skin aging by containing extracts of major herbal plants composed of gold, silver, white ginseng, red ginseng, golden, galleng, green tea, green leaves, and ginkgo biloba. It relates to a cosmetic composition that inhibits and has an antioxidant effect.

Generally, there are two kinds of skin aging phenomena, which are natural aging that starts from 25 to 26 years old and photoaging due to ultraviolet rays (or exogenous aging). Natural aging (endogenous aging) is an inevitable phenomenon that comes with age and is inevitable. It is transmitted through genes and is irresistible. Active oxygen generated during respiration is known as the main cause. Another aging phenomenon, photoaging (exogenous aging) refers to aging that occurs because the skin is always exposed to the external environment. Most of them are caused by exposure to ultraviolet rays, so if you are careful to avoid them, you can be a big difference from endogenous aging. The main cause of such photoaging is known to be due to the active oxygen generated when sunlight (ultraviolet rays). Ultraviolet rays contained in the sun's rays are divided into long, medium, and short wavelengths according to their wavelength ranges. These ultraviolet rays are known to cause various side effects on the skin.

Short wavelength ultraviolet rays in ultraviolet light are very harmful to the human body, but until now they are absorbed by the ozone layer in the atmosphere and hardly reach the earth's surface. Medium-wavelength ultraviolet rays, which cause photoallergy and more energy, cause erythema, blistering, burns, skin cancer, pigmentation, and degeneration of skin proteins. Importantly, various sunscreens have been developed to block ultraviolet rays.

Conventional ultraviolet absorbers include compounds such as benzophenone derivatives, paraamino derivatives, cinnamic acid derivatives and triazine derivatives, and inorganic pigments such as titanium dioxide and zinc oxide have been used as ultraviolet scatterers. It absorbs only the ultraviolet rays of the intestinal ultraviolet rays and is a chemical synthetic substance that is likely to cause other side effects on the skin, such as irritation and allergies. In addition, UV scattering agent is opaque when used as a cosmetic in addition to ultraviolet light as well as visible light has been pointed out that the feeling is poor when applied to the skin.

On the other hand, a substance having an ultraviolet absorbing action extracted from a natural product generally has a low irritation, and has other pharmacological actions such as anti-inflammatory, astringent, and antimicrobial effects, and thus has been promising as a ultraviolet absorber conventionally incorporated into cosmetics. Efforts have been made to search for and isolate ultraviolet absorbers contained in natural products.

The present invention is to solve the above problems, as part of the research to overcome the disadvantages of the known sunscreen and to develop a better sunscreen using a selective extraction process with water or organic solvents from various natural herbal plants As a result of considering the characteristics of the extract, while studying to develop a cosmetic composition having a skin protection effect, it contains extracts of major herbal plant components consisting of gold, silver, white ginseng, red ginseng, golden, gall, green tea, green leaves, and ginkgo biloba. It is suppressed to provide a cosmetic composition having an antioxidant effect.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition having anti-oxidant effect by inhibiting skin aging, and more specifically, skin containing extracts of major herbal plant components composed of gold, silver, white ginseng, red ginseng, gold, galleng, green tea, green leaves, and ginkgo biloba. It has the excellent effect of making skin shiny and bright by protecting it, and it is a fraction extraction mixed form obtained through multi-stage separation process with 1,3 butylene glycol, propylene glycol, ethanol or purified water. Solubility, affinity, and stability of the base, additives, organic solvents, and the like are good, and in particular, when used in combination with existing sunscreens, it provides a synergistic effect of significantly increasing the sunscreen index.

In the herbal plant composition according to the present invention, the composition of the main herbal plant raw materials consisting of gold silver, white pear, red ginseng, golden, galleng, green tea, green leaves, and ginkgo biloba is 10 to 20% by weight based on the dry weight of each, 10 to 20 white pears. 15% by weight, 10-15% by weight of red ginseng, 10-15% by weight of gold, 10-15% by weight, 5-15% by weight of green tea, 10-15% by weight of green leaves, 10-15% by weight of ginkgo leaves do.

At this time, as a raw material of the herbal plant extract of the present invention, gold silver, earl, red ginseng, gold, galleng, green tea, biloba, ginkgo biloba uses a low-temperature ultrasonic extraction method to improve the extraction efficiency of the extraction by immersion.

The low temperature here means less than 60 degrees Celsius. Thus, efforts have been made to minimize the effects of changes in the composition of plant extracts that may occur during extraction. The ultrasonic extraction method stabilizes the unstable structure of various useful components and has an advantage of effectively extracting the active ingredient of natural products by penetrating deeply into plant raw materials by various ranges of ultrasonic waves.

In the ultrasonic extraction method, the ultrasonic wave refers to a sound of high frequency that cannot be heard by the human ear.

The ultrasonic extraction method used in the preparation of the herbal plant extract of the present invention has the advantage that it is possible to control the intensity of the ultrasonic energy change. Therefore, the extraction efficiency is very high, and the possibility of denaturation and destruction of active ingredients exhibiting pharmacological activity is minimized, and the production speed is faster than that of the conventional hydrothermal extraction method, which shortens the extraction time and thus has the characteristics of low cost and low energy use. .

Extraction method of gold, silver, white ginseng, red ginseng, golden, gall, green tea, green leaves, ginkgo biloba used in the preparation of the herbal plant extract of the present invention, the raw material is first washed with water, then finely pulverized and 1 to 10% by weight Natural herbal plant extracts are divided into immersion in 1 to 50% of organic solvents such as ethanol and butylene glycol for 1 to 5 days, preferably 3 days, and using a low temperature ultrasonic extraction method for 1 to 5 hours. Maximize the extraction of active ingredients. At this time, 10 to 90 wt% of ethanol or butylene glycol organic solvent and 10 to 40 wt% of water are mixed and used as a mixed solvent, which can be extracted by ultrasonic method.

To the herbal plant extracts mixed in the above blending ratio, an organic solvent or purified water containing 1 to 5 times ethanol or butylene glycol is added and added during cosmetic preparation.

When the herbal plant extract according to the present invention is used in the cosmetic composition, the herbal plant extract prepared above is characterized in that it contains 0.1 to 5% by weight, preferably 2% by weight. At this time, when the addition of 0.1% by weight or less, the skin protection effect against ultraviolet rays of the present invention is insufficient, and when used at 5% by weight or more, it is difficult to apply the product due to the strong odor and color according to the unique flavor in the final product. This was limited to 5% by weight, more preferably 2% by weight.

Cosmetic compositions having a skin soothing effect according to the present invention may have a conventional formulation, for example, may be formulated with softening water, astringent makeup, nutrient cosmetics, nutrition cream, body lotion, body oil, body essence and the like. . The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Below, the typical skin and lotion composition of the cosmetic composition which has a skin protection effect to an ultraviolet-ray is demonstrated to an example.

In addition, the cosmetic composition having a skin protection effect against ultraviolet rays according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or taste.

Hereinafter, the cosmetic composition having the skin protection effect against ultraviolet rays of the present invention will be described in more detail with reference to the present invention, but the present invention is not limited to these examples.

Example 1 Preparation of Herbal Plant Extracts

Herbal plant extracts used dried herbal plant raw materials in a dried state.

Gold, silver, white ginseng, red ginseng, golden, peninsula, green tea, green leaves, ginkgo biloba extract method used in the preparation of the herbal plant extract of the present invention 20% by weight of gold and 100% by weight of the other raw materials, respectively It was used as a standard, washed first with water, then finely pulverized, and then crushed 1 to 10% by weight of crushed with ethanol for 3 days and the step of using a low-temperature ultrasonic extraction method for 3 hours natural herbal plant The active ingredient of the extract was maximized extraction. At this time, the raw material was extracted by the low temperature ultrasonic extraction method by mixing 70: 30% by weight of ethanol and water as a mixed solvent.

As shown in Table 1, the mixed herbal plant raw materials were extracted by low temperature ultrasonic extraction, filtered, and concentrated under reduced pressure to obtain 2 g of herbal plant extracts.

Botanical extract consisting of gold, silver, peony, red ginseng, golden, gallastula, green tea, green leaf, ginkgo biloba turn Kinds Weight before extraction (g) Weight after extraction (g) One Gold coins 2.0 2 Earl 1.2 3 Gosam 1.2 4 Gold 1.2 5 gallnut 1.1 6 green tea 1.1 7 Maple Leaf 1.1 8 Ginkgo leaves 1.1 Sum 10.0 2.0

Example 2 Preparation of Skin Lotion with Skin Protection Effect According to the Present Invention

A skin lotion was prepared at the composition ratio as shown in Table 2 below. The alcohol part was produced by dissolving in the order of MP, tween 20, NP-12 in alcohol distilled at 78 ° C in the order of grain, and the purified water part was glycerin, 1,3-BG, DC-193, hyaluronic acid in sterilized purified water. Prepared by dissolving in the order of, allantoin, citric acid, sodium citric acid and then mixed with the herbal plant extract prepared in Example 1 to prepare a skin lotion.

Classification Raw material name Weight (% by weight) Alcohol part ethanol 7 Methylparahydroxybenzoic acid 0.1 tween-20 0.2 Nonylphenylether-12 0.2 Purified water Purified water 89.01 glycerin 0.1 1,3-butylene glycol 0.2 Alanine 193 0.1 Hyaluronic acid 0.5 Allantoin 0.05 Citric acid 0.01 Citric acid 0.03 Herbal plant parts Herbal plant extracts 2.5 Sum 100

Example 3 Preparation of Milk Lotion with Skin Protection Effect According to the Present Invention

Milk lotion was prepared in the same manner as in Example 2.

Classification Raw material name Weight (% by weight) Alcohol part Stearic acid 2.1 Cetanol 1.3 Liquid paraffin 7.0 Squalene 0.5 Isopropyl myristate 1.0 Glyceryl Monostearate 0.5 Arasel-60 0.85 tween-20 0.1 Isostearyl-isostearate 0.5 Propylparaben 0.1 Alanine 200F / 100CS 0.1 Purified water Purified water 71.65 Triethanolamine 0.7 Propylene glycol 7.0 glycerin 3.0 Methylparahydroxybenzoic acid 0.1 Herbal plant Herbal plant extracts 3.5 Sum 100

Experimental Example 1 Check the skin irritation and protective effect of the skin lotion according to the present invention

Apply to the forearm of 40 healthy men and women (20 men, 20 women) by applying a predetermined amount (10m / 5cm2) of the skin lotion prepared in Example 2, and shielding the other parts with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, skin side effects such as erythema do not occur on the skin, and thus the skin lotion has no phototoxicity and has a skin protection effect.

Experimental Example 2: Confirmation of skin irritation and protective effect of milk lotion according to the present invention

Apply a lotion of milk lotion prepared in Example 3 (10m / 5cm2) to the forearm of 40 healthy men and women (20 males, 20 females) and shield the rest with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, the side effects such as erythema do not occur on the skin, and thus the milk lotion has no phototoxicity and has a skin protection effect.

Experimental Example 3: Skin stability confirmation test of skin lotion and milk lotion according to the present invention

The skin patch and milk lotion prepared in Examples 2 and 3 were subjected to a human patch test on the forearm of 40 healthy adult men and women (20 males and 20 females). Wipe the forearm of the subject well with 70% ethanol, and then place a pin chamber containing 20 μl of the skin lotion and milk lotion prepared in Examples 2 and 3 on a conventional cosmetic base using adhesive tape for 24 hours. Closed attachment. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. Experimental results As shown in Table 4, in the human patch test to confirm the skin irritation of the skin lotion and milk lotion according to the present invention did not show a positive response was confirmed that there is no irritation to the human skin.

sample After 24 hours (n = 40) After 48 hours (n = 40) Remarks Skin lotion 0/40 0/40 Number of reaction quantums / total number of targets Milk lotion 0/40 0/40

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Experimental Example 5 Antioxidant Effect by Active Oxygen Scavenging Ability

Skin aging processes with free radicals include lipid peroxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal crosslinks. Most of these reactions are radical reactions that involve an automatic oxidation process. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the reducing power as an electron-giving ability, the stronger the antioxidant. A reagent that measures the reducing power of antioxidants is 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. Maximum absorption at 517 nm is shown and when reduced, absorption is lost at 517 nm. The magnitude of the reducing power of the herbal plant extract of the present invention is expressed by the radical scavenging activity (SCcavenging activity, SC50) and SC50 represents the concentration required to reduce the concentration of DPPH by 50%. For the herbal plant extract of the present invention is shown in Figure 1 the results shown by measuring the SC50 by concentration. That is, in Figure 1, the ultrasonic extraction method was the best active oxygen scavenging rate than the general hydrothermal extraction method.

Experimental Example 6: Determination of Tyrosinase Activity

When tyrosinase is activated by UV or ambient environment, tyrosine (DOPA) is produced as dopachrome, an intermediate of melanin, and is converted into melanin polymer by an automatic oxidation reaction. Through the effect of inhibiting the activity of tyrosinase enzymes can be expected to whitening effect that prevents melanin production to make the skin clear and bright. Inhibition of tyrosinase activity through the inhibition of dopachrome production can be measured by melanin pigmentation and melancholy inhibition degree and indirectly whitening efficacy. According to the herbal plant extract of the present invention according to the concentration to examine the degree of inhibiting tyrosinase activity prepared by the normal hot water extraction method and ultrasonic extraction method, the effect by the ultrasonic extraction method was excellent as shown in FIG.

Experimental Example 7: anti-inflammatory effect according to the inhibitory ability of lipoxygenase

Lipoxygenase (5-LO, 5-lipoxygenase) adds molecular oxygen to unsaturated fatty acids with 1,4-cis, cis-pentadiene (1,4-pentadiene) structures in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. It is an oxidase that produces 1,3-cis, trans-diene 5-hydroperoxide. It is the enzyme involved in the first reaction of leucotriene biosynthesis from arachidonic acid, which is involved in various inflammatory and allergic diseases. Therefore, anti-inflammatory effect can be evaluated by measuring inhibitory activity of lipoxygenase using SLO (soybean lipoxygenase) similar to 5-LO structurally and mechanism.

Figure 3 is a graph showing the anti-inflammatory effect according to the inhibitory ability of lipoxygenase according to the concentration of the herbal plant extract prepared by the present invention, when it is described in more detail, the ultrasonic extraction method has a lipoxygenase inhibitory activity than the general hydrothermal extraction method It was found to be excellent.

Cosmetics containing the herbal plant extract of the present invention through the above Examples and Experimental Examples by maximizing the effective extract of the main herbal plant components consisting of gold, silver, white ginseng, red ginseng, golden, gall, green tea, biloba, ginkgo biloba It is to provide an excellent cosmetic composition.

Claims (4)

A cosmetic composition containing herbal plant extracts that inhibits skin aging and has an antioxidant effect, Cosmetic composition having antioxidant effect by inhibiting skin aging, characterized by containing herbal plant extract extracted by low-temperature ultrasonic extraction method consisting of gold, silver, pear, red ginseng, golden, five gall, green tea, green leaf, ginkgo biloba The method of claim 1, The composition of the herbal plant extract is based on the dry weight of each 10 ~ 20% by weight, silver 10 ~ 15% by weight, ginseng 10 ~ 15% by weight, golden 10 ~ 15% by weight, 5 to 10% by weight 5 times, Cosmetic composition having an antioxidant effect by inhibiting skin aging, characterized in that the mixture of green tea 10 to 15% by weight, green leaf 10 to 15% by weight, ginkgo leaves 10 to 15% by weight The method of claim 1, A cosmetic composition having an antioxidant effect by inhibiting skin aging, further comprising a medium wavelength ultraviolet absorber The method of claim 3, wherein Medium wavelength ultraviolet absorber cosmetic composition having an antioxidant effect by inhibiting skin aging, characterized in that at least one selected from benzophenone derivatives, paraamino benzoic acid derivatives, cinnamic acid derivatives, salicylic acid derivatives, triazine derivatives

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