CN114469795A - Preparation method of SOD cosmetic additive extract - Google Patents
Preparation method of SOD cosmetic additive extract Download PDFInfo
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- CN114469795A CN114469795A CN202011513061.XA CN202011513061A CN114469795A CN 114469795 A CN114469795 A CN 114469795A CN 202011513061 A CN202011513061 A CN 202011513061A CN 114469795 A CN114469795 A CN 114469795A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention discloses a preparation method of an SOD cosmetic additive extract, which comprises the following steps: (1) pulverizing a certain amount of radix Paeoniae alba and flos Lonicerae into powder, packaging the powder with gauze bag, steaming in special steam box for a certain time, cooling, squeezing gauze bag, squeezing water, pouring out the powder in gauze bag, and oven drying to obtain dry powder; (2) adding the dry powder into phosphate buffer solution containing hexafluoroisopropanol and ionic liquid, cellulase and pectinase, heating, soaking, extracting, filtering to obtain filtrate, standing for layering, collecting ionic liquid layer, and finally obtaining SOD cosmetic additive extract containing radix Paeoniae alba and flos Lonicerae. The powder adopted by the invention is subjected to special sterilization treatment of the steam box, and the use safety is high.
Description
Technical Field
The invention relates to the technical field of SOD cosmetics, in particular to a preparation method of an SOD cosmetic additive extract.
Background
Superoxide dismutase (SOD) is an antioxidant metalloenzyme existing in organisms, can catalyze superoxide anion free radical disproportionation to generate oxygen and hydrogen peroxide, plays a vital role in body oxidation and antioxidant balance, has special effects on preventing cancer cell diffusion and transfer, resisting radiation, enhancing immunity, resisting aging and beautifying, and is inseparable from occurrence and development of many diseases. Because of the above effects of SOD, it has wide market prospect in the fields of medicine, food and cosmetic health products, and food, beverage, cosmetic and health products containing little SOD are on the market at home and abroad. At present, SOD is mostly obtained from animal blood and pancreas at home and abroad. Although many plants also contain SOD, the preparation of SOD from plant materials is generally carried out in proper buffer solution, most of the extractions are carried out by using the buffer solution as a base solution, the yield is low, the activity of the extractives is not high, and the powder adopted in the preparation is not sterilized by a special process, so that certain problems exist in the aspect of use safety.
Disclosure of Invention
The invention aims to solve the technical problem of providing a preparation method of an SOD cosmetic additive extract aiming at the defects in the prior art.
According to the invention, the preparation method of the SOD cosmetic additive extract comprises the following steps: (1) pulverizing a certain amount of radix Paeoniae alba and flos Lonicerae into powder, packaging the powder with gauze bag, steaming in special steam box for a certain time, cooling, squeezing gauze bag, squeezing water, pouring out the powder in gauze bag, and oven drying to obtain dry powder; (2) adding the dry powder into phosphate buffer solution containing hexafluoroisopropanol and ionic liquid, cellulase and pectinase, heating, soaking, extracting, filtering to obtain filtrate, standing for layering, collecting ionic liquid layer, and finally obtaining SOD cosmetic additive extract containing radix Paeoniae alba and flos Lonicerae.
In the above method for preparing the SOD cosmetic additive extract, the step (1) of steaming in a special steaming box for a certain time comprises: steaming at 110-150 deg.C for 5-15 min.
In the preparation method of the SOD cosmetic additive extract, a plurality of steaming plates are arranged on a box body of the special steaming box from top to bottom, two ends of the bottom of each steaming plate are provided with slideways, and the white paeony root and the honeysuckle in the step (1) are crushed into powder, packaged by a gauze bag and placed on the steaming plates.
In the preparation method of the SOD cosmetic additive extract, the bottom of the special steam box is provided with a steam generator, and steam generated by the steam generator is used for steaming the white paeony root and honeysuckle powder gauze bags on the steam plate at high temperature.
In the preparation method of the SOD cosmetic additive extract, the top of the box body of the special steam box is provided with a plurality of steam outlets.
In the above method for preparing the SOD cosmetic additive extract, the step (1) of squeezing water off, pouring out the powder in the gauze bag, and drying into dry powder comprises: squeezing water, pouring out the powder in the gauze bag, placing on a steaming plate, and only opening a drying heating pipe in a special steaming box body to dry the powder.
In the above method for preparing the SOD cosmetic additive extract, the step (1) of taking a certain amount of white peony root and honeysuckle flower comprises: according to the weight percentage of white paeony root: and (5) honeysuckle flower: 2-3 taking radix paeoniae alba and honeysuckle.
In the preparation method of the SOD cosmetic additive extract, the white paeony root and the honeysuckle flower are crushed and uniformly mixed in the step (1) to form powder of 40-60 meshes.
In the above method for preparing the SOD cosmetic additive extract, the heating, soaking, extracting and filtering in the step (2) to obtain a filtrate comprises: heating to 40-50 deg.C, soaking and extracting for 2-4 hr, and filtering to obtain filtrate.
In the preparation method of the SOD cosmetic additive extract, the mass ratio of the cellulase to the pectinase in the step (1) is 2: 1-2.
The white paeony root and the honeysuckle are crushed into powder, then the powder is packaged by a gauze bag, the gauze bag is put into a special steam box for steaming for a certain time, the gauze bag is extruded after the gauze bag is cooled, the moisture is extruded, the powder in the gauze bag is poured out and dried into dry powder, and the powder is subjected to special sterilization treatment of the steam box, so that the use safety is high.
Drawings
A more complete understanding of the present invention, and the attendant advantages and features thereof, will be more readily understood by reference to the following detailed description when considered in conjunction with the accompanying drawings wherein:
FIG. 1 is a flow chart of the preparation method of SOD cosmetic additive extract;
FIG. 2 is a schematic structural view of the steam box;
FIG. 3 is a schematic structural view of the case of FIG. 2;
FIG. 4 is a schematic view of the door of FIG. 2;
FIG. 5 is a schematic structural view of the lock seat in FIG. 4;
FIG. 6 is a cross-sectional view of FIG. 5;
FIG. 7 is a partial cross-sectional view of FIG. 5;
FIG. 8 is a schematic front internal view of FIG. 5;
FIG. 9 is a schematic view of the back side internal structure of FIG. 5
FIG. 10 is a schematic view of the push block of FIG. 9
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.
The phosphate buffer solution used in the method for preparing the SOD cosmetic additive extract of the present embodiment may be a buffer solution with a pH of 4.5-5.5 prepared from two or more of sodium/potassium dihydrogen phosphate, disodium/potassium hydrogen phosphate, sodium hydroxide, and phosphoric acid. The technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other. Specifically, as shown in fig. 1, the method comprises the following steps: (1) pulverizing a certain amount of radix Paeoniae alba and flos Lonicerae into powder, packaging the powder with gauze bag, steaming in special steam box for a certain time, cooling, squeezing gauze bag, squeezing water, pouring out the powder in gauze bag, and oven drying to obtain dry powder; (2) adding the dry powder into phosphate buffer solution containing hexafluoroisopropanol and ionic liquid, cellulase and pectinase, heating, soaking, extracting, filtering to obtain filtrate, standing for layering, collecting ionic liquid layer, and finally obtaining SOD cosmetic additive extract containing radix Paeoniae alba and flos Lonicerae.
Example 1
The preparation method comprises the following steps:
(1) 500g of white paeony root and 250g of honeysuckle flower are crushed and uniformly mixed, the mixture is sieved by a 50-mesh sieve, the powder is packed by a gauze bag, the gauze bag is placed in a special steam box to be steamed for 5min at the high temperature of 110 ℃, the gauze bag is extruded after being cooled, the water is extruded, the powder in the gauze bag is poured out and dried into dry powder, phosphate buffer solution (pH5.0, 4.0kg, wherein 125g of hexafluoroisopropanol and 500g of ionic liquid 1-butyl-3-methylimidazole acetate, cellulase (4g, 30u/mg) and pectinase (3g, 30u/mg) are added, the mixture is heated to 45 ℃, soaked and extracted for 3 hours, the filtrate is obtained by filtering, and the ionic liquid layer is collected after standing and layering;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with ethanol and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 15.52g of the extracts of the white paeony root and the honeysuckle flower.
Example 2
The preparation method comprises the following steps:
(1) 500g of white paeony root and 200g of honeysuckle flower are crushed and uniformly mixed, the mixture is sieved by a 40-mesh sieve, powder materials are packed by a gauze bag, the gauze bag is placed in a special steam box to be steamed for 10min at a high temperature of 130 ℃, the gauze bag is extruded after being cooled, water is extruded out, the powder materials in the gauze bag are poured out and dried into dry powder, phosphate buffer solution (pH5.0, 3.5kg, wherein 105g of hexafluoroisopropanol and 525g of ionic liquid 1-butyl-3-methylimidazole acetate, cellulase (5g, 20u/mg) and pectinase (2g, 40u/mg) are added, the mixture is heated to 50 ℃, soaked and extracted for 2 hours, filtrate is obtained after filtration, and an ionic liquid layer is collected after standing and layering;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) for 3 times by using ethyl acetate with the same volume, combining ethyl acetate phases, sequentially washing by using ethanol and saturated sodium chloride solution, drying the organic layer by using anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 14.68g of the extracts of the white paeony root and the honeysuckle flower.
Example 3
The preparation method comprises the following steps:
(1) 500g of white paeony root and 300g of honeysuckle flower are crushed, mixed uniformly, sieved by a 60-mesh sieve, packed by a gauze bag, put into a special steam box to be steamed for 15min at the high temperature of 150 ℃, the gauze bag is extruded after being cooled, the moisture is extruded, the powder in the gauze bag is poured out and dried into dry powder, phosphate buffer solution (pH4.5, 4.5kg, wherein the phosphate buffer solution contains 150g of hexafluoroisopropanol and 450g of ionic liquid 1-butyl-3-methylimidazole acetate, cellulase (2.5g, 40u/mg) and pectinase (2.5g, 20u/mg), heated to 40 ℃, soaked and extracted for 4 hours, filtered to obtain filtrate, and the ionic liquid layer is collected after standing and layering;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) for 3 times by using ethyl acetate with the same volume, combining ethyl acetate phases, sequentially washing by using ethanol and saturated sodium chloride solution, drying the organic layer by using anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 16.89g of the extracts of the white paeony root and the honeysuckle flower.
Comparative example 1
The preparation method comprises the following steps:
(1) pulverizing radix Paeoniae alba 400g and flos Lonicerae 100g, mixing, sieving with 80 mesh sieve, and adding ionic liquid [ BMIM ]]PF6Phosphate buffer solution (pH5.0, 5.0kg, containing 1.0kg of [ BMIM ]]PF6) Heating to 40 deg.C, soaking and extracting for 5 hr, filtering, removing residue, standing for layering, and collecting ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with water and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain 7.11g of the extracts of the white paeony root and the honeysuckle flower.
Comparative example 2
The preparation method comprises the following steps:
(1) crushing and uniformly mixing 500g of white paeony root and 125g of sophora flower, sieving with a 50-mesh sieve, adding a phosphate buffer solution (pH5.0, 4.0kg, wherein 125g of hexafluoroisopropanol and 500g of ionic liquid 1-butyl-3-methylimidazolium acetate, cellulase (4g, 30u/mg) and pectinase (3g, 30u/mg) into the mixture, heating the mixture to 45 ℃, soaking and extracting the mixture for 3 hours, filtering to obtain a filtrate, standing and layering, and collecting an ionic liquid layer;
(2) and (2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with ethanol and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 9.45g of the extracts of the white paeony root and the sophora flower.
Comparative example 3
The preparation method comprises the following steps:
(1) 500g of white paeony root and 250g of honeysuckle are crushed and mixed uniformly, the mixture is sieved by a 50-mesh sieve, phosphate buffer solution (pH5.0, 4.0kg, wherein 125g of hexafluoroisopropanol and 500g of ionic liquid 1-ethylamino-3-methylimidazole hydroxide, cellulase (4g, 30u/mg) and pectinase (3g, 30u/mg) are added, the mixture is heated to 45 ℃ for soaking and extraction for 3 hours, filtrate is obtained by filtration, and an ionic liquid layer is collected after standing and layering;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with ethanol and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 13.21g of the extracts of the white paeony root and the honeysuckle flower.
Comparative example 4
The preparation method comprises the following steps:
(1) crushing and uniformly mixing 500g of white paeony root and 250g of honeysuckle, sieving with a 50-mesh sieve, adding a phosphate buffer solution (pH5.0, 4.0kg, wherein the phosphate buffer solution contains 500g of ionic liquid 1-butyl-3-methylimidazolyl acetate, cellulase (4g, 30u/mg) and pectinase (3g, 30u/mg), heating to 45 ℃, soaking and extracting for 3 hours, filtering to obtain a filtrate, standing for layering, and collecting an ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with ethanol and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating and drying the filtrate to obtain 12.86g of the extracts of the white paeony root and the honeysuckle.
Comparative example 5
The preparation method comprises the following steps:
(1) crushing 500g of white paeony root, uniformly mixing, sieving by a 50-mesh sieve, adding phosphate buffer solution (pH5.0, 4.0kg, wherein 125g of hexafluoroisopropanol, 500g of ionic liquid 1-butyl-3-methylimidazolium acetate, cellulose (4g, 30u/mg) and pectinase (3g, 30u/mg) into phosphate buffer solution (containing 125g of hexafluoroisopropanol, 500g of ionic liquid 1-butyl-3-methylimidazolium acetate), heating to 45 ℃, soaking and extracting for 3 hours, filtering to obtain filtrate, standing for layering, and collecting an ionic liquid layer;
(2) extracting the ionic liquid layer obtained in the step (1) with ethyl acetate of the same volume for 2 times, combining ethyl acetate phases, sequentially washing with ethanol and saturated sodium chloride solution, drying the organic layer with anhydrous sodium sulfate, filtering, concentrating the filtrate, and drying to obtain 8.89g of the white paeony root extract;
(3) repeating the steps (1) and (2), wherein the extraction target is 250g of honeysuckle to obtain 4.36g of honeysuckle extract;
(4) mixing the above obtained radix Paeoniae alba extract and flos Lonicerae extract to obtain radix Paeoniae alba and flos Lonicerae extract 13.25 g.
Effects of the embodiment
The final extracts obtained in examples 1-3 and comparative examples 1-5 were respectively designated as A, B, C, D, E, F, G, H, and extracts A-H were dissolved in DMSO to prepare solutions having a mass concentration of 1.0mg/mL, and the inhibition rate of tyrosinase, DPPH radical scavenging ability, superoxide anion scavenging ability, and SOD enzyme activity were measured according to the method in background CN109091440, and the results are shown in the following tables.
From the detection results in the table, the extract obtained by simultaneously extracting the white paeony root and the honeysuckle by adopting the extraction method disclosed by the invention has excellent tyrosinase inhibition rate, DPPH free radical scavenging capacity, superoxide anion scavenging capacity and SOD enzyme activity. Therefore, the plant extract of the invention takes the white paeony root and the honeysuckle as the active substance sources, and the process is simple through the combined action of the cellulase, the pectinase and the mixed extracting solution of the invention, so that the active ingredients in the plant are fully extracted, the activity of the active ingredients is kept, the basic function and the property of the original active substances are not changed, the extraction rate is high, and the obtained extract has better DPPH free radical scavenging capacity, superoxide anion scavenging capacity, tyrosinase inhibition rate and SOD enzyme activity and can be used as an SOD cosmetic additive.
The special oven in this embodiment includes box 1, chamber door 2 and steam generator 3 as shown in fig. 2 to 10, chamber door 2 is articulated and sealed cooperation with box 1 front portion, and steam generator 3 is located the bottom of box 1, and box 1 top-down is equipped with a plurality of and evaporates board 4, evaporates the bottom both ends of board 4 and all installs slide 5, evaporates and can set up the mesh on the board 4 so that advance steam, and box 1 top is equipped with a plurality of steam outlet 6, and steam outlet 6 is used for discharging steam.
The right side of the box door 2 is hinged with the right side of the front part of the box body 1, the left side of the front part of the box body 1 is provided with a lock cylinder 7, the lock cylinder 7 is vertically arranged and is vertically communicated, the box door 2 is provided with a lock seat 8, an upper lock rod 9, a lower lock rod 10, an upper abutting spring 11, a lower abutting spring 12 and a push block 13 are arranged in the lock seat 8, the bottom of the upper lock rod 9 and the top of the lower lock rod 10 are respectively provided with an upper lock pin 14 and a lower lock pin 15, the lock seat 8 is externally provided with a pull handle 16 in transmission connection with the push block 13, the push block 13 overcomes the upper abutting spring 11 and the lower abutting spring 12 under the action of the pull handle 16 to push the upper lock rod 9 upwards and push the lower lock rod 10 downwards, so that the upper lock pin 14 and the lower lock pin 15 are respectively separated from the upper end and the lower end of the lock cylinder 7, the upper lock rod 9 and the lower lock rod 10 can be separated from the upper end and the lower end of the lock cylinder 7 by pulling the pull handle 16 to realize unlocking, the unlocking is extremely simple in operation, so that the door 2 can be opened conveniently.
The lock base 8 of the embodiment is provided with a first cavity 17 and a second cavity 18, the first cavity 17 is provided with an upper cavity 19 and a lower cavity 20, the upper lock rod 9 and the lower lock rod 10 are respectively positioned in the upper cavity 19 and the lower cavity 20, the upper abutting spring 11 is connected between the top wall of the upper cavity 19 and the top of the upper lock rod 9, the lower abutting spring 12 is connected between the bottom wall of the lower cavity 20 and the bottom of the lower lock rod 10, the upper lock pin 14 of the upper lock rod 9 is inserted into the upper end of the lock cylinder 7 under the action of the upper abutting spring 11, the lower lock pin 15 of the lower lock rod 10 is inserted into the lower end of the lock cylinder 7 under the action of the lower abutting spring 12, the push block 13 is positioned in the second cavity 18, the top and the bottom of the push block 13 are respectively provided with an upper inclined surface 21 and a lower inclined surface 22, the upper inclined surface 21 and the lower inclined surface 22 are respectively abutted against the upper lock rod 9 and the lower lock rod 10, so that when the handle 16 drives the push block 13 to move leftwards, the push block 13 overcomes the action of the upper abutting spring 11 through the upper abutting spring 21 to drive the upper lock rod 9 to move upwards, the push block 13 overcomes the action of the lower pressing spring 12 through the lower inclined surface 22 to drive the lower lock rod 10 to move downwards, so that the upper lock pin 14 and the lower lock pin 15 are separated from the upper end and the lower end of the lock cylinder 7 respectively to unlock the box door 2. Specifically, the right ends of the upper lock rod 9 and the lower lock rod 10 are respectively connected with a downward upper abutting rod 23 and an upward lower abutting rod 24, the bottom of the upper abutting rod 23 and the top of the lower abutting rod 24 are respectively provided with an abutting inclined surface, the bottom of the upper abutting rod 23 and the top of the lower abutting rod 24 are respectively abutted against the upper inclined surface 21 and the lower inclined surface 22, the push block 13 pushes the upper abutting rod 23 through the action of the upper inclined surface 21 and the abutting inclined surface, and the push block 13 pushes the lower abutting rod 24 through the action of the lower inclined surface 22 and the abutting inclined surface.
A horizontal locking plate 25 and a vertical locking plate 26 are connected to the left end of the pushing block 12, the horizontal locking plate 25 is located between the pushing block 12 and the vertical locking plate 26, an upper locking column 27 and a lower locking column 28 are arranged at the right end of the horizontal locking plate 25, an upper locking hole 29 and a lower locking hole 30 are respectively arranged at the left ends of the upper pressing rod 23 and the lower pressing rod 24, an upper fixing plate 31 and a lower fixing plate 30 are arranged in the middle of the first cavity 17, after the upper locking pin 14 and the lower locking pin 15 are respectively inserted into the upper end and the lower end of the lock cylinder 7, the upper locking column 14 and the lower locking column 15 respectively partially extend into the upper locking hole 29 and the lower locking hole 30, an intermediate fixing plate 31 is arranged in the middle of the first cavity 17, the upper part and the lower part of the intermediate fixing plate 31 are respectively located in the upper cavity 19 and the lower cavity 20, a damping spring 32 is arranged between the intermediate fixing plate 31 and the vertical locking plate 26, after the upper locking pin 14 and the lower locking pin 15 are respectively inserted into the upper end and the lower end of the lock cylinder 7, the damping spring 32 is in a compressed state, and the upper locking column 14 and the lower locking column 15 respectively partially extend into the upper locking hole 29 and the lower locking hole 30, this maintains the locked state and prevents a small force from pushing the push block 13 to the left to unlock easily. Meanwhile, after the upper lock pin 14 and the lower lock pin 15 are respectively inserted into the upper end and the lower end of the lock cylinder 7, the bottom of the upper pressing rod 23 and the top of the lower pressing rod 24 are respectively attached to the top and the bottom of the horizontal lock plate 25, that is, the bottom of the upper pressing rod 23 and the top of the lower pressing rod 24 are respectively and approximately located at the middle positions of the top and the bottom of the horizontal lock plate 25, so that the pushing block 13 is pushed to move leftwards, the upper lock column 14 and the lower lock column 15 can be respectively separated from the upper lock hole 29 of the upper pressing rod 23 and the lower lock hole 30 of the lower pressing rod 24, and then the upper pressing rod 23 is driven to move upwards and the lower pressing rod 24 is driven to move downwards, so that unlocking is smoothly realized.
An upper fixing plate 33 is arranged between the upper cavity 19 and the second cavity 18, a lower fixing plate 34 is arranged between the lower cavity 19 and the second cavity 18, a downward upper limiting plate 35 and an upward lower limiting plate 36 are respectively arranged at the bottom of the upper lock rod 9 and the top of the lower lock rod 10, the upper limiting plate 35 and the lower limiting plate 36 are respectively attached to the middle fixing plate 31, the upper abutting rod 23 and the lower abutting rod 24 are respectively attached to the upper limiting plate 35 and the lower limiting plate 36, and the middle fixing plate 31, the upper limiting plate 35 and the lower limiting plate 36 play a role in limiting the movement track of the upper lock rod 9 and the lower lock rod 10. A screw rod 37 is rotatably connected in the second cavity 18, an upper thread section and a lower thread section of the screw rod 37 are respectively in threaded connection with an upper thread sleeve 38 and a lower thread sleeve 39, the threads of the upper thread section and the lower thread section are opposite, an upper connecting rod 40 is connected between the upper thread sleeve 38 and the right end of the push block 13, a lower connecting rod 41 is connected between the lower thread sleeve 39 and the right end of the push block 13, a gear 42 is fixed at the middle position of the screw rod 37, the gear 42 is in meshed connection with a rack 43, the rack 43 is provided with a handle seat 44, the lock seat 8 is provided with a strip-shaped opening 45, and the handle seat 44 is positioned in the strip-shaped opening 45 and is rotatably connected with the handle 16.
When the door 2 is locked, the handle 16 and the handle seat 44 are located at the leftmost end of the strip-shaped opening 45, after the upper lock pin 14 of the upper lock rod 9 and the lower lock pin 15 of the lower lock rod 10 are respectively inserted into the upper end and the lower end of the lock cylinder 7, the bottom of the upper pressing rod 23 and the top of the lower pressing rod 24 are respectively attached to the top and the bottom of the horizontal lock plate 25, the damping spring 32 is in a compressed state, and the upper lock column 14 and the lower lock column 15 respectively partially extend into the upper lock hole 29 and the lower lock hole 30. When unlocking is required, the handle 16 is pulled rightwards, the handle 16 drives the screw rod 37 to rotate through the rack 43 and the gear 42, the upper threaded sleeve 38 moves downwards, the lower threaded sleeve 39 moves upwards, the push block 13 is pushed leftwards by the upper connecting rod 40 and the lower connecting rod 41, so that the upper lock cylinder 14 and the lower lock cylinder 15 are gradually separated from the upper lock hole 29 and the lower lock hole 30 respectively, after the upper lock cylinder 14 and the lower lock cylinder 15 are completely separated from the upper lock hole 29 and the lower lock hole 30 respectively, the bottom of the upper pressing rod 23 and the top of the lower pressing rod 24 are respectively contacted with the upper inclined surface 21 and the lower inclined surface 22 of the push block 13, and under the action of the upper inclined surface 21 and the lower inclined surface 22, the upper pressing rod 23 is communicated with the upper lock rod 9 to move upwards, the lower pressing rod 24 is communicated with the lower lock rod 10 to move downwards, so that the upper lock pin 14 of the upper lock rod 9 and the lower lock pin 15 of the lower lock rod 10 are separated from the upper end and the lower end of the lock cylinder 7, and unlocking is realized, after the handle 16 is loosened, and the damping spring 32, Each part can be reset under the action of the upper pressing spring 11 and the lower pressing spring 12.
It will be understood by those skilled in the art that the foregoing is only exemplary of the present invention, and is not intended to limit the invention to the particular forms disclosed, since various modifications, substitutions and improvements within the spirit and scope of the invention are possible and within the scope of the appended claims.
Claims (10)
1. The preparation method of the SOD cosmetic additive extract is characterized by comprising the following steps: (1) pulverizing a certain amount of radix Paeoniae alba and flos Lonicerae into powder, packaging the powder with gauze bag, steaming in special steam box for a certain time, cooling, squeezing gauze bag, squeezing water, pouring out the powder in gauze bag, and oven drying to obtain dry powder; (2) adding the dry powder into phosphate buffer solution containing hexafluoroisopropanol and ionic liquid, cellulase and pectinase, heating, soaking, extracting, filtering to obtain filtrate, standing for layering, collecting ionic liquid layer, and finally obtaining SOD cosmetic additive extract containing radix Paeoniae alba and flos Lonicerae.
2. The method for preparing the SOD cosmetic additive extract as claimed in claim 1, wherein the step (1) of steaming in a special steamer for a certain time comprises: steaming at 110-150 deg.C for 5-15 min.
3. The method for preparing the SOD cosmetic additive extract as claimed in claim 2, wherein the steaming plates are disposed on the box of the special steaming box from top to bottom, the slides are disposed at both ends of the bottom of the steaming plates, and the radix Paeoniae alba and flos Lonicerae obtained in step (1) are pulverized into powder, packaged with gauze bag, and placed on the steaming plates.
4. The method for preparing the SOD cosmetic additive extract as claimed in claim 3, wherein a steam generator is disposed at the bottom of the special steaming box, and the steam generated by the steam generator is used for steaming the gauze bags containing radix Paeoniae alba powder and flos Lonicerae powder on the steaming plate at high temperature.
5. The method for preparing the SOD cosmetic additive extract as claimed in claim 3, wherein the top of the box body of the special steaming box is provided with a plurality of steam outlets.
6. The method for preparing the SOD cosmetic additive extract as claimed in claim 3, wherein the step (1) of squeezing water out, pouring out the powder in the gauze bag, and drying the powder to dry powder comprises: squeezing water, pouring out the powder in the gauze bag, placing on a steaming plate, and only opening a drying heating pipe in a special steaming box body to dry the powder.
7. The method for preparing the SOD cosmetic additive extract as claimed in claim 1, wherein the step (1) of taking a certain amount of the white peony root and the honeysuckle flower comprises: according to the weight percentage of white paeony root: and (5) honeysuckle flower: 2-3 taking radix paeoniae alba and honeysuckle.
8. The method for preparing the SOD cosmetic additive extract according to claim 1, wherein the white peony root and the honeysuckle flower are pulverized and mixed uniformly in the step (1) to form powder of 40-60 meshes.
9. The method for preparing the SOD cosmetic additive extract as claimed in claim 1, wherein the heating soaking extraction and filtering of the filtrate in the step (2) comprises: heating to 40-50 deg.C, soaking and extracting for 2-4 hr, and filtering to obtain filtrate.
10. The method for preparing the SOD cosmetic additive extract according to claim 1, wherein the mass ratio of the cellulase to the pectinase in the step (1) is 2: 1-2.
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