JP5983908B2 - Anti-inflammatory agents and skin preparations - Google Patents

Description

TECHNICAL FIELD The present invention relates to an anti-inflammatory agent characterized by containing an aqueous component of defatted cocoons of camellia (Camellia japonica) seeds belonging to the camellia family as an active ingredient, and a method for producing an external skin product using the same. The anti-inflammatory agent of the present invention means a TGF-β1 enhancer for suppressing skin inflammation caused by ultraviolet irradiation.

With recent changes in the global environment (warming, destruction of the ozone layer, etc.), the amount of ultraviolet rays received by organisms on the earth is currently increasing, although it is generally within an acceptable range. There is growing interest in health effects as well as conventional skin beauty. That is, ultraviolet rays induce and promote aging of skin tissues such as stains, freckles, and black skin pigmentation, wrinkles, sagging, and the like, and there is a concern that skin cancer frequently occurs particularly in the western region.

The following is known about the effect of ultraviolet rays on the skin. Among ultraviolet rays contained in sunlight, long-wavelength ultraviolet rays (400 nm to 320 nm, UV-A) are involved in cellular metabolism of the cells to activate cell functions and cause skin tanning (browning). In addition, it acts on the dermis layer to denature proteins and collagen and elastin to denature the skin, thereby causing skin wrinkles and sagging to promote skin aging. In some cases, phototoxicity or photoallergic reaction is induced, and the melanin pigment produced by UV-B is oxidized to turn brown. Medium wavelength ultraviolet light (320 nm to 280 nm, UV-B) acts on the epidermis layer, but pigment cells generate melanin to protect living tissues. It is considered that the skin is tanned (sunburn) red, causing inflammation, erythema and blisters, and contributing to the formation of spots, freckles and the like. In addition, it is said that a large amount of exposure induces immune decline, skin cancer, and cataract. Short wavelength ultraviolet rays (less than 280 nm, UV-C) have a strong bactericidal action and are most destructive to living organisms, but ultraviolet rays below 290 nm are absorbed by atmospheric ozone, oxygen and nitrogen in the atmosphere, so Will not reach.

In order to prevent, ameliorate, or treat skin damage caused by ultraviolet rays, particularly UV-A and UV-B, conventional skin external preparations include cinnamic acid compounds, aminobenzoic acid derivatives, benzophenone derivatives, salicylic acid compounds, urocanic acid derivatives. UV absorbers such as zinc oxide and titanium oxide have been used. However, for example, some cinnamic acid derivatives exhibit phototoxicity, and aminobenzoic acids may be discolored or denatured into a resinous substance, for example, because of safety, stability, usability, cost, etc. A sufficient effect was not obtained. In addition, steroidal drugs such as betamethasone valerate and triamcinolone acetonide and nonsteroidal drugs such as ibuprofen piconol and bendazac have been used for the purpose of suppressing skin inflammation. However, some of them show side effects such as redness, and there are many restrictive conditions such as the need to use them appropriately under the guidance of a doctor, and they are not satisfactory.

On the other hand, the following is known about camellia. That is, camellia has a history of being used as an ornamental horticultural plant since ancient times, and fats and oils collected from seeds are used as fuel oil, hair styling, high-grade edible oil, etc., and xylem is ashed to brew sake. Seed defatted lees have been used as fertilizers for agricultural crops. There is a report that oil collected from camellia seeds is rich in oleic acid as a constituent fatty acid and reduces allergic symptoms such as childhood atopy (Non-patent Document 1). In addition, defatted cocoons contain saponins and tannins, which are processed to become insecticides (Patent Document 1), agricultural and horticultural nematode control agents (Patent Document 2), emulsifiers (Patent Document 3), etc. There is also. However, there is no example in which the components contained in the defatted cocoons of seeds are used for suppressing inflammation of living tissues.

Japanese Patent No. 170071 JP-A-9-30916 Japanese Patent Publication No. 6-693 Yoji Iikura, “Allergy / Immunity”, Vol. 8, No. 5, pp. 74-79, 2001

In view of the current situation, the present inventors have proposed a safety for reducing and / or recovering skin tissue inflammation caused by ultraviolet rays, active oxygen, aging, etc., and preventing and / or improving the skin troubles. In addition, we developed a stable material and provided an external skin product using this material.

In order to solve the above problems, the present inventors have made extensive studies on materials that reduce skin tissue inflammation caused by ultraviolet irradiation, and as a result, camellia is extremely effective in improving the skin trouble. Has found that it contains ingredients that can reduce inflammation caused by UV irradiation and can significantly improve skin aging symptoms, and that it can be effectively used in cosmetics and other external skin products. It came to do.

That is, according to the present invention, there is provided an anti-inflammatory agent comprising, as an active ingredient, an aqueous component of defatted camellia seeds of Camellia japonica belonging to the genus Camellia.

In this anti-inflammatory agent, the active ingredient is preferably an extract obtained by extracting defatted cocoons of camellia (Camellia japonica) seeds with water and / or a lower alcohol, and further, the aqueous component. It is even more desirable that the hydrolysis product of at least contain kaempferol. It is also desirable that anti-inflammation is based on the action of reducing the IL-1β concentration in the blood and / or is based on the action of increasing the TGF-β1 concentration in the blood or skin tissue. In the present invention, it is most desirable to satisfy both of these actions. It is more preferable to target inflammation caused by ultraviolet irradiation.

According to the present invention, there is also provided an external skin product for suppressing skin inflammation, comprising the anti-inflammatory agent. Here, it is desirable that the skin inflammation is caused by ultraviolet irradiation.

According to the present invention, skin inflammation caused by exposure to ultraviolet rays, further characterized by applying and / or contacting the skin with an aqueous component of camellia japonica seed defatted camellia belonging to the genus Camellia japonica A method for mitigating is provided. Here, the aqueous component is preferably an extract obtained by extracting defatted cocoons of Camellia japonica seeds with water and / or a lower alcohol, and further hydrolyzed product thereof. It is more desirable that at least contains kaempferol. In addition, the reduction of inflammation may be based on the action of decreasing the IL-1β concentration in the blood and / or the effect of increasing the TGF-β1 concentration in the blood or skin tissue. Desirably, those satisfying both of these effects are most desirable.

The aqueous component extracted from the camellia (Camellia japonica) seed defatted lees according to the present invention is excellent in quality stability and exhibits an action of reducing blood IL-1β concentration by applying it to the skin. And / or by exhibiting the effect | action which increases the TGF- (beta) 1 density | concentration in blood or skin tissue, there exists an effect which suppresses the inflammation of the skin by ultraviolet irradiation notably. And the external skin goods for reducing the inflammation of the skin formed by mix | blending the said aqueous component which concerns on this invention, especially the inflammation caused by ultraviolet irradiation are provided.

The present invention is described in detail below. First, the anti-inflammatory agent of the present invention has a function of reducing skin inflammation caused by exposure to ultraviolet rays, and the defatted pods of camellia (Camellia japonica) seeds belonging to the genus Camellia of Theaceae The aqueous component is contained as an active ingredient.

As the plant belonging to the camellia genus, generally, the camellia camellia camellia (Camelia japonica), the tea belonging to the tea section (C. sinensis), the sasanqua (C. sasanqua), etc. (C. granthamiana), etc., areowed sasanqua (C. salicifolia), and so on, and in the present invention, those that belong to camellia are known. Use. Examples of this are C. japonica var. Japonica, C. japonica subsp. Rusticana, C. japonica var. Macrocarpa, C. japonich. Hong Konggenesis, C. reticulata, C. salenensis, Pitaldi var. pitaldi, and C. partidi var. yunnica C, p. Yamabe camellia (same kind as Ayaba camellia), mountain tea flower (same kind as Ayers camellia), Kushimatsubaki can be mentioned (apple camellia and the like) and the like. What is necessary is just to utilize suitably these camellia which are growing naturally in the Japanese archipelago, the Korean peninsula, the Shandong peninsula of China, etc.

In the present invention, the above-mentioned camellia seeds are subjected to a compression treatment, an extraction treatment using a hydrophobic organic solvent such as hexane or heptane, or a liquefied gas such as liquefied carbon dioxide or liquefied propane, and a residue obtained by extracting oil by a conventional method. This is the essential raw material. Here, the camellia seeds may be derived from either early-ripening seeds or matured seeds, but it is preferable to use seeds from mature seeds from the viewpoint of defatted straw and the yield of active ingredients, including seeds or seeds and peels. The whole can be used. In the present invention, it is preferable to use matured whole seeds or seeds dried for about 1-2 weeks in the sun.

The aqueous component of the defatted lees can be produced by any method, but it is preferable to extract it with water and / or lower alcohol. The lower alcohol has a tendency to extract the oily substance in the defatted soot as the carbon number increases, so that a lower carbon number is desirable, such as methanol, ethanol, normal propanol, isopropanol, normal butanol, isobutanol, etc. It can be illustrated. When using a lower alcohol having a large carbon number, it is preferable to increase the water content in order to suppress the extraction of oily components in the defatted soot. For example, the water content in the case of propanol is about 20 wt% to about 50 wt%, and the water content in the case of butanol is about 40 wt% to about 70 wt%. Desirable extraction solvents are water, methanol and ethanol, and water-containing alcohols thereof (water content: 0 to 100% by weight).

In order to extract the defatted lees, the extraction solvent is added in an amount of about 1 to 30 times by weight with respect to 1 part by weight of the defatted lees, at about normal temperature or 1 to 5 atm. After stirring and mixing for 10 minutes to about 3 hours as necessary, the mixture is cooled to room temperature and filtered, and the filtrate is concentrated and dried by an appropriate means such as drying under reduced pressure, spray drying or freeze drying. The dried product may be appropriately pulverized. In this way, a light yellow to yellow solid which is an aqueous component of the defatted soot according to the present invention can be obtained. The extraction method is preferably performed by repeatedly extracting the extraction residue once extracted, or under a pressure of 1 to 3 atmospheres at about 100 ° C. to about 130 ° C. This increases the yield of the aqueous component according to the present invention. This aqueous component includes saponins, tannins, kaempferol, glycosides thereof and the like. That is, when the aqueous component according to the present invention is hydrolyzed, a product containing at least kaempferol is obtained.

The anti-inflammatory agent of the present invention contains the aqueous component as an active ingredient, and, as necessary, known excipients, binders, disintegrants, lubricants, diluents, etc. Alcohols, starches, celluloses, purified water and the like can be mixed and processed into a solid, paste or liquid form. The content of the active ingredient in the anti-inflammatory agent of the present invention is about 0.1 wt% to 100 wt%, more preferably about 10 wt% to about 80 wt%. If it is less than about 0.1% by weight, the desired effect may not be exhibited in an external skin product using the anti-inflammatory agent of the present invention.

In the external skin preparation of the present invention, the anti-inflammatory agent may be used as it is as an external skin preparation, but if necessary, a known additive used for an ordinary external skin preparation may be used in combination with a conventional method. It is desirable to prepare it as a composition. Here, the known additive may be any one that is used in pharmaceuticals, cosmetics, toiletry products, etc. and does not violate the gist of the present invention. For example, excipients, binders, disintegrants Various additives such as lubricants, wetting agents, fluidizing agents, preservatives, surfactants, stabilizers, diluents, solubilizers, bactericides, preservatives, colorants, and fragrances can be used.

Among the above-mentioned known additives, examples of excipients include cellulose and derivatives thereof, starch, modified starch, dextrin, resistant digestive dextrin, sugar alcohols such as lactose, mannitol, sorbitol, dicalcium phosphate, mica, talc, etc. Is mentioned.

Binders and disintegrators include cellulose derivatives such as crystalline cellulose, methylcellulose, ethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose acetate succinate, carmellose sodium, starch derived from wheat, rice, corn, potato, etc. Pregelatinized starch, partially pregelatinized starch, modified starch such as hydroxypropyl starch, dextrin, pullulan, polyvinylpyrrolidone, aminoalkyl methacrylate copolymer, methacrylic acid copolymer, polyvinyl acetal diethylaminoacetate, polyvinyl alcohol, gum arabic, agar, gelatin, white Examples include shellac, tragacanth, and macro goal.

As a lubricant, for example, starch derived from wheat, rice, corn, potato, stearic acid, calcium stearate, magnesium stearate, hydrous silicon dioxide, light anhydrous silicic acid, synthetic aluminum silicate, dried aluminum hydroxide gel, talc , Magnesium aluminate metasilicate, calcium hydrogen phosphate, anhydrous calcium hydrogen phosphate, sucrose fatty acid ester, waxes, hydrogenated vegetable oil, polyethylene glycol and the like.

Wetting agents, moisturizing agents, and emollients include squalane, squalene, lecithin, lysolecithin, cholesterol, sphingolipid, serine, glutamine, sorbitol, mannitol, glycerin, pyrrolidone carboxylic acid, 1,3-butylene glycol, propylene glycol, dipropylene Glycol, lactic acid and its salt, hyaluronic acid, sodium hyaluronate, collagen, water-soluble collagen, hydrolyzed elastin, alginic acid and its salt, mucopolysaccharide, polyethylene glycol, polyaspartate, water-soluble chitin, water-soluble chitosan, glucosamine And its derivatives, N-acetyl-D-glucosamine, cholesteryl long-chain acyl glutamate, cholesteryl hydroxystearate, hydrogenated castor oil, 12-hydroxystearic acid Ricinoleic acid, stearic acid, rosin acids, lanolin, lanolin fatty acid cholesteryl ester, isopropyl myristate, cetyl palmitate, isononyl isononanoate, silicone oils (cyclomethicone, dimethicone, cyclomethicone, etc.), can be exemplified milk whey, or the like.

Examples of the fluidizing agent include fine silicon dioxide, hydrous silicon dioxide, light anhydrous silicic acid, dry aluminum hydroxide gel, synthetic aluminum silicate, magnesium silicate and the like.

Antioxidants include ascorbic acid, tocotrienol, dl-α-tocopherol, α-tocopherol, γ-tocopherol, δ-tocopherol, tocopherols such as tocopherol acetate, isopropyl citrate, ethylenediaminetetraacetate calcium disodium, dibutylhydroxytoluene Phytic acid, caffeic acid, catechin, gallic acid, propyl gallate, erythorbic acid and its sodium salt, dilauryl thiodipropionate, L-cysteine hydrochloride and the like.

Examples of preservatives and preservatives include benzoic acid, sorbic acid, paraoxybenzoic acid, methyl paraoxybenzoate, propyl paraoxybenzoate, isopropyl paraoxybenzoate, propionic acid, sodium sulfite, chlorobutanol and the like.

Examples of surfactants include glycerophospholipids such as soybean lecithin, egg yolk lecithin, lysolecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, polyglycerin fatty acids such as sucrose fatty acid ester, glycerin fatty acid ester, diglycerin fatty acid ester Sorbitan fatty acid esters such as esters, sorbitan monostearate, sorbitan sesquioleate, polyoxyethylene higher alcohol ethers, polyoxypropylene higher alcohol ethers, polyoxyethylene fatty acid esters, polyoxypropylene fatty acid esters, polysorbate 80, polyoxyethylene sorbitan Nonionic surfactants such as fatty acid esters, polyoxyethylene hydrogenated castor oil, benzil chloride Cationic surfactants such as conium, stearyltrimethylammonium chloride, dicetyldimethylammonium chloride, and behenyltrimethylammonium chloride; amphoteric surface activities such as 2-cocoyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and betaine amidoacetate Agent, higher alcohol sulfate, higher alcohol ether sulfate, long chain fatty acid alkali metal salt, long chain fatty acid alkaline earth metal salt, long chain fatty acid basic amino acid salt, N-long chain acyl amino acid, N-long chain acyl amino acid There are anionic surfactants such as salts.

Examples of the stabilizer include paraoxybenzoates such as methylparaben and propylparaben, alcohols such as chlorobutanol, benzyl alcohol, and phenylethyl alcohol, benzalkonium chloride, acetic anhydride, sorbic acid, and the like.

Diluent, solubilizer, solubilizer, purified water, ethanol, lauryl sulfate triethanolamine, ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, glycerin, olive oil, castor oil, silicone oil, liquid paraffin, cyclodextrin, etc. is there.

Examples of isotonic agents include sodium chloride, potassium chloride, glycerin, boric acid and the like.

Examples of pH adjusters include lactic acid, succinic acid, fumaric acid, citric acid, malic acid, tartaric acid, gluconic acid, and sodium or potassium salts thereof, glucono delta lactone, adipic acid, sodium acetate, arginine, triethanolamine. Sodium hydroxide, potassium hydroxide, phosphoric acid, sodium dihydrogen phosphate, tripotassium hydrogen phosphate and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid derivatives such as paraaminobenzoic acid and octylparadimethylaminobenzoate, benzophenone derivatives such as 2-hydroxy-4-methoxybenzophenone and dihydroxydimethoxybenzophenone, ethyl paramethoxycinnamate, and octyl paramethoxycinnamate. Methoxycinnamic acid derivatives such as octyl salicylate, salicylic acid derivatives such as homomenthyl salicylate, α-dehydroamino acid derivatives such as N-benzoyl-O-methyl-α-dehydrotyrosine-2-ethylhexyl ester, 4- (3,4-dimethoxy) Benzalhydantoin derivatives such as phenyl) methylene-2,5-dioxo-1-imidazolidinepropionic acid 2-ethylhexyl ester, urocanic acid, ethyl urocanate, 4-ter-butyl -4'-methoxydibenzoylmethane, 2- (2'-hydroxy-5'-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, triclosan, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc pyrithione and the like.

Examples of colorants include zinc oxide, titanium oxide, iron oxide, silica, talc, mica, copper chlorophyll, water-soluble anato, β-carotene, riboflavin and its butyrate, gardenia yellow, blue No. 1, red No. 202, edible Examples include Red No. 2, No. 105, Edible Yellow No. 4, Edible Green No. 3, Edible Blue No. 2, and the like.

In addition, various fragrances and plant essences can be used as necessary, and as fats and oils, vegetable oils such as avocado oil, olive oil and jojoba oil, fatty acids such as oleic acid and isostearic acid, petrolatum, lanolin, ceresin, microcrystalline wax, Carnauba wax, candelilla wax, coconut oil fatty acid, lauric acid, hardened beef tallow fatty acid and other fatty acids, cetyl 2-ethylhexanoate, isopropyl myristate, 2-octyldodecyl myristate, neopentyl glycol di-2-ethylhexanoate, Ester oil such as glycerol tri-2-ethylhexanoate, oleic acid-2-octyldodecyl, glycerol triisostearate, 2-ethylhexanoic acid diglyceride, ester oil such as long chain acyl glutamic acid octyldodecyl ester, Siloxane, methyl hydrogen polysiloxane, methylphenyl polysiloxane, silicone oils such as octamethylcyclotetrasiloxane, liquid paraffin, can be used squalene, etc. in appropriate liquid hydrocarbon oils squalane. In addition, this invention is not limited at all by these each illustration.

The form of the external skin product of the present invention is not particularly limited, and is generally applied to the skin, hair and scalp, and is a dosage form such as a lotion, emulsion, gel, cream, ointment, etc. For example, lotion, emulsion, cream, foundation, pack, essence, lipstick, face wash, shampoo, rinse, hair tonic, hair treatment and the like can be mentioned. In addition, quasi-drugs such as ointments, poultices, bath preparations, cleaning agents and aerosols may be included.

In order to produce the skin external product of the present invention, the above-mentioned known additives are appropriately selected, and a predetermined amount of the above-mentioned aqueous component according to the present invention is added thereto, followed by processing by an ordinary production method. Here, the blended amount of the aqueous component according to the present invention is about 0.01 wt% to about 90 wt%, more desirably about 0.1 wt% to about 70 wt%. If the amount is less than about 0.01% by weight, the external skin product of the present invention may not exhibit the desired effect, and if it exceeds about 90% by weight, it may be difficult to process a normal dosage form as an external skin product. In order to prevent and / or ameliorate skin irritation, particularly skin irritation caused by exposure to a large amount of ultraviolet rays and / or over a long period of time, the external skin product of the present invention is used for its intended purpose. It can use suitably by the method of apply | coating to or contacting.

Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited at all by this. In each example, all the percentages, parts and ratios are based on weight.

Production Example 1
After coarsely crushing dried seeds of C. japonica var. Japonica from Goto, Nagasaki Prefecture, steaming and then pressing to obtain a press knead separated from the compressed oil, followed by adding normal hexane to the press koji and extracting by a conventional method After processing, the extract was separated and the extract was collected. The extracted soot was washed with normal hexane to remove oil, and defatted soot was collected. 400 mL of water was added to 100 g of this defatted soot, heated to 85 ° C. under normal pressure and stirred for 1 hour as appropriate, then cooled to room temperature and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 15.8 g of a powder (referred to as sample 1) containing an aqueous component according to the present invention. When this powder was hydrolyzed and analyzed by HPLC, it contained 15.2% sapogenin, which is an aglycon of saponin, and 2.8% kaempferol, which is a kind of flavonol.

Production Example 2
The dried seeds of Yakushima apple camellia (C. japonica var. Macrocarpa) were defatted by the method described in Production Example 1 and defatted cocoons were collected. 400 mL of water was added to 100 g of the defatted soot, and the mixture was heated at 120 ° C. for 25 minutes under a pressure of 2 atm, then cooled to room temperature and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 16.4 g of a powder containing the aqueous component according to the present invention (referred to as sample 2). The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 13.7% and the kaempferol content was 2.2%.

Production Example 3
300 g of water-containing ethanol (water content 35%) was added to 100 g of the defatted lees obtained by the method described in Production Example 1, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. To this filtration residue, 200 mL of water-containing ethanol (water content 35%) was added again and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 11.3 g of powder (referred to as sample 3) containing an aqueous component according to the present invention. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 11.3% and the kaempferol content was 2.7%.

Production Example 4
Ethanol (purity 99.5%) 300 mL was added to 100 g of the defatted lees obtained by the method described in Production Example 2, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. 200 mL of ethanol (purity 99.5%) was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 4.5 g of a powder (referred to as sample 4) containing an aqueous component according to the present invention. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 13.0% and the kaempferol content was 2.7%.

Test example 1
The effect of the powder (Sample 1 to Sample 4) containing the aqueous component according to the present invention on the reduction of skin inflammation caused by ultraviolet exposure was examined by the following method. That is, using hairless mice (HR-1 / Hos, male, 7 weeks old, 36 (6 mice per group)), ultraviolet irradiation (UV-B, intensity: 30 mJ / cm ² ) on a certain area of the back part, ATTO UV lamp was used) once a day at a rate of 1 minute per time for 7 days. During this time, 0.5 ml each of test substance (sample 1 to sample 4) aqueous solution or purified water dissolved in purified water at a concentration of 0.1% immediately after UV irradiation is soaked in 5 x 4 cm sterile gauze and applied to the back. did.

The degree of inflammation at the ultraviolet irradiation site was determined by visual observation. In addition, IL-1β as an inflammatory cytokine and TGF-β1 as an anti-inflammatory cytokine were measured. IL-1β concentration and TGF-β1 concentration in blood were collected by collecting mouse blood in a centrifuge tube to which EDTA was added, and collecting plasma by centrifugation (4 ° C., 3000 rpm, 20 minutes). IL-1β (manufactured by BIO SOURCE), TGF-β1 (manufactured by PROMEGA)). The TGF-β1 concentration in the skin tissue was determined by collecting the skin tissue on the back after slaughtering the mouse, suspending it in a buffer solution in an ELISA kit, homogenizing under cooling, and then centrifuging (room temperature, 2000 rpm). 15 minutes), the supernatant was recovered, and the TGF-β1 concentration in the supernatant was measured using an ELISA kit (manufactured by PROMEGA).

The results are shown in Table 1. In the table, the numerical value is shown as a relative value when the value of the ultraviolet ray non-irradiation test (when no ultraviolet ray is irradiated) carried out at the same time is taken as 100. In addition, the degree of inflammation at the site irradiated with ultraviolet rays was expressed as follows: no inflammation:-, slightly red spots: +, red spots and rough skin: ++, rough skin / wrinkle formation: ++.

As a result, in the control group not irradiated with ultraviolet rays, there was no inflammation and no wrinkle formation or rough skin was observed, but in the positive control group irradiated with ultraviolet rays, clear red spots, wrinkle formation and rough skin were generated. . On the other hand, in each of the groups where each sample was applied while being irradiated with ultraviolet rays, red spots, wrinkle formation and rough skin caused by ultraviolet irradiation were almost halved. From this, it was confirmed that the said aqueous component which concerns on this invention has the skin aging prevention effect which can reduce the skin trouble by ultraviolet irradiation. In addition, IL-1β, which is an inflammatory cytokine, was increased by ultraviolet irradiation, but in each group to which each sample was applied, the increase in IL-1β was reduced, and inflammation was reduced. On the other hand, TGF-β1, which is an anti-inflammatory cytokine, was increased by ultraviolet irradiation, but in each group to which each sample was applied, the increase in TGF-β1 was further increased, and the tissue repair function due to inflammation was increased. It has been suggested that is increasing.

Test example 2
An oil phase component consisting of squalane: 10 parts, stearic acid: 5 parts, cetanol: 3 parts and stearic acid monoglyceride: 3 parts was heated and dissolved at 80 ° C., while glycerin: 15 parts, methyl paraoxybenzoate: 0. 1 part, carboxyvinyl polymer (1% aqueous solution): 10 parts, polyglycerol (average polymerization degree 4) monooleate: 3 parts, arginine (20% aqueous solution): 15 parts, sample 2: 1 part and purified water: 34 The aqueous phase component consisting of 9 parts was dissolved by heating at 80 ° C. The oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer to prepare a test cream. Further, in this cream, a comparative cream (adjusted with purified water) not containing Sample 2 was prepared. 10 volunteer healthy adult women (22-54 years old, average age: 31.0 years old) apply the test cream to the left hand and the comparative cream to the right hand twice a day for 2 months (7 (Month-August) We had daily life and conducted a questionnaire survey (three-level evaluation) on the effects on the skin.

As a result, (1) Regarding the occurrence of erythema and redness on the skin, there were no comparison claims: 5 people, slight erythema or redness: 4 people, obvious redness: 1 people, for testing No cream: 8 people, slight erythema: 2 people. (2) About the roughness and roughness of the skin, the comparison cream is strong: 3 people, normal: 5 people, weak: 2 people, and the test cream is strong: 0 people, normal: 4 people, weak: 6 people there were. From this, by continuously applying the cream containing the aqueous component according to the present invention to the skin, the occurrence of erythema and redness of the skin is reduced in summer, and it is also effective in preventing roughness and rough skin. It was confirmed.

Prototype examples 1-7
Various types of external skin products were made using the following formulation (all values are% by weight).
(1) Lotion (a) Sorbit 2
(B) 1,3-butylene glycol 4
(C) Polyethylene glycol 1000 2
(D) Ethanol 10
(E) Sample 1 1
(F) Preservative (Methylparaben) Appropriate amount (g) Purified water The remaining amount (a) to (f) is added to (g) heated to 80 ° C., stirred and dissolved, then cooled to room temperature and filled into a container did.

(2) Latex (a) Squalane 4
(B) Vaseline 1
(C) Stearyl alcohol 0.5
(D) Sorbitan monostearate 1.5
(E) Polyoxyethylene (20) sorbitan monooleate 3
(F) 1,3-butylene glycol 5
(G) Sample 2 0.1
(H) Tea catechin 0.1
(I) Purified water The remaining amounts (a) to (d) were heated and dissolved at 80 ° C. to obtain an oil phase component, and (e) to (i) were heated and dissolved at 80 ° C. to obtain an aqueous phase component. At the same temperature, the oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer, cooled to room temperature, degassed, and filled into a container. This emulsion can be used to suppress skin inflammation.

(3) Cream (a) Squalane 20
(B) Beeswax 5
(C) Jojoba oil 5
(D) Sorbitan monostearate 1
(E) Glycerol monooleate 3
(F) Polyoxyethylene (20) sorbitan monostearate 3
(G) Glycerin 5
(H) Sample 3 5
(I) N-acetylglucosamine 2
(J) Purified water The remaining amounts (a) to (e) were heated and dissolved at 80 ° C. to obtain an oil phase component, and (f) to (j) were heated and dissolved at 80 ° C. to obtain an aqueous phase component. At the same temperature, the oil phase component was added to the aqueous phase component with stirring, and the mixture was uniformly emulsified with a homogenizer, cooled to room temperature, degassed, and filled into a container. This cream can be used as an external skin preparation for anti-inflammation.

(4) Body soap (a) Potassium laurate 13
(B) Potassium myristate 5
(C) Propylene glycol 7
(D) Sample 4 10
(E) Hibiscus flower extract 3
(F) pH adjuster (citric acid) appropriate amount (g) preservative (methyl paraben) appropriate amount (h) purified water The remaining amount (a) and (b) were dissolved in advance by heating at 80 ° C. (c) to (h) The mixture was added to the solution with stirring and mixed uniformly with a homogenizer, then cooled to room temperature, degassed and filled into a container. This body soap can be used as an external skin product for reducing skin inflammation and preventing rough skin.

(5) Shampoo (a) Lauric acid diethanolamide 2
(B) Lauryl sulfate triethanolamine 5
(C) Polyoxyethylene lauryl ether sodium sulfate 12
(D) 1,3-butylene glycol 4
(E) Edetate disodium 0.1
(F) Sample 2 0.05
(G) Preservative and perfume appropriate amount (h) Purified water The remaining amount (b) to (h) is heated and dissolved to 70 ° C., (a) is added, emulsified with a homomixer, cooled and deaerated. Filled into a container.

(6) Hair tonic (a) Ethyl oleate 1
(B) Ethanol 45
(C) Polyoxyethylene (40) hydrogenated castor oil 2
(D) Sample 1 15
(E) Purified water The remaining amount (c) to (e) are dissolved by heating to 60 ° C., the mixture of (a) and (b) heated to the same temperature is added, solubilized by a homomixer, cooled, Degassed and filled into containers.

(7) Powder bath agent (a) Sodium bicarbonate 50
(B) Anhydrous sodium sulfate 35
(C) Borax 3
(D) Sample 3 7
(E) Chamomile extraction powder 4
(F) Colorant and fragrance Each appropriate amount (a) to (f) was mixed with a powder mixer and filled into a container. This powder bath preparation can be used for bathing and soothing erythema and hot flashes on the skin.

Aqueous components obtained from camellia seed defatted have the effect of reducing skin irritation caused by exposure to ultraviolet rays and suppressing rough skin and roughness. It can be used as an external skin product that restores the physiological function of the skin, helps improve skin troubles, and recovers early skin damage.

Claims (3)

  Skin caused by ultraviolet irradiation, characterized by containing, as an active ingredient, hot water (85 ° C. or higher) extract under normal pressure or pressure of defatted camellia of Camellia Japan, which belongs to the camellia family Camellia Of producing a TGF-β1 potentiator for suppressing inflammation of the skin.   The method for producing a TGF-β1 enhancer according to claim 1, wherein the hydrolyzed extract contains at least sapogenin and kaempferol. After passing through the production method according to claim 1 or 2, a TGF-β1 potentiator obtained by the production method is blended, and is used to suppress skin inflammation caused by ultraviolet irradiation. A manufacturing method of goods.