KR20140057437A - Cosmetic composition for preventing fall-out of hair, growing hair, preventing of generating dandruff and relieving inflammation of scalp - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss, promoting hair growth, preventing dandruff generation, and alleviating scalp inflammation. The cosmetic composition of the present invention comprises, as an active ingredient, a composite composition including a Pleuropterus multiflorus TURCZ. extract, a Sophora flavescens Solander ex Aiton extract, a licorice extract, insulin like growth factor-1 (IGF-1), platelet derived growth factor (PDGF), and methyl sufonyl methane (MSM). Accordingly, hair loss is prevented, hair growth is promoted, dandruff generation is prevented, and scalp inflammation is alleviated, so that scalp condition is improved and hair loss is alleviated.

Description

[0001] The present invention relates to a cosmetic composition for preventing hair loss, promoting hair growth, inhibiting dandruff formation and alleviating scalp irritation, and more particularly, to a cosmetic composition for preventing hair fall-

The present invention relates to a cosmetic composition for preventing hair loss, promoting hair growth, inhibiting dandruff formation and alleviating scalp irritation.

Hair is an important part of the day as the interest in the appearance increases. However, there are increasing numbers of people who complain of hair loss due to various factors such as dietary changes and internal and external stress. In addition to protecting the scalp, such as UV protection and buffering action, hair is also important in terms of cosmetics that determine the impression and appearance of a person. Hair loss is caused by genetic factors and changes in social and cultural factors that may have direct or indirect effects, such as increased stress, nutritional imbalance due to Westernized eating habits, and increased social activities of women.

Hair is produced in hair follicles, a skin-implanted structure with continuous proliferation of stromal cells at the base of the scalp, and has hair cycles involving various growth stages (Reynolds et al. 1991). In degeneration, apoptosis of hair follicular keratinocyte occurs, pigmentation is discontinued, and extracellular matrix is reconstituted. In addition, the contraction occurs as a result of the dermal papilla is the hair follicle degeneration (Piao YJ, Seo EY, Kim JS. The Influence of Testosterone PRopionate on the Expression of Seceral Growth Factors in Human Scalp Dermal Papilla Cell. Joirnal of Investigative Dermatology . 2002: 9: 29-34).

For example, epidermal growth factor (EGF), transforming growth factor-a (TGF-a), transforming growth factor-b (TGF-b), and keratinocyte growth factor, insulin like growth factor (IGF), interleukin-1, basic fibrovlast growth factor (bHGF) and vascular endothelial growth factor (VEGF) (Wagana P, Haikou M, Klepetsanis P, Giannopoulou E, Ioannou PV, Sophia GA. In vivo distribution of arsonoliposomes: Effect of vesicle lipid composition. Journal of Pharmaceutics . 2008: 347: 86-92).

The causes of hair loss include male hormone hyperactivity, excess sebum secretion, poor blood circulation, peroxidation, hypofunction of the scalp, genetic factors, aging, and stress. It is not revealed.

Free radicals that cause oxidative stress are produced by internal factors such as chemical reactions of various compounds and various oxidation and reduction reactions, and external factors such as smoking, drinking, stress and ultraviolet rays (Halliwell, B., Antioxidant .... in human health and disease Annu Rev Nutr 16: 33-49, 1996; Morrissey, PA and O'brien, NM (1998) Dietary anti-oxidants in health and disease Int Dairy J. 8:... 463 -472), the reactive oxygen species (ROS) generated by free radical reaction in the upper body causes the inactivation and peroxidation reaction of DNA segment and protein, which causes lowering of upper body function, is (Valko, M, Leibfritz, D. , Moncol, J., Cronin, MT, Mazur, M and Telser, J, 2007;..... Free radicals and antioxidants in normal physiological functions and human disease Int J. Biochem Cell Biol . 39: 44-84).

Studies on the direct effects of reactive oxygen species on male hair loss are inadequate, but in some cases they are a major cause of worsening hair loss. For example, it has been reported that the contents of enzymes such as superoxide dismutase and glutathione peroxidase, which inhibit lipid peroxidation in patients with round hair loss, are higher in the scalp than in normal subjects. This means that oxidative stress affects hair loss (Ahmet A, Ercan A, Hakan E. Antioxidant enzymes and lipid peroxidation in the scalp of patients with alopecia areata. Journal of Dermatological Science . 2002: 29: 85-90; Rafet K, Ferah A. Evaluation of lipid peroxidation, oxidant / antioxidant status, and serum nitric oxide levels in alopecia areata. Medicine Science Monit . 2005: 11: 296-299).

Minoxidil and finasteride are well known as FDA-approved drugs that promote hair growth. The mechanism of action of minoxidil on the hair growth effect has not been clarified yet, but it is thought that the increase of nutritional supply through vasodilation, the effect of potassium channel opening and the suppression of apoptosis of dermal papilla cells are thought to induce hair growth (Han JH , et al., Effect of minoxidil of proliferation and apoptosis in dermal papilla cells of hyman hair follicle., J Dermatol Sci , 34, pp. 91-98, 2004).

Hair cosmetics compositions having use for hair loss or hair growth have been widely used. In the case of commercially available products, in many cases, the main ingredient is a chemical substance, which often causes side effects on the scalp. In addition, when the FDA-approved minoxidil is applied as a therapeutic agent for alopecia, unexpected side effects such as reduction of sexual function due to unbalance of body enhancement, allergy, depression, and local inflammation may occur.

In order to prevent the side effects caused by the chemical components, researches and inventions of cosmetic compositions for depilation or hair growth using natural extracts are under way. For example, an anti-hair restoration composition containing herbal extracts containing herb extracts containing ganoderma, ganoderma, sesame, mandarin, ganoderma and gum (Korean Patent Laid-Open Publication No. 10-2009-51586); (Korean Patent Laid-Open Publication No. 10-2005-14473), which has a fungicide-disinfecting function and contains Baekgyeong, Daesan, and Golden extract, has been filed and disclosed. However, conventional cosmetic compositions which are conventionally distributed have virtually no products having satisfactory effects for preventing hair loss or preventing dandruff.

As described above, the cause of hair loss varies. If the nutrients that can constitute the hair are not properly supplied due to the blood circulation disorder due to the capillary blood vessel lesion or compression, or if the physiological substance is accumulated excessively, resulting in the abnormality of the osmotic pressure homeostasis, In the case of hair follicles, there are numerous causes such as apoptosis caused by abnormality of hair follicle cycle, keratin due to excess sebum secretion, deterioration of scalp environment due to wastes, degradation of scalp function due to bacteria, and so on, The problem of hair loss can not be solved. Therefore, there is a need for composites capable of improving various causes at the same time and synergizing with hair loss alleviating effect and scalp environment improving effect.

Accordingly, the inventors of the present invention have invented a cosmetic composition which is capable of improving scalp health while preventing hair loss, promoting hair growth, inhibiting dandruff formation and alleviating scalp inflammation, and free from various side effects.

Accordingly, it is an object of the present invention to provide a cosmetic composition which can inhibit hair loss and promote hair growth by having a 5α-reductase inhibitory activity, a cell activity effect and / or an antioxidative activity.

It is also an object of the present invention to provide a cosmetic composition capable of inhibiting the production of dandruff by inhibiting dandruff formation.

It is also an object of the present invention to provide a cosmetic composition capable of alleviating inflammation of the scalp, having anti-inflammatory activity.

Another object of the present invention is to provide a cosmetic composition having excellent moisture retention and skin stability.

According to the present invention, the above object can be accomplished by providing a complex composition comprising Sucrose extract, Gossam extract, licorice extract, insulin like growth factor-1 (IGF-1), Platelet derived growth factor (PDGF) and MSM (Methyl Sufonyl Methane) And can be achieved by a cosmetic composition for preventing hair loss and promoting hair growth containing the active ingredient.

According to the present invention, the above objects can also be accomplished by providing a complex composition comprising a mushroom extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF), and a methylsufonyl methane As an active ingredient, to the dandruff formation inhibiting cosmetic composition.

According to the present invention, the above objects can also be accomplished by providing a complex composition comprising a mushroom extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF), and a methylsufonyl methane As an active ingredient, to a cosmetic composition for relieving scalp irritation.

0.001 to 20% by weight, respectively, of the Sucrose extract, the Gossam extract, the licorice extract and the MSM, relative to the composite composition; Each of IGF-1 and PDGF may contain 0.001 to 15% by weight.

The extracts include water containing purified water, lower alcohols having 1 to 4 carbon atoms, nonpolar solvents, or extracts soluble in a mixed solvent thereof.

The composite composition may contain 0.0001 to 10% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may further comprise a moisturizing agent, and the composite composition may be contained in an amount of 0.01 to 0.5 times the amount of the moisturizing agent added.

The moisturizing agent may be contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition, and the composite composition may include 0.001 to 10% by weight.

The humectant may include dilauroyl glutamate lysine or a non-toxic salt thereof.

The cosmetic composition may be at least one selected from the group consisting of a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair conditioner, a hair treatment, a hair cream, Hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair fresheners, , Hair mousse or hair spray.

INDUSTRIAL APPLICABILITY As described above, the cosmetic composition according to the present invention may have a 5α-reductase inhibitory activity, a cell activity effect and / or an antioxidative activity, thereby promoting hair loss prevention and hair growth.

In addition, the cosmetic composition according to the present invention may have an effect of inhibiting dandruff formation because of its activity to inhibit dandruff formation.

In addition, the cosmetic composition according to the present invention has an anti-inflammatory activity and thus may have an effect of alleviating inflammation of the scalp.

In addition, the cosmetic composition according to the present invention has an excellent moisturizing ability and a good skin stability, so that side effects can be minimized.

FIG. 1 is a graph showing an antioxidant effect test result of the composite composition according to the present invention,
FIG. 2 is a graph showing experimental results of cell activity of the composite composition according to the present invention,
FIG. 3 is a graph showing the experiment result of the 5α-reductase inhibitory effect of the composite composition according to the present invention,
Figures 4 and 5 are graphs showing the anti-inflammatory effects of the composite composition according to the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

The present invention relates to a pharmaceutical composition comprising as an active ingredient a complex composition comprising a mushroom extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF), and a methylsufonyl methane The present invention provides a cosmetic composition for preventing hair loss and promoting hair growth.

The present invention also provides a pharmaceutical composition comprising a complex composition comprising a mushroom extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF) and a methylsufonyl methane (MSM) And a cosmetic composition for inhibiting dandruff formation.

The present invention also relates to a pharmaceutical composition containing a complex composition comprising a mushroom extract, a ginseng extract, a licorice extract, insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF) and a methylsufonyl methane (MSM) A cosmetic composition for relieving scalp irritation.

Polygonumultiflori Radix is an endophytic plant that contains anthraquinone and stibene derivatives. Emodin, one of the anthraquinone compounds, has been reported to have antibacterial, anti-inflammatory, antioxidant, and anti-cancer effects (Hatano et al. 1999). (Yin et al., 1992). In addition, there is the effect of reducing blood sugar and blood lipid content, as well as protecting liver and kidneys and producing blood. Yin et al. (Wang et al., 1988; Lee et al., 2003).

Gosam (Sophora flavescens Aiton) is circumferential and its outer surface is dark brown or tan, has a vertical wrinkle, and has a long, thick skin. When peeled off, it is yellowish white and the folded surface is slightly fibrous. Gosam is used for dysentery due to wet heat of the wastewater, subarachnoid pain, itching of the skin and itching of the skin. It is used when the urine is not visible due to bladder fever and the pain is present. Pharmacological effects include leukopenia treatment, anti-radioactivity, increased coronary blood flow, cardiac muscle strengthening, hypoglycemic action, antitumor, antimicrobial and immunosuppressive action (Source: Doosan Encyclopedia).

Licorice (Glycyrrhiza uralensis Fischer) is a perennial herb that belongs to soybean and contains 6 ~ 14% of glycyrrhizin, a sweet component. It has been used for almost all herbal ingredients and its safety has long been proven. Glycyrrhizin, which is the main component of licorice, is known to be effective for allergy, chronic hepatitis and viral diseases by saponin system and has been widely used for foods, medicines and cosmetics because of its whitening effect. Licorice is also the root of glycyrrhiza plant, which has been used for over 4,000 years and is a Chinese herb used as an expectorant, to stop grapevines, to lower fever, to relax the stomach, and to ease the emergency situation. And increases the effectiveness of various other herbs.

Insulin like Growth Factor-1 (IGF-1) is known to form bones, muscles, nerves, blood vessels, tissues, help regenerate damaged cells and inhibit apoptosis (DL Roith. Et al., Dev . Biol 1980; 75: 315).

Platelet derived growth factor (PDGF) is a platelet-derived growth factor, a dendritic protein in which two long polypeptide chains are linked by a sulfide bond. PDGF contains three double-stranded proteins, PDGF-AA, PDGF-BB and PDGF-AB, by the expression of individual independent genetic qualities. In particular, the PDGF-A factor is expressed in mesenchymal cells and endothelial cells, osteoblasts, chondrocytes and osteoclasts in the late osteoporosis healing. The growth factor is defined as a polypeptide having mitotic activity and is secreted in cells in which it works or secreted in adjacent cells and synthesized in various tissues and cells. The PDGF is one of the growth factors involved in wound healing . PDGF was detected by Ross et al. (1974) and Kohler and Lipton (1974) by the fact that substances that stimulate the proliferation of arterial smooth muscle cells were secreted from the platelets. Antoniades et al. (1979) PDGF has been reported to be found not only in platelets but also in mononuclear cells, macrophages, endothelial cells, and bone matrix, and the origin of PDGF is the alpha granule of platelets. PDGF has been shown to be involved in the differentiation of mesenchymal cells such as the cytoplasm, smooth muscle cells, And PDGF is an important cytoplasmic cleavage promoting factor that regulates bone metabolism because it acts as a chemoattractant factor that induces osteoblast migration to bone remodeling sites. According to Hicklin's (1994) In the cell concentration culture, the synthesis of DNA synthesis rate, number of cells, collagen and non - collagen protein were increased. In cultured chondroblast, DNA synthesis and salt Owen et al. (1982) reported that when PDGF acts on human diploid fibroblasts, it increases DNA synthesis and total protein content (Jung, Sung-Wook Chung, "Freezing of Platelet-derived Growth Factor The effect of dry allogeneic demineralized bone on tissue healing during transplantation of skull defects in white rats ", 2003). The PDGF used in the present invention may be, but not limited to, PDGF-A.

MSM (methylsulfonylmethane) is a dietary sulfur that is concentrated in pine trees (sea horses). 34% of the total mass is sulfur, which is one of the most sulfur-containing substances in natural products. It is also known that dietary sulfur is a white, odorless crystal that is not toxic and is used as a sulfur source in animal feeds and has various functions in the body (Jang HD, Yoo JS, Chae SJ, Park SL, Kim IC, Park JC , Jung HJ, Kim YH, Kim IH, Seok HB. 2006. Effects of dietary of methyl sulfonyl methane on growth performance and meat quality characteristics in growing-finishing pigs. Korean J Intl Agric 18: 116-120). The known function of sulfur is a component of sulfur-containing sulfur amino acids (methionine, cysteine), a component of glutathione that acts antioxidantly and detoxifyingly, is a component of collagen and glycoprotein, promotes collagen synthesis, And is essential for the action of steroid hormones and various growth factors (Jang HD, Yoo JS, Chae SJ, Park SL, Park JC, Jung HJ Kim YH, Kim IH, Seok HB 2006. Effects of dietary methyl sulfonyl methane on growth performance and meat quality characteristics in growing-finishing pigs. Korean J Intl Agric 18: 116-120; Cho JH, Min BJ, Kwon OS, Shon KS, Jin YG, Kim HJ, Kim IH. 2005. Effects of MSM (methyl sulfonyl methane) supplementation on growth performance and digestibility of Ca and N in pigs. J Korean Soc Food Sci Nutr 34: 361-365).

MSM (methylsulfonylmethane) is a non-toxic odorless mineral which has strong odor and toxicity and is widely used in nutritional supplement, health supplements and cosmetics. It is used for pain relief, inflammation relief, vascular expansion and blood flow enhancement, (Yung CS, Choi HG, Jang SW, Yoo BK. 2009. The effect of methylsulfonylmethane on hair growth promotion of magnesium ascorbyl phosphate for the treatment of Alopecia. Biomolecules & Therapeutics , 17 (3) 241-248).

0.001 to 20% by weight, respectively, of the Sucrose extract, the Gossam extract, the licorice extract and the MSM, relative to the composite composition; Each of IGF-1 and PDGF may contain 0.001 to 15% by weight. Preferably, 0.001 to 15% by weight of the extract of Sasa extract, 0.001 to 15% by weight of the extract, 0.001 to 15% by weight of the licorice extract, 0.001 to 15% by weight of the MSM, 0.001 to 10% And 0.001 to 10% by weight of the PDGF.

Each of the above-mentioned Sasa extract, Gossam extract, and Licorice extract includes water containing purified water, a lower alcohol having 1 to 4 carbon atoms, a non-polar solvent, or an extract obtained by using the mixture in a mixed solvent thereof. Water, and water; and drying and pulverizing each of the above-mentioned water, ginseng and licorice; and water of about 0.5 to 20 parts, preferably about 1 to 15 parts by weight of the sample; Lower alcohols having 1 to 4 carbon atoms such as ethanol, methanol, butanol, propanol, butylene glycol, propylene glycol and the like; Chloroform, ethyl acetate, dichloromethane, hexane, petroleum ether, diethyl ether or the like or a mixed solvent thereof at room temperature for about 0.1 to 48 hours, preferably 0.1 to 30 hours, Extraction with supercritical extraction, filtration, concentration under reduced pressure or freeze-drying to obtain the extract according to the present invention.

The composite composition may contain 0.0001 to 10% by weight, preferably 0.001 to 5% by weight, based on the total weight of the cosmetic composition.

The cosmetic composition may further comprise a moisturizing agent, and the composite composition may be contained in an amount of 0.01 to 0.5 times the amount of the moisturizing agent added.

The moisturizing agent may contain 0.01 to 20% by weight, preferably 0.01 to 10% by weight, and the composite composition may contain 0.001 to 10% by weight, preferably 0.001 to 5% by weight, based on the total weight of the cosmetic composition.

The humectant may include dilauroyl glutamate lysine or a non-toxic salt thereof. The dilauroyl glutamate lysine has a peptide skeleton (Glu-Lys-Glu) in which two sodium lauroyl glutamate sodium salt with 1 chain hydrophilic is linked by lysine as a space, and shows gemini type amphipathic (ROSEN M, Geminis J., A New Generation of Surfactants, CHEMTECH , 23, 30, 1993). Or moisturizers that may be included in common cosmetic compositions, for example, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, and the like.

The cosmetic composition according to the present invention can optionally add additives that can be formulated into cosmetic compositions for scalp and hair. For example, stabilizers, solubilizers or emulsifiers, vitamins, surfactants, thickeners, pH adjusters, preservatives, antioxidants, pigments, fragrances, or combinations thereof may be further added.

Stabilizers may include butyl ethylhexanoyl glutamide, dibutyl lauroyl glutamide, and the like.

The solubilizing or emulsifying agent may include isopropyl myristate, polyethylene glycol, triglycerides of medium chain fatty acids, hydrocarbons, glycols and the like.

Specific examples of the anionic surfactant in the surfactant include ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isocyanate, sodium lauryl isethionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium Lauryl ether sulfate (1 to 3 ethylene oxide), and the like. The nonionic surfactant in the surfactant may specifically be polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, hardened castor oil derivative, fatty acid diethanolamide, glyceryl stearate, and the like. The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like. The amphoteric surfactant in the surfactant may be specifically betaine, amide betaine, sulfobetaine, stear trimonium chloride, and the like.

The thickening agent may specifically be a water-soluble polymer compound such as methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxymethyl cellulose, hydroxymethyl cellulose and the like.

The pH adjuster may specifically be citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid, and the like.

Preservatives may specifically be benzoic acid, paraoxybenzoic acid esters, mixtures of methylchloroisothiazolinone, phenoxyethanol, DMDM, and the like.

The antioxidant may specifically be dibutylhydroxytoluene, ascorbic acid, and the like.

As the pigment and the fragrance, the components generally used in cosmetic compositions for scalp and hair may be used.

The cosmetic composition according to the present invention can be prepared into any formulation conventionally produced in the art to which the cosmetic for scalp and hair belongs. For example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, shampoos, surfactant-containing cleansing, tonics, scaling, and the like. For example, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massager cream, hair wax, hair aerosol Hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, A hair mousse or a hair spray.

When the formulation of the cosmetic composition according to the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, carboxylvinyl polymer, propylene glycol, 1,3-butylene glycol , Glycerin aliphatic esters, polyethylene hardened castor oil, preservatives or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, the carrier component may include water, a corynebum, a liquid diluent such as glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester , Microcrystalline cellulose, carboxyvinyl polymer, thickening agent or preservative may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfate, aliphatic alcohol ester sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether An alkylamidobetaine, an aliphatic alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a lanolin derivative, or an ethoxylated glycerol fatty acid ester.

Hereinafter, the present invention will be described more specifically with reference to Examples and Experimental Examples.

Example  1. Preparation of Composite Composition According to the Invention

1-1. Preparation of extract

Each of the samples was dried and pulverized, and 60 g of each sample was placed in a vacuum bag and 540 g of water was added thereto, followed by vacuum treatment using genuine air (IS-100, Namwoong, Korea). After that, the product was subjected to a 2-liquid-liquid super-high pressure treatment using an ultrahigh pressure apparatus (TFS-2L, TOYO KOATSU CO., LTD., Japan) under the conditions of 100 MPa (1,000 bar), 25 ° C. and water (buffer solvent) for 1 hour. After the ultrahigh pressure treatment was completed, the samples were centrifuged at 8,000 rpm and 15 ° C for 20 minutes using a centrifuge (Supra 22K, Hanil, Korea) to separate the extracts of Hwasoo, Gosam and licorice. The thus isolated extract was filtered with a 0.8 占 퐉 or 0.45 占 퐉 filter to obtain a filtrate. The filtrate was subjected to freeze pretreatment at -21 占 폚, followed by drying at -80 占 폚 for 30 minutes using a freeze dryer (Bondiro, Ilshin, Korea) Followed by lyophilization for 48 hours to obtain extracts of Dassuo, Gosam and Licorice, respectively.

1-2. Preparation of Composite Composition

(I), 0.001 to 15% by weight of the Sasa extract prepared in Example 1-1, 0.001 to 15% by weight of ginseng extract, 0.001 to 15% by weight of licorice extract, 0.001 to 15% by weight of MSM (OptiMSM, Rohas Pia) 0.001 to 10% by weight of Sh-oligopeptide-2, 0.001 to 10% by weight and PDGF-A (Sh-polypeptide-58, Bio-FD & C) To prepare a composite composition.

At this time, the IGF-1 and PDGF-A were prepared in the form of liposome to improve the stability of the substance, and mixed with the remaining components to prepare a composite composition according to the present invention.

Example  2. Containing the composite composition of the present invention For hair  Manufacture of lotion

The hair lotion for hair containing the composite composition of Example 1-2 was prepared with the components and contents shown in Table 1 below. As shown in Table 1, the composition was prepared by mixing 0.0.5 wt% of carboxyvinyl polymer, 1.0 wt% of butylene glycol, 0.5 wt% of glycerin, 1.0 wt% of sodium di lauroylglutamate lysine, 0.307 wt% of the composite composition of Example 1-2, , And 0.5% by weight of PEG-4000 were heated in a purified water, mixed and dissolved to prepare an aqueous mixture. Thereafter, a solution obtained by dissolving polyoxyethylene hardened castor oil 0.2 wt%, polysorbate 80 0.2 wt%, triethanol amine 0.05 wt%, methyl paraben suitable amount, and perfume suitable amount in ethanol 18.0 wt% was mixed with the aqueous mixture to prepare a lotion .

As shown in the following Table 1, cosmetic lotions not containing lysine dilauroyl glutamate or the composite composition of Example 1-2 as Comparative Examples 1 to 3 were prepared in the same manner as in Example 2 above.

number division Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 One Carboxyvinyl polymer 0.05 0.05 0.05 0.05 2 Butylene glycol 1.0 1.0 1.0 1.0 3 glycerin 0.5 0.5 0.5 0.5 4 Sodium diureoylglutamate lysine 1.0 - - 1.0 5 PEG-4000 0.5 0.5 0.5 0.5 6 The composite composition of Example 1-2 0.307 - 0.307 - 7 Polysorbate-80 0.2 0.2 0.2 0.2 8 Polyoxyethylene hardened castor oil 0.2 0.2 0.2 0.2 9 ethanol 18.0 18.0 18.0 18.0 10 Methyl paraben Suitable amount Suitable amount Suitable amount Suitable amount 11 Triethanolamine 0.05 0.05 0.05 0.05 12 Spices Suitable amount Suitable amount Suitable amount Suitable amount 13 Purified water To 100 To 100 To 100 To 100

Example  3. Containing the composite composition of the present invention hair Nutritional fluid  Produce

Hair nutrition milky lotions containing the composite composition of Example 1-2 were prepared with the ingredients and contents shown in Table 2 below. As shown in Table 2, it was confirmed that 0.5 wt% of vegetable wax, 0.5 wt% of cetanol, 0.8 wt% of cetostearyl alcohol, 0.6 wt% of stearic acid, 0.6 wt% of self emulsification type monostearic acid, 0.5 wt% 0.5% by weight of paraben wax, 0.5% by weight of paraben wax and fragrance were separately mixed and heated and dissolved to prepare an alcohol mixture. Then, 0.1% by weight of the composite composition of Example 1-2 of the present invention, 1.0% by weight of sodium di lauroylglutamate lysine, 0.5% by weight of PEG4000, 4.00% by weight of concentrated glycerin, 6.00% by weight of butylene glycol, 0.08% %, Triethanolamine 0.08% by weight, an appropriate amount of preservative and the remaining balance of purified water were mixed and heated to dissolve. The alcohol mixture was added to the mixture, followed by stirring and cooling to prepare a hair nutrient solution.

As shown in the following Table 2, cosmetic lotions not containing lysine dilauroyl glutamate or the composite composition of Example 1-2 as Comparative Examples 4 to 6 were prepared in the same manner as in Example 3 above.

number division Example 2 Comparative Example 4 Comparative Example 5 Comparative Example 6 One Vegetable wax 0.5 0.5 0.5 0.5 2 Cetanol 0.5 0.5 0.5 0.5 3 Cetostearyl alcohol 0.8 0.8 0.8 0.8 4 Stearic acid 0.6 0.6 0.6 0.6 5 Self emulsifying monostearic acid 0.6 0.6 0.6 0.6 6 Chin type monostearate 0.5 0.5 0.5 0.5 7 Wax 0.5 0.5 0.5 0.5 8 Paraffin wax 0.5 0.5 0.5 0.5 9 Squalane 3.0 3.0 3.0 3.0 10 Polysorbate 60 1.2 1.2 1.2 1.2 11 Sesquioleic acid 0.8 0.8 0.8 0.8 12 Example 1-2 Composite composition 0.1 - - 0.1 13 Sodium diureuloylglutamate lysine 1.0 - 1.0 - 14 PEG 4000 0.5 0.5 0.5 0.5 15 Concentrated glycerin 4.0 4.0 4.0 4.0 16 Butylene glycol 6.0 6.0 6.0 6.0 17 Carboxy polymer 0.08 0.08 0.08 0.08 18 Triethanolamine 0.08 0.08 0.08 0.08 19 antiseptic Suitable amount Suitable amount Suitable amount Suitable amount 20 Spices Suitable amount Suitable amount Suitable amount Suitable amount 21 Purified water To 100 To 100 To 100 To 100

Example  4. Containing the composite composition of the present invention hair Of treatment  Produce

Hair treatments containing the composite composition of Example 1-2 were prepared with the ingredients and contents shown in Table 3 below. As shown in Table 3, it was found that when the beige wax was used in an amount of 1.00 wt%, cetanol 0.90 wt%, cetostearyl alcohol 1.00 wt%, stearic acid 0.80 wt%, self emulsification type monostearic acid 1.00 wt%, parent type monostearic acid 1.00 wt% 1.5% by weight of squalane, 0.50% by weight of paraffin wax, 3.00% by weight of capricaprylic triglyceride, 0.50% by weight of dimethicone, 1.20% by weight of polysorbate 60, 0.8% by weight of sorbitan stearate, Are separately mixed and heated to prepare an alcohol mixture. Then, the composite composition of Example 1-2 of the present invention containing 1.0% by weight of PEG4000, 0.5% by weight of PEG4000, 5.0% by weight of sodium di lauroylglutamate lysine, 6.0% by weight of concentrated glycerin, 6.0% by weight of butylene glycol, 0.1% by weight of triethanolamine, and 0.1% by weight of an antiseptic agent were mixed with a remaining amount of purified water and heated to dissolve. The alcohol mixture was added to the mixture and stirred to cool the mixture to prepare a hair treatment.

As shown in the following Table 3, cosmetic lotions not containing lysine dilauroyl glutamate or the composite composition of Example 1-2 as Comparative Examples 7 to 9 were prepared in the same manner as in Example 4 above.

number division Example 3 Comparative Example 7 Comparative Example 8 Comparative Example 9 One Vegetable wax 1.0 1.0 1.0 1.0 2 Cetanol 0.9 0.9 0.9 0.9 3 Cetostearyl alcohol 1.0 1.0 1.0 1.0 4 Stearic acid 0.8 0.8 0.8 0.8 5 Self emulsifying monostearic acid 1.0 1.0 1.0 1.0 6 Chin type monostearate 1.0 1.0 1.0 1.0 7 Wax 1.5 1.5 1.5 1.5 8 Paraffin wax 0.5 0.5 0.5 0.5 9 Squalane 6.0 6.0 6.0 6.0 10 Capric capric triglyceride 3.0 3.0 3.0 3.0 11 Dimethicone 0.5 0.5 0.5 0.5 12 Polysorbate 60 1.2 1.2 1.2 1.2 13 Sorbitan stearate 0.8 0.8 0.8 0.8 14 The composite composition of Example 1-2 0.1 - - 0.1 15 Sodium diureuloylglutamate lysine 1.0 - 1.0 - 16 PEG 4000 0.5 0.5 0.5 0.5 17 Concentrated glycerin 4.0 4.0 4.0 4.0 18 Butylene glycol 6.0 6.0 6.0 6.0 19 Carboxy polymer 0.08 0.08 0.08 0.08 20 Triethanolamine 0.08 0.08 0.08 0.08 21 antiseptic Suitable amount Suitable amount Suitable amount Suitable amount 22 Spices Suitable amount Suitable amount Suitable amount Suitable amount 23 Purified water To 100 To 100 To 100 To 100

Experimental Example  1. The present invention Example  Antioxidant Effect of Composite Composition of 1-2

The antioxidative effect of the composite composition prepared in Example 1-2 of the present invention was compared with the antioxidative effect of each of MSM, Sasa extract, Gossam extract and licorice extract prepared in Example 1-1. In order to confirm the antioxidant activity, SOD (Superoxide Dismutade-like Activity) was measured. SOD-like activity was measured by the method of Marklund et al. (Murklund, S. and Marklund, G.: Involvement of superoxide anion radicals in pyroalloy and a convenient assay for superoxide dismutase, Eur . J. Biochem . , 47, 468, 1974) .

The complex composition of Example 1-2 and Tris-HCl buffer (50 mM tris + 10 mM EDTA, pH (1)) were added to MSM as the sole preparation, 20 μl of the sample solution of each of the Sasa extract, 8.5) and 20 μl of 7.2 mM pyrogallol were reacted at 25 ° C. for 10 minutes. Then, 10 μl of 5N HCl was added to stop the reaction, and the amount of pyrogallol oxidized in the reaction solution was measured at 420 nm. MSM, MSM, and the extracts of Sasa extract, Gossam extract, and licorice extract prepared in Example 1-1 were repeated twice at a concentration of 100 ppm and 500 ppm, respectively, as the composite composition of Example 1-2.

The results of the experiment are shown in FIG. As shown in FIG. 1, the composite composition of Example 1-2 exhibited SOD-like activity of about 80% at a concentration of 100 ppm and SOD-like activity of at least 90% at a concentration of 500 ppm, SOD - like activity of sulfonyl methane (MSM), Sucrose extract, Gossam extract and Licorice extract was higher than that of SOD - like activity. It was confirmed that the composite composition of Example 1-2 of the present invention exhibited SOD-like activity twice as much as that of MSM exhibiting the highest SOD-like activity of about 40% in the single agent. Also, SOD-like activity is almost similar even when the concentration of the single agent is increased from 100 ppm to 500 ppm. However, in the case of the composite composition of Example 1-2 of the present invention, when the concentration is increased from 100 ppm to 500 ppm, 80% and 90%, respectively.

Experimental Example  2. The present invention Example  1-2 Antimicrobial Effect of Composite Composition

In order to confirm the effect of suppressing dandruff in the composite composition prepared in Example 1-2 of the present invention, an experiment comparing MSM, the dandruff inhibitory effect of each of the extracts of Sasa extract, Gossam extract and licorice extract prepared in Example 1-1 Respectively. The anti-dandruff effect was evaluated by an antibacterial test against maltose germ (Malassezia furfur ATCC 12078). The control group was inoculated and cultured without using any of the above test samples. The above Malassezia fungi were inoculated on SDA (Sabouraud Dextrose Agar) medium and inoculated. In the center of the plate medium, methylsulfonylmethane (MSM) as the single-agent preparation of the present invention and Example 1- The extracts of Sasa extract, Gossam extract and licorice extract prepared in 1 were applied and cultured at 30 ± 1 ℃ for 2 ~ 3 days.

W = (T - D) / 2

(W: width of low zone (nm), T: total diameter of sample and microbial low zone in nm, D: diameter of sample in nm)

The size of the microbial lowlands was calculated according to the above equation, and the experiment was performed three times each, and the results were averaged. The experimental results are shown in Table 4 below.

Test strain sample T (nm) D (nm) W (nm) Malassezia furfur
(ATCC 12078) Example 1-2 Composite composition 75 20 27.5 Methylsulfonylmethane (MSM) 40 20 10.0 Sashou extract 33 20 6.5 Gosam extract 26 20 3.0 Licorice extract 28 20 4.0 Control group 20 20 0.0

As shown in Table 4, the composite composition of Example 1-2 of the present invention and the MSM as the single agent, the mucilage extract, the ginseng extract and the licorice extract of Example 1-1 were formed into a support having a predetermined size, It was confirmed that it has antimicrobial activity against dandruce. Among them, it was confirmed that the composite composition of Example 1-2 of the present invention having the widest width of 27.5 mm in the lowlands has the highest antimicrobial activity against dandruff. It was confirmed that the composite composition of Example 1-2 of the present application was remarkably excellent in antimicrobial activity because it was about three times as large as the MSM having a low zone width of 10 mm in the single agent.

Experimental Example  3. The Example  Cell activity of the complex composition of 1-2

In order to confirm the cell activity effect of the composite composition prepared in Example 1-2 of the present invention, experiments comparing MSM and the cell activity of each of the extracts of Sasa extract, Gossam extract and licorice extract prepared in Example 1-1 Respectively.

Human fibroblast cells cultured in culture dishes were treated with 0.5% trypsin, and then 1 × 10 4 human fibroblasts were mixed with 100 μl of DMEM culture medium together with 10% fetal bovine serum, Were placed in each well of the plate. After incubation for 1 day at 37 ° C in a 5% CO 2 incubator, when the cells were completely attached to the bottom of a 96-well plate, the existing culture was removed and 1 μg / ml of the complex composition of Example 1-2 and methylsulfonylmethane (MSM), the extract of Sasa extract of Example 1-1, the extract of Gosam, and the licorice extract, respectively. As a control, the cells were treated with a DMEM culture solution in which each of the above samples was not added. Then, the cells were cultured in a 5% CO 2 incubator at 37 ° C for 48 hours. Then, the culture medium containing the respective samples was removed, and the remaining culture was completely removed from the 96-well plate using sterile phosphate buffered saline. Thiazolyl blue tetrazolium bromide (MTT) was dissolved in sterilized phosphate buffered saline at a concentration of 0.5 mg / ml. Each well was treated with 100 쨉 l of each well and then stored at 37 캜 for 3 hours. After completely removing the dissolved phosphate buffered physiological saline solution of MTT, 100 .mu.l of isopropanol was treated and allowed to stand at room temperature for 30 minutes, and the absorbance was measured at a wavelength of 540 nm. On the basis of the absorbance value of the cells of the control group, the composite composition of Example 1-2 of the present invention and the methylsulfonylmethane (MSM) as the sole preparation, the mucilage extract of Example 1-1, the ginseng extract and the licorice extract were treated The cell viability was evaluated by comparing the absorbance values of the cells.

The results of the experiment are shown in FIG. As shown in FIG. 2, the cell activity of the composite composition of Example 1-2 of the present invention was about 65%, and methylsulfonylmethane (MSM) as a single agent, the mucilage extract of Example 1-1, All of the licorice extracts exhibited cell activity of less than 5%, confirming that the composite composition of Example 1-2 of the present invention had remarkably superior cell activating effect as compared with the single preparations.

Experimental Example  4. The Example  1-2 < / RTI > Reductase  Inhibitory effect

In order to confirm the 5α-reductase inhibitory effect of the composite composition prepared in Example 1-2 of the present invention, the effect of 5α-reductase on the dihydrootestosterone (DHT) Experiments were performed: Schwartz JI, Tanaka WK. Steroid 5alpha-reductase inhibitors in androgen-depedent disorders. Curr Opin Chem Biol . 1997: 1 (2): 254-259.

Sprague-dawley rats were purchased from Hyochang Science (Daegu, Korea) to examine the effect of 5α-reductase on dihydrotestosterone (DHT), and testosterone, Nicotinamide, adenosine dinucleotide pohosphate (NADPH), sucrose, and Trizma base were purchased from Sihama Chemical Co., St. Louis, MO, USA.

The purchased Sprague Dawaur strain is a 7-week-old adult female. The liver was fasted for 24 hours, and the liver was washed three times with PBS (phosphate-buffered saline), and then placed in a 3 to 4-fold volume of ice cold buffer, 50 mM Na2HPO4, 0.25 M sucrose pH 7.2 , The suspension was prepared by pulverizing the hepatocytes with a homogenizer. The supernatant was obtained by subjecting the suspension to ultrasonic disruption for 5 minutes and then centrifugation at 900 X g for 10 minutes to isolate 5? -Reddactase from the suspension. The resulting supernatant was centrifuged at 9,000 x g for 10 minutes to obtain supernatant, and the enzyme solution was stored frozen at -80 ° C. All the above procedures were carried out at a low temperature of 0 to 4 ° C.

The 5α-reductase inhibitory activity was measured by modifying the method of Hartman (Hartamann et al.). The test substance is reacted with the 5α-reductase solution prepared above using testosterone as a substrate to quantitate metabolites. 1 mM of 10 M Tris-HCl buffer (pH 7,2) was mixed with 0.3 ml of the complex composition of Example 1-2 of the present invention and 4.5 mM of nicotinamide adenine dinucleotide phosphate (Sigma, C0, Mo, USA) Sigma, Co, Mo, USA), and the reaction conditions were incubated at 37 ° C for 30 minutes. The reaction was stopped by adding 5 ml of dichloromethane, followed by centrifugation at 3000 × g for 10 minutes. After the supernatant was removed, the dichloromethane layer was left and left in a hood for one day to remove the solvent. The residue was added with 3 ml of methanol, and the enzyme inhibition rate was calculated by the following formula (1) using high performance liquid chromatography . Shim-pack ODS (4.6 ㎛, 250 x 4.6 mm) was used as the column for the HPLC measurement, and methanol: water mixture (65:35) was used for the mobile phase. The flow rate was 1 ml / min. The detection wavelength was detected at UV 254 nm and the injection amount was 0.02 ml.

[Equation 1]

Inhibition rate (%) = (1 - amount of testosterone in the sample addition group / amount of testosterone in the no addition group) X 100

The above experiment was repeated several times with different concentrations of the composite composition of Example 1-2 of the present invention.

The results of this experiment are shown in FIG. As shown in FIG. 3, when the dihydrotestosterone (DHT) was used as a standard, the amount of testosterone decreased in the group treated with the composite composition of Example 1-2, 5-reductase inhibitory activity. Particularly, as the concentration of the composite composition of Example 1-2 was increased, the effect of the 5α-reductase inhibitory activity was increased, and in particular, the concentration of the composite composition exhibited a high 5α-reductase inhibitory activity of 1000 ppm to 39% there was.

Experimental Example  5. The Example  Anti-inflammatory effect of the complex composition of 1-2

In order to confirm the anti-inflammatory effect of the composite composition prepared in Example 1-2 of the present invention, MSM, an experiment for comparing the anti-inflammatory effect of each of the extracts of Sasa extract, Gossam extract and licorice extract prepared in Example 1-1 Respectively.

The anti-inflammatory effect was measured as an inhibitory activity against nitric oxide (Nitric Oxide). Lipopolysaccharide (LPS) was used as an inflammatory stimulus source, and experiments were conducted using distilled water as a control group.

The amount of nitric oxide produced from macrophages (Raw 264.7 cells) was determined by the method of Byun, SH, Yang, CH and Kim, SC: Inhibitory effect of Scrophulariae radix extract on TNF-a, IL-1β, Nitric Oxide production in lipoplysaccharide-activated mouse macrophage Raw 264.7 cells. Kor J. Ori . Herbology . , 20, 7-16, 2005). The amount of NO2- present in the cell culture medium was determined using a standard curve of NaNO2.

The results of the experiment are shown in FIGS. 4 and 5.

As shown in FIGS. 4 and 5, methylsulfonylmethane (MSM) which is a single agent, mossy extract of Example 1-1, gossam extract, licorice extract, as well as the composite composition of Example 1-2 of the present invention, And anti-inflammatory effect on LPS. In particular, the composite composition of Example 1-2 showed an amount of nitric oxide of 0.05 (μg / ml) or less, which was about twice as much as the amount of nitric oxide of about 0.1 (μg / ml) And the effect is shown.

Experimental Example  6. The Example  ≪ RTI ID = 0.0 > Cosmetics  Measurement of moisture content of composition

The moisture resistance of Examples 2 to 4 and Comparative Examples 1 to 9 prepared using the composite composition of Example 1-2 was measured.

The moisture-improving effect of the above Examples 2 to 4 and Comparative Examples 1 to 9 was evaluated through a moisture device (Corneometer CM825, Courage + Khazaka) capable of measuring moisture content in the skin as the electric conductivity. Examples 2 to 4 are the cosmetic compositions of various formulations including the composite composition and the moisturizing agent of Example 1-2 of the present invention. Comparative Example 1 to Comparative Example 3 are the same as Example 2 except for the inclusion of the composite composition or moisturizing agent (lysine sodium sodium dilaurylglutaminate) of Example 1-2. In Comparative Examples 4 to 6, the other components were the same as those in Example 3, except that the composite composition or humectant of Example 1-2 was included. Comparative Example 7 to Comparative Example 9 are the same as Example 4 except that the composite composition or humectant of Example 1-2 is included.

As a test method, 60 healthy women from 35 to 55 years old were used for 5 months in each of the examples and the comparative example once a day for 2 months. Prior to the start of the experiment, the moisture content in the scalp was measured using the moisture device at the scalp site, and the moisture content in the scalp was measured at the same site of the scalp immediately after the completion of the experiment using the moisture device. The moisture content was measured using an average value measured five times in each portion. The measurement results are shown in Table 5 below.

division Moisture power division Moisture power Degree of moisturization increase Comparative Example 1 10.79 Example 2 12.35 1.56 Comparative Example 2 11.29 0.50 Comparative Example 3 11.35 0.56 Comparative Example 4 19.30 Example 3 21.78 2.48 Comparative Example 5 20.27 0.97 Comparative Example 6 20.44 1.14 Comparative Example 7 25.74 Example 4 27.47 1.73 Comparative Example 8 25.82 0.08 Comparative Example 9 26.73 0.99

Comparative Example 1, Comparative Example 4, and Comparative Example 7 were the same as those of Examples 2 and 3 (Comparative Examples 1 and 2) except that both of the moisturizing agent (sodium diureoylglutamate lysine) and the composite composition of Example 1-2 were not used, , 4 shows the increase / decrease by use of both the humectant (sodium diureoylglutamate lysine) and the composite composition of Example 1-2, and Comparative Examples 2, 3, 5, 6, 8, and 9 .

As shown in Table 5, the moisture increase of each of Examples 2 to 4 was 1.56, 2.48, and 1.73, respectively, and the sum of moisture increase of Comparative Examples 2 and 3, that of Comparative Example 5 and Comparative Example 6 And the increase in moisture resistance of Comparative Example 8 and Comparative Example 9, respectively. Therefore, in terms of increasing the moisturizing power, the preparations of Examples 2 to 4 of the present invention have a synergistic effect compared with the case of using the humectant (sodium diureoylglutamate lysine) or the composite composition of Example 1-2 .

Experimental Example  7. The Example  1-2 < / RTI > Cosmetics  Skin stability test of composition

In order to perform the skin stability test, 90 patients (mean age 25 years, age range 20 to 36 years) were subjected to a skin patch test on the upper arm using Haye's Test Chamber, Respectively. However, those who are taking psoriasis, eczema, other skin lesions, pregnancy, breastfeeding or contraceptive, and antihistamines are excluded from this experiment.

The test site was wiped with 70% ethanol and then dried. Then 0.5 g of each of the cosmetic compositions prepared in Example 2-4 and Comparative Examples 2, 3, 5, 6, 8 and 9 was placed on the upper part of the test area and fixed . After applying the patch for 24 hours and removing the patch, the test area was marked with a pen, and the test site was observed after 30 minutes, 24 hours, and 48 hours, respectively.

The judgment was made 30 minutes, 24 hours, 48 hours after removal of the patch, and skin reactions were judged according to the criteria of the International Contact Dermatology Research Group (ICDRG) listed in Table 6 below.

sign Criteria evaluation Mean Score ± Doubtful or slight reaction and erythema Small stimulus 0 to 0.9 + Erythema + Induration Light stimulus 1.0 2.9 ++ Erythema + Induration + Vesicle Moderate irritation 3.0 ~ 4.9 +++ Erythema + Induration + Bullae River stimulation 5.0 or higher - Negative No stimulation 0

The results of the experiment are shown in Table 7 below, and the degree of stimulation was calculated as shown in Equation 2 below.

&Quot; (2) "

Number of subjects / number of test subjects = {[(±) number X 1] + [(+) number X 2] + [(++) number X 3]

division Test embroidery Judgment result Irritation degree ++ + ± - Example 2 10 0 One 3 6 0.5 Comparative Example 2 10 0 One 4 5 0.6 Comparative Example 3 10 0 2 4 4 0.8 Example 3 10 0 One 2 7 0.4 Comparative Example 5 10 0 One 3 6 0.5 Comparative Example 6 10 0 One 3 6 0.5 Example 4 10 0 One 4 5 0.6 Comparative Example 8 10 0 4 4 5 0.6 Comparative Example 9 10 0 5 5 4 0.7

As shown in Table 7, Examples 2 to 4 of the present invention are more safe than the cosmetic compositions prepared in Comparative Examples 2, 3, 5, 6, 8 and 9, All were found to be significantly safer to the skin.

Experimental Example  8. The Example  Increase of hair thickness of composite composition 1-2

The hair thickness increase experiments were performed using the cosmetic compositions of Examples 2 and 3 prepared by incorporating the composite composition of Example 1-2 of the present invention.

The selection of the subjects was made by 20 men and women who showed different symptoms of hair loss in different environments and working conditions. Whether or not the hair loss symptom was selected was determined based on the number of times the hair was dropped during the hair washing. Experiments were conducted for those who had more than 100 hair removed after hair washing. The age of these subjects was 30 to 60 years old, considering the need for hair care, and the average age was 42.7 years.

The cosmetic compositions prepared according to Examples 2 and 3, Comparative Example 1 and Comparative Example 4 were applied to 20 subjects for 4 months, and hair thickness was measured every month. The thickness of the hair was measured before and after use of A and B in 1 cm < 2 > of hair loss region, and the hair was taken after 4 months of use. The thickness of the hair was measured with an electron microscope and the average values were compared. .

Hair thickness (㎛) Subject 1 Subject 2 Subject 3 Subject 4 Subject 5 A B A B A B A B A B Example 2 Before use 44 46 50 53 54 52 54 51 51 52 4 months 85 88 92 90 89 85 80 85 93 91 Example 3 Before use 46 48 40 42 47 47 46 45 43 44 4 months 87 89 83 84 83 82 85 87 82 84 Comparative Example 1 Before use 48 47 48 48 49 47 50 51 59 50 4 months 48 48 49 48 48 47 51 53 58 52 Comparative Example 4 Before use 47 47 46 48 41 43 41 40 45 47 4 months 47 48 47 47 42 43 43 41 44 46

As shown in Table 8, Comparative Example 1 and Comparative Example 4 showed no significant change in the thickness of the hair before or after 4 months, while the composite composition of Example 2 and Example 3 of the present invention was prepared When a cosmetic composition is used, it can be confirmed that the thickness of the hair after 4 months is thickened by about 2 times as compared with that before use. Therefore, the cosmetic composition containing the composite composition according to the present invention has an effect of thickening the hair .

Experimental Example  9. The Example  1-2 Composite Composition Dropout hair  Reduction experiment

Sample Nos. 2 and 3 used in Experimental Example 8; Using Comparative Example 1 and Comparative Example 4 as a sample, a fall-off hair reduction experiment was conducted. The experiment was divided into four groups of 20 men and women with hair loss symptom. After the daily shampooing, the samples were lightly patched as if massage and then washed with water. Then, the number of missing hair And the number of selectors according to the number of dropped hair is shown in Table 9 below.

Number of missing hair Number (persons) 100 to 150 81 ~ 99 61 to 80 30 to 60 Example 1 1 month use One One 2 One 4 months use - - 2 3 Example 2 1 month use - 2 2 One- 4 months use - One 3 One Comparative Example 1 1 month use 3 2 - - 4 months use 3 One One - Comparative Example 4 1 month use 4 One - - 4 months use 3 2 - -

As shown in Table 9, when the cosmetic composition (Examples 2 and 3) prepared using the composite composition of Example 1-2 of the present application was used as a massage after washing the hair, it was applied for 1 month to 4 months It was confirmed that the number of hair falling off was remarkably decreased. On the other hand, the compositions of Comparative Example 1 and Comparative Example 4 showed no effect on the reduction of dropout hair even after 1 month or 4 months of use.

Although several embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various modifications may be made without departing from the principles and spirit of the invention . The scope of the invention will be determined by the appended claims and their equivalents.

Claims (10)

Hair growth inhibition and hair growth inhibitory action comprising an effective ingredient of a complex composition comprising a mushroom extract, a ginseng extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF), and a methylsufonyl methane A cosmetic composition for promotion. A composition for preventing dandruff formation, which comprises as an active ingredient, a complex composition comprising a mushroom extract, a ginseng extract, a ginseng extract, a licorice extract, an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF), and a methylsufonyl methane Cosmetic composition. (1), a complex composition comprising an insulin like growth factor-1 (IGF-1), a platelet derived growth factor (PDGF) and a methylsufonyl methane (MSM) Cosmetic composition. 4. The method according to any one of claims 1 to 3,
0.001 to 20% by weight, respectively, of the Sucrose extract, the Gossam extract, the licorice extract and the MSM, relative to the composite composition; Wherein the IGF-1 and the PDGF each comprise 0.001 to 15 wt%. 5. The method of claim 4,
Wherein the extract is an extract which is soluble in water containing purified water, a lower alcohol having 1 to 4 carbon atoms, a nonpolar solvent or a mixed solvent thereof. 6. The method of claim 5,
Wherein the composite composition comprises 0.0001 to 10% by weight based on the total weight of the cosmetic composition. 6. The method of claim 5,
Further comprising a moisturizing agent,
Wherein the composite composition is contained in an amount of 0.01 to 0.5 times the amount of the moisturizing agent added. 8. The method of claim 7,
Wherein the moisturizing agent is 0.01 to 20% by weight and the composite composition is 0.001 to 10% by weight based on the total weight of the cosmetic composition. 9. The method of claim 8,
Wherein the humectant is dilauroyl glutamate lysine or a non-toxic salt thereof. 10. The method according to claim 6 or 9,
In the cosmetic composition,
Hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massager cream, hair wax, hair aerosol, hair pack, hair Hair dyes, hair lacquers, hair moisturizers, hair mousse or hair, hair dyes, hair dyes, hair gels, hair dressers, hair lacquers, hair moisturizers, hair moisturizers, A cosmetic composition which is a formulation of spray.

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