KR101797072B1 - Composition for preventing, treating, or improving ocular fatigue, ocular inflammation, or dry eye syndrome comprising Camellia japonica extracts - Google Patents
Composition for preventing, treating, or improving ocular fatigue, ocular inflammation, or dry eye syndrome comprising Camellia japonica extracts Download PDFInfo
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- KR101797072B1 KR101797072B1 KR1020150176179A KR20150176179A KR101797072B1 KR 101797072 B1 KR101797072 B1 KR 101797072B1 KR 1020150176179 A KR1020150176179 A KR 1020150176179A KR 20150176179 A KR20150176179 A KR 20150176179A KR 101797072 B1 KR101797072 B1 KR 101797072B1
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- Prior art keywords
- eye
- composition
- camellia
- present
- dry eye
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Abstract
The present invention relates to a composition for preventing, treating or ameliorating eye fatigue, eye inflammation, or dry eye syndrome, which comprises Camellia japonica extract as an active ingredient. The camellia extract, which is an effective ingredient of the present invention, In animal models, it promotes tear production, delays tear film breakdown time, slows corneal epithelial damage, decreases inflammatory cytokine expression on the ocular surface and lacrimal gland, and increases macrophage infiltration To prevent, treat, or ameliorate eye fatigue, eye inflammation, or dry eye syndrome. In addition, the camellia extract of the present invention has no cytotoxicity and side effects, and can be safely used in a pharmaceutical composition, a quasi-drug composition, or a food composition.
Description
The present invention relates to a method for preventing, treating or ameliorating eye fatigue, eye inflammation, or dry eye syndrome, which comprises a camellia extract as an active ingredient. More specifically, the present invention relates to a method for preventing, treating or ameliorating eye fatigue, Quasi-drugs or food compositions for the prevention, treatment, or amelioration of inflammation of the eye, dryness of the eye, and the like.
In modern society, the boundary between night and day is vague, and the artificial light that exists around us has brought out the darkness brought about by the night, which has brought remarkable development and advantages in many ways. On the contrary, it was accompanied by a side effect of the eye.
In recent years, an increase in the number of people complaining of decreased visual acuity, eye fatigue, tinnitus, hearing loss, irritable colitis, psoriasis, atopy, and accompanying headache symptoms is one of the side effects of eyes. In addition, And eye fatigue caused by the use of computers is becoming more and more serious. In particular, the use of smartphones, the development of computers, and the prolonged use of eyes due to the excessive fatigue of examinees are causing the eyes to become easily tired, the lack of water in the tear film and the increase of the inflammatory reaction, which is the biggest cause of dry eye syndrome have. Therefore, materials capable of preventing or improving dry eye syndrome as well as relieving eye fatigue and inflammatory reaction have been studied and developed. Recently, misuse and abuse of drugs and adverse reactions to chemicals have become serious problems, There is a need for a new replacement drug that is derived.
On the other hand, Camellia japonica is a bamboo evergreen tree belonging to the white tree and camellia. In Korea, it is distributed to Daechung in the southern coastal area and west, and Ulleungdo in the east. In Jeonnam region, 67% of the total area of the whole country is occupied. Camellia seeds have been used as edible oil and cosmetics, stem has been used as a raw material for high quality charcoal, and leaves have been used as tea materials in tea-free areas. As a pharmacological effect, camellia leaves have been used for psoriasis, sore throat and burn. Branches and fruits have been used for hair dandruff, blood, hemorrhage, eosinophilia, cartilage abnormality, diuretic, Alcohol (Triterpene alcohol) has been reported to effectively alleviate inflammation of the ear induced by tPA (tissue plasminogen activator). However, little is known about the role of camellia in eye diseases such as eye fatigue, eye irritation, dry eye syndrome, etc., and there has been no report on the results of applying camellia extract to eyes for ophthalmic treatment purposes.
Accordingly, the present inventors not only relieved eye fatigue and inflammation, but also developed new substances for preventing or treating dry eye syndrome. Camellia extract promoted tear production in an eye-dry animal model, delayed tear film breakdown time , Which not only delays corneal epithelial damage but also reduces inflammatory cytokines in the ocular surface and lacrimal gland and inhibits infiltration of macrophages by inflammation to prevent eye fatigue, eye inflammation, or dry eye syndrome Or improvement of the present invention, thereby completing the present invention.
One object of the present invention is to provide a composition for preventing, treating or ameliorating eye fatigue, eye inflammation, or dry eye syndrome, which comprises a camellia extract as an active ingredient.
It is another object of the present invention to provide a pharmaceutical composition, a quasi-drug composition or a food composition for preventing, treating or ameliorating eye fatigue, eye inflammation, or dry eye syndrome.
The present invention provides a composition for preventing, treating or ameliorating eye fatigue, eye inflammation, or dry eye syndrome comprising Camellia japonica extract as an active ingredient.
In the present invention, the camellia extract is obtained by drying and pulverizing at least one selected from the group consisting of the stem, branch, leaf, fruit, bark and root of a camellia, preferably a camellia stem, branch or leaf Next, extraction can be carried out by an extraction method known in the art, for example, a direct extraction method using an extraction solvent, a steam distillation method, a supercritical fluid extraction method, or a cold pressing method. In one specific embodiment of the present invention, supercritical fluid extraction was used.
When the camphor extract is extracted using an extraction solvent, the extraction solvent may be, for example, water, anhydrous or lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, n-propanol, iso- A polar solvent comprising normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol), acetic acid, dimethylformamide (DMFO) or dimethyl sulfoxide (DMSO); Or acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, Butene, 1-chloropentane, o -xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethylsulfide, chloroform, dichloromethane, - Non-polar solvents including dichloroethane, anniline, diethylamine, ether, carbon tetrachloride and THF may be used.
In addition, the above-mentioned camellia extract can also be obtained through a conventional purification process in addition to the extraction using the above-mentioned extraction solvent. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The camphor extract can also be obtained through fractionation.
In the present invention, the camellia extract may be any extract, fraction and purified product obtained in each step of extraction or purification (separation, fractionation), diluted solution thereof, concentrate or dried product thereof.
In the present invention, the camellia extract obtained by the above-mentioned method promotes tear production, delays tear film breakdown time, delays corneal epithelial cell damage, as well as inflammation of the eye surface and lacrimal gland, Decrease the expression level of cyne and inhibit macrophage infiltration by inflammatory reaction.
Therefore, the composition containing the camellia extract of the present invention as an active ingredient can be usefully used for preventing, treating or improving eye fatigue, eye inflammation, or dry eye syndrome.
Since the camellia extract contained as an active ingredient in the composition of the present invention is extracted from camellia which is a natural plant material, there is no adverse effect on human body even when it is administered in an excessive amount. Therefore, the quantitative upper limit of the amount of camellia extract contained in the composition of the present invention is .
In a preferred embodiment of the present invention, the camellia extract contained as an active ingredient in the pharmaceutical composition of the present invention may be contained in an amount of 0.01 to 10% by weight, preferably 0.05 to 5% by weight, based on the total weight of the whole composition have.
As used herein, the term "comprising as an active ingredient" is meant to include an amount sufficient to effect the efficacy or activity of the camellia extract described above.
The composition of the present invention may contain, in addition to the above-mentioned camellia extract, an effective ingredient such as an antioxidant, a steroid-based or non-steroidal anti-inflammatory drug, etc. useful for prevention, treatment or improvement of eye fatigue, eye inflammation, .
As the antioxidant, ascorbic acid and its ester, sodium bisulfite, butylated hydroxytoluene, butylated hydroxyanisole, tocopherol, or a combination thereof may be used, but the present invention is not limited thereto.
Examples of the anti-inflammatory drug include, but are not limited to, dexamethasone, fluorometholone, prednisolone, bromfenac, diclofenac, prubiprofen, ketorolac, or a salt thereof.
As one specific use of the present invention, the composition of the present invention can be used as a pharmaceutical composition for preventing or treating eye fatigue, eye inflammation, or dry eye syndrome.
When the composition of the present invention is used as a pharmaceutical composition, in addition to the camellia extract as an active ingredient, a buffer, a viscosity regulator, a surfactant, a stabilizer, a stabilizer, A chelating agent, and a combination thereof, or a pharmaceutically acceptable carrier.
Examples of the buffer include MES (2- (N-morpholino) ethanesulfonic acid hemin sodium salt), HEPES (N- {hydroxyethyl} piperazine-N '- {2- ethanesulfonic acid} -Bis (2-hydroxyethyl) 2-aminoethanesulfonic acid), MOPS (3- {N-morpholino} propanesulfonic acid), BIS- ) Methane), citrate buffers, maleate buffers, succinate buffers, malate buffers, boric acid / sodium borate buffers, or combinations thereof may be used, but are not limited thereto.
As the viscosity adjusting agent, polyvinyl alcohol, polyvinyl pyrrolidone, methyl cellulose, hydroxypropylmethyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, or a combination thereof may be used, It is not.
Examples of the surfactant include polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol, ethylene glycol, propylene glycol, polysorbate such as Tween 80, Tween 60, Tween 20, poloxamer such as Pluronic F127, Pluronic F108 ), Poloxamines (e.g., Tetronic 1508, Tetronic 908), Brij, Myrj, long chain fatty alcohols having a carbon number of 12 or more (e.g., oleyl alcohol, stearyl alcohol, myristyl alcohol and docosohexanoyl Alcohol, etc.) or a combination thereof may be used, but is not limited thereto.
The pharmaceutically acceptable carriers are those conventionally used in the art to which the present invention belongs and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate , Microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, no. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).
The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably administered by parenteral administration, more preferably by intravenous infusion, subcutaneous injection, muscle injection, intraperitoneal injection, percutaneous administration, mucosal administration, Administration, and more preferably, topical administration.
A suitable dosage of the pharmaceutical composition of the present invention may vary depending on such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition of the present invention is 0.01 to 80 mg / kg (body weight) on an adult basis, preferably 0.1 to 60 mg / kg (body weight). In addition, depending on the judgment of a doctor or a pharmacist, it may be administered once or several times a day at intervals of a certain time. In particular, in the case of topical administration, it is preferable that the preparation is not used once to several times per day in an amount of 0.001 to 3% (w / v, the same applies), preferably 0.01 to 1%.
The pharmaceutical composition of the present invention may be formulated into a unit dosage form by using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container.
According to a preferred embodiment of the present invention, the formulation of the pharmaceutical composition of the present invention may be formulated as a solution, suspension, syrup, emulsion, liposome, excipient, powder, granule, tablet, ointment, An eye drop, a contact lens cleaner or a contact lens lubricant, and may further comprise a dispersant or stabilizer.
As another specific use of the present invention, the composition of the present invention can be used as a quasi-drug composition for preventing or ameliorating eye fatigue, eye inflammation, or dry eye syndrome.
When the composition of the present invention is used as a quasi-drug composition, it may be suitably used according to a conventional method such as using the camellia extract as it is or using it with other quasi-drugs or quasi-drugs.
The quasi-drug composition may be prepared and used in the form of a product selected from the group consisting of a sterilizing agent, a shower foam, an eye wash solution, a wet tissue, a hand wash, a humidifier cleaning agent, an ointment agent and a filter cleaning agent, .
As another specific use of the present invention, the composition of the present invention can be used as a food composition for preventing or improving eye fatigue, eye inflammation, or dry eye syndrome.
When the composition of the present invention is used as a food composition, the camellia extract may be directly added or may contain other food or food ingredients. For example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Daisaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavors (saccharine, aspartame, etc.) can be used as flavorings.
There is no particular limitation on the kind of the food. Examples of the food to which the camellia extract can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums and ice cream, various soups, drinks, tea, , Alcoholic beverages and vitamin complexes, and includes all health foods in a conventional sense.
When the food composition of the present invention is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and the like may be further added in addition to the camellia extract of the present invention.
In addition, the food composition may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin , Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the food composition of the present invention may contain flesh for the production of natural fruit juices, beverages and vegetable drinks. These components may be used independently or in combination.
On the other hand, the camellia extract of the present invention is extracted from camellia, which is a natural material, and is harmless to the human body. Since it has little toxicity and side effects, it can be safely used for long-term use. It can be safely applied.
The camellia extract used as an active ingredient in the present invention promotes tear production, delays tear film breakdown time, delays corneal epithelial cell damage, as well as the expression of inflammatory cytokines in the ocular surface and lacrimal gland, Treatment or amelioration of ocular fatigue, inflammation of the eye, or dryness of the eye by inhibiting the infiltration of macrophages by an inflammatory reaction. In addition, the camellia extract of the present invention has no cytotoxicity and side effects, and can be safely used in a pharmaceutical composition, a quasi-drug composition, or a food composition.
FIG. 1 is a graph showing the tear production promoting effect on the dry eye animal model (DED) of the camellia extract.
2 is a graph showing the effect of the camellia extract on the ocular dry animal model (DED) of inhibiting tear film destruction.
FIG. 3 is a graph showing the inhibitory effect of camellia extract on corneal epithelial cell damage to an animal model of dry eye (DED).
FIG. 4 is a graph showing the effect of inhibiting macrophage infiltration on an animal model of dry eye (DED) of camellia extract. FIG.
FIG. 5 is a graph showing the effect of inhibiting the secretion of inflammatory cytokines on the ocular dry animal model (DED) of camellia extract.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .
Example 1: Preparation of camellia extract
The camellia wood was dried by cold drying, and the dried camellia tree was crushed to a particle size of 0.2 mm to 2 mm to prepare a camellia powder. Carbon dioxide, which is an extraction solvent, was prepared by liquefaction by injecting into a cooler at -20 ° C.
Next, the camellia seed powder is firstly extracted with supercritical carbon dioxide at a temperature of 40 to 50 ° C and a pressure of 100 to 200 bar, and then the first supercritical extract of the camellia is extracted at a temperature of 45 to 55 ° C and a pressure of 100 to 200 bar Extracted with supercritical carbon dioxide under pressure, essential oils containing a large amount of functional ingredients such as triterpene, tocopherol and flavonoid were extracted from camellia tree with high efficiency.
Example 2: prevention or treatment of dry eye syndrome using camellia extract Experiment on effects
2-1. Materials and methods
Experimental dry eye (EDE) and experimental procedure
8-week-old C57BL / 6 mice were exposed to 0.5 mg / 0.2 ml scopolamine hydrobromide (Sigma-Aldrich, St. Louis, MO) three times a day Hour, 13: 30, and 18: 00) to induce dry eye. At this time, the experimental space was maintained at a temperature of 30% humidity and a temperature of 25 ° C.
During the experiment, animal behavior, food and water intake were not restricted. Mice were distributed into the following six groups: untreated (UT); EDE control group (eye drop group); Balanced salt solution (BSS, Alcon, Forth value, TX); 0.001% camellia extract treated group; 0.01% camellia extract treated group; 0.1% camellia extract treated group. At this time, the eye drops containing the camellia extract were prepared by diluting with BSS.
Then the mice that induced dry eye syndrome were treated with eye drops or balanced salt containing 2 μl of camellia extract four times a day.
Analysis of tear production
(Phenol-red impregnated cotton thread, Zone-quick, Oasis, Glendora, CA) was applied to the external conjunctival sac of the mouse treated with eye drops containing camellia extract for 20 seconds Lt; / RTI > The length of the cotton yarn turned red by tears was then measured using a microscope (SMZ 1500; Nikon, Melville, NY) and then converted to volume by substitution with the standard curve.
Tear film breakdown time analysis
In order to examine the tear film break-up time, which is a factor for determining tear film stability, which is the most important clinical factor for dry eye syndrome, a conjunctival sac of mouse treated with eye drops containing camellia extract after induction of dry eye syndrome , 1 μl of 1% Fluorescein was instilled, eyes were closed about 3 times, and the eyes were opened and gently spread. Then, a slit tear film was prepared through a slit lamp microscope equipped with a Cobalt blue filter The time (in seconds) until the first defect occurred was measured.
Corneal surface damage analysis
After induction of dry eye syndrome, 1 μl of 1% Fluorescein was instilled into the conjunctival sac of mice treated with eye drops containing camellia extract, and the mice were washed with saline and then the rat cornea was observed with a slit- The degree of damage (degree of fluorescent staining) was scored and evaluated. For this purpose, the cornea was divided into quarters, and each quadrant was evaluated to 0-4 points as follows (total 0-16 points).
Analysis of inflammation-mediated cytokine concentration
After the induction of dry eye syndrome, the conjunctiva of mice treated with eye drops containing camellia extract was collected through surgery. Quantitation of cytokines known to cause inflammation by Western blot analysis after protein separation was quantified.
Specifically, after the induction of dry eye syndrome, conjunctival tissues of mice treated with eye drops containing camphor extract were washed twice with PBS solution, and then lysis buffer (PRO-PREP protein extraction solution, Intron Biotechnology, Korea) Lt; 0 > C for 10 minutes. The cells were then centrifuged at 17,000 rpm, 4 ° C for 20 minutes, and the supernatant was recovered. Then 30 μg of each supernatant was mixed with SDS loading buffer (60 mM Tris, 25% glycerol, 2% SDS, 0.5% 2-mercaptoethanol, 0.1% bromophenol blue) Was loaded onto a polyacrylamide gel, electrophoresed and transferred to a nitrocellulose membrane (NCM). Then, the NCM was blocked with TBS (10 mM Tris, 100 mM NaCl, 0.1% tween 20) solution containing 5% unmodified milk powder at room temperature for 1 hour. TTBS solution for 10 minutes, followed by reaction for 2 hours with primary antibody (Prx2, Prx4, Prx5 diluted 1: 1000 each) and then washed three times with TTBS solution. Then peroxidase (peroxidase) contains a secondary antibody (goat-anti mouse 1: 10,000 , goat-anti rabbit 1: 10,000) sikyeotgo reaction for 1 hour, the detection of the antibody Amersham ECL TM Western Blotting Detection Reagents ( GE Healthcare, UK) and confirmed by LAS (GE Healthcare, UK).
Immunohistological analysis
After induction of dry eye syndrome, eye tissues containing camellia extract were fixed in 10% formalin, embedded in paraffin, and fixed on a slide glass with a thickness of 4 ㎛. The slide glass was then immersed in ethanol and blocked with BSA. The anti-CD11b antibody was then treated at room temperature for 3 hours. After washing with PBS, the FITC-conjugated anti-goto secondary antibody was treated and mounted on a mounting solution containing DAPI. The BX-40 apparatus equipped with eXcope, X3 digital camera (DIXI Optics, Korea) (Olympus, Japan).
Statistical analysis
The results were expressed as standard error (± SEM). The statistical significance of the differences between the experimental groups was analyzed by one-way analysis of variance (ANOVA), Turkish post test, SPSS 17.0 software (SPSS Inc., Chicago, ). P values less than 0.05 were considered statistically significant.
2-2. Experiment result
Tear production and tear film breakdown analysis
After treatment of the eye drops containing the camellia extract, mice treated with dry eye syndrome were treated with the method described above to analyze the effect of restoring the tear secretion and blocking the tear film breakdown time. As a result, as shown in FIGS. 1 and 2, the 0.1% camellia extract was found to promote secretion of tears reduced by dry eye syndrome, and it was shown to inhibit the tear film destruction by dry eye syndrome.
Corneal epithelial cells Degree of damage analysis
The corneal epithelial cell damage was measured by the method described above after the eye drop containing the camellia extract was treated with the dry eye syndrome-induced mouse, and the cornea was stained with fluorescent dye. As a result, as shown in FIG. 3, it was confirmed that the 0.01% and 0.1% camellia extract effectively inhibited corneal epithelial cell damage by dry eye syndrome.
Immunohistological analysis
After treatment with eye drops containing Camellia sinensis extract, the mouse models that induced dry eye syndrome were examined to determine whether they inhibited macrophage infiltration by inflammation in the cornea. As a result, as shown in FIG. 4, it was confirmed that the 0.1% camellia extract effectively inhibited macrophage infiltration induced by dry eye syndrome.
Analysis of inflammatory mediated cytokine expression
After treatment of eye drops containing camellia extracts in a mouse model that induced dry eye syndrome, the amount of inflammatory cytokine protein expression in the conjunctival tissues was measured in order to confirm whether or not inflammatory reaction-induced increase in inflammatory cytokine could be suppressed in the conjunctival tissue . As a result, as shown in FIG. 5, it was confirmed that 0.001%, 0.01% and 0.1% of camellia extract effectively reduced the expression level of inflammatory cytokines induced by dry eye syndrome.
Claims (4)
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| KR1020150176179A KR101797072B1 (en) | 2015-12-10 | 2015-12-10 | Composition for preventing, treating, or improving ocular fatigue, ocular inflammation, or dry eye syndrome comprising Camellia japonica extracts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20210065576A (en) | 2019-11-27 | 2021-06-04 | 전라남도 | Composition for anticancer or cancer supplement comprising extracts of raw pericarp derived from immature fruit of Camellia japonica |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301400A (en) * | 2008-06-18 | 2008-11-12 | 沈阳药科大学 | Camellia functional instant formulation and preparation thereof |
| JP2009155317A (en) | 2007-12-27 | 2009-07-16 | Bhn Kk | Anti-inflammatory agent and skin external medicine |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009155317A (en) | 2007-12-27 | 2009-07-16 | Bhn Kk | Anti-inflammatory agent and skin external medicine |
| CN101301400A (en) * | 2008-06-18 | 2008-11-12 | 沈阳药科大学 | Camellia functional instant formulation and preparation thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210065576A (en) | 2019-11-27 | 2021-06-04 | 전라남도 | Composition for anticancer or cancer supplement comprising extracts of raw pericarp derived from immature fruit of Camellia japonica |
| KR102297152B1 (en) | 2019-11-27 | 2021-09-02 | 전라남도 | Composition for anticancer or cancer supplement comprising extracts of raw pericarp derived from immature fruit of Camellia japonica |
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| KR20170069357A (en) | 2017-06-21 |
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