JPS5916816A - Composition for external use - Google Patents

Abstract

PURPOSE:A composition for external use, containing a fractionated substance obtained by treating a tree sap of an aloe with activated carbon by the batch process and if desired heat-treating and/or extracting the tree sap with an organic solvent and vitamin E, and having improved cure promoting action on wounds. CONSTITUTION:A composition for external use containing vitamin E of a substance containing the vitamin E and a fractionated substance obtained by treating a tree sap of an aloe by the batch process, preferably with 1-10wt%, based on the tree sap of the aloe, plant coal, e.g. coconut husk coal of 100-400 mesh, and if desired heat-treating the tree sap at 60-80 deg.C under ordinary or reduced pressure and/or extracting the tree sap with a water-soluble organic solvent to collect the extract. The range of amounts of the both components based on the total compositon are preferably as follows: 0.001-10wt% vitamin E and 0.01- 5wt% fractionated substance of the aloe.

Description

[Detailed description of the invention] Regarding the composition.

Conventionally, vitamin E has been used in cosmetics and other fields to increase peripheral blood volume, promote sebum secretion, and soften and moisturize skin. However, it is difficult to say that cosmetics containing vitamin E have been sufficiently effective.

In addition, aloe vera and aloe extract have traditionally been widely used, especially in folk medicine, to treat cracks, nicks, burns, cuts, etc., and it is also known that aloe extract is included in cosmetics, but aloe extract is unique to aloe vera. It has a bitter smell and taste,
1. In addition to being dark brown in color, it also contains 4 anthraquinones such as aloin, which easily discolors, which poses stability problems when added to cosmetics, as well as odor, color, and
Precipitates are also a big problem, so the range of their use is limited, and the amount they can be blended is also limited.

The present inventors have conducted intensive studies on external compositions such as skin cosmetics that have an excellent effect of promoting wound healing.
By subjecting the aloe sap to activated carbon treatment using the Kechi method, and then subjecting it to either or both of the following treatments: a desired heat treatment and an extraction treatment of extracting with a water-soluble organic solvent and collecting the extract. Aloe content obtained
It has been found that remarkable effects can be obtained when vitamin E, ie, vitamin E or a substance containing vitamin E, is used in combination with 1i. That is, the present inventors conducted various experiments such as animal experiments and human use tests in order to obtain a composition that has a healing effect on wounds, cracks, chapped skin, and other rough skin caused by beard hair. As a result, the aloe fraction was found to have all excellent physiologically active effects and a wide range of healing effects. In some cases, the healing effect of vitamin E cannot be obtained.In addition, vitamin E alone does not provide sufficient Ntlt.
Although no J% effect was observed, - When the aloe fraction and vitamin E were used together, they acted synergistically, particularly slowing down healing in animal experiments. It has a remarkable synergistic effect in plants with a long growing season, exhibits a more pronounced wound healing effect than when used alone, and is also reliable against refractory diseases (K1'PP, senile xeroderma, etc.) The present invention has been made based on the discovery that this drug exhibits a significant healing effect.

Hereinafter, the present invention will be explained in detail.

The external composition of the present invention contains vitamin E or vitamin E.
The substance containing the aloe vera and the aloe liquid are treated with activated carbon using a patch method and, if desired, subjected to either or both of heating treatment and extraction treatment of extracting with a water-soluble organic solvent and collecting the extract. It is made by using together with an aloe fraction obtained by.

Here, vitamin E and substances containing it include vitamin E, vitamin E acetate, tocopherol, α-tocopherol + dt-α-tocopherol,
Examples include wheat germ oil and rice germ oil, and one or more of these can be blended.

The amount of vitamin E in the composition is not necessarily limited, but it is preferably 0.001 to 10% (1% by weight, same hereinafter), particularly 0.1 to 5% in the composition.

The composition related to Honsaki ψ is a combination of the above-mentioned vitamin E and a specific aloe fraction. This aloe fraction contains no water-insoluble substances and does not contain BJ-soluble substances in water. It is a stable colorless to yellowish-yellow fraction with no bitterness and taste, and it does not have any severe effects such as wound healing and menstruation, and its stability in use is poor. be.

To explain in more detail the method for producing this aloe fraction (physiologically active substance from aloe), Aloe pera, soap aloe, and Kidachi aloe are preferably used as raw materials. In addition, Aloe mi can be obtained by squeezing, crushing, crushing, etc. the whole plant or a part of the jelly excluding the sweet part or the sweet part of the aloe.

In the method for producing the aloe fraction used in the present invention, the sap obtained by squeezing, crushing, crushing, etc. the whole aloe plant, beam part or part of the jelly is used. Even if you crush, crush, etc. and send it to the next process as it is, that is, the solid proboscis mixed with the sap, it will not be supported. However, it is possible to remove the solid matter by an appropriate separation means such as centrifugal filtration, filterless tFi filtration, natural filtration using P paper or oven cloth, etc., and use only the sap in the following steps. desirable from the viewpoint of operability, etc. Note that if the solid matter is not removed at this stage, it will be removed at a later appropriate stage.

Next, the aloe fraction thus obtained is treated with activated carbon by a patch method. This activated carbon treatment reliably removes colored components that easily discolor, and reliably yields a colorless to pale yellow, non-bitter, water-soluble substance that has physiologically active effects such as wound healing. On the other hand, when activated carbon treatment is not performed, colored components and water-insoluble components are not removed well, and this is not suitable for the use of the present invention.

As mentioned above, activated carbon treatment is carried out using a patch treatment method in which activated carbon is added to aloe sap and stirred. Because of the high content of inorganic substances such as sodium chloride in the resulting fraction, there is a risk of pain when applied to a wound site, which poses a problem in terms of usability. . Moreover, since water-insoluble components are not completely removed and inflammatory substances are included, activated carbon column chromatography cannot be employed in the application of the present invention. On the other hand, by performing activated carbon treatment using the patch method, a relatively small amount of activated carbon can be used, reducing the amount of activated carbon used and lowering costs. The content of inorganic salts can be lowered to improve pain during external application, and water and
1. It can almost completely remove soluble substances, reliably remove inflammatory substances, and improve water solubility, as well as reliably remove colored pigment components that easily change color, and has no bitter taste. This makes it possible to reliably obtain a fraction with good taste.

The old charcoal used in this activated carbon treatment using the patch method may be animal charcoal such as animal charcoal or bone charcoal, or other types of charcoal such as coconut shell charcoal, but the adsorption power is poor and inexpensive vegetable charcoal is preferred. It is preferable. The activated carbon may be powder or granule, but powder, particularly 100 to 400 mesh, is preferred. The amount of activated carbon used is preferably 0.1 to 20 inches (kk%, same hereinafter), preferably 1' to 10 inches, based on the weight of the original aloe sap. In activated carbon treatment by the patch method, after adding activated carbon, it is preferable to stir the liquid by means such as mechanical stirring, but in this case, the stirring time is preferably 15 minutes to 3 hours, particularly 30 minutes to 1 hour. preferable. Note that the activated carbon treatment can be performed at low temperature or room temperature, and when performed simultaneously with the heat treatment described below, it is performed under heating.

After the activated carbon treatment, if other treatments are to be carried out thereafter, preferably the activated carbon-removed product is subjected to a prescribed treatment, and if the activated carbon treatment is carried out as the final step, appropriate treatment such as filtration is carried out. The activated carbon is removed by a separation means, and the furnace liquid is collected as a target fraction.

The target fraction obtained by the activated carbon treatment is almost colorless and transparent in the form of a treatment solution.

This target fraction has colored pigment components that easily change color and water-insoluble components removed, and has excellent physiologically active effects such as wound healing effects. The treatment liquid can be used as it is as a target fraction, but an appropriate drying method such as heat drying, spray drying, freeze drying, etc. can be used to distill off the water, resulting in a smooth white color. It is preferable to collect it as a pale yellow to pale yellow powdery substance from the viewpoint of storage stability and wide-ranging usability.

As described above, the aloe fraction used in the present invention is obtained by treating the aloe sap with activated carbon using the patch method, and then removing the treated solution or water and collecting the powder obtained as the desired fraction. In this case, it is preferable to further perform a heat treatment in addition to the above-mentioned activated carbon treatment.This heat treatment further reliably removes water-insoluble components, and also removes components that become water-insoluble due to calothermal denaturation or that undergo denaturation over time. Components that become water-insoluble (flame-causing substances) are also reliably removed. Therefore, by performing heat treatment in addition to activated carbon treatment, water-insoluble substances and substances that denature due to heating or aging (flame-causing substances) are removed. A stable physiologically active substance that is removed and does not change over time is obtained. In addition, the heat treatment eliminates the viscosity of the aloe sap, making the purification process extremely easy, which is advantageous in terms of operability.

In addition, when performing heat treatment and activated carbon treatment by patch method, either heat treatment or activated carbon treatment may be performed first, or they may be performed at the same time, but it is better to perform activated carbon treatment after heat treatment. Alternatively, it is preferable to perform the heat treatment and the activated carbon treatment at the same time.

The heat treatment is performed at a temperature of 50°C under normal pressure or reduced pressure.
It is desirable to carry out the heating at ~90°C, preferably 60~8°C.As mentioned above, this heat treatment more reliably removes water-insoluble components and colored components, and also removes water-insoluble components by heat denaturation and denaturation over time. Ingredients (inflammatory substances)
is removed.

In addition, the heating time is 05 to 3 hours, more preferably 1 to 2 hours.
It is desirable to take an hour. If the heating temperature is lower than 50'C or the heating time is shorter than 30 minutes, the effect of the heat treatment will not be fully exhibited, and the above-mentioned impurity components will not be sufficiently removed in the process of separating insoluble matter. There may be cases where the value is not 0. Furthermore, if the heating temperature is 90"0.1 higher, or if the heating time is longer than 3 hours, problems such as deterioration and fermentation of the active ingredients and decomposition and solubilization of insoluble materials may occur.

When performing the heat treatment, the aloe sap may be simply heated under the above treatment conditions, but the aloe sap can be concentrated at the same time when performing this heat treatment (in this case, the aloe sap is Less than 10 times the amount of sap,
(It is more preferable to use a concentrated solution of 2 to 5 times the strength.)Also, there is no problem even if the moisture is almost completely vaporized.

After Calo heat treatment, if this is done at the final stage, water insoluble matter is removed by means such as filtration, and the target fraction is collected, and after the heat treatment, it is treated with activated carbon or extracted with a water-soluble organic solvent as described below. If you are going to process it, it is one of them! or water-insoluble substances should be removed. In addition, when performing activated carbon treatment after heat treatment, if +iyt liquid is concentrated in this heat treatment process, add water to it, preferably to make a concentrated liquid with an amount of 2 to 10 times the initial amount of sap. In addition, it is preferable to add activated carbon to this.

When obtaining the aloe fraction used in the present invention, in addition to the above-mentioned activated carbon treatment, or in addition to the activated carbon treatment and the heat treatment, an extraction treatment with a water-soluble organic solvent can be performed. The extraction process allows resins and other poorly soluble substances to be removed more reliably.

This extraction treatment may be performed at any stage before or after the activated carbon treatment or heat treatment, but especially when performing the above-mentioned king treatment, it may be performed before or after the activated carbon treatment after the heat treatment. It is preferable to carry out the extraction treatment after I″i, or before or after the step of simultaneously applying Calo heat treatment and activated carbon treatment.

In addition, when extraction treatment with a water-soluble organic solvent is performed prior to activated carbon treatment or heat treatment, the organic solvent is added to the aloe sap and the extraction is performed using a bamboo paste, and the extract is collected and the organic solvent is distilled off. The organic solvent is distilled off and water is added to the ζ mixture, which is then sent to the next process (activated carbon treatment process, heat treatment process).

When extraction treatment with a water-soluble organic solvent is performed after activated carbon treatment, extraction is carried out by adding an organic solvent to the treated liquid obtained by removing activated carbon, preferably its concentrated liquid or dried product, and heat treatment. In the case of extraction after heat treatment, an organic solvent is added to the sap after heat treatment, preferably its concentrated liquid or heat-dried product, and after extraction, the extract, its concentrated state, or its solvent-distilled product is collected as described above. It is something to do. In addition, when extraction treatment is performed as the final step, it is preferable to distill off the solvent from the obtained extract and collect it as the desired fraction, but depending on the purpose of use, the solvent may be distilled off. The extract or its cold crude liquid can also be used for sweetening (particularly when ethanol or inzolopanol is used as the water-soluble solvent).

In this extraction step, the organic solvents used for extraction include ethanol, methanol, inglono9ol, n-propyl alcohol, n-butyl alcohol, tert-
A water-soluble solvent such as butyl alcohol, sorbitol, acetone, etc. is used, and it is particularly preferable for the extraction treatment to use a mixed solvent with water containing these water-soluble solvents at a concentration of 20 to 80 φ, more preferably 25 to 40%. In this case, the water-soluble solvent may be used alone or in combination of two or more. In addition, the amount of organic solvent used (water-soluble solvent, especially mixed solvent of water-soluble solvent and water) is 0.1 to 30% of the substance to be extracted in the organic solvent.
More preferably, the concentration is 1 to 10%. The preferred extraction conditions are a temperature of 0 to 15° C. and an extraction time of 1 hour to 3 to 48 hours, preferably 3 to 48 hours. After the extraction process, the number of extractions is collected.

The target fraction obtained by performing the extraction treatment with this water active organic solvent contains resins, etc. Alcohol-poorly soluble substances are actually removed.

Therefore, the objective 11 items obtained by the above method are as follows:
It exhibits a very high healing promoting effect in response to the side temperature of hot water or boiling water, and has a tissue activation effect similar to goldstone.It also contains no colored pigment components or water-insoluble components that easily change color, and is colorless to pale yellow. In particular, those that have been fully treated with IJII olfactory treatment ensure that water-insoluble components (inflammatory substances) due to heat denaturation and denaturation over time are removed, and are stable and do not discolor or deteriorate in quality. Products that have been extracted with water-soluble organic solvents do not contain alcohol-insoluble substances, and even if they are blended into various products, their appearance will not be affected, causing problems in terms of product quality. There's no fear.

Moreover, this physiologically active substance is water-soluble and can be added to water at an almost arbitrary ratio of 8%, and can be mixed with ethanol or iso7'o/
It is also dissolved in a mixed solvent of water and glycerin, globylene glycol, etc., and the target fraction obtained by extraction with a water-soluble organic solvent has a Since it has a high f1 history, it is easy to use when distributing it to products and can be easily prepared into various dosage forms.

Moreover, this fraction is highly safe and has no stinging (7r) effect even when applied to skin problems.

In addition, the combination of aloe fractions is not necessarily:! j! I, but not limited to, the entire composition (1001 ~; 30, especially (
)01 to 10% is preferred.

The composition for external use of the present invention contains oils and fats
Hydrophilic ointment, water-absorbing ointment W), water-soluble ointment, ointment such as Gel ointment 11, emollient cream, cleansing cream, foundation cream, Matusano cream,
Vanishing cream, Nutriend cream,
Creams such as No1 and Cream, Lean Screen Cream, 7 Aping Cream, Emollient Comb Sun, Cleansing Lotion, Ng Aung 7' Ying Long rJ
- Loshishin 1 soaps, packs, face powders, powders, talcum, etc. powder, baby powder, 7j oP
Powders such as Landhowder, lipsticks, sticks such as deodorant Stay Sok, louche, nail polish, eye cosmetics, sprays such as deodorant spray, Nonosu salt, lotus oil, 7yan 7'-,
It can be prepared and used as a rinse, hair tonic, hair conditioner, hair oil, hair cream, pomade, setting lotion, hair gray, glossy waving lotion, hair dye, other hair cosmetics, and the like.

In this case, the composition may be aqueous type, alcohol type, W
10 emulsion type, 0/W emulsion type,
Multilayer emulsion tights with fractional aspects of Wlo, 'W can be prepared in the form of powder type, stick type, spray type, paste tights, etc., with other known ingredients depending on the type of composition to be prepared. ◇For example, distilled water, deionized water, ethanol, inglobil alcohol, glycerin,
polypropylene glycol, polyethylene glycol,
Polyhydric alcohols such as sorbitol, animal oils, vegetable oils, mineral oils, hydrogenated oils, waxes such as carnauba wax and beeswax, liquid halafine, higher hydrocarbons such as wax and rough-in wax, fatty acids such as stearic acid, emulsifiers, Surfactant, tracanthin gum, xanthan gum, sodium alginate, methylcellulose, hydroxyethylcellulose, sonoumcal? quinmethylcellulose, cal-d
? Water-soluble polymer compounds such as xyvinyl polymer, talc, kaolin, calcium carbonate, hydrogen phosphate,
Starch and other non-toxic carriers are selectively used depending on the type of cosmetic. of'? -Fuyume, flavor, color, sweetener and preservative may also be added as required. Furthermore,
Other active ingredients such as vitamins, hormones, antihistamines, astrinol, enzymes, hinokitiol, β-cholestanol, ultraviolet absorbers, and bactericides may also be incorporated.

In addition, in the composition of the present invention, there is no major problem even if ingredients that are not necessarily considered to have a good effect on wound healing, such as strong fragrances, are used, and any formulation can be used. It can be commercialized with Moreover, according to the present invention, a good wound healing effect can be obtained even when a base material that suppresses wound healing is used. Compositions with different formulations can be easily produced.

Next, production examples and comparative production examples of aloe fractions used in the present invention will be shown.

[Manufacturing Example 1] Crush 10 kli+ of Kidachi Aloe beams with a mixer,
1 kg of powdered activated carbon was added to this, stirred at room temperature for about 30 minutes, and then filtered. The p liquid was concentrated under reduced pressure with an aspirator at a bath temperature of 40°C, and further vacuum-dried.
Fraction 1) 175. (I got l.

[Production Example 2] Crush 10 kli of Kidachi Aloe beam part with a mixer,
Filtration through a cotton cloth and natural filtration through a filter paper were performed to obtain 7.5 kg of slightly opaque yellow-green sap. Apply 7.5 units of this sap to a person at 300 + mnHp under reduced pressure f 10 'C1
After heating for 30 minutes, 3.7 kg of concentrated sap was obtained. 30Of powdered activated carbon was added to this, and after stirring at room temperature for about 30 minutes, it was suction filtered using P paper, and then the activated carbon was washed with water and the washing liquid was added to P solution. This p solution is heated to a bath temperature of 40°C.
It was concentrated under reduced pressure using an aspirator and further dried in vacuo to give a white powder (fraction n) 172. I got Of.

[Manufacturing example 3] Aloe pera beam part 10kl? food slicer, pal,
? -, finisher - processed in the order of green sap 7.6
I got Yu. 76 o2 of powdered activated carbon was added to this sap, and after stirring at room temperature for about 1 hour, it was filtered using a filter press machine. This F solution was concentrated to about 1/21 while heating it to 70°C, and the water-insoluble matter generated by heating was naturally filtered using P paper. This P solution was spray-dried, and a white powder ( Fraction IVI) 72.2t were obtained.

[Manufacturing Example 4] Place 10 kg of aloe vera beam in a food slicer, pulper, and Finnish jar.
I got kg. Add powdered activated carbon 7602 to this sap,
The mixture was stirred for about 1 hour while heating to 70°C, then allowed to cool to room temperature, and filtered by absorption using filter paper.

Next, the activated carbon was washed with water, and the washing solution No. 7 was added to the p solution.

This P solution was spray-dried to give a white powder (fraction IV) 72.
2r'ff: Obtained.

[Manufacturing Example 5] Put 10 kg of Kidachi Aloe beam into a food slicer. Processed in order of ruber and finisher, green sap 7.7
I got ky. Powdered activated carbon 7002 was added to this sap, stirred at room temperature for about 30 minutes, and then filtered using a filter press. This P solution was concentrated under reduced pressure using an aspirator while heating the bath temperature to 40°C until the concentration reached 1.6.
kg of concentrate was obtained.

0.81% of ethanol was added thereto, stirred for 30 minutes, and allowed to stand at room temperature for about 1 day. The precipitated white precipitate was suction-filtered using P paper to obtain liquids L and F. This bath temperature is 40
The mixture was concentrated under reduced pressure using an aspirator at °C and further dried under vacuum to obtain a white yellow powder (fraction V) of 116.0V.

[Production Example 6] / 10 kg of aloe leaves were crushed in a mixer, filtered through cotton cloth, and naturally filtered through P paper to obtain 6.6 kg of yellow-green, slightly opaque sap.

This sap was freeze-dried to obtain 237.6y of yellowish brown powder. To this was added 2 tons of 33% ethanol aqueous solution, and after stirring for 60 minutes, the mixture was left to stand still at room temperature for 1 day. The precipitated pale yellow precipitate was filtered using P paper to obtain a reddish brown P solution.

This p liquid was concentrated to dryness while heating at 70°C, and a yellowish brown extract of 133% was obtained. (I got l.

To this was added 220 (1++t) water and 18 Or powdered activated carbon, and after stirring at room temperature for about 30 minutes, -C absorption filtration was carried out using filter paper. Then, the activated carbon was washed with water, and the washing liquid was added to P solution. The filtrate was freeze-dried to obtain a pale yellow powder (fraction Vl) 101.Oj'.

[Manufacturing Example 7] Crush 10kli+ of Kidachi Aloe beams with a mixer,
Filtration through cotton cloth and natural filtration through P paper were performed to obtain 7.5 liters of slightly opaque yellow-green sap. Next, this sap was heated to 70°C and concentrated to about 1/3 of its original volume to obtain 2.5 kg of condensate. 4. Add 2602 powdered activated carbon to this for about 30 minutes. After stirring for 1'L, suction filtration was performed using a paper towel, the activated carbon was washed with water, and the washing liquid was added to Solution F. Add 1.3 t of methanol to this P4'' and stir for about 30 minutes at 1 ttJ, then at room temperature
It was left undisturbed for a day. The white precipitate produced was suction-filtered using P paper money to obtain a colorless and transparent solution.The P liquid was concentrated in an aspirator pressure mode, methanol was distilled off, and the powder was dried. , white powder (fraction ■) 9 (1,79 was obtained.

[Manufacturing Example 8] Treat 10 kg of the two beams of Kidachiaro in the order of food slicer, Palzya, and Finitsunya, and add green No. 4 J liquid 7.
．． I got 7 kg. Powdered activated carbon (385 V) was added to this tree for color, and the powder was heated at room temperature for about 30 minutes (after stirring, it was filtered through a filter Sores filter. While heating this Po to 70 "O", it was heated to about 175 V) until Q. 1.6 kg of dairy
A concentrate was obtained. This was naturally filtered using tfP paper, and p
0.86% of ethanol was added to the solution, stirred for 30 minutes, and allowed to stand at room temperature for about 1 day. The precipitated white precipitate was suction-filtered using P paper to obtain a colorless and transparent P solution. This bath temperature 4
Concentrate under reduced pressure with an aspirator at 0°C and further dry in vacuum to obtain a white powder (1111 fractions X).
f I got it.

[Production Example 9] 10 tons of water was added to 10 kg of aloe pera beam, crushed in a mixer, heated at 70°C for 1 hour, filtered naturally using a door paper, and then distilled off the water under reduced pressure with an aspirator. , tan powder 150. I got Or. To this was added 2.25 liters of a 33% ethanol aqueous solution, and after stirring for about 1 hour, it was allowed to stand at room temperature for about 1 day. Next, the precipitated pale yellow precipitate was removed using paper towels to obtain a reddish-brown ν liquid. This p liquid was concentrated to dryness under reduced pressure using an aspirator, and a yellowish brown extract of 112. I got Of. 3 tons of water and 300 tons of activated carbon were added thereto, stirred for about 30 minutes, and then filtered with a centrifugal filter using P paper to obtain a colorless and transparent solution. Next, this solution was concentrated under reduced pressure using an aspirator at a bath temperature of 40°C, and further vacuum dried to obtain a white to light yellow powder (fraction ■) 81, Of'! il-obtained.

[Comparative Production Example 1] ゛10 kg of Aloe aloe leaves were crushed in a mixer, filtered through cotton cloth, and naturally filtered through P paper to produce 7.5 kl of slightly opaque yellow-green sap. The obtained sap was freeze-dried to obtain a yellowish brown powder (comparative product I) 2102.

[Comparative Production Example 2] 10 collected fresh Aloe leaves were ground with a klij machine to obtain 7.9 kg of sap. 32 tons of ethanol was added to this sap, and the mixture was thoroughly stirred at room temperature and left to stand day and night, and the resulting precipitate was removed. Next, the supernatant ethanol extract was concentrated under reduced pressure at 40 to 50°C to obtain a yellowish brown solid 120f. Dissolve this in 10 tons of water and make approximately 2 kg
Chromatography was performed using activated carbon (Medjuro 0-80). Further, water 1O6 was further eluted, and the obtained aqueous solution meter 201 was spray-dried to obtain about 582 of a white comparative substance (comparative product ■).

Next, the characteristics of the fractions and comparative products obtained in the above production examples and comparative production examples will be explained using the following experimental examples.

[Experiment f? 1] Renzo 1ull t fractions obtained in 4.5.7 ■, ■,
■Comparative product 1. obtained in Comparative Production Example 1.2. II was blended into a hydrophilic base in an amount of 5 each, and the usability test was conducted. The panel selected 14 patients with cracked and chapped hands with cracks on their left and right hands, and tested the five samples mentioned above three times. The exam interval was one week.

The drug was applied to the affected areas of the left and right hands, and symptoms were observed for 30 minutes after application. Judgments were made based on the panel's sense of irritation, the onset of pain, and the degree of redness on a 4-level scale from severe to no response. The results are shown in Table 1.

Table 1 Fractions (1), (2), and (1) gave good results in the application of the soft powder blended with Q, but the comparative product (1) which was not treated with activated carbon or caloric heat was treated. Comparison product ■, which uses activated carbon column chromatography, has a high incidence of each symptom, especially comparison product 1.
More than half of the patients complained of irritation and pain.

Note that the irritation, pain, and redness were all temporary and disappeared after a few minutes.

[Experiment 2] 11. obtained in Production Example 4. The fraction ① and the comparative product 1 obtained in Comparative Production Example 1 were each mixed into a hydrophilic base at 10%, stored at 20°C for 3 months, and the degree of discoloration during that time was measured using a photoelectric whiteness meter. Measured using

The results (changes in Hunter's white discoloration over time) are shown in the figure. In the figure, ■ indicates the fraction ■, 1' indicates the comparative product ■, and B indicates the base material.

No oral changes were observed in the ointment containing the fraction of Production Example ① even after storage at 20°C for 3 months, but a comparative ointment containing the ointment that had not been treated with activated carbon showed significant discoloration.

[Experiment 3] An acute toxicity test was conducted using ICR male mice. A 25% suspension of the fractions obtained in Production Examples 1 to 9 and a comparative product obtained in Comparative Production Examples 1 to 2 and a 25% suspension of gum arabic was orally administered.

The results were Ll) 5o) 10,000 mfl/9 mice body weight.

Regarding Comparative Product I, loose stools were observed at low doses, and accompanying diarrhea symptoms were observed at high doses.

[Experiment 4] Hair was removed from the back of a male white rabbit (2.3-3.5 kg), and 1 hour, 3 hours, 6:01..., 24 hours, 4 hours after applying the sample.
Skin symptoms at 8 hours, 72 hours, 96 hours, 168 hours, and 336 hours were scored based on the Dralze method.

For the specimen, we used a soft sachet and aqueous solution containing fractions I to ■.
The concentration of ointment was 5% and 10%, and the concentration of aqueous solution was 5%, 10% and 20%. In addition, the ointment base and distilled water were applied as a control.

As a result, skin llI4 irritation was not observed in any of the fractions.

[Experiment 5] The mutagenicity of fractions I, 1.111, and ■ was determined in real M11 using the Ames method (grain cupation method). Mutagenicity is Sxlmonella typhlmurlum
TA98 and TAloo, 1112 and E, Co11
The three WP2 uvrA were used as test bacteria.

As a result, the valley fraction was not found to be mutagenic.

EXAMPLES Hereinafter, the present invention will be explained in detail with reference to Examples and Comparative Examples.

[Examples 1-2, Comparative Examples 1-4] The creams shown in Table 2 were manufactured by tA, and their healing effects on 713 wounds were investigated.

NIJ 1% healing effect using tensile strength as an index Wistar rats (SPF) A group of 6 rats was anesthetized with bentoparbital sodium, the hair on the buttocks was extensively shaved, the hair was disinfected with alcohol, and the midline was cut with a scalpel. A 4 cm sharp linear wound was made in the center of the rat's back along the line, and sutured at 3 points every 1 ctn using a Mitsuhel needle.

After the wound was prepared, the cream was continuously applied to the wound twice a day for 7 days. On the 8th day, the rats were killed with chloroform, the skin at the wound site was exfoliated, the subcutaneous tissue was removed, and three 1131 cm pieces of skin perpendicular to the wound line were prepared from each rat using a razor. The tension (f/
Cm: Ten5l le strength value) was measured, and the average value was used as an index of the wound healing rate.

The results are shown in Table 3.4. The results are Tsnsll when the control group (cream application of Comparative Example 1) is set as 100.
e strength is expressed directly.

Table 3 Table of 4 results when using Lieutenant Colonel 5 Tokurei rats
Based on the results of B.1 or more when using raw Buddha's 50-week-old foot gold, the combined use of vitamin E and AFF fractions resulted in excellent QIJ i4 healing (providing Jt healing effect and improving bamboo growth). It was also found that it had a remarkable effect on the Ikua Nen'ei style, which is slow to heal.

Note that other aloe fractions according to the present invention are also used in actual Mt example 1.
Results similar to those of 2 were obtained.

[Example 3] Cleansong cream paraffin 50% cetanol 15 Vaseline 18.0 style Sukenoya rough in 280
Glyceryl monostearate
3.0 polyoxy/ethylene monolaurin H1'i/,
3.0 glycerin
:30 retinol
50,000 UH fee ()2 Aloe fraction'proboscis 1 1
1.5 vitamin E
O, 1 no 9 Utki/Abjoya Hakko Methyl
Butyl 005-4-raoxybenzoate
(105100,0% [Example 4] Oil-based cream beeswax 10%flu
mJ no 9 rough in 50 borax
1.0 allotechnical fraction III
O,1 wheat germ oil
0.5 water
Remaining [Example 5]
Lotion glycerin 50%
Propylene glycol
40 oleyl alcohol
01, Jζ lipoquin ethylene sorbitan monolaurin + * t, S, 45 lyoxyethylene lauryl edel ()5 ethanol
IO30 Allo Engineering Bunkokumono v
10α-tocopherol
0.6 vitamin B6 dipalmitate ester ()1 fragrance
01t
R Laoki 7 Butyl benzoate 0.0
5LO0.0% [Example 6] Skin cream stearic acid
14% work I77
”・0 Glyceryl monostearate 25 Polyoxyethylene sorbitan mono 15 Stearin 1 Tone ester globylene glycol
lO aloe fraction■
0゛2dt-α-tocopherol 0.
4AD-Oil 0.2 [Example 7] Emulsion steari:/acid
20% cetanol
15 Vaseline
3.0 lanolin alcohol
20 is L1vJJ Norifin 10
．． 01j Solioxyethylene monooleate ester
20 glycerin
30 globylene glycol
50 triethanolamine
1.0 allotechnical fraction C<
(1,5 vitamin E acetate
0.2) e Laoquin benzoin ethyl (1, CI !5 paraoxybenzoin ethyl OO 5th grade)
0.5 Wed Pleasant [actual h]
11] 1ri 1] 8] No. Cream Stearic Acid 170% Glythyl Stearate 3.0 7 Licorn L River
(+3 polyoxyethylene oleyl alcohol 70 glycerin
60 allotechnical fraction ■2.0 Rice germ oil 12
β-carotene U,
iPreservative 0
1st Sergeant (
11101J, 0chi [Including row 9] Drillable soft granite, 5
% squalane
8.5 Kudo Parafuino
10.0 petrolatum
225 Polyoxyethylene sorbitan heptanooleate 1.5 Sorbitan monoole L/-) 3.5 Butyl paraoxybenzoate
fl, 05 allo engineering parts ■
:30dt-α-tocopherol
051 (10,Q% [Implementation 1 IJ 10) Ointment Macrogol 400 (150,0% Macrogol 400 4B
, 7 allotechnical fraction Vl
1.010 (1.0% [Example 11) Kaolin 560
% Glycerin 382 Salicyl 1 Wing Methyl 0.
2 tsp oil 0.5
Allotechnical fraction Vt1l
5.0100.0% [Example 12] Hair tonic alcohol 59.
0 exposed glycerin 5,
0 salicylic acid
0.3 stearyltrimethylammonium chloride
1.0 Allotechnical fraction 1.0 Acetic acid dt-α-] Copherol 0
．． 05 fragrance total 100.0%

[Brief explanation of drawings]

The figure is a graph showing the change over time of the aloe fraction of the present invention in a preparation with a hydrophilicity of 4 and the composition of the base. Applicant Lion Co., Ltd. Agent Patent Attorney Takashi Kojima November 1989 Amendment to the number of procedures (text) Showa 57th September 11 Showa 57:1! Ik, rr Kn No. 12411 (
Name of No. 4 250 Ming External composition 3 Corrector Relationship with month 1 'l'l' i! 'i applicant f
1-341i 7 Honjo, Matsuda-ku, Mito
'J(1) Specification 11 (Page 1, line 20, ``Skin 1i 6 minutes secretion stimulation j'' is changed to ``Expenditure secretion stimulation I le''. (2) Same 3rd shell No. 15 The line ``Wound Tano J dumb'' is written as ``R fill wound night is S7 effect'' and J1 stops. (3) The 12th line of the same No. Stirring" and d] stop. (4) Stop "finisher" in line 10 of No. 21 of the same. Imo-sou 3 Nashi”
"bentoparbital sodium" is changed to "bentoparbital sodium" in 271. that's all

Claims (1)

[Scope of Claims] 1. Vitamin E or a substance containing vitamin E and activated carbon treatment of aloe side liquid by a batch method.
and, if desired, an aloe fraction obtained by performing either or both of heat treatment and extraction treatment of extracting with water-03@isosol solvent and collecting the extract. A composition for external use characterized by: 2. The content of vitamin E or a substance containing vitamin E is 0.001 to 10% of the total, and the blend I of the aloe fraction is 0.01 to 5 tft% of the total. The composition according to item 1.