KR102186984B1 - A composition for promoting melanocyte migration comprising Cinnamomum camphora extract - Google Patents

Abstract

The present invention provides a skin external preparation or cosmetic composition, which can make spotted skin colors even through the development of efficacy through the effect of melanocyte migration of a Cinnamomum camphora extract, can alleviate pigment loss, such as progressive macular hypomelanosis, and even can be applied to pigment loss disorders such as vitiligo in which cells are destroyed.

Description

A composition for promoting melanocyte migration comprising Cinnamomum camphora extract}

The present invention relates to a composition for promoting melanocyte migration containing camphor tree extract.

The pigments that determine skin color include melanin, hemoglobin, and carotenoids. These pigments determine the color of skin, hair, and eyes. Among these, melanin is the most important pigment that determines skin color, and skin color appears differently depending on its properties and distribution. Melanin is produced by melanocytes, and the generated melanin serves to protect the skin by removing oxygen radicals formed in the skin.

There are diseases in which the skin color is spotted or the pigment is lost due to abnormal distribution or function of melanocytes, such as dry ringworm, progressive spot pigmentation, and vitiligo.

Among them, vitiligo is a representative incurable disease in which skin color disappears. First, ultraviolet light (ultraviolet irradiation device or excimer laser) is used in the treatment of vitiligo. The treatment effect is good, but the treatment effect is incomplete, it is easy to get burned, and skin aging is accelerated. Second, it is used when a small area is invaded by steroid therapy, and methods such as topical application or injection are used. Third, epidermal transplantation and cell transplantation are attempted as surgical treatments, but they are not yet universally performed and cannot be treated in a wide range. Fourth, a method of covering with dark makeup and treatment with complex vitamins are being tried, but there is no good treatment method yet.

Phototherapy, the most standard treatment method, is a method that activates melanocytes in the hair follicles and moves them to the epidermis to expect a therapeutic effect, so if it can promote the movement of melanocytes, the therapeutic effect can be increased. Little is known about the material that shows.

Looking at patents related to the treatment of vitiligo, etc., a cosmetic composition for relieving vitiligo (patent application No. 10-2007-0048916), a composition for preventing white hair and treatment of vitiligo containing black root (patent application No. 10-2007-0119266), benzenediol Vitiligo treatment consisting of a (benzenediol) derivative (Patent Application No. 10-2007-0048916), a pharmaceutical composition for treatment of vitiligo containing Criftansus extract as an active ingredient (Patent Application No. 10-2009-0090079), linolenic acid A pharmaceutical composition for treating vitiligo containing as an active ingredient (Patent Application No. 10-2011-0080566), a composition for preventing white hair and treating vitiligo containing ginseng fruit extract (Patent Application No. 10-2011-0078361), Pueraria genus A composition for preventing and treating white hair or vitiligo containing a plant extract or puerarin (Patent Application No. 10-2011-0100393), a composition for preventing and treating white hair containing a propanoid derivative (Patent Application No. 10-2012- 0015823), composition for treatment or prevention of vitiligo containing Yongan meat extract (patent application No. 10-2012-0129576), composition for prevention or treatment of vitiligo (patent application No. 10-2014-0019276), composition for promoting melanin synthesis (Patent Application No. 10-2016-0110716) and the like.

However, these inventions are usually pursuing the treatment or prevention of vitiligo by promoting melanin synthesis, and little is known about the effect of treating or alleviating vitiligo through cell migration.

On the other hand, camphor tree is an evergreen broad-leaved arboreous tree belonging to the magnolia family camphor tree, distributed in Vietnam, Taiwan, and Japan, and grows wildly in Jeju Island. The height is about 20 m and the young branches are yellow-green. Leaves are alternate and glossy, and three veins are lined up, and young leaves are slightly reddish. Camphor (樟腦), which is made by distilling wood, branches, leaves, and roots into steam, is used as a tonic or stimulant. It is made into an injection and is used as a stimulant for the respiratory center, blood vessels, nerves, center, and heart. It is also used as a material for insect repellent, preservative, and fragrance, and wood is used as a building material or furniture material.

Camphor tree is known to have a strong antioxidant effect (J. Anal. Methods Chem.; 2019:4892635. doi: 10.1155/2019/4892635. eCollection 2019), and good anti-inflammatory effects have been reported in animal experiments (J. Food Sci. . 2016; 81(5):H1295-300.doi: 10.1111/1750-3841.13271.Epub). However, there were few cases of applying camphor extract to cosmetics, and in particular, no effects on melanocytes and pigment loss diseases have been reported.

1. Korean Patent Application No. 10-2007-0048916 2. Korean Patent Application No. 10-2007-0119266 3. Korean Patent Application No. 10-2007-0048916 4. Korean Patent Application No. 10-2009-0090079 5. Korean Patent Application No. 10-2011-0080566 6. Korean Patent Application No. 10-2011-0078361 7. Korean Patent Application No. 10-2011-0100393 8. Korean Patent Application No. 10-2012-0015823 9. Korean Patent Application No. 10-2012-0129576 10. Korean Patent Application No. 10-2014-0019276 11. Korean Patent Application No. 10-2016-0110716

1. J. Anal. Methods Chem.; 2019: 4892635 2. J. Food Sci. 2016; 81(5): H1295-300

In the present invention, it is possible to make spotted skin color uniform through the development of efficacy through the effect of melanocyte migration of camphor extract, and it is possible to improve pigment loss such as progressive spotting, and even cell destruction such as vitiligo. It is intended to provide a method for preparing camphor extract that can be applied to pigment loss diseases, and a skin external preparation or cosmetic composition comprising the extract.

According to a representative aspect of the present invention, there is provided a functional cosmetic composition for the treatment of vitiligo comprising camphor extract or a fraction thereof as an active ingredient.

According to another representative aspect of the present invention, there is provided a quasi-drug composition for treatment of vitiligo comprising camphor extract or a fraction thereof as an active ingredient.

According to the present invention, it is possible to make the spotted skin color uniform through the development of efficacy through the effect of melanocyte migration of camphor tree, and it is possible to improve pigment loss such as progressive spotting, and even cell-destructed vitiligo and The same can be applied to pigment loss disorders.

1 shows the effect of promoting the migration of melanocytes using camphor extract. Compared with normal, it shows the findings that the migration of melanocytes is promoted.
2 shows the result of improving skin bleaching disease through long-term use of a coating agent containing camphor extract. The left and right photographs of FIG. 2A show the results before and after application of the external preparation for skin, and FIG. 2B is an enlarged view.

Hereinafter, various aspects and various embodiments of the present invention will be described in more detail.

One aspect of the present invention relates to a functional cosmetic composition for treating pigment loss diseases comprising camphor extract or a fraction thereof as an active ingredient.

At this time, the extract or a fraction thereof promotes melanocyte migration.

As described above, in the present invention, the antioxidant efficacy of camphor tree extract and the effect on cell migration in normal melanocytes were observed, and the present invention described below was completed based on this.

As described above, camphor is an evergreen broad-leaved arboreous tree belonging to the magnolia camphoraceae, and is distributed in Vietnam, Taiwan, Japan, etc., and grows natively in Jeju Island. The height is about 20 m and the young branches are yellow-green. Leaves are alternate and glossy, and three veins are lined up, and young leaves are slightly reddish. Camphor (樟腦), which is made by distilling wood, branches, leaves, and roots into steam, is used as a tonic or stimulant. It is made into an injection and is used as a stimulant for the respiratory center, blood vessels, nerves, center, and heart. It is also used as a material for insect repellent, preservative, and fragrance, and wood is used as a building material or furniture material.

In the present invention, the term "fraction" of camphor extract refers to a fractional extract obtained by extracting camphor extract with an organic solvent or a fractional extract obtained by purifying the extract by chromatography.

In the present invention, the pigment loss disease may typically include vitiligo, but is not limited thereto, and white dermatosis caused by a chemical substance, white dermatosis after laser treatment, hypopigmentation after inflammation, drop-like white dermatosis, melanin hypothyroidism , Peculiar white dermatosis and other diseases may be included.

In the present invention, the term "functional cosmetic (cosmedical, cosmeceutical)" refers to a product having a professional function in which a professional treatment function of a drug is introduced into a cosmetic product, and unlike general cosmetic products, a physiologically active effect and an effect are emphasized. For example, the functional cosmetics refer to products that help whitening the skin, products that help improve skin wrinkles, and cosmetics that help to burn the skin finely or protect the skin from ultraviolet rays.In the present invention, It refers to cosmetics that are burnt or have an improvement effect on hypopigmented diseases.

In addition, the composition for promoting the movement of melanocytes of the present invention is not particularly limited in its formulation, and depending on the formulation to be prepared, it may include a skin coating agent compounding component commonly used in the technical field to which the present invention belongs.

According to one embodiment, the camphor extract or a fraction thereof is included in 0.001 to 30% by weight based on the total weight of the composition.

The composition for promoting melanocyte migration of the present invention contains the camphor extract, and these are preferably contained in an amount of 0.001 to 30% by weight based on the total composition. When the content is less than 0.001% by weight, there is a problem that the effect is insignificant, and when it exceeds 30% by weight, there is a problem that the effect according to the amount of use is insignificant.

According to another embodiment, the functional cosmetic composition has one formulation selected from lotion, emulsion, cream, pack, essence, gel, powder, foundation, detergent, lotion, and essence.

As such, the cosmetic product comprising the cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, Soap, shampoo, patch for skin adhesion, gel for skin adhesion, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray may be formulated, but is not limited thereto. More specifically, flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, lotion, gel, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, ointment, stick, patch, spray, powder It may be prepared in a formulation, and may be a face wash and a bath, especially when used as a detergent composition.

The cosmetic composition of the present invention is a cosmetic composition that is common in the cosmetic field, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, interfacial agents. Activators, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles as well as cosmetics It can be used as a component of general cosmetics or functional cosmetics together with adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients commonly used.

In particular, considering that the main cause of the destruction of melanocytes is active oxygen, the composition for promoting melanocyte migration of the present invention preferably contains a light scattering agent, an antioxidant, and the like, and the formulation and additive components are not limited to the above contents.

According to another embodiment, the camphor tree extract is extracted with one or more extraction solvents selected from water and C ₁ to C ₄ alcohol. In this case, the C ₁ to C ₄ alcohol may be selected from methanol, ethanol, or a mixed alcohol thereof.

However, the camphor extract can be used without limitation, such as collected or commercially available, and the method of preparing the extract is conventional in the art, such as supercritical extraction, subcritical extraction, high-temperature extraction, high pressure extraction, or ultrasonic extraction. The extraction method can be used. After the camphor is pulverized to an appropriate size, it is put into an extraction container, and lower alcohol, or a mixed solvent thereof, preferably methanol or ethanol, is added and left for a certain period of time, and then filtered through a filter paper to obtain an alcohol extract. Then, it is freeze-dried and concentrated.

According to a preferred embodiment, the camphor tree extract is extracted by an extraction method comprising the following steps.

(A) ultrasonically treating camphor powder,

(B) performing an enzymatic decomposition reaction of the sonicated camphor powder with a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 1.5 to 2.5: 1 to 2,

(C) extracting the camphor powder subjected to the enzymatic decomposition reaction with a mixed extraction solvent in which ethanol, methanol, and water are mixed in a weight ratio of 1: 1 to 2: 0.25 to 0.75.

As described above, the camphor extract used in preparing the functional cosmetic composition or quasi-drug composition according to the present invention has a large difference in effect depending on the extraction method. Specifically, it is preferable to undergo ultrasonic treatment and enzymatic decomposition reaction before extraction, and in particular, it is preferable to use a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 1.5 to 2.5: 1 to 2. . In addition, in performing the extraction process on the camphor tree powder thus pretreated, it is preferable to use a mixed extraction solvent in which ethanol, methanol, and water are mixed in a weight ratio of 1: 1 to 2: 0.25 to 0.75. In the case of performing the pretreatment process as above, and performing the enzymatic decomposition reaction by configuring a mixed enzyme with the above enzyme and composition, and performing the extraction process by configuring a mixed extraction solvent with the above solvent and composition, It was confirmed that the antioxidant effect as well as the effect of promoting the movement of melanocytes was significantly improved compared to the case that was not satisfied with either one, and some cases were presented in the following examples.

Another aspect of the present invention relates to a quasi-drug composition for treating pigment loss diseases comprising camphor extract or a fraction thereof as an active ingredient.

In the present invention, the term "quasi-drug" refers to fibers, rubber products, or the like, which are used for the purpose of treating, alleviating, treating, or preventing diseases of humans or animals, and the action on the human body is weak or does not directly act on the human body, Non-instrument or machine or similar, as an article corresponding to one of the preparations used for sterilization, insecticide, and similar purposes to prevent infection, for the purpose of diagnosing, treating, alleviating, treating or preventing human or animal diseases It refers to items that are not instruments, machines, or devices among the items used, and items that are not instruments, machines, or devices among items used for the purpose of having a pharmacological effect on the structure and function of a person or animal. Also includes hygiene products.

In the present invention, the ingredients included in the quasi-drug may be added to the quasi-drug composition as it is or may be used together with other quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient can be appropriately determined according to the intended use.

In addition, the composition for promoting melanocyte migration of the present invention may further include a pharmaceutically acceptable carrier or excipient. Representative carriers or excipients include water, dextrin and physiological saline.

At this time, the extract or a fraction thereof promotes melanocyte migration.

According to one embodiment, the camphor extract or a fraction thereof is included in 0.001 to 30% by weight based on the total weight of the composition.

As described above, the composition for promoting melanocyte migration of the present invention contains the camphor extract, and they are preferably contained in an amount of 0.001 to 30% by weight based on the total composition. When the content is less than 0.001% by weight, there is a problem that the effect is insignificant, and when it exceeds 30% by weight, there is a problem that the effect according to the amount of use is insignificant.

According to another embodiment, the quasi-drug composition has the form of an external preparation for skin.

The melanocyte migration promoting composition of the present invention is used by adjusting the amount according to the use purpose and the condition of the application site, and as an example, it is used as a method of applying once to three times a day at a concentration of 0.001 to 100 mg/mL per day. do.

According to another embodiment, the external preparation for skin has one formulation selected from ointments, patches, gels, creams, and sprays.

The external preparation for skin is not particularly limited, but is preferably prepared in the form of an ointment, warning agent, granule, lotion, spray, patch, cream, powder, suspension, emulsion, tablet, syrup, gel or gel. Can be used.

According to another embodiment, the camphor tree extract is extracted with one or more extraction solvents selected from water and C ₁ to C ₄ alcohol.

In this case, the C ₁ to C ₄ alcohol may be selected from methanol, ethanol, or a mixed alcohol thereof.

According to a preferred embodiment, the camphor tree extract is extracted by an extraction method comprising the following steps.

(A) ultrasonically treating camphor powder,

(B) performing an enzymatic decomposition reaction of the sonicated camphor powder with a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 1.5 to 2.5: 1 to 2,

(C) extracting the camphor powder subjected to the enzymatic decomposition reaction with a mixed extraction solvent in which ethanol, methanol, and water are mixed in a weight ratio of 1: 1 to 2: 0.25 to 0.75.

As described above, the pretreatment process is performed as above, and the enzyme decomposition reaction is performed by configuring a mixed enzyme with the above enzymes and composition, and also performing the extraction process by configuring a mixed extraction solvent with the above solvent and composition. In the case, it was confirmed that not only the antioxidant effect but also the effect of promoting the movement of melanocytes was significantly improved compared to the case where any one of them was not satisfied, and some cases were presented in the following examples.

Hereinafter, the present invention will be described in more detail through examples, etc., but the scope and contents of the present invention are reduced or limited by the examples below and cannot be interpreted. In addition, if based on the disclosure content of the present invention including the following examples, it is clear that the present invention for which no specific experimental results are presented can be easily carried out by a person skilled in the art, and such modifications and modifications are attached to the patent. It is natural to fall within the scope of the claims.

In addition, the experimental results presented below are only representative of the experimental results such as the above examples, and the effects of each of the various embodiments of the present invention not explicitly presented below will be described in detail in the corresponding section.

Example

Example 1-1

The camphor was washed with purified water, dried, and pulverized to obtain fine camphor powder. 100 g of the fine camphor powder was sufficiently dissolved in an extraction solvent of 500 mL of 70% ethanol and left at room temperature for 72 hours. The precipitate of the mixed solution was filtered through Whatman filter paper and concentrated with a rotary pressure evaporator at 65° C. to remove ethanol. The concentrate was freeze-dried to obtain 1.2 g of camphor extract.

Example 1-2

The camphor was washed with purified water, dried, and pulverized to obtain fine camphor powder. The camphor fine powder was suspended in water and subjected to ultrasonic treatment for 10 minutes, and then the solvent was removed and dried to obtain ultrasonically treated fine powder. UL-Tech's SEEC-SONIC II product was used as the ultrasonic processor, and it was performed by generating ultrasonic waves at 20 kHz, 750 watt, and 20 amplitude conditions. In order to keep the temperature constant, ultrasonic treatment was performed by connecting a circulator water bath. 100 g of the sonicated fine powder was suspended in water, and citric acid was added to adjust the pH to 6. Here, 5 g of a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 2: 1.5 was added, and the enzyme decomposition reaction was carried out while homogenizing by stirring at a speed of 200 rpm at a temperature of 40 °C. I did. After completion of the reaction, the enzyme was deactivated by heating at 100° C. for 10 minutes, and the solvent was removed to obtain a camphor enzyme digest. The camphor enzyme decomposition product was sufficiently dissolved in 500 mL of a mixed extraction solvent in which ethanol, methanol and water were mixed in a weight ratio of 1: 1.5: 0.5, and allowed to stand at room temperature for 72 hours. The precipitate of the mixed solution was filtered through Whatman filter paper and concentrated with a rotary pressure evaporator at 65° C. to remove ethanol. The concentrate was freeze-dried to obtain 1.2 g of camphor extract.

Example 1-3

Camphor extract was obtained in the same manner as in Example 1-2, except that ultrasonic treatment was not performed.

Example 1-4

Cellulase, beta-glucanase and hemicellulase are mixed in a weight ratio of 1:2:1.5 instead of using 5 g of the mixed enzyme, including cellulase, beta-glucanase, hemicellulase, arabinase and xylanase. A camphor extract was obtained in the same manner as in Example 1-2, except that 5 g of biscozyme L was used.

Example 1-5

Except for using a mixed extraction solvent in which ethanol, methanol and water are mixed in a weight ratio of 1: 1.5: 0.5, and a mixed extraction solvent in which ethanol, methanol and water is mixed in a weight ratio of 1: 0.5: 1 is used, the above In the same manner as in Example 1-2, camphor extract was obtained.

Test Example 1: Antioxidant effect measurement (DPPH assay)

1,1-diphenyl-2-picrylhydrazyl radical (DPPH, 1,1-diphenyl-2-picrylhydrazyl radical), which contains relatively stable free radicals and is known to exhibit strong absorption properties in the magenta region of 517 nm. Using the widely known antioxidant screening method, the antioxidant effect of the camphor extract obtained in Examples 1-1 to 1-5 was measured. The final concentrations of camphor extracts obtained in Examples 1-1 to 1-5 were each 200 μg/mL, reacted at DPPH 60 μM for 30 minutes, and absorbance was measured at 517 nm to calculate antioxidant activity.

As a result, the antioxidant activity of the camphor tree extract obtained in Example 1-2 was excellently measured as 93%, whereas the camphor tree extract obtained in Examples 1-1, 1-3, 1-4, and 1-5 were respectively It was confirmed that 84%, 72%, 81%, and 83% showed relatively low antioxidant activity compared to the camphor extract obtained in Example 2.

Test Example 2: Observation of melanocyte migration promoting effect

For melanocyte migration, normal melanocytes cultured in advance were used, and a microscope capable of taking video was used. Five cultured cell samples were treated with camphor extracts obtained in Examples 1-1 to 1-5, respectively, to perform cell migration experiments at non-toxic concentrations. 50,000 cells were applied per well using a special incubator that cells could not adhere to the center of the well. Initially, there were no cells in the center, but the effect of cell migration was determined according to how much the cells moved to the center over time. After 3 hours after the cells were applied, 100 μL of a culture solution to which the material was added twice was added. After the culture solution was discarded every 2 days, it was replaced with a medium to which camphor extract was added. The material was treated for 10 days and photographed with the Nano Entec's JuLi Stage ^TM device. The last day (10th day) was taken by treatment with 5 μM of calcein AM.

As a result, in the samples cultured with the culture solution to which the camphor extract obtained in Examples 1-1, 1-3, 1-4, and 1-5 was added, the cells in the center were increased by 2.3 times, 1.6 times, 3.28 and 3.12 times, respectively. On the other hand, it was confirmed that the sample treated with the culture solution to which camphor extract obtained in Example 1-2 was added for a week increased the number of cells in the center by 6.02 times, and the effect of promoting cell migration was relatively greatly improved.

Among them, changes in the number of cells were observed before and after treatment for a week with the culture solution to which camphor extract obtained in Examples 1-2 and 1-3 respectively was added, and the results are shown in FIGS. 1A and 1B. 1A and 1B, FIG. 1A shows the change in the number of cells before and after treatment for a week with the culture solution to which the camphor extract obtained in Example 1-3 was added. It was confirmed that the number of cells in the center increased by 3.28 times after treatment (day 7) compared to before treatment (day 0). 1B shows the change in the number of cells before and after treatment for a week with the culture solution to which the camphor extract obtained in Example 1-2 was added. It was confirmed that the number of cells in the center was increased by 6 times after treatment compared to before treatment.

Examples 2-1 to 2-5: Preparation of skin external preparations for promoting melanocyte migration

A creamy emulsion was prepared with the composition shown in Table 3 below. The aqueous phase component and the oil phase component were each heated to 75° C., and the oil phase component was mixed with the aqueous phase component. Then, after cooling to 40°C by adding a neutralizing agent, a flavoring agent and camphor extract obtained in Examples 1 to 5 were added to prepare a creamy emulsion containing camphor extract.

Ingredient name content(%) Purified water 80.09 Hydrolyzed Hyaluronic Acid 0.03 Dipotassium glycyrrhizate 0.03 1,2-hexanediol 2 glycerin 5 Xanthan gum 0.15 Cetearylolibate One Sorbitanolivate One Caprylic Acid 0.5 Capric Triglyceride 0.5 Jojoba seed oil 0.5 Caprylylmethicone One Camphor leaf extract 5 Propanediol 2 Ethylhexylolibate 0.4 Sodium acrylate copolymer 0.4 Polyglyceryl-4 oleate 0.4 Sum 100

Test example 4: Checking the efficacy of external preparations for skin containing camphor extract

The skin external preparations prepared in Examples 2-1 to 2-5 were applied twice a day in the morning and evening for 6 months to two subjects. In particular, excellent recovery of skin color was confirmed in both patients who applied the external preparation for skin containing camphor extract obtained in Example 2 (FIG. 2). This can be said to be a result showing that camphor extract can be used as a treatment aid for pigment loss disorders in which skin color is lost.

Claims (15)

Functional cosmetic composition for improving pigment loss diseases comprising camphor extract or a fraction thereof as an active ingredient. The functional cosmetic composition according to claim 1, wherein the extract or a fraction thereof promotes the migration of melanocytes. The functional cosmetic composition according to claim 2, wherein the camphor extract or fraction thereof is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The functional cosmetic composition according to claim 3, wherein the functional cosmetic composition has one formulation selected from lotion, emulsion, cream, pack, essence, gel, powder, foundation, detergent, lotion, and essence. The functional cosmetic composition according to claim 4, wherein the camphor extract is extracted with at least one extraction solvent selected from water and C ₁ to C ₄ alcohol. The functional cosmetic composition of claim 5, wherein the C ₁ to C ₄ alcohol is selected from methanol, ethanol, or a mixed alcohol thereof. The functional cosmetic composition according to any one of claims 1 to 6, wherein the camphor tree extract is extracted by an extraction method comprising the following steps:
(A) ultrasonically treating camphor powder,
(B) performing an enzymatic decomposition reaction of the sonicated camphor powder with a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 1.5 to 2.5: 1 to 2,
(C) extracting the camphor powder subjected to the enzymatic decomposition reaction with a mixed extraction solvent in which ethanol, methanol, and water are mixed in a weight ratio of 1: 1 to 2: 0.25 to 0.75. A quasi-drug composition for treating pigment loss diseases comprising camphor extract or a fraction thereof as an active ingredient. The quasi-drug composition according to claim 8, wherein the extract or a fraction thereof promotes melanocyte migration. The quasi-drug composition of claim 9, wherein the camphor extract or a fraction thereof is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The quasi-drug composition of claim 10, wherein the quasi-drug composition has the form of an external preparation for skin. The quasi-drug composition of claim 11, wherein the external preparation for skin has one formulation selected from ointments, patches, gels, creams, and sprays. The quasi-drug composition of claim 12, wherein the camphor tree extract is extracted with at least one extraction solvent selected from water and C ₁ to C ₄ alcohol. The quasi-drug composition of claim 13, wherein the C ₁ to C ₄ alcohol is selected from methanol, ethanol, or a mixed alcohol thereof. The quasi-drug composition according to any one of claims 8 to 14, wherein the camphor tree extract is extracted by an extraction method comprising the following steps:
(A) ultrasonically treating camphor powder,
(B) performing an enzymatic decomposition reaction of the sonicated camphor powder with a mixed enzyme in which cellulase, beta-glucanase, and hemicellulase are mixed in a weight ratio of 1: 1.5 to 2.5: 1 to 2,
(C) extracting the camphor powder subjected to the enzymatic decomposition reaction with a mixed extraction solvent in which ethanol, methanol, and water are mixed in a weight ratio of 1: 1 to 2: 0.25 to 0.75.

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