KR20140096738A

KR20140096738A - Soup comprising the extract of cinamomum camphora siebi and skin lightning agent

Info

Publication number: KR20140096738A
Application number: KR1020130009708A
Authority: KR
Inventors: 전풍규
Original assignee: 전풍규
Priority date: 2013-01-29
Filing date: 2013-01-29
Publication date: 2014-08-06

Abstract

The invention relates to a soup using an extract of camphor trees, which is manufactured by adding 10 L of 50 wt% ethanol solution to 1 kg of dried and pulverized turning hays of camphor trees, dipping the dried and pulverized turning hays of camphor trees for seven days at room temperature, filtering an extract, collecting the extract, removing solvents, performing drying, and mixing 0.1-50 wt% of the extract of camphor trees extracted as powder with a particle size of 0.3-300 μm. When using camphor trees, the camphor trees can be used by being pulverized and the extract can be used as well. Even though extraction can be performed using any part of camphor trees, such as stems, branches, leaves, flowers, seeds, barks, sap, roots, sprouts, and the like, in order to conveniently produce the extract, leaves, stems, and seeds are preferable. In addition, even though raw leaves and stems are used for extraction, it is desirable that extraction is performed after treatment such as cutting, drying, pulverizing, and the like in order to increase extraction efficiency. Extraction can be performed by dipping in an extraction solvent or an extraction method using supercritical fluids or subcritical fluids.

Description

FIELD OF THE INVENTION [0001] The present invention relates to soaps and skin lightening agents containing camphor extract,

The present invention relates to a skin external preparation for whitening, which is effective for inhibiting the production of melanin by mixing Cinaanomomum camphora Sieb extract and for preventing and improving pigmentation after sunshine, freckles and the like.

Soaps containing natural ingredients, natural mineral powders and their extracts have been developed to solve the disadvantages of soaps made from various chemical ingredients that cause skin irritation. Recently, research on whitening agents as cosmetics has become increasingly necessary as skin blackness is recognized as skin aging due to ultraviolet rays, along with an improvement in the living standard of Asian countries, which prefer emotionally white skin. On the other hand, in order to develop a whitening cosmetic product which is relatively less toxic and is natural-friendly, researches are being conducted to search for and use herbal medicine having whitening action. For example, Korean Patent Publication No. 1991-8660 proposes a whitening cosmetic using a mulberry extract, and Korean Patent Publication No. 1993-28783 discloses a whitening composition containing a mulberry extract.

It is reported that melanin pigment is formed due to abnormal stimulation of ultraviolet rays from hormone abnormality or sunlight, and it is caused by abnormal deposition in the skin. The melanin pigment, which is the cause of skin pigmentation, is produced in the melanin-producing granules in the melanocytes (melanocytes) between the epidermis and the dermis, and the melanin produced diffuses into adjacent cells by penetration. The biochemical reaction in this melanocyte is presumed to be as follows. That is, tyrosine, which is an essential amino acid, is converted to dopaquinone by the action of enzyme tyrosinase, and it is a process of producing melanin pigment by the enzymatic or non-enzymatic oxidation to change the melanin to black through a red pigment and a colorless pigment . Therefore, inhibiting the action of tyrosinase, the first step of the reaction, is important in inhibiting melanogenesis.

However, the compound that inhibits the tyrosinase action is not so effective as improving the skin pigmentation because the effect of the compound is very gentle except for hydroquinone. On the other hand, the effect of hydroquinone is once acknowledged, but its use is limited in general because of its sensitivity. In order to improve the safety, experiments using monoester and alkyl monoether, which are higher fatty acids, have been carried out. However, since esters are decomposed by hydrolytic enzymes in the body, they are not necessarily safe.

The inventors of the present invention have found that camphor tree extracts grown in Jeju Island in Korea have a melanin formation inhibitory action and a tyrosinase inhibitory action in order to solve these problems, and thus the present invention has been completed.

In the present invention, 10 liters of a 50% by weight ethanol aqueous solution is added to 50 to 99.9% by weight of a soap stock solution and 1 kg of dried and ground powder of camphor tree, and the mixture is immersed at room temperature for 7 days. The extract solution is recovered by filtration, And then dried again to obtain a mixture of 0.1 to 50% by weight of a camphor extract extracted with a powder having a particle size of 0.3 to 300 탆.

The present invention also relates to a skin whitening agent comprising as an active ingredient a camphor extract obtained by extracting 9 kg of water with 1 kg of dried ground powder of camphor plant, refluxing at 90 캜 for 6 hours, recovering the extract by filtration and removing the solvent .

In addition, the present invention is characterized in that 9 l of methanol is added to 1 kg of dried camphor plant of camphor plant, dipped at room temperature for 7 days, recovered by filtration, removed solvent, and obtained camphor extract as an active ingredient.

The whitening external preparation for skin of the present invention has melanin formation inhibitory action and tyrosinase activity inhibiting action, and thus it has been found that it has excellent effect on skin whitening by reducing coloring, deposition, freckle and stain after sunlight.

When camphor wood is used, it may be pulverized as it is, but an extract may be used. Extraction can be done by using any part such as camphor stem, branch, leaf, flower, seed, bark, sap, root, stem, or sprout, but for easy use, it is better to use leaf, stem and seed. Leaves and stems can be used. When extracted, it may be used as raw material. However, it is preferable to extract after the treatment such as sloughing, drying, and pulverizing, considering the extraction efficiency. Extraction or extraction solvent, or an extraction method using supercritical fluid and subcritical fluid. In order to increase the extraction efficiency, it was possible to homogenize in stirring and extraction solvent. The extraction temperature is preferably about 5 캜 to a temperature below the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and extraction temperature, but it is suitably about 1 hour to 14 days.

In addition to water, it is possible to use, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, , Esters such as ethyl acetate, and ketones such as acetone and ethyl methyl ketone. These solvents may be used alone or in combination of two or more. In addition, saline, phosphate buffer solution, phosphate buffered saline solution or the like may be used. Further, one or two or more subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia, and the like may be used.

The above solvent extract of camphor can be used as it is, but it can be used by dissolving it in water or a polar solvent again. Concentration and dryness can be used by refining such as decolorization, deodorization, desalting and the like, May be used after the fractionation treatment. The camphor tree extract, its processed product and its fraction may be lyophilized after each treatment and fractionation, and dissolved in a solvent during use.

The camphor extract has excellent moisturizing, cell activating, whitening and antioxidant functions and can be used as a moisturizer, cell activator, whitening agent, antioxidant, external skin preparation and food. In addition, moisturizers, cell activators, whitening agents, and antioxidants containing camphor extract as an active ingredient can be used for hair as well as for topical application to the skin, orally ingested, and can be applied to foods, beverages and medicines.

Moisturizing agent with camphor extract as an active ingredient exerts excellent moisturizing effect on skin and hair and has high moisturizing effect especially on skin.

The cell activator containing the camphor extract as an active ingredient exhibits an excellent effect on various cells, but exerts an excellent effect on dermis fibroblasts.

The whitening agent containing the camphor extract as an active ingredient exerts an effect on improving symptoms of pigmentation such as stain and freckles, and exerts an excellent effect on the inhibition of the production of melanin, in particular, due to inhibition of tyrosinase activity.

An antioxidant containing camphor extract as an active ingredient exhibits excellent antioxidative activity, but exerts excellent effects especially on free radical scavenging action.

It is also possible to obtain an external preparation for skin exhibiting excellent effects in preventing and improving skin symptoms such as wrinkles, dark circles, skin elasticity, wrinkles, dullness, dryness and wrinkles by blending the camphor extract with an external preparation for skin, It can also be used as a skin external preparation for skin whitening. The camphor extract can also be used in the same food and beverage for cosmetic, health maintenance, or nutritional purposes.

The blending amount of the camphor extract in the external preparation for skin can be adjusted depending on the kind of the external preparation for skin and the purpose of use, but is preferably from 0.0001 to 50.0% by weight, more preferably from 0.0001 to 50.0% by weight, 0.001 to 25.0% by weight.

The formulation of the external preparation for skin containing the camphor extract is optional and can be provided, for example, as a solubilization system such as lotion, an emulsion system such as cream or lotion, or a dispersion system such as caramel lotion. It may also be provided in various formulations such as aerosols, ointments, powders, granules, etc., which are filled with a spraying agent.

In addition to the camphor extract, the external preparation for skin that contains the camphor extract may contain at least one component selected from the group consisting of general medicines, quasi drugs, skin cosmetics, hair cosmetics, oily ingredients, moisturizing agents, powders, colorants, emulsifiers, solubilizers, , A thickener, a medicine, a perfume, a resin, an antibacterial antiseptic, an alcohol, and the like. In addition, other cell activators, whitening agents, and antioxidants can be used in combination within the range not impairing the effects of the present invention.

<< Examples >>

Hereinafter, a production example of camphor extract, a test for evaluating each action, an external preparation for skin, a prescription example for food, and a use test will be described in detail. However, the technical scope of the present invention is not limited to the following examples.

[Production Example 1]

10 kg of a 50% by weight aqueous ethanol solution was added to 1 kg of dried crumbs of camellia whole plants, and the mixture was immersed at room temperature for 7 days. The extract was collected by filtration, and the solvent was removed to obtain a camphor tree extract.

[Production Example 2]

9 kg of water was added to 1 kg of dried ground powder of camphor plant, and the mixture was refluxed at 90 ° C for 6 hours. The extract was collected by filtration, and the solvent was removed to obtain a camphor tree extract.

[Production Example 3]

9 kg of methanol was added to 1 kg of the dried ground powder of camphor plant, and the mixture was immersed at room temperature for 7 days. The extract was collected by filtration, and the solvent was removed to obtain a camphor tree extract.

[Production Example 4]

The camphor plant was added to the supercritical extraction system and extracted with a supercritical fluid of carbon dioxide at a pressure of 15 MPa at 40 ° C. The extract was recovered and a camphor tree extract was obtained.

First, the resurfacing action of the dermis fibroblast of the camphor extract is evaluated. The samples were evaluated as samples 1 and 2, respectively, in which a mixture of camphor leaf and stem was extracted by Preparation Example 1 and Production Example 4, respectively.

The evaluation was done as follows. Normal human dermal fibroblasts were seeded on a 96-well microplate to 2.0 × 10 4 cells / well. The seeding badge is Dulbecco? Modified Eagle Medium (DMEM) supplemented with 1% fetal bovine serum was used. After culturing for 24 hours, the medium was replaced with a test medium containing a sample of a desired concentration and cultured for 48 hours. Then, the medium was exchanged with a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazole) -2,5-diphenyltetrazolium bromide (MTT) and cultured for 2 hours. The formazan was extracted from 2-propanol and the absorbance at 550 nm was measured in a microplate reader. At the same time, the absorbance at 650 nm was measured with turbidity and the cell activation was evaluated by the difference of the two measured values. The evaluation results are shown in Table 1 as a relative value obtained by adding 100 as the cell activating activity of the sample-free blank. Also, * and ** in the table show the significance probability less than 1% (P <0.01) ** in the significance probability less than 5% (P <0.05) and * in the t test.

Sample 1
Addition concentration (mg / mL) Dermal fibroblast
Resurrection Sample 2
Addition concentration (mg / mL) Dermal fibroblast
Resurrection Blank 100 Blank 100 0.25 142 ** 0.25 105 * 0.5 170 ** 0.5 110 ** One 180 ** One 120 **

As can be seen from Table 1, considerable dermal fibroblast activating action was observed in media supplemented with camphor tree extract. In particular, when 0.25 ~ 1.0 mg / mL of sample 1 and 0.5 ~ 1.0 mg / mL of sample 2 were added, significant dermal fibroblast activating action was recognized at less than 1% risk factor as compared with blank. For this reason, it has been found that camphor tree extract has excellent dermal fibroblast activating action.

The following shows the evaluation of the inhibitory effect of camphor extract on melanin production. The samples were obtained by extracting a mixture of camphor leaves and stems according to Production Example 1 and Production Example 4 using Samples 1 and 2, respectively.

The evaluation was done as follows. B16 mouse melanoma F0 strain (B16F0) cells were sown on a 35 mm plate at 2,000 per dish. After 24 hours of culture, the medium was replaced with DMEM supplemented with fetal serum (FCS) supplemented with 5% by weight or more of a sample of a desired concentration. After 7 days of culture, the cells were recovered using 0.25% trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell suspension. Further, in place of the medium to which the sample was added, 5% by weight or more fetal bovine serum (FCS) was added to the DMEM medium. In addition, the visual evaluation was carried out in five stages as shown in Table 2. Table 3 shows the determination results.

Judgment evaluation One It does not blacken at all 2 Very black 3 Darken 4 It gets pretty black. 5 Remarkably black

Sample 1
Addition concentration (mg / mL) Melanin occurrence
Inhibitory action Sample 2
Addition concentration (mg / mL) Melanin occurrence
Inhibitory action Blank 5 Blank 5 0.01 2 0.05 2 0.1 2 One 2

As can be seen from Table 3, in the case of using medium containing 0.01-0.1 mg / mL of sample 1 and 0.05-1.0 μg / mL of sample 2, blackening does not occur in most cases, and the camphor extract has excellent melanin production inhibitory effect It turned out.

Next, a prescription example of a skin external preparation containing a camphor tree extract according to the present invention is shown.

[Formulation Example 1]

(1) Squalene 10.0 (% by weight)

(2) Methylphenylpolysiloxane 4.0

(3) hydrogenated palm kernel oil 0.5

(4) hydrogenated soybean phospholipid 0.1

(5) Polyoxyethylene monostearate

Sorbitan (20EO) 1.3

(6) Sorbitan monostearate 1.0

(7) glycerin 4.0

(8) Palladium oxybenzoate 0.1

(9) Carboxyvinyl polymer 0.15

(10) purified water 53.85

(11) Arginine (1 wt% aqueous solution) 20.0

(12) camphor extract [Preparation Example 1] 5.0

Preparation: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. Meanwhile, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil component is added thereto with stirring, and homogeneously emulsified by a homogenizer. After completion of emulsification, start cooling (11) and (12).

[Formulation Example 2] Lotion

(1) Ethanol 15.0 (% by weight)

(2) Polyoxyethylene (40E.O.) Hardened castor oil 0.3

(3) fragrance 0.1

(4) Purified water 78.38

(5) Citric acid 0.02

(6) Sodium citrate 0.1

(7) glycerin 1.0

(8) Hydroxyethylcellulose 0.1

(9) camphor extract [Preparation Example 3] 5.0

Preparation: (1) Dissolve (2) and (3). After dissolving, (4) to (8) are added sequentially, and sufficient stirring (9) is added and uniformly mixed.

[Formulation Example 3] Cream

(1) Squalene 10.0 (% by weight)

(2) stearic acid 2.0

(3) hydrogenated palm kernel oil 0.5

(4) hydrogenated soybean phospholipid 0.1

(5) Sethanol 3.6

(6) glycerin monostearate of pro-type 2.0

(7) glycerin 10.0

(8) Pallooxine benzoate 0.1

(9) Arginine (20% by weight aqueous solution) 15.0

(10) Purified water 40.7

(11) Carboxyvinyl polymer (1 wt% aqueous solution) 15.0

(12) Camphor Extract [Preparation 1] 1.0

Preparation: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. Meanwhile, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The above-mentioned oil phase component is further homogenized and emulsified uniformly with stirring. After completion of the emulsification, further cooling is started at (11) and (12) is further added at 40 ° C and mixed uniformly.

[Formulation Example 4] Soap foam

(1) Stearic acid 16.0 (% by weight)

(2) Milistin acid 16.0

(3) glycerin monostearate 2.0

(4) Glycerin 20.0

(5) Sodium hydroxide 7.5

(6) Palm oil fatty acid amide propyl betaine 1.0

(7) purified water 36.5

(8) Camphor tree extract [Preparation Example 3] 1.0

Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C and mixed and stirred uniformly with the oil phase components. Start cooling and add (8) at 40 ° C and mix uniformly.

[Formulation Example 5] Bath salts

(1) Flavor 0.3 (% by weight)

(2) Camphor tree extract [Preparation Example 1] 1.0

(3) Sodium bicarbonate 50.0

(4) Sodium sulfate 48.7

Manufacturing method: Mix (1) to (4) uniformly.

The use test was carried out using the formulation containing the following camphor extract and the improvement effect on dermatitis due to drying was evaluated. At that time, each of the camphor extracts listed in Table 4 was contained in each of the emulsion prescriptions of Formulation Example 1, and the use tests were conducted in Examples 1 to 3. Also, the use test of the camphor tree extract was carried out at the same time as the comparative example 1 in which the water was replaced with purified water.

Types of Camphor Manufacturing example Example 1 Cinaanmomum camphora Sieb Production Example 1 Example 2 Cinaanmomum camphora Sieb Production Example 2 Example 3 Cinaanmomum camphora Sieb Production Example 4

Twenty female panelists of dry skin of 30 ~ 50 generations, in which each sample dermatitis symptom was significantly recognized, were used as a flock and blinds were used for 1 week, and changes in skin condition before and after use were observed and evaluated. As an index of skin symptom, three criteria of "improvement", "somewhat improved" and "no change" were evaluated for drying dermatitis, and each evaluation was obtained in Table 5.

Item evaluation Comparative Example 1 Example 1 Example 2 Example 3 Improving One 15 13 14 Dry skin Somewhat improved 3 4 4 5 No change 16 One 3 One

As shown in Table 5, there was no improvement in panelists of 60% or more in the control group without camphor extract. However, in the case of the group using the camphor extract, improvement of clear dermatitis was recognized in more than 60% of the panelists. As a result, the camphor tree extract has been found to have excellent moisturizing effect.

The method of manufacturing camphor soap using the camphor extract of this effect is as follows.

Camphor powder having a particle size of 0.3 to 300 탆 is obtained by using the camphor extract obtained in Production Examples 1 to 4 and added in an amount of 0.1 to 50% by weight based on the total weight of the soap, and the auxiliary additives of the general raw materials of the remaining soap are mixed . The soap raw material may include a surfactant and a copper / vegetable oil.

Claims

10 liters of a 50% by weight ethanol aqueous solution was added to 50 to 99.9% by weight of the soap stock solution and 1 kg of dried and crushed powder of camphor tree, and the mixture was immersed at room temperature for 7 days. The extract solution was recovered by filtration, A soap containing a camphor extract, which is prepared by mixing 0.1 to 50% by weight of a camphor extract extracted with a powder having a particle size of 0.3 to 300 탆.

A skin whitening agent containing camphor extract as an active ingredient, which is obtained by adding 9 liters of water to 1 kg of dried ground powder of camphor plant, refluxing at 90 ° C for 6 hours, filtering the extract, recovering solvent, and removing solvent.

9kg of methanol was added to 1kg of dried crushed camphor plant, immersed at room temperature for 7 days, and the extract was filtered to remove the solvent, and the obtained camphor extract was used as a moisturizer.