JPS63139104A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic, capable of activating skin cells, accelerating turnover of aged skin, having remarkable effects on improvement of rough skin as well as keratin and exhibiting beautifying effects at the same time, by blending low-molecular weight chondroitin sulfate with vitamin E. CONSTITUTION:A skin cosmetic containing 0.05-2.0wt% at least one of chondroitin sulfate having 2,000-20,000, preferably 3,000-12,000mol.wt. and salt thereof and 0.01-2.0wt% at least one selected from vitamin E and derivatives thereof at 1:0.01-1 weight ratio of both. This cosmetic is capable of producing remarkable effects on prevention of the skin against aging and skin beautifying effects in which moistness, flexibility, elasticity and gloss an be imparted to the skin. The above-mentioned cosmetic is applied to skin cream, skin lotion, skin milk, skin pack, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention provides a method of combining at least one type of low molecular weight chondroitin sulfate and its salts with an average molecular weight of 2,000 to 20,000, and at least one type selected from vitamin E and its derivatives. This invention relates to a skin cosmetic with excellent skin aging prevention effects (improving rough skin, improving keratin, accelerating the turnover of the stratum corneum, beautifying skin, etc.).

(Prior Art) Aging skin is rough skin that is dry and lacks smoothness, exhibits peeling of corneocytes, has a high collagen/elastin ratio in connective tissue, and has many wrinkles.

In addition, in aging skin, the turnover of the stratum corneum is slow due to a decline in cell metabolism, and therefore, it is said that the turnover becomes faster when anti-aging effects are applied to the skin. Skin cosmetics have been proposed.

However, all of the skin cosmetics that have been proposed so far,
For example, although it may be possible to improve the skin surface by making the skin moist and smooth, it is difficult to obtain skin cosmetics that are excellent enough to actively prevent aging skin. there were.

In order to expect an anti-aging effect on the skin, even if it is an appropriate technical condition to incorporate ingredients that have skin cell activating effects or blood circulation promoting effects into skin cosmetics, it is not necessary to use only those technical factors. It does not exhibit the expected anti-aging effect.

That is, skin cells take in skin cell activation components and skin cell nutrients exuded from skin capillaries present in the dermal layer, and carry out normal cell metabolism.

However, in the dermis, components such as hyaluronic acid, termatan sulfate, chondroitin sulfate, heparan sulfate, etc. The matrix component layer of the dermis, or the epidermis, is composed of lymph fluid, lipids, acidic mucopolysaccharides, etc. that fill the spaces between basal cells, wire cells, granule cells, and corneocytes. Skin cell-activating components migrate through the mesenchymal layer and reach fibroblasts in the dermis, basal cells and wired cells in the epidermis, and only after the components are smoothly incorporated into each cell,
This results in promising anti-aging effects.

However, in aging skin, the epidermal cell layer becomes 1JjFI, and the amount of components such as lymph fluid and acidic mucopolysaccharides, which are components of the matrix and light-emitting substances in the epidermal cell layer, decreases, and as a result The skin cell activation components and nutrients exuded from the blood vessels cannot sufficiently migrate through the matrix component layer and the groove component layer, and the cells do not take up the components sufficiently, so the anti-aging effect is not expressed to the extent expected. .

It contains components that normalize the matrix components and bulk substances interposed between the skin capillaries and skin cells, and allow skin cell activation components, skin cell nutrients, etc. to be smoothly taken into the skin cells. There are still no proposals for skin cosmetics.

(Disclosure of the Invention) Therefore, based on the idea of soft soil, the present inventor has conducted intensive research on acidic mucopolysaccharides that contribute to normalization of substrate components and light-emitting substances. Sulfuric acid and its salts are polymers with a molecular weight of several hundred thousand, and are hardly absorbed through the skin, and the skin aging preventive effect aimed at by the present invention could not be obtained. 20000, preferably 3000~
A skin cosmetic containing at least one type of low molecular weight chondroitin sulfate and its salts of 12,000 and at least one type selected from vitamin E and its derivatives is,
The purpose of the present invention is to activate skin cells, accelerate the turnover of aging skin, and exhibit remarkable effects on improving rough skin and keratin. The present invention was completed after confirming that it also has a beautifying effect that imparts elasticity (tension) and gloss.

(Objective of the invention) That is, the object of the present invention is to improve rough skin, to improve keratin,
It is an object of the present invention to provide a skin cosmetic that has excellent skin aging prevention effects such as the effect of accelerating the turnover of the stratum corneum and the effect of beautifying the skin.

(Structure of the Invention) The present invention is characterized in that at least one type of low molecular weight chondroitin sulfate and its salts having an average molecular weight of 2,000 to 20,000 is blended with at least one type selected from vitamin E and its derivatives. It is a skin cosmetic.

(Specific explanation of composition) Chondroitin sulfate and its salts used in the present invention are known substances, and are a type of acidic mucopolysaccharide consisting of hexosamine, uronic acid, and sulfuric acid, and are used in cartilage, joints, eyes, skin, etc. It becomes a matrix component in the connective tissue of animals, and is widely distributed throughout the animal body in combination with proteins.

Chondroitin sulfate and its salts contain equal moles of N-acetylgalactosamine, uronic acid, and sulfuric acid, and are divided into three types of isomers A, B, and C depending on the bonding position of sulfuric acid, type of uronic acid, etc.
is known to exist. Purified chondroitin sulfate is also used industrially, and is a powder that is white or pale yellowish brown in color and has a slight odor and taste.It dissolves well in water but is difficult to dissolve in organic solvents such as alcohol and acetone. have a property.

The low molecular weight chondroitin sulfate and its salt used in the present invention can be obtained as follows.

Dissolve 5 g of commercially available sodium chondroitin sulfate with a molecular weight of 200,000 in 50 ml of water and pass through a resin column of Amberlite IR-120 (H type). The resulting effluent was heated to 75 ml in a hot bath with sufficient stirring. Warm at °C and neutralize with N-caustic soda. After cooling the solution to room temperature, 3 times the volume of ethanol was added, and the resulting precipitate was centrifuged, washed with ethanol, and dried to obtain sodium chondroitin sulfate powder. This sodium chondroitin sulfate is then treated with an acid to give chondroitin sulfate, which is then treated with another basic substance, e.g.
Chondroitin sulfate or salts such as chondroitin sulfate potassium, chondroitin sulfate triethanolamine, and chondroitin sulfate L-arginine were obtained.

In addition, in the above treatment, the average molecular weight of sodium chondroitin sulfate obtained by each treatment at a reaction temperature of 75°C and a heating time of 10 to 120 minutes is 2500 to 26000, respectively, and chondroitin sulfate and its salts with various molecular weights. was prepared and used in various tests of the present invention.

The average molecular weight of chondroitin sulfate obtained for each treatment time was measured using the Somogyi-Nelson method.

In addition, among acidic mucopolysaccharides, low-molecular-weight hyaluronic acid, dermatan sulfate, heparan sulfate, and their respective salts other than chondroitin sulfate were also tested for efficacy in the same manner as chondroitin sulfate, and the results showed that the objective of the present invention was No significant effect on skin aging was observed.

Furthermore, in order to further exert the skin aging prevention effects such as improving rough skin and beautifying the skin, which are the objectives of the present invention, we screened blood circulation promoters to be used in combination, and found that vitamin E and its derivatives are expected to be effective as the objectives of the present invention. showed the effect of

Vitamin E and its 8127i form used in the present invention are known substances, and depending on the number and position of the methyl group in the chroman nucleus, α-tocopherol, β-tocopherol, γ-tocopherol,
Tocopherol, δ-tocopherol, α-tocotrielle, β-tocotriole, r-)cotriole, δ-
There are eight types of isomers of tocotrielles, and each isomer has optical isomers. Although all of these isomers can be applied to the skin cosmetic composition of the present invention, DL-α-tocopherol is particularly preferred.

Further, as derivatives of vitamin E, tocopherol acetate, tocopherol succinate, tocopherol calcium succinate, tocopherol orotate, tocopherol nicotinate, etc. are known, and all of these can be used in the skin cosmetics of the present invention. I can do it.

As a result of various investigations into the blending ratio to maximize the synergistic effect of the above-mentioned constituent substances to be incorporated into skin cosmetics, the present inventor found that (1) low-molecular-weight chondroitin sulfate and its salts, (2) The ratio of the amounts of vitamin E and its derivatives (polymerization ratio) is (11: (21-1:0.0)
The ratio is preferably 1 to 1:1, and the excellent effects aimed at by the present invention were observed.

That is, the blood circulation promoter (2) promotes blood circulation in peripheral skin capillaries present in the dermis, activates the exudation of skin cell activating components and nutrients contained in the blood into the tissue, and further,
(1) improves the composition ratio of matrix components present in epidermal cells, thereby facilitating the uptake of substances such as skin cell activation components into skin cells, promoting skin metabolism, and (1) ) itself has a skin cell activation effect, and
(1)'s excellent curing ability contributes to the application and retention of an appropriate amount of moisture to the skin, resulting in the effect of producing moist, smooth, and firm skin.

In addition, low molecular weight chondroitin wt% of the skin cosmetic,
(2) should be 0.01 to 2.0 wt%, and if the amount is less than the lower limit of each amount, it will not be sufficient to achieve the desired effect of the present invention, while if it exceeds the upper limit, the increase will be commensurate with the increase. However, no improvement in the effects can be expected.

The skin cosmetics of the present invention are applicable to skin creams, skin lotions, skin milks, skin punctures, skin serums, and the like.

In addition to the above, pigments, fragrances, preservatives, surfactants, pigments, antioxidants, and the like may be appropriately incorporated into the skin cosmetic of the present invention within a range that achieves the object of the present invention.

(Examples) Hereinafter, the present invention will be described in detail based on Examples and Comparative Examples.

The rough skin improvement effect test, the stratum corneum improvement effect test, the stratum corneum turnover measurement test, and the sensory test (skin beautification effect test) are as follows.

(1) Effect test on improving rough skin The effect of continuous application for 4 weeks was investigated on 20 middle-aged and elderly subjects who had rough skin on their lower legs. Approximately 1 g of the sample was applied to the test site of the left lower leg of the subject once a day, and the condition of the skin before and after the test was judged according to the following criteria. No sample was applied to the right lower leg, which served as a control.

Judgment criteria for skin dryness - 2 Normal ±: Slight dryness, no flaking 12 Dryness, mild flaking ++: Moderate dryness, flaking 10 + 10: Dryness, significant flaking Compare the judgment results of the test site and control site before and after the test However, if the skin dryness has improved by two or more levels (e.g. +-)
-1+→-10) was considered valid, one level improvement was considered somewhat effective, and no change was considered invalid. The test results are shown as the number of subjects who found the drug to be effective or somewhat effective.

(2) Effect test on improving keratin (increasing the anti-peeling properties of keratinocytes) Scotch tape (Chihanmending tape) was adhered to the subject's skin before and after the above-mentioned rough skin improvement effect test, and when the tape was peeled off. The condition of the keratinocytes attached to the skin was examined in detail using a scanning electron microscope, and the skin keratinocyte anti-exfoliation property was analyzed according to the following criteria to determine the keratin improving effect.

Judgment criteria for keratin improving effect (increased anti-desquamation property of keratinocytes) Evaluation points: 1 No scale observed 2: Small scale scattered 3: Small to medium scale noticeable 4: Large scale noticeable If the difference between the evaluation score and that of the control site was 2 or more points, it was considered valid, if it was 1 point, it was considered somewhat effective, and if it was 0 points, it was considered invalid.

The judgment results are expressed as the number of subjects who found the test to be effective or somewhat effective.

(3) Test for measuring the turnover of the stratum corneum An ointment containing 5% by weight of the fluorescent dye Danzyl chloride in white petrolatum was prepared and applied to the skin of the forearms of 20 subjects for 24 hours. Osmotic binding of chloride. Thereafter, the test sample was applied to the same area twice a day (morning and evening), and the fluorescence of danzyl chloride was checked every day, and the number of days until the fluorescence disappeared was defined as the turnover of the stratum corneum of the skin.

The measurement results are shown as the average value of the number of days of the subjects. Note that the normal turnover of the stratum corneum of the skin is 14-16 days,
In aging skin, it lasts for about 18 days. On the other hand, if the anti-aging effect appears, the time will be shortened to around 12 days.

(4) Sensory test (skin beautification effect test) rough skin, fine wrinkles,
Samples were given to 20 female subjects (35 to 55 years old) complaining of dry skin, etc. twice a day (morning and evening) for three consecutive months, and then the effects were evaluated. The test results were shown by the number of people who answered that their skin was moist, smooth, or taut for each item of skin wettability, smoothness, and elasticity.

Comparative Examples 1 to 12, Examples 1 to 10 [Two-layer skin lotion] As shown in the composition below, the base of the two-layer skin lotion was chondroitin sulfate (hereinafter abbreviated as Chs), and the sodium salt of chondroitin sulfate was Chs-Na, Potassium salt is −,
L-arginine salt is abbreviated as 1) and vitamin E.
, tocopherol orotate (hereinafter abbreviated as OAT)
, tocopherol succinate (hereinafter abbreviated as KAT) were blended as shown in Table 1 to prepare each skin lotion, and the various tests described above were conducted. Furthermore, Hyaluron (1
) Composition (2) Preparation method (B) The oil phase component was added to the mixture and mixed uniformly, -
Add (q) and mix and disperse into the mixed aqueous phase components with stirring, and then fill the container. When using, shake and disperse the contents uniformly.

(3) Properties The results of various tests conducted on each two-layer skin lotion are listed in Table 1.

Examples 1 to 1 of the present invention compared to skin lotions in which chondroitin sulfate and one of its salts and vitamin E and one of its derivatives are not combined in Comparative Examples 1 to 12.
No. 10 skin cosmetics showed clearly good results in various tests.

It was clear that a skin cosmetic containing chondroitin sulfate and vitamin E had an excellent skin function enhancing effect.

Comparative Examples 13 to 16, Examples 11 to 16 [Skin cream] In the same manner as in Example 1, each skin cream was prepared with the following composition and various tests were conducted. The results are shown in the right column of Table 2. .

(2) Oil phase component obtained by dropwise mixing Preparation method 03+ component into component (5), and 0
In addition to (q), heat and melt the mixed aqueous phase components at 80°C, add the aqueous phase components into the oil phase component while stirring,
After emulsifying and mixing, each skin cream was prepared by cooling to 30°C while stirring.

(3) Properties Example 1, which is a skin cosmetic of the present invention, as shown in Table 2
It is clear that skin creams Nos. 1 to 16 are superior to Comparative Examples 13 to 16 in all properties, and they are also different in blending properties (effects of the invention). It is clear that Zero Hatsuho's skin cosmetics provide excellent skin cosmetics that enhance skin functions and exhibit remarkable effects on preventing skin aging.

Claims (1)

[Claims] A skin cosmetic comprising at least one type of low molecular weight chondroitin sulfate and its salts having an average molecular weight of 2,000 to 20,000, and at least one selected from vitamin E and its derivatives.