KR20040097589A - A cosmetic composition containing an extract of herbal medicines - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition containing the extract of herbal medicine consisting of Punica granatum Linne, Trichosantes Kirilowii Maxim, Cnidium officinale, Foeniculum vulgare Gaertner and Chinese angelica. The composition has excellent anti-aging and whitening effects on the skin by improving skin winkles, scavenging superoxide radical and inhibiting matrix metalloprotease-1 synthesis. CONSTITUTION: The herbal medicine consist of Punica granatum Linne, Trichosantes Kirilowii Maxim, Cnidium officinale, Foeniculum vulgare Gaertner and Chinese angelica in a weight ratio of 5-7:6-8:2:4:1. It is extract with at least one kind of extraction solvents from purified water, methanol, ethanol, glycerine, ethylacetate, butyleneglycol, propylene glycol, dichloromethane and hexane. The cosmetic composition contains 0.001-30.0 wt.% of the extract.

Description

A cosmetic composition containing an extract of herbal medicines

The present invention relates to a cosmetic composition containing the herbal herbal extract as a main active ingredient, and more specifically, the herbal medicine extracted by mixing and extracting the herbal medicines mainly used in herbal medicine, pomegranate, cheonhwabun, cheongung, fennel and white paper at an appropriate ratio. Compared with the anti-aging effect and skin whitening effect, superoxide radical scavenging effect, inhibition of collagen metalloprotease-1 synthesis, and excellent anti-aging effect and whitening effect, 0.001-30.0 wt% It relates to the cosmetic composition containing.

Human skin is constantly changing with age. Skin aging is largely divided into natural aging (or endogenous aging) and external aging (EB Doris, Cosmetics & Toiletories, 111, 31-37, 1996), and natural aging (endogenous aging) is artificial because it is influenced by genetic factors. While external control is difficult to control, artificial aging is relatively easy because external factors are affected by environmental factors. Representative external aging factors include ultraviolet rays, and the most prominent external aging phenomenon is wrinkle formation (HW Daniell, Ann Intern Med, 75, 873-880, 1971, GL Grove, J Am Acad Dermatol, 21, 631-). 637, 1989, CE Griffiths et al, Arch Dermatol, 128, 347-351, 1992).

One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (D. Harman, J. Gerontol, 11, 298-301, 1956). Free radicals are known to cause breakdown of connective tissue formation such as skin collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein function, intercellular energy transfer, and metabolism related molecules through increased collagenase synthesis. (AF Kligman and RM Lavker, J. Cutan. Aging Cosmet. Dermatol. 1, 5-12, 1988). The involvement of free radicals in aging means that antioxidants or various chemical scavengers can be used to delay the aging process.

In order to prevent aging due to ultraviolet rays, the most common method is to apply a product containing sunscreen directly to the skin.However, in 1988, retinoic acid was reported to be effective in reducing skin roughness and fine wrinkles of aged skin (KS Weiss). et al, JAMA, 259, 527-532, 1988), studies are being actively conducted to develop substances that inhibit or improve skin aging worldwide. Among them, vitamin derivatives such as vitamins A, C, and E and alpha -hydroxy acid (AHA) are representative substances of aging skin improvement known to date (Hermitte, Cosmetics & Toiletries, 107, 63-67, 1992, DR Rosenthal et al, J. Invest.Drmatol., 95, 510-515, 1990, TDDitre et al, J. Invest.Drmatol, 34, 187-195, 1996).

However, they are very unstable in the natural environment, so there is a problem in use, or somewhat insufficient to show a visible effect.

Factors that determine skin color include basically areas such as tanning due to blemishes, freckles and sun exposure, or overall pigmentation, other than acne, scars, and the distribution of keratin, Blood circulation, stress, and health. Among the above factors, the most important factor is pigmentation.

The pigments that affect skin color include melanin, melazoids, carotene, and hemoglobin, the most important of which is melanin, which is the most important factor affecting biosynthesis. Melanin plays an important role in absorbing or scattering ultraviolet rays to prevent damage to the skin from ultraviolet rays. There is no special maximum absorption wavelength and it absorbs light in all areas. In addition, it is excellent in removing the active oxygen species, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows the properties of free radicals. Pray.

The biosynthesis of melanin is a series of oxidation processes, starting with the conversion of tyrosine, an amino acid, from the melanocytes of melanocytes to tyrocinase and dihydroxy phenylalanine, followed by brown (pheomelanin) and black (eumelanin). It is formed of a polymer of). This biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the end of dendritic processes, and into the cytoplasm by the phagocytosis of keratinocytes. Accumulate around the nucleus of the site.

The synthesis of melanin, the number of melanosomes, and the movement to surrounding keratinocytes are partially affected by hormones and ultraviolet rays, and primarily by genetic influences. In addition, cytokines (cytokine), copper ions, copper, zinc, iron and other metal ions involved in the expression of tyrosinase, melanin synthesis and transport, and interferons, prostaglandins, histamines, etc. are known to be involved. Journal, 25: 77-127, 1999).

Conventionally, raw materials such as kojic acid, hydroquinone and vitamin C have been added to cosmetics to prevent skin diseases such as skin blackening, blemishes and freckles. Functional raw materials that inhibit the activity of tyrosinase, an enzyme involved in the main stage, or reduce the melanin pigment produced.

Recently, many cosmetics using herbal medicines have been developed to reduce skin irritation caused by various chemicals. Various herbal herbal medicines are extracted by a predetermined method, and the function of the extract is increasing, and there are increasing cases of developing various functional cosmetics. For example, mung bean is known to clean the skin, and other ginseng extracts, extracts of situation mushrooms, etc. have been found to have its own anti-aging or whitening effects, and are being applied to various cosmetic products.

These herbal herbal medicines have less side effects on the skin, and as the consumer's response to cosmetic products using herbal herbal medicines increases recently, the development value of cosmetic herbal medicines is increasing. Therefore, the present inventors studied the possibility of applying to cosmetics mainly on various medicines known as herbal materials.

Pomegranate bark is the bark of the fruit, stem and root of the pomegranate (Punica granatum Linne). The leaf is large, obovate or long oval, acute or basal, 2 ~ 8cm long, hairless on both sides. Flowers bloom in May-June, flowers are red, and bisexually run 1-5 on the short flower tip of the branch. Fruits mature in September-October, rounded with berry, with calyx lobes at the end, 6-8cm in diameter, ripened with yellow or yellowish red, fleshy, and often with irregular shells. Used in edible, ornamental, industrial, medicinal, edible fruit, used as ornamental water and stem, and used in herbal medicine and folk medicine. (Kim Tae-jung, Korea Plant Resources III, Seoul National University Press, 1996).

Cinnabar is the skinned root of the Trichosanthes kirilowii Maximowicz or the Trichosanthes kirilowii Maximowicz var. Japonica Kitamura (Pak and Cucurbitaceae). Appearance is uneven pale yellow white columnar, 3 ~ 5cm in diameter, 5 ~ 10cm in length, often split vertically, with irregular yellowish brown tube bundle. It has no smell and tastes slightly bitter. Cutting surface pale yellow and slightly fibrous. When viewed in a magnifying glass, the cross section has a broad radial tissue and yellow spots or small eyelets. Antipyretic, expectorant, drainage disinfectant, jaundice, diabetes, stroke, or sprays used in infants' skin diseases (Hae-seok Han, Pharmacology, Dongmyeongsa, 1998).

Cnidium officinale Makino is an irregular lump, sometimes vertically split, 5-10 cm long, 3-5 cm in diameter. Steamed roots are hard with dark brown keratin. Dried root stock is light yellowish brown to grayish white and rough and slightly light. There are overlapping nodules, and there are bumps on the outside. It has an unusual strong smell, taste is spicy, bitter and sweet. It is used for various gynecological diseases such as cold syndrome, anemia and dysmenorrhea with the aim of blood, tonic, soothing and pain relief in oriental medicine (Han Dae-seok, herbal medicine, Dongmyeongsa, 1998).

Fennel is the fruit of Feneniculum vulgare Miller. Long columnar twins, 3-8 mm long, 1-3 mm wide, 1-3 mm wide, sometimes 2-10 mm long. The outer surface is greyish green to greyish yellow. Each string has five prominent lines, and the strings are close to each other. Looking at the cross section under the microscope, Bunyan's left and right ridges are much more prominent than the others, with one large loss between each ridge, and two on the side where the fruit is attached to the fruit disease. It has an unusual aroma and taste. It is used in fragrances, gugung and expectorants (Han Dae-seok, Herbal Medicine, Dongmyeongsa, 1998).

White paper is the root of Angelica dahurica Benth. Et Hook. And its variants. Many long roots split from short roots, usually fusiform, 10 ~ 25cm long, grayish brown ~ dark brown on the outside, some leaves on the head, and densely bumps. Roots have a lot of fine wrinkles and transversely upright traces of roots. It has an unusual smell and is slightly bitter. The perimeter of the cross section is grayish white with many gaps, and the center is dark brown. It is used in antipyretic, soothing, analgesic, antidote, Cheongsangbangpungtang, Ojeoksan, and Sokyeong Byeoltang (Han, Dae-seok, Pharmacology, Dongmyeongsa, 1998).

Examples of applying such pomegranate, cheonhwabun, fennel, cheongung, white paper, etc. as cosmetics are disclosed in Korean Patent Application Nos. 1995-21415, 01-07367, 03-43561, 95-21415, 00-57071, respectively. However, the pomegranate bark, nectarine, fennel, cheongung, and white paper extracts disclosed in this application have only been shown to be effective as single-sided herbal extracts for each of these herbal medicines. not.

It is an object of the present invention to provide an herbal extract and a method for extracting the same, which exhibits excellent anti-aging and whitening effects such as harmful active oxygen radical scavenging action, collagen degrading enzyme synthesis inhibitory effect, and skin anti-wrinkle effect.

Another object of the present invention is to provide a cosmetic composition comprising the herbal herbal extract as an active ingredient.

In order to achieve the above object, the inventors of the herbal medicines such as pomegranate skin, hwahwahwa, fennel, cheongung and white paper among the various herbal medicines more scientifically and tried to maximize the extraction efficiency of the active ingredients, the five herbal medicines The herbal extracts were prepared by adjusting the mixing ratio of herbal medicines with high efficiency, and the present invention was intended to provide a cosmetic composition that exhibits a predetermined functional effect without further irritation containing the extracts.

The present invention relates to a cosmetic composition containing a medicinal herb extract composed of a mixed herbal medicine of pomegranate, nectar, fennel, celery and white paper.

In addition, the present invention relates to a cosmetic composition containing a medicinal herb extract prepared by mixing herbal medicines such as pomegranate skin, nectarine, fennel, celery, and white paper at a weight ratio of 5-7: 6-8: 2: 4: 1. The mixing ratio of each herbal medicine was prepared by experimenting with extracts under various conditions, and the effects such as anti-aging and whitening were the most excellent under the above conditions.

In addition, the present invention is a cosmetic containing a herbal medicine extract containing the herbal extracts, characterized in that at least one solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as an extraction solvent To provide a composition. Preferably, the herbal medicine extract can be prepared using 40-95% ethanol solution as the extraction solvent.

In another aspect, the present invention provides a cosmetic composition, characterized in that the extract is a liquid obtained by cooling, heating, filtration at room temperature or an extract obtained by further evaporating or lyophilizing the solvent.

In addition, the present invention provides a cosmetic composition, characterized in that the content of the herbal herbal extract is 0.001 to 30.0% by weight based on the total cosmetic composition. If it is less than 0.001% by weight, there is almost no skin improvement effect, and if it is 30.0% by weight or more, the effect of increasing the content is minimal.

The present invention also provides a cosmetic composition, characterized in that the cosmetic composition is selected from a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type formulation. do. The cosmetic composition can be used for skin aging prevention and whitening, such as excellent skin wrinkle improvement effect, harmful active oxygen radical scavenging action, collagen degrading enzyme synthesis inhibitory effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative description of the present invention and the scope of the present invention is not limited to these Examples.

Example 1 Preparation of Botanical Herbal Extracts

Finely mix the dry pomegranate, fennel, fennel, celery and white paper at the weight ratio of 5-7: 6-8: 2: 4: 1, and 95% hot (V / V) to 10% by weight of this mixed herbal medicine. ) And refluxed three times with an aqueous ethanol solution for 5 hours, followed by chilling, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 캜 or lower. Abdominal herb extract was prepared using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol to contain 0.001 to 30.0 wt% of the vacuum concentrate and the lyophilisate.

Comparative Example 1 Preparation of Pomegranate Extract

After dipping into 10% by weight of dry pomegranate skin, hot 95% (V / V) ethanol aqueous solution was added, and the mixture was refluxed three times for 5 hours, cooled, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 캜 or lower. Pomegranate skin extract was prepared using at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol so that the vacuum concentrate and the lyophilisate contained 0.001 to 30.0% by weight.

Comparative Example 2: Preparation of Natural Flower Extract

The finely dried and dried chrysanthemum was extracted in the same manner as in Comparative Example 1 to prepare a chrysanthemum extract.

Comparative Example 3: Preparation of Fennel Extract

The fennel extract was prepared by extracting the dried and fennel in the same manner as in Comparative Example 1.

Comparative Example 4: Preparation of the Archery Extract

The shrunk and shrunk the zigzag was extracted in the same manner as in Comparative Example 1 to prepare a zirconia extract.

Comparative Example 5: White Paper Extract Preparation

The white paper was shredded and extracted in the same manner as in Comparative Example 1 to prepare a white paper extract.

Example 2 Experiment of Determining Hazardous Reactive Oxygen Radical Scavenging Effect

In this example, the harmful active oxygen radical scavenging effect of the herbal extracts of the herbal medicine obtained in Example 1 was measured by NBT method.

In humans, molecular oxygen is essential to sustain life, but the consumption of oxygen produces a small amount of free radicals in vivo, and its strong oxidation destroys cell membranes and cellular components. Since these harmful free radicals have a great effect on the damage and aging of skin cells, antioxidant active substances having harmful free radical scavenging action will have an important effect on the living body.

In order to measure the scavenging effect of harmful active oxygen radicals, the active oxygen produced by xanthine and xanthine oxidase is measured by NBT method to evaluate the effect of medicinal herbs extract from toxic free radicals. In other words, active oxygen produced by xanthine and xanthine oxidase reacts with Nitro Blue Tetrazolium (NBT) to measure the blue color produced by this at a wavelength of 560 nm. It measures by the following method.

1.In a Bayer bottle, add 2.4 ml of 50 mM Na ₂ CO ₃ buffer, 0.1 ml of 3 mM xanthine solution, 0.1 ml of 3 mM EDTA solution, 0.1 ml of 0.15% BSA solution and 0.1 ml of 0.75 mM NBT solution, respectively, and 0.1 ml of sample solution. After addition, it is left to stand at 25 degreeC for 10 minutes.

2. Add 0.1 ml of xanthine oxidase solution, stir and react for 20 minutes at 25 ° C.

3. Add 0.1 ml of 6 mM CuCl ₂ solution to stop the reaction and measure the absorbance St at 560 nm.

4. In blank test, absorbance Bt is measured by using distilled water instead of sample solution as above.

5. The blank of the sample solution and the blank test solution is operated in the same manner using distilled water instead of the enzyme to measure the absorbance So and Bo.

The harmful active oxygen radical scavenging effect was calculated by Equation 1, and the results are shown in Table 1.

Inhibition Rate (%) = 1- (St-So) / (Bt-Bo)] X 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme addition of sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

Sample Name Harmful free radical radical scavenging effect (%) Comparative Example 1 92 Comparative Example 2 60 Comparative Example 3 85 Comparative Example 4 60 Comparative Example 5 65 Example 1 95.6 Green Tea Extract 93.6 Vitamin E 87

As shown in Table 1, the herbal herbal extract extracted by mixing the five herbal medicines at an appropriate ratio was found to have a significantly higher toxic radical scavenging effect than the herbal extract. The effect was confirmed. In addition, the herbal herbal extracts were found to have similar or superior antioxidant power as compared to green tea extract and vitamin E, which have excellent antioxidant effects.

Example 3: Experiment to Inhibit Collagen Degrading Enzyme Synthesis

In order to observe the inhibitory effect of collagen degrading enzyme synthesis of the herbal extracts obtained in Example 1, the experiments were performed by directly treating human fibroblasts obtained from humans or purchased commercially.

In a 48-well plate, add DMEM (Dulbecco's Modified Eagle's Media) containing 10% fetal bovine serum and human fibroblasts (50000 cells / well) and incubate until 70-80% growth. It was. The culture medium was then removed and washed with phosphate buffer. After irradiating ultraviolet ray A (4.2J / cm 2), the herbal herbal extract and the herbal herbal extract were replaced with fetal bovine serum-free medium containing 0.1% concentration, respectively, and cultured for 1 day. The amount of collagen degrading enzyme (MMP-1) synthesized by fibroblasts was measured by immunoassay (Enzyme Linked Immunosorbent Assay) (Petersen M.J., et al., J. Invest. Dermatol., 99, 440-444, 1992).

The measurement method was first removed after coating the culture supernatant in a 96-hole plate incubator for 24 hours. 3% serum albumin was added to block binding of nonspecific proteins. The primary antibody was treated for 1 hour and 30 minutes by treating the primary antibody specific to the collagenase, and then reacted for an additional 1 hour and 30 minutes by treating the secondary antibody to which the chromophore was bound. Color development was added to color at room temperature, and 3N sodium hydroxide was added to stop color development. The color of the reaction color is yellow, depending on the intensity of the reaction. A yellowish 96-hole plate incubator was measured at 405 nm using an absorbance spectrometer, and the degree of inhibition of collagenase synthesis was calculated by Equation 2 below. At this time, the reaction absorbance of the cell culture solution without the extract was used as a control.

The results are shown in Table 2, but the single-sided extract was weakly inhibited collagenase synthetase synthesis, it can be seen that the mixed herbal extracts in the appropriate ratio of single-sided extracts excellent collagenase synthesis inhibitory effect.

Inhibition rate of collagen degrading enzyme synthesis (%) = [1- (Reaction absorbance of cell culture solution treated with herbal extracts / reaction absorbance of control)] X 100

Sample Name Inhibition rate of collagen degrading enzyme synthesis (%) Comparative Example 1 6 Comparative Example 2 10 Comparative Example 3 30 Comparative Example 4 11 Comparative Example 5 10 Example 1 50 Control 0

Example 4: Experiment to measure melanin production inhibitory effect using B16F1 melanocytes

This Example is to determine the whitening effect by the degree of inhibition of melanin production on B16F1 melanocytes in order to confirm the whitening effect of the herbal extracts obtained in Example 1.

B16F1 melanocytes used in this example are cell strains derived from mice, and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16F1 melanocytes used in this example were used after being sold from ATCC (American Type Culture Collection, Accession No .: 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanocytes was measured as follows. B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 x 10 ⁶ per well, and the cells were attached and then incubated for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was performed with a slight modification of the method of Rotan: Cancer Res. , 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melanin was extracted by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then the absorbance of melanin was measured at 405 nm with a microplate reader. Percent inhibition of production was measured. Melanin inhibition rate (%) of B16F1 melanocytes was calculated by the equation (3), IC ₅₀ value is the concentration of a substance that inhibits melanin production by 50%.

% Inhibition = [(A-B) / A] X 100

A: amount of melanin in wells without added sample

B: Melanin amount in the wells to which the sample was added

As shown in Table 3, the medicinal herb extract extracted by mixing the five medicinal herbs at an appropriate ratio showed that the melanin production inhibitory effect was very superior to that of the medicinal herb extract. Confirmed. In addition, the herbal extracts were found to have a similar or superior whitening effect than the conventional whitening agents arbutin and hydroquinone.

Sample Name Inhibitory effect of melanin synthesis (IC ₅₀ ) Example 1 0.03% Comparative Example 1 0.2% Comparative Example 2 0.3% Comparative Example 3 0.2% Comparative Example 4 0.06% Comparative Example 5 0.3% Hydroquinone 0.03% Arbutin 0.5% Cereal Extract 5% Soluble Licorice Extract 0.03%

Test Example 1 Skin Wrinkle Improvement Effect

In this test example, the cosmetic preparations containing the herbal extracts obtained in Example 1 were prepared (Examples 5, 6, and 7), and the effect of improving skin wrinkles was evaluated by performing comparative experiments with Comparative Example 6.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 4. First, the phase b) recorded in Table 4 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream (Examples 5 to 7, Comparative Example 6). For 20 experimenters (20-35 year old female), the creams prepared in Examples 5 to 7 were applied to the right side of the face and the creams prepared in Comparative Example 6 were applied twice a day for 2 consecutive months to the left side of the face. It was.

After completion of the experiment, the wrinkles around the skin before and after using the product for 2 months after the use of the wrinkles around the face of the silicone resin was taken with a replica (replica), which was compared with the state of the wrinkles around the eyes with a skin micro wrinkle measuring device and a skin image analyzer.

Table 5 compares the average eye wrinkle depth and wrinkle reduction rate of the experimenter using the creams prepared in Examples 5 to 7 with the experimenter using the cream of Comparative Example 6. As shown in Table 4, it can be seen that the skin fine wrinkles improvement effect is excellent in the facial periphery skin of the experimenter who applied the cream containing the herbal extract of the abdominal herb.

Raw material Example 5 Example 6 Example 7 Comparative Example 6 end Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl monostearic acid ester 2 2 2 2 I Botanical Herb Extract 10 One 0.1 - Propylene glycol 5 5 5 5 Purified water Quantity Quantity Quantity Quantity

Note) Unit: weight%

Product used Before use 2 months after use Remarks Example 7 0.203 0.168 Average fine wrinkle depth - 17.24% Wrinkle Reduction (%) Comparative Example 6 0.202 0.195 Average fine wrinkle depth - 3.47% Wrinkle Reduction (%)

n = 20, p <0.01

Test Example 2: Skin Whitening Effect

In this test example, the cosmetics of Examples 5, 6, 7 and Comparative Example 6 were evaluated by performing a skin whitening effect on humans.

For 20 experimenters (women aged 20-35 years), the creams prepared in Examples 5 to 7 on the right side of the face and the creams prepared in Comparative Example 6 on the left side of the face were applied twice a day for 2 consecutive months. Applied. After completion of the test, the skins of the application areas on both sides of the face were compared by using an image analyzer and a color difference meter. Table 6 compares the facial skin color of the experimenter using the cream prepared in Example 7 with the facial skin color of the experimenter using the cream made in Comparative Example 6.

As shown in Table 6, it can be seen that the whitening effect is excellent in the facial skin of the experimenter who applied the cream containing the herbal herbal extract.

Experiment No. Example 7 Comparative Example 6 Right skin color Left skin color One 2 3 2 2.5 3 3 1.5 2.5 4 1.5 2 5 2 3 6 2 2.5 7 3 3.5 8 2.5 3 9 2 3 10 2 3.5 11 1.5 2.5 12 2.5 3 13 1.5 2 14 2.5 3.5 15 2 2 16 2.5 3 17 3 3 18 2.5 3.5 19 2.5 3 20 2 3

Other examples are shown below. That is, the lotion, milky lotion and essence containing the herbal herbal extract obtained in Example 1 were prepared in Examples 8 to 10. The lotion, milky lotion and essence containing these herbal extracts showed excellent effects on skin improvement such as toxic radical free radical scavenging effect, collagen degrading enzyme synthesis effect, and skin wrinkle improvement effect.

Example 8 Preparation of Lotion Containing Herbal Herbal Extracts Obtained in Example 1

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. . 0.05 g of the herbal extracts obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a skin lotion having a skin improvement effect.

Example 9 Preparation of Latex Containing Herbal Herbal Extracts Obtained in Example 1

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the herbal extract of the herbal medicine obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 ° C. to dissolve it. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 10 Preparation of Cosmetic Solution Containing Botanical Herbal Extracts Obtained in Example 1

To 5 g of 95% ethanol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester 1 g of herbal extracts obtained in Example 1 and an appropriate amount of pigments were mixed to obtain a essence having a skin improvement effect.

As described above, the herbal herbal extract of pomegranate skin, nectarine, fennel, celery and white paper of the present invention has a superior harmful active oxygen radical scavenging effect and collagen degrading enzyme synthesis inhibitory effect and superior to each medicinal herb extract. It has an excellent anti-aging effect and whitening effect such as skin wrinkle improvement effect, and has shown the effect of relieving skin irritation caused by cosmetics with natural herbal herbal extract.

In addition, the present invention can provide a cosmetic composition such as a lotion, cream, milky lotion, pack, etc. containing the herbal herbal extract.

Claims (9)

A cosmetic composition containing a medicinal herb extract consisting of a mixture of pomegranate, fern, fennel, celery and white paper. According to claim 1, wherein the herbal medicine is pomegranate, cheonhwabun, fennel, cheongung and white paper cosmetic composition containing the herbal extract extract, characterized in that the weight ratio of 5 to 7: 6 to 8: 2: 2: 1: . The herbal medicine according to claim 1 or 2, wherein at least one solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane is used as the extraction solvent. Cosmetic composition containing an extract. The cosmetic composition according to claim 1 or 2, wherein the extract is obtained by cold condensation at room temperature, heat filtration, or liquid obtained by further concentrating the solvent under reduced pressure or lyophilization. The cosmetic composition containing the herbal herbal extract according to claim 1 or 2, wherein the content of the herbal herbal extract is 0.001 to 30.0% by weight based on the total lyophilized weight of the cosmetic composition. The cosmetic composition of claim 1 or 2, wherein the cosmetic composition is selected from a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type, and water-in-oil (W / O) type. Cosmetic composition containing the herbal herbal extract to. The cosmetic composition according to claim 3, wherein the extract is obtained by cold condensation at room temperature, heat filtration, or liquid obtained by further concentrating the solvent under reduced pressure or lyophilization. The cosmetic composition containing the herbal herbal extract according to claim 3, wherein the content of the herbal herbal extract is 0.001 to 30.0% by weight based on the total lyophilized weight of the cosmetic composition. According to claim 3, wherein the cosmetic composition is a herbal medicine extract, characterized in that selected from the lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type formulation Cosmetic composition containing a.