KR102133102B1 - Cosmetic composition comprising the extract from BSF larva and method of preparing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract in Dongae, which can be used for skin whitening, wrinkle improvement, anti-aging, etc. and a method for manufacturing the same.
The cosmetic composition of the present invention contains extracts in Dongae, which has a tyrosinase inhibition rate, an elastase inhibition rate, a collagenase inhibition rate, and antioxidant efficacy, and thus functional skin whitening, wrinkle improvement, anti-aging, etc. Can be used as

Description

Cosmetic composition comprising extract in Dongae et al. and its manufacturing method{Cosmetic composition comprising the extract from BSF larva and method of preparing the same}

The present invention relates to a cosmetic composition containing an extract in Dongae and the like, and more particularly, to a cosmetic composition containing the extract in Dongae and the like, which can be used for skin whitening, wrinkle improvement, anti-aging, etc., and a method for manufacturing the same.

The skin is the primary barrier that protects the human body from external physical and chemical stimuli and is an important organ that performs various functions such as body temperature regulation, sensory, secretion, excretion, and vitamin D formation. The skin undergoes deterioration of the function of the skin and a decrease in visual aesthetics over time, specifically, skin relaxation, senile pigmentation, skin elasticity, skin keratinization, and skin gloss reduction. Skin aging is largely due to genetic factors such as internal aging and sunlight.

It is classified into external aging according to environmental factors, and this external aging forms wrinkles of the skin, and examples of factors for forming wrinkles include reduction in biosynthesis of free radicals, ultraviolet rays, and collagen.

Aging of the general skin appears due to a decrease in the function of the epidermis and dermis. In particular, it appears as a decrease in moisture of the epidermis, a delay in keratinocyte detachment and a decrease in collagen and elastin of the dermis. In addition, cell membrane oxidation by free radicals generated from respiration and external stimulation also promotes skin aging.

Internal aging of the skin cannot be artificially controlled except for regulating the expression of the causative gene, but in the case of external aging, it is prevented and treated by moisturizing and promoting fibroblast activity, promoting collagen production and inhibiting collagen degrading enzymes, and antioxidant activity.

Or you can delay it. Accordingly, in recent years, interest in functional cosmetics containing a specific bioactive substance for the purpose of delaying skin aging, etc. has increased and many studies have been conducted.

Meanwhile, Hermetia illucens is an environmental cleansing insect, and it is a bio-defense to rapidly decompose these organic wastes while living in organic wastes (food waste, animal wastes, agricultural by-products, etc.) containing a large amount of bacteria, fungi, and viruses. It possesses a mechanism to express strong antibacterial substances. Currently, attempts have been made to use Dongae-eul as an organic antibiotic or to treat organic waste such as food waste. However, Dongae, which is mainly used for treating organic waste, has never been applied to functional cosmetics such as skin wrinkle improvement or anti-aging.

The present invention is to provide a functional cosmetic using larvae in Dongae.

The present invention is to provide extracts for cosmetic products such as skin care, which can be used for skin whitening, wrinkle improvement, anti-aging, and the like.

One aspect of the present invention for achieving the above object relates to a cosmetic that uses the extract as an active ingredient in Dongae.

In the present invention, the extract of Dongae et al. has tyrosinase inhibitory activity, elastase inhibitory activity, collagenase inhibitory activity and antioxidant efficacy.

The present invention relates to a cosmetic preparation method comprising the steps of preparing an extract in Dongae and the like and adding the extract to the cosmetic ingredient.

The cosmetic composition of the present invention contains extracts in Dongae having tyrosinase inhibition rate, elastase inhibition rate, collagenase inhibition rate, and antioxidant efficacy, so that it is functional cosmetic such as skin whitening, wrinkle improvement, anti-aging, etc. Can be used as

1 is a graph of the tyrosinase inhibition rate according to the extraction solvent and concentration of the extract of Dongae et al.
2 is a graph showing the elastase inhibition rate according to the extraction solvent and concentration of the extract in Dongae.
Figure 3 shows the collagenase (Collagenase) inhibition rate according to the extraction solvent and concentration of the extract in Dongae.
Figure 4 is a graph measuring the total antioxidant capacity (Total antioxidant capacity) according to the extraction solvent of Dongae, etc., Figure 5 is a graph measuring the DPPH radical scavenger activity (DPPH radical scavenger activity) according to the extraction solvent of the extract of Dongae etc. to be.

Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings. However, the scope of the present invention is not limited to the description or drawings of the following embodiments. That is, the terms used in this specification are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In addition, terms such as “comprises” or “have” described herein are intended to indicate that there are features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, or one or more thereof It should be understood that the above or other features or numbers, steps, actions, components, parts or combinations thereof are not excluded in advance.

The cosmetic composition of the present invention includes an extract as an active ingredient in Dongae.

The extract of Dongae et al. is extracted from the larva powder of Dongae et al. The larvae extract of Dongae lamp can be prepared by adding powder of each solvent 0.01g/mL~0.5g/mL to Dongae lamp, boiling, filtering for 10 minutes to 1 hour, and centrifuging the supernatant to freeze-dry. have.

The solvent may be distilled water or an aqueous acetic acid solution (for example, 30% acetic acid).

The cosmetic (composition) may include 10 μg/mL to 5000 μg/mL, preferably 50 μg/mL to 1000 μg/mL, of extract from Dongae.

The extract of Dongae et al. may have tyrosinase inhibitory activity, elastase inhibitory activity, collagenase inhibitory activity and antioxidant efficacy.

Tyrosinase is a redox enzyme found in hair, eyes, and skin that breaks down tyrosine and participates in the biosynthesis of melanin, a biological pigment. The extract of Dongae et al. shows a higher tyrosinase inhibition rate than the positive control, and thus can be used as a skin lightening agent.

Elastase is a protease that breaks down Elastin, which is responsible for skin elasticity, and Elastin is a protein fiber that provides elasticity to cellular tissues and is an important protein that makes up the connective tissue of the lower back, skin, and blood vessels. When Elastase is excessively secreted, it decomposes Elastin in the skin, causing wrinkles in the skin. The extract of Dongae et al. shows higher inhibition rate of Elastase than the positive control, so it can be used as a wrinkle improving agent.

Collagenase is an enzyme that breaks down collagen by promoting the hydrolysis of peptide bonds of collagen constituting the skin.As collagen decreases by collagenase as the aging of the skin tissue decreases, the skin elasticity weakens and wrinkles This will happen. The extract of Dongae et al. shows a higher rate of collagenase inhibition than the positive control and can be used as a wrinkle improving agent.

As the cause of skin aging, free radicals generated through ultraviolet rays and breathing are considered as the most important factors. Since the extract of Dongae et al. exhibits high antioxidant performance, it can be used as a skin aging prevention and retardant.

The cosmetic may include ingredients that are usually added. For example, the cosmetic composition may be any one selected from lotion, lotion, cream, essence, pack, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo and hair rinse by adding known ingredients, etc. It can be prepared in one dosage form.

When the formulation of the cosmetic (composition) is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or non-acidic esters of sorbitan and the like can be used.

Stabilizers, solubilizers, vitamins, pigments and fragrances, which are commonly known components, may be added to the cosmetic.

As an example, the cosmetic is ethanol 1.5% by weight, natural liquid 1.0% by weight, betaine 3.0% by weight, dieel panthenol 3.0% by weight, sebum-A 1.0% by weight, arbutin paste 2.0% by weight, anenosine 2.0% by weight, eco gum 0.03% by weight, glycerin 4.0% by weight, collagen 1.0% by weight and Dongae, etc. may contain 50㎍ / mL ~ 1000㎍ / mL of extract.

The method for manufacturing a cosmetic product containing an extract in Dongae lamp of the present invention includes a step of preparing an extract in Dongae lamp and adding the extract to the cosmetic ingredient.

The extract manufacturing step of Dongae et al. includes a powder preparation step, a heating step, a pure supernatant preparation step and a lyophilization step.

In the powder manufacturing step, microwaves may be irradiated to larvae of Dongae, dried, and then pulverized to prepare powders of Dongae.

In the powder manufacturing step, the microwave irradiation time can be appropriately adjusted according to the amount or dry state of Dongae. For example, microwave irradiation can be performed for 1 minute to 45 minutes.

The heating step is a step in which the powder is added to distilled water or acetic acid, which is an extraction solvent, and then heated. In the heating step, 0.01 g/mL to 0.5 g/mL, preferably 0.05 g/mL to 0.2 g/mL, of powder can be added to the solvent.

In the heating step, the distilled water and acetic acid may be used as extraction solvents, respectively. At this time, acetic acid may be used as an extraction solvent by mixing distilled water and acetic acid in a volume ratio of 70:30.

The heating step may be performed at 80 to 120°C, preferably 90 to 100°C for 10 minutes to 1 hour, preferably 20 minutes to 30 minutes.

In the pure supernatant production step, the heated extract solution is centrifuged to recover the supernatant, and the recovered supernatant is filtered through a filter.

The centrifugation may be performed at 1 to 10° C. at a speed of 2000 to 6000 rpm for 10 minutes to 1 hour, but is not limited thereto.

In the pure supernatant preparation step, the centrifuged supernatant can be filtered (0.45 μm filtration filter) to obtain an extract.

The lyophilization may lyophilize the extract for about 24 to 96 hours, preferably about 60 to 68 hours.

The present invention includes the step of adding the extract to the cosmetic ingredient in the freeze-dried Dongae.

The cosmetic component may be a component that is conventionally added to the cosmetic composition described above.

Hereinafter, the present invention will be described in more detail with reference to Examples, but these are merely for illustrating preferred embodiments of the present invention, and the Examples are not intended to limit the scope of the present invention.

Example

Preparation of extracts in Dongae

After drying the larvae in Dongae and microwave for 3 minutes, powder was used. 10 g of powdered Dongae etc. was added to 100 ml of 30% Acetic acid (AC) (Example 1) and Distilled water (DW) (Example 2), respectively, and boiled for 20 and 30 minutes, respectively, and then boiled at 14,000 rpm and 4°C for 30 minutes. It was centrifuged. After that, only the supernatant was filtered with a 0.45 μm syringe filter and lyophilized for 68 hours.

Functional analysis of extracts from Dongae

(1) Tyrosinase inhibition

Tyrosinase inhibition was measured with a Tyrosinase inhibitor Screening Kit (Biovision, CA) and Kojic acid 75uM was used as a positive control.

1 is a graph of the tyrosinase inhibition rate according to the extraction solvent and concentration of the extract of Dongae et al. Referring to Figure 1, the inhibition rate (inhibition rate) of Kojic acid used as a positive control was 37.51%. The treatments of Examples 1 and 2 showed that the tyrosinase inhibition rate increased with increasing concentration in Dongae. In addition, in the treatment groups of Example 2 (D.W 1000 ug/mL) and Example 1 (A.C 1000 ug/mL), tyrosinase inhibition rate higher than that of the positive control group can be confirmed.

(2) Elastase inhibition

Elastase was measured with a Molecular Probes EnzChek Elastase Assay Kit (Invitrogen, CA), and 5 uM, N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone was used as a positive control.

Figure 2 shows the elastase inhibition rate according to the extraction solvent and concentration of the extract in Dongae. 2, the elastase inhibition rate of the positive control was measured to be 52%. The treatments of Examples 1 and 2 showed that the elastase inhibition rate increased with increasing concentration in Dongae.

(3) Inhibition of collagenase

The amount of collagenase inhibition was measured using the method enclosed in the Collagenase Activity Colormetric assay kit (Biovision, CA), and Kouic acid 75uM was used as a positive control.

Figure 3 shows the collagenase (Collagenase) inhibition rate according to the extraction solvent and concentration of the extract in Dongae. Referring to FIG. 3, the inhibition rate of Kojic acid used as a positive control was measured to be 71.72%. The treatments of Examples 1 and 2 showed that collagenase inhibition rate increased with increasing concentration in Dongae. In addition, the treatment of Example 1 (A.C 1000 ug / mL) showed a higher collagenase inhibition rate than the positive control.

(4) Antioxidant efficacy

The antioxidant capacity of the sample was measured by Total Antioxidant capacity (TAC) and DPPH radical scavenging activity. The total antioxidant capacity assay measures the degree of reduction of Cu2+ to Cu+ by proteins such as small molecules such as ascorbate, uric acid, GSH, and vitamin E, or albumin and transferrin, which are present in the sample. antioxidant capacity). The total antioxidant capacity of A.C and D.W extracts in Dongae et al. was analyzed by purchasing Total Antioxidant Capacity Colormetric Assay Kit (Biovision, CA) according to the method provided by the manufacturer. After mixing 100 μL of Cu2+ working solution with 100 μL of extract (50 μg/mL, 100 μg/mL, 500 μg/mL, 1000 μg/mL) of each sample (Examples 1 and 2), block the light at room temperature and react for 90 minutes. Next, the absorbance value was measured at 570 nm. The total antioxidant capacity of the sample was expressed in terms of trolox equivalent capacity (nM TEC/mg sample) according to the standard curve prepared by reacting the standard substance trolox (water-soluble tocopherol) under the same conditions. α,α'-diphenyl-β-picrylhydrazine (DPPH) is a compound that contains stable free radicals and measures the degree of reduction by receiving electrons or hydrogen from the antioxidants in the sample, thereby measuring the DPPH radical scavenging activity of the sample Is to evaluate. To evaluate DPPH free radical scavenging activity of A.C and D.W extracts in Dongae et al., 100 μL of the sample and 50 μL of DPPH solution dissolved in methanol were mixed and reacted for 30 minutes by blocking light at room temperature. The concentration of the positive control tocopherol was prepared and used so that the final concentration in the reaction solution was 20 μM. After the reaction was completed, the absorbance was measured at 517 nm, and the DPPH radical scavenging activity was calculated using the following equation.

DPPH radical scavenging activity (%) = [1-(absorbance of sample addition/absorption of no sample)]× 100

Figure 4 is a graph measuring the total antioxidant capacity (Total antioxidant capacity) according to the extraction solvent of the extract of Dongae, etc., Figure 5 is a graph measuring the DPPH radical scavenger activity (DPPH radical scavenger activity) according to the extraction solvent of the extract of Dongae etc. to be.

4 and 5, the treatments of Examples 1 and 2 showed that the total antioxidant capacity and DPPH radical removal capacity increased with increasing concentration in Dongae.

In the above, preferred embodiments of the present invention have been described in detail, but these are for illustrative purposes only, and the protection scope of the present invention is not limited thereto.

Claims (5)

delete delete delete delete A method of manufacturing a cosmetic comprising the steps of preparing an extract in Dongae and adding the extract to the cosmetic ingredient,
The extract manufacturing step in the Dongae etc.
Preparing a powder on Dongae lamp by irradiating microwaves to Dongae lamp and drying it, followed by grinding;
Boiling the powder in distilled water or acetic acid with 0.05 g/mL to 0.2 g/mL in the Dongae etc.;
Recovering the supernatant by centrifugation, and filtering the recovered supernatant in a filter to prepare a pure supernatant; And
Freeze-drying the pure supernatant,
The step of adding the extract to the cosmetic ingredient to the Dongae lamp is a step of adding the extract to the cosmetic ingredient in the freeze-dried Dongae lamp at 50㎍/mL ~ 1000㎍/mL,
The cosmetic ingredient includes water, solubilizer, stabilizer and pigment,
The cosmetic is a method of manufacturing a cosmetic, characterized in that it has a tyrosinase (Tyrosinase) inhibition rate, an elastase (Elastase) inhibition rate, a collagenase (Collagenase) inhibition rate and antioxidant efficacy.

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