KR100646581B1 - The Preparation Method of Nanoparticluated Herb Extracts and The Composition Comprising the Same - Google Patents

Abstract

The present invention relates to a method of preparing a plant herbal extract and to a composition comprising the extract produced thereby. Method for preparing the herbal extract of the present invention is a) mixing the fermented leaves with a honey and fermented, b) adding a concentrated extract to 0.01 ~ 1wt% 7 ~ 10 days before the end of the fermentation reaction of a), c) 10 to 30 parts by weight of honey, 1 to 15 parts by weight of squalene, 0.01 to 5 parts by weight of alcohol, and d) 0.3% hydrogel type glucan to 0.01 to 30 parts by weight of the fermented product. Adding 3 parts by weight. Herbal extracts prepared according to the method of the present invention and compositions comprising the same have high water affinity with water-insoluble substances to improve the effective utilization and applied to the skin or when drinking, excellent delivery power to the tissue and pharmaceutical and medical functionality It is doubled and can be applied to cosmetics and functional foods.

Herb extract, cosmetics, functional food, nanoization, receptor

Description

The Preparation Method of Nanoparticluated Herb Extracts and The Composition Comprising the Same}             

1 shows that the herbal extract is (1) spherical form as a result of the manufacturing process according to the manufacturing process of the herbal extract (2) hydrogel-type glucan is surface-treated and (3) (4) the herbal extract particles by liquid dispersion It is a phenomenon that the sol is activated and separated and moved like the outer surface treatment during the reaction of spherical particles.

Figure 2 shows the process of becoming receptor.

Figure 3 is a distribution of the particle size of the herbal extract of the present invention.

The present invention relates to a method of preparing a plant herbal extract and to a composition comprising the extract produced thereby. More specifically, a method for producing a plant extract having high effective utilization by increasing the water insolubility and water affinity of a potent plant, and a composition containing the herb extract, having excellent delivery power and doubled pharmaceutical and medical functions in cell tissues. The present invention relates to a composition that can be applied to cosmetics, functional foods, and the like.

The desire for human beauty to pursue, protect and cultivate external and internal beauty is endless. Diversification, functionalization, and naturalization of cosmetics are the subjects of great interest. Many studies and efforts have been continued.

However, many cosmetics use a large number of highly toxic chemicals, and it has been found that imidazolidinyl urea, diazolidinyl urea, petrolatum, PVP / VA polymers (PVP / VA Copolymer), Isopropyl Alcohol, Mineral Oil (鑛 油), Propylene Glycol (PG), Sodium Lauryl Sulfate (SLS), Sodium Laures Sulfate (SLES) Sodium laureth sulfate, DEethanolamine (DEA), triethanolamine (TEA), imidazolidinyl Urea, DMDM Hydantoin, and the like. These toxic chemicals are absorbed very easily and quickly through the skin, accumulating in the body's fat and organ tissues, and exhibiting a lot of toxic effects. Of course, it causes contact dermatitis, which interferes with the body's natural moisturizing mechanism, and causes substances such as headache, flushing, dizziness, mental weakness, nausea, vomiting and lethargy, allergies including eye diseases, hair and dry skin, It can cause toxic reactions, joint pain, headaches, chest pain, ear infections, chronic fatigue, insomnia and cough, asthma caused by colds, severe immune system weakness and disturbances, and serious side effects including cancer. Because of this, serious concerns and potential threats to human health are emerging as serious social problems, and it is time for us to desperately need wise wisdom to protect ourselves from this environment and to live a more affluent life. More development is required.

By the way, it is important to increase the utility as a beneficial material even natural materials or materials derived from natural materials. As a useful material, that is, a method for increasing the effective utilization rate, such as atomization of the material, and nanonization. However, even in the case of nanomaterials, it is difficult to secure a large amount of effectiveness when not proceeding to acceptorization.

On the other hand, recently, a herb that is a plant extract ingredient containing the concept of medicine as a natural raw material, for example, "function to protect the skin from the external environment such as pollution", "collagen protein constituting the dermis (Corium) of the skin ), "The ability to prevent skin pigmentation (pigmentation)", etc. can be performed, various researches for applying this herb in real life are being conducted.

As an example of such conventional research results, U.S. Patent No. 5248503 "Herbal dietary supplement" discloses that the "acne of the skin can be suppressed or treated by taking the herb". In another example, US Pat. No. 5869540, "Herbal treatments for improving skin appearance," discloses that "a herb can treat wrinkles and acne on the skin."

Also, well-dried leaves of lemon balm ( Lemonbalm, Melissa officinalis L.) with herbs, Chamomile ( Matricaria recutita L.) leaves are known to have many nutrients and functional ingredients, rich in vitamins and minerals, and have different pharmacological effects from fruits.

The present inventors have completed the present invention by considering that natural materials or materials derived from natural materials undergo physical processes and natural fermentation processes, having safety conditions as an optimal quality product, and that the scope of use can increase the effective expectation and the effect. Was done.

In order to solve the above problems, an object of the present invention is a herb comprising a step of adding a 0.3wt% hydrogel type glucan and adding 0.3wt% hydrogel-type glucan to ferment the herbal leaf with honey and ferment the flavor extract and honey, squalene, alcohol It provides a method for preparing an extract.

Still another object of the present invention is to provide a composition comprising a herb extract and an excipient prepared by the herb extract manufacturing method.

Another object of the present invention is to treat the herb extract and the excipient in the range of 30 minutes to 3 hours with an ultrasonic wave in the range of 30 ~ 60 KHz while liquid phase mixing 1 to 3 hours liquid dispersion mixing at 1,000 rpm with a high speed microdispersion mixer 0.1 It is to provide a composition prepared by incorporating further drop to be ~ 5wt%.

Another object of the present invention to provide a cosmetic or functional food containing 0.01 to 40wt% of the composition as described above.

The present invention is a technique for ultra-microencapsulation and liquid microemulsion of natural herbal extracts, especially insoluble herbal extracts, when nanoparticles of the receptors are applied to the skin to penetrate deeply into the skin to maintain oil and moisture balance and nourish the skin. Favorable for supply

The present invention provides a composition prepared by adding 0.01 to 3 parts by weight of hydrogel type glucan to 100 parts by weight of the herb extract and additionally mixing the alcohol to 0.1 to 5 wt% while liquid-dispersion mixing with a high speed microdispersion mixer.

The present invention comprises the steps of a) mixing the fermented herbs with honey; b) adding the concentrated extract to 0.01 ~ 1wt% 7 ~ 10 days before the end of the fermentation reaction; c) adding 10 to 30 parts by weight of honey, 1 to 15 parts by weight of squalene, and 0.01 to 5 parts by weight of alcohol; And d) provides a method for producing a herbal extract comprising the step of adding to the fermentation 0.3% hydrogel type glucan 0.01 to 3 parts by weight.

The fermenting of the herb leaves by mixing with honey comprises: mixing the herb leaves with honey; Adding bamboo salt to 0.01 to 3wt% of the mixture; And fermenting the mixture at 20 to 30 ° C. under anaerobic conditions.

The herbal extract is a natural extract widely used in cosmetics, such as saponaria extract, willow extract, papaya extract, green tea extract, pine bird sprout extract, rice bran extract, rosemary extract, wild pansy extract, kiwi extract, gentian extract It can be used to collect white iris extract, witch hazel extract, ivy extract, carrot extract, licorice extract, clove extract, white lotus extract, mulberry extract, chamomile extract, lemon balm extract.

The present invention also provides a composition comprising a fermented product before adding the herbal extract and the excipient and / or the concentrated extract prepared by the herbal extract manufacturing method. The excipient is preferably squalene or collagen.

The composition of the present invention is a mixture of the herb extract and the excipient in an ultrasonic range of 30 ~ 60 KHz 30 minutes ~ 3 hours while processing the liquid dispersion and mixing the liquid mass at 1,000 rpm in a high speed microdispersion mixer for 1 to 3 hours It is preferable to be prepared by additionally dropping so as to be 0.1 ~ 5wt% based on the liquid nanoparticles in the liquid fluid phase of the dissociation phase and form a homogeneous phase of 10 ~ 50nm or less.

The present invention provides a cosmetic or health supplement containing the composition as described above 0.01 to 40%.

Currently, almost all cosmetic materials are organic materials as polymer materials, and in recent years, they are progressing as a natural materials. It is an object of the present invention to provide a manufacturing method which may be more advantageous for increasing the effective utilization of natural materials, rather than simply containing cloth burners.

Nano- and carrier technologies are needed to ensure higher utilization rates for natural cosmetics or functional foods.

Since water-soluble substances are dissolved in water and absorbed, nano-sizing is only common sense in insoluble materials. The nano-size at the receptor works in the liquid phase, so even if the nanomaterial does not proceed to acceptorization, it is difficult to secure effective utilization significantly.

In other words, since the receptor is not sufficient as the nano-ization technology, the present invention is a technology for expressing the effectiveness and effective utilization by nano-particle receptor containing a nutrient component that can act on the receptor and fermentation, ripening, extraction, purification of herbs It is a technology to act as a hydrophilic, liquid nanosol type by using the herbal extract obtained as a surface active agent (Surface active agent).

In the present invention, the effective material to be mixed is a micronized component through various processes of manufacturing process in a variety of ways, such as nanonization, delivery system, and sap phase, in order to further increase the characterization properties, fermentation, atomization, and micro Through the process of encapsulation, etc., it increases the effectiveness as a nanosol as a transport fluid.

Normally, the particle size of 10-8 ~ 10-9 m is called nano-size, and the material obtained by the fermentation conditions of the plant extracts in the range of 0.01-1% to the combination of this material can be easily released. Water solubility and water dissociation phase in the paper, and the development of the liquid phase atomization phenomenon to increase the mobility of the size can further increase the effectiveness and utility of the target component.

In the present invention, the fermentation process treatment of the plant extract has the objective of not only as a physical pretreatment process but also as an effective differentiation of the extract and control and removal of harmful microorganisms, eliminating toxic and unnecessary components. Through this, it is possible to obtain nanosol-type plant extracts as liquid ultra-micronized nanoparticles, which are specific to achieve a specific purpose as safety additives and incorporating agents in addition to the basic purpose of eliminating toxicity, and the composition comprising the same ensures safety and doubles effectiveness. Characteristics will be given.

Also important in the present invention are the conditions under which the fragrance extracts (herbs, natural fragrances, etc.) are incorporated in such a way as to achieve optimum effectiveness with or before fermentation, i.

The nanosol liquid product prepared in the present invention was compared with the conventional organic complex chemical-oriented cosmetics as a result of the microbial inhibitory ability was confirmed that the number of microorganisms significantly reduced after 2 days compared to the existing product, which is a liquefied nanosol fine The even dispersion and the effectiveness of the constituents in the particles were judged to be greater.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings and examples, but these drawings and embodiments do not limit the present invention in any way.

Example 1 Preparation of Herb Extracts Through Fermentation and Extraction

Dried leaves of Lemon balm ( Lemonbalm, Melissa officinalis L.) , The leaves of chamomile ( Matricaria recutita L.) were mixed with honey and applied to the range of 0.01% to 3wt% of bamboo salt and fermented for one year at 20 ~ 30 ℃ under anaerobic conditions. 7 ~ 10 days before the end of the reaction, concentrated coconut, tangerine, Chinese quince, grapes, orange concentrated extracts were concentrated by distilling 100cc to 10cc each and then added to 0.01 ~ 1wt% or less before aging. The fermented solution was prepared in an amount of 10 to 30 parts by weight of honey, 1 to 15 parts by weight of squalene, 0.01 to 5 parts by weight of alcohol, and 0.3% of hydrogel type glucan to 0.01 to 3 parts by weight. Thereafter, a composition of 90 to 10 wt% of the fermentation extract before the excipient and / or the flavor concentrate extract was added to the 10 to 90 wt% of the herbal extract was prepared. While treating the composition in the range of 30 to 60 KHz by ultrasonic 30 minutes to 3 hours, 0.1 ~ 5% volume range of the alcohol while mixing liquid dispersion by mixing at 1,000 rpm for 1 to 3 hours with a high speed microdispersion mixer at 1,000 rpm or more. The mixture was further dropped at to prepare a composition in which the extract concentrate had a water dissociation phase in the form of a fine nanosol in the liquid fluid phase and a homogeneous phase having a constitution size of 10 to 50 nm.

Comparative Example 1:

The composition was prepared in the same manner as in Example 1 without adding hydrogel type glucan. No uniform dispersion and homogeneous fine nm size were formed and not formed as a water dissociation phase, resulting in a soft feeling of contact with a grainy feeling of contact.

Example 2: Particle Size of Herb Extract Surface-treated with Hydrogel Type Glucan

The herbal extract surface-treated with the hydrophilic hydrogel type glucan prepared by the method of Example 1 according to the present invention was photographed by a transmission transmission microscope (STEM) in the form of constituent formation and flow utilization in the aqueous phase. 1 and 2 are shown.

1 shows that the herbal extract is (1) spherical form as a result of the manufacturing process according to the manufacturing process of the herbal extract (2) hydrogel-type glucan is surface-treated and (3) (4) the herbal extract particles by liquid dispersion It is a phenomenon that the sol is activated and separated and moved like the outer surface treatment during the reaction of spherical particles.

Figure 2 shows the process of becoming receptor.

There is a problem that it is difficult to calculate the actual effective rate due to a large number of variables and a variable for calculating the effective utilization rate of the effective ingredient of the herbal extract actually used. Therefore, by taking the method of judging by the dissociation phase distribution and size in the water-affinity state of high water content in the body composition, the effective utilization rate of the actual protein can be more effectively measured, and the results are as shown in FIGS. 2 and 3. . The size of the particles of the herb extract of the present invention in the form of nanosol is 50 nm, which is suitable for absorption and dispersion, compared to those of the general general nanoemulsion (300 nm) and the general emulsion (5,000 nm). It was composed of a spherical form, and it was confirmed that the core is easily formed into a spherical form of the nanosol.

Example 3: Dynamic Light Scattering (DSL) Results

The particle size of the composition prepared in Example 1 was measured by DSL (Dynamic Light Scattering) and shown in FIG. 3. According to Figure 3 it can be seen that the average size of the nanosol of the composition of the present invention was made of 14 ~ 50 nm and integrated in a nearly 100% even dispersion range. There were very few particles having a size exceeding 50 nm.

Example 4: Evaluation of Cell Activity-MTT assay

1 × 104 cells / ml in 5% BCS medium and 1 ml each of 24 well plates were seeded and incubated for 24 hours. Then, change to 0.5% BCS medium, treat for a suitable time, remove the medium by aspiration, change to 0.5% serum medium, and treat with 100μl of MTT solution (5mg / ml in PBS buffer, Sigma) for 4 hours in the incubator. It was left for a while. The resulting blue formazan was dissolved in DMSO and absorbance (Hitachi) was measured at 540 nm. As a result, the O.D of the control group was 0.37 and the O.D of Example 1 was 0.5, showing an increase of about 35%. Therefore, the herbal extract of the present invention was confirmed to have an excellent cell proliferation effect.

Example 5: Usability Evaluation

In order to evaluate the degree of skin irritation of the composition of the present Example 1 to 20 women in their 20s to 50 each through the double blind method to evaluate the satisfaction of usability. All of them did not complain of side effects, and they were found to have good feeling and moisturizing, good skin condition and feeling for basic cleaning and washing. 80% or more were satisfied in the freshness, the fragrance persistence, and the usability satisfaction.

The method of preparing the herbal extract according to the present invention and the composition comprising the extract prepared thereby comprises a high herb extract having high effective availability by increasing the water insolubility and water affinity of the effective plant and applying it to the skin or drinking tissue This invention is very useful for the beauty industry and food industry because it has excellent delivery power and doubles pharmaceutical and medical functions.

Claims (10)

0.01 to 3 parts by weight of hydrogel-type glucan is added to 100 parts by weight of the herb extract, and the composition is prepared by additionally mixing the alcohol so that it is 0.1 to 5 parts by weight while mixing liquid dispersion with a high speed microdispersion mixer. a) mixing the herb leaves with honey to ferment; b) adding the concentrated extract to 0.01 ~ 1 parts by weight 7 ~ 10 days before the end of the fermentation reaction of a); c) adding 10 to 30 parts by weight of honey, 1 to 15 parts by weight of squalene, and 0.01 to 5 parts by weight of alcohol; And d) Method of producing a herbal extract comprising the step of adding 0.01 ~ 3 parts by weight of 0.3% hydrogel type glucan to the fermentation mixture. The method of claim 2, wherein the step of fermenting the herbal leaf of a) by mixing with honey a ') mixing the herb leaves with honey; a '') adding bamboo salt to 0.01 to 3 parts by weight of the mixture; And a '' ') fermenting the mixture at 20 ~ 30 ℃ under anaerobic conditions; Method of producing a herb extract, characterized in that it comprises a. According to claim 2 or 3, wherein the herb leaves are lemon balm ( Lemonbalm, Melissa officinalis L.) or Method of producing a herb extract, characterized in that the leaves of chamomile (Chamomile, Matricaria recutita L.) . 10 to 90 parts by weight of the herb extract prepared by the method of claim 2 and excipients or 90 to 10 parts by weight of the fermentation product in step a) of claim 2. 6. The composition of claim 5, wherein the excipient is squalene and / or collagen. According to claim 5, wherein the composition of the herbal extracts and excipients in the 30 ~ 60 KHz range 30 minutes to 3 hours with ultrasonic treatment while the liquid dispersion mixing 1 to 3 hours at 1,000 rpm with a high speed microdispersion mixer The composition characterized in that it is prepared by additionally mixing to fall to 0.1 to 5 parts by weight. The composition according to claim 5, wherein the composition has a water dissociation phase in the form of a fine nanosol in a liquid fluid phase, and forms a homogeneous phase having a constitution size of 10 to 50 nm. A health supplement food comprising 0.01 to 40 parts by weight of the composition of claims 5 to 8.  Cosmetics, characterized in that it comprises 0.01 to 40 parts by weight of the composition of claims 5 to 8.

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