CN111450039A

CN111450039A - Mild and non-irritant moisturizing and anti-aging eye cream and preparation method thereof

Info

Publication number: CN111450039A
Application number: CN202010293279.2A
Authority: CN
Inventors: 唐美荣
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-15
Filing date: 2020-04-15
Publication date: 2020-07-28

Abstract

The invention provides a mild and non-irritant moisturizing and anti-aging eye cream and a preparation method thereof. The raw materials of the eye moisturizing cream are basically natural products, are mild and non-irritant, and through the introduction of the functional components and the construction of a water-in-oil emulsifying system, the functional components can be fully absorbed by eye skin, so that the eye moisturizing cream has good moisturizing and anti-aging effects on the eye skin, and can effectively solve the problem of common wrinkles of eyes.

Description

Mild and non-irritant moisturizing and anti-aging eye cream and preparation method thereof

Technical Field

The invention relates to the technical field of facial skin care products, in particular to mild and non-irritant moisturizing and anti-aging eye cream and a preparation method thereof.

Background

Along with the improvement of living standard and the acceleration of life rhythm of people, people stay up and regularly use computers, mobile phones and the like for a long time, ultraviolet radiation, electromagnetic radiation, chemical stimulation and the like cause the eye skin of people to be injured in different degrees, the eye skin aging is accelerated, and the problems of black eye circles, fine lines, eye bags and the like are caused. The eye cream is a skin care product specially used for eye skin, generally has the effect of moistening and tightening the eye skin, people expect to use the eye cream to solve the common problems of black eye, wrinkles, pouches and the like, but the effect of the existing eye cream product is not satisfactory.

The eye skin is the weakest part of human skin, the total thickness of the eye epidermis and the dermis is about 0.55mm, which is 1/4 of the thickness of the face skin, and the eye skin is more easily damaged. Meanwhile, the sensitive and fragile characteristics of the eye skin also put higher demands on eye cream products, and if efficacy ingredients are pursued, new irritation is brought to the eye skin instead, and the eye cream products are inevitably and irretrievably lost. Most of the existing eye cream products contain a large amount of synthetic chemicals, which often have potential health threats and possibly cause allergy to eye skin. Compared with eye cream products prepared from small parts of all natural ingredients at the other extreme of artificially synthesized chemicals, the eye cream products have the problems that the establishment of an emulsifying system is often lacked, the absorption of eye skin is limited, nutritional ingredients float on the surface, and the eye fat particles are caused by falling over.

Disclosure of Invention

The invention aims to provide a mild and non-irritant moisturizing and anti-aging eye cream and a preparation method thereof, and aims to solve the technical problem that the irritation reduction and efficacy improvement of the existing eye cream product cannot be realized simultaneously.

In order to realize the purpose, the invention provides a preparation method of mild and non-irritant moisturizing and anti-aging eye cream, which comprises the following specific steps in parts by weight:

(1) firstly, adding 1 part of evening primrose oil, 0.02-0.05 part of lecithin and 0.15-0.2 part of lanolin into an emulsifying pot, stirring at 90-95 ℃ until the components are completely dissolved, cooling to 38-45 ℃ to obtain an oil phase, and preserving heat for later use;

(2) then adding 0.005-0.006 part of green tea and 0.01-0.02 part of onion into 0.2-0.3 part of water at 60 ℃, preserving heat, performing ultrasonic oscillation for 5-8 minutes, filtering to obtain filtrate, cooling to room temperature (25 ℃), adding 0.002-0.004 part of trehalose, and stirring until the trehalose is completely dissolved to obtain a water phase;

(3) and then adding 0.002-0.004 parts of yeast glucan into the oil phase, uniformly stirring, slowly adding the water phase into the oil phase while stirring, stirring and emulsifying, cooling to room temperature (25 ℃), finally adding 0.005-0.006 parts of dried camellia cell extract, 0.003-0.004 parts of pawpaw seed lactobacillus leavening and 0.001-0.002 parts of ant extract, and homogenizing to obtain the mild and non-irritant moisturizing and anti-aging eye cream.

Preferably, in the step (3), the preparation method of the camellia stem cell extract is as follows: firstly, cleaning and disinfecting camellia petals in the full bloom stage, inoculating the camellia petals into a first culture medium, culturing to obtain camellia stem cells, then transferring the camellia stem cells into a second culture medium for amplification culture, and performing post-treatment to obtain the camellia stem cell extract.

More preferably, the petals are inoculated by cutting into pieces of 2mm × 2mm, and placing the pieces in a single layer on the surface of the first culture medium.

More preferably, the first culture medium is an aqueous solution and comprises, by weight, 100-110 mg/L of magnesium sulfate, 1000-1110 mg/L of potassium nitrate, 120-130 mg/L of calcium chloride, 2-2.5 mg/L of nicotinic acid, 1-2 mg/L of arginine, 100-110 mg/L-naphthylacetic acid, 13-14 mg/L of sucrose and 3-3.3 mg/L of agar, wherein the culture temperature is 25-29 ℃, the pH is 6-6.5, and the culture time is 20-25 days.

More preferably, the second culture medium is an aqueous solution and comprises, by weight, 150-160 mg/L magnesium sulfate, 800-850 mg/L potassium nitrate, 80-90 mg/L calcium chloride, 3-5 mg/L nicotinic acid, 130-140 mg/L-naphthylacetic acid, 3-5 mg/L sucrose, 50-55 mg/L sucrose and 100-120 mg/L medical stone particles, wherein the culture temperature is 25-29 ℃, the pH value is 5-5.5, and the culture time is 15-20 days.

Further preferably, the post-treatment method comprises the following specific steps: filtering to obtain a solid culture, freezing for 8-10 hours at the temperature of-40 to-50 ℃, naturally thawing, crushing cells by using an ultrasonic crusher, centrifuging to obtain a supernatant, concentrating, and performing vacuum freeze drying to obtain the camellia stem cell extract.

Preferably, in the step (3), the preparation method of the pawpaw seed lactobacillus leavening comprises the following steps: taking ripe papaya seeds, cleaning, airing, crushing into papaya seed powder, transferring 100 parts of papaya seed powder into a fermentation tank, adding water with the temperature of 30-35 ℃ into the fermentation tank, adjusting the mass content of water in the fermentation tank to 35-40%, uniformly scattering 0.03-0.04 part of lactobacillus plantarum powder (ATCC8014, purchased from Shanghai Fuxiang Biotechnology Co., Ltd.), performing anaerobic fermentation at the temperature of 30-35 ℃ for 40-45 hours, and performing post-treatment to obtain the papaya seed lactobacillus fermented product.

Further preferably, the post-treatment method comprises the following specific steps: centrifuging at 10000-12000 rpm for 5-8 minutes, taking supernatant and carrying out pasteurization.

Preferably, in the step (3), the preparation method of the ant extract comprises the following steps: cleaning ants, drying and crushing the ants to obtain ant powder, then carrying out ultrasonic extraction on the ant powder for 30-50 minutes by using an ethanol water solution with the volume concentration of 40-50%, filtering and collecting filtrate, concentrating, and carrying out vacuum freeze drying to obtain the ant extract.

More preferably, the amount of the ethanol aqueous solution is 5 to 8 times of the weight of the ant powder.

Preferably, in the step (3), the water phase is slowly added into the oil phase at a constant speed for 30-40 minutes, and the stirring speed in the feeding process of the water phase is 200-300 r/min.

Preferably, in the step (3), the process conditions of stirring and emulsifying are as follows: stirring for 20-30 minutes at 20-30 ℃ and 2000-3000 r/min.

Preferably, in the step (3), the process conditions of the homogenization treatment are as follows: homogenizing under high pressure for 5-8 cycles under the condition of 50-80 MPa, and then stirring for 5-10 minutes under the conditions of ice bath and 300-400 r/min.

The mild and non-irritant moisturizing and anti-aging eye cream prepared by the preparation method is mild and non-irritant.

The invention has the following beneficial effects:

according to the invention, evening primrose oil, lecithin and lanolin are prepared into an oil phase, green tea, onion and trehalose are prepared into a water phase, yeast glucan is added into the oil phase, the water phase is slowly added into the oil phase, stirring and emulsifying are carried out, cooling is carried out to room temperature (25 ℃), and finally, a tea flower stem cell extract, a pawpaw seed lactobacillus leavening and an ant extract are added, and a water-in-oil eye cream is obtained after homogenization treatment. The raw materials of the eye moisturizing cream are basically natural products, are mild and non-irritant, and through the introduction of the functional components and the construction of a water-in-oil emulsifying system, the functional components can be fully absorbed by eye skin, so that the eye moisturizing cream has good moisturizing and anti-aging effects on the eye skin, and can effectively solve the problem of common wrinkles of eyes.

The invention mainly realizes the construction of an emulsification system through lecithin and lanolin, both have emulsibility as much as possible, but both the lecithin and the lanolin have the problem of stickiness and have poor use feeling. The hydrophobic end of lecithin is in a long chain shape, the hydrophobic end of lanolin is in a ring structure such as a benzene ring, the separation distance of the long-chain structure of lecithin is farther due to the existence of the ring structure of lanolin, the synergistic hydrophobic effect of the hydrophobic ends of the lecithin and the lanolin is better, the hydrophobicity is further enhanced, the emulsification effect is enhanced, the stability of a water-in-oil emulsification system is ensured, the product has a good moisturizing and anti-aging effect, and meanwhile, the greasy feeling of the product is weakened due to the separation between the hydrophobic structures. The trehalose is added, the hydrophilic trehalose contains hydroxyl groups, a part of water molecules can be replaced to form hydrogen bonds with a part of hydrophilic ends of lecithin or lanolin, the trehalose contains rich hydroxyl groups, a part of the hydroxyl groups form hydrogen bonds with the hydrophilic ends of the lecithin and the lanolin, and the rest of the hydroxyl groups form hydrogen bonds with water, so that the content of free water in a system is increased, and the sticky feeling of the product is further weakened.

The evening primrose oil mainly comprises gamma-linoleic acid, vitamins, minerals and the like, has moisturizing effect, and prevents aging; yeast glucan is a bacterial polysaccharide with the effect of repairing cells; the camellia stem cell extract contains rich catechin, vitamin, tannin, amino acid and the like in raw material camellia, has excellent moisturizing and anti-wrinkle effects and good free radical clearing capacity, can promote cell regeneration, recover the activity of cells and strengthen the moisturizing and anti-wrinkle effects; pawpaw seed lactic acid bacteria fermentation product, wherein pawpaw seeds contain pawpaw enzymes, mineral substances and abundant amino acids, lactic acid is generated through lactic acid bacteria fermentation, the pawpaw seed lactic acid bacteria fermentation product has the effects of moisturizing and removing wrinkles on skin on one hand, and has the function of antisepsis on the other hand, so that the shelf life of the obtained eye cream product is prolonged; the ant extract contains a large amount of amino acids, triterpenes (anti-allergy) and the like, can protect the activity of skin fibroblasts, remarkably increase the secretion of collagen and hydroxyproline in the skin, enhance the skin elasticity, have a certain anti-aging effect, improve the activity of superoxide dismutase and reduce the generation of active oxygen, and has a certain anti-oxidation effect. Among the raw materials, the camellia stem cell extract and the pawpaw seed lactobacillus leavening provide plant source amino acid, the ant extract provides animal source amino acid, the amino acid types are enriched, the eye skin is better in nutrition, and the moisturizing and anti-aging effects are improved. The gamma-linoleic acid in the evening primrose oil has small molecular weight and can be absorbed by skin, contains carboxylic acid, can form hydrogen bond with amino acids gathered by camellia stem cell extract, pawpaw seed lactobacillus leavening, ant extract and the like, and is absorbed by the skin along with the gamma-linoleic acid, so that the corresponding eye skin care effect is obtained.

The green tea and the onion are extracted by water ultrasonic oscillation to release tea polyphenol and onion polyphenol, both of which have antioxidation and are used as hydrogen donors to release hydrogen to combine with free radicals in the environment, so that the continuous oxidation process is prevented, the shelf life of the product is prolonged, and the skin aging is delayed. The carbon skeleton structures of tea polyphenol and onion polyphenol are different, the molecular structures of the tea polyphenol and the onion polyphenol are large and small, free radicals with different molecular weights can be captured, and the antioxidant effect is synergistically improved. The tea polyphenol, the onion polyphenol and the pawpaw seed lactobacillus fermentation product have the functions of corrosion prevention and oxidation resistance, and no preservative or antioxidant is required to be added.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

The following is a detailed description of embodiments of the invention, but the invention can be implemented in many different ways, as defined and covered by the claims.

Example 1:

a preparation method of mild and non-irritant moisturizing and anti-aging eye cream comprises the following specific steps in parts by weight:

(1) adding 1 part of evening primrose oil, 0.02 part of lecithin and 0.2 part of lanolin into an emulsifying pot, stirring at 90 ℃ until the components are completely dissolved, cooling to 38 ℃ to obtain an oil phase, and keeping the temperature for later use;

(2) then adding 0.006 part of green tea and 0.01 part of onion into 0.3 part of water with the temperature of 60 ℃, preserving heat and carrying out ultrasonic oscillation for 5 minutes, filtering to obtain filtrate, cooling to room temperature (25 ℃), adding 0.004 part of trehalose, and stirring until the trehalose is completely dissolved to obtain a water phase;

(3) and then adding 0.002 part of yeast glucan into the oil phase, uniformly stirring, slowly adding the water phase into the oil phase while stirring, stirring and emulsifying, cooling to room temperature (25 ℃), finally adding 0.006 part of camellia stem cell extract, 0.003 part of pawpaw seed lactobacillus leavening and 0.002 part of ant extract, and homogenizing to obtain the mild and non-irritant moisturizing and anti-aging eye cream.

Wherein, in the step (3), the preparation method of the camellia stem cell extract comprises the following steps: firstly, cleaning and disinfecting camellia petals in the full bloom stage, inoculating the camellia petals into a first culture medium, culturing to obtain camellia stem cells, then transferring the camellia stem cells into a second culture medium for amplification culture, and performing post-treatment to obtain the camellia stem cell extract.

The petal is inoculated by cutting into 2mm × 2mm, and placing on the surface of the first culture medium in a single layer.

The first culture medium is an aqueous solution and comprises 100 mg/L magnesium sulfate, 1110 mg/L potassium nitrate, 120 mg/L calcium chloride, 2 mg/L nicotinic acid-100 mg/L-naphthylacetic acid 2.5 mg/L sucrose 13 mg/L agar 3.3 mg/L by weight concentration, the culture temperature is 25 ℃, the pH value is 6.5, and the culture time is 20 days.

The second culture medium is an aqueous solution and comprises, by weight, 160 mg/L mg of magnesium sulfate, 800 mg/L mg of potassium nitrate, 90 mg/L mg of calcium chloride, 3 mg/L mg of nicotinic acid, 140 mg/L mg of arginine, 3 mg/L mg of naphthylacetic acid, 55 mg/L mg of cane sugar and 100 mg/L of medical stone particles, wherein the culture temperature is 29 ℃, the pH value is 5, and the culture time is 20 days.

The specific method of post-treatment is as follows: filtering to obtain solid culture, freezing at-40 deg.C for 10 hr, thawing naturally, crushing cells with ultrasonic crusher, centrifuging to obtain supernatant, concentrating, and vacuum freeze drying to obtain the camellia stem cell extract.

In the step (3), the preparation method of the pawpaw seed lactobacillus leavening comprises the following steps: taking ripe papaya seeds, cleaning, airing, crushing into papaya seed powder, transferring 100 parts of papaya seed powder into a fermentation tank, adding 30 ℃ water into the fermentation tank, adjusting the mass content of water in the fermentation tank to be 40%, uniformly scattering 0.03 part of lactobacillus plantarum powder (ATCC8014, purchased from Shanghai Hakka Reineckia Biotech Co., Ltd.), performing anaerobic fermentation at 35 ℃ for 40 hours, and performing post-treatment to obtain the papaya seed lactobacillus fermented product.

The specific method of post-treatment is as follows: centrifuging at 12000 r/min for 5 min, collecting supernatant, and pasteurizing.

In the step (3), the preparation method of the ant extract comprises the following steps: cleaning ants, drying and crushing to obtain ant powder, then carrying out ultrasonic extraction on the ant powder for 50 minutes by using an ethanol water solution with the volume concentration of 40%, filtering, collecting filtrate, concentrating, and carrying out vacuum freeze drying to obtain the ant extract.

The amount of the ethanol water solution is 5 times of the weight of the ant powder.

In the step (3), the water phase is slowly added into the oil phase at a constant speed for 40 minutes, and the stirring speed is 200r/min in the feeding process of the water phase.

In the step (3), the technological conditions of stirring and emulsification are as follows: stirring is carried out for 30 minutes at 30 ℃ and 2000 r/min.

In the step (3), the process conditions of the homogenization treatment are as follows: the mixture was first homogenized under high pressure at 50MPa for 8 cycles and then stirred for 10 minutes in an ice bath at 300 r/min.

Example 2:

(1) adding 1 part of evening primrose oil, 0.05 part of lecithin and 0.15 part of lanolin into an emulsifying pot, stirring at 95 ℃ until the components are completely dissolved, cooling to 45 ℃ to obtain an oil phase, and keeping the temperature for later use;

(2) then adding 0.005 part of green tea and 0.02 part of onion into 0.2 part of water with the temperature of 60 ℃, preserving heat, performing ultrasonic oscillation for 8 minutes, filtering to obtain filtrate, cooling to the room temperature (25 ℃), adding 0.002 part of trehalose, and stirring until the trehalose is completely dissolved to obtain a water phase;

(3) and then adding 0.004 parts of yeast glucan into the oil phase, uniformly stirring, slowly adding the water phase into the oil phase while stirring, stirring and emulsifying, cooling to room temperature (25 ℃), finally adding 0.005 parts of camellia stem cell extract, 0.004 parts of pawpaw seed lactobacillus leavening and 0.001 parts of ant extract, and homogenizing to obtain the mild and non-irritant moisturizing and anti-aging eye cream.

The first culture medium is an aqueous solution and comprises, by weight, 110 mg/L magnesium sulfate, 1000 mg/L potassium nitrate, 130 mg/L calcium chloride, 1 mg/L nicotinic acid-arginine 110 mg/L-naphthylacetic acid 2 mg/L sucrose 14 mg/L agar 3 mg/L, and the culture temperature is 29 ℃, the pH value is 6, and the culture time is 25 days.

The second culture medium is an aqueous solution and comprises 150 mg/L mg of magnesium sulfate, 850 mg/L mg of potassium nitrate, 80 mg/L mg of calcium chloride, 5 mg/L mg of nicotinic acid, 130 mg/L mg of arginine, 5 mg/L mg of naphthylacetic acid, 50 mg/L mg of cane sugar and 120 mg/L mg of medical stone particles by weight concentration, wherein the culture temperature is 25 ℃, the pH value is 5.5, and the culture time is 15 days.

The specific method of post-treatment is as follows: filtering to obtain solid culture, freezing at-50 deg.C for 8 hr, thawing naturally, crushing cells with ultrasonic crusher, centrifuging to obtain supernatant, concentrating, and vacuum freeze drying to obtain the camellia stem cell extract.

In the step (3), the preparation method of the pawpaw seed lactobacillus leavening comprises the following steps: taking ripe papaya seeds, cleaning, airing, crushing into papaya seed powder, transferring 100 parts of papaya seed powder into a fermentation tank, adding 35 ℃ water into the fermentation tank, adjusting the mass content of water in the fermentation tank to 35%, uniformly scattering 0.04 part of lactobacillus plantarum powder (ATCC8014, purchased from Shanghai Reineckia Biotech Co., Ltd.), performing anaerobic fermentation at 30 ℃ for 45 hours, and performing post-treatment to obtain the papaya seed lactobacillus fermented product.

The specific method of post-treatment is as follows: centrifuging at 10000 rpm for 8 min, collecting supernatant, and pasteurizing.

In the step (3), the preparation method of the ant extract comprises the following steps: cleaning ants, drying and crushing to obtain ant powder, then carrying out ultrasonic extraction on the ant powder for 30 minutes by using an ethanol water solution with the volume concentration of 50%, filtering and collecting filtrate, concentrating, and carrying out vacuum freeze drying to obtain the ant extract.

The amount of the ethanol water solution is 8 times of the weight of the ant powder.

In the step (3), the water phase is slowly added into the oil phase at a constant speed for 30 minutes, and the stirring speed is 300r/min in the feeding process of the water phase.

In the step (3), the technological conditions of stirring and emulsification are as follows: stirring at 20 ℃ and 3000r/min for 20 minutes.

In the step (3), the process conditions of the homogenization treatment are as follows: the mixture was first homogenized under 80MPa for 5 cycles and then stirred for 5 minutes in an ice bath at 400 r/min.

Example 3:

(1) adding 1 part of evening primrose oil, 0.03 part of lecithin and 0.18 part of lanolin into an emulsifying pot, stirring at 92 ℃ until the components are completely dissolved, cooling to 42 ℃ to obtain an oil phase, and keeping the temperature for later use;

(2) then adding 0.0055 part of green tea and 0.015 part of onion into 0.25 part of 60 ℃ water, preserving heat, performing ultrasonic oscillation for 5-8 minutes, filtering to obtain a filtrate, cooling to room temperature (25 ℃), adding 0.003 part of trehalose, and stirring until the trehalose is completely dissolved to obtain a water phase;

(3) and then adding 0.003 part of yeast glucan into the oil phase, uniformly stirring, slowly adding the water phase into the oil phase while stirring, stirring and emulsifying, cooling to room temperature (25 ℃), finally adding 0.0055 part of camellia stem cell extract, 0.0035 part of pawpaw seed lactobacillus leavening and 0.0015 part of ant extract, and homogenizing to obtain the mild and non-irritant moisturizing and anti-aging eye cream.

The first culture medium is an aqueous solution and comprises 105 mg/L mg of magnesium sulfate, 1100 mg/L mg of potassium nitrate, 125 mg/L mg of calcium chloride, 1.5 mg/L mg of nicotinic acid, 105 mg/L-arginine, 2.2 mg/L mg of naphthylacetic acid, 13.5 mg/L mg of cane sugar and 3.2 mg/L mg of agar by weight concentration, wherein the culture temperature is 27 ℃, the pH value is 6.2, and the culture time is 22 days.

The second culture medium is an aqueous solution and comprises 155 mg/L mg of magnesium sulfate, 820 mg/L mg of potassium nitrate, 85 mg/L mg of calcium chloride, 4 mg/L mg of nicotinic acid, 135 mg/L of arginine, 4 mg/L mg of naphthylacetic acid, 52 mg/L of cane sugar and 110 mg/L of medical stone particles by weight concentration, wherein the culture temperature is 27 ℃, the pH value is 5.2, and the culture time is 18 days.

The specific method of post-treatment is as follows: filtering to obtain solid culture, freezing at-45 deg.C for 9 hr, thawing naturally, crushing cells with ultrasonic crusher, centrifuging to obtain supernatant, concentrating, and vacuum freeze drying to obtain the camellia stem cell extract.

In the step (3), the preparation method of the pawpaw seed lactobacillus leavening comprises the following steps: taking ripe papaya seeds, cleaning, airing, crushing into papaya seed powder, transferring 100 parts of papaya seed powder into a fermentation tank, adding 32 ℃ water into the fermentation tank, adjusting the water content in the fermentation tank to be 38%, uniformly scattering 0.035 parts of lactobacillus plantarum powder (ATCC8014, purchased from Shanghai Fuxiang Biotechnology Co., Ltd.), performing anaerobic fermentation at 32 ℃ for 43 hours, and performing post-treatment to obtain the papaya seed lactobacillus fermented product.

The specific method of post-treatment is as follows: centrifuging at 11000 r/min for 6 min, taking supernatant, and pasteurizing.

In the step (3), the preparation method of the ant extract comprises the following steps: cleaning ants, drying and crushing to obtain ant powder, then carrying out ultrasonic extraction on the ant powder for 40 minutes by using an ethanol water solution with the volume concentration of 45%, filtering and collecting filtrate, concentrating, and carrying out vacuum freeze drying to obtain the ant extract.

The amount of the ethanol water solution is 6 times of the weight of the ant powder.

In the step (3), the water phase is slowly added into the oil phase at a constant speed for 35 minutes, and the stirring speed is 200r/min in the feeding process of the water phase.

In the step (3), the technological conditions of stirring and emulsification are as follows: stirring is carried out at 25 ℃ and 3000r/min for 25 minutes.

In the step (3), the process conditions of the homogenization treatment are as follows: the mixture was first homogenized under high pressure at 70MPa for 6 cycles and then stirred for 8 minutes in an ice bath at 400 r/min.

Comparative example 1

Lecithin was omitted when the oil phase was prepared.

The rest is the same as example 1. (ii) a

Comparative example 2

Evening primrose oil is omitted during the preparation of the oil phase.

The rest is the same as example 1.

Comparative example 3

Bulbus Allii Cepae can be omitted during preparation of the aqueous phase.

The rest is the same as example 1.

Comparative example 4

The yeast glucan is omitted in step (3).

The rest is the same as example 1.

Comparative example 5

The stem cell extract of the tea flower is omitted in the step (3).

The rest is the same as example 1.

Comparative example 6

In the step (3), the pawpaw seed lactic acid bacteria fermentation product is omitted.

The rest is the same as example 1.

Comparative example 7

In step (3), ant extract is omitted.

The rest is the same as example 1.

Test examples

1. Skin irritation test

40 healthy white guinea pigs are taken, the weight is 250-one blood and 300g, the male and female guinea pigs are used simultaneously, the back hair on two sides is removed before the test, each side is 3 × 3cm, the guinea pigs are randomly divided into 4 groups, wherein 1 group is used as a blank control, the eye cream obtained in the embodiments 1-3 is respectively used for the remaining 3 groups, and the specific use method of the eye cream is that 0.5g of the eye cream obtained in the embodiments 1-3 is applied to a back hair removal area, 1 time every other day and 3 times in total, 14 days after the last contact, the eye cream is applied to the same area of the back, the eye cream is immediately observed after 6 hours of removal, and allergic reactions such as erythema, edema and the like do not occur in 24 hours, 48 hours and 72 hours of observation, which shows that the eye cream is mild and has no irritation.

2. Stability test

The eye creams obtained in examples 1 to 3 and comparative examples 3 and 6 were placed at 25 ℃ in a closed state for 6 months and 24 months, and the change in appearance odor was examined for 2 hours and 12 hours after opening, and the results are shown in table 1.

TABLE 1 stability test results

As is clear from Table 1, the eye creams obtained in examples 1 to 5 showed no change in color and odor during two inspection periods of the sealed and unsealed states, and had good stability. Comparative example 3 omits onion when preparing the aqueous phase, the oxidation resistance becomes poor, resulting in the product stability becoming poor, comparative example 6 omits the papaya seed lactic acid bacteria fermentation in step (3), lacks the preservative effect brought by the papaya seed lactic acid bacteria fermentation, the product stability becomes poor.

3. Examination of the feeling of use and efficacy of eye creams

The results of examining the feel of use and moisturizing and anti-aging effects of the eye creams obtained in examples 1 to 3 and comparative examples 1 to 6 are shown in table 2.

Selecting 90 subjects with healthy skin and no allergic history of skin diseases, wherein the subjects are 25-28 years old and half male and female, randomly dividing the subjects into 9 groups, wherein the number of the male and female in each group is the same, cleaning the whole face by using facial cleanser in a room with the temperature of 25 ℃ and the RH of 80 percent, washing foams by using clean water, and then sucking excessive water by using a paper towel or a dry towel.

The eye creams of examples 1-3 or comparative examples 1-6 are applied to the eye periphery and massaged until absorption, the application amount per eye periphery is 0.05g, the eye cream is applied once in the morning and at night, and the eye cream is continuously used for 15 weeks until the test is finished.

The moisture content of the skin around the eyes was measured separately using a moisture meter, the VISIA meter was used to record the left, right and front lighting of the subject, and the wrinkle score was recorded.

TABLE 2 sense of use and efficacy Studies

As can be seen from Table 2, the eye creams obtained in examples 1-3 have a moist and non-sticky feeling, and have good moisturizing and anti-aging effects.

Comparative example 1 lecithin was omitted when preparing the oil phase, the slimy feeling was significant, and the emulsification effect became poor, affecting the moisturizing anti-aging effect;

comparative example 2 omitting the evening primrose oil when preparing the oil phase, the moisturizing and anti-aging effects are obviously deteriorated, which shows that the evening primrose oil and other functional components have synergistic effect;

comparative example 3 onion was omitted when preparing the aqueous phase, the oxidation resistance was deteriorated and the anti-aging effect was deteriorated;

comparative example 4 yeast glucan was omitted in step (3), comparative example 5 tea stem cell extract was omitted in step (3), comparative example 6 papaya seed lactic acid bacteria fermented product was omitted in step (3), and comparative example 7 ant extract was omitted in step (3), and the moisturizing and anti-aging effects were significantly deteriorated.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A preparation method of mild and non-irritant moisturizing and anti-aging eye cream is characterized by comprising the following specific steps in parts by weight:

(2) then adding 0.005-0.006 part of green tea and 0.01-0.02 part of onion into 0.2-0.3 part of water at 60 ℃, preserving heat, performing ultrasonic oscillation for 5-8 minutes, filtering to obtain filtrate, cooling to room temperature, adding 0.002-0.004 part of trehalose, and stirring until the trehalose is completely dissolved to obtain a water phase;

2. The method according to claim 1, wherein the stem cell extract of camellia sinensis is prepared by the following method in step (3): firstly, cleaning and disinfecting camellia petals in the full bloom stage, inoculating the camellia petals into a first culture medium, culturing to obtain camellia stem cells, then transferring the camellia stem cells into a second culture medium for amplification culture, and performing post-treatment to obtain the camellia stem cell extract.

3. The method according to claim 2, wherein the first medium is an aqueous solution and comprises, in terms of weight concentration, 100 to 110 mg/L of magnesium sulfate, 1000 to 1110 mg/L of potassium nitrate, 120 to 130 mg/L of calcium chloride, 100 to 2 mg/L of nicotinic acid, 100 to 110 mg/L of arginine, 2 to 2.5 mg/L of naphthylacetic acid, 13 to 14 mg/L of sucrose, and 3 to 3.3 mg/L of agar, and the culture temperature is 25 to 29 ℃, the pH is 6 to 6.5, and the culture time is 20 to 25 days.

4. The preparation method of claim 2, wherein the second culture medium is an aqueous solution comprising, by weight, 150 to 160 mg/L mg of magnesium sulfate, 800 to 850 mg/L mg of potassium nitrate, 80 to 90 mg/L mg of calcium chloride, 3 to 5 mg/L mg/3 to 5 mg/L mg of nicotinic acid, 130 to 140 mg/L mg of arginine, 50 to 55 mg/L mg of sucrose, and 100 to 120 mg/L of Maifanitum granules, and the culture temperature is 25 to 29 ℃, the pH is 5 to 5.5, and the culture time is 15 to 20 days.

5. The preparation method according to claim 2, characterized in that the post-treatment is carried out by the following specific method: filtering to obtain a solid culture, freezing for 8-10 hours at the temperature of-40 to-50 ℃, naturally thawing, crushing cells by using an ultrasonic crusher, centrifuging to obtain a supernatant, concentrating, and performing vacuum freeze drying to obtain the camellia stem cell extract.

6. The method according to claim 1, wherein the papaya seed lactic acid bacteria fermented product in the step (3) is prepared as follows: taking ripe papaya seeds, cleaning, airing, crushing into papaya seed powder, transferring 100 parts of papaya seed powder into a fermentation tank, adding water with the temperature of 30-35 ℃ into the fermentation tank, adjusting the mass content of water in the fermentation tank to 35-40%, uniformly scattering 0.03-0.04 part of lactobacillus plantarum powder, carrying out anaerobic fermentation at the temperature of 30-35 ℃ for 40-45 hours, and carrying out post-treatment to obtain the papaya seed lactobacillus fermented product.

7. The method as set forth in claim 1, wherein the ant extract is prepared by the following method in step (3): cleaning ants, drying and crushing the ants to obtain ant powder, then carrying out ultrasonic extraction on the ant powder for 30-50 minutes by using an ethanol water solution with the volume concentration of 40-50%, filtering and collecting filtrate, concentrating, and carrying out vacuum freeze drying to obtain the ant extract.

8. The preparation method according to claim 1, wherein in the step (3), the process conditions of stirring and emulsifying are as follows: stirring for 20-30 minutes at 20-30 ℃ and 2000-3000 r/min.

9. The method according to claim 1, wherein in the step (3), the process conditions of the homogenization treatment are as follows: homogenizing under high pressure for 5-8 cycles under the condition of 50-80 MPa, and then stirring for 5-10 minutes under the conditions of ice bath and 300-400 r/min.

10. The mild and non-irritant moisturizing and anti-aging eye cream prepared by the preparation method of any one of claims 1-9.