CN110872568A - Composition for preventing or improving aging comprising Bacillus pumilus strain or its culture solution - Google Patents
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Abstract
The present disclosure relates to a cosmetic composition comprising a Bacillus pumilus (Bacillus pumilus) strain or a culture thereof. The cosmetic composition according to an aspect of the present disclosure has an excellent effect of preventing or improving skin aging, or preventing or improving skin wrinkles, or regenerating skin by including a bacillus pumilus strain or a culture solution thereof.
Description
Technical Field
The present disclosure relates to a composition for preventing or improving aging comprising a Bacillus pumilus (Bacillus pumilus) strain or a culture solution thereof.
Background
In the field of functional cosmetics, there is a great interest in products for delaying skin aging to help improve skin wrinkles, products for protecting skin from ultraviolet rays and external environments, and products for helping skin whitening. In fact, with the introduction into aging society, there is an ongoing effort to develop wrinkle-improving cosmetics for delaying skin aging. Generally, the main causes of the appearance of wrinkles and reduced skin elasticity, phenomena of skin sloughing and drying, etc., on aged skin are changes in the matrix proteins present in the dermis. Since the role of the dermis in the skin is to support the strength and morphology of the skin, it plays a very critical role in wrinkle formation and skin sloughing when this region undergoes morphological changes during the aging process. Matrix Metalloproteinases (MMPs) are representative enzymes that degrade these matrix proteins, and even a single uv irradiation increases the activity of MMPs in the skin and degrades collagen and other matrix proteins in the skin, as a major factor in promoting aging.
Among skin aging, photo aging is considered to be the largest factor causing skin aging, and many products for preventing photo aging have been on the market, but photo aging prevention techniques using natural materials are limited in their use and have a limitation in that many problems occur in efficacy verification.
In one aspect, Probiotics (Probiotics) is a generic term for microorganisms beneficial to the human body, referring to microorganisms beneficial to our body (benefit). Most of the probiotics known so far are called lactic acid bacteria. Although probiotics have been reported to produce effective efficacy in humans through several beneficial effects, studies on the interrelationship between microorganisms in the genus bacillus and the skin have been incomplete in practice.
Accordingly, the inventors of the present disclosure have confirmed that a microorganism of the genus bacillus or a culture thereof can be effectively used for improving skin conditions by alleviating skin aging, and completed the present disclosure.
Disclosure of Invention
One aspect of the present disclosure provides a strain of Bacillus pumilus (Bacillus pumilus).
Another aspect of the present disclosure provides a cosmetic composition for preventing or improving aging, comprising a bacillus pumilus strain or a culture solution thereof as an effective ingredient.
In another aspect of the present disclosure, there is also provided a use of a strain of bacillus pumilus or a culture thereof for preparing a cosmetic composition.
Another aspect of the present disclosure also provides a use of a strain comprising bacillus pumilus or a culture thereof for preparing a cosmetic composition in a cosmetic composition.
In another aspect of the disclosure, a method of preparing a strain of bacillus pumilus or a culture solution thereof is also provided.
One aspect of the present disclosure provides a Bacillus pumilus strain (accession number: KCCM 12274P).
Another aspect of the present disclosure provides a cosmetic composition for preventing or improving skin aging, comprising a bacillus pumilus strain or a culture solution thereof as an effective ingredient.
In another aspect of the present disclosure, there is also provided a use of a strain of bacillus pumilus or a culture thereof for preparing a cosmetic composition.
In the present disclosure, the term "cosmetic composition" refers to an article for human use that increases attractiveness and changes appearance to be bright or maintains or enhances skin and hair health by performing cleaning and beautifying on the human body.
The cosmetic composition can be used for preventing or improving skin wrinkles. The cosmetic composition can be used for increasing skin elasticity. The cosmetic composition can be used for preventing or improving skin aging or pigmentation. The cosmetic composition can be used for skin regeneration. The cosmetic composition may be used to promote the formation or regeneration of skin tissue. The Bacillus pumilus strain or its culture solution has an excellent effect of preventing or improving skin aging or wrinkles and has an excellent effect of promoting skin regeneration, compared to other Bacillus strains such as Bacillus subtilis or Malva verticillata extract known to have an anti-aging effect, and thus the composition comprising the Bacillus pumilus strain or its culture solution can be effectively used in cosmetic compositions.
In the present disclosure, the term "Bacillus pumilus" refers to a gram-positive, aerobic, spore-forming bacterium commonly found in soil. The Bacillus pumilus can be isolated from food, soil, ocean, etc.
The strain may be Bacillus pumilus CX-UV strain (accession No.: KCCM 12274P).
In the present disclosure, the term "skin aging" refers to a general term for tangible and intangible changes occurring on the skin with the increase of age, for example, thinning of the thickness of the epidermis, the number of dermal cells or blood vessels, the ability to restore DNA damage, the cell replacement cycle, wound healing, skin barrier function, moisture maintenance of the epidermis, sweat secretion, sebum secretion, vitamin D production, protection from physical damage, chemical removal ability, immune response, sensory function, and reduction of thermoregulation. The Bacillus pumilus strain or its culture solution can be used for improving skin aging caused by exogenous factors or endogenous factors. The exogenous factors refer to various exogenous factors such as ultraviolet rays (light), and the endogenous factors refer to factors mainly caused by the passage of time, also referred to as time factors. Specifically, the skin aging includes not only a premature aging symptom caused by external stimulation such as ultraviolet rays, public nuisance, cigarette smoke, and chemicals, but also a natural aging phenomenon in which the proliferation of skin cells is reduced due to aging, which is a concept including wrinkles, elastic reduction, skin sloughing, and drying. In addition, wrinkles include wrinkles caused by the change of the intrinsic factor and the extrinsic factor to change the components constituting the skin tissue.
The aging may be photoaging. The term "photoaging" refers to a phenomenon caused by external environmental factors, with ultraviolet rays being the most typical factor. Ultraviolet light causes activation of proteases, chain scission of matrix proteins, and damage of biological components such as abnormal cross-linking, and repetition of this mechanism leads to apparently apparent skin aging.
In the present disclosure, the term "wrinkle" refers to a state in which the skin loses elasticity and is relaxed, and for example, may be a state in which the skin is folded. Pigmentation means a state in which the amount of pigment in the body is abnormal or where pigment appears, and may be, for example, spots, freckles, and the like. The "skin wrinkle prevention or improvement" may refer to all applications of inhibiting the expression of these wrinkle-associated factors to prevent or improve wrinkles, or increasing the total amount of collagen.
In the present disclosure, the term "ameliorating" may refer to a parameter associated with alleviating or treating a condition, e.g., all actions that may at least reduce the extent of symptoms.
In the present disclosure, the term "preventing" may refer to any act of inhibiting or retarding skin photoaging, pigmentation, or wrinkles by applying the composition.
In the present disclosure, the term "culture solution" refers to a culture solution itself obtained by culturing a strain, a concentrate or a freeze-dried product thereof, or a culture supernatant obtained by removing a strain from a culture solution, a concentrate or a freeze-dried product thereof. The culture broth may include useful active ingredients, such as proteins produced during the cultivation of the bacillus pumilus strain.
The culture may be, but is not limited to, a suspension culture at about 25 ℃ to about 30 ℃ for about 45 hours to about 50 hours. The culture temperature and culture time may be appropriately selected within the range in which the strain exhibits an effect of improving or preventing aging.
The composition can inhibit the expression of matrix metalloproteinase-1 (MMP-1), specifically, the expression of MMP-1 gene or the activity of MMP-1 protein, thereby showing the effect of inhibiting skin aging.
The composition can increase collagen synthesis. The composition can increase the expression of collagen, such as type 1 collagen gene. Specifically, the expression of the type 1 collagen gene can be increased to restore the production of collagen, thereby exhibiting an effect of inhibiting skin aging or an effect of improving wrinkles.
The composition may include an effective amount of a culture fluid. The effective amount may be appropriately selected depending on the individual. The effective amount may be determined based on factors including the following, as well as other factors well known in the physiological to medical arts: the severity of the disease condition, the age, weight, health, sex of the individual, the sensitivity of the individual to the extract, the time of administration, the route of administration and rate of excretion, the period of administration, other compositions used in conjunction or concomitantly with the composition.
Specifically, the culture fluid may include 0.001 to 80 weight percent, for example, 0.01 to 60 weight percent, 0.01 to 40 weight percent, 0.01 to 30 weight percent, 0.01 to 20 weight percent, 0.01 to 10 weight percent, 0.01 to 5 weight percent, 0.05 to 60 weight percent, 0.05 to 40 weight percent, 0.05 to 30 weight percent, 0.05 to 20 weight percent, 0.05 to 10 weight percent, 0.05 to 5 weight percent, 0.1 to 60 weight percent, 0.1 to 40 weight percent, 0.1 to 30 weight percent, 0.1 to 20 weight percent, based on the total weight of the cosmetic composition, 0.1 to 10 weight percent, or 0.1 to 5 weight percent.
The ingredients included in the cosmetic composition may include, in addition to the composition, ingredients conventionally used in cosmetic compositions as effective ingredients, for example, conventional adjuvants and carriers for stabilizers, solvents, vitamins, pigments, perfumes, and the like.
In the case of the composition, the strain or a culture solution thereof may be prepared into a formulation including: lotions (lotions), emollients, lotions, astringents (astringents), lotions, milk lotions (milk lotions), moisturizing lotions, nutritional liquids, massage creams, nutritional creams, moisturizing creams, hand creams, foundations, essences, nourishing essences, films, soaps, cleansing foams, cleansers, cleansing creams, body lotions, body cleansing liquids, suspensions, gels, powders, pastes (pastes), masks or sheet masks, or spray compositions. Compositions of such dosage forms may be prepared according to methods conventional in the art. The cosmetic composition may further comprise preservatives, stabilizers, surfactants, solvents, moisturizers, emollients, ultraviolet absorbers, preservatives, bactericides, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cold sensates or antiperspirants. The mixing amount of the additional ingredients such as the above-mentioned moisturizer and the like can be easily selected by those skilled in the art within a range not to impair the object and effect of the present disclosure, and can be 0.001 to 5 weight percent, specifically, 0.01 to 3 weight percent based on the total weight of the composition.
In addition, the composition can be formulated into a skin external preparation.
In the present disclosure, the external preparation for skin may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a medicated bandage, an emulsion, or a combination thereof. The skin external preparation may be appropriately mixed with ingredients of skin external preparations generally used in cosmetics, pharmaceuticals, and the like, for example, aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, perfumes, colorants, various skin nutrients, and the like, as necessary. The skin external preparation can be mixed with appropriate amount of disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, metal chelating agent such as gluconic acid, caffeine, tannin, verapamil, Glycyrrhrizae radix extract, glabridin, hot water extract of Cirsium japonicum fruit, various crude drugs, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts, and saccharides such as vitamin C, magnesium ascorbyl phosphate, ascorbyl glucoside, arbutin, kojic acid, glucose, fructose, and trehalose.
Another aspect of the present disclosure provides a method of improving skin in an individual comprising administering the composition to the individual. The composition is as described above.
The method may be, in particular, a method of preventing or ameliorating skin aging or preventing or ameliorating skin wrinkles in an individual.
The terms "applied", "administered", "introduced" and "transplanted" are used interchangeably and may refer to a method of achieving at least partial localization of a composition according to one embodiment to a desired site or placement of a composition according to one embodiment into an individual according to a route. The culture fluid or at least a portion of a culture fluid component of a composition according to one embodiment may be administered by any suitable route for delivery to a desired location in a living body.
The subject may be a mammal, for example: human, bovine, equine, porcine, canine, ovine, caprine or feline. The subject may be a subject in need of improvement of skin conditions such as improvement of skin aging or improvement of skin wrinkles.
Another aspect of the present disclosure provides a method of preparing a broth of the bacillus pumilus strain, the method comprising: culturing the Bacillus pumilus strain; cells were separated and removed from the cell culture solution obtained after the culture.
The Bacillus pumilus strain is the same as described above.
According to a specific embodiment, the method may comprise: culturing the Bacillus pumilus strain; centrifuging a cell culture solution obtained after the culture to remove cells and recovering a supernatant; and filtering the recovered supernatant to remove residual cells.
In the above method, the accession number of the Bacillus pumilus strain may be KCCM 12274P.
The culture may be, but is not limited to, a culture at about 25 ℃ to about 30 ℃ for about 45 hours to about 50 hours. The culture may be a suspension culture.
The culture solution of bacillus pumilus strain prepared by the method may increase the expression of MMP-1 to enhance skin barrier or prevent aging, and may show the effect of preventing skin aging or preventing or improving skin wrinkles by increasing the expression of type 1 collagen or restoring the decreased expression of type 1 collagen. The Bacillus pumilus strain or its culture has enhanced skin barrier, skin moisturizing, skin aging prevention, skin wrinkle improvement or skin regeneration effects compared to other Bacillus strains. Therefore, the Bacillus pumilus strain or the culture solution thereof prepared by the method can be used for various applications such as cosmetics and skin external preparations.
Drawings
FIG. 1 is a graph evaluating the efficacy of Bacillus pumilus strains or their culture solutions in inhibiting MMP-1 expression increased by UV treatment. Wherein, CON: UVB untreated control group; UV: a UVB treatment group; CM: a culture medium treatment group to which a malva extract is added after UVB treatment; NASA 1: a culture solution treatment group of a bacillus pumilus strain 15 seconds after UVB treatment; NASA 4: a culture solution treatment group of a bacillus pumilus strain 30 seconds after UVB treatment; RL: UVB-treated strain treatment group of bacillus.
Fig. 2 is a graph evaluating the efficacy of bacillus pumilus strains or its culture solutions in restoring type 1 collagen expression decreased by uv treatment. Wherein, CON: a UVA untreated control group; UVA: a UVA treatment group; CM: a medium treatment group to which a malva extract is added after UVA treatment; FM: a UVA-treated culture solution of a Bacillus pumilus strain and a dead bacteria extract treatment group; FMP: a UVA-treated culture solution treatment group of Bacillus pumilus strains; PNA: strain treatment group of bacillus after UVA treatment.
Detailed Description
Hereinafter, the present disclosure will be described in more detail by examples. However, these embodiments are merely intended to describe one or more specific embodiments by way of example, and the scope of the present disclosure is not limited to these embodiments.
Example 1 isolation and species identification of Bacillus pumilus strains
Soil and dry reed known to distribute a large amount of bacillus subtilis were collected, 100g of a sample of mixed soil and reed was suspended in sterile distilled water at 100rpm for 1 hour, and 10ml of the supernatant was diluted to 1/10, 1/100, 1/1000 and inoculated in R2A, TSA, NA medium. After culturing in an incubator at 25 ℃ for 48 hours, only white and beige circular colonies were selected from the formed colonies and subjected to pure isolation culture. The isolated colonies were again subjected to pure isolation culture and the colony-forming plates were submitted to analysis by Macrogen to identify 30 selected species.
As a result, it was found that two species of bacteria have 98% or more homology with Bacillus pumilus, and the balance are microorganisms of different species or different systems. From the two identified Bacillus pumilus species, the species with the stronger UV resistance were selected. The isolated strain was named Bacillus pumilus CX-UV and was deposited in Korea center for preservation of microorganisms at 2018, 6 months and 14 days under the accession number KCCM 12274P.
Example 2 preparation of Bacillus pumilus Strain and culture solution of Bacillus pumilus Strain
For culturing Bacillus pumilus CX-UV strain, a medium containing R2 as a main component was used. 5g of R2 medium was suspended and dissolved in 1 liter of water, and sterilized at 121 ℃ for 15 minutes to prepare a medium. At this time, two kinds of culture media were prepared: one is solid culture medium containing agar; the other is agar-free liquid medium. First, a strain of Bacillus pumilus CX-UV was spread on a solid medium, cultured at 28 ℃ for 3 days, and the cultured strain of Bacillus pumilus CX-UV was irradiated under UV light for 30 seconds. One of the UV-treated colonies was inoculated into 1 liter of liquid medium. The inoculated liquid medium was subjected to suspension culture (100rpm) at 28 ℃ for 48 hours, and when the culture was completed, only the supernatant from which the cells had been removed was taken out after centrifugation (7000rpm, 30 minutes) by a centrifugal separator, and filter-sterilized by a 0.2 μm filter.
Test example 1 evaluation of efficacy of Medium for Bacillus pumilus Strain on MMP-1
The effect of the culture solution of the Bacillus pumilus strain prepared in example 1 on MMP-1 activity and expression was confirmed.
To evaluate the inhibitory effect of MMP-1 at the tissue level, experiments were performed using EpiDermFTTM (MatTek, USA) in three-dimensional artificially cultured skin tissue.
EpiDermFT comprising first the epidermis and dermis togetherTMAfter stabilization in the medium for 24 hours, the medium was removed, UVB of 60mJ/cm2 was irradiated to the remaining experimental groups except the UVB-unirradiated group, and then fermentation lysates of Bacillus pumilus strains or comparative experimental strains were treated. Subsequently, the malva verticillata extract known to have the effect of improving skin aging or skin wrinkles was replaced with a culture medium added at a concentration to further culture for 12 hours. Subsequently, the medium in which the artificial skin tissue was cultured was collected, and the floating matter was removed by centrifugation for measuring the amount of protein contained in the medium. The MMP-1 protein content of the medium was measured using the MMP-1ELISA kit (EMD Millipore, USA) according to the method described therein. The results of measuring the change in MMP-1 expression are shown in FIG. 1, and the experimental groups are as follows.
CON: UVB untreated control group; UV: a UVB treatment group; CM: a culture medium treatment group to which a malva extract is added after UVB treatment; NASA 1: a culture solution treatment group of a bacillus pumilus strain 15 seconds after UVB treatment; NASA 4: a culture solution treatment group of a bacillus pumilus strain 30 seconds after UVB treatment; RL: UVB-treated bacillus subtilis) strain treatment group.
As shown in FIG. 1, MMP-1 expression was increased in the UVB irradiation group (UV), while MMP-1 expression was slightly decreased in the experimental group (CM) in which culture was resumed in the medium to which the extract of malva was added after UVB treatment, as compared with the UV-treated group. The group (NASA1, NASA4) treated with the culture broth of Bacillus pumilus strains after UVB treatment showed intentionally reduced MMP-1 expression levels compared to these, and also showed more reduced MMP-1 expression levels compared to the other groups (RL) treated with the strains of Bacillus.
Thus, it was confirmed that the treatment by Bacillus pumilus strain reduced the expression of MMP-1 whose expression was increased by UV irradiation. As can be seen from the above results, the Bacillus pumilus strain inhibited the activity of MMP-1 to exhibit the effect of inhibiting skin aging.
Test example 2 evaluation of the efficacy of the culture Medium of Bacillus pumilus Strain on type 1 collagen
The effect of the culture solution of the Bacillus pumilus strain prepared in example 1 on the expression of type 1 collagen was confirmed.
To evaluate the effect of type 1 procollagen increase at tissue level, experiments were performed using the same tissues and under the same conditions as in example 1, except that UVA was used instead of UVB at the time of treatment. Finally, the content of Procollagen type 1 Procollagen in the medium was measured using Procollagen type I C peptide (PIP) EIA kit (Takara-bio inc., japan) according to the method described therein. The measurement results are shown in fig. 2, and the experimental groups are as follows.
CON: a UVA untreated control group; UVA: a UVA treatment group; CM: a medium treatment group to which a malva extract is added after UVA treatment; FM: a UVA-treated culture solution of a Bacillus pumilus strain and a dead bacteria extract treatment group; FMP: a UVA-treated culture solution treatment group of Bacillus pumilus strains; PNA: strain treatment group of bacillus after UVA treatment.
As shown in fig. 2, the content of type 1 procollagen in the medium of the UVA-irradiated group was lower than that of the non-irradiated group (CON), and thus it was confirmed that type 1 procollagen was inhibited by UV treatment. Furthermore, since the treatment group treated the culture broth of Bacillus pumilus strain significantly increased its content, it was confirmed that the ultraviolet-inhibited collagen expression was restored by Bacillus pumilus strain. The effect was significantly higher than that of the group of Bacillus subtilis (PNA) which was treated with another strain of Bacillus.
Therefore, since it was confirmed that the culture solution of Bacillus pumilus strain inhibits the expression of MMP-1 gene and the activity of MMP-1 in cells after UV treatment and restores the expression of type I collagen reduced by ultraviolet rays, it can be used for alleviating, improving and preventing skin aging including photoaging.
The cosmetic composition according to an aspect of the present disclosure has an excellent effect of preventing or improving skin aging, or preventing or improving skin wrinkles, or regenerating skin by including a bacillus pumilus strain or a culture solution thereof.
While certain exemplary embodiments and implementations have been described herein, other embodiments and modifications will be apparent from this description. The inventive concept is therefore not limited to the embodiments but is to be accorded the widest scope consistent with the claims appended hereto, and various modifications and equivalent arrangements will be apparent to those skilled in the art.
Claims (12)
1. A Bacillus pumilus strain (preservation number: KCCM 12274P).
2. A cosmetic composition comprises Bacillus pumilus strain or its culture solution as effective component.
3. The cosmetic composition of claim 2, wherein,
the preservation number of the bacillus pumilus strain is KCCM 12274P.
4. The cosmetic composition of claim 2, wherein,
the composition is used for preventing or improving skin aging or for preventing or improving skin wrinkles.
5. The cosmetic composition of claim 2, wherein,
the skin ages to photoaging.
6. The cosmetic composition of claim 2, wherein,
the bacillus pumilus strain or the culture solution thereof includes 0.01 weight percent to 10 weight percent based on the total weight of the cosmetic composition.
7. The cosmetic composition of claim 2, wherein,
the composition inhibits the expression of matrix metalloproteinase-1 (MMP-1).
8. The cosmetic composition of claim 2, wherein,
the composition increases expression of the type 1 collagen gene.
9. A method of preparing a broth of a strain of bacillus pumilus, the method comprising:
culturing a bacillus pumilus strain; and
cells were separated and removed from the cell culture solution obtained after the culture.
10. The method of claim 9, wherein,
the culturing is performed under conditions of 25 ℃ to 30 ℃ and 45 hours to 50 hours.
11. Use of the Bacillus pumilus strain of claim 1 for the preparation of a cosmetic for preventing or improving skin aging or skin wrinkles.
12. Use of the cosmetic composition according to claim 2 for the preparation of a cosmetic for preventing or improving skin aging or skin wrinkles.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2018-0103039 | 2018-08-30 | ||
| KR1020180103039A KR102065905B1 (en) | 2018-08-30 | 2018-08-30 | Composition for anti-aging comprising Bacillus pumilus strain or culture solution of the strain |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102597744B1 (en) * | 2021-09-17 | 2023-11-03 | 코스맥스 주식회사 | Bacillus infantis HB2 strain and its use for improving skin conditions |
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| CN103205383A (en) * | 2013-04-15 | 2013-07-17 | 淮海工学院 | Bacillus pumilus E14, culturing method thereof and application |
| KR20150003050A (en) * | 2013-06-28 | 2015-01-08 | 주식회사 에스테르 | A composition for anti-inflammation |
| CN104997721A (en) * | 2015-08-06 | 2015-10-28 | 常州思宇环保材料科技有限公司 | Preparation method for skin aging resistant original fluid |
| US20170014334A1 (en) * | 2014-03-31 | 2017-01-19 | Lubrizol Advanced Materials, Inc | Ferment extract of a bacterial strain for the increase of adiponectin levels |
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| KR101426191B1 (en) * | 2013-01-07 | 2014-07-31 | (주)미애부생명과학 | Cosmetic Composition Having Culture Fluid of Lactobacillus sp. and Stem cells |
| KR101719723B1 (en) * | 2014-10-28 | 2017-03-24 | (주)에이씨티 | Cosmetic composition comprising the extract of Ensete lasiocarpum as active ingredient |
| KR101768026B1 (en) * | 2015-07-08 | 2017-08-14 | 주식회사 코리아나화장품 | Cosmetic Composition for Improving Skin Wrinkle Comprising the Fermented Extract of Morinda citrifolia as Active Ingredient |
| KR101902988B1 (en) * | 2016-09-30 | 2018-10-01 | (주)에이씨티 | Cosmetic composition comprising the extract of cacao nibs irradiated with far-infrared ray and steamed |
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| CN103205383A (en) * | 2013-04-15 | 2013-07-17 | 淮海工学院 | Bacillus pumilus E14, culturing method thereof and application |
| KR20150003050A (en) * | 2013-06-28 | 2015-01-08 | 주식회사 에스테르 | A composition for anti-inflammation |
| US20170014334A1 (en) * | 2014-03-31 | 2017-01-19 | Lubrizol Advanced Materials, Inc | Ferment extract of a bacterial strain for the increase of adiponectin levels |
| CN104997721A (en) * | 2015-08-06 | 2015-10-28 | 常州思宇环保材料科技有限公司 | Preparation method for skin aging resistant original fluid |
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| STEFAN HANDTKE等: ""Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress"", 《PLOS ONE》 * |
| 程杰杰等: ""短小芽孢杆菌表面活性素基因克隆及其抑菌活性研究"", 《中国植保导刊》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111544371A (en) * | 2020-06-12 | 2020-08-18 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
| CN111544371B (en) * | 2020-06-12 | 2020-11-20 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
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| KR102065905B1 (en) | 2020-01-14 |
| CN110872568B (en) | 2023-05-26 |
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