KR101132392B1 - Fermented Placenta Compositin and Use Thereof - Google Patents

Abstract

The present invention discloses fermented placental compositions and uses thereof. Specifically, the present invention discloses a fermented placenta composition obtained by pulverizing a mammalian placenta, adding a proper carbon source such as lactic acid bacteria and an energy source to the mixture, fermenting the mixture with lactic acid bacteria, yeast and / or Bacillus sp. .

Fermentation, placenta, bacillus, lactic acid bacteria, yeast

Description

Fermented Placenta Composite and Use Thereof < RTI ID = 0.0 >

The present invention relates to fermented placental compositions and uses thereof.

The placenta is an organ that performs the role of substance exchange while maintaining close contact between the exhaust duct and maternal tissues. The fetus receives nutrients necessary for growth through the placenta.

The placenta contains various nutrients such as proteins and vitamins, so it is regarded as a valuable medicinal material in the main herbarium and Dongbokgol, and there is a record that it has already been used for medicinal purposes in the West even in the West.

Recently, as the regulations on the use of human body have been strengthened, the use of livestock-derived placenta such as cattle, sheep, pigs, and horses is gradually increasing.

Representative nutritional components contained in the placenta include amino acids such as leucine, lysine, valine, threonine, isoleucine, glycine, alanine and arginine, proteins such as albumin and globulin, lipids such as cholesterol, phosphatidic acid, lauric acid, It is also possible to use vitamin C such as vitamin C, potassium, sodium, phosphorus, magnesium, etc., such as carbohydrates such as fatty acid, glucose, and galactose, mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, vitamins B1, B2, B6, B12, C, D, E, Zinc, and iron; DNA, RNA, and metabolites thereof; and nucleic acids, alkaline phosphatase, and hyaluronidase.

These placentas are known to contain similar nutritional values and ingredients, although there may be some differences in nutrient concentrations depending on the type of livestock. In other words, most of the placenta in addition to the three major nutrients, enzymes, vitamins, minerals and reports of the placenta obtained from the extract of the extract to stimulate collagen synthesis, improve immunity. In relation to the skin, it exhibits a function to prevent drying, to make it lively and vigorous.

  In recent years, allergies caused by immunological abnormalities have occurred at a rapid rate in the world. Placenta extract has a great effect on autoimmunity control, and it has been reported that it plays a role of enhancing immunity and lowering immunity (J. Mol. Endocrinol., 2007, 39, 189-198).

In addition, it can be used for the treatment of metabolic function, regulating hormone secretion, fatigue recovery, anti-inflammatory and antiallergic action, blood circulation promoting action, cell division promoting action, liver function strengthening, antistress action, Melanin synthesis inhibitory action, autonomic nervous control action, and hematopoietic action have been reported.

 These various physiological activities are understood as enhancing the natural healing power of the body by promoting metabolism or function of cells or tissues while harmonizing them in the human body.

On the other hand, general extraction process of fetus or livestock-derived placenta is performed by removing water with a solvent such as butanol or nucleic acid and then performing water-soluble extraction using water containing salts such as water or ammonium sulfate, Acid, alkali, proteolytic enzyme or the like, and the like. Conventional processes for obtaining a placenta extract in general have been carried out by adopting some of the above methods. Enzymes, alcohols and the like may be used again in order to obtain a finer low molecular weight material from the placenta extract obtained by this method as a raw material.

The inventor of the present invention has found that, when the placenta is fermented with lactic acid bacteria or the like, the amino acid content is higher and the placenta's physiological activity is higher than that of the placenta extract obtained by the conventional method, unlike the conventional method of obtaining the placenta extract, And thus the present invention has been completed.

It is an object of the present invention to provide a method for producing a fermented placental composition.

Another object of the present invention is to provide a fermented placenta composition obtained by the above method.

It is still another object of the present invention to provide an atopic dermatitis remedy composition and an anti-stress agent composition using the fermented placental composition.

In one aspect, the invention relates to a method of making a fermented placental composition.

The method for preparing a fermented placenta composition of the present invention comprises the steps of (a) pulverizing a mammalian placenta, (b) adding a carbon source and an energy source of the microorganism inoculated to the placenta pulverized product and mixing them, and (c) , Yeast, and microorganisms of the genus Bacillus, and fermenting the microorganism.

As shown in the following Examples and Experimental Examples, when the fermented product obtained by inoculating lactic acid bacteria, yeast and / or Bacillus sp. Microorganisms into the placenta pulverized product regardless of the type of placenta, the placenta obtained by treating the protease in the amino acid content, It was about 1.5 higher than the extract, regardless of the amino acid.

In addition, the physiological activity was significantly higher than that of the placenta extract obtained by treating with proteolytic enzyme, as seen through improvement activity of atopic dermatitis and antistress activity.

Particularly, the fermented placental composition thus obtained had little disgusting peculiar to the placenta, and when the lactic acid bacteria were used in the fermentation process, the pH of the fermented placenta composition was about 3.0, so that it could be stored at room temperature for a long time without adding preservatives. Placenta extract obtained by hydrolysis such as enzymatic treatment) and the like. Conventional placenta raw materials have a limitation in raising the solid content of placenta raw materials (usually produced at a level of 3 to 5%) in beverage production due to the disgusting characteristic of the placenta.

Terms not specifically defined in this specification follow conventional meanings in the art.

In the method for producing the fermented placenta composition of the present invention, the placenta that can be used in step (a) is a placenta of all mammals, and even if the placenta, the equine placenta and the placenta are used in the following examples of the present invention But is not limited thereto. Since a person of ordinary skill in the art has no difficulty in applying the method of the present invention to the placenta of other mammals.

The placenta of the mammal used in the method of the present invention will usually be used in the pulverization. The particle diameter of the pulverized product is not particularly limited, but is preferably such that the pulverized product is uniformly fermented by the inoculated microorganism. Here, the particle diameter refers to the length in the longitudinal direction of the pulverized material particles, and the particle diameter to such an extent that uniformly fermentable by the inoculated microorganism can be determined within a range of ordinary skill in the art. In the following examples of the present invention, pulverization was used in a size of 0.3-0.8 mm. Since the mammalian placenta usually has a moisture content of about 90%, it is not necessary to supply moisture specifically for fermentation. However, when the mammalian placenta is powdered by freeze-drying or the like and used as a raw material for fermentation, Will be.

 In addition, in the method for producing the fermented placenta composition of the present invention, the carbon source and the energy source of the inoculated microorganism in the step (b) are well known in the art and thus any known in the art can be used, , Lactose, glucose, fructose, sugar, mixtures thereof, and the like. These carbon sources and energy sources can be added in an arbitrary range in consideration of the intended fermentation time and degree of fermentation. Usually in the range of 3 to 30 parts by weight based on 100 parts by weight of the placental pulverized product.

There is no particular limitation on lactic acid bacteria, yeast, and Bacillus sp. Microorganisms that can be used in the method of the present invention.

In the case of lactic acid bacteria, lactic acid bacteria such as Lactobacillus sp. , Streptococcus sp. , Pediococcus sp. , Leuconostoc sp. And Bifidobacterium sp. ( Bifidobacterium sp. ) . All of these lactic acid bacteria can be used in the method of the present invention. Specific examples thereof include Lactobacillus plantarum , Lactobacillus delbrueckii , Lactobacillus bulgaricus , Lactobacillus casei , Lactobacillus brevis , Lactobacillus brevis , But are not limited to, Lactobacillus acidophilus , Pediococcus pentosaceus , Pediococcus cerevisiae , Lactococcus lactis , Leuconostoc citreum , Leuconostoc mesenteroides , Bifidobacterium bifidum , and the like can all be used in the method of the present invention.

In the case of yeast, the genus Saccharomyces Yeasts are preferable. Examples of such yeasts include Saccharomyces rouxii , Saccharomyces cereviciae , Saccharomyces oviformis , Saccharomyces stainerii , ( Saccharomyces steineri ) and the like.

As the Bacillus sp. Which can be used in the method of the present invention, any microorganisms involved in the fermentation of food such as Chungkukjang, doenjang, and cheese can be used. Specifically, Bacillus subtilis (B. subtilis), Bacillus leakage four formate miss (B. lichneformis), Bacillus MEGATHERIUM (B. megaterium), Bacillus amyl Lowry Quebec Pacific Enschede (B. amyloliquefaciens), Bacillus natto (B . natto), and the like are typically, in addition to Bacillus la Alliance system (B.antharcis), Bacillus alkylene tooth (B.lentus), Bacillus mirror's buffer (B.pumilus), Bacillus rendering if N-Sys (B.thuringiensis), Bacillus al B. alvei , B. azotophixans , B. maesterans , B. polymyxa , B. popilliae , Bacillus coagulans ( B ), Bacillus subtilis, Bacillus subtilis, .coagulans), Bacillus stearate loader's parent filler (B.stearothermophilus), Bacillus Pas toeri (B.pasteurii), Bacillus's are also the present invention including coarse Parry (B.sphaericus), Bacillus Pastes video suspension (B.fastidiosus) Can be used.

In the method of the present invention, it is preferable that the fermentation of the step (c) is carried out until the inoculated microorganism reaches the maximum growth. The time point at which the inoculated microorganism reaches its maximum is the fermentation temperature, the amount of the fermentation raw material (mixture of the fermentation product and the carbon source and energy source of the microorganism as the mixture of step (b)), Will be determined accordingly. Those skilled in the art will be able to determine at what point in time the maximum growth of the inoculated microorganisms takes into account, in consideration of the fermentation temperature, the amount of the fermentation raw material, the amount of the microorganism to be inoculated, and the like within its normal capability. According to the present inventors' experience, when the fermentation temperature is 38 ° C, the placenta pulverized product is 2000 g, the sugar is 200 g, and the oligosaccharide is 300 g, when the microorganism is inoculated with 3% (w / v) It took 10 days until the maximum growth of the inoculated microorganism was reached.

In the method of the present invention, the fermentation temperature of step (c) may be in the range of 15 ° C to 45 ° C. Preferably in the range of 20 [deg.] C to 40 [deg.] C. If the fermentation temperature is lower than the above range, the fermentation rate may be slowed down and a desired fermentation product may be produced. Even if the fermentation temperature is higher than the above range, the fermentation rate may be slowed down or the desired fermentation product may be produced have.

Further, in the method of the present invention, an aging step may be further included after the step (c). This aging step is intended to stabilize the fermentation product by the microorganism even after the inoculated microorganism stops growing, and this aging step may be performed for about 10 to 12 months.

Further, in the method of the present invention, a sterilization step may be further included after the step (c). This sterilization step can be carried out using conventional methods known in the art such as heat treatment, ultraviolet radiation, and the like.

In another aspect, the present invention relates to a fermented placental composition obtained by the method as described above.

The fermented placental composition of the present invention may be initially obtained in the form of a liquid, but it may be concentrated or powdered by methods such as carcinogenesis, lyophilization and the like. It may be precipitated with an appropriate precipitant such as ethanol or acetone to remove the supernatant and recover the precipitate.

As described above, the fermented placenta composition of the present invention has higher amino acid content than conventional placental raw materials obtained by enzymatic treatment and the like, higher physiological activities such as atopic dermatitis improving activity and antistress activity than the conventional placental raw materials, It is characterized by almost no disgusting odor. Therefore, the fermented placenta composition of the present invention is suitable not only for use as a raw material for cosmetics but also for use as a food raw material for beverages and the like.

In another aspect, the present invention relates to an atopic dermatitis remedy composition comprising the fermented placenta composition as described above as an active ingredient.

As used herein, "improvement" is meant to include treatment, prevention, or alleviation of symptoms.

Also, in the present specification, the above-mentioned "active ingredient" means a component that alone exhibits the desired activity or can exhibit activity together with a carrier that is itself inactive.

Meanwhile, the composition for improving atopic dermatitis of the present invention may contain any amount (effective amount) of the fermented placenta composition of the present invention, which is an effective ingredient thereof, as long as it can exhibit atopicidal activity according to the properties, uses, formulations, , A typical effective amount will be determined within the range of from 0.001% to 15% by weight based on the total weight of the composition. Herein, "effective amount" refers to the amount of active ingredient capable of inducing treatment, prevention, or alleviation of symptoms of atopic dermatitis. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

In another aspect, the present invention relates to an anti-stress agent composition comprising the fermented placental composition as described above as an active ingredient.

In general, when a stimulus on a living body exceeds a certain level, the stimulus induces various negative responses of the body, which is defined as stress (Selye, H.: The stress of life, Toronto, Longnans Green and Co., pp. 1-50, 1958). Examples of the body reaction caused by stress include tension headache, migraine, hypertension, indigestion, fatigue, pain, insomnia, menopause, roughness of the skin, gastric ulcer, and decreased immunity of the body.

The following experimental results show that more than 80% of the participants have improved fatigue, insomnia, headache and skin roughness as a result of clinical evaluation of beverages containing the fermented placenta composition of the present invention.

It can be said that this can be effectively used as antistress agent of the fermented placenta composition of the present invention.

As used herein, the term " anti-stress "means a stress-induced physical response, such as a tension headache, migraine, hypertension, indigestion, fatigue, pain, insomnia, menopause, skin roughness, gastric ulcer, and / Treatment, and / or alleviation of symptoms.

The atopic dermatitis remedy composition and the anti-stress composition (hereinafter, the composition of the present invention) of the present invention can be used as a pharmaceutical composition in a specific embodiment.

The pharmaceutical composition of the present invention may be in the form of oral dosage forms (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (including sterile injectable aqueous (Oil, cream, ointment, gel, lotion, patch), and the like.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity (sufficiently low toxicity) without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (e.g. corn starch, potato starch, etc.), cellulose, derivatives thereof (e.g. sodium carboxymethylcellulose, ethylcellulose, etc.) malt, gelatin, talc, (E.g., peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyol (e.g., propylene glycol, glycerin and the like), alginic acid, emulsifiers (e.g., TWEENS), humectants Such as sodium lauryl sulfate, a coloring agent, a flavoring agent, a tableting agent, a stabilizer, an antioxidant, a preservative, water, a saline solution, and a phosphate buffer solution. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, when the pharmaceutical composition of the present invention is prepared by an aqueous suspension, suitable excipients include sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose , Sodium alginate, polyvinylpyrrolidone, and the like. Suitable excipients when prepared from injection solutions include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in some cases, can be administered topically.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

In another specific embodiment, the composition of the present invention can be used as a food composition.

The food composition of the present invention may be manufactured from gums, a vitamin complex, a health supplement, a nutritional supplement food, a functional drink, and the like.

The food composition of the present invention includes not only its effective component but also a sweetener such as corn syrup solids, honey, sucrose, fructose, lactose and maltose, fruits such as apple, lemon and citrus fruits, green tea leaves, A physiologically active ingredient such as flavorings, catechins, retinol, ascorbic acid and tocopherol obtained in the tea, and minerals such as calcium, magnesium, chromium, cobalt, copper and fluoride can be further added. When other food additives are added It is acceptable.

In another specific embodiment, the composition of the present invention can be identified as a cosmetic composition.

When the composition of the present invention is recognized as a cosmetic composition, the cosmetic composition may be prepared in various forms, for example, emulsion, lotion, cream (oil-in-water type, oil water type, multiphase), solution, suspension Water, water), anhydrous products (oil and glycol), gels, masks, packs or powders.

The composition of the present invention may contain an acceptable carrier in cosmetic preparations in addition to its active ingredients.

As used herein, the term " acceptable carrier for cosmetic preparation "refers to a compound or composition which is already known and used in cosmetic preparations, or which is a compound or composition to be developed in the future and which is toxic, instable or irritating It says nothing.

The carrier may be included in the skin whitening cosmetic composition of the present invention in an amount of about 1% by weight to about 99.99% by weight, preferably about 50% by weight to about 99% by weight of the composition, based on the total weight thereof.

However, since the above ratio depends on the above-described form of the cosmetic product of the present invention and its specific application area (face or hands) or its preferable application amount, the ratio is in any aspect the range of the present invention Should not be construed as limiting.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, humectants, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents and perfumes.

The compounds / compositions which can be used as the carrier and which can be used as alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosifiers, emulsions, stabilizers, sunscreens, A person skilled in the art can select and use appropriate substances / compositions.

As described above, according to the present invention, a fermented placenta composition having high amino acid content, high physiological activity, little placenta-specific disgusting odor, and a preparation method thereof can be provided.

The fermented placenta composition of the present invention is useful as a raw material for cosmetics, foods and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

EXAMPLE < RTI ID = 0.0 > Preparation of fermented placenta composition

Example 1 Preparation of Fermented Porcine Placenta Composition

<Example 1-1> Preparation of fermented porcine placenta composition Example 1

The frozen porcine placenta was thawed and ground to a size of 0.3-0.8 mm. 200g of placental pulverization liquid, 200g of sugar, and 300g of oligosaccharide were mixed and placed in a container. The microorganisms inoculated at the time of fermentation were inoculated with 3% (w / v) of a mixture of Lactobacillus plantarum and Saccharomyces cereviciae (mixed at a weight ratio of 1: 1) Lt; 0 &gt; C for 20 days. After culturing, the culture solution was sterilized by heating at 90 to 100 ° C for 1 hour, and then filtered with a Wassmann paper filter paper (Whatman No. 2) to obtain a fermented placenta composition.

<Example 1-2> Preparation of fermented porcine placenta composition Example 2

The fermented placenta composition was prepared by the same process and conditions as in < Example 1 > except for the inoculating microorganism. The microorganisms inoculated here are 3% (w / v) of a culture mixture of Bacillus subtilis and Saccharomyces cerevisiae (mixed at a weight ratio of 1: 1).

<Example 1-3> Preparation of fermented porcine placenta composition Example 3

The fermented placenta composition was prepared by the same process and conditions as in < Example 1 > except for the inoculating microorganism. The inoculated microorganism is 3% (w / v) of a mixture of Bacillus subtilis and Lactobacillus plantarum culture (mixed at a weight ratio of 1: 1).

<Example 1-4> Preparation of fermented porcine placenta composition Example 4

The fermented placenta composition was prepared by the same process and conditions as in < Example 1 > except for the inoculating microorganism. The inoculated microorganism was cultivated in a mixture ratio of Bacillus subtilis , Lactobacillus plantarum and Saccharomyces cereviciae (1: 1: 1) 3% (w / v).

Example 2: Preparation of a placenta composition after fermentation

&Lt; Example 2-1 > Preparation Example 1 of a placenta composition after fermentation

The fermentation was carried out in the same manner as in <Example 1-1> except that a horse placenta was used instead of the porcine placenta.

&Lt; Example 2-2 > Preparation Example 2 of a placenta composition after fermentation

The fermentation was carried out in the same manner as in <Example 1-2>, except that a horse placenta was used instead of the porcine placenta.

&Lt; Example 2-3 > Preparation Example 3 of a placenta composition after fermentation

The fermentation was carried out in the same manner as in <Example 1-3>, except that horse placenta was used instead of porcine placenta.

&Lt; Example 2-4 > Preparation Example 4 of Placenta composition after fermentation

The fermentation was carried out in the same manner as in <Example 1-4>, except that a horse placenta was used instead of a porcine placenta.

&Lt; Example 3 > Preparation of fermented sheep placenta composition

&Lt; Example 3-1 > Preparation Example 1 of fermented sheep placenta composition

The same fermentation was carried out as in <Example 1-1> except that placenta of both placenta was used instead of porcine placenta.

&Lt; Example 3-2 > Preparation Example 2 of fermented sheep placenta composition

The fermentation was carried out in the same manner as in <Example 1-2>, except that placenta of both placenta was used instead of porcine placenta.

Example 3-3 Preparation of fermented sheep placenta composition Example 3

The same fermentation as in <Example 1-3> was used except that placenta of both placenta was used instead of porcine placenta.

Example 3-4 Preparation of fermented sheep placenta composition Example 4

The fermentation was carried out in the same manner as in <Example 1-4>, except that placenta of both placenta was used instead of porcine placenta.

&Lt; Comparative Example > Preparation of placenta extract treated with enzyme

&Lt; Comparative Example 1 > Preparation Example 1 of enzyme-treated porcine placenta extract

The frozen placenta was thawed and ground to a size of 0.3-0.8 mm. Then, 30 g of protease (product name: "alcalase", Novozyme) was added to 2,000 g of placenta and allowed to react at 60 ° C. for 5 hours. For 30 minutes to stop the reaction. The following reaction mixture was filtered with Wassmann paper filter paper (Whatman No. 2), and then the enzyme-treated porcine placenta extract was obtained.

&Lt; Comparative Example 2 > Preparation of an enzyme-treated horse placenta extract Example 2

An enzyme-treated horse placenta extract was prepared in the same manner as in <Comparative Example 1> except that a horse placenta was used instead of the porcine placenta.

&Lt; Comparative Example 3 > Preparation Example 3 of enzyme-treated placenta extract

An enzyme-treated placenta extract was prepared in the same manner as in <Comparative Example 1> except that a placenta of both placenta was used instead of the porcine placenta.

<Preparation Example> Preparation of skin lotion and beverage using fermented placenta composition

&Lt; Preparation Examples 1 to 3 > Preparation of skin lotion using fermented placenta composition

The fermented porcine placenta composition of Example 1-4, the fermented late-stage placenta composition of Example 2-4, and the fermented placenta composition of Example 3-4 were used to prepare the skin Lotion was prepared.

The placental composition has a pH of about 3.5, regardless of the type of placenta in which the pH is used. The pH of the solution was adjusted to 5.5 with sodium hydroxide (NaOH) or calcium carbonate (CaCO ₃ ), and the solution was concentrated and freeze-dried to prepare a fermented placenta extract powder. Then, 2.5 g of the powder was dissolved in 96.3 g of purified water , A skin lotion having a solid content of 2.5% was prepared by mixing 0.2 g of a natural flavor (e.g., lavender essence oil), 0.8 g of an emulsifier (e.g. HC60 and the like) and 0.2 g of a preservative (e.g.

All of the lotions thus obtained were transparent, brownish in shape, and had no odor. The pH was about 5.5 and slightly acidic.

Herein, the skin lotions each containing the fermented placenta compositions of Examples 1-4, 2-4 and 3-4 are referred to as Preparation Examples 1 to 3, respectively.

&Lt; Preparation Examples 4 to 6 > Functional drinks using extracts of fermented placenta compositions

Using the fermented porcine placenta composition of Example 1-4, the fermented hatching placenta composition of Example 2-4, and the fermented sheep placenta composition of Example 3-4, A beverage was prepared.

Fermented Placenta Extract After mixing 4.0 g of the dried powder composition, 95.5 g of jujube and licorice mixed extract (5.0% solids) and 0.5 g of grapefruit seed extract, which is a commercially available natural preservative, and mixing, The beverage was packed to prepare a beverage.

Here, the beverages containing the fermented placenta compositions of Examples 1-4, 2-4 and 3-4 are referred to as Preparation Examples 4 to 6, respectively.

&Lt; Experimental Example > Component analysis and functional evaluation of fermented placenta composition

<Experimental Example 1> Analysis of composition of fermented placenta composition

The fermented porcine placenta composition of Example 1-4, the fermented hatching placenta composition of Example 2-4, the fermented sheep placenta composition of Example 3-4, and each comparative example The components of placenta extract were compared and analyzed.

The components were analyzed with Sykam amino acid analyzer (Sykam, Germany).

The results are shown in Tables 1 to 3.

Placenta component analysis results Item The placenta extract of Comparative Example 1 <Example 1-4> Fermented porcine placenta composition Total nitrogen 13.0% 14.4% protein 90.2% 90.5% Ash 4.7% 3.8% General bacterial count 120 g or less Non-detection L-Asparagine 4.2 g / 100 g 6.0 g / 100 g L-glutamine 5.6 g / 100 g 8.1 g / 100 g L-arginine 4.0 g / 100 g 4.9 g / 100 g L-proline 5.3 g / 100 g 6.8 g / 100 g L-tyrosine 0.9 g / 100 g 1.4 g / 100 g L-leucine 3.2 g / 100 g 5.1 g / 100 g

&Lt; Experimental Example 2 > Evaluation of activity for improving atopic dermatitis

end. Selection of target group

Twenty patients with atopic dermatitis who were treated only with atopic dermatitis moisturizing cosmetics and received acupuncture treatment at the oriental clinic but did not use medicines such as dermatology for the treatment of atopic dermatitis were selected within the past month .

I. Test method

The skin lotions prepared in the above Preparation Examples 1 to 4 were divided into morning and evening two times a day, so that they could be applied once more only in a severe itchy condition. During the test period, the use or application of other therapeutic agents for atopic dermatitis was prohibited during the treatment period.

All. result

The test was performed for 4 weeks and the degree of improvement in percentage of change in EASI score is shown in Table 2 below. Atopic dermatitis index is classified into four stages of nocturnal, mild, moderate, and severe degree of erythema, edema, swelling, palsy, and burning in the head, neck, upper body, torso, And the sum of the area scores indicates the degree of skin lesion objectively. The higher the value, the more severe the atopic symptoms, and the lower the better, the better the skin improvement effect.

EASIscore change percentage = (first EASIscore-4 weeks EASIscore) / first EASIscore X100

On the other hand, as a skin lotion of the comparative example, 2.5 g of the powdery extract obtained by lyophilizing the placenta extract of each of the comparative examples was dissolved in 96.3 g of purified water, and then 0.2 g of a natural flavor (for example, lavender essence oil) Etc.), 0.2 g of a preservative (for example, complex paraben etc.), and the like were used as a skin lotion having a solid content of 2.5%.

Percent change in atopy severity index (EASIscore) Test Products Percentage change in EASI score (%) Lotion of Preparation Example 1 85.8% Lotion of Preparation Example 2 79.2% Lotion of Production Example 3 82.7% Lotion of Production Example 4 82.3% The lotion of Comparative Example 1 51.0% The lotion of Comparative Example 2 62.3% Lotion of Comparative Example 3 54.0%

Table 2 shows that the skin lotion containing the fermented placenta composition of the present invention has a higher atopic dermatitis improving activity than the lotion containing the placenta extract of the comparative example regardless of the type of the placenta.

For reference, a photograph showing the degree of improvement of atopic dermatitis when the lotion of <Preparation Example 1>, which has the most excellent effect, is used is shown in FIG. In Fig. 1, the photograph on the left is the pre-treatment photograph, and the photograph on the right is the post-treatment treatment for 4 weeks.

For reference, the results of the questionnaire survey of 5 persons using lotion of the above <Preparation Example 1> are shown in <Table 3>.

Summary of Questionnaire Evaluation Using Skin Lotion of <Manufacturing Example 1> Age and gender Results of 5 questionnaires using lotion of <Preparation Example 4>
Of 3, M (1) Main symptoms: neck keratinization and itching
(2) Use: once a day
(3) Treatment progress: Itching significantly suppressed from day 7
Second one, M (1) Main symptoms: inflammation and itching of folds such as arms, legs and neck
(2) Use: twice a day
(3) Treatment progress: Itching suppression from the first day after use and the inflamed area on the 7th day began to disappear almost on the 14th day
Second 2, M (1) Main symptoms: Inflammation and itching of folds such as arms, legs and neck
(2) Use: twice a day
(3) Treatment progress: inflammation and itching disappear 14 days later
High 3, F ( 1) Main symptoms: reddened both sides of the face
(2) Use: twice a day
(3) Efficacy: It began to improve from the second day, and from the 15th day, the inflammation site of the face except for acne began to soot
Second 6, F (1) Main symptoms: systemic inflammation and itching
(2) Use: twice a day
(3) Efficacy: itching does not settle, skin inflammation site keeps constant,

As shown in <Table 3>, among the five experimental subjects, the effect of atopic dermatitis soothing was not significant in the case of the girl who reached adolescence (6th grade). In the remaining 3 students and 1 high school student, itching inhibition and inflammation So that it showed excellent efficacy.

<Experimental Example 3> Anti-stress and skin improvement test

In order to understand the effects of complex mental stress and thus subjective physical changes (eg, fatigue, insomnia, headache, skin roughness, etc.) (100ml capacity) were supplied per person. One bottle was drunk on an empty stomach during the day and was performed for 30 days.

On the other hand, 4.0 g of the powdery composition obtained by lyophilizing the placenta extract of each of the comparative examples, 95.5 g of jujube and licorice mixed extract (solid content 5.0%), and 0.5 g of a grapefruit seed extract as a commercially available natural preservative The mixture was put into a heat-resistant PE container while keeping the product temperature at 90 ° C, and packed to prepare a beverage.

As a general consumer evaluation after the test, the degree of preference of the beverage according to the embodiment of the present invention was higher than that of the fermented placenta composition of the present invention. The body was refreshing and refreshing in about 30 minutes. Enzyme treatment of the comparative example In the beverage containing the placenta extract composition, it was found that some specific odor remained in the mouth after drinking, and no unusual feeling appeared immediately after ingestion.

The detailed test results are shown in the following Tables 4 to 9, and the fermented placenta composition as a whole showed a relatively high antistress effect as compared with the enzyme treated placenta extract. These effects were relatively good in recovering fatigue, and the skin roughness was improved relatively quickly with the relief of insomnia. These characteristics can be similarly compared with soybean paste, soybean milk, yogurt, etc., and even when the same raw materials are used, they are more readily absorbed by fermentation, and the increase of natural microbial cells due to fermentation microorganism growth, (Increased antioxidant activity) of the placenta.

The anti-stress effect of <Preparation Example 4> Number of participants Excellent effect Somewhat effective no effect Fatigue 6 2 3 One Blow 3 2 One 0 headache 3 0 2 One Skin rough 3 2 One 0 * There is no effect of 50 points with improvement score of 100 points, 50 points with a little effect of 80 points, and 90 points or more shows excellent effect.

Antistress effect of < Production Example 5 > Number of participants Excellent effect Somewhat effective no effect Fatigue 6 3 3 0 Blow 3 One 2 0 headache 3 One One One Skin rough 3 2 One 0 * There is no effect of 50 points with improvement score of 100 points, 50 points with a little effect of 80 points, and 90 points or more shows excellent effect.

Antistress effect of < Production Example 6 > Number of participants Excellent effect Somewhat effective no effect Fatigue 6 2 3 One Blow 3 2 One 0 headache 3 One One One Skin rough 3 One 2 0 * There is no effect of 50 points on the improvement scale of 100 points, 50 points -80 points is effective somewhat, and 90 points or more is effective.

The anti-stress effect of Comparative Example 1 Number of participants Excellent effect Somewhat effective no effect Fatigue 6 One 4 One Blow 3 One 2 0 headache 3 0 2 One Skin rough 3 One 2 0 * There is no effect of 50 points with improvement score of 100 points, 50 points with a little effect of 80 points, and 90 points or more shows excellent effect.

The anti-stress effect of Comparative Example 2 Number of participants Excellent effect Somewhat effective no effect Fatigue 6 2 3 One Blow 3 One One One headache 3 0 2 One Skin rough 3 2 One 0 * There is no effect of 50 points with improvement score of 100 points, 50 points with a little effect of 80 points, and 90 points or more shows excellent effect.

The anti-stress effect of Comparative Example 3 Number of participants Excellent effect Somewhat effective no effect Fatigue 6 0 5 One Blow 3 One One One headache 3 0 2 One Skin rough 3 One One One * There is no effect of 50 points with improvement score of 100 points, 50 points with a little effect of 80 points, and 90 points or more shows excellent effect.

Fig. 1 is a photograph showing the degree of improvement of atopic dermatitis when skin lotion containing the fermented placenta composition of the present invention is used for 4 weeks. Fig. A: Before use, B: After use

Claims (18)

(a) pulverizing the mammalian placenta, (b) adding and mixing the carbon source and the energy source of the microorganism inoculated into the placenta pulverized product, and (c) inoculating the mixture with at least one microorganism selected from the group consisting of lactic acid bacteria, yeast and microorganisms of the genus Bacillus, and fermenting A method for preparing a fermented placenta composition. The method according to claim 1, The carbon source and the energy source of the inoculated microorganism in step (b) are oligosaccharide, lactose, glucose, fructose, sugar or a mixture thereof, and the amount thereof is 3 parts by weight to 30 parts by weight based on 100 parts by weight of the placental pulverized product To a fermented placenta composition. The method according to claim 1, The lactic acid bacteria are preferably selected from the group consisting of Lactobacillus sp. , Streptococcus sp. , Pediococcus sp. , Leuconostoc sp. , And Bifidobacterium sp. Wherein the lactic acid bacteria are selected from the group consisting of Bifidobacterium sp . The method according to claim 1, The lactic acid bacteria are preferably selected from the group consisting of Lactobacillus plantarum , Lactobacillus delbrueckii , Lactobacillus bulgaricus , Lactobacillus casei , Lactobacillus brevis , Lactobacillus brevis , But are not limited to, Lactobacillus acidophilus , Pediococcus pentosaceus , Pediococcus cerevisiae , Lactococcus lactis , Leuconostoc citreum , Wherein the lactic acid bacteria are selected from the group consisting of Leuconostoc mesenteroides and Bifidobacterium bifidum . The method according to claim 1, The yeast is selected from the group consisting of Saccharomyces sp. Wherein the fermented placenta is a yeast. The method according to claim 1, The yeast may be selected from the group consisting of Saccharomyces rouxii , Saccharomyces cereviciae , Saccharomyces oviformis , and Saccharomyces steineri . &Lt; / RTI &gt; wherein the yeast is selected from the group consisting of: &lt; RTI ID = 0.0 &gt; The method according to claim 1, It said Russ baril in microorganisms (Bacillus sp.) Is Bacillus subtilis (B. subtilis), Bacillus leak four FORT Miss (B. lichneformis), Bacillus MEGATHERIUM (B. megaterium), Bacillus amyl Laurier Quebec Pacifico Enschede (B amyloliquefaciens , and B. natto . In addition, there are B. anthracis , B. lentus , B. pumilus , Bacillus thuringiensis, (B.thuringiensis), Bacillus al bay (B.alvei), Bacillus azo topic Sans (B.azotofixans), Bacillus maese lance (B.macerans), Bacillus Poly miksa (B.polymyxa), Bacillus papil Rie (B.popilliae ), Bacillus core raglan switch (B.coagulans), Bacillus stearate loader Russ a brush (B.stearothermophilus), Bacillus Pas toeri (B.pasteurii), Bacillus Parry's carcass (B.sphaericus) and Bacillus Pastes video suspension (B. fastidiosus ) Wherein the fermented placenta composition is a microorganism. The method of claim 1, wherein Wherein the fermentation of step (c) is performed at a temperature ranging from 20 ° C to 40 ° C. The method according to claim 1, Wherein the fermented placenta composition further comprises an aging step after step (c). The method according to claim 1, Wherein the fermented placenta composition further comprises a sterilization step after step (c). delete A composition for improving atopic dermatitis, comprising the fermented placental composition obtained by the method according to any one of claims 1 to 10 as an active ingredient. 13. The method of claim 12, Wherein the composition is a cosmetic composition. 13. The method of claim 12, The composition for improving atopic dermatitis according to claim 1, wherein the composition is a pharmaceutical composition. An anti-stress agent composition comprising the fermented placental composition obtained by the method according to any one of claims 1 to 10 as an active ingredient. delete delete delete

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