KR102589579B1 - Functional food for preventing and improving female menopausal symptoms using horse placenta - Google Patents

Abstract

The present invention relates to a functional food for preventing and improving female menopausal symptoms using horse placenta.
A functional food for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention contains hydrolyzed and fermented horse placenta as an active ingredient, and the hydrolyzed and fermented horse placenta contains the total content of the functional food. It may be included in 1 to 10% by weight.
Functional food for preventing and improving female menopausal symptoms using horse placenta according to various embodiments of the technical idea of the present invention with the above configuration, produces hydrolyzed fermented product by hydrolyzing horse placenta and using it in food, thereby causing side effects. Even without this, it is effective in preventing and improving female menopausal symptoms.

Description

Functional food for preventing and improving female menopausal symptoms using horse placenta {FUNCTIONAL FOOD FOR PREVENTING AND IMPROVING FEMALE MENOPAUSAL SYMPTOMS USING HORSE PLACENTA}

The present invention relates to a functional food for preventing and improving female menopausal symptoms using horse placenta. More specifically, it relates to a functional food for preventing and improving female menopausal symptoms by hydrolyzing horse placenta to produce a hydrolyzed fermented product and using it in food to relieve female menopausal symptoms without causing side effects. This is about a functional food for preventing and improving female menopausal symptoms using horse placenta, which has excellent prevention and improvement effects.

Due to modern industrialization, the economic level has improved and medical science has developed, extending human lifespan and increasing the elderly population. Therefore, research is being conducted to improve the health and quality of life of the elderly population.

In particular, women are experiencing physical and mental difficulties due to menopausal symptoms that gradually begin after their 40s. Menopause, experienced by about 20% of all women, is one of the aging phenomena that begins gradually from the age of 40. However, as the average lifespan is extended, it accounts for more than 1/3 of women's lives.

Female menopause is a type of endocrine syndrome and refers to a transitional period in which physiological and sexual functions are reduced or lost due to a decrease in female hormones, that is, estrogen, due to the overall and gradual aging of ovarian function. Estrogen exerts essential regulatory effects such as catabolism and activation in the female uterus, vagina, skeletal muscle, and cardiovascular system (Couse and Korach, 1999; Korach et al, 1995), as well as proper ovulation, fertilization of eggs, and pregnancy. It is known to play a role in regulating bone structure and cholesterol, and is also applied to the evaluation of ovarian function.

Symptoms of menopause include symptoms due to vascular changes such as hot flashes, tachycardia, sweating or headaches, symptoms due to musculoskeletal changes such as muscle pain, joint pain and back pain, symptoms due to urogenital changes such as frequent urination or urinary incontinence, and atrophy of the uterus and vagina. General symptoms such as memory loss, depression, loss of concentration, and dizziness due to changes in the brain nervous system, loss of vision, and changes in skin and hair are known. It is being noted that these important changes that affect women's life and health occur in 80% of pre- and post-menopausal women and progress over a long period of time, increasing the risk of developing diseases that are fatal to women's health, such as osteoporosis and cardiovascular disease. (Turner et al, 1994; Versi et al, 2001).

Therefore, there is a need to develop treatments that can improve menopausal symptoms in order to improve the physical and mental health and quality of life of middle-aged women. Examples of treatments that can improve menopausal symptoms include drug therapy using drugs such as hormone replacement therapy or non-steroidal agents. However, most drugs, including non-steroidal drugs, are known to have side effects such as headaches and weight gain. In addition, it is known that replacement therapy using hormones such as estrogen may increase the risk of rejection, uterine bleeding, stroke, heart attack, breast cancer, and uterine cancer due to the administration of artificial hormones into the body (Swaran L, et al , Obstetrics & Gynecology, 91, 678-684, 1998).

Meanwhile, the placenta is an organ that forms in the uterus of a pregnant mammal and supplies nutrients to the fetus through the umbilical cord and sustains its life. The placenta is expelled from the uterus during childbirth, and the coming out of the placenta is called postpartum.

The placenta contains essential amino acids, melatonin, nucleic acid components such as RNA and DNA, the antioxidant enzyme SOD (Super Oxide Dismutase), hyaluronic acid, antioxidants, immune cofactors (cytocine), placental peptides, and insulin-like growth promoting factors ( Contains growth factors and cytokines such as insulin-like-growth factor (EGFS), Epidermal Growth factors (EGFS), and unique Senescent Cell Adivating Factors (SCAFS) to help relieve fatigue and enhance immunity. It is known to be useful, etc.

In addition, the placenta has the effect of inhibiting the activity of the enzyme tyrosinase, which promotes the production of melanin granules from tyrosine, thereby inhibiting pigment formation and at the same time promoting the discharge of cells containing melanin pigment, preventing and improving melasma. It is effective in preventing pigmentation and ultimately has a whitening effect as well. (Mallick S et el., Pigment Cell Res. Feb 18(1), 25-33, 2005).

In addition, the tissue metabolism promoting effect of the placenta increases the function of the sebum secretion glands and purifies the secretion of sebum, thereby reducing or eliminating fine wrinkles. In addition, the placenta contains fibroblast growth factor (FGF), which promotes the proliferation and regeneration of fibroblasts, and through its activation, increases the amount of collagen, elastin, and hyaluronic acid, restoring elasticity and elasticity of the skin, thereby restoring the elasticity and elasticity of the skin. It works to improve wrinkles and sagging skin.

As a method of extracting the placenta, a method of hydrolyzing the placenta using a strong acid (usually 3N hydrochloric acid) or a strong alkali to obtain the product has been used. However, in this method, various amino acids, etc. contained in the placenta are removed in the process of high temperature treatment for a long time. There is a problem that bioactive substances are destroyed. In addition, the conventional method of extracting the placenta using enzymes has the problem that it takes a long time to extract the active ingredient, so there is a need to develop a method to improve this.

In addition, due to the unpleasant odor and dark color when the placenta is hydrolyzed, it is difficult to apply it to food, so an extremely small amount is used as a raw material.

Domestic Registered Patent No. 10-2049440 (registered on November 21, 2019) Domestic Registered Patent No. 10-1906720 (registered on October 2, 2018) Domestic Registered Patent No. 10-1544532 (registered on August 7, 2015)

The problem that the present invention aims to solve is to prevent female menopause symptoms using horse placenta, which has no side effects and is excellent for preventing and improving female menopausal symptoms by hydrolyzing horse placenta to produce hydrolyzed fermented product and using it in food. The goal is to provide functional foods for improvement.

The various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

In one embodiment of the technical idea of the present invention, a functional food for preventing and improving female menopausal symptoms using horse placenta is disclosed.

The functional food for preventing and improving female menopausal symptoms using the horse placenta contains hydrolyzed and fermented horse placenta as an active ingredient, and the hydrolyzed and fermented horse placenta may be included in 1 to 10% by weight of the total content of the functional food. .

Specific details of other embodiments are included in the detailed description.

Functional food for preventing and improving female menopausal symptoms using horse placenta according to various embodiments of the technical idea of the present invention is made by hydrolyzing horse placenta to produce a hydrolyzed fermented product and using it in food, thereby preventing female menopause without causing side effects. It is effective in preventing and improving symptoms.

It will be fully understood that various embodiments of the technical idea of the present invention can provide various effects that are not specifically mentioned.

Figure 1 is a flowchart schematically illustrating a method for producing hydrolyzed fermented horse placenta used in functional foods for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention.
Figure 2 is a graph showing the results of analyzing the effect of hydrolyzed fermented horse placenta on blood estrogen concentration using female menopausal disease model rats.
Figure 3 is a graph showing the results of analyzing the effect of hydrolyzed fermented horse placenta on osteoporosis caused by female menopausal disease using female menopausal disease model rats.
Figure 4 is a graph showing the toxicity of the hydrolyzed fermented horse placenta prepared according to the example to osteoblasts.

The advantages and features of the present invention, and methods for achieving them, will become clear with reference to the embodiments described in detail below. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosure will be thorough and complete and so that the spirit of the invention can be sufficiently conveyed to those skilled in the art.

The terms used in this application are only used to describe specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly dictates otherwise.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present application. No.

Hereinafter, with reference to the attached drawings, a functional food for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention will be described in detail with preferred embodiments.

A functional food for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention contains hydrolyzed fermented horse placenta as an active ingredient.

In addition, the hydrolyzed fermented horse placenta may be included in 1 to 10% by weight of the total content of the functional food.

In the present invention, the term "food" refers to a natural product or processed product containing one or more nutrients, and preferably means that it has undergone a certain degree of processing to become ready to be eaten directly, and has the usual meaning. It is used to include all kinds of foods, health functional foods, beverages, food additives, and beverage additives.

For example, the above foods include various foods, beverages, gums, teas, vitamin complexes, functional foods, etc. In addition, foods in the present invention include special nutritional foods, processed meat products, fish products, tofu, jelly, and noodles (e.g. , ramen, noodles, etc.), health supplements, seasonings (e.g., soy sauce, soybean paste, red pepper paste, mixed paste, etc.), sauces, confectionery (e.g., snacks), dairy products (e.g., fermented milk, cheese, etc.), other processed foods, Kimchi, pickled foods (various kimchi, pickled vegetables, etc.), beverages (e.g., fruit and vegetable drinks, soy milk, fermented beverages, ice cream, etc.), natural seasonings (e.g., ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages, and Including, but not limited to, other health supplements. The health functional food, beverage, food additive, or beverage additive can be manufactured by conventional manufacturing methods.

Figure 1 is a flowchart schematically illustrating a method for producing hydrolyzed fermented horse placenta used in functional foods for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention.

Referring to Figure 1, the method for producing hydrolyzed fermented horse placenta according to an embodiment of the technical idea of the present invention includes the horse placenta preparation step (S100), soaking and washing step (S200), drying step (S300), and proteolysis. It includes an enzyme mixing step (S400), a proteolytic reaction step (S500), a purification step (S600), and a filtration step (S700).

1. Equine placenta preparation stage (S100)

The horse placenta preparation step (S100) is a step of preparing the placenta of a healthy horse.

In the horse placenta preparation step (S100), the horse placenta uses a normal placenta from a healthy horse that has been regularly examined by a veterinarian at the farm. For example, the horse placenta taken from the farm is washed in purified water at a temperature of 8 to 12 ° C. It may be a horse placenta that has been frozen in the freezer after removing the blood.

The horse placenta contains essential amino acids, nucleic acid components such as RNA and DNA, antioxidant enzyme SOD (Super Oxide Dismutase), hyaluronic acid, antioxidants, immune cofactors (cytocine), placental peptides, and insulin-like substances. Contains growth factors and cytokines such as insulin-like growth factors, EGFS (Epidermal Growth Factors), and unique senescent cell activating factors (SCAFS) to help relieve fatigue, It is known to be useful for strengthening immunity.

2. Soaking and washing step (S200)

The immersion and washing step (S200) is a step of immersing the prepared horse placenta in a fermented black pine needle shoot extract and then washing it with purified water.

Specifically, in the immersion and washing step (S200), the prepared horse placenta is immersed in black pine needle shoot fermentation extract at a temperature of 8 to 12 ° C for 15 to 25 hours and then separated, and the separated horse placenta is cooled at 5 to 10 ° C. This can be done by washing with purified water at any temperature.

In the immersion and washing step (S200), the horse placenta is immersed in black pine needle sprout fermented extract and then washed with purified water, thereby removing the blood remaining in the horse placenta and at the same time removing the unique off-flavor of the horse placenta. The fermented black pine needle shoot extract can be used as a fermented black pine needle shoot extract prepared by the following method.

In other words, the black pine needle shoot fermentation extract can be manufactured using pine needle shoots, which are leaves of the pine tree (Pinus densiflora Siebold et Zuccarini) and have a long history with our people in Northeast Asia, including Korea, China, and Japan. They are the leaves and shoots of one tree. Fresh pine needles contain a large amount of ascorbic acid, vitamins A, B, and K, bitter substances, flavonoids, anthocyanins, 7 to 12% resin, about 5% tannin, carbohydrates, essential oil, etc. It contains this.

In addition, the entire pine tree contains ingredients that expel body wastes such as alcohol and esters and promote metabolism, as well as phenolic compounds, quinine, terpentine, vitamins A, C, and chlorotyl as main ingredients, as well as glycoginin and apietic acid. Red pine leaves are rich in iron and contain 24 types of amino acids, and 19 types of amino acids composed of proteins were also identified.

The pine needles are a raw material and source of vitamin C, and the pine needles have a much darker green color, so they can be rich in carotene. In the case of pine needles, the pharmacological value can refer to the general effects of terpenes, phenolic compounds, tannins, etc. In addition, high blood pressure, stroke, diabetes, dementia and anti-aging, anti-cancer effect, liver and stomach, constipation and anemia, chronic alcoholism and hangover relief, nicotine removal, skin care and constitution improvement diet, stamina of men due to various stresses, excessive smoking and drinking. It is especially good for students and test takers who are studying to clear their heads when their head is heavy and their eyes are blurred.

The black pine needle shoot fermentation extract can be used as the black pine needle shoot fermentation extract prepared by the following method.

To prepare the black pine needle sprout fermented extract, first, pine needle sprouts can be prepared and then washed with purified water.

In the above step, dust or foreign substances attached to the pine needle shoots can be removed by preparing the pine needle shoots and then washing them in purified water at a temperature of 20 to 30°C.

Next, the washed pine needle sprouts can be immersed in rice water.

In the above step, the resin remaining in the pine needle shoots can be removed by immersing the washed pine needle shoots in rice water. Mixing 400 to 600 parts by weight of rice water with respect to 100 parts by weight of the total washed pine needle shoots. After immersion, the process can be carried out by maintaining the temperature at a temperature of 13 to 17° C. for 1 to 3 days.

Next, the pine needle sprouts immersed in the rice water can be separated and dried.

In this step, the pine needle shoots soaked in the rice water may be separated and then dried at a temperature of 20 to 30° C. for 20 to 40 hours.

Subsequently, the dried pine needle sprouts can be steamed to produce black pine needle sprouts.

In the above step, the black pine needle shoots can be produced by steaming the dried pine needles with steam for 5 to 7 days in a steamer at a temperature of 75 to 80 ° C.

Next, the steamed black pine needle shoots and the photosynthetic bacterial culture medium can be mixed and then fermented to produce a black pine needle sprout fermented liquid.

In the above step, 100 parts by weight of the steamed black pine needle shoots are mixed at a weight ratio of 20 to 30 parts by weight of the photosynthetic bacterial culture medium, and then fermented at a temperature of 43 to 47 ° C. for 5 to 7 days to prepare a black pine needle shoot fermented liquid. You can.

In addition, the photosynthetic bacterial culture medium contains 0.5 g of K ₂ HPO ₄ , 0.1 g of NaCl, 0.2 g of MgSO ₄ ·7H ₂ O, 3 g of Na-Acetate, 3 g of yeast extract and 1000 ml of H ₂ O, and inorganic elements Phosphorus CaCl ₂ ·2H ₂ O 0.5 g, FeCl·6H ₂ O 0.025 mg, CuSO ₄ ·5H ₂ O 0.00025 mg, H ₃ BO ₃ 0.005 mg, MnCl ₃ ·4H ₂ O 0.0003 mg and ZnSO ₄ ·7H ₂ O 0.005 After dissolving and mixing mg in order, sterilize and place in a culture bottle, and add at least one selected from the group consisting of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides , and Rhodospirillum rubrum . It can be manufactured by inoculating 100 ml of photosynthetic bacteria, filling it with nitrogen gas, sealing it to create an anaerobic state, and then culturing it for 3 days at 30°C under light conditions of 2,000 Lux.

Next, the black pine needle shoot fermentation liquid can be extracted to prepare the black pine needle shoot fermentation extract.

In the above step, the extraction of the black pine needle shoot fermentation liquid can be performed using various extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, and supercritical extraction. The composition of the extraction is a known technology, so it is convenient for explanation and present. For clarity of the technical idea of the invention, detailed description thereof will be omitted.

3. Drying step (S300)

The drying step (S300) is a step of drying the washed horse placenta.

In the drying step (S300), the moisture remaining in the horse placenta can be removed by drying the washed horse placenta. In the drying step (S300), the washed horse placenta is dried at a temperature of 20 to 30 ° C. for 10 to 30 minutes. This can be done by drying for some time.

4. Protease mixing step (S400)

The protease mixing step (S400) is a step of preparing a mixture by mixing protease and purified water with the dried horse placenta.

In the protease mixing step (S400), the mixture may be mixed in a weight ratio of 80 to 120 parts by weight of the dried horse placenta, 1 to 3 parts by weight of protease, and 150 to 200 parts by weight of purified water.

In the proteolytic enzyme mixing step (S400), the proteolytic enzyme may be bromelain, which is a vegetable proteolytic enzyme extracted from the fruits and roots of pineapple (Ananas comosus). Wow, it is a mixture of various compounds with other ingredients that have not yet been identified.

In addition, bromelain is a substance that decomposes muscle fibers well and is widely used in the treatment of anticancer, antiplatelet, respiratory inflammation, indigestion, rheumatoid arthritis, and mastitis. It is mainly used as an anti-inflammatory and anti-edema agent, and is known to be particularly effective in treating inflammation of soft tissue, traumatic events, and inflammation after tissue reaction after surgery. It is known that the treatment of inflammation occurs by bromelain improving the supply of nutrients to the inflamed area, such as decomposition of the fibrin layer in the inflamed area, anti-edema, eliminating pain and improving blood circulation, and strengthening immune function.

In other words, it is already widely known that bromelain, produced from pineapple through extraction and purification processes, is a type of vegetable proteolytic enzyme and has excellent anti-inflammatory effects. Looking at the pharmacological actions and characteristics of bromelain, (1) the absorbed enzyme decomposes and removes fibrin and denatured protein, which are inflammatory products, directly or indirectly through the fibrinolytic enzyme system, and (2) causes inflammation. It is effective by decomposing inflammatory polypeptides, bradykinin, etc., (3) improves local circulation inhibited by inflammatory products, and (4) is a natural plant enzyme with no side effects, so it is used in the pharmaceutical field as a treatment for arthritis and rheumatism. It is an enzyme that is used as a raw material for digestive agents and is also widely used in the food industry as a meat tenderizer and other additives.

Bromelain used as the proteolytic enzyme in the proteolytic enzyme mixing step (S400) may be a pineapple fermented extract containing bromelain component prepared by the following method.

That is, in order to prepare a pineapple fermented extract, the pineapple can first be prepared and then washed.

Next, the washed pineapple can be crushed.

The pineapple can be crushed using a grinder or blender to extract the active ingredients from the pineapple in a short period of time. For example, the pineapple can be crushed to a diameter of 1 to 3 cm.

Next, fermentation bacteria culture can be mixed with the crushed pineapple.

In this step, 100 to 20 parts by weight of the fermenting bacteria culture solution may be mixed with respect to 100 parts by weight of the total content of the pulverized pineapple. The fermentation bacteria culture solution may be a yeast culture solution.

The yeast culture medium may be a Moringa-extracted yeast ( Saccharomyces spp. ) culture medium. The Moringa-extracted yeast (Saccharomyces spp. ) culture medium is prepared by cultivating Moringa ( Moringa oleifera L. ) leaves at a temperature of 30 to 40°C. Dry Moringa leaves obtained by drying for 5 to 10 days are soaked in 40 times the volume of distilled water, extracted at 85 to 87°C and 1.5 Pa, concentrated by 50%, and then residues are removed to prepare a Moringa extract. Yeast bacteria ( Saccharomyces spp. ) were inoculated at a concentration of 5 × 1.0 ⁶ to 10 × 1.0 ⁶ CFU/mL, cultured at 38 to 42°C for 30 hours, and centrifuged at 10,000 to 15,000 rpm for 10 to 15 minutes to obtain the supernatant. A moringa-extracted yeast culture solution prepared by harvesting and sterilizing at a temperature of 120 to 140° C. for 30 to 50 minutes can be used, and yeast ( Saccharomyces spp. ) can be used as the strain.

Subsequently, the pineapple mixed with the fermentation bacteria culture can be fermented to produce a pineapple fermentation product.

In this step, pineapple fermentation broth can be prepared by fermenting pineapple mixed with the fermentation bacteria culture solution for 3 to 5 days at a temperature of 40 to 42 ° C.

Next, the pineapple fermentation liquid can be extracted to prepare a pineapple fermentation extract.

Extraction of the pineapple fermentation liquid can be done using known extraction methods such as solvent extraction, hot water extraction, or supercritical extraction. For example, the pineapple fermentation extract is (a) 70% (v/) in 1000-1200 g of the pineapple fermentation liquid. v) First extraction with 10 to 12 liters of ethanol aqueous solution at 85 to 90°C for 3 to 4 hours, followed by secondary extraction with 4 to 6 liters of 70% (v/v) ethanol aqueous solution at 85 to 90°C for 2 to 3 hours. Upon completion, preparing the extract until the volume reaches 1000 to 1200 ml in a vacuum rotary evaporator; (b) Add water to the extract and dilute it 2 to 3 times, centrifuge at 14,000 to 15,000 rpm for 20 to 30 minutes to completely remove polymer fiber and solids, and then wash the collected supernatant with 1.2 to 1.5 times chloroform twice. Extracting step; and (c) removing the chloroform layer and extracting the aqueous layer three times with 1.2 to 1.5 times the volume of ethyl acetate to prepare a pineapple fermentation extract.

5. Proteolysis reaction step (S500)

The proteolytic reaction step (S500) is a step of producing a horse placenta proteolyzate by subjecting a mixture of the horse placenta, proteolytic enzyme, and purified water to a proteolytic reaction at a constant temperature.

In the proteolytic reaction step (S500), the horse placenta can be hydrolyzed by reacting a mixture of the horse placenta, protease, and purified water at a temperature of 60 to 70° C. for 5 to 15 hours.

In the proteolytic reaction step (S500), if the proteolytic reaction is performed below the above-mentioned lower limit range, a problem may occur in which the reaction does not occur properly, and if it is performed beyond the above-mentioned upper limit range, the enzyme is deactivated. The problem is that it is difficult for the hydrolysis reaction to occur completely, and the problem of denaturation of useful components may occur.

6. Purification step (S600)

The purification step (S600) is a step of purifying the horse placental proteolyzate.

In the purification step (S600), by purifying the horse placental protein decomposition product, off-flavor or unpleasant odor remaining in the horse placental protein decomposition product can be removed, harmful substances can be adsorbed and removed, and a purification effect, etc. can be achieved. The purification step (S600) is mixed in a weight ratio of 10 to 20 parts by weight of a mixed powder in which activated carbon and cloisonne powder are mixed at a weight ratio of 3:1 with respect to 100 parts by weight of the total content of the horse placenta protein decomposition product, and then stirred at a temperature of 8 to 12° C. After being stored for up to 5 hours, the activated carbon and hempstone powder can be separated and removed.

At this time, the activated carbon is a carbon aggregate with developed micropores, and can change the internal surface area through the activation process. It has excellent absorbency and adsorption properties by adsorbing the surrounding liquid or gas through the internal carbon atom functional group.

The activated carbon has a hardness of 93% by mass or more, a packing density of 0.51 to 0.53g/ml, a specific surface area of 800 to 900m ² /g, a pore distribution of 4Å to 7Å in pore diameter, an iodine adsorption capacity of 1,100mg/g or more, and a pore volume of 0.51ml. /g, pH 7~7.8, phenol adsorption power of 17.5ml/g, M·B decolorization power of 153ml/g or more can be used.

In addition, the chiljeon can emit far-infrared rays and negative ions, and provide antibacterial, deodorizing and purifying effects. The chiljeon expands capillaries, prevents cancer, prevents dementia, and activates cells necessary for human activity. You can have

In addition, the seven gemstones are minerals with seven brilliant colors, including black, red, brown, red, gray, light gray, and green, and their main chemical components are SiO ₂ , Al ₂ O ₃ , Fe ₂ O ₃ , and FeO , CaO, MgO, Na ₂ O, TiO ₂ , P ₂ O ₅ and MnO are the main components, and are composed of quartz, feldspar, biotite, muscovite, garnet, apatite, zircon, calcite, chlorite, sericite and other minerals, energy The radiation form is a torsion wave in which the left and right waves radiate.

In particular, when looking at the compounds analyzed in the Chiljem, it contains more than 60% of SiO ₂ , so it has an excellent dehumidifying effect. It contains a large amount of activated alumina, etc., so it is rich in minerals and has a purifying effect by neutralizing alkali salts.

7. Filtration step (S700)

The filtration step (S700) is a step of producing a hydrolyzed fermented horse placenta product by filtering the purified horse placenta proteolyzate to remove foreign substances.

In the filtration step (S700), solids including activated carbon, cloisonne powder, etc. can be removed by filtering the purified horse placental proteolyzate using a known filter.

Since the configuration of filtering using a filter in the filtration step (S700) is a known technology, detailed description thereof will be omitted for convenience of explanation and clarity of the technical idea of the present invention.

Hereinafter, with reference to the attached drawings, a functional food for preventing and improving female menopausal symptoms using horse placenta according to an embodiment of the technical idea of the present invention will be described in more detail.

<Example>

First, after preparing the horse placenta, the prepared horse placenta was immersed in a fermented black pine needle shoot extract at a temperature of 10°C for 20 hours and then separated, and the separated horse placenta was washed with purified water at a temperature of 8°C.

Next, the washed horse placenta was dried at a temperature of 25°C for 20 hours, and then a mixture was prepared by mixing the dried horse placenta with protease and purified water.

At this time, the mixture was prepared by mixing 100 parts by weight of the dried horse placenta, 2 parts by weight of proteolytic enzyme, and 175 parts by weight of purified water, and the proteolytic enzyme was a pineapple fermentation product containing bromelain. The extract was used.

Next, the mixture was proteolytically reacted at a temperature of 65°C for 10 hours to prepare horse placenta proteolyzate. Based on 100 parts by weight of the total content of the horse placenta proteolyzate, activated carbon and cloisonne powder were added in a weight ratio of 3:1. The horse placenta proteolyzate was purified by mixing the mixed powder in a weight ratio of 15 parts by weight and storing it at a temperature of 10° C. for 3 hours, then separating and removing the activated carbon and cloisonne powder.

Subsequently, the purified horse placenta proteolyzate was filtered to remove foreign substances to prepare horse placenta hydrolyzed fermentation product.

<Experimental Example 1>

Blood estrogen concentration test

SD (Sprague-Dawley) 7-week-old female rats were purchased and acclimatized to the breeding farm and formula feed for 3 weeks. Only healthy animals were selected and used in the experiment. A female menopausal disease model rat was created by making a 1-2cm skin incision in the lower middle area of the abdomen of a selected 10-week-old female SD rat, then pulling and resecting the ovary and oviduct using forceps. .

Next, the hydrolyzed fermented horse placenta prepared according to the above example was orally administered to the model rat at a daily dose of 250 mg/kg for 4 weeks. Four weeks after administration, blood was collected from normal female SD rats, female menopausal disease model rats, and rats administered hydrolyzed fermented horse placenta, and the concentration of estradiol (a type of estrogen) in the collected blood serum was measured. , the results are shown in Figure 2.

Figure 2 is a graph showing the results of analyzing the effect of hydrolyzed fermented horse placenta on blood estrogen concentration using female menopausal disease model rats.

Referring to Figure 2, compared to normal rats, the serum concentration of estradiol was reduced in female menopausal disease model rats, and when horse placenta hydrolyzed fermentation product was administered to the female menopausal disease model rats, the serum concentration of estradiol was reduced. It was confirmed that the concentration increased again.

Therefore, it was confirmed that the hydrolyzed fermented horse placenta prepared according to the example was effective in improving the state of decreased female hormones that cause female menopausal diseases.

<Experimental Example 2>

Bone density increase rate experiment

Changes in bone density were analyzed in the female menopausal disease model rat prepared according to Experimental Example 1 and the model rat administered with the hydrolyzed fermented horse placenta prepared according to the Example at a dose of 250 mg/kg.

The femur was separated from each rat, the surrounding muscles were removed, and it was fixed in 10% formalin. Bone density was measured through μCT analysis, and bone volume density (BV/TV (%)) was calculated by calculating the cancellous bone volume (BV) relative to the total bone area (TV) at the distal femoral epiphysis. The relative ratio (bone density increase ratio) was calculated to analyze the effect of improving osteoporosis.

At this time, normal rats were used as the control group, and the results are shown in Figure 3.

Figure 3 is a graph showing the results of analyzing the effect of hydrolyzed fermented horse placenta on osteoporosis caused by female menopausal disease using female menopausal disease model rats.

Referring to Figure 3, in the case of female menopausal disease model rats (menopausal induced group), the rate of increase in bone density was decreased compared to normal rats, but in rats administered hydrolyzed fermented horse placenta, it was higher than that in female menopausal disease model rats. , it was confirmed that the bone density increase rate was improved.

<Experimental Example 3>

Cytotoxicity evaluation of hydrolyzed fermented horse placenta

In order to confirm the safety of the hydrolyzed fermented horse placenta prepared according to the above example, the cytotoxicity of the extract on osteoblasts was evaluated. Specifically, the osteoblast cell line C3H10T1/2 was distributed in a 96-well plate, counted at 2 It was cultured for some time. After completion of incubation, the water-insoluble formazan formed by the addition of the MTT solution was dissolved with a dimethyl sulfoxide solution, and the optical density (OD. value) of the dissolved formazan was measured at 595 nm. Relative cell activity (%) was evaluated by measuring (Figure 4). At this time, osteoblasts treated with DMSO (dimethyl sulfoxide) were used as the control group.

Figure 4 is a graph showing the toxicity of the hydrolyzed fermented horse placenta prepared according to the example to osteoblasts.

Referring to Figure 4, it was confirmed that the hydrolyzed fermented horse placenta prepared according to the example did not exhibit cytotoxicity even at concentrations of 100, 250, and 500 μg/mL, and through this, the horse placenta hydrolyzed product according to the present invention It was found that the decomposed and fermented product is a safe substance that is not harmful to the human body.

Although a preferred embodiment of the present invention has been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical idea or essential features. You will be able to. Therefore, the embodiment described above should be understood in all respects as illustrative and not restrictive.

Claims (7)

Contains hydrolyzed fermented horse placenta as an active ingredient,
The hydrolyzed fermented horse placenta contains 1 to 10% by weight of the total functional food content,
The hydrolyzed fermented horse placenta,
Equine placenta preparation step (S100) to prepare the horse placenta;
A immersion and washing step (S200) in which the prepared horse placenta is immersed in black pine needle sprout fermentation extract and then washed with purified water;
A drying step (S300) of drying the washed horse placenta;
A protease mixing step (S400) of mixing the dried horse placenta with protease and purified water to prepare a mixture;
Proteolysis reaction step (S500) of producing horse placenta proteolyzate by proteolytic reaction of the mixture of horse placenta, proteolytic enzyme and purified water;
A purification step (S600) of purifying the horse placental proteolyzate; and
Prevention and improvement of female menopausal symptoms using horse placenta, characterized in that it is manufactured through a process including a filtration step (S700) of producing horse placenta hydrolyzed fermentation product by filtering the purified horse placenta protein hydrolyzate to remove foreign substances. For functional food.
delete delete According to clause 1,
In the immersion and washing step (S200), the prepared horse placenta is immersed in black pine needle shoot fermentation extract at a temperature of 8 to 12 ℃ for 15 to 25 hours and then separated, and the separated horse placenta is soaked in purified water at a temperature of 5 to 10 ℃. A functional food for preventing and improving female menopausal symptoms using horse placenta, which is carried out by washing with .
According to clause 4,
In the protease mixing step (S400), the mixture is a horse placenta, characterized in that 80 to 120 parts by weight of the dried horse placenta, 1 to 3 parts by weight of protease, and 150 to 200 parts by weight of purified water are mixed in a weight ratio. Functional food for preventing and improving female menopausal symptoms.
According to clause 5,
In the proteolytic enzyme mixing step (S400), bromelain is used as the proteolytic enzyme, and bromelain contained in pineapple fermented extract is used. Functional food for preventing and improving female menopausal symptoms using placenta.
According to clause 6,
The purification step (S600) is performed by mixing 10 to 20 parts by weight of a mixed powder in which activated carbon and cloisonnite powder are mixed at a weight ratio of 3:1 with respect to 100 parts by weight of the total content of the horse placenta proteolyzate, and then 8 to 20 parts by weight. A functional food for preventing and improving female menopausal symptoms using horse placenta, which is stored at a temperature of 12°C for 1 to 5 hours and then separated and removed from the activated carbon and cloisonne powder.