KR102178926B1 - A composition for reinforcing immune function and anti-fatigue comprising fermented placenta and its use - Google Patents

Abstract

The present invention is (a) any one selected from the group consisting of fucoidan, coenzyme Q10, and snail extract; And (b) it relates to a pharmaceutical composition for enhancing immunity or promoting fatigue recovery, including a fermented placenta, a health functional food composition, or a feed composition.

Description

A composition for reinforcing immune function and anti-fatigue comprising fermented placenta and its use, comprising a fermented placenta composition as an active ingredient

The present invention is (a) any one selected from the group consisting of fucoidan, coenzyme Q10, and snail extract; And (b) it relates to a pharmaceutical composition for enhancing immunity or promoting fatigue recovery, including a fermented placenta, a health functional food composition, or a feed composition.

Since the incidence of infections and diseases mainly occurs in a state of reduced immune function, various studies have been conducted to enhance these immune responses through immune enhancing substances when the function of the body's immune system is reduced.

On the other hand, macrophages are known to be involved in host defense and homeostasis by engaging in various host responses such as adaptive immunity as well as intrinsic immunity, and IL-1β (interleukin-1β), IL-6( Interleukin-6) and TNF-α (tumor necrosis factor-α) by producing cytokines (cytokines) are known to play an important role in the body defense in the early stages of infection (Journal of Pharmacy 2013; 57(6): 394). ~399).

Chang-Bae Jeon et al. (Journal of the Korean Pharmacopoeia 2013; 57(6): 394~399) describe the effect of enhancing macrophage immunity of the extract of Polygonum aviculare L., and Sunmi Heo et al. (Kor. J. Pharmacogn. 2011; 42( 3): 246~252) describe the immune enhancing effect of lectin (KML-C) isolated from mistletoe from Korean mistletoe. However, it has not been reported that the complex composition of fermented placenta, coenzyme Q10, fucoidan, and snail extract is effective in enhancing immunity.

On the other hand, with regard to anti-fatigue agents, Korean Patent Application Publication No. 2012-0033443 describes the anti-fatigue effect of rhubarb extract.

The causes of fatigue are classified into a lack or inability to use energy sources stored in the body, accumulation of fatigue substances due to metabolism, and loss of homeostasis in the body, but are known to be made mostly by synergistic action of many factors. In order to recover from such fatigue, supply of sufficient energy sources, rest, suppression and removal of fatigue substances in the body are required, but in fact, sufficient nutrition intake and rest are not achieved in a busy modern society, so the discovery of functional materials for fatigue recovery The necessity of this is emerging.

Under this background, the present inventors have made intensive research efforts to develop natural materials that are effective in enhancing immunity or recovering from fatigue, and as a result, a complex composition of fermented placenta, coenzyme Q10, fucoidan, and snail extract has an effect on enhancing immunity and recovering from fatigue. And completed the present invention.

The main object of the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) to provide a pharmaceutical composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

Another object of the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) to provide a health functional food composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

Another object of the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) to provide a feed composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

As an aspect for achieving the above object, the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) it provides a pharmaceutical composition for enhancing immunity or promoting fatigue recovery comprising a fermented placenta.

According to an embodiment of the present invention, the composition improved the proliferation ability of macrophages RAW 264.7 in a concentration-dependent manner (Experimental Example 1, Fig. 1), and the amount of nitrogen monoxide (NO) produced by activated macrophages Was increased (Experimental Example 2, Fig. 2), and was found to increase the production of tumor necrosis factor alpha (TNF-α) by the activity of macrophages (Experimental Example 3, Fig. 3). That is, the composition promotes immune cell proliferation, nitro oxide (NO) production, and tumor necrosis factor alpha (TNF-α) production, and thereby may exhibit an immune enhancing effect.

According to another embodiment of the present invention, as a result of administering the composition to a subject who usually feels fatigue, it was found that the subject's fatigue recovery effect was excellent (Experimental Example 4, Table 3).

Hereinafter, the pharmaceutical composition in the present invention will be described in detail.

The placenta may mean a structure that mediates material exchange necessary for the survival and growth of the fetus between the fetus and the mother. In the present invention, fermented placenta may be fermented by purchasing a commercially sold placenta, or commercially sold fermented placenta may be used, but is not limited thereto. The fermentation method may be used without limitation as long as it is a method used for fermentation of the placenta composition in the art. The placenta may be, for example, a mammalian placenta.

The coenzyme Q10 is one of the coenzymes that work in the body and is a vitamin-like action factor called ubiquinone and vitamin Q. In the present invention, coenzyme Q10 may be purchased commercially or derived from nature.

The fucoidan is a sulfated polysaccharide having a sticky viscous structure, and is a substance in which a basic sugar called fucose and a sulfuric acid group are combined. In the present invention, the fucoidan may be purchased commercially or purified from natural cultivation or harvesting. For example, it may be isolated from brown algae such as seaweed, kelp, seaweed, sugar kelp (Laminaria saccharina), L. digitata, Fucus distichus, and Okinawa mozuku , Is not limited thereto.

Snails are gastropods, or snails belonging to the family Fruticiola sieboldiana, and about 20,000 species are known in the world. The major nutrients of snails are known as protein (54.29%), lipids (4.18%), carbohydrates (30.45%), and ash (4.07%), and are particularly rich in protein and calcium. The component that makes up the sticky mucus secreted from the snail is called mucin, and the main component that makes up mucin is chondroitin sulfate. Skin moisturizing, skin elasticity, whitening, and skin wrinkle improvement effects of snail extract are known, but no immunity enhancement or fatigue recovery effect thereof is known.

In the present invention, the snail extract may be purchased commercially sold, or may be used by extracting cultivated or harvested in nature. For example, the extract may mean a resultant product such as a liquid component obtained by immersing a target substance in various solvents and then extracting at room temperature or warming state for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. have. In addition, in addition to the resulting product, it can be comprehensively interpreted as including all of the diluted solution of the resultant, the concentrate thereof, the preparation thereof, and the purified product. The method for obtaining the extract is not particularly limited as long as an extract having an immune enhancing or fatigue recovery effect can be obtained, but, for example, the snail is immersed in a solvent and extracted at room temperature of 10 to 25°C. Cold sedimentation extraction, heating extraction at 40 to 100°C for extraction, ultrasonic extraction by applying ultrasonic waves, and reflux extraction using a reflux cooler may be used.

For example, the composition may include fermented placenta, coenzyme Q10, fucoidan, and snail extract.

In the present invention, immunity enhancement refers to enhancing the biological defense ability against an antigen, and specifically, may mean increasing cellular and humoral immunity against the antigen. The mechanism of immunity enhancement is not limited, but may include, for example, promoting the activity of antigen-providing cells such as macrophages or promoting specific activity for lymphocytes.

According to an embodiment of the present invention, the complex composition comprising fermented placenta, coenzyme Q10, fucoidan, and snail extract all enhanced the proliferation ability of macrophage RAW 264.7 in a concentration-dependent manner (FIG. 1), and activated macrophages It was found to increase the production amount of NO known to be secreted (FIG. 2) and to increase the production of TNF-α by the activity of macrophages (FIG. 3 ).

That is, the composition promotes immune cell proliferation, NO production, and TNF-α production, and thereby may exhibit an immune enhancing effect.

In the present invention, fatigue recovery refers to a state in which accumulated fatigue is eliminated or fatigue symptoms are removed.

According to an embodiment of the present invention, as a result of administering a composite composition containing all of the fermented placenta, coenzyme Q10, fucoidan, and snail extract to an individual who feels tired normally, it was found that the fatigue recovery effect of the individual was excellent (Table 3). .

In the composition, the weight ratio of fermented placenta, coenzyme Q10, fucoidan, and snail extract may be 100: 0.1 to 5: 0.1 to 5: 0.1 to 5. For example, the weight ratio may be 100: 0.5 to 3: 0.5 to 3: 0.5 to 3. When the concentration of coenzyme Q10, fucoidan, and snail extract is lower than the above ratio, the physiological activity is hardly exhibited, and the functional level is insignificant compared to the fermented placenta alone material. In addition, if the ratio is higher than the above ratio, it may adversely affect the quality of the composition due to off-flavor, moisture absorption, or discoloration of the raw material itself.

The composition is a solution, gel, emulsion, suspension, micro-emulsion, microcapsule, liposome, cream, lotion, ointment, aerosol ( aerosol), spray, paste, and patch (patch) may be any one formulation selected from the group.

The pharmaceutical composition of the present invention may be used as a single formulation, and may be prepared and used as a combined formulation, including additional drugs known to have an approved immunity enhancing or fatigue recovery effect, and formulated using a pharmaceutically acceptable carrier or excipient By doing so, it can be manufactured in a unit dosage form or it can be manufactured by placing it in a multi-dose container.

As used herein, the term "pharmaceutically acceptable carrier" may mean a carrier or diluent that does not stimulate an organism and does not inhibit the biological activity and properties of the compound to be injected. The kind of the carrier that can be used in the present invention is not particularly limited, and any carrier commonly used in the art and pharmaceutically acceptable can be used. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in combination of two or more. The carrier may include a non-naturally occurring carrier.

In addition, if necessary, other conventional additives such as antioxidants, buffers and/or bacteriostatic agents can be added and used, and diluents, dispersants, surfactants, binders, lubricants, etc. can be additionally added to provide main solutions such as aqueous solutions, suspensions, and emulsions. It can be formulated and used as a dosage form, pill, capsule, granule, or tablet.

In addition, the pharmaceutical composition of the present invention may contain a pharmaceutically effective amount of fermented placenta, coenzyme Q10, fucoidan, and snail extract. In the present invention, the term "pharmaceutically effective amount" means an amount sufficient to induce an immune enhancing or fatigue recovery effect at a reasonable benefit/risk ratio applicable to medical treatment, and generally in an amount of 0.001 to 1000 mg/kg , Preferably 0.05 to 200 mg/kg, more preferably 0.1 to 100 mg/kg may be administered once to several times a day. However, for the purposes of the present invention, a specific therapeutically effective amount for a specific patient is a specific composition, including the type and degree of reaction to be achieved, whether other agents are used in some cases, the patient's age, weight, general health status, It is preferable to apply differently according to various factors including sex and diet, administration time, administration route and secretion rate of the composition, treatment period, drugs used with or concurrently with the specific composition, and similar factors well known in the medical field.

In another aspect, the present invention provides a method for enhancing immunity or recovering from fatigue comprising administering fermented placenta, coenzyme Q10, fucoidan, and snail extract to an individual.

As used herein, the term "individual" may mean all animals, including humans, in need of or possibly needing to enhance immunity or recover from fatigue. The animals may be mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, cats, etc. in need of treatment for symptoms similar to humans, but are not limited thereto.

The prevention or treatment method of the present invention may specifically include administering the composition to an individual in a pharmaceutically effective amount.

The term "administration" used in the present invention means introducing the pharmaceutical composition of the present invention to a patient by any suitable method, and the route of administration of the composition of the present invention is various oral or parenteral as long as it can reach the target tissue. It can be administered via the route.

The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with a conventional therapeutic agent. And can be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without causing side effects in consideration of all of the above factors, and can be easily determined by a person skilled in the art.

The method of administration of the pharmaceutical composition according to the present invention is not particularly limited, and may be followed by a method commonly used in the art. As a non-limiting example of the administration mode, the composition may be administered orally or parenterally. The pharmaceutical composition according to the present invention may be prepared in various formulations according to the intended administration method.

The frequency of administration of the composition of the present invention is not particularly limited thereto, but may be administered once a day or several times by dividing the dose.

In another aspect, the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) it provides a health functional food composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

For example, the composition may include fermented placenta, coenzyme Q10, fucoidan, and snail extract.

Since the fermented placenta, coenzyme Q10, fucoidan, and snail extract have been used as natural substances for a long time and have proven their stability, they can be prepared and consumed in the form of foods capable of enhancing immunity or recovering from fatigue while having common sense.

Functional food is the same term as food for special health use (FoSHU), and refers to foods with high medical and medical effects that have been processed to efficiently display bioregulatory functions in addition to nutritional supply. . The food may be prepared in any one formulation selected from the group including powders, granules, tablets, capsules, liquids, and pills in order to obtain a useful effect in enhancing immunity or recovering from fatigue, but is commonly used in the art. Any formulation can be used without limitation.

The food composition of the present invention may further include a food pharmaceutically acceptable carrier.

There is no particular limitation on the type of food to which the composition of the present invention can be added, and for example, there are various beverages, gums, teas, vitamin complexes, and health supplement foods. Other ingredients that do not interfere with the effect of enhancing immunity or recovering from fatigue may be added to the food composition, and the kind is not particularly limited. For example, it may contain various herbal extracts, food additives, or natural carbohydrates as an additional component, as in ordinary foods.

The food supplementary additives are added to prepare health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art. For example, various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters , Stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. are included, but the types are not limited by the above examples.

At this time, the content of the extract contained in the food is not particularly limited thereto, but may be included in 0.01 to 100% by weight, more preferably 1 to 80% by weight based on the total weight of the food composition.

When the food is a beverage, it may be included in a ratio of 1 to 30 g, preferably 3 to 20 g, based on 100 ml. In addition, the composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), and copper (Cu) may be included. In addition, amino acids such as lysine, tryptophan, cysteine, and valine may be included. In addition, preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleached and highly bleached, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxytoleuene ( BHT), etc.), colorants (tar color, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasoning (MSG sodium glutamate, etc.), sweeteners (dulsin, cyclamate, saccharin, sodium, etc.) , Flavorings (vanillin, lactones, etc.), expanding agents (alum, D-potassium hydrogen stannate, etc.), reinforcing agents, emulsifying agents, thickening agents (thickening agents), coating agents, gum base agents, foam inhibitors, solvents, and food additives such as improving agents. ) Can be added. The additive is selected according to the type of food and used in an appropriate amount.

The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and ingredients commonly added in the art. In addition, unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time by using food as a raw material, and it can be excellent in portability.

In the composition, the weight ratio of fermented placenta, coenzyme Q10, fucoidan, and snail extract may be 100: 0.1 to 5: 0.1 to 5: 0.1 to 5. For example, the weight ratio may be 100: 0.5 to 3: 0.5 to 3: 0.5 to 3. When the concentration of coenzyme Q10, fucoidan, and snail extract is lower than the above ratio, the physiological activity is hardly exhibited, and the functional level is insignificant compared to the fermented placenta alone material. In addition, if the ratio is higher than the above ratio, it may adversely affect the quality of the composition due to off-flavor, moisture absorption, or discoloration of the raw material itself.

In another aspect, the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) it provides a feed composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

For example, the composition may include fermented placenta, coenzyme Q10, fucoidan, and snail extract.

The feed composition may contain a feed additive. The feed additive of the present invention corresponds to an auxiliary feed in the feed management law.

In the present invention, the term "feed" may mean any natural or artificial diet, one meal meal, etc., or a component of the one meal meal, for animals to eat, ingest, and digest.

The kind of feed is not particularly limited, and feed commonly used in the art may be used. Non-limiting examples of the feed include vegetable feeds such as grains, root fruits, food processing by-products, algae, fiber, pharmaceutical by-products, oils and fats, starches, meals or grain by-products; Animal feeds such as proteins, inorganic logistics, oils and fats, minerals, oils and fats, single-cell proteins, zooplanktons or foods. These may be used alone or in combination of two or more.

In the composition, the weight ratio of fermented placenta, coenzyme Q10, fucoidan, and snail extract may be 100: 0.1 to 5: 0.1 to 5: 0.1 to 5. For example, the weight ratio may be 100: 0.5 to 3: 0.5 to 3: 0.5 to 3. When the concentration of coenzyme Q10, fucoidan, and snail extract is lower than the above ratio, the physiological activity is hardly exhibited, and the functional level is insignificant compared to the fermented placenta alone material. In addition, if the ratio is higher than the above ratio, it may adversely affect the quality of the composition due to off-flavor, moisture absorption, or discoloration of the raw material itself.

In another aspect, the present invention is any one selected from the group consisting of (a) fucoidan, coenzyme Q10, and snail extract; And (b) it provides a quasi-drug composition for enhancing immunity or promoting fatigue recovery, comprising a fermented placenta.

For example, the composition may include fermented placenta, coenzyme Q10, fucoidan, and snail extract.

In the present invention, the term "quasi-drug" refers to fibers, rubber products, or the like, which are used for the purpose of treating, alleviating, treating, or preventing diseases of humans or animals, weakly acting on the human body or not directly acting on the human body, and Or non-machine and similar, as an article corresponding to one of the preparations used for sterilization, insecticide and similar purposes for the prevention of infectious types, for the purpose of diagnosing, treating, alleviating, treating or preventing diseases of humans or animals. Among the items used, it may mean items other than those that are not instruments, machines, or devices, and those that are not used for the purpose of pharmacologically affecting the structure and function of humans or animals. In addition, the quasi-drug may include skin external preparations and personal hygiene products. Preferably, it may be a disinfectant cleaner, shower foam, gagrin, wet tissue, detergent soap, hand wash, or ointment, but is not limited thereto.

When the composition according to the present invention is used as a quasi-drug additive, the composition may be added as it is or may be used together with other quasi-drug or quasi-drug components, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use.

In the composition, the weight ratio of fermented placenta, coenzyme Q10, fucoidan, and snail extract may be 100: 0.1 to 5: 0.1 to 5: 0.1 to 5. For example, the weight ratio may be 100: 0.5 to 3: 0.5 to 3: 0.5 to 3. When the concentration of coenzyme Q10, fucoidan, and snail extract is lower than the above ratio, the physiological activity is hardly exhibited, and the functional level is insignificant compared to the fermented placenta alone material. In addition, if the ratio is higher than the above ratio, it may adversely affect the quality of the composition due to off-flavor, moisture absorption, or discoloration of the raw material itself.

The composition according to the present invention promotes immune cell proliferation, production of nitric oxide, and production of tumor necrosis factor alpha, and through this, can exhibit an immune enhancing effect.

In addition, the composition according to the present invention has the effect of inducing fatigue recovery in an individual who usually feels fatigue.

1 shows the effect of enhancing immunity by increasing the proliferation of macrophages according to Examples and Comparative Examples of the present invention.
Figure 2 shows the immunity enhancing effect by increasing the production of nitrogen monoxide (NO) according to the Examples and Comparative Examples of the present invention.
3 shows the effect of enhancing immunity by increasing the production of tumor necrosis factor alpha (TNF-α) according to Examples and Comparative Examples of the present invention.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Manufacturing example 1. Preparation of composite composition ( Example 1 to 8, Comparative example 1 to 3)

Non-fermented general pig placenta and fermented pig placenta were purchased and used from Horus, Japan, and a complex composition was prepared in the following ratio using a commercial product for fucoidan powder, coenzyme Q10, and snail extract. .

Cell culture solution treatment concentration (ppm) LPS (0.1ng/ml) treatment Normal
Money placenta Fermentation
Money placenta Fucoidan
powder Coenzyme
Q10 snail
extract Comparative Example 1 - - - - - x Comparative Example 2 - - - - - O Comparative Example 3 130 - - - - O Example 1 - 130 - - - O Example 2 - - One O Example 3 - - - One O Example 4 - - - - 1.5 O Example 5 - 130 1.5 - - O Example 6 - 130 1.5 One - O Example 7 - 13 . 0.1 0.1 O Example 8 - 130 One One 1.5 O

Preparation Example 2. Preparation of tablets

Using the fermented placenta, coenzyme Q10, fucoidan, and snail extract in the ratio of Table 2 and the remaining amount of excipients, 300 mg tablets were prepared.

Product composition (%) Normal
Money placenta Fermentation
Money placenta Fucoidan
powder Coenzyme
Q10 snail
extract Excipient Manufacturing Example 1 - - - - - 100 Manufacturing Example 2 13 0.15 0.1 0.1 Balance Manufacturing Example 3 13 0.15 0.1 0.1 Balance

Experimental Example 1. Effect of promoting proliferation of macrophages by the composite composition

Because macrophages have phagocytosis, they can eat antigens and thus represent important functions in natural immunity. They can destroy microorganisms, antigens, dead tissue, and red blood cells through phagocytosis.

Macrophages are representative secretory cells that secrete various substances out of cells, and secrete about 50 or more active substances. These macrophage secretions play an important role in the regulation of the inflammatory response. For example, various proteolytic enzymes, lipid metabolites, and cytokines are known to play an important role in the progress and maintenance of inflammatory reactions. Macrophages also secrete various plasma proteins that can prevent the destruction of tissues due to inflammatory reactions, and also secrete blood coagulation factors and complement proteins. In addition, they secrete angiogenesis factors, and regulate the action of other immune cells. It also secretes cytokines. Therefore, the proliferation of macrophages has important implications for the immune system. Therefore, by measuring the proliferation ability of macrophages by the test substance, the immunity enhancing effect of the test substance can be evaluated.

First, mouse macrophages RAW 264.7 (Mouse macrophage cell line, ATCC The Global Bioresource Center, USA) were purchased, and 100 µl each was dispensed into a 96-well microplate at a cell concentration of 2.5 x 10 ⁶ cells/ml. The medium was DMEM (GibcoBRL, Grand Island, NY, USA) supplemented with 10% FBS (GibcoBRL, Grand Island, NY, USA) and 1% penicillin/streptomycin (Thermo Scientific Hyclone, Waltham, MA, USA). , 5% CO2, and cultured in an environment at 37°C. Inflammation was induced by treatment with 0.1 ng/ml of LPS (Lipopolysaccharide) (Sigma, St. Louis, MO, US) in the cell culture medium dispensed with the RAW 264.7 cells (Comparative Example 1 was not treated with LPS).

Thereafter, the sample prepared according to Preparation Example 1 was treated in a culture solution and cultured for 3 days. To measure the degree of growth, CCK-8 (Cell Counting Kit-8, Dojindo Co. Ltd., Kumamoto, Japan) was used according to the manufacturer's instructions.

As a result, as shown in Fig. 1, when LPS is treated, the reduced macrophage proliferation ability is greatly increased when the complex composition (Example 8) containing all fermented placenta, coenzyme Q10, fucoidan, and snail extract is treated. Appeared to be.

These results suggest that the composite composition can exhibit an immune enhancing effect by promoting macrophage proliferation ability.

Experimental example 2. Effect of promoting nitrogen monoxide production by the composite composition

Nitric Oxide (NO) is an important signal transducer that acts as a defense against foreign substances in the immune system. L-arginine is converted into L by the action of nitric oxide synthase (NOS). -Citrulline (L-citruline) is produced in the process of being transformed and is known to act as a protective substance in the immune system. In the immune system, it is known that when immune-effect cells are induced to produce a large amount of nitrogen monoxide under the influence of a cell-active substance, which is an immunotransmitter, a large amount of nitrogen monoxide is released to the immune inflammatory reaction site, thereby exhibiting an immune response. In particular, when bacteria are decomposed by bactericidal action in macrophages, macrophages secrete nitrogen monoxide to kill bacteria. Accordingly, by measuring the amount of nitrogen monoxide known to be secreted by activated macrophages, it is possible to confirm the activity of enhancing immune function by the test substance.

First, inflammation was induced in macrophage RAW 264.7 in the same manner as in Experimental Example 1, and the sample prepared according to Preparation Example 1 was treated in the culture solution. Thereafter, the cells were cultured for 24 hours, centrifuged at 900 rpm and 4° C. for 5 minutes, and then the cell culture solution was recovered to confirm the nitrogen monoxide production ability produced in the culture solution. The amount of nitrogen monoxide in the cell culture was measured using Griess reagent (Promega Co., Madison, WI, USA) according to the manufacturer's instructions.

As a result, as shown in Figure 2, when treated with LPS, the reduced NO production ability was significantly increased when the complex composition (Example 8) containing all fermented placenta, coenzyme Q10, fucoidan, and snail extract was treated. appear.

In particular, compared to the treatment of fermented placenta (Example 1), fucoidan (Example 2), coenzyme Q10 (Example 3), and snail extract (Example 4) alone, the NO production ability was significantly increased when the composite composition was treated. Appeared to be.

These results suggest that the composite composition can exhibit an immunity enhancing effect by promoting the NO production ability.

Experimental example 3. Tumor necrosis factor alpha ( TNF -α) production promotion effect

TNF-α is a cytokine that is a member of the acute-phase protein, especially among the inflammatory reactions. TNF-α is mainly secreted by activated macrophages, and is also secreted by various cells such as helper T cells, natural killer cells, and neurons. TNF- is an inflammatory cytokine secreted from activated macrophages or splenocytes, and enhances the elimination activity of pathogens and tumor cells invading the human body. TNF-α is a cytokine that interacts with lymphocytes to regulate the activity and growth of lymphocytes. In particular, it is a cytokine that induces Th-1 cell responses. It enhances immunity in diseases such as cerebral infarction and cancer. In addition, TNF-α also exhibits direct anti-cancer action by inducing cell lysis of cancer cells. Therefore, by observing the effect of the test substance on the production of TNF-α, the effect of enhancing immunity by the test substance can be evaluated.

First, inflammation was induced in macrophage RAW 264.7 in the same manner as in Experimental Example 1, and the sample prepared according to Preparation Example 1 was treated in the culture solution. Thereafter, the cells were cultured for 24 hours, and after centrifugation at 900 rpm for 5 minutes at 4° C., the cell culture solution was recovered to confirm the production ability of TNF-α produced in the culture solution. TNF-α was measured according to the manufacturer's instructions using an ELISA kit (BD Biosciences, Co., Ltd., San Diego, CA, USA).

As a result, as shown in Fig. 3, when LPS is treated, the reduced TNF-α production ability is very much when the complex composition (Example 8) containing all fermented placenta, coenzyme Q10, fucoidan, and snail extract is treated. Appeared to increase.

In particular, compared to the fermented placenta (Example 1), fucoidan (Example 2), coenzyme Q10 (Example 3), and the snail extract (Example 4) treated alone, the TNF-α production ability when the composite composition was treated Appeared to increase significantly.

These results suggest that the composite composition can exhibit an immune enhancing effect by promoting the ability to produce TNF-α.

Experimental example 4. The effect of promoting fatigue recovery by the composite composition

Ten adults aged 20-50 years old who usually feel tired were recruited for each group, and the tablets of Preparation Example 2 were taken 3 tablets a day for 3 months. In order to evaluate the subjective satisfaction of the five items on the improvement of fatigue before/after ingestion, it was evaluated on a 5-point scale, and the results are shown in Table 3.

Item Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Before intake After intake change Before intake After intake change Before intake After intake change Tired all day
Sleepy 4.2 3.8 ▼0.4 4.8 3.6 ▼1.2 4.4 3.0 ▼1.4 Even if I sleep, it's refreshing
Without heavy body. 3.2 3.4 △0.2 3.0 2.0 ▼1.0 3.6 2.2 ▼1.4 Little body activity
Heavy and tired 4.2 4.0 ▼0.2 4.6 3.6 ▼1.0 4.0 3.0 ▼1.0 Sometimes I am tired but I can't sleep. 3.2 3.2 - 3.0 2.4 ▼0.6 3.4 2.2 ▼1.2 Snoring badly when sleeping
Gonda. 1.6 1.8 △0.2 1.0 1.0 - 1.2 1.0 ▼0.2

As shown in Table 3, the group ingesting tablets containing no composition and containing only excipients (Preparation Example 1) showed little difference before/after ingestion in all items of the subjective satisfaction questionnaire.

On the other hand, in the group ingesting tablets containing fermented placenta, fucoidan, coenzyme Q10, and snail extract, subjective effects were observed in all questionnaires. Appeared large.

These results suggest that the composite composition containing all of fermented placenta, coenzyme Q10, fucoidan, and snail extract has a fatigue recovery effect.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof, are included in the scope of the present invention.

Claims (6)

Fucoidan (fucoidan), coenzyme Q10 (coenzyme Q10), containing a snail extract, and fermented placenta, a pharmaceutical composition for promoting fatigue recovery.
The method of claim 1,
The weight ratio of the fermented placenta, coenzyme Q10, fucoidan, and snail extract is 100: 0.1 to 5: 0.1 to 5: 0.1 to 5, the pharmaceutical composition.
The method of claim 1,
The composition is to promote immune cell proliferation, nitrogen monoxide (NO) production, and tumor necrosis factor alpha (TNF-α) production.
Fucoidan (fucoidan), coenzyme Q10 (coenzyme Q10), containing a snail extract, and fermented placenta, health functional food composition for promoting fatigue recovery.
The method of claim 4,
The weight ratio of the fermented placenta, coenzyme Q10, fucoidan, and snail extract is 100: 0.1 to 5: 0.1 to 5: 0.1 to 5, health functional food composition.
Fucoidan (fucoidan), coenzyme Q10 (coenzyme Q10), containing a snail extract, and fermented placenta, a feed composition for promoting fatigue recovery.

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