KR101988959B1 - Stress relieving or anti-fatigue composition containing enzymatic treatment white grub - Google Patents

Abstract

The present invention relates to a composition containing white grub enzyme-treated materials, a black ginseng extract, an Acanthopanax sessiliflorum extract, an Angelica gigas extract, a Polygala tenuifolia extract, an Acorus gramineus extract, a Poria cocos extract, and a mushroom extract. A health functional food containing the composition has an excellent stress relieving effect, anti-fatigue effect or endurance enhancement effect, thereby being able to be used as a luxury food or an additive for restoring physical strength of a child, a student in an adolescence, an examinee, an elderly person, a patient in a recovery period and the like.

Description

TECHNICAL FIELD [0001] The present invention relates to a stress relieving or anti-fatigue composition containing enzymatic treatment of slugs,

The present invention relates to a stress relieving or anti-fatigue composition containing a slug enzyme-treated product.

Fatigue can sometimes be defined as "a perceived reduced ability to physical and mental activity due to an imbalance in the availability, utilization, and recovery of the resources needed to perform the activity," and specifically fatigue is primarily characterized by physical fatigue And the stress is the state of mental fatigue that causes confusion of antacids (KFDA's functional evaluation system for fatigue recovery, research report). Fatigue is classified as central nervous system fatigue, fatigue of nerve-muscle joints, and peripheral fatigue of fatigue. Fatigue is a comprehensive physiological process that involves a variety of physiological and biochemical factors, and is a mental physiological phenomenon that necessarily occurs when a person's mental or physical activity reaches a certain level. This means that the original working ability of the human body is temporarily deteriorated and may be a symptom indicating that the body develops into a damaged state.

If fatigue persists, chronic conditions can lead to chronic fatigue syndrome. Chronic Fatigue Syndrome (CFS) is a major symptom of chronic fatigue syndrome. Long-term fatigue is associated with nonspecific symptoms such as mild fever, headache, sore throat, muscle articular pain, attention deficit disorder, memory impairment, sleep disorder and depression. (Holmes GO, et al., Amm. Intern Med, 1988, 108 (3)). The causes of fatigue include sleep deprivation, lack of exercise, unbalanced diet, bad habits such as alcoholism, mental pressure, bad working environment, and workplace environment.

There are several approaches to relieve fatigue including how to take a break, find a satisfying job, regular exercise, change to a better diet, and adequate sleep. However, it is difficult to eliminate physical and physiological fatigue in the short term by performing specific fatigue resolution methods. Therefore, it is required to develop a product that helps to overcome the physical and mental fatigue, especially, the immunity reduction, the nervous system and the endocrine system disorder, the abnormal blood vessel system and the digestive disorder. However, Although drugs with the same arousal effect are used as drugs for fatigue improvement, they do not have the effect of reducing fatigue substances in blood.

Insects have been frequently used as herb medicines since about 30 years ago, and about 30 species such as slugs, silkworms, cicadas, caterpillars, and centipedes have been used as useful insect resources. Currently, seven kinds of food materials such as white spotted flowers, It is listed in food circulation. Insects have an average protein content two times higher than that of meat, rich in minerals, essential and non-essential amino acids, vitamins, and most of the fat is composed of unsaturated fats. Nutrients contained in insects can easily be absorbed by humans, and they are emerging as a protein substitute for livestock products.

Grubby is a larva of beetles, and larvae of insects, mainly chafer and stag beetle. It is known that not only the larvae of cicadas but also the larva of other insects have the same medicinal effect as the grubs. The term grub is used as a generic term for beetle or beetle insect larvae, and commercially available insect larvae And is being used. Recently, what is commonly referred to as a 'slug' is the larva of the white spotted flower ( Protaetia brevitarsis ) or the larva of the Allomyrina dichotoma . White spider mite larvae are slightly flat at 17-24 mm in body length, with glossy black copper-tinged, yellowish-white pattern. Longevity beetle larvae are gray or grayish brown. In recent years, some farmers are raising a large amount of slugs for medicinal or edible use.

According to the 'Dongbibogam', slugs are used to treat diseases such as liver cancer, liver cirrhosis, hepatitis, cumulative fatigue, etc., which are caused by human liver, such as sight deceleration, cataracts, malignant swelling, stomatitis, tetanus and paralysis It is said that it works.

Black ginseng is produced in a similar manner to red ginseng and is dark brown compared to red ginseng. It is known that black ginseng has a higher saponin content than red ginseng and contains a large amount of effective saponins not found in white ginseng and red ginseng. Among them, ginsenoside-Rg ₃ content is much higher than that of white ginseng or red ginseng which is not cooked.

Ogapi is a deciduous broad-leaved shrub belonging to Araliaceae. Near the roots, the thorns split a lot and spread all over. The base is gray brown, has no hair, has little visible, and the leaves are elliptical in egg shape and the tip gradually becomes sharp. It is distributed throughout Korea and grows in the mountains. Ogphi muscle is used as a medicinal herb in ancient times and it is known to have the efficacy of soy sauce, interpreting gods, middlemen, gourmets, and bloodshed.

Angelica is a perennial oriental herb that belongs to the dragonfly family. It helps the metabolism and endocrine function of the body, improves blood circulation, and promotes appetite through vitality enhancement. Antioxidant, anti-obesity, anti-thrombotic, anti-inflammatory activity, and cognitive ability.

Seokchangpo is a plant belonging to the genus Chrysanthemum and iris. It is known to have sedative, memory and dementia prevention, eye protection and hair loss prevention effects.

It is a perennial herb that belongs to the genus. It is known to be effective in dementia and mental stabilization, facilitating sputum, and effective for amnesia, heart palpitations, insomnia, and irritable symptoms.

The white blooms are pine roots embedded in the sclerotium of the Poria cocos Wolf , which is parasitic on the root of pine, and the color is white. It is said to be a red hybrid when it comes to pink. It is a medicinal herb that strengthens the spleen, protects the herb, removes the moisture, helps metabolism and stabilizes the mind.

Mushrooms are part of the fungus and most of the terminal fungi belong to the fungi. It is a high-protein low-calorie food, rich in minerals and dietary fiber, and has immune function and blood pressure control.

The present inventors studied various physiological activities possessed by various insects and plants, and found that a composition in which an enzyme-treated product of the slugs and an extract of each of black ginseng, ginseng, angelica, japonica, And thus the present invention has been completed.

Korean Patent No. 10-1346884 (Name of invention: composition for prevention and improvement of anti-fatigue, inflammatory bowel disease using maca extract as an active ingredient Applicant: Pulmuone Holdings Co., Ltd. Registration date: December 24, 2013) Korean Patent No. 10-1735151, entitled Anti Fatigue Composition, Formulation and Uses thereof, Applicant: Shanghai Innovative Research Center of Traditional Chinese Medicine, Date of Registration: May 04, 2017) Korean Patent No. 10-1120996 (a composition for promoting athletic performance and improving fatigue including ginsenoside Rg3 and Rg2, applicant: Daedong Korea Ginseng Co., Ltd., registered on Feb. 21, 2012) Korean Patent No. 10-1382400 (Title of the invention: Composition for prevention and treatment of inflammatory diseases containing white spotted flowers as an active ingredient, Applicant: Korea, registered on Apr. 1, 2014) Korean Patent Laid-Open No. 10-2010-0121907 (the name of the invention: Grasshopper syrup and its manufacturing method, Applicant: Park, Eun Won, Publication date: November 19, 2010) Korean Patent Laid-Open No. 10-2016-0122110 (entitled "Immune Enhancement or Anti-Fatigue Composition Containing Fermented Placenta Composition as Active Ingredient and Its Use, Applicant: LG Household Health Co., Ltd., Publication Date: October 21, 2016) Korean Patent No. 10-0483328 (Title of the Invention: Composition Having Fatigue Recovery Function, Method for Producing the Same, and Fatigue Recovery Agent Using the Same Applicant: Chito Isoam Co., Ltd., Registered Date: Apr. 06, 2005) Korean Patent No. 10-1947786 (Name of invention: Food composition containing stress-relieving, fatigue recovery or athletic performance enhancing composition including herbal medicine extract, applicant: Kang Su-yong, registered on Feb. 07, 2019) Korean Registered Patent No. 10-1783549 (Name of invention: Composition for DHA-containing fatigue recovery enhancing supplement composition and its preparation method, Applicant: Lee, Won-Bok, Registered on September 25, 2017) Korean Patent No. 10-1513240 (Title of the Invention: composition for the preparation of a composition containing a herbal medicine mixture as an active ingredient, applicant: Kyung Kyung Kyung, registered on Apr. 13, 2015) Korean Patent No. 10-1804613, entitled Anti-stress composition using dumpling bead moth powder, Applicant: Jeju Technopark, Foundation Date: Nov. 28, 2017) Korean Patent No. 10-1315319 (Name of the invention: Food composition containing red ginseng extract and uroguan extract, Applicant: Jeju Techno Park Foundation, Registration date: Sep. 30, 2013)

Adam TC, Epel ES. Stress, eating and the reward system. Physiology and Behavior. 2007. 91 (4): 449-458. Fitts et al., Am J Physiology. 1976, 23 (2): 430-433. Yoon Sung-Ran et al 6, 2005. Changes in physicochemical properties of Korean ginseng according to heat treatment conditions. Journal of the Korean Society of Food Science and Nutrition 34 (10): 1572-1578.

It is an object of the present invention to provide a stress relieving or anti-fatigue composition containing a slug enzyme-treated product.

The present invention relates to a composition for stress relief, anti-fatigue or endurance strengthening comprising a slug enzyme-treated product, a black ginseng extract, an acerola extract, a Angelica keiskei koidz. Extract, an asian extract, a Seokchangpo extract, a bacitracin extract and a mushroom extract.

Wherein the composition comprises 20 to 30% by weight of the slug enzyme-treated, 15 to 30% by weight of the black ginseng extract, 10 to 25% by weight of the ginseng extract, 5 to 25% by weight of the ginseng extract, 5 to 15% 5 to 15% by weight of a white bacillus extract, and 5 to 15% by weight of a mushroom extract.

The slugs may be selected from one or more species selected from the group consisting of larvae of white spotted flowers and non-spider mites.

The slug enzyme-treated product may be one obtained by treating at least one enzyme selected from the group consisting of Flavozyme, Alcalase and Protease in the slug as a raw material sample.

The Ogakis may be at least one selected from the group consisting of Acanthopanax nucifera, Royal Oak Acanthopanax, Acanthopanax sp.

The mushroom may be selected from the group consisting of mushroom, mushroom, mushroom, oyster mushroom and mushroom.

The present invention also provides a stress-relieving, anti-fatigue or endurance-enhancing health functional food comprising a slug enzyme-treated product, a black ginseng extract, an acacia extract, a Angelica keiskei koidz. Extract, a chrysanthemum extract, a Seokchangpo extract, a Leptospirosis extract and a mushroom extract.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for relieving stress, anti-fatigue or endurance, which comprises a slug enzyme-treated product, a black ginseng extract, an acerola extract, an Angelica keiskei keiskei extract, a raw extract, a Seokchangpo extract, a Leek bacillus extract and a mushroom extract. The slugs that become the raw material of water may be the white spotted flowers or the larvae of long-lived beetles. At this time, white spotted flowers or larvae of long-lived beetles can be used alone or mixed together. When the larvae of the white spotted flowers or the long-lived beetles are mixed together, they can be mixed and used in a weight ratio of 1: 0.1 to 9, preferably 1: 0.5 to 2.

Wherein the composition comprises 20 to 30% by weight of the slug enzyme-treated, 15 to 30% by weight of the black ginseng extract, 10 to 25% by weight of the ginseng extract, 5 to 25% by weight of the ginseng extract, 5 to 15% 5 to 15% by weight of a white bacillus extract, and 5 to 15% by weight of a mushroom extract.

At this time, if the slug enzyme-treated product and the black ginseng extract in the composition are less than 20% by weight, the content of the useful ingredient contained therein may be lowered and the stress relaxation, anti-fatigue or endurance enhancement effect may be lowered. %, The content of the other components may be reduced and it may be difficult to exhibit the synergistic effect due to the mixing of the respective components in the liver protective effect. If the extract is less than 10% by weight or the extracts of Angelica keiskei, Chrysanthemum extract, Seokchonpo extract, Leptospirosis extract and Mushroom extract are less than 5% by weight, the activity of each extract may not be exhibited and liver protection effect may be lowered. Is more than 25% by weight or the extract of Seokchonpo, Lebblex extract and Mushroom extract is more than 15% by weight, the synergistic activity effect depending on the combination with other ingredients may not be exhibited.

The slug enzyme-treated product may be one obtained by treating an enzyme selected from Flavozyme, Alcalase or Protease in a slug, which is a raw material sample, and using an alkalase as the enzyme Most preferred.

The slug enzyme-treated material is prepared by adding 200 to 400 parts by weight of purified water based on 100 parts by weight of a slug as a raw material sample, heating and pulverizing at a pressure of 1.1 to 1.5 kgf / cm ² and at 110 to 150 ° C for 0.5 to 2 hours, The enzyme is added in an amount of 0.1 to 10 parts by weight on the basis of 100 parts by weight of the slugs and the mixture is subjected to enzyme treatment at 40 to 60 ° C for 5 to 10 hours and then the mixture is concentrated to a sugar content of 20 to 30 Brix and spray- .

In this case, spray drying is preferably carried out at a temperature of 70 to 90 ° C with a spraying pressure of 1.5 to 1.7 kgf / cm 2 and a rotation speed of 7,000 to 10,000 rpm.

On the other hand, the inactivated enzyme of the slugs is inactivated through the spray drying, so that no separate inactivation process is performed.

When the purified water is less than 200 parts by weight based on 100 parts by weight of the slime during the enzyme treatment, the quality of the proteolytic composition may be deteriorated due to over-activation of the enzyme during the subsequent enzyme treatment. When the purified water exceeds 400 parts by weight If the enzyme is further treated, the enzyme activity is weak and the protein decomposition may not be performed well.

If the heating temperature is less than 110 ° C, the sterilizing action may not occur. If the heating temperature exceeds 150 ° C, a change in the property of the protein may occur And the quality of the proteolytic composition may deteriorate. Likewise, sterilization may be difficult even if the heating condition is less than 0.5 hour, and if the heating time exceeds 2 hours, the property of the protein may be changed.

If the enzyme treatment temperature is less than 40 캜, the enzyme activity may be hardly exhibited, and if it exceeds 60 캜, the enzyme may be inactivated and not function.

When the enzyme is added in an amount of less than 0.1 part by weight on the basis of 100 parts by weight of slugs, the enzyme activity may hardly be exhibited. When the enzyme is added in an amount exceeding 10 parts by weight, The properties of the final proteolytic product may be worse.

In this case, the slug, which is a raw material sample, is used for fasting and discharging the excrement completely. It is preferable to heat the slug after washing with purified water after it is fresh and then add pure water. After the heating process is sufficiently performed, It is preferable to carry out a hydrolysis process by wet grinding and enzymatic treatment. At this time, it is also possible to carry out a process of removing oil accumulated in the slugs (larva) before or after the enzymatic treatment of the slugs or before spray drying thereof.

The black ginseng is firstly steamed at 80 to 100 ° C for 1 to 3 hours, dried at 50 to 70 ° C for 10 to 20 hours, further cooled at 110 to 130 ° C for 4 to 8 hours, Deg.] C for 10 to 20 hours, and may contain 11-13 mg / g of ginsenoside Rg3.

The components of black ginseng extract, ginseng extract, angelica gigantis extract, ginseng extract, extract of Seokchangpo, extract of Leucyeongbuk, extract of Leptospira, or extract of mushroom, which are contained in the composition of the present invention, Alcohol or a mixed solution thereof may be used as a solvent. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. At this time, it is preferable to use water as a solvent.

The solvent may be used in a volume or weight of 1 to 40 times, preferably 5 to 40 times, by volume or weight based on the weight of the raw material samples, such as black ginseng, ogapi, angelica gigas, raw paper, The extraction conditions of the extracts of each of the above-mentioned extracts of each of the above-mentioned extracts of black ginseng, gingko ginseng, angelica gingiva, native ginseng, Seokchangpo, white ginseng or mushroom may be from 20 to 100 ° C for 1 minute to 48 hours. The above process can be repeated 1 to 4 times.

As a conventional method in the art, water, C1-C4 alcohol or a mixed solution thereof extract of black ginseng, ogapi, Angelica gigas Nakai, raw paper, Seokchangpo, It may be further fractionated by using at least one solvent selected from the group consisting of chloroform, ethyl acetate and n-butanol.

The extracting device or the fraction thereof may be extracted by a conventional extracting device, an ultrasonic pulverizing extractor or a fractionating device. The extract or fraction thus prepared can be removed by hot air drying, vacuum drying or freeze drying. The extract or fraction may be purified by using column chromatography.

The extract or fraction may be isolated or isolated by known methods used for the isolation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), partition with hexane and water, And may be fractionated or purified by a suitable combination method.

The chromatography can be carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

In addition, the present invention provides a pharmaceutical composition comprising a slug enzyme-treated product, a black ginseng extract, an acanthopanax extract, a angelica ganoderma extract, an extract of leafhopper, an extract of Seokchangpo, a white bacillus extract and a mushroom extract. The composition containing the enzyme-treated product and each extract may be used as a raw material of the pharmaceutical composition or may be contained in an amount of 0.001 to 30% by weight.

The pharmaceutical compositions may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to conventional methods. Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The dosage of the pharmaceutical composition of the present invention will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection. Since the liver protecting composition of the present invention has little toxicity and side effects, it can be safely used for long-term administration for preventive purposes.

In addition, the present invention provides a health functional food for stress relief, anti-fatigue or endurance enhancement containing slugs enzyme-treated, black ginseng extract, acerola extract, Angelica gigantosa extract, earth extract, Seokchangpo extract, do. The composition containing the enzyme-treated product and each extract may be directly used as a food, but may be added in an amount of 0.001 to 30% by weight to the health functional food of the present invention. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various kinds of drinks, meat, sausage, bread, Snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamin complexes.

The present invention relates to a composition containing a slug enzyme-treated product, a black ginseng extract, an acanthopanum extract, a guangusu extract, an extract of leaf extract, a extract of Seokchangpo, a extract of Leptospira bacillus, and a mushroom extract. Fatigue efficacy or endurance enhancement efficacy, and can be used as a refined food or an additive for restoring physical strength such as juvenile, adolescent, examinee, elderly, and recovery patients.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Preparation of each powder mixture >

Example 1-1. Preparation of slug enzyme-treated products

Three larvae of white speckled larvae and three larvae of long-lived beetles were purchased from professional breeding farms and fasted for 3 days, and the larvae in the raw state, in which the excreta were completely discharged, were washed three times with running water.

Thereafter, each larva was placed in a booster, followed by addition of purified water having a weight of 3 times, and the mixture was heated at a high pressure of 1.1 to 1.5 kgf / cm ² and a temperature of 110 to 150 ° C for 90 minutes, , NEYZSCH, Srlb Bavaria, Germany) under the conditions of a bead size of 0.4 mm and a rotor speed of 3,000 rpm to produce pulverized material for each larva. Alcalase (Subtilis Carlsberg, Novozymes) was added to each of the larva larvae in an amount of 1 part by weight based on 100 parts by weight of each larva and hydrolyzed at 40 ° C for 5 hours. The hydrolyzed hydrolyzate of each larva was concentrated to have a sugar content of 20 to 30 Brix. The inlet temperature of the spray dryer was set to 170 ° C., the outlet temperature was set to 75 ° C., the spraying pressure of the spray dryer was 1.5 kgf / , And the disk rotating speed was set at 10,000 rpm. The powder thus prepared was called an enzyme-treated slug enzyme (an enzyme-treated product of a white spotted flower larva and an enzyme-treated product of a long beetle larva).

Examples 1-2. Manufacture of Black Ginseng Extract, Ogphi Extract, Angelica gigantosa Extract, Origin Extract, Seokchangpo Extract, White Bicycle Extract or Mushroom Extract

(1) Manufacture of black ginseng

First, for the production of black ginseng, the ginseng cultivated at Geumsan was purchased at Kyungdong market (Seoul, Jejudo). The purchased fresh ginseng was washed and shaken for 24 hours, and then it was firstly boiled at 95 ° C for 3 hours and then dried in a far-infrared ray dryer at 60 ° C for 15 hours. The first and second dried and ginseng were mixed again at 115 ℃ for 6 hours and dried in a far - infrared dryer at 60 ℃ for 15 hours to obtain black ginseng.

The ginsenoside Rg3 content was analyzed for the fresh ginseng, the first ginseng fresh ginseng, and the second ginseng ginseng. The ginsenoside Rg3 was not present in the unripe white ginseng, but in the first ginseng and dried ginseng, Rg3 was found to be 0.25 mg / g and ginsenoside Rg3 was found to be 11.48 mg / g in black ginseng. The ginsenoside Rg3 content of black ginseng was confirmed to be 11 ~ 13 mg / g by repeatedly preparing black ginseng and analyzing ginsenoside Rg3 content by referring to the analysis method of Yoon Sung Ran et al. (2007).

(2) Preparation of black ginseng extract, ginseng extract, angelica ginseng extract, gojiwon extract, Seokchangpo extract, white bacillus extract or mushroom extract

(Ginseng, gojiwo, goji, seokchangpo, whitbokshin, and gyeonggi mushroom) were purchased from Kyungdong Market (Seoul, Jejudo) and used as an extractive raw material together with the black ginseng prepared above.

Distilled water having a weight of 10 times the weight of the extracted raw materials was added to the extractive materials of black ginseng, gochujagi, angelica, raw paper, seokchangpo, white bacon and gingko mushroom, and the resulting liquid was filtered at 90 ° C for 12 hours. Each of the extracts was made into a powder state by setting the temperature at 75 캜, the spraying pressure of the spray dryer at 1.5 kgf / cm 2, the spinning speed of the disk at 10,000 rpm and spray drying.

≪ Example 2: Preparation of mixture >

Each powder sample prepared in Example 1 was mixed as shown in Table 1 below to prepare 100 g of mixed powder. At this time, the powders of the black ginseng extract, Ganoderma lucidum extract, Angelica keiskei koidz. Extract, Chrysanthemum morifolium extract, Seokjangpo extract, Leptospira extract and Ganoderma lucidum extract were used.

Raw material
sample The enzyme-treated product (g) of the white spotted flower larva of Example 1-1 The enzyme-treated product (g) of long-lived beetle larva of Example 1-1 Black
extract
(g) Thorn
Ogaki extract
(g) Angelica
extract
(g) Origin
extract
(g) Seokchangpo extract
(g) Whitebot extract
(g) wisdom
Mushroom extract
(g) total
(g) Example 2-1 20 0 20 10 10 5 10 10 15 100 Example 2-2 0 20 30 25 5 5 5 5 5 100 Example 2-3 30 0 20 10 10 5 5 15 5 100 Examples 2-4 0 30 15 10 5 15 15 5 5 100 Example 2-5 10 10 20 10 25 5 5 5 10 100

≪ Comparative Example 1 > Preparation of Grubu Powder and Brown Dung Larval Powder Not Performed with Enzyme Treatment [

In the same manner as in Example 1-1, white spotted flower larvae and longevity beetle larvae were powdered, and white spotted flower larvae and longevity beetle larvae which had not undergone enzymatic treatment after each larva was heated and wet pulverized .

≪ Comparative Example 2: Preparation of comparative mixture >

Each powder sample prepared in Example 1 and Comparative Example 1 was mixed as shown in Tables 2 and 3 to prepare 100 g of mixed powder.

Raw material
sample The enzyme-treated product (g) of the white spotted flower larva of Example 1-1 The enzymatic treatment of the long-lived beetle larva of Example 1-1
(g) Black ginseng extract
(g) Thorn
Ogaki extract
(g) Angelica extract
(g) Origin Extract
(g) Seokchangpo extract
(g) Whitebot extract
(g) wisdom
Mushroom extract
(g) total
(g) Comparative Example 2-1 50 0 0 8 8 8 8 8 10 100 Comparative Example 2-2 0 50 0 8 8 8 8 8 10 100 Comparative Example 2-3 0 0 40 10 10 10 10 10 10 100 Comparative Example 2-4 32 33 5 5 5 5 5 5 5 100 Comparative Example 2-5 0 0 0 15 15 15 15 15 25 100 Comparative Example 2-6 50 50 0 0 0 0 0 0 0 100 Comparative Example 2-7 0 0 100 0 0 0 0 0 0 100

≪ Comparative Example 3 > Preparation of comparative mixture using non-enzyme-treated water &

Each of the powder samples prepared in Example 1 and Comparative Example 1 were mixed as shown in Table 3 below to prepare 100 g of mixed powder.

Raw material
sample Comparative Example 1
White spotted flower larvae powder (g) Comparative Example 1
beetle
Larva powder
(g) Black
extract
(g) Ogaki extract
(g) Angelica
extract
(g) Origin
extract
(g) Seokchangpo extract
(g) Whitebot extract
(g) Mushroom extract
(g) total
(g) Comparative Example 3-1 30 0 20 10 10 5 5 15 5 100 Comparative Example 3-2 0 30 15 10 5 15 15 5 5 100

Experimental Example 1. Preparation of experimental animals and sample administration [

Experimental Example 1-1. Breeding of experimental animals

Male Sprague-Dawley rats weighing 170 ~ 180 g were purchased from Korea BioLink (Korea). The breeding environment was maintained at 20 ± 2 ℃, 55 ± 1% (RH) and 12 hours interval of light cycle, and circulated for 1 week. The feed was given a fixed amount of PMI Nutrition International for a certain period of time, and the water was allowed to freely use sterilized water using a water bottle.

Experimental Example 1-2. Sample administration

The feed for the test substance administration was granulated and administered. For this purpose, a solid feed (PMI Nutrition International) for laboratory animals was ground to a size of 70 mesh with a roll mill and then granulated to a granule having a degree of 3.0 kg / cm2 using a reverse granulation machine and dried. 2 and Comparative Example 3 were also granulated using the above-described reverse rotation granulator.

The granules prepared from the powders of Example 2, Comparative Example 2 or Comparative Example 3 were bred for 2 weeks or 4 weeks with unrestricted intake of the granules to the experimental animals (8 per each group) on the basis of the thus prepared general feed granules.

<Experimental Example 2: Confirmation of anti-stress effect>

Experimental Example 2-1. Constraint stress induction and blood collection

Constraint stress was applied to the 2 week group of each composition.

The method of applying restraint stress is to apply an antistress composition to each animal (Example 2, Comparative Example 2 or Comparative Example 3) for 2 weeks in a transparent case (40 cm x 30 cm x 30 cm) : The case was put on a vibrator for 1 hour at 00 and then vibration (2 km / hr) was performed for 7 days. At this time, each anti-stress composition was also administered during the period of restraint stress.

After 10 minutes of restraint stress for 7 days, each experimental animal was anesthetized and sacrificed, and an assay sample (serum or plasma) was collected. As a biomarker for evaluating the antistress effect in the following experiment, cortisol ), Lactic acid, glucose, and creatine phosphokinase were measured.

Experimental Example 2-2. Comparison of the amount of cortisol in the blood

When stress is induced, the body secretes cortisol to lower the metabolic rate, stimulating the cerebral appetite center to act as a food intake without hunger (Adam TC, Epel ES, Stress, and the reward system. and Behavior. 2007. 91 (4): 449-458).

Table 4 shows the amount of cortisol in the blood of animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered after induction of restraint stress.

Composition administration conditions Post-restraint stress
Serum cortisol content (㎍ / ㎗) General group (no stress, no composition treatment) 12.37 ± 1.80 Non-administration (induction of restraint stress only, no composition treatment) 43.51 + - 4.32 Example 2-1 15.42 + - 2.43 Example 2-2 16.52 + - 1.23 Example 2-3 18.78 + - 2.42 Examples 2-4 17.45 + 0.12 Example 2-5 14.57 ± 2.40 Comparative Example 2-1 28.53 + - 2.52 Comparative Example 2-2 26.60 + - 2.70 Comparative Example 2-3 29.67 + - 2.30 Comparative Example 2-4 26.74 ± 2.09 Comparative Example 2-5 38.65 ± 3.20 Comparative Example 2-6 29.12 + - 5.67 Comparative Example 2-7 32.09 ± 2.45 Comparative Example 3-1 32.32 ± 0.18 Comparative Example 3-2 33.23 + - 1.41

As shown in Table 4, the maximum amount of cortisol in the group treated with granules prepared from the powder of Example 2 was as low as 15.42 ± 2.4 3 ㎍ / ㎗, and 43.51 ± 4.3 2 ㎍ / ㎗ of the group only induced by restraint stress Respectively.

Experimental Example 2-3. Comparison of amounts of lactic acid in blood

Physical stress causes fatigue by accumulating lactic acid in muscles, and the accumulation of lactic acid involves microbial LDH (lactate dehydrogenase), which catalyzes the production of lactic acid from pyruvic acid (Fitts et al., Am J Physiology. 2); 430-433).

Table 5 shows the amounts of lactic acid in the blood of animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered after induction of restraint stress.

Composition administration conditions Post-restraint stress
Blood lactate content (㎍ / ㎗) General group (no stress, no composition treatment) 5.28 ± 1.10 Non-administration (induction of restraint stress only, no composition treatment) 45.01 + - 3.82 Example 2-1 15.63 + - 2.53 Example 2-2 18.03 + - 2.43 Example 2-3 19.23 ± 1.98 Examples 2-4 17.31 + - 4.90 Example 2-5 16.42 ± 2.78 Comparative Example 2-1 31.06 ± 2.48 Comparative Example 2-2 29.45 ± 3.90 Comparative Example 2-3 32.74 ± 2.89 Comparative Example 2-4 35.62 + - 2.33 Comparative Example 2-5 29.42 + - 4.34 Comparative Example 2-6 28.26 ± 3.97 Comparative Example 2-7 27.42 + 2.07 Comparative Example 3-1 31.23 + - 4.40 Comparative Example 3-2 29.12 ± 5.12

As shown in Table 5, the granules prepared from the powder of Example 2 were reduced to a maximum of 15.63 ± 2.53 ㎍ / ㎗, which was much lower than that of the group only induced by restraint stress, of 45.01 ± 3.8 2 ㎍ / ㎗ .

Experimental Example 2-4. Comparison of the amount of glucose in the blood

The concentration of glucose in blood has been reported to increase in experimental animals subjected to restraint stress (Korean Patent No. 10-1804613, Anti-stress composition using dumpling beads mash powder).

Table 6 shows the amount of glucose in the blood of animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered after induction of restraint stress.

Composition administration conditions Post-restraint stress
Blood Glucose Content (/ / ㎗) General group (no stress, no composition treatment) 10.23 ± 2.05 Non-administration (induction of restraint stress only, no composition treatment) 26.21 + - 3.02 Example 2-1 11.68 + - 2.47 Example 2-2 13.46 + - 2.34 Example 2-3 12.32 ± 3.32 Examples 2-4 14.12 ± 2.31 Example 2-5 13.34 ± 2.41 Comparative Example 2-1 19.12 ± 3.33 Comparative Example 2-2 17.75 ± 3.29 Comparative Example 2-3 18.74 + - 2.75 Comparative Example 2-4 18.58 + - 2.36 Comparative Example 2-5 21.62 ± 2.67 Comparative Example 2-6 19.12 + - 4.12 Comparative Example 2-7 18.82 + - 2.23 Comparative Example 3-1 21.21 ± 3.11 Comparative Example 3-2 19.41 + - 2.34

As shown in Table 6, the blood glucose level of the group treated with the granules prepared from the powder of Example 2 was reduced to a maximum of 11.68 ± 2.47 ㎍ / ㎗, and compared with 26.21 ± 3.02 ㎍ / ㎗ of the group only induced by restraint stress Respectively.

EXPERIMENTAL EXAMPLE 2-5. Comparison of amounts of creatine phosphokinase

It has been reported that the concentration of creatine phosphokinase in blood was increased in experimental animals subjected to restraint stress (Korea Patent No. 10-1315319, food composition containing red ginseng extract and uroguan extract).

Table 7 shows the amount of creatine phosphokinase in the blood of animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered after induction of restraint stress.

Composition administration conditions Post-restraint stress
Blood creatine phosphokinase (mU / ml) General group (no stress, no composition treatment) 32.23 + - 2.65 Non-administration (induction of restraint stress only, no composition treatment) 109.22 + 14.02 Example 2-1 45.45 ± 3.87 Example 2-2 45.46 ± 3.85 Example 2-3 49.32 + - 5.42 Examples 2-4 48.12 + - 4.35 Example 2-5 47.34 + - 4.41 Comparative Example 2-1 75.12 ± 3.37 Comparative Example 2-2 61.75 ± 3.29 Comparative Example 2-3 72.74 + - 4.77 Comparative Example 2-4 71.58 ± 3.34 Comparative Example 2-5 80.32 + - 3.67 Comparative Example 2-6 75.12 + - 4.12 Comparative Example 2-7 62.82 + - 4.23 Comparative Example 3-1 79.86 + - 4.11 Comparative Example 3-2 75.41 + - 5.34

As shown in Table 7, the granulocyte-treated group of creatine phosphokinase was reduced to 45.45 ± 3.87 mU / ml, and the resting stress-induced group was 109.22 ± 11.02 mU / ml Compared to the previous year.

<Experimental Example 3: Confirmation of anti-fatigue effect>

Experimental Example 3-1. Swimming exercise test

A swimming exercise load test was performed on groups of 4 weeks of each composition. In the swimming exercise test, the weight of 5% weight was placed on the back of the throat, and then it was taken in the constant temperature water tank and the total swimming time immediately after the exhaustion judgment (when the nose was submerged for 5 seconds) . Samples (serum or plasma) were sacrificed immediately after draining.

The duration of exercise and the amount of glucose and lactic acid in the blood were measured to evaluate the anti-fatigue effect.

Experimental Example 3-2. Swimming time comparison

The swimming time of the experimental animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered was measured and is shown in Table 8 below.

Composition administration conditions Swimming time (min) Non-administration (no composition treatment) 57.63 + - 2.67 Example 2-1 47.50 ± 2.76 Example 2-2 45.24 + - 2.53 Example 2-3 41.33 + - 1.32 Examples 2-4 46.31 + - 1.12 Example 2-5 45.30 ± 2.42 Comparative Example 2-1 25.08 + - 1.32 Comparative Example 2-2 27.87 ± 3.40 Comparative Example 2-3 28.47 ± 3.75 Comparative Example 2-4 28.59 ± 3.46 Comparative Example 2-5 21.68 ± 3.11 Comparative Example 2-6 28.67 + - 4.21 Comparative Example 2-7 27.73 + - 2.31 Comparative Example 3-1 21.45 + - 5.34 Comparative Example 3-2 23.63 + - 1.12

As shown in Table 8, in the group treated with the composition of Example 2, physical fatigue was eliminated compared with the other treatment groups, and it was confirmed that exercise time was increased.

EXPERIMENTAL EXAMPLE 3-3. Comparison of the amount of lactic acid in the blood

Table 9 shows the results of measurement of lactic acid concentration in the blood after swimming in the experimental animals to which each composition (Example 2, Comparative Example 2 or Comparative Example 3) was administered.

Composition administration conditions Lactic acid (/ / ㎗) Non-administration (no composition treatment) 1.67 ± 1.12 Example 2-1 2.32 ± 0.53 Example 2-2 2.86 ± 0.17 Example 2-3 2.32 ± 1.32 Examples 2-4 2.42 ± 1.12 Example 2-5 2.13 + - 0.34 Comparative Example 2-1 4.98 ± 1.11 Comparative Example 2-2 3.96 ± 1.90 Comparative Example 2-3 3.56 ± 1.89 Comparative Example 2-4 3.22 ± 1.33 Comparative Example 2-5 4.03 ± 1.32 Comparative Example 2-6 4.97 + - 0.41 Comparative Example 2-7 4.56 ± 1.31 Comparative Example 3-1 4.42 0.75 Comparative Example 3-2 4.45 ± 0.56

As shown in Table 9, in the group treated with the composition of Example 2, physical fatigue was eliminated compared to the other treatment groups, and it was found that the amount of increase of lactic acid caused by lack of oxygen supply in the blood was effectively suppressed.

<Experimental Example 4: Test for taking-up>

For each composition of Example 2, Comparative Example 2, and Comparative Example 3, 5 g of each of the compositions was administered to 40-60 year old male and female athletes for 20 days and 3 times a day for 1 hour after a meal. After 20 days of fatigue The degree of improvement is shown in Table 9 in comparison with that of each composition in the 5-point recurring method (5 points: excellent, 4 points: excellent, 3 points: normal, 2 points: poor, 1 point: very poor) . Table 10 shows the sleeping effect, the helplessness improvement effect, and the overall fatigue improvement effect.

division Sleep effect Helplessness improvement effect Fatigue improvement effect Example 2-1 4.2 4.3 4.6 Example 2-2 3.8 4.4 4.3 Example 2-3 4.1 3.9 4.2 Examples 2-4 4.2 4.4 4.3 Example 2-5 4.2 4.2 4.2 Comparative Example 2-1 3.0 2.9 3.0 Comparative Example 2-2 3.1 3.1 3.0 Comparative Example 2-3 3.0 2.9 3.1 Comparative Example 2-4 3.2 2.8 3.2 Comparative Example 2-5 2.4 3.3 2.9 Comparative Example 2-6 3.1 3.2 3.1 Comparative Example 2-7 3.1 3.0 3.2 Comparative Example 3-1 2.8 2.8 2.8 Comparative Example 3-2 2.4 2.9 2.0

As shown in Table 10, in the group treated with the composition of Example 2, it was found that the sleeping effect, the helplessness improving effect and the fatigue improving effect were more effective than the Comparative Example 2 and the Comparative Example 3-administered group.

These results indicate that the composition of the present invention has excellent stress relaxation efficacy, anti-fatigue efficacy, or endurance enhancement efficacy. Through these functions, it is possible to provide a composition for the treatment of various diseases such as adolescents, adolescents, examinees, It can be confirmed that it can be used as a refined food or an additive for restoring physical strength.

&Lt; Formulation Example 1 >

Formulation Example 1-1. Manufacture of tablets

200 g of the powder mixture of Example 2-1 of the present invention was mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. 10% gelatin solution was added to the mixture, followed by pulverization and passed through 14 mesh. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

<Formulation Example 2: Food Preparation>

Formulation Example 2-1. Manufacture of cooking seasonings

The powder mixture of Example 2-1 of the present invention was added to the cooking sauce at 1% by weight to prepare a cooking sauce for health promotion.

Formulation Example 2-2. Manufacture of flour food products

The powder mixture of Example 2-1 of the present invention was added to wheat flour at 0.1% by weight, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare a health improving food.

Preparation Example 2-3. Manufacture of soups and gravies

Health enhancing soup and juice were prepared by adding 0.1% by weight of the powder mixture of Example 2-1 of the present invention to soup and juice.

Formulation Example 2-4. Manufacture of dairy products

The powder mixture of Example 2-1 of the present invention was added to milk in an amount of 0.1% by weight and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 2-5. Vegetable juice manufacturing

0.5 g of the powder mixture of Example 2-1 of the present invention was added to 1,000 ml of tomato juice or carrot juice to prepare vegetable juice for health promotion.

Formulation Example 2-6. Manufacture of fruit juice

0.1 g of the powder mixture of Example 2-1 of the present invention was added to 1,000 mL of apple juice or grape juice to prepare fruit juice for health promotion.

Claims (6)

An anti-fatigue or endurance-enhancing composition comprising a slug enzyme-treated product, a black ginseng extract, an acacia extract, an Angelica keiskei koidz. Extract, an extract of green tea, an extract of Seokchangpo, a white bacillus extract and a mushroom extract. The method according to claim 1,
Wherein said slugs are selected from at least one species selected from the group consisting of white spider mite larvae and long-lived beetle larvae. The method according to claim 1,
The composition for stress relieving, antipyretic, or endurance strengthening according to claim 1, wherein the slug enzyme-treated material is one or more kinds of enzymes selected from the group consisting of Flavobia, Alkalase and Protease in the slug. An anti-fatigue or endurance-enhancing health functional food characterized by containing a slime enzyme-treated product, a black ginseng extract, an acerola extract, a ginseng extract, an extract of oriental japonica, an extract of Seokchangpo, an extract of Baekbyeon and a extract of mushroom. 5. The method of claim 4,
Wherein said slugs are larvae of white spotted flowers or long-lived beetles, wherein said slugs are for stress relief, anti-fatigue or endurance-enhancing health functional food. 5. The method of claim 4,
Wherein the slug enzyme-treated product is a stress-relieving, anti-fatigue, or endurance-enhancing health supplement, characterized in that the slug, a raw material sample, is treated with an enzyme selected from the group consisting of Flavoja, Alkalase and Protease.