KR20160137079A - A composition for promoting stem cell proliferation - Google Patents

Abstract

The present invention relates to a composition for promoting stem cell proliferation. The composition for promoting stem cell proliferation comprises: one or two or more selected from the group consisting of fucoidan, coenzyme Q10, and a snail extract; and a fermented placenta.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for promoting stem cell proliferation,

The present invention relates to a composition for promoting stem cell proliferation.

Our body is made up of about 210 different cells. Each of these cells is responsible for life activities while maintaining their unique characteristics. However, each cell has a life span and is constantly dying and replaced with new cells. Stem cells are the cells that are the origin of the cells that make up this body, and they produce cells that are necessary for the human life activity in a very small amount. Therefore, various studies have been conducted to restore body function through stem cell proliferation responsible for these functions.

Stem cells can be divided into two types: adult stem cells present in various types of tissues in our body and embryonic stem cells derived from embryos that are the beginning of human life. Adult stem cells are extremely small amounts in our body and provide the minimum number of cells needed to maintain a healthy state at all times. Since embryonic stem cells originate from fertilized embryos (embryos), there are various ethical problems in research, since they are human cells.

Under these circumstances, the present inventors have been required to study natural material materials effective for stem cell proliferation.

Tissue Engineering and Regenerative Medicine 2010, 7 (5), 592-598

The present invention provides a composition for promoting stem cell proliferation.

According to one embodiment of the present disclosure, one or more than one selected from the group consisting of fucoidan, coenzyme Q10, and snail extract; And a fermented placenta.

According to one embodiment of the present invention, the weight ratio of 1 or 2 selected from the group consisting of fermented placenta, fucoidan, coenzyme Q10 and snail extract is 100: 0.1 to 15. To provide a composition for accelerating.

According to one embodiment of the present invention, there is provided a composition for promoting stem cell proliferation comprising fucoidan, coenzyme Q10 (coenzyme Q10), snail extract, and fermented placenta.

According to one embodiment of the present invention, the weight ratio of the fermented placenta to fucoidan, coenzyme Q10 and snail extract is 100: 0.1 to 5: 0.1 to 5: 0.1 to 5, ≪ / RTI >

According to one embodiment of the present invention, there is provided a pharmaceutical composition comprising the stem cell proliferation promoting composition.

According to one embodiment of the present invention, there is provided a food composition comprising the stem cell proliferation promoting composition.

According to one embodiment of the present invention, there is provided a cosmetic composition comprising the stem cell proliferation promoting composition.

According to an embodiment of the present invention, there is provided a quasi-drug composition comprising the stem cell proliferation promoting composition.

The composition for promoting stem cell proliferation according to the present invention promotes human-derived mesenchymal stem cells, dermal keratinocyte proliferation, and p63 expression, and thereby has an immune enhancing effect.

In addition, the composition for promoting stem cell proliferation according to the present invention has an effect of inducing fatigue recovery in an individual feeling fatigue.

FIG. 1 is a graph showing the promoting effect of proliferation of mesenchymal stem cells according to Examples and Comparative Examples of the present invention.
FIG. 2 is a graph showing the promoting effect of proliferation of keratinocyte stem cells according to Examples and Comparative Examples of the present invention.
FIG. 3 is a graph showing the stem cell maintenance effect by promoting p63 expression according to Examples and Comparative Examples of the present invention.

Hereinafter, the present invention will be described in more detail.

According to one embodiment of the present disclosure, at least one selected from the group consisting of fucoidan, coenzyme Q10, and snail extract; And a fermented placenta.

According to one embodiment of the present invention, the fucoidan is a sulfated polysaccharide having a sticky, viscous structure, and is a substance having a base sugar and a sulfate group bonded thereto. The fucoidans may be commercially available or purchased from cultivated or harvested in the natural environment. For example, seaweed, kelp, lupine, Sugar Kelp ( Laminaria saccharina), L. Dizzy Tata (L. digitata), Pacific Sargassum (Fucus distichus , Okazawa mozuku ( Cladosiphon okamuranus ), but the present invention is not limited thereto.

According to one embodiment of the present invention, the coenzyme Q10 (coenzyme Q10) is a vitamin-like factor called ubiquinone, vitamin Q and the like as one of the coenzymes that function in the body. The coenzyme Q10 may be purchased commercially or may be derived from natural sources.

According to one embodiment of the present disclosure, the snail is a subspecies belonging to the gastropod or Fruticiola sieboldiana, and about 20,000 species are known in the world. The main nutritional elements of snails are known as protein (54.29%), lipid (4.18%), carbohydrate (30.45%) and ash (4.07%) and especially rich in protein and calcium. In addition, it contains Mucin, allantoin, collagen, elastin, glycolic acid, hyaluronic acid and so on. In particular, the constituent of sticky mucus secreted from snails is called mucin, and the main constituent of mucin is chondroitin sulfate.

According to one embodiment of the present invention, the snail extract may be obtained by purchasing a commercially sold snail extract or cultivated or harvested in the natural environment. For example, the snail extract may mean a liquid component obtained by immersing a desired substance in various solvents and then extracting the mixture at room temperature or a predetermined temperature for a certain period of time, and a solid component obtained by removing the solvent from the liquid component have. In addition, in addition to the above-mentioned results, the present invention can be broadly interpreted to include all of the diluted solution, the concentrated solution thereof, the adjusted product thereof, and the purified product. The method for obtaining the snail extract is not particularly limited as long as an extract having a stem cell proliferation effect can be obtained. For example, the snail is immersed in a solvent, An extraction method by heating at 40 to 100 캜, an ultrasonic extraction method by ultrasonic wave extraction, a reflux extraction method using a reflux condenser, and the like.

According to one embodiment of the present disclosure, the placenta may refer to a structure that mediates the exchange of substances necessary for the survival and growth of the fetus between the fetus and the mother.

According to one embodiment of the present disclosure, the fermented placenta may be purchased by fermenting a commercially sold placenta, or may be a commercially available fermented placenta, but the present invention is not limited thereto. The fermentation method can be used without limitation as long as it is used in the art for the fermentation of the placental composition. For example, the fermented placenta can be obtained by fermenting the placenta of a mammal as a fermenting microorganism.

According to one embodiment of the present invention, in the case of the fermented placenta, the amino acid content and the physiological activity are increased as compared with the non-fermented placenta, and the disgusting odor peculiar to the placenta is greatly reduced (Korean Patent Laid-Open Publication No. 2011-0017742).

In the present specification, 'stem cells' are cells that are the basis of cells or tissues constituting an individual, and their characteristics can be self-renewed by repeated division and differentiate into cells having specific functions depending on the environment Which is capable of differentiation. This occurs in all tissues during the developmental process of the fetus and is found in some tissues in which cells are actively replaced such as bone marrow and epithelial tissues. The functions of stem cells can be easily understood from the development of the fetus after fertilization. A fertilized oocyte is a totipotent stem cell because it can produce all the cells that make up the human body. The embryo forms a blastocyst through several cell divisions. The sacrum has the structure of the outer cell layer, the inner cell layer and the empty space around it. The extracellular layer of the cervix forms the supporting tissue such as the placenta necessary for fetal development in the uterus, and the inner cell layer forms all the tissues constituting the human body. The inner cell layer is differentiated into cells that constitute endoderm, mesenchymal, and ectodermal cells. In the present invention, the type of stem cells is not particularly limited.

According to one embodiment of the present invention, the weight ratio of at least one selected from the group consisting of fermented placenta, fucoidan, coenzyme Q10, and snail extract is 100: 0.1 to 15, preferably 100: 0.5 to 9.

According to one embodiment of the present invention, the stem cell proliferation promoting composition includes fucoidan, coenzyme Q10, snail extract, and fermented placenta.

According to one embodiment of the present invention, the weight ratio of the fermented placenta, fucoidan, coenzyme Q10, and snail extract is 100: 0.1 to 5: 0.1 to 5: 0.1 to 5, : 0.5 to 3: 0.5 to 3: 0.5 to 3.

When the concentration of the fermented placenta, fucoidan, coenzyme Q10 and snail extract is lower than the above ratio, the physiological activity is hardly exerted and the functional level is not significantly different from that of the fermented placenta alone. If the ratio is higher than the above-mentioned range, the quality of the composition will be adversely affected due to odor, moisture absorption or discoloration of the raw material itself.

According to one embodiment of the present invention, there is provided a pharmaceutical composition comprising the stem cell proliferation promoting composition.

According to one embodiment of the present disclosure, the pharmaceutical composition may be in the form of a solution, a gel, an emulsion, a suspension, a micro-emulsion, a microcapsule, a liposome, a cream, , A lotion, an ointment, an aerosol, a spray, a paste, and a patch.

The pharmaceutical composition according to the present invention may be used as a single agent or may be prepared by using a pharmaceutical agent which is known to have an approved stem cell proliferative effect and may be formulated using a pharmaceutically acceptable carrier or excipient May be prepared in unit dosage form or may be manufactured by intrusion into a multi-dose container.

As used herein, "pharmaceutically acceptable carrier" may mean a carrier or diluent that does not disturb the biological activity and properties of the compound being injected, without stimulating the organism. The type of the carrier that can be used in the present specification is not particularly limited, and any carrier conventionally used in the art and pharmaceutically acceptable may be used. Non-limiting examples of the carrier include saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and the like. These may be used alone or in combination of two or more. The carrier may comprise a non-naturally occuring carrier.

In addition, if necessary, other conventional additives such as an antioxidant, a buffer and / or a bacteriostatic agent can be added and used. A diluent, a dispersant, a surfactant, a binder, a lubricant, Pills, capsules, granules or tablets, and the like.

The pharmaceutical compositions according to the present disclosure may comprise a pharmaceutically effective amount of a fermented placenta, coenzyme Q10, fucoidan, and a snail extract.

As used herein, a "pharmaceutically effective amount" means an amount sufficient to induce a stem cell proliferation effect at a reasonable benefit / risk ratio applicable to medical treatment, and is generally in the range of 0.001 to 1000 mg / kg, May be administered in an amount of 0.05 to 200 mg / kg, more preferably 0.1 to 100 mg / kg, once or several times per day. For purposes of the present invention, however, the specific therapeutically effective amount for a particular patient will depend upon the nature and extent of the reaction to be achieved, the specific composition, including whether or not other agents are used, the age, weight, Sex and diet of the patient, the time of administration, the route of administration and the rate of administration of the composition, the duration of the treatment, the drugs used or concurrently used with the specific composition, and similar factors well known in the medical arts.

According to one embodiment of the present invention, the pharmaceutical composition comprising the aforementioned composition for promoting stem cell stimulation promotes mesenchymal stem cell proliferation (Fig. 1) and plays an important role in skin defense function Promoted the proliferation of keratinocyte stem cells (Fig. 2), promoted the expression of p63, which is known as a retention marker of keratinocyte stem cells as an intracellular transcription factor, The maintenance effect can be increased (Fig. 3).

That is, the composition promotes the proliferation of mesenchymal stem cells, keratinocyte stem cells, and p63 expression, and thus can exhibit stem cell proliferation and stem cell maintenance.

According to one embodiment of the present disclosure, the pharmaceutical composition promotes 120-150% proliferation of human-derived mesenchymal stem cells.

According to one embodiment of the present disclosure, the pharmaceutical composition promotes 120-160% proliferation of human-derived keratinocyte stem cells.

According to one embodiment of the present disclosure, the pharmaceutical composition promotes the expression of p63 by 140 to 210%.

In this specification, 'mesenchymal stem cells' are cells capable of differentiating into various musculoskeletal cells such as chondrocytes, adipocytes, muscle cells and fibroblasts as well as bone cells. In the case of mesenchymal stem cells, the regeneration of neural tissue, the induction of insulin secretory cell differentiation, the treatment of diabetes, the promotion of marrow engraftment by the simultaneous injection of mesenchymal stem cells in patients with bone marrow transplantation, In the case of reflux esophagitis, the promotion of mesenchymal stem cells and the promotion of proliferation of mesenchymal stem cells and biologically compatible macromolecule stem cells is very promising Application Research Division, 2008; 70 ~ 75).

According to one embodiment of the present invention, there is provided a method for proliferating stem cells comprising the step of administering the pharmaceutical composition to a subject.

As used herein, 'individual' may refer to any animal, including humans, that may or may not require stem cell proliferative potential. The animal may be, but is not limited to, a mammal such as a cow, a horse, a sheep, a pig, a goat, a camel, a nutrient, a dog, a cat,

In the present specification, the preventive or therapeutic method may specifically include administering the composition to a subject in a pharmaceutically effective amount.

As used herein, " administration " means introducing the pharmaceutical composition of the present invention to a patient by any suitable method, and the administration route of the composition of the present invention may be administered through various routes of oral or parenteral administration, ≪ / RTI >

The pharmaceutical composition according to the present specification can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer an amount that can achieve the maximum effect in a minimal amount without causing side effects, and can be readily determined by those skilled in the art.

The mode of administration of the pharmaceutical composition according to the present specification is not particularly limited and may be conventionally used in the art. As a non-limiting example of such a mode of administration, the compositions may be administered orally or parenterally. The pharmaceutical composition according to the present invention can be manufactured into various formulations according to the intended administration mode.

The frequency of administration of the pharmaceutical composition according to the present invention is not particularly limited, but it may be administered once a day or divided into several doses.

According to one embodiment of the present invention, there is provided a food composition comprising the stem cell proliferation promoting composition.

According to one embodiment of the present invention, since the food composition has been used for a long time as a natural substance and its stability has been proved, it can be prepared in the form of a foodstuff capable of promoting stem cell proliferation.

According to one embodiment of the present disclosure, the food composition may be a health functional food composition.

In the present specification, the term health food refers to a food having a high medical effect, such as a food for special health use (FoSHU), which has been processed so that a biological control function appears efficiently in addition to nutrition. The food may be prepared in any one form selected from the group consisting of powder, granule, tablet, capsule, liquid, and pills to obtain a useful effect for stem cell proliferation. However, Can be used without restrictions.

According to one embodiment of the present disclosure, the food composition may further comprise a pharmaceutically acceptable carrier.

In the present specification, the above-mentioned 'pharmaceutically acceptable carrier' may be applied to the above-mentioned 'pharmaceutically acceptable carrier'.

According to one embodiment of the present invention, there is no particular limitation on the type of food to which the food composition can be added, and examples thereof include various drinks, gum, tea, vitamin complex, and health supplement foods. The food composition may contain other ingredients that do not interfere with the stem cell proliferation effect, and the kind thereof is not particularly limited. For example, various herbal medicine extracts, food-acceptable food-aid additives or natural carbohydrates, such as ordinary foods, may be added as an additional ingredient.

The food-aid additive is added to produce a health functional food of each formulation and can be appropriately selected and used by those skilled in the art. For example, various kinds of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, , A stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like, but the kind is not limited by the above examples.

At this time, the content of the composition for promoting stem cell proliferation contained in the food composition is not particularly limited, but may be 0.01 to 100% by weight, more preferably 1 to 80% by weight based on the total weight of the food composition .

When the food composition is contained in a beverage, it may be contained in a proportion of 1 to 30 g, preferably 3 to 20 g based on 100 ml. In addition, the composition for promoting stem cell proliferation may further comprise additional components that can be used in food compositions to improve odor, taste, and visual appearance. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid and the like. In addition, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn) and copper (Cu) It may also include amino acids such as lysine, tryptophan, cysteine, valine, and the like. In addition, it is also possible to use antiseptics (such as potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate), disinfectants (such as bleaching powder and highly bleached white powder, sodium hypochlorite), antioxidants (butylhydroxyanilide (BHA), butylhydroxytoluene BHT), etc.), coloring agents (such as tar pigments), coloring agents (such as sodium nitrite and sodium acetic acid), bleaching agents (sodium sulfite), seasoning (such as MSG sodium glutamate), sweeteners (such as hypoglycemia, , Food additives such as flavorings (vanillin, lactones, etc.), swelling agents (alum, potassium hydrogen D-tartrate), emulsifiers, emulsifiers, thickeners, encapsulating agents, gum bases, foam inhibitors, ) Can be added. The additives are selected according to the type of food and used in an appropriate amount.

According to one embodiment of the present invention, a health functional food comprising the food composition is provided.

The health functional food according to the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients which are conventionally added in the art. Also, unlike general medicine, there is an advantage that there is no side effect that can occur when a medicine is used for a long time by using food as a raw material, and it is excellent in portability.

According to one embodiment of the present invention, there is provided a cosmetic composition comprising the stem cell proliferation promoting composition.

According to one embodiment of the present disclosure, there is provided a cosmetic comprising the cosmetic composition.

Cosmetics manufactured by using the cosmetic composition according to the present invention as an effective grammar may be prepared in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleansing foam, a soap, a body wash, and the like.

The cosmetic composition may further contain, in addition to the cosmetic composition according to the present invention, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Any of those commonly used in ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics ≪ RTI ID = 0.0 > cosmetic < / RTI >

Cosmetics comprising the cosmetic composition according to the present disclosure may contain a relatively high concentration of the composition of the present invention in the case of a wash-off type cosmetic such as a make-up remover, a cleanser, etc. in which the active ingredient remains on the skin in a short period of time It will be possible. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence, etc. in which the active ingredient remains on the skin for a long period of time, the cosmetic composition containing the cosmetic composition at a lower concentration than the wash- It will be acceptable.

According to one embodiment of the present invention, there is provided a composition for a quasi-drug, which comprises the stem cell proliferation promoting composition.

According to an embodiment of the present invention, a pharmaceutical product comprising the composition for quasi-drug is provided.

As used herein, "quasi-drugs" refers to fibers, rubber products or the like, which are used for the purpose of treating, alleviating, treating or preventing diseases of humans or animals, weak acting on the human body or acting directly on the human body, Used for the purpose of diagnosing, curing, alleviating, treating or preventing diseases of human or animal, which are not machinery, similar products, products for sterilization, insecticide and similar uses for the prevention of infectious diseases. Machinery, or apparatus, and that is not an apparatus, machine or apparatus of an article used for the purpose of causing pharmacological effects on the structure or function of a person or animal. The quasi-drug may also include external preparations for skin and personal hygiene products. But is not limited to, a disinfectant cleaner, a shower foam, a guarine, a wet tissue, a detergent soap, a hand wash, or an ointment.

The quasi-drug according to the present invention may be used as it is or may be used in combination with other quasi-drugs or quasi-drugs, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Manufacturing example  One. Stem Cells  Preparation of composition for promoting proliferation ( Example  1 to 3, Comparative Example  1 to 6)

Placenta and fermented donepezil were purchased from Horus, Japan. Fucoidan powder was used as a powder for the ganoderma lucidum extract, coenzyme Q10, and snail extract, and the following ingredients were used to promote stem cell proliferation A composition was prepared.

Cell culture medium treatment concentration (ppm) Normal
Money placenta Fermentation
Money placenta Fucoidan
powder Coenzyme
Q10 snail
extract Comparative Example 1 - - - - - Comparative Example 2 130 - - - - Comparative Example 3 - 130 - - - Comparative Example 4 - - 1.5 Comparative Example 5 - - - One Comparative Example 6 - - - - One Example 1 - 130 1.5 - - Example 2 - 130 1.5 One - Example 3 - 130 1.5 One One

Experimental Example  One. Stem Cells  By the composition for promoting proliferation Mesenchymal stem cells  (mesenchymal stem cells)

Human mesenchymal stem cells were purchased from Cellntec, Inc. in a 48-well plate (6 × 10 ³ cells / well), supplemented with BPE (Bovine), similar to fetal bovine serum The cells were cultured for 24 hours at 5% CO ₂ and 37 ° C using CNT-57 medium (Cellntec) supplemented with pituitary extract. Thereafter, the culture medium was removed through a suction tube, and then the medium was removed using a PBS solution (GibcoBRL). The CNT-57 medium without BPE was treated as in Examples 1 to 3 and Comparative Examples 1 to 6 And cultured for 72 hours at 5% CO ₂ and 37 ° C.

The cultured mesenchymal stem cells were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 assay is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, produced by the decomposition of tetrazolium salt.

Cells were spectrophotometrically measured by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. The effect of promoting the proliferation of keratinocyte stem cells of the complex composition was determined from the measured absorbance value by the following formula (1) relative to the control group (CNT-57 medium supplemented with BPE), and the proliferation rate 1.

[Equation 1]

Cell number (%) = (absorbance of sample-treated group / absorbance of control group) x 100

As a result, as shown in Fig. 1, the composition for promoting stem cell proliferation (Example 3) containing the fermentation placenta, coenzyme Q10, fucoidan, and snail extract in the proliferation ability of mesenchymal stem cells was treated , It was found that it increased greatly.

Specifically, in Examples 1 to 3 including the composition for promoting stem cell proliferation, the proliferative capacity of mesenchymal stem cells was improved by 120 to 150% as compared with Comparative Example 1, as shown in FIG.

Particularly, compared to the case where the fermented placenta (Comparative Example 3), fucoidan (Comparative Example 4), coenzyme Q10 (Comparative Example 5), and snail extract (Comparative Example 6) were treated alone, the stem cell proliferation promoting composition 3) significantly increased the ability of mesenchymal stem cells to proliferate.

These results suggest that the composition for promoting stem cell proliferation according to the present invention may be effective for the recovery of body function by promoting the proliferation of mesenchymal stem cells.

Experimental Example  2. CCK -8 Through evaluation method Keratinocyte stem cell  ( keratinocyte  stem cells)

Skin is responsible for protecting the body from external pollutants such as various pollutants and ultraviolet rays (Exp Dermatol 2002; 11: 159-87). The protective function of the skin is mainly a skin layer composed of keratinocyte stem cells and melanocytes. Thus, keratinocyte cells in the skin play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to aging, which causes various problems due to the breakdown of the homeostasis of the skin. Thus, reactivation of aging stem cells will have the same effect as turning skin age backwards.

Human keratinocyte stem cells (Human epidermal stem cells) purchased from Cellntec were cultured in the same manner as in Experimental Example 1, and CCK-8 (Cell counting kit-8) The proliferation promoting effect of keratinocyte stem cells of the composition for promoting cell proliferation was determined by measuring the absorbance value of the keratinocyte stem cells according to the formula (1) relative to the control group (CNT-57 medium supplemented with BPE) The values are shown in FIG.

As a result, as shown in FIG. 2, a composition for stimulating stem cell proliferation (Example 3) containing the fermentation placenta, coenzyme Q10, fucoidan, and snail extract was cultured in keratinocyte stem cells , It was found that it increased greatly.

Specifically, FIG. 2 shows that Examples 1 to 3 including the composition for promoting stem cell proliferation improved the proliferative capacity of dermal horny stem cells by 120 to 160% as compared with Comparative Example 1.

In particular, the composition for promoting stem cell proliferation (Example 3), the composition for stimulating proliferation of stem cells (Comparative Example 3), the fucoidan (Comparative Example 4), Coenzyme Q10 (Comparative Example 5) ) Significantly increased the ability of keratinocyte stem cells to proliferate.

These results suggest that the composition for promoting stem cell proliferation according to the present invention can enhance the skin function recovery by promoting the keratinocyte stem cell proliferating ability.

Experimental Example  3. Keratinocyte stem cell  ( keratinocyte  stem cells) stemness ) Confirm maintenance effect

The expression level of p63 was evaluated to confirm the stem cell maintenance effect of keratinocyte stem cells cultured in Experimental Example 2. p63 is an intracellular transcription factor and is well known as a maintenance marker for keratinocyte stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.

The results of the experiment are shown in FIG. In Fig. 3, the numerical value of the increase in p63 is a multiple of the control (CNT-57 medium supplemented with BPE).

As a result, as shown in FIG. 3, a composition for stimulating stem cell proliferation (Example 3) comprising all of fermentation placenta, coenzyme Q10, fucoidan, and snail extract of p63 expression of keratinocyte stem cells And it was found to increase greatly when treated.

Specifically, according to FIG. 3, the expression levels of p63 were improved by 140 to 210% in Examples 1 to 3 including the stem cell proliferation promoting composition, as compared with Comparative Example 1.

In particular, the composition for promoting stem cell proliferation (Example 3), the composition for stimulating proliferation of stem cells (Comparative Example 3), the fucoidan (Comparative Example 4), Coenzyme Q10 (Comparative Example 5) ), The p63 expression of keratinocyte stem cells was markedly increased.

These results suggest that the composition for promoting stem cell proliferation according to the present invention may exhibit stem cell-mediated maintenance effect by promoting p63 expression of keratinocyte stem cells.

These results suggest that the composition for promoting stem cell proliferation, which comprises both fermented placenta, coenzyme Q10, fucoidan, and snail extract, has stem cell proliferation promotion and stem cell maintenance effect.

It will be apparent to those skilled in the art that this specification may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (8)

Fucoidan, coenzyme Q10, and snail extract; one or more than one selected from the group consisting of fucoidan, coenzyme Q10, and snail extract; And
A composition for stimulating proliferation of stem cells comprising a fermented placenta. The composition for stimulating proliferation of stem cells according to claim 1, wherein the weight ratio of 1 or 2 selected from the group consisting of fermented placenta, fucoidan, coenzyme Q10 and snail extract is 100: 0.1-15. The stem cell proliferation promoting composition according to claim 1, wherein the composition for promoting stem cell proliferation comprises fucoidan, coenzyme Q10 (coenzyme Q10), and a snail extract. 4. The composition for promoting proliferation of stem cells according to claim 3, wherein the weight ratio of the fermented placenta to fucoidan, coenzyme Q10, and snail extract is 100: 0.1-5: 0.1-5: 0.1-5. A pharmaceutical composition comprising the stem cell promoting composition according to claim 1. A food composition comprising the stem cell promoting composition according to claim 1. A cosmetic composition comprising the stem cell promoting composition according to claim 1. A quasi-drug composition comprising the stem cell promoting composition according to claim 1.

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