TWI746492B - An accelerator that promotes the production of glycerol derived from Staphylococcus epidermidis, an accelerator that promotes the production of antibacterial peptides derived from keratinocytes of the skin, and the use of external agents for skin protection - Google Patents

Abstract

The present invention provides a skin-protecting agent and skin-protecting external agent, which act synergistically in combination with plant-based polysaccharides, and on the skin, it has glycerin that promotes Staphylococcus epidermidis, which is a beneficial skin resident bacteria The effect of the production and the production of antibacterial peptides derived from skin epidermal keratinocytes, and the effect of improving the feeling when applied to the skin is also excellent. More specifically, the present invention provides a skin protection agent containing enterococcus spheroid lactic acid bacteria, and an external preparation for skin protection.

Description

An accelerator that promotes the production of glycerin derived from Staphylococcus epidermidis, an accelerator that promotes the production of antibacterial peptides derived from keratinocytes of the skin, and the use of external agents for skin protection Invention field

The present invention relates to a function or a series of functions that promote the production of glycerol derived from skin resident bacteria, that is, Staphylococcus epidermidis as a beneficial bacteria, and the production of antibacterial peptides derived from skin epidermal keratinocytes And to protect the skin from external pressure such as dryness, or harmful bacteria and external agents for skin protection.

Background of the invention

Symbiotic microorganisms in human skin form communities in the form of skin resident flora. As skin resident bacteria, there are Staphylococcus epidermidis, Staphylococcus aureus, coryneform bacteria, etc., and the balance of these bacteria maintains the function of protecting the skin from external damage. However, it is known that rapid temperature changes, dryness, strong detergents or medicines as environmental factors, or poor general conditions caused by physical and psychological factors, trauma, mental stress, or disease, can cause the balance of these flora to collapse. This protection function is destroyed (Non-Patent Document 1).

For example, Staphylococcus epidermidis, which is known as a beneficial bacteria for the skin, can decompose sebum to produce glycerin, which can prevent dry skin or adjust the pH of the skin surface to inhibit the growth of miscellaneous bacteria and other physical protection functions. Therefore, if the proportion of Staphylococcus epidermidis decreases, it will not be able to exert these The protective function sometimes becomes the cause of skin diseases. In order to prevent this skin disease, moisturizers, antibacterial agents, fungicides, etc. have gradually been widely used in external skin preparations. However, according to these pharmaceutical ingredients, the protective function of Staphylococcus epidermidis, which is an aerobic bacteria, may be inhibited. .

In addition, Staphylococcus epidermidis also plays a role in stimulating skin epidermal keratinocytes, promoting the production of antibacterial peptides derived from skin epidermal keratinocytes, and strengthening the biological protection function of the living body. As antibacterial peptides produced by skin epidermal keratinocytes, human β-defensin-2 (hBD2: human β-defensin-2) and human β-defensin-3 (hBD3: human β-defensin- 3), and there are reports that these antibacterial peptides exhibit antibacterial effects on Staphylococcus aureus, which is a harmful bacteria to the skin, and act as a biological barrier to protect the skin from harmful bacteria (Non-Patent Document 2).

Previously, for the purpose of promoting the proliferation of Staphylococcus epidermidis, sugars such as glucose oligosaccharides were used as an assimilation source (Patent Document 1), but because it is not only beneficial to Staphylococcus epidermidis, but also to other harmful bacteria such as Staphylococcus aureus It exhibits assimilation, so there are situations where the skin's protective function is not achieved. In addition, when sugar is applied to the skin, it often exhibits a sticky feeling, which causes other problems such as difficulty in formulating on the touch.

It is known that Staphylococcus aureus mostly exists in people with dry skin or chapped skin, or the skin of patients with atopic dermatitis. It is also known that the toxin produced by Staphylococcus aureus can cause further deterioration of the skin condition. It can be considered that in order to keep the skin surface healthy, it is important to effectively kill the Staphylococcus aureus and maintain the Staphylococcus epidermidis which has a skin protection function.

The effect of Staphylococcus epidermidis on the skin is similar to that of lactic acid bacteria or bifidus, which are beneficial bacteria in the intestines. The beneficial bacteria in the intestine are known to adjust the pH value in the intestine, inhibit the proliferation of harmful bacteria such as Clostridium perfringens or Escherichia coli, and have the protective function of the intestine.

In the intestinal tract, in order to improve the intestinal environment, it is necessary to ingest beneficial bacteria such as lactic acid bacteria or Bifidobacterium. In order to make these beneficial bacteria reach the intestine in a viable state and be fixed on it, it is necessary to select acid-resistant strains or enteric-coated Type of encapsulation, etc. In addition, in order to increase the beneficial bacteria in the intestines, a method of ingesting various oligosaccharides prepared with dietary fiber or selective assimilation of beneficial bacteria is also carried out. However, these methods may also cause excessive intestinal fermentation or diarrhea due to different personal physiques. As one of the methods for solving this problem, there is provided a method of heat-treating dead cells using spherical lactic acid bacteria (Patent Document 2).

The heat-treated dead bacteria of Enterococcus faecalis can be ingested by making food and drink, which can promote the proliferation of Bifidella in the intestines, inhibit the proliferation of harmful bacteria, and improve the intestinal protective function. In addition, since it is a dead bacteria, it is easy to process as a food and drink, and unnecessary ferment is not carried out, so it is less likely to affect the storage stability of the food and drink.

As the heat-treated dead cells of lactic acid bacteria, microorganisms classified as Enterococcus faecalis can be suitably exemplified, and it is particularly preferable to use Enterococcus faecalis EC-12 strain (accession number FERM BP-10284), Enterococcus faecalis The 16S rDNA of the EC-12 strain (deposit number FERM BP-10284) was registered as "AB15482" at the National Institute of Genetics in Japan.

Prior art literature Patent literature

[Patent Document 1] Japanese Patent Laid-Open No. 2005-002087

[Patent Document 2] Japanese Patent Laid-Open No. 2004-051530

[Non-Patent Literature]

[Non-Patent Document 1] Gallo, R.L. et al., Journal of Investigative Dermatology 131, P1974-1980 (2011)

[Non-Patent Document 2] Kisich, K.O. et al., Journal of Investigative Dermatology 127: 2368-2380 (2007)

Summary of the invention

In keeping the skin surface healthy, it is indispensable to protect the skin from external pressure such as dryness or harmful bacteria. The subject of the present invention is to provide one or a series of methods for promoting the production of glycerol derived from Staphylococcus epidermidis and the production of antibacterial peptides against Staphylococcus aureus and other harmful bacteria derived from skin epidermal keratinocytes. Function, and protect the skin from external pressure such as dryness, or harmful bacteria and external agents for skin protection.

The inventors of the present inventors investigated various lactic acid bacteria and repeatedly studied diligently. As a result, they found that the bacterial cells of Enterococcus faecalis EC-12, which is a spherical lactic acid bacteria, especially the heat-treated dead cells, have a promotion-derived surface. The effect of the production of glycerin by Staphylococcus dedermidis and the production of antibacterial peptides derived from skin epidermal keratinocytes. In addition, when applied to the skin, it is dry and has excellent tactility, and it imparts a sensory improvement effect to any type of non-water-based, oil-based, emulsion-based, and powder-based agents.

That is, the present invention includes the following items.

[1] An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, which contains Enterococcus spherical lactic acid bacteria as an active ingredient.

[2] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis as described in Item 1, wherein the above-mentioned Enterococcus globular lactic acid bacteria is Enterococcus faecalis.

[3] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis as described in Items 1 to 2, wherein the above-mentioned Enterococcus globular lactic acid bacteria is Enterococcus faecalis EC-12 (accession number FERM BP-10284).

[4] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis as described in Items 1 to 3, wherein the above-mentioned Enterococcus spherical lactic acid bacteria are dead cells.

[5] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis as described in Items 1 to 4, wherein the above-mentioned Enterococcus spherical lactic acid bacteria are heat-treated dead cells.

[6] An accelerator for promoting the production of antibacterial peptides derived from skin epidermal keratinocytes, which contains Enterococcus spherical lactic acid bacteria as an active ingredient.

[7] The promoter for promoting the production of antimicrobial peptides derived from skin epidermal keratinocytes as described in Item 6, wherein the above-mentioned Enterococcus globular lactic acid bacteria is Enterococcus faecalis.

[8] The promotion of skin epidermal keratinocytes as described in items 6 to 7 An accelerator for the production of antibacterial peptides, wherein the aforementioned Enterococcus globular lactic acid bacteria is Enterococcus faecalis EC-12 (accession number FERM BP-10284).

[9] The promoter for promoting the production of antimicrobial peptides derived from skin epidermal keratinocytes as described in items 6 to 8, wherein the above-mentioned Enterococcus spheroid lactic acid bacteria are dead cells.

[10] The promoter for promoting the production of antimicrobial peptides derived from skin epidermal keratinocytes as described in items 6 to 9, wherein the above-mentioned Enterococcus spherical lactic acid bacteria are heat-treated dead cells.

[11] The antibacterial peptide production promoter according to claims 6 to 10, wherein the antibacterial peptide is human β-defensin-2 (hBD2: human β-defensin-2), human β-defensin-3 (hBD3: human β-defensin-3).

[12] An external preparation for skin protection, which is formulated with the accelerator that promotes the production of glycerol derived from Staphylococcus epidermidis and the accelerator that promotes the production of antibacterial peptides derived from skin epidermal keratinocytes as described in Items 1 to 11.

The agent or external preparation for skin protection of the present invention can promote the production of glycerol derived from Staphylococcus epidermidis and human β-defensin-2 as an antibacterial peptide against Staphylococcus aureus derived from skin epidermal keratinocytes , The production of human β-defensin-3. That is, by promoting the production of glycerin, it can prevent dry skin, and it can also promote the regulation of the pH value of the skin surface and inhibit the growth of bacteria and other skin protection functions. On the other hand, it can promote the production of antibacterial peptides against Staphylococcus aureus derived from skin epidermal keratinocytes, and protect the skin from harmful bacteria on the skin. Therefore, through these one Or a series of promoting functions can maintain the health of the skin surface state. Furthermore, when applied to the skin, it is dry and has excellent tactility, and it can impart a sensory improvement effect to any type of external agent of water-based, oily, emulsion, and powder.

Figure 1 shows that the heat-treated dead cells of Enterococcus faecalis EC-12 and true aloe polysaccharides as plant polysaccharides are added to the culture solution of Staphylococcus epidermidis standard bacteria (ATCC12228), and the glycerin The result obtained by quantifying the amount of production.

Figure 2 shows the result of adding heat-treated dead bacteria of Enterococcus faecalis EC-12 to the culture solution of skin resident bacteria collected from the eyebrows of humans, and quantifying the amount of glycerol produced.

Figure 3 shows the results obtained by quantifying the amount of glycerin collected from human cheeks in a human observation test using a soft lotion containing enterococcus faecalis EC-12 heat-treated dead bacteria.

Figure 4 shows the cultivation of human normal keratinocyte culture strains in the medium containing the heat-treated dead cells of Enterococcus faecalis EC-12. RNA was extracted from the human normal keratinocyte strains, and human β-defense was measured by real-time PCR The results are obtained based on the expression levels of HBD-2 (hBD2) and human β-defensin-3 (hBD3) mRNA.

Detailed description of the preferred embodiment

The promotion of glycerol production derived from Staphylococcus epidermidis in the present invention An agent, an accelerator that promotes the production of antibacterial peptides derived from keratinocytes of the skin, or an external preparation for skin protection, contains Enterococcus spherical lactic acid bacteria as an active ingredient. The enterococcus spheroid lactic acid bacteria are Enterococcus faecalis, especially the heat-treated dead cells of Enterococcus faecalis EC-12 as a preferred aspect.

The medicine or external preparation for skin protection of the present invention has the following characteristics.

1. Promote the production of glycerol derived from Staphylococcus epidermidis.

2. Furthermore, by using it in combination with plant polysaccharides, it synergistically promotes the production of glycerol derived from Staphylococcus epidermidis.

3. Promote the production of antibacterial peptides derived from skin epidermal keratinocytes against harmful bacteria such as Staphylococcus aureus.

4. Through one of these or a series of promotion functions, it can protect the skin from external pressure such as dryness, or hyperfunction caused by harmful bacteria.

5. When it is applied to the skin, it is very dry and has excellent tactility, and it can provide a sensory improvement effect to any type of external agent of water-based, oily, emulsion, and powder.

The culture method of Enterococcus spheroidal lactic acid bacteria includes the previously known lactic acid bacteria culture method, and is not particularly limited. Heat-treated dead bacteria can be produced by the following method. The bacterial cells can be recovered from the culture obtained by culturing Enterococcus spherical lactic acid bacteria according to conventional methods by methods such as filtration, centrifugal separation, etc., washed with water, suspended in water, etc., heated, and then concentrated as needed. Drying, thereby preparing heat-treated dead bacteria. It can be carried out using a spray dryer, a freeze dryer, etc. that are commonly used. Depending on the situation, it can be used before and after the sterilization treatment such as heating, or Enzyme treatment, surfactant treatment, grinding or pulverization treatment is carried out before and after the drying treatment, and those obtained by these treatments are also included in the dead bacteria or its treatments of the present invention.

The water used for washing and suspension not only includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, etc., but also includes those obtained by various treatments. The treatment performed on the water includes, for example, purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, and buffering. Therefore, the water that can be used as an extraction solvent in the present invention also includes purified water, heat-treated water, ion exchange water, physiological saline, various buffers, and the like.

The enterococcus spherical lactic acid bacteria obtained by the above method can be used as an accelerator to promote the production of glycerol derived from Staphylococcus epidermidis, and the production of antibacterial peptides derived from skin epidermal keratinocytes against harmful bacteria such as Staphylococcus aureus Promoters, or external agents for skin protection that protect the health of the skin's surface state through one or a series of these promotion functions.

Preferably, the accelerator for glycerol production derived from Staphylococcus epidermidis and the antibacterial peptide production accelerator derived from skin epidermal keratinocytes of the present invention contain 5×10 heat-treated dead cells per 1 gram. ¹¹ or more, more preferably 5×10 ¹² or more, for suspension preparation. In addition, for the daily use of the external preparation for skin protection, it is preferably to be formulated and prepared in such a way that one-third to the same amount is used for each use.

(Form of external agent for skin protection)

The external preparation for skin protection of the present invention includes ampoules, capsules, powders, granules, liquids, gels, foams, emulsions, and tablets. Use appropriate forms such as 1) pharmaceuticals, 2) quasi-drugs, 3) topical or systemic skin external preparations (e.g. lotions, lotions, creams, ointments, lotions, etc.). Cosmetics, oils, facial masks and other basic cosmetics, solid soaps, liquid soaps, hand soaps and other facial cleansers or cleansers, massage agents, facial cleansers, hair removal agents, depilatory agents, shaving treatments, aftershave lotions , Shaving lotion, shaving cream, foundation, lipstick, rouge, eye shadow, eyeliner, mascara and other makeup cosmetics, perfumes, nail polish, nail polish, nail polish remover, dressing, hard ointment, patch, tablet Agents, patches, aerosols, etc.), 4) medicinal or/and cosmetic preparations applied to the scalp and hair (e.g. shampoo, cleansing agent, hair conditioner, pre-wash conditioner, styling Liquids, hair dyes, hair dressings, hair tonics, hair growth/hair care products, poultices, plasters, patches, tablets, aerosols, etc.), 5) bath agents used in bath water, 6 ) And anti-armor odor or deodorant, antiperspirant, sanitary products, sanitary napkins, wet wipes, etc.

(Components of external preparations for skin protection)

In addition, this kind of external preparation for skin protection can be produced by arbitrarily selecting and using the ingredients or additives exemplified below as required. The amount of the formulation is not specifically defined, but it is generally considered to be preferably about 0.0001-50%. .

(1) Various oils and fats

Calyx pear oil, almond oil, fennel oil, perilla oil, olive oil, sweet orange oil, Atlantic sea bream oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, shea fatty acid, macadamia nut oil, safflower Oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, Peach kernel oil, castor oil, cottonseed oil, peanut oil, tortoise oil, mink oil, egg butter, palm oil, palm kernel oil, wood wax, coconut oil, tallow, lard, squalene, squalane, pristine Or hydrogenated products (hydrogenated oil, etc.) of such fats and oils.

(2) Waxes

Beeswax, carnauba wax, spermaceti, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice bran wax, etc.

(3) Mineral oil

Liquid paraffin, petrolatum, paraffin, ozokerite , ceresin, microcrystalline wax, etc.

(4) Fatty acids

Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, hypolinoleic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid Acid, undecylenic acid, tall oil, lanolin fatty acid and other natural fatty acids, isononanoic acid, caproic acid, 2-ethylbutyric acid, isovaleric acid, 2-methylvaleric acid, 2-ethylhexanoic acid , Isovaleric acid and other synthetic fatty acids.

(5) Alcohols

Ethanol, isopropanol, lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, phenoxyethanol and other natural alcohols; 2-hexyldecanol, isostearyl alcohol, 2 -Synthetic alcohols such as octyldodecanol.

(6) Polyols

Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene two Alcohol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butanediol, Pentylene glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol, etc.

(7) Esters

Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl lauryl myristate , Hexyl decyl dimethyl octanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, acetylated lanolin, ethylene glycol monostearate, Propylene glycol monostearate, propylene glycol dioleate, etc.

(8) Metal soaps

Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

(9) Gum, sugar or water-soluble polymer compounds

Gum arabic, benzoin gum, dammar gum, guaiac resin, Irish moss, karaya gum, tragacanth gum, locust bean gum, quince seed, agar, casein, lactose, fructose , Sucrose or its esters, trehalose or its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, added with alkylene oxides such as ethylene oxide ( C ₂ ~C ₄ ) hydroxyalkyl (C ₂ ~C ₄ ) chitin or chitosan, low molecular chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin Or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfuric acid or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carboxyethyl Sodium cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylic acid Polyalkylene oxide such as ester, polyacrylate, polyethylene oxide or polypropylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine, etc.

(10) Surfactant

Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric interface Active agent: carboxylic acid type amphoteric surfactant (amine type, betaine type), sulfate type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant), other surfactants (Natural surfactants, derivatives of protein hydrolysates, polymer surfactants, surfactants containing titanium-silicon, fluorocarbon-based surfactants) etc.

(11) Various vitamins

Vitamin A group: Retinol, Retinal (Vitamin A1), Dehydroretinal (Vitamin A2), Carotene, Lycopene (Pre-Vitamin A), Vitamin B Group: Thiamine Hydrochloride, Thiamine Sulfate Vitamins (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol Vitamin C group: vitamin C acid or its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotachysterol, vitamin E group: vitamin E or its derivatives Substances, ubiquinones, vitamin K group: Phytonyl naphthalene Quinone (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), menadiol (vitamin K4), and essential fatty acids (vitamin F), meat Alkali, ferulic acid, gamma-sititol, orotic acid, vitamin Ps (rutin, erioside, hesperidin), vitamin U, etc.

(12) Various amino acids

Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, silk Amino acid, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamine, hydroxylysine, arginine, ornithine, histidine And other amino acid derivatives such as sulfate, phosphate, nitrate, citrate, or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal-based raw materials

These can be processed by conventional methods according to the type and form of the product to be added (arbitrary selection such as crushing, powdering, washing, hydrolysis, fermenting, refining, squeezing, extracting, distinguishing, filtering, drying, powdering, A combination of granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc.) is provided by any selection of various materials.

Furthermore, the solvent used for extraction can be selected after considering the purpose and type of the product provided, or the subsequent processing, etc. It is usually more desirable to use selected from water, methanol, ethanol, propanol, Isopropanol, butanol, isobutanol and other lower alcohols or water-containing lower alcohols, propylene glycol, 1,3-butanediol, glycerin and other polyhydric alcohols or water-containing polyhydric alcohols, acetone, ethyl acetate and other various organic solvents or A mixture of more than two kinds. But root Depending on the application, when it is not suitable to contain organic solvents, water can be used alone, or ethanol that can be easily removed after extraction, used alone or in any mixture with water, or it can be obtained by squeezing and extraction.

Furthermore, when the additives derived from plant or animal-based raw materials are used for whole body or topical external use, cosmetics, etc., in addition to the protection of skin or hair, the improvement of moisturizing, touch/texture, Endowment of softness, relaxation of irritation, relaxation of pressure using fragrance, cell activation (preventing cell aging), suppression of inflammation, improvement of skin/hair quality, prevention and improvement of skin chapped, hair growth, hair growth, prevention of hair loss , In addition to the beauty effects such as gloss imparting, cleansing effect, fatigue relief, blood flow promotion, warm bath effect, etc., the effects of flavor correction, deodorization, viscosity increasing, antiseptic, and cushioning can also be expected.

Furthermore, in addition to this, various cosmetic and medicinal effects of the previously known raw materials can be expected, and by combining these, it is also possible to produce a product that achieves the enhancement of the target effect of the present invention and expects a multifunctional effect.

Specific plants (natural medicines) used as raw materials include, for example, almonds (almonds), indigo (blue leaves), Qingfeng vine, coral trees, sycamore or glabrous sycamore, fennel, green gastrodia, acacia, red rose Hemp, red currant "fruit", red clover, red grape, ink tree (hematoxylin), wild sycamore (wild paulownia), red madder or western madder or madder (madder root), aka yellow ground yellow or ground yellow (Rehmannia) glutinosa), Asafoetida, East Asian Thalictrum, Pouteria gardneriana, Palmetto palm, Tongcao (Mutong), Hemp seeds, Purple morning glory or morning glory, Hydrangea (Hydrangea), Ashitaba, Red beans ( Red Adzuki bean), Lohan cypress, acerola (acerola), catechu, edible canna, anise (anise), sweetened egg yolk (abiu), abiurana, rape, cork oak ``fruit'', avocado, flax, gancha, Horse chestnut, wilt (Polygonatum japonicum), Shan Cigu (Guang Cigu), Amaranthus (Amaranthus, Yanlaihong, Amaranthus tail, Immortal Valley, Amaranthus, Green Amaranthus, Amaranthus retroflexus, Amaranthus Amaranth, green persimmon orchid, Chiho Valley), braided lily or fritillary, alstroemeria (Alstromeria), bugloss (Alkanna tinctoria), mesquite (Algarrobo), hollyhock, arnica, grass cardamom (Alpinia) katsumadai), aloe vera, true aloe vera, angelica, apricot (almond), benzoin, yolk fruit (yellow sapote), ground bile, barberry (Epimedium grandiflorum), rush, Polygonum cuspidatum (Polygonum cuspidatum root), yew , Strawberry, Fig "Fruit, Leaf", Iris (Iris tectorum), Abutilon avicennae (Mallow), Deerwort, Ginkgo "Seeds and Leaves", Polygala tenuifolia (Polygala tenuifolia), Carob, Scapula Seed (Draba nemorosa), Amaranthus blitum (Amaranthus blitum), Rice "seed, seed coat", Anethum graveolens "seed", mountain pepper (Thymus quinquecostatus), nettle, ylang-ylang, sowcory (Conandron ramondioides) , Selaginella or Rock Pine, String Beans, Oolong Tea, Fennel, Genipap Tree (Huito, Genipap), Jing Sanleng (Triangle), Oriole vine ``fruit'', Tibetan Fennel (Carum carvi), Turmeric (Turmeric), Waterweed (duckweed), Asarum or Asarum (Asarum), Mallow (Malva sylvestris), Hollyhock (Althaea officinalis), Ponerorchis graminifolia (Ponerorchis graminifolia), Prunella vulgaris, Loneola (Aralia cordata) (Qianghuo, Tang Duhuo), Cat's Claw Vine (Uncaria Tomentosa), Uva Tea, Plum (Black Plum ``Seed, Pulp''), Quercus salicina (Quercus salicina), Bear Grape Leaf, Wen State mandarin orange (chen peel), Wenpu, fragrant tansy, acanthopanax senticosus, green persimmon orchid, echinacea (echinacea), gorse (Cytisus scoparius), winter mushroom (Flammulina velutipes), cassia (Senna obtusifolia) or Jue Ming, Hercampure, elderberry (Sambucus nigra) "fruit", elemi balm, Pleurotus eryngii (Pleurotus eryngii), Sophora japonica (Sophora japonica, Sophora japonicus), pea, Astragalus, Polygonatum falcatum (Polygonatum falcatum), Queen Victoria dragon Glossa (Agave victoriae-reginae), Coptis, Cactus, Trichosanthes bracteata (Trichosanthes bracteata) (Inula helenium), Ivy (Inula helenium), Green vine (fangji), Plantago (Plantaceae), Flos Lu ( Rhaponticum uniflorum (Qizhou Leu Lu, Pulsatilla), Hazelnut (Corylus heterophylla), Benqing Zhu (Cereus giganteus), Atractylodes macrocephala, Lagerstroemia speciosa, Lushun Alnus (Alnus sieboldiana) ``fruit'', Eriocaulon buergerianum, Pouteria sapota, Crataegus, Lodoicea maldivica, Barley, Oxalis tuberosa, Cnidium monnieri, Okra "fruit", Inula britannica (inula), Echinops latifolius (blue thorn head), ginseng or bamboo joint ginseng, Hypericum perforatum or Hypericum perforatum (dicut grass), wild sesame (Diptera) ), Xanthium (Xanthium), Juglans mandshurica (Juglans mandshurica), Dioscorea or Dioscorea (薢), Gastrodia elata, Lilium lancifolium (Lilium lancifolium) or Japanese lily (Lilium japonicum) or wild Lilium brownii (lily), Chondradenia (Chondradenia) fauriei), Formononetia, Ulmus laciniata, Huanghualong Bud, Tuber Chenopodium (Olluco), Watercress (Nasturtium officinale) (Cresson), Parsley (Petroselinum crispum), Psoralea corylifolia, Dry celery (Apium graveolens), olive ``fruit, seed, leaf'', oregano (oregano), orange ``fruit, peel'', Rehmannia glutinosa Libosch (Rehmannia glutinosa Libosch), cocoa ``fruit, peel, seed'', persimmon (persimmon stalk) ``Leaf''), Uncaria (fishing vine hook), Glechoma hederacea (Lotus), Persimmon orchid, Hydrangea (Hydrangea macrophylla), Cassia, Broussonetia papyrifera (Broussonetia papyrifera) (Fruit) , Curcuma, Quercus (Valcum leaf), Cascarilla (cascarilla), American buckthorn peel (Cascara sagrada), Xia grass, Inula salicina (Inula salicina), Sea sand (Golden sand vine), Quinoa (Chenopodium pallidicaule), Carnation, valerian, birch, birch or silver birch (Betula pendula) (white birch), gerbera, pumpkin, gibe ``seed'', pepper (Zanthoxylum bungeanum) (shu pepper), cattail (tail yellow), chamomile or Roman chamomile (Roman chamomile), Tongcao, Camu fruit (Myrciaria dubia), Calla lily, Fumaria officinalis, Trichosanthes cucumeroides, Pinellia ternata, Oats, Guarana ``seeds'', Magnolia officinalis (Magnolia officinalis), Sycamore, Wolong, Papaya, Garcinia Cambogia, Agastache rugosa, Potentilla (Potentilla chinensis Ser.) (Potentilla chinensis), Veronica undulata, Yunzhi , Dianthus or dianthus (Qumaizi), Artemisia apiacea (Artemisia apiacea), Pteridium kawawara (Artemisia spp.), Kansui, Licorice, Kansui Tara agave, candelilla, coltsfoot (Tussilago farfara), canna, olive, raspberry (raspberry, Dutch berry, white berry, red plum, raspberry, red raspberry), kiwifruit, fruit , Ye'', yellow black melon (Kuolou root), platycodon grandiflorum (platycodon grandiflorum root), chrysanthemum (chrysanthemum, camomile, Korean wild chrysanthemum), camomile chrysanthemum, catalpa tree (catalpa), sheep's hooves (Bauhinia aurantium), Citrus aurantium, water chestnut often Ivy, Aloe arborescens, Campanula (Satureja), Quinine, Quinine, Quinoa (Chenopodium quinoa), Phellodendron amurense (Cork), Pigeon pea, Gymnema sylvestre, Hexagonal column cactus (Cereus peruvianus), Cabbage, whole cabbage "immature fruit", caraway, cucumber, tamarisk (West river willow), saponaria (Quillaja saponaria), Quillaja saponaria, lycopodium (a golden sore grass), kumquat "fruit", beetle tree , Camellia, Opuntia tuna, Piper betle, Agrimony (Agrimonia), Golden orchid, Silver orchid, Golden edge, Pouteria laurifolia, Guava ``fruit'', Grey Guayule (Parthenium) Argentatum), Quercus infectoria, Macadamia stone fruit, Cephalanthera longifolia, Lycium barbarum (Lycium barbarum, Lycium barbarum leaf, Geophyseal bark), Asparagus, Pueraria lobata (Kudzu root), Camphor tree, Gooseberry (Ribes uva) -crispa) "fruit", wormwood (Artemisia annua), Parashorea macrophylla, gardenia (Gardenia jasminoides), oak tree, quaint aso (Theobroma grandiflorum), citronella, beargrass, verbena, sophora flavescens, cranberry ``, chestnut ``seed, fruit, inner bark'', Curculigo Latifolia (Curculigo Latifolia) ``fruit'', grapefruit ``fruit, leaf'', buckthorn, Ilex rotunda Thunb. Clove (Ding Zixiang), Luo Han Cai (Thlaspi, Thlaspi), Nepeta (Nepeta), Cockscomb (cockscomb), Epiphyllum, Laurel, Soil Poria (Returning from the Mountain), Gentiana, Ganoderma lucidum (Geranium), Hovenia dulcis (Geranium), Dendrobium nobile (Dendrobium nobile), Citrus kinokuni (Orange, Citrus, Orange) , Fulai mandarin, Sagami mandarin, grapefruit, orange peel), rose flower, melissa officinalis (Melissa officinalis), eucalyptus ``fruit'', black tea, petunia (Sichuan bone), chaomoto, sorghum, galangal, coriander "Fruit", Citrus edulis, Scutellaria baicalensis Georgi (Scutellaria baicalensis Georgi), Bilberry, Coconut "Fruit", Evodia, Rubus chingii (Raspberry), Pepper, Cosmos, Cuban Balsam (Copaifera officinalis), Coffee ``seeds, leaves'', Xin Yi or Magnolia (Xin Yi), Burdock (Arctium lappa), Female Wilt (Clematis apiifolia), Flax, Scrophulariaceae, Schisandra, Southern Schisandra or Two-color Schisandra, Wheat, Rice or Rice Bran ``Red Bran, "White bran", rice bran oil, Sudan kola nut "seed", kola nut "seed", boxwood leaf on the thorn vine (Coronilha), fenugreek "fruit", African fangji root, South American milk rattan bark, kelp, konjac, Kang R&F, sisal fibre, picking orchid, bird's nest orchid (Hokkaido bird's nest orchid), clematis chinensis (Clematis chinensis), cherry tree (Oshima cherry, mountain cherry, Oyama cherry, Edo Higan cherry, bean cherry, deep mountain cherry, Somei Yoshino Sakura, Kaolin Sakura, Kasumi Sakura, Kohigan Sakura, Satoshi Sakura, Han Sakura (Leaf, Flower, Fruit, Bark (Sakura Bark)), Cherry, Pomegranate, Bamboo, Cephalanthera longibracteata, Camellia, Ozaia (alisma), sweet potato, sugar cane, sugar beet, wild jujube (jujube seed), saffron (saffron), sapodilla, egg yolk (Pouteria campechiana), vermilion ``fruit'', saponaria, American giant tree Raspberry "fruit", cohosh, sand blue thorn head, sage, large lobelia (lobelia), water walnut (hickory), tianchi, Peruvian dragon's blood (croton), three branches and nine leaves Grass, Notoginseng ginseng, dogwood, san pepper, mountain bean root, avocado, avocado tree (Butyrospermum parkii) ``fruit'', shiitake mushroom (shiitake), asters, winged bean, digitalis, junzi, perilla or green perilla or shiso shiso or Single-sided perilla (perilla leaf, perilla seed), red sandalwood, linden tree, magnolia officinalis (Magnolia officinalis), admiralty (forsythia), shimeji mushroom (brown gray tricholoma, smoked pleated umbrella, lotus leaf pleated umbrella, silvery white Pleurotus ostreatus, Mushroom, Jade mushroom), Purple Magnolia (Xinyi), Spiraea, Potato, Coral (Adenophora adenophorum), Jasmine (Jasmine), Eriocheir sinensis (Ophiopogon japonicus) , Long cowpea, Gypsophila paniculata, Shrink sand density (Amomum, Shrink sand), Chuan Valley, Palm "fruit", Chunlan, Queen Kwai, ginger, Changshan, Iris (Iris root), Cardamom "fruit", Green Castanopsis "seed", white butterfly "seed", white clover (clover), chicken bone grass (Abrus fruticulosus), fragrant iris (Iris florentina), white flower vine "flower", white lupine, Nandina domestica), red cinchona (Cinchona succirubra), Ruixiang (Suixianghua, Shen Dinghua), cinnamon (cinnamon), cinnamon (cinnamon), watermelon, honeysuckle (honeysuckle), sorrel, Swim berry ``fruit'', star apple, stevia , Strawberry "Fruit", Bell Bupleurum (Xu Changqing), Horsetail (Wenjing), Purslane (Portulaca oleracea), Plum "fruit", Jianlan (male orchid), European Scots pine "Fruit", European larch , Ivy, Persian walnut, Western crabapple, Western dandelion, horse chestnut (buckeye), Western pear "fruit", spiraea, European elderberry (elderberry), European juniper (juniper), Yarrow (Yarrow), Rosa rosa, Supper, European Mistletoe, Peppermint or Lemon Balm, Horseradish, Iris (Dendrobium), Dendrobium (Dendrobium Sakura, Original Spring Dendrobium, Dendrobium, Okinawa Dendrobium, Dendrobium officinale, Dendrobium butterfly, Dendrobium quadrangularis, Dendrobium huanghua) (Dendrobium (dendrobium)), Lemon Verbena (Deodorant Wood), Mallow, Polygala Broadleaf, Polygala Polygala, Cress, Essium, Ligusticum Chuanxiong, Andrographis, Tantan, Shrunken Cactus, Senna "fruit, leaf", grape leaf clematis (large Polygonum), Dangyao, Chuanpu, Caoguo, Lemaireocereus griseus, Suxin winter plum, buckwheat "seeds", dioscorea, broad beans, rhubarb, radish, soybeans, lime (orange peel, Citrus aurantium), East China blue thorn Head (Southeast Blue Spiny Head), Body Intestines (Eclipta prostrata), Euphorbia, Golden Dog Hair Fern (Dog Ridge), Black Sweet Cherry "Fruit", Hollyhock, Thyme (Thyme), Slender Dioscorea (薆), Li Willow, tamarind "seed", onion, fragrant Xinyi (Xinyi), Aralia "fruit, leaf, root bark", big-leaf holly (one leaf tea, kudingcha, bitter lantern tea), dahlia (passiflora), miscellaneous Color lupine, sputum cut bean, single spiny cactus, salvia, dandelion or white flower dandelion or Mongolian dandelion, Danma, cherry "fruit", Imperata ``fruit, root, bud'', bamboo ginseng, chicory, Phyllanthus niruri (Phyllanthus niruri) , Tulip, Chocolate roh stay, artichoke (artichoke), Korean rhubarb (rhubarb), Korean wormwood (Turtle), artichoke (wormwood), Maitake mushroom (Polyporus), grass silkworm, evening primrose, Neottia kiusiana , Agaricus bisporus (mushroom), Tutong grass, Camellia, Gotu Kola, Clawfoot grass (Suchsia), Commelina communis, Red adzuki bean, Dendrobium, Polygonum multiflorum, Apricot, Goat milk (four-leaf ginseng), Vine Beans, Ligularia, Erythrina, Ordinary Walnut, Dewberry "Fruit", Gelidium, Sweet Tea, Tiantai Wuyao (Black Medicine), Winter Melon (Winter Melon Seed), Chinese Uncaria, Chili (Pepper), Angelica, Calendula ( marigold), honey locust, Sichuan Dipsacus (Chuan Duan), corn or corn silk (Zea virginiana), Ligustrum lucidum (Ligustrum lucidum), gentian or Korean gentian, horsetail, houttuynia cordata, spit root, dodder seed or kudzu, Eucommia "bark, leaf, root", ash (Qin bark), tomato, astragalus, honey locust "seed", Eustoma, Potentilla, Liaoyang, Hibiscus, Lingling Cougars, Niger berry "fruit", sweet potato or yam, butcher'sbroom, scallion, shepherd's purse, rapeseed, concanavali, lime, jujube (jujube), golden cherry seed, dipsacea, pu mushroom , Peanut (groundnut), nandina (nandina fruit), ramie, fragrant pansy, bitter wood, absinthe, nutmeg, cinnamon or cinnamon or Ceylon cinnamon or cinnamon (cassia bark) or cinnamon stick, Orchis joo-iokiana, Leek (leek), elderberry ``fruit, flower, stem, leaf'', garlic (garlic), salt tree (gall), spring onion, albizia or albizia or hell flower, wild thistle (thistle), wild rose ( Yingshi), Bignonia (trumpet creeper), pheasant comb, yarrow, Chinese angelica (former Hu), wild rose, green peach, pineapple ``fruit'', hibiscus (Hibiscus, chandelier hibiscus, roselle), inclined Gentiana, Lupins, Glycoxylon praealtum, Quercus fern (Rhizoma Drynariae), White-flowered Yousen, Orchis aristata, White ringworm, bilberry ``fruit'', Chrysanthemum, Hazel (filbert), Scopolon (Hycopolethes root), Basil, Lotus (Lotus) Meat, lotus seeds), cilantro (parsley), rye, sweet potato, light bamboo or bitter bamboo (zhuru), god mint, mint (mint leaves), eight liters of beans, coix (coix seed), anemarrhena, banana, beef To, Flower (Tribulus, Tribulus terrestris), Vanilla Bean, Papaya, Sage, Paharobobo, Bobbu "whole grass, stem, leaf", red pepper, fire orchid, single leaf vine or three leaf Vitex (Vitex), Cyperus rotundus, Tribulus (Tribulus), Rose (Rose), Coral (Parsnip), Witch Hazel, Palm, Rose, Balata, Balata, Edible Toadstool (Horse mushroom, ball-based mushroom), aspidistra, green money willow (jujube), wall grass, spring cold orchid, bitter gourd (vine lychee), spring elm (elm bark, white bark of elm, elm leaf), nicotine spear Fruit (Lonchocarpus nicou), spring gentian, sate Real), pistachios, Sugar radish, stone reed, genistein, daisy (marguerite), hyssop, Orchis chidori, cypress, cypress leaf, castor, sunflower, sweet pepper, sky radish, cattail, Agaricus blazei Murrill, sharp-lipped bird's nest Orchid, Allspice "fruit", Angelica dahurica, Bletilla striata, Amaranth "fruit", chickpeas, lentils, hollyhock, Pulsatilla, loquat "fruit, leaf, stem", betel nut (bellied skin, betel nut), Pu'er tea (Pu'er tea), Chrysanthemum indicum (Nanteng), butterbur, coltsfoot dandelion (coltsfoot flower, coltsfoot leaf), Eupatorium (bluegrass), kidney bean (lentil), dahlia flower (Tingzi cherry, dahlia flower), Grape ``fruit, peel, seed, leaf'', beech, cordyceps (cordyceps), Brazilian licorice, Brazilian ginseng, Brazilian coconut, Brazilian ivory, blackcurrant ``fruit'', blackberry, Pradosia lactescens, plum ``fruit'', Furcellariaceae, Blueberry (Bilberry), Prune, Floral blanca, Plantain, Wendou (Mung Bean), Hazelnut, Loofah, Phoenix Lan, Safflower, Yellow Striped Agave, Belladonna, Raspberry ``Fruit'', Avocado, Peruvianberg, Sakura Kylin, Crassula, California Blackberry "Fruit", Kochia or Kochia (Kochia scoparia), Pasqueflower, Impatiens (Impatiens, Achillea, Rhododendron), Horse Rice Fruit, Egg Yolk Fruit, Japanese Magnolia, Parsnip, spinach, physalis, Magnolia officinalis (Pak), papaya, chrysanthemum (Bitter's tears), Inula sclerophylla, Atractylodes, Echinacea angustifolia, linden, peony Flowers, leaves, stems, bark'', Moutan bark, Hubu, Jojoba, Borage, Boldo leaf (Boldo), Beak berry ``fruit'', Salted Cistanche (cistanche, Dayun), Dendrobium officinale ( Dendrobium huoshanense), Araceae (Araceae), Maitake mushroom, Ephedra, Maca, Macadamia nuts, Margarita flower (Wood chrysanthemum), Partridge (Seaman grass), White mulberry (Silberry bark, bark, Leaf''), Wudang Magnolia, Balata Tree, Massaranduba rufula, Sideroxylon rugosum, Tuber Golden Lotus Flower (Tropaeolum tuberosum) (bulbar nasturtium), matata, pine cones, pine ``leaf, bark, root'', Pouteria salicifolia, Yunling (Poria cocos), Piper angustifolium (Piper angustifolium), Marjoram (cow To), Willow leaf Adenophora (Sophora flavescens), mulberry ``fruit'', quince tree, Verbascum thapsus, mango, mangosteen, chamomile (Andean chamomile), walnut tree, citrus ``fruit'', Ganoderma lucidum, Citrus aurantium "Fruit", Mishima Bupleurum (Bupleurum), Waterwheel Front (Agave), Lobelia (Lobelia chinensis), Lythrum chinensis (Lobelia chinensis), Lythrum chinensis, Nymphaea crassipes, Parsley, Lumei fruit , Spearmint, mimosa, mimosa, myrrh, myrrh, helichrysum, hibiscus, scaly tree, sapindus (long life bark), Muna (Minthostachys mollis), lilac (purple root), Purple pearl or Japanese purple pearl, purple corn, old wattle (Kunming spatholobi), Opuntia Megacantha, motherwort, basil, Melaleuca, melissa, yellow vanilla melilotus, melon ``fruit'', Mongolian wormwood, Mengzong bamboo, Muqilin , Frankincense Wood (Schinus molle), Holly Holly, Woody, Rhubarb Palmetto, Peach "Leaf, Seed, Flower, Fruit", Bean Sprouts, Morello Black Cherry "Fruit", Long Capsule Jute (Jute) , Purple sweet potato, puzzle, ghost light bark (cornflower), quasi American mint, yacon, Yaksha gall (blue cara) or weeping alder or Lushun alder "fruit, peel, ear", red bayberry, octagonal gold plate, mist Parasitic (willow parasitic), willow (gold willow, standing willow, weeping willow, glandular willow, pussy willow, cotton willow, silk willow, pointed-leaf purple willow, Yunlong willow, deep mountain willow, coconut, willow, big leaf willow, wormwood, fox Color Willow (Salix vulpina, Liao Yang), Water Willow Polygonum (Leaf, Stem), Blackberry, Purple Golden Bull, Tianmingjing (Crane Lice, Tianmingjing), Phytolacca, Liaodong Alnus (Alnus hirsuta), Bayberry (Bayberry Bark), Mugwort, Eucalyptus, Saxifraga, Yucca or Short-leaf Yucca, Pomelo "fruit", Lily, White Zhi, Ai (Artemisia argyi), Lei Wan, Lime "fruit", Rye, Luo Han Guo "fruit", Rubus "Leaf, Fruit", Peruvian Latanni (Krameria triandra), Scallion or Ashkelon Scallion (Scallion White ), lavender, longan (longan flesh), agave (green agave, agave), green tea, apple (fruit, seed, leaf, root), gentian, sweet tea rubus (rubus suavissimus), hard leaf Blue thorn head (glaze jade thistle, Xinjiang blue thorn head), lychee (lychee core), lettuce, red currant "fruit", red dragon fruit, lemon "fruit", lemon grass, admiralty (forsythia), milk vetch, winter plum , Waxed coconut, reed root (reed), big bayberry "fruit", rosemary, rose hips (wild rose), wild vegetables, sylvestris, sanguinum, etc.

Examples of seaweeds include the following as representative ones: seaweed [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidea, Enteromorpha Enteromorpha linza, Enteromorpha prolifera, Enteromorpha compressa, Enteromorpha intestinalis, Enteromorpha crinita), Ulva crinita), Seaweed [Brown algae: kelp (Laminaria japonica, Laminaria ochotensis), Laminaria religiosa, Laminaria angustata), Wakame, Wakame, Green Wakame, Giant Algae (Macrocystis pyrifera, Macrocystis integrifolia, Macrocystis integrifolia), Hijiki, Fucus, Volvox arborescens, Southern Volvox, P.australis var. cuneata, sclerophyllum spp., sclerophyllum spp., sclerotium spp., sclerotium spp., sclerotium spp.], seaweed [red algae: camellia, gelidium, flat gelidium, Japanese gelidium, chicken hairpin, pterocarpus, hard Ceratophyllum vulgaris, sclerotium cauliflower, stolonifera, cockscomb, eucheuma serrated, eucheuma opposing, eucheuma, eucheuma, carrageenan, giant carrageenan, chondrus crispus (Chondrus crispus) ), wedge-shaped carrageenan, spiny carrageenan, pinnacle carrageenan, long carrageenan, heterochromatic carrageenan, Japanese carrageenan, pinnacle carrageenan, cedar algae, red soft thorn algae, small cedar algae , Fan-shaped Acrophyllum algae, Acrophyllum acrophyllum, Acrophyllum crassifolia, Acrophyllum algae, Red leaf algae] etc.

In addition, other algae can also be listed, for example: green algae (Chlamydomonas: Chlamydomonas, Polar Snow Algae, Dunaliella: Dunaliella, Prochlorococcus: Prochlorococcus, Cercococcus: Cercococcum , Volvox: Volvox, Tetrasporium, Tetraspora, Spirogyra: Glycosporum, Spirogyra, Pseudomonas genus, Hylophyta: Silk algae, Ulva: Ulva vulgaris , Ulva Ulva, Ulva Ulva, Ulva spp. Genus: Ulva, Fischeria, Cladosporium: Cladosporium japonicum, Cladosporium sacchari, Cladosporium tulum, Cladosporium, Phyllanthus: Macrocystis Cytophytes, Phytocystis, Bananas: Bananas, Pteridophytes: Pteridophyta, Racemes, Serranophyllum, Pteridophytes, Hypophyllum, Sporphyllum: Spinosporium , Sub-tubular pine algae, constricted pine algae, long pine algae, flat pine algae, Spirulina genus: Ryukium sclerophyllum, Sclerotium sp Luna, Cladosporium, Tylosporium, Tennis algae: Tennis algae, Reef membrane genus: Reef membrane, Broad reef membrane, Guerrella reef membrane, Pachycetella, Coleoptera, Pentaphyllum, Orange Algae: Orange Algae, Double Star Algae, Nonseparous Algae, etc.).

Cyanobacteria (Cryptophyta: Suizonsi cyanobacteria, Microcystis Genus, Nostoc: Nostoc schizophylla, Nostoc common, Nostoc hair, Oscillatoria, Spirulina: Spirulina, Trichosanthes, etc.).

Brown algae (Metacystis: Mesocystis, Hydra: Indian water cloud, Synechococcus: Synechococcus, Phaeocystis spp.: Phaeocystis mycoides, Hydracea: Trigonococcus tricornutum, Seawing Algae genus: Filamentous sea wing algae, Verbena spp: Cylindric vulgare, Verbena vulgaris, Verbena scatter, Dictyostelium genus: Dictyostelium, Dictyodactylus latifolia, Dictyostelium spp: Dictyostelium, Cladophyta, Phaeophyllum genus: Phaeophyta, Dictyola genus: Dictyoptera divergentis, Dictyola glabra, Dictyola fern-shaped, Phaeophyllum genus: Phaeophyllum, Volcanophyllum genus: Tree Turquoise algae, Pleurotus grandiflora, Volvox spp, Brachychaete genus: Brevibacterium bands, Phaeochromia: Suspicious Phaeophyllum, Mucosal algae: Mucosal algae, Phaeophyte genus: Phaeophyllum, Ceratophyllum Genus: Ceratophyllum, Chlorella: Trichophyta, Cladosporium: Gangmura Cladosporium, Silk algae: Silk algae, Sphaerotheca spp: Noodle algae, Coccidiophora genus: Sclerococcus, Hydrilla verticillata Genus: Hydrilla verticillata, Acrophyllum genus: Acrophyllum, Mozuku genus: Mozuku, Iron sphagnum: Iron sphagnum, Leaf-shaped sclerotium, Carrophyllum genus: Costalaria, Trichosanthes genus: Trichosanthes, Trichophyllum genus: Tangled Tritonella, Acidophyllum genus: Tongue-shaped acid algae, Acidic acid algae, light brown acid algae, Chlorophyceum genus: Smooth chrysophyllum, Phyllostachys spp. : Chickweed, Sphaerophyllum genus: Sphaerophyllum sp ：Thousandis cysts, Dictyostelium: Dictyostelium, Trichosporium: Trichosporium, Cyclosporium: Sporophyllum, Ceratophyllum: Trichosanthes, Cyclocystis: Cyctocystis gracilis , Dayweed: Dayweed, Netsweed: Netsweed, Ropeweed: Hypophyllum, Podophyllum: Podophyllum, Polycosta: Polycosta, Podophyta: Podophyta, Japanese Podophyta , Kelp genus: leathery Kelp, Long-handed kelp, Ghost kelp, Ezo kelp, Long kelp, Endo kelp, Laminaria cichorioides Miyabe, Laminaria genus: Cyclosporium cichorioides, Laminaria genus: Pseudo laminae, Laminaria genus: Laminaria, creeping kelp, kelp, chrysanthemum Laminaria: Chrysanthemum Laminaria, Streptophyllum genus: Streptophyllum, Pseudotwistella spp.: Black Island Pseudo-wrought Algae, Arthrophyllum genus: Arthrophyllum difidum, Evisia spp.: Erwinia vulgaris, Pteranophyllum spp. , Pterocephalus, Ceratophyllum: Carrageenan, Cystophyllum: Cystella cuneata, Trumpetella: Trumpetella, Cystophyllum: Cystophyllum Hakodate, Cystophyllum, Cystophyllum turneri Yendo, Horsetail Algae: Sargassum japonicum, Sargassum hemiphyllum, Sargassum segii Yoshida, copper algae, Sargassum lineare, Sargassum asparata, Sargassum Sagami, Sargassum nigrifolium Yendo, Sargassum sphagnum, Sargassum tosa, Sargassum tosa, Sargasso, Sargassum, Sargassum rensii, Artemisiae, Sargassum japonicus, Sargasso spinosa, S. schizophylla, S. grandiflora, S. macrophylla, Zococcus spp : Macrophytes, Coelocystis genus: Coelocystis, Cyanocystis genus: Cyanocystis, etc.).

Red algae (Horata: dark purple red hair, small red hair, seaweed: laver, striped seaweed, pseudo-linear seaweed, long seaweed, Tianzuo seaweed, multicolor seaweed, laver seaweed, Rhododendron: Hawthorne Spirulina, Mycelium: Mycelium, Mycelium: Mycelium, Japanese Mycelium, Soft Mycelium, Seaskin: Seaskin, Multi-row Seaskins, Fork The genus of the sclerophyllum, the genus of Helminthosporium: Helminthosporium, the genus Helminthosporium, the genus of Dendrobium spp. Trichophyton genus: Trichophyton fragile, Carmocystis genus: Carocystis circulans, Bifidophyllum falciparum, Ptilonia genus: Ptilonia okadae, Comodontella genus: Comodontella, Salmonella: with Eucalyptus, Yew-shaped Asparagus, Gelidium: Gelidium, Gelidium, Gelidium, Gelidium, Gelidium, Hydrangea (Hydrangea macrophylla), Gelidium whisker, Glycyrrhiza ：Dudresnaya minima, Dudresnaya minima, Leptophyllum: Leptospira, Peytrium: Pithella, Gliocladium: Thickened Gliophyta, Single Gloeosporium, Zinc Genus: Irregular Zengzi, Constantinea: Constantinea subulifera, Neodilsea yendoana (Neodilsea yendoana), Neodilsea tenuipes, Softgrass: Miulifera Granules, Nigrass japonicus, Red scutellaria: Northern red scutellaria, Contarinia genus: Contarinia okamurae, Ceratocystis genus: Peyssonnelia caulifera Okamura, Ceratocystis spp.: Ceratocystis japonicus, Dioscorearium spp.: Broad flat Dendrobium, Coral Algae: Coral Algae, Centipede Algae: Asiatic Centipede Algae, Branch Centipede Algae, Dichobranch Centipede Algae, Tongue-shaped Centipede Algae, Gangcun Centipede Algae, Compound Centipede Algae, Succulent Centipede Algae, Elliptic Centipede Algae, Centipede algae, Centipede lanceolate, Centipede blackwood, Hymenophyllum species: Slimegodae, Halymenia polydactyla, Centipede centipede, Serrated algae: Serrated algae, Cypress genus: Haibo, Shield Fruit algae: Scutellaria, Scutellana narrow-branch, Scutellaria, multi-branched Scutella, Scutellaria, Scutella diversiformis, Arthropod algae, Centipede cuticles, High Centipede alga, Centipede spp., Cryptospora spp.: Centipede spp., Myxoplasma spp.: Myxotubules spp., Schimmelmannia genus: Schimmelmannia plumosa, Hydrangea spp.: Small sea snail, Hydrangea spp., Staghorn sea snail, Murphylla spp: Wall Fruit algae, beautiful leaf algae: Japanese beautiful leaf algae, curl beautiful leaf algae, palm-shaped beautiful leaf algae, clinging beautiful leaf algae, adjoining beautiful leaf algae, cockscomb beautiful leaf algae, Callophyllis hayamensis, purple beautiful leaf algae, rosacea beautiful leaf algae, beautiful leaf genus: beautiful leaf algae, aspergillus genus: aspergillus genus, sphaerotheca genus: sessile saccharomycete, Kallymenia sagamiana, Kallymenia callophylloides, Smithium: Japanese history Dense algae, T. lancifolia: Tsengia lancifolia, Smooth algae: Smooth algae, Schizophyllum: Schizophyllum, Dictyostelium: Dictyostelium, Slimeophyta: S. Yamada, The genus Red Ling: thick red Ling, weak red Ling, Cockscomb: Felt Cockscomb, Turnerella: Turnerella, Streptomyces: Streptococcus clumps, Pittospermum: Pittosporium , Cartilaginous sea-head red, Pseudomonas: Long-branched Pseudomonas, Trichosanthes, Variety Pseudomonas, Japanese Pseudomonas, Sphaerotheca spp : Sea fungus, Gracilaria: Gracilaria true, Gracilaria serrata, Gracilaria crispus, Gracilaria crassicarpa, Gracilaria vulgaris, Gracilaria platensis, Gracilaria caudate, Gracilaria stellate, Gracilaria subtidal , Gracilaria yamamoto, Gracilaria scoparium, Gracilaria, Gracilaria, Gracilaria, Gracilaria, Taro-root Gracilaria, Gracilaria lanceolata, Gracilaria cuneata, Lithospermum spp Genus: Gracilaria constricta, Pseudomonas genus: Pseudomonas sp The genus of Ezag and Brookside Fern: the genus of Brookside Fern, the genus of Cedar: Algae, Gigartina ochotensis, Pacific Cedar, Ginkgo biloba genus: Ginkgo biloba, Rhodoglossum Genus: Myxocladium japonicum, Dictyostelium: Dictyostelium spinosa, Dictyostelium peduncle, Chrysophyllum spp Botryococcus, Botryococcus: Botryococcus, Cryptosporidium: Cryptosporidium, Palmarella: Palmarella, Rhodophyllum intricate, Rhodophyton lactis, Hydrangea: Bright Hydrangea , Cytocystis genus: Endo Cytocystis, Arthrobacter spp.: Arthrobium catenum, Arthrobacter elegans, Gangcun Festival Lycopersicon esculentum and Annellia: Fluorescens, Expansional anemone, Celestial genus: Three-pointed celestial celery, soft celestial celery, cone celestial celery, Japanese celery, borden celestial celery, Nectrophyll's Vegetables, Climbing Mollia: Graceful Climbing Mollia, Renbao Algae: Shishi Renbao Algae, Red Leaf Algae: Marsella schischii, Thousand Island Dense Fruit Algae, Japanese Hymenophyllum, Acrophyta: Creeping Acrophyllum, Polyphyllum genus: Japanese Polyphyllum, Neophyllum genus: Myriogramme polyneura, Hideophyllum yezoense, Acrophyllum genus: Uncaria, fan-shaped Acrophyllum, thin veins Acremonella, Acrophyllum, Hymenena genus: Hymenena tenuis, Rhododactyla: Rhodophyta, Partridge genus: Partridge-free partridge, Chlotophyton genus: Chlotophyton sessileum, Heterophyton genus: Japanese different tube Algae, Heterophyton beautiful, Chlamydomonas genus: Chlamydomonas, Polytubular algae: Polytubularis, Fragile polytubules, Partridge: Partridge, Chondrosis: Chondrosis arborescens, Chondrosylla crassifolia, Chondrosylla arbuscularis, Chondrosylla tangutorum, Chondrus ryukinus, Chondrosis creeping, Chondrosoma vulgaris, Cotyledonella variegata: Cotyledon variegata, Cotyledon coryneformis, Cotyledon pinnae, Red feather Pterocephalus, Pterocephalus: Pterocephalus, Pterocephalus fibrosa, Dactysoma Cylindrica, Convolvulus genus: Black Convolvulus, Japanese Convolvulus, Amansia mitsuii Segawa, Chrysanthemum genus: Omura Chrysanthemum, Lechneria: Laurelella, Pymophyllum genus ：Phymenophyllum, Neopinenodula: Prickly Neopinenodularia, Odonthalia corymbifera, Leuconostoc: Leuconostoc, Leuconostoc, Leuconostoc black, Hot spring red algae: Hot spring red algae , The genus of Pseudo-Hassomen: Porphyridium, Porphyridium, Rhodophyllum: Rhodophyllum, etc.).

Trifolium (Chara, Lactophyllum, Pseudocystis spp.: Pseudocystis obliquus, Dengochia spp., Lactophyllum spp.: Lactophyllum spp., C. acuminata, Ornithola, etc.), yellow algae (Monochrophyllum spp.: Chrysophyllum rosenbergii, etc.) and so on.

In addition, as materials derived from animal-based raw materials, examples include: cockscomb extract, bovine or pig, human placental extract, bovine or pig stomach, duodenum, intestine, spleen extract or its decomposition products, bovine or pig Extracts of brain tissues, hydrolysates of collagen from cattle, pigs or fish (acid, alkali, enzymes, etc.) or collagen derivatives such as water-soluble collagen or acylated collagen, elastin or elastin from cattle or pigs Hydrolysates (acids, bases, enzymes, etc.) or water-soluble elastin derivatives, keratin and its decomposition products or their derivatives, silk protein and its decomposition products or their derivatives, porcine or bovine blood globulin Decomposition product (globulin powder), bovine or porcine hemoglobin decomposition product (chloroproheme, hemoglobin, protohemoglobin, protohemoglobin, heme iron, etc.), cow milk, casein and its decomposition products or their derivatives Products, skimmed milk powder and its decomposition products or derivatives of these, lactoferrin or its decomposition products, egg components, fish meat decomposition products, nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid), etc.

In addition, as materials derived from plant or animal-based raw materials, arbitrary parts, cells, tissues, organs, metabolites, etc. derived from genetic recombinants or cell fusions can also be used. Furthermore, it is also possible to use, for example, an undifferentiated cell group or a cell group in the middle of differentiation obtained by cell culturing an arbitrary site, cell, tissue, organ, or the like.

(14) Marine ingredients

Sea water such as deep water, such as sea salt, dried sea water, inorganic salt (sodium chloride, chlorinated Magnesium, potassium chloride, etc.), sea mud or mud (fango), such as Italian mud, German mud, Eifel mud, Freiburg mud and other sea mud or mud (containing ingredients : Silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead , Manganese, arsenic, water), sacred stone, etc.

(15) Microbial culture metabolites

Yeast metabolite, yeast extract, bacterial metabolite, bacterial extract, mold or actinomycete metabolite, mold or actinomycete extract, natto metabolite, natto extract, fermented rice extract, rice bran (red bran , White bran) fermented extract, euglena extract or its decomposition products or water-soluble derivatives of these, trehalose or its derivatives, lactic fermented product of raw milk or skimmed milk powder, fermented product of lactic acid bacteria of legumes, Lactic acid bacteria ferment of coconut plants, etc.

(16) Alpha-hydroxy acids

Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.

(17) Inorganic pigments

Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron oxide yellow, iron red, iron oxide Black, ultramarine, chromium oxide, chromium hydroxide, carbon black, hemimorphite, etc.

(18) Ultraviolet absorption/blocking agent

酯、二丙二醇水楊酸酯、乙二醇水楊酸酯、水楊酸肉豆蔻酯、水楊酸甲酯等)、鄰胺苯甲酸衍生物(鄰胺苯甲酸甲酯等)、尿刊酸衍生物(尿刊酸、尿刊酸乙酯等)、香豆素衍生物、胺基酸系化合物、苯并三唑衍生物、四唑衍生物、咪唑啉衍生物、嘧啶衍生物、二

烷衍生物、樟腦衍生物、呋喃衍生物、吡喃酮衍生物、核酸衍生物、尿囊素衍生物、煙鹼酸衍生物、維生素B6衍生物、傘形酮、馬栗樹皮苷、肉桂酸苄酯、辛諾賽、氧苯酮、二氧苯酮、辛苯酮、磺異苯酮、苯醯間苯二酚、熊果苷、癒創薁、紫草甯、黃苓苷(baicalin)、黃芩素(Baicalein)、小檗鹼、丁基甲氧基二苯甲醯甲烷、Escalol、氧化鋅、滑石、高嶺土等。 Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone Sodium benzophenone-5-sulfonate, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxydi Benzophenone, etc.), p-aminobenzoic acid derivatives (p-aminobenzoic acid, ethyl p-aminobenzoate, glyceryl p-aminobenzoate, amyl p-dimethylaminobenzoate, p-dimethylamine Octyl benzoate, etc.), methoxycinnamic acid derivatives (ethyl p-methoxycinnamate, isopropyl p-methoxycinnamate, octyl p-methoxycinnamate, p-methoxycinnamic acid 2 -Ethoxyethyl, sodium p-methoxycinnamate, potassium p-methoxycinnamate, di-p-methoxycinnamate mono-2-ethylhexanoic acid glyceride, etc.), salicylic acid derivatives (salicylic acid) Octyl acid, phenyl salicylate, high salicylic acid

Ester, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urine Acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, two

Alkyl Derivatives, Camphor Derivatives, Furan Derivatives, Pyranone Derivatives, Nucleic Acid Derivatives, Allantoin Derivatives, Nicotinic Acid Derivatives, Vitamin B6 Derivatives, Umbelliferone, Horse Chestnut Glycoside, Cinnamic Acid Benzyl esters, sinoxane, oxybenzone, dioxyphenone, octanophenone, sulfisophenone, phenylresorcinol, arbutin, guaiazulene, shikonin, baicalin (baicalin) , Baicalein (Baicalein), berberine, butyl methoxy dibenzyl methane, Escalol, zinc oxide, talc, kaolin, etc.

(19)Whitening agent

烷衍生物、樟腦衍生物、呋喃衍生物、吡喃酮衍生物、核酸衍生物、 尿囊素衍生物、煙鹼酸衍生物、維生素C或其衍生物(維生素C磷酸酯鎂鹽、維生素C葡糖苷等)、維生素E或其衍生物、曲酸或其衍生物、氧苯酮、二苯甲酮、熊果苷、癒創薁、紫草甯、黃苓苷、黃芩素、小檗鹼、胎盤萃取物、土耳其鞣酸、白藜蘆醇(Rucinol)等。 P-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, Pyrimidine derivatives, two

Alkyl derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C Glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine , Placenta extract, Turkish tannic acid, Resveratrol (Rucinol), etc.

(20) Tyrosinase activity inhibitor

Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or its derivatives (hydroquinone benzyl ether, etc.), kojic acid or its derivatives, vitamin E or Its derivatives, N-acetyl tyrosine or its derivatives, glutathione, hydrogen peroxide, zinc peroxide, placental extract, turkey tannic acid, arbutin, resveratrol, silk extract, Plant extracts (chamomile, mulberry, gardenia, angelica, elm, sophora, wormwood, honeysuckle, yellow barberry, houttuynia cordata, yunling, coix, wild sesame, mulberry, hawthorn, eucalyptus, yarrow, hollyhock , Cinnamon, vitex, witch hazel, chicken mulberry, longevity grass, platycodon, dodder, saccharum, shegan, ephedra, chuanxiong, single life, bupleurum, parsnip, coral dish, scutellaria, peony peel, yak Ermiao, kudzu root, licorice, gallnut, aloe, cohosh, safflower, green tea, black tea, Axian medicine) and so on.

(21) Melanin pigment reduction/decomposition substance

Phenylmercury hexachlorophenol, mercury oxide, mercurous chloride, hydrogen peroxide water, zinc peroxide, hydroquinone or its derivatives (hydroquinone benzyl ether), etc.

(22) Promoting metabolic turnover/cell activation substances

Hydroquinone, lactic acid bacteria extract, placenta extract, Ganoderma lucidum extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extracts (aloe, Scutellaria, wood Thieves, gentian, burdock, purple root, ginseng, witch hazel, hubu, coix seed, wild sesame, as medicine, angelica, marigold, sweet tea, forsythia, cucumber, thyme, rosemary, coriander) and so on.

(23) Astringent

Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, hemimorphite, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (containing catechin compounds), etc. .

(24) Active oxygen scavenger

SOD (Superoxide Dismutase), catalase, glutathione peroxidase, etc.

(25) Antioxidant

Vitamin C or its salt, stearate, vitamin E or its derivative, n-dihydroguaiac acid, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), hydroxytyrosol, p-hydroxybenzoic acid Ether, propyl gallate, sesamol, sesalin, gossypol, propolis, etc.

(26) Lipid peroxide formation inhibitor

β-carotene, plant extracts (flax cultured cells, glycerine tea, forsythia, witch hazel, cloves, melissa, trilobite, birch, sage, rosemary, nan Tianshi, Yingshi, ginkgo, green tea) Wait.

(27) Anti-inflammatory agent

Ichthyol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, Chamazulene, Chamazulene, Chlorpheniramine Maleate, Glycyrrhizic acid or its salt, Glycyrrhetinic acid or its salt, Licorice extract, Purple root extract, fruit extract, propolis, etc.

(28) Antibacterial, antiseptic, and disinfectant drugs

Rivanol, sulfur, calcium gluconate, chlorhexidine gluconate, aminosulfonic acid, mercury bromide, lactoferrin or its hydrolysate, chlorinated alkyldiaminoethylglycine solution, Triclosan, sodium hypochlorite, chloramine T, bleaching powder, iodine compound, iodoform, hexadienoic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, parabens, undecylenic acid, Thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, revanillol, phenethyl alcohol, o-phenylphenol, Irgasan CH3565, halocarban, hexachlorophenol, diclofenac, ethanol, methanol, isopropanol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, Zinc pyrithione, chlorobutanol, isopropyl methylphenol, non-ionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.), amphoteric surfactants , Anionic surfactants (sodium lauryl sulfate, potassium lauryl sarcosine, etc.), cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methyl chloride, etc.) Rose aniline), formaldehyde, hexamethylene tetramine, brilliant green, malachite green, crystal violet, Germal, photoreceptor 101, photoreceptor 201, photoreceptor 401, N-long-chain aniline basic amino acid Derivatives and acid addition salts, zinc oxide, juniper alcohol, sophora flavescens, propolis, etc.

(29)Humectant

Glycerin, propylene glycol, 1,3-butanediol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfuric acid or its salt, water-soluble chitin derivative, water-soluble chitosan derivative, pyrrolidone carboxylic acid or Its salt, sodium lactate, Ozasa Nishiki rice (Mini Sasanishiki) extract, natto bacteria metabolite, natto extract, loofah water, white birch or red pine sap

(30) Elastase activity inhibitor

Diisopropyl fluorophosphate, plant extracts (Scutellaria baicalensis, Forsythia suspensa, Sophora flavescens, mulberry leaf, cinnamon bark, Ganoderma sylvestris, Kang Fuli, sage, European elderberry, Linden, Moutan bark), seaweed extract, etc. .

(31) Hair agent

Selenium disulfide, alkyl isoquinolinium bromide, zinc thiopyrimidinyl, genisalate, thioxanthene, castor tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong ammonia, potassium bromate, bromic acid Sodium, thioglycolic acid, etc.

(32) Antiandrogens

Oocytes hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, isoethenolone, etc.

(33) Peripheral blood vessel promoting blood flow agent

Vitamin E or its derivatives, herbal extracts, garlic extracts, ginseng extracts, aloe extracts, gentian extracts, angelica extracts, cephalothinine, carprochloramine, mellonidine, etc.

(34) Stimulants

Capsicum tincture, vanilloid pelargonate, cantharidin tincture, ginger tincture, peppermint oil, 1-menthol, camphor, benzyl nicotinate, etc.

(35) Metabolic active agent

Photosensitive element 301, cypress alcohol, pantothenic acid or its derivatives, allantoin, placental extract, biotin, glyceryl pentadecanoate, etc.

(36) Anti-seborrhoeic agent

Pyridoxine or its derivatives, sulfur, vitamin B6, etc.

(37) Keratolytics

Resorcinol, salicylic acid, lactic acid, urea, etc.

(38) Oxidizing agent

Hydrogen peroxide water, sodium persulfate, ammonium persulfate, sodium perborate, carbamide peroxide, sodium percarbonate, sodium tripolyphosphate peroxide, sodium bromate, potassium bromate, sodium pyrophosphate peroxide, sodium orthophosphate peroxide, Sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme liquid, mushroom extract, etc.

(39) Hair removal agent

Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide; thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, Strontium thioglycolate) and so on.

(40)Hair swelling agent

Ethanolamine, urea, guanidine, etc.

(41) Dyestuff

5-amino-o-cresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'- Aminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitro-p-phenylenediamine hydrochloride, p-phenylene diamine hydrochloride Amine, N-phenyl-p-phenylenediamine hydrochloride, m-phenylenediamine hydrochloride, o-aminophenol, N-phenyl-p-phenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diamino Pyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitro-p-phenylenediamine, p-aminophenol, p-nitro-o-phenylene diamine, p-benzene Diamine, p-methylaminophenol, picramine Acid, sodium picrate, N,N'-bis(4-aminophenyl)-2,5-diamino-1,4-quinodiimide, 5-(2-hydroxyethylamino) -2-methylphenol, N-phenyl-p-phenylenediamine, m-aminophenol, m-phenylenediamine, 5-amino-o-cresol sulfate, 2-amino-5-nitrophenol sulfate, o-amine sulfate Phenol, 2-chloro-p-phenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitro-p-phenylene sulfate Diamine, p-aminophenol sulfate, p-nitro-o-phenylenediamine sulfate, p-nitro-m-phenylenediamine sulfate, p-phenylenediamine sulfate, p-methylaminophenol sulfate, m-aminophenol sulfate, meta-benzene sulfate Diamine, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucinol, gallic acid, resorcinol, tannic acid, 2-hydroxy-5-nitro Base-2',4'-diamino azobenzene-5'-sodium sulfonate, hematoxylin, etc.

(42) Spices

Natural animal flavors such as musk, civet, castoreum, and ambergris; anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, perfume tree essential oil, caraway essential oil, cardamom essential oil, Guaiacum essential oil, cumin essential oil, camphor essential oil, cinnamon essential oil, Ceylon cinnamon essential oil, geranium essential oil, Cuban balsam essential oil, coriander essential oil, perilla essential oil, cedar essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil , Cedar essential oil, spearmint essential oil, peppermint essential oil, star anise essential oil, tuberose essential oil, clove essential oil, neroli essential oil, wintergreen essential oil, tolu gum essential oil, citrifolium essential oil, rose essential oil, palmarosa essential oil, juniper essential oil , Lohan cypress essential oil, white sandalwood essential oil, bee lemon essential oil, geraniol essential oil, vetiver essential oil, citrus essential oil, Peruvian gum essential oil, rosewood essential oil, lin camphor essential oil, citrus essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, Agarwood essential oil, lemongrass essential oil, lemon Lemon essential oil, rosemary essential oil, Japanese peppermint essential oil and other plant fragrances; other synthetic fragrances, etc.

(43) Pigment, coloring agent

Red cabbage pigment, red rice pigment, madder pigment, annatto pigment, squid melanin, curcumin, sophora rice pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia pigment, corn pigment, onion pigment, Tamarind pigment, spirulina pigment, buckwheat pigment, cherry pigment, seaweed pigment, hibiscus pigment, grape juice pigment, calendula pigment, purple sweet potato pigment, purple yam pigment, shellac pigment, rutin, etc.

(44) Other

Examples include hormones, metal ion blocking agents, pH regulators, chelating agents, antiseptics/anti-mold agents, cooling agents, stabilizers, emulsifiers, animal/vegetable proteins and their decomposition products, animal/vegetable polysaccharides And its decomposition products, animal/plant glycoproteins and its decomposition products, blood flow promoting agents, anti-inflammatory agents/anti-inflammatory agents/anti-allergic agents, wound healing agents, foaming agents, viscosity-increasing agents, enzymes, purified water (including Electronic water, small clusters, etc.), oral agents, deodorizing/deodorizing agents, bittering agents, seasonings, etc., by combining with these, the cumulative or synergistic improvement of the effects of the present invention or other cosmetic effects can be expected.

Example

The Enterococcus faecalis EC-12, which is the most significant target effect of the present invention, is exemplified as an example of Enterococcus globular lactic acid bacteria, but the present invention is not limited to these examples and the like.

(Deposit of microorganisms)

On February 25, 2005 (the original deposit date), Enterococcus faecalis EC-12 (fecal bowel Coccus EC-12) is deposited at the Patent Bio Deposit Center of the National Institute of Industrial Technology and Technology (Ibaraki Prefecture, Tsukuba City, Ibaraki Prefecture, Higashi 1-chome 1-banchi 1, Central No. 6). The deposit number is FERM BP-10284.

According to a preferred aspect of the present invention, the bacteria used as the active ingredient are bacteria belonging to the genus Enterococcus. More preferably, the bacterium is Enterococcus faecalis.

As Enterococcus faecalis, for example, strains such as Enterococcus faecalis EC-12 (accession number FERM BP-10284), ATCC 19433, ATCC 14508, ATCC 23655, IFO 16803, IFO 16804, and variants thereof can be exemplified. Among the bacteria used as effective ingredients, the above-mentioned EC-12 is the most preferred.

Furthermore, the term "mutant strain" here refers to a specific strain that is mutated by the industry within a range that does not change its properties using methods known to the industry, or that the industry can confirm that it is equivalent By.

(Obtaining of Enterococcus Spherical Lactic Acid Bacteria and Their Heat-treated Dead Bacteria)

Enterococcus faecalis EC-12 (deposit number FERM BP-10284) was purchased from Combi Co., Ltd.

(Manufacture of plant polysaccharides)

Produce true aloe polysaccharides as plant polysaccharides. After crushing 100g of true aloe vera leaves, squeezing, extracting, filtering, adding ethanol or isopropanol to the obtained filtrate and stirring, recovering the precipitate produced, adding ethanol or isopropanol again and washing it , The obtained washings are dried to obtain true aloe polysaccharide powder.

(Sample preparation)

The heat-treated dead cells of Enterococcus spherical lactic acid bacteria produced above were mixed with true aloe polysaccharides to prepare samples 1 to 4 shown in Table 1 (the final concentration of the added medium).

(Cultivation of Staphylococcus epidermidis standard bacteria)

Nutrient Broth No. 2 (Nissui Pharmaceutical Co., Ltd.) was used as the medium. Add samples 1 to 4 to the medium (value in the table: final concentration of the medium %). Then, the standard bacterium Staphylococcus epidermidis (ATCC12228) was inoculated into the medium so as to become 1~5×10 ³ CFU/μL, and the culture was cultured at 37°C for 24 hours. In addition, the control group was assumed to not add heat-treated dead cells of Enterococcus spherical lactic acid bacteria (Sample 1).

(Method of collecting resident bacteria on human skin)

Use a sterile cotton swab to wipe the 3cm square eyebrows 3 times in the vertical direction and 3 times in the horizontal direction to collect the resident bacteria on the skin. Then, the cotton swab was washed in 10 mL of phosphate buffered saline to collect bacteria.

(Cultivation of resident bacteria on human skin)

Nutrient Broth No. 2 (Nissui Pharmaceutical Co., Ltd.) was used as the medium. The enterococcus spheroid lactic acid bacteria were added to the medium to heat-process the dead cells so that the final concentration became 0.25, 0.5, 1.0 w/w%. Then, 100μL of culture medium was inoculated with the skin resident collected by the above method bacteria. These were incubated at 37°C for 24 hours. In addition, the control group was set to not add heat-treated dead cells (0w/w%).

(Determination of the amount of glycerin)

Separate the respective culture solutions of the aforementioned standard Staphylococcus epidermidis bacteria (ATCC12228) and human skin resident bacteria. The culture solution was stirred with a vortex stirrer, and the amount of glycerol was measured using a glycerin detection kit (Cayman Chemical) according to a conventional method.

(Measurement result of glycerin amount)

The results are shown in Figures 1 to 2. By heating the dead cells of Enterococcus spheroid lactic acid bacteria, the production of glycerol derived from the standard bacteria of Staphylococcus epidermidis (ATCC12228) was significantly increased (Figure 1). At the final concentration of 1.0 w/w% (Sample 2) added to the medium, approximately 10 times the glycerol production promoting effect was observed. In addition, in the combination with true aloe polysaccharides (0.1w/w%) (sample 4), the production of glycerol derived from the standard bacteria of Staphylococcus epidermidis synergistically increased. By adding Enterococcus spheroidal lactic acid bacteria to heat the dead bacteria, an increase in glycerol production was also observed in the culture solution of human skin resident bacteria (Figure 2).

(Human observation test)

Male and female sensory inspectors (subjects: 18 in total), after washing their faces before going to bed every day, apply a soft lotion (lotion A) added with enterococcus globular lactic acid bacteria heat-treated dead bacteria to their right cheeks, to their left cheeks Use a placebo soft lotion (lotion B) that has not been added with enterococcus spherical lactic acid bacteria and heat-treated dead cells as a control. Use it for 7 days (the formula is the following soft lotion). After getting up on the 8th day, use it The germicidal cotton swab wipes each cheek of 3 cm square in a manner of 3 times in the vertical direction and 3 times in the horizontal direction to recover the glycerin on the skin surface. Then, Wash the cotton swab in 200μL of phosphate buffered saline to disperse the metabolites.

(Soft lotion)

The above is uniformly mixed according to a conventional method to obtain a soft lotion (lotion A, lotion B).

(Determination of the amount of glycerin)

The above-mentioned dispersion was stirred with a vortex stirrer, and the amount of glycerin was measured by a conventional method using a glycerin detection kit (Cayman Chemical).

(Measurement result of glycerin amount)

The results are shown in Figure 3. When the soft lotion added with enterococcus spherical lactic acid bacteria heat-treated dead cells was used, compared with the soft lotion of the control group, the glycerin on the skin surface of the observation group was significantly increased.

(Human observation test sensory evaluation)

For male and female sensory inspectors (subjects: 18 in total) conducting human observation tests, on the feeling of use of the soft lotion used in the test, and Questionnaire surveys were conducted on the condition of the skin before and after the test. The results of the answers are shown in Table 2.

(Quantitative analysis of human β-defensin-2 (hBD2) and human β-3 (hBD3) mRNA)

In a 24-well culture plate, culture medium for epidermal cells (Humedia KG2, manufactured by Kurabo) was used to culture human epidermal keratinocytes (manufactured by Kurabo) to a subfusion state. Humedia KG2 was further cultured in a differentiation-inducing medium with calcium added to Humedia KG2 in the _{presence of 37°C and 5% CO 2} for 3 days, and then transferred to a new medium. The enterococcus spheroid lactic acid bacteria were added to the medium to heat-process the dead cells so that the final concentration became 0.25, 0.5, 1.0 w/w%. Standard bacteria of Staphylococcus epidermidis were added as a positive target so as to become about 1×10 ^{4 cells/mL.} After 6 hours of culture, RNeasy (Qiagen) was used to extract total RNA from human epidermal keratinocytes. Using each extracted RNA as a template, a reverse transcription reaction was performed using SuperScriptVILO (Invitrogen) by a conventional method to prepare cDNA.

PCR was performed using the above cDNA. The PCR conditions were based on the cDNA sequence of human β-defensin-2 (Accession No. NG_023301) and the cDNA sequence of human β-defensin-3 (Accession No. NC_000008), and the primers shown in Table 3 were prepared. TAKARA Human Housekeeping Gene Primer Set Human ACTB Primer was used as a primer for β-actin used as an internal standard.

Use One Step SYBRR PrimeScript ^TM RT-PCR Kit (TAKARA) to perform PCR reaction, and use TaKaRa PCR Thermal Cycler Dice to perform real-time PCR analysis by conventional methods. According to the above-mentioned real-time PCR analysis, the relative quantification of human β-defensin-2, human β-defensin-3 and β-actin mRNA amount.

(Method for evaluating the amount of human β-defensin-2, human β-defensin-3 and β-actin mRNA)

The human β-defensin-2, human β-defensin-3 and β-actin mRNA amount relative to the internal standard β-actin mRNA amount were evaluated in percentage.

The relative expression level of human β-defensin-2 and human β-defensin-3 mRNA (relative to the control group%) = (human β-defensin-2 and human β-defensin-3 mRNA amount)/(β- Actin mRNA amount)×100

(Quantitative analysis results of human β-defensin-2 and human β-defensin-3 mRNA)

The results are shown in Figure 4. By heating the dead bacteria of Enterococcus spheroid lactic acid bacteria, the expression levels of β-defensin-2 and β-defensin-3 mRNA derived from human epidermal keratinocytes also increased (Figure 4).

(Manufacturing examples of various external preparations for skin protection)

1. Sunscreen cream (O/W cream type)

(Oil phase part)

(Water Phase Department)

(Production method)

Heat the oil phase part and the water phase part to 70°C respectively to dissolve them, fully disperse the titanium dioxide in the water phase part, add the oil phase part, use a homomixer to make the emulsified particles uniform, then perform degassing, filtering, Cool and contain. As the cooling method, it is performed by a method of slowly cooling while stirring while injecting a refrigerant into the jacket of the manufacturing kettle, a method of cooling using a heat exchanger, and the like.

2. Sunscreen lotion (W/O type lotion)

(Oil phase part)

(Water Phase Department)

(Production method)

Heat the oil phase part and the water phase part to 60°C respectively to dissolve them, fully disperse the titanium dioxide and zinc oxide in the oil phase part, add the water phase part, use a homomixer to make the emulsified particles uniform, and then degas , Filtered, cooled and contained.

3. Sunscreen

(Production method)

Add the silicone resin to the silicone oil to dissolve it, then add isopropyl myristate and stir well, then add the remaining ingredients and stir.

4. Powder foundation

(Powder)

Zinc myristate treated talc 20.0%

(Substrate)

(other)

(Production method)

Use a mixer such as a high-speed flow mixer to mix the powder components, and further use a pulverizer to produce fine particles, then add the base material, and use a vertical screw type mixer for thorough mixing. After further crushing and granulating using a hammer mill or the like, compression molding is performed.

5. Dual-use foundation

(Powder)

(Substrate)

(other)

6. Cream (W/O type)

(Phase A)

(Phase B)

(Phase C)

(Phase D)

Phenoxyethanol 0.4%

(Phase E)

Titanium dioxide, twenty oil, aluminum oxide 3.5%

(Phase F)

Dimethyl silylated silicon dioxide 1.0%

(G phase)

(H phase)

Spice 0.3%

(Production method)

Mix the raw materials of phase A under stirring, heat up to 70~75℃, add phase B to phase A while stirring, and stir until uniform. In another container, mix the ingredients of phase C while stirring, heat up to 70~75℃, add phase C to the mixed phase AB, stir while adding slowly (about 5 minutes), add phase C and stir for 3 minutes , And then naturally cool down. When the temperature reaches below 40°C, add phase D and stir for 1 minute, add phase E and stir for 5 minutes, then add phase F and stir for 1 minute, and add phase G and stir for 1 minute. Finally, phase H was added and stirred for 2 minutes, after which it was naturally cooled to room temperature.

7. Moisturizing gel (beauty liquid)

(Phase A)

(Phase B)

(Phase C)

(Production method)

Prepare by mixing phase A and phase B respectively. Disperse the Sanxian gum and sodium polyacrylate by using a batch type disperser, etc., add phase A to phase B at 75°C, homogenize for a few minutes while slowly stirring while cooling, and add C after reaching 40°C Phase (Menthol is pre-dissolved in ethanol).

8.Lipstick

(Phase A)

(Phase B)

(Phase C)

(Phase D)

(Phase E)

(Production method)

Premix phase A evenly, add to phase B and mix evenly, add phase C further, stir slowly while heating to 78~80℃, add phase D and phase E gradually, stir evenly and then flow into the mold , Make it cool naturally to room temperature.

9. Liquid foundation (O/W type)

(Oil phase)

(water box)

(Powder)

(other)

Spices

(Production method)

After the powder is pulverized, the water phase is added and mixed. While stirring, the oil phase and perfume are added to the powder. After being emulsified and dispersed by a homomixer, it is cooled to room temperature.

10. Cream foundation (W/O type)

(Oil phase)

(water box)

(Powder)

(other)

Spices

11. Moisturizing cream (O/W type)

(Phase A)

(Phase B)

(Phase C)

(Production method)

After heating phase A and phase B to 70°C in different containers, add phase A to phase B, homogenize using a batch disperser, etc., and cool to 40°C while stirring. Then, phase C was added to it, further homogenized, and cooled to room temperature.

[Industrial availability]

The enterococcus spherical lactic acid bacteria of the present invention can promote glycerol derived from Staphylococcus epidermidis, which is a skin resident bacteria beneficial to the skin. Production and production of antibacterial peptides derived from skin epidermal keratinocytes. In addition, when applied to the skin, it is dry and has excellent tactility, and it can impart good tactility to any type of external agent of water-based, oil-based, emulsion-based, and powder-based agents. Therefore, it is extremely useful in medicaments for normalizing skin conditions or protecting and maintaining normal skin and external preparations for skin protection.

Claims (3)

A use of dead bacteria of Enterococcus faecalis EC-12 (accession number FERM BP-10284) to produce glycerol production promoter, and the glycerol is derived from Staphylococcus epidermidis. A dead cell of Enterococcus faecalis EC-12 (accession number FERM BP-10284) and the use of true aloe polysaccharides to produce glycerol production promoters, and the glycerol is derived from Staphylococcus epidermidis. A use of dead bacteria of Enterococcus faecalis EC-12 (Accession No. FERM BP-10284) to produce antibacterial peptide production promoters, which are derived from human skin epidermal keratinocytes and are Human β-defensin-2 (hBD2: human β-defensin-2) and/or human β-defensin-3 (hBD3: human β-defensin-3).

Images