CN113499290B - Composition with relieving and repairing effects and preparation method and application thereof - Google Patents
Composition with relieving and repairing effects and preparation method and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q19/005—Preparations for sensitive skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
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Abstract
The invention belongs to the technical field of cosmetics, and discloses a composition with relieving and repairing effects, and a preparation method and application thereof. The composition with the functions of relieving and repairing comprises the following components: peony root bark extract, dendrobium stem extract, polygonum hydropiper extract and matsutake extract. The composition disclosed by the invention effectively exerts a synergistic effect through different action mechanisms of all components, achieves the effects of relieving and repairing, effectively improves sensitive muscle symptoms such as skin redness, dryness and itching, stabbing pain and red swelling, repairs the skin barrier function and enables the skin to recover a healthy state.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composition with soothing and repairing effects, and a preparation method and application thereof.
Background
The healthy skin barrier can help the skin to lock moisture and resist external stimulation. However, due to factors such as air pollution (mainly haze), overtime, living stress, anxiety, poor sun protection, excessive skin care, face-lifting, excessive color makeup, irritant food, electromagnetic radiation and the like, the skin barrier is easily damaged and becomes more and more sensitive, and sensitive problems such as redness, dryness and itching, stabbing pain, red swelling and the like are easily caused.
In addition, in the process of high-speed development of the cosmetic industry, due to the hysteresis of market supervision and the lack of professional identification of consumers, a lot of unqualified and poor-quality cosmetics are abused, some merchants utilize the consumption demand of consumers for quick beauty treatment, and hormone, lead and mercury and other forbidden components are added into products, so that the skin cuticle of the consumers is damaged in many cases. The stratum corneum is damaged, the barrier function of the skin is destroyed, and the skin becomes sensitive skin in a light case and is difficult to cure in a heavy case.
Therefore, how to effectively relieve the sensitive skin problems such as redness, dry itching, stabbing pain, red swelling and the like is a problem to be solved currently.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the composition with the functions of relieving and repairing is provided by the invention, the problems of sensitive skin such as redness, dryness, itching, stabbing pain, redness and swelling can be effectively relieved, the barrier function of the skin is repaired, and the skin is recovered to be in a healthy state.
In a first aspect of the invention, a soothing, repairing composition is provided.
Specifically, the composition with the functions of relieving and repairing comprises the following components: peony root bark extract, dendrobium stem extract, polygonum hydropiper extract and matsutake extract.
Preferably, the composition with the soothing and repairing effects comprises the following components: a peony root-bark aqueous extract, a dendrobium stem aqueous extract, a polygonum hydropiper aqueous extract and a black pine antler alcohol extract.
The composition with the functions of relieving and repairing has the following effects:
the peony root bark, originally recorded in Shen nong Ben Cao Jing (Shen nong's herbal), is bitter, pungent and slightly cold in flavor, and enters heart, liver and kidney meridians, and has the effects of clearing heat and cooling blood, promoting blood circulation and removing blood stasis. The paeonol-containing compound is characterized by comprising paeonol, and the paeonol-containing compound is rich in polyphenols, flavonoids, polysaccharides, amino acids, trace elements and other substances, and has multiple effects of good bacteriostasis, inflammation resistance, detumescence, oxidation resistance, moisture retention and the like. The peony root bark aqueous extract of the invention is as follows: adopting dry peony root bark, wherein the ratio of material to liquid is 1: 10-20, adding water, heating, distilling under reduced pressure, collecting the distillate for the first 1-3h, adding a proper amount of preservative, sterilizing, standing, and filtering to obtain the peony root bark aqueous extract. Preferably: adopting dry peony root bark, wherein the ratio of material to liquid is 1: 15 adding water, heating, distilling under reduced pressure, collecting the distillate for the first 2h, adding a proper amount of preservative, sterilizing, standing, and filtering to obtain the peony root-bark aqueous extract.
Dendrobium nobile is a traditional famous and precious traditional Chinese medicine in China, and is listed as the top grade in the Shen nong's herbal Jing. Modern medicine and traditional Chinese medicine pharmacological research show that the dendrobium has obvious effects on improving human body immunity, resisting aging, inhibiting tumors, tonifying internal organs and consumptive disease and the like. The dendrobium stem aqueous extract contains rich dendrobium polysaccharide, and has the effects of promoting the expression of skin aquaporin APQ-3 and strongly moisturizing. The stem aqueous extract of dendrobium nobile of the invention refers to: drying the stem of dendrobium nobile lindl, crushing to 100-mesh and 200-mesh, wherein the material-liquid ratio is 1: 10-20 adding water, reflux-extracting at 80-90 deg.C for 1-3 times, centrifuging to remove residue, and concentrating the extractive solution until the weight ratio of the extractive solution to the medicinal materials is 1: (1-3) adding ethanol with the weight 2-3 times of that of the concentrated solution into an alcohol precipitation tank each time for 1-2h, uniformly stirring, standing overnight, centrifugally separating, collecting precipitate, vacuum drying at 55-65 ℃, adding hot water and a proper amount of preservative into the obtained dry powder, and preparing the dendrobium stem aqueous extract. Preferably: adopting dry dendrobium stem, crushing to 150 meshes, wherein the material-liquid ratio is 1: 15, adding water, reflux-extracting at 85 ℃ for 2 times, centrifuging to remove dregs, and concentrating the extracting solution until the weight ratio of the extracting solution to the medicinal materials is 1: 2, adding ethanol with the weight 3 times that of the concentrated solution into an alcohol precipitation tank for 1.5h each time, uniformly stirring, standing overnight, centrifugally separating and collecting precipitates, carrying out vacuum drying at 60 ℃, adding hot water and a proper amount of preservative into the obtained dry powder, and preparing the dendrobium stem aqueous extract.
The herba Polygoni Hydropiperis is dry whole plant of herba Polygoni Hydropiperis (Polygonaceae) of Polygonum, also called herba Polygoni Hydropiperis, and herba Polygoni Hydropiperis, has pungent taste, acid and warm property, has effects of dispelling pathogenic wind and promoting diuresis, removing toxic substance and detumescence, removing blood stasis and relieving pain, and relieving itching, and mainly contains flavone, sesquiterpene and phenolic acid components. Pharmacological research shows that the polygonum hydropiper has broad-spectrum antibacterial, anti-inflammatory, antioxidant and other effects. The application in cosmetics is mainly used for relieving, resisting inflammation and the like. The polygonum hydropiper aqueous extract of the invention is characterized in that: adopting dry polygonum hydropiper, wherein the ratio of material to liquid is 1: 15 adding water, heating, distilling under reduced pressure, collecting the distillate for the first 2h, adding appropriate amount of antiseptic, sterilizing, standing, and filtering to obtain herba Polygoni Hydropiperis aqueous extract. Preferably: adopting dry polygonum hydropiper, wherein the ratio of material to liquid is 1: adding water 10-20, heating, distilling under reduced pressure, collecting distillate 1-3 hr earlier, adding appropriate amount of antiseptic, sterilizing, standing, and filtering to obtain herba Polygoni Hydropiperis water extract.
The tricholoma matsutake, also called tricholoma matsutake, is a pure natural rare and precious wild edible fungus, contains rich amino acids, polysaccharides, proteins, various vitamins and other components, and has very good effects of resisting free radicals, protecting cells from ultraviolet damage, resisting oxidation and aging and the like. The black pine antler alcohol extract of the invention refers to: adopting dry black pine mushroom, wherein the ratio of material to liquid is 1: (5-15) adding 75-80% ethanol solution, extracting for 1-3h at 70-85 deg.C under heating in water bath, extracting for 2-3 times, filtering, concentrating, and diluting to obtain ethanol extract of Pinus nigra (Thunb.) Var. Preferably: adding 75% ethanol solution into dried black pine mushroom at a ratio of 1:10, extracting for 2 times at 80 deg.C under heating in water bath for 2 hr, filtering, concentrating, and diluting to obtain black pine mushroom ethanol extract.
The composition with the effects of relieving and repairing is prepared by scientifically proportioning the components of a peony root bark water extract, a dendrobium stem water extract, a polygonum hydropiper water extract and a matsutake alcohol extract, wherein the peony root bark water extract has the effects of relieving swelling and resisting inflammation, the dendrobium stem water extract replenishes water and preserves moisture, the polygonum hydropiper water extract has the effects of relieving and resisting allergy, the matsutake water extract clears free radicals, and the skin barrier damage caused by free radical damage is reduced; through multiple approaches of anti-allergy repair, water replenishing and moisture retention, free radical removal, skin barrier strengthening and the like, the components are synergistic and supplement each other, so that the effects of relieving and repairing the skin barrier function are achieved.
Preferably, the composition with the functions of relieving and repairing comprises the following components in percentage by mass: 0.001-25% of peony root-bark aqueous extract, 0.001-25% of dendrobium stem aqueous extract, 0.01-15% of polygonum hydropiper aqueous extract, 0.001-15% of auricularia julibrissin alcohol extract and 20-99% of water.
Preferably, the composition with the functions of relieving and repairing comprises the following components in percentage by mass: 5-25% of peony root bark aqueous extract, 5-25% of dendrobium stem aqueous extract, 0.01-5% of polygonum hydropiper aqueous extract, 0.01-5% of auricularia auriculata alcohol extract and the balance of water 40-89%.
Preferably, the composition with the functions of relieving and repairing comprises the following components in percentage by mass: 5-15% of peony root bark aqueous extract, 5-15% of dendrobium stem aqueous extract, 0.01-2% of polygonum hydropiper aqueous extract, 0.1-2% of matsutake alcohol extract and 66-89% of water.
Preferably, the composition with the functions of relieving and repairing comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of polygonum hydropiper aqueous extract, 15% of matsutake alcohol extract and 20% of water.
In a second aspect, the invention provides a method for preparing the composition with soothing and repairing effects.
Specifically, the preparation method of the composition with the functions of relieving and repairing comprises the following steps: mixing the components to obtain the composition with the functions of relieving and repairing.
According to a third aspect of the invention, there is provided the use of said soothing, repairing composition in cosmetics.
Preferably, the skin cream comprises the composition with the soothing and repairing effects and auxiliary materials.
Preferably, the composition with the soothing and repairing effects accounts for 0.01-30% of the skin cream by mass.
Preferably, the composition with the soothing and repairing effects accounts for 0.01-2% of the skin cream by mass.
Preferably, the auxiliary material comprises at least one of a humectant, an antioxidant, an emollient and a preservative.
Compared with the prior art, the invention has the following beneficial effects:
the composition with the functions of relieving and repairing is prepared by compounding the peony root bark extract, the dendrobium stem extract, the polygonum hydropiper extract and the black pine mushroom extract, effectively exerts a synergistic effect through different action mechanisms of all components, achieves the effects of relieving and repairing, effectively improves sensitive muscle symptoms such as skin redness, dry itching, stabbing pain, redness and swelling, repairs the skin barrier function and enables the skin to recover the healthy state.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
The preparation methods of the extracts in the following examples are respectively as follows:
the peony root bark aqueous extract: adopting dry peony root bark, wherein the ratio of material to liquid is 1: 10-20, adding water, heating, distilling under reduced pressure, collecting the distillate for the first 1-3h, adding a proper amount of preservative, sterilizing, standing, and filtering to obtain the peony root-bark aqueous extract.
Dendrobium stem aqueous extract: drying the stem of dendrobium nobile lindl, crushing to 100-mesh and 200-mesh, wherein the material-liquid ratio is 1: 10-20, adding water, reflux-extracting at 80-90 deg.C for 1-3 times, centrifuging to remove residue, and concentrating the extractive solution until the weight ratio of the extractive solution to the medicinal materials is 1: (1-3) adding ethanol with the weight 2-3 times of that of the concentrated solution into an alcohol precipitation tank each time for 1-2h, uniformly stirring, standing overnight, centrifugally separating, collecting precipitate, vacuum drying at 55-65 ℃, adding hot water and a proper amount of preservative into the obtained dry powder, and preparing the dendrobium stem aqueous extract.
The polygonum hydropiper aqueous extract refers to: adopting dry polygonum hydropiper, wherein the ratio of material to liquid is 1: 15 adding water, heating, distilling under reduced pressure, collecting the distillate for the first 2h, adding appropriate amount of antiseptic, sterilizing, standing, and filtering to obtain herba Polygoni Hydropiperis aqueous extract.
Extracting the alcohol extract of the black pine antler: adopting dry black pine mushroom, wherein the ratio of material to liquid is 1: (5-15) adding 75-80% ethanol solution, extracting for 1-3h at 70-85 deg.C under heating in water bath, extracting for 2-3 times, filtering, concentrating, and diluting to obtain ethanol extract of Pinus nigra (Thunb.) Var.
Example 1
A composition with soothing and repairing effects comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of polygonum hydropiper aqueous extract, 15% of matsutake alcohol extract and 20% of water.
The preparation method of the composition with the functions of relieving and repairing comprises the following steps: mixing the above components to obtain the composition with soothing and repairing effects.
Example 2
A composition with soothing and repairing effects comprises the following components in percentage by mass: 15% of peony root bark aqueous extract, 15% of dendrobium stem aqueous extract, 10% of polygonum hydropiper aqueous extract, 10% of matsutake alcohol extract and 50% of water.
The preparation method of the composition with the functions of relieving and repairing comprises the following steps: mixing the above components to obtain the composition with soothing and repairing effects.
Example 3
A composition with soothing and repairing effects comprises the following components in percentage by mass: 5% of peony root bark aqueous extract, 5% of dendrobium stem aqueous extract, 5% of polygonum hydropiper aqueous extract, 5% of matsutake alcohol extract and 80% of water.
The preparation method of the composition with the functions of relieving and repairing comprises the following steps: mixing the above components to obtain the composition with soothing and repairing effects.
Comparative example 1
An aqueous extract of peony root bark was used as comparative example 1.
Comparative example 2
The aqueous extract of stem of dendrobium nobile lindl was used as comparative example 2.
Comparative example 3
The polygonum hydropiper aqueous extract was used as comparative example 3.
Comparative example 4
The alcohol extract of matsutake was used as comparative example 4.
Comparative example 5 (different from example 1 in that it does not contain the alcohol extract of matsutake)
The composition comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of polygonum hydropiper aqueous extract and 35% of water. The preparation method is the same as that of example 1.
Comparative example 6 (different from example 1 in that no aqueous Polygonum hydropiper extract was contained)
The composition comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of auricularia auricula-judae ethanol extract and 35% of water. The preparation method is the same as example 1.
Comparative example 7 (different from example 1 in that it does not contain the stem aqueous extract of Dendrobium nobile Lindl.)
The composition comprises the following components in percentage by mass: 25% of peony root-bark aqueous extract, 15% of polygonum hydropiper aqueous extract, 15% of matsutake alcohol extract and 45% of water. The preparation method is the same as that of example 1.
Comparative example 8 (different from example 1 in that aqueous extract of peony root bark is not contained)
The composition comprises the following components in percentage by mass: 25% of dendrobium stem water extract, 15% of polygonum hydropiper water extract, 15% of matsutake alcohol extract and 45% of water. The preparation method is the same as that of example 1.
Comparative example 9 (different from example 1 in that aqueous extract of peony root bark was replaced with aqueous extract of centella asiatica having similar effects)
The composition comprises the following components in percentage by mass: 25% of centella aqueous extract, 25% of dendrobium stem aqueous extract, 15% of polygonum hydropiper aqueous extract, 15% of matsutake alcohol extract and 20% of water. The preparation method is the same as that of example 1.
Comparative example 10 (different from example 1 in that the aqueous extract of stem of Dendrobium nobile was replaced with an aqueous extract of Viola tricolor having similar effects)
The composition comprises the following components in percentage by mass: 25% of a peony root-bark aqueous extract, 25% of a pansy aqueous extract, 15% of a polygonum hydropiper aqueous extract, 15% of a matsutake alcohol extract and 20% of water. The preparation method is the same as example 1.
Comparative example 11 (different from example 1 in that the aqueous extract of polygonum hydropiper was replaced with an aqueous extract of purslane having similar effects)
The composition comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of purslane aqueous extract, 15% of auricularia nigra ethanol extract and 20% of water. The preparation method is the same as that of example 1.
Comparative example 12 (different from example 1 in that the alcohol extract of matsutake was replaced with alcohol extract of ginseng having similar effects)
The composition comprises the following components in percentage by mass: 25% of peony root bark aqueous extract, 25% of dendrobium stem aqueous extract, 15% of polygonum hydropiper aqueous extract, 15% of ginseng alcohol extract and 20% of water. The preparation method is the same as that of example 1.
Composition Effect test
Experimental example 1 in vitro hyaluronidase inhibition experiment
Hyaluronidase is a participant of type I anaphylaxis, and hyaluronidase has strong correlation with inflammation and allergy, so the in vitro hyaluronidase activity inhibition experiment can be used as an index for researching the specific action of an anti-allergy component.
The inhibition of hyaluronidase activity was determined by the Elson-Morgan (N-methylglucamine reaction) modification. Examples 1 to 3 and comparative examples 1 to 12 were prepared as sample solutions having a concentration of 1mg/mL, respectively, to be used. 0.1mL of CaCl with the concentration of 0.25mmol/L is taken2Adding 0.5mL hyaluronidase solution (1250U/mL), and keeping the temperature at 37 ℃ for 20 min; adding 0.5mL of sample solution, and keeping the temperature at 37 ℃ for 20 min; adding 0.5mL of sodium hyaluronate solution with concentration of 0.5g/L, preserving heat at 37 ℃ for 30min, and then standing at normal temperature for 5 min; then adding 0.1mL NaOH solution with the concentration of 0.4mol/L and 0.5mL acetylacetone solution, placing in a boiling water bath, heating for 15min, and immediately cooling with cold water for 5 min; adding 1mL of Ellisib reagent, diluting with 3mL of anhydrous ethanol, mixing, and standing for 20min for color development. And (3) taking dipotassium glycyrrhizinate as a positive control, and measuring a light absorption value by using an ultraviolet visible spectrophotometer.
Wherein, A: absorbance of control solution (the sample solution was replaced with acetate buffer); b: comparing the light absorption value of the blank solution (replacing the sample solution and the enzyme solution with acetic acid buffer solution); c: absorbance of sample solution, D: and (4) the light absorption value of a blank sample solution (the enzyme solution is replaced by an acetic acid buffer solution). The A solution was first scanned over a range of 450nm to 700nm to determine the maximum absorption wavelength. The results of the hyaluronidase inhibition experiments are shown in table 1.
TABLE 1 Hyaluronidase inhibition results
| Sample (I) | Hyaluronidase inhibition rate |
| Example 1 | 90.63％ |
| Example 2 | 79.25％ |
| Example 3 | 67.58％ |
| Comparative example 1 | 47.58％ |
| Comparative example 2 | 32.05％ |
| Comparative example 3 | 21.81％ |
| Comparative example 4 | 15.25％ |
| Comparative example 5 | 60.23％ |
| Comparative example 6 | 51.64％ |
| Comparative example 7 | 40.71％ |
| Comparative example 8 | 22.05％ |
| Comparative example 9 | 45.69％ |
| Comparative example 10 | 51.38％ |
| Comparative example 11 | 48.94％ |
| Comparative example 12 | 36.73％ |
As can be seen from Table 1, the composition with soothing and repairing effects of the invention has better hyaluronidase inhibition capability, and the four components have synergistic effect. From the experimental data, the compositions of examples 1-3 all had significantly greater hyaluronidase inhibition than comparative examples 1-4, i.e., the effect of the individual components. In the experimental process, the hyaluronidase inhibition effect of the alcohol extract of the matsutake is found to be weak, only 15.25%, but the hyaluronidase inhibition rate of the whole composition can be obviously improved after the alcohol extract of the matsutake and the other three components are compounded, which shows that a synergistic relationship exists among the four components of the composition, and if one component is lacked, the effect is greatly reduced. In addition, as can be seen from the hyaluronidase inhibition experiment results of comparative examples 9-12, even if any one component in the composition of the present invention is replaced by other components with similar efficacy, the hyaluronidase inhibition rate is much lower than that of the compositions of examples 1-3, further showing that the peony root bark aqueous extract, the dendrobium stem aqueous extract, the polygonum hydropiper aqueous extract and the auricularia thunbergii ethanol extract in the composition of the present invention have a synergistic relationship, but none of them is acceptable. The hyaluronidase inhibition ability of the composition of the invention is the same as that of the embodiment by replacing the aqueous extract with the alcoholic extract or replacing the alcoholic extract with the aqueous extract.
Experimental example 2 evaluation of moisturizing and repairing effects on human skin
An acute skin barrier injury model was established by a tape continuous sticking method, and changes in the TWEL value of the percutaneous water loss amount and the EI value of the skin erythema index before and after the use of the sample were measured by a skin moisture tester (CK, Germany).
Firstly, a subject: 10 healthy subjects aged 19-36 years, averaged (26.70 + -4.64 years), mainly indoors, without a great history of exposure to sunlight and a history of non-allergic diseases; the measurement site is free of skin disorders, pigmentation, scarring or systemic diseases that may affect the skin; except for those who use anti-allergic drugs, corticosteroids and other drugs affecting skin metabolism within 3 months. All subjects were informed of the course of the experiment and signed informed consent.
Measurement conditions: the method is carried out under the conditions of constant temperature and constant humidity, the temperature is (22 +/-1) DEG C, the humidity is (41.5 +/-0.5)%, a tested person enters a testing environment for quiet testing 30min in advance, the testing part is exposed 10min before the testing, and each parameter is continuously measured for 3 times to take an average value so as to reduce the measurement error.
Experimental materials and equipment: the skin physiological noninvasive detection devices are from CK corporation of Germany, Corneofix adhesive tape, a percutaneous moisture loss measuring instrument (TewameterRTM300), a horny layer moisture content measuring instrument (CorneometerRCM825), an erythema index measuring instrument (MexametrMX 18) and the like.
Fourthly, the test method: selecting a piece of skin with the size of 2cm multiplied by 2cm on the back of a subject as a test area and marking the test area, and measuring various physiological and physical index baseline values related to the skin barrier function. Measurement indexes are as follows: TWEL value of transdermal water loss, Erythema Index (EI). Taking the compositions obtained in examples 1-3 and comparative examples 1-12 as test samples, establishing an acute skin barrier injury model by using a tape continuous sticking method, and randomly dividing two test areas into a natural repair area and a sample test area; the sample test area is smeared with 2mg of test samples in the morning and at night every day, and the natural repair area is used as a blank control without other treatment so as to observe the natural repair process of the skin barrier; measuring various indexes before smearing the sample every morning; the samples of each composition were compared for post-skin injury repair. The results are shown in tables 2 and 3 (D0 represents day 0, D3 represents 3 days after use, and D7 represents 7 days after use).
TABLE 2 Change in the TEWL mean values of the subjects' transdermal Water loss rates over the 7 days
TABLE 3 Change in skin erythema index EI values of subjects using 7 days
The results of the experiments in tables 2 and 3 show that the compositions of examples 1 to 3 have good skin barrier soothing and repairing functions.
After the adhesive tape is continuously adhered, the skin barrier of a subject is damaged, the transdermal water loss value is greatly improved, meanwhile, the skin generates certain inflammation and red swelling reaction, and the erythema index of the skin continuously rises within a period of time. Compared with the blank control and the comparative examples 1 to 4, the skin barrier function of the subjects of the examples 1 to 3 is greatly improved after 3 days of use, the transdermal water loss value is greatly reduced, and the erythema index EI is effectively controlled. After 7 days of use, the transdermal water loss value of the subject is lower than that before the experiment, and the erythema index EI value also reaches or is lower than that before the experiment, which shows that the skin inflammation and the erythema reaction of the subject are effectively relieved, the barrier function is recovered, even better than the original value, and compared with the prior art, the soothing and repairing effect of a single component is not obvious. From the data of the test on the value of water loss through skin of comparative examples 5 to 8 and comparative examples 9 to 12, the composition lacking any component, or replacing a component in the composition of the present invention with a component having similar efficacy, has much poorer effect of soothing and repairing than the compositions of examples 1 to 3, which shows that the aqueous extract of peony root bark, the aqueous extract of dendrobium stem, the aqueous extract of polygonum hydropiper and the alcoholic extract of matsutake in the composition of the present invention have synergistic effect, and the four components are none at all. The water extract in the composition is replaced by the alcohol extract, or the alcohol extract is replaced by the water extract, and the composition still has the same functions of relieving and repairing skin barriers as the embodiment.
Experimental example 3 measurement of superoxide anion radical scavenging ability
Taking 4.5mL of Tris-HCl buffer solution with the pH value of 8.2 and the concentration of 0.05mol/L, preheating the solution in a water bath kettle at 25 ℃ for 20min, respectively adding 1mL of the compositions of examples 1-3 and comparative examples 1-12, then adding 0.4mL of pyrogallol solution with the concentration of 25mmol/L, uniformly mixing, reacting in a water bath at 25 ℃ for 5min, and adding 1.0mL of HCl with the concentration of 8mol/L to terminate the reaction.
Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. The blank was replaced with 1mL of distilled water and the superoxide anion radical scavenging rate (D) was calculated as follows.
D(％)＝[1-(A2/A1)]×100％；
In the formula A1Absorbance values for the blank; a. the2Is the absorbance value of the sample. The results of the experiment are shown in table 4.
Experimental example 4 measurement of hydroxyl radical scavenging ability
3mL of FeSO with the concentration of 2mmol/L are sequentially added into a 25mL colorimetric tube43mL of H with a concentration of 1mmol/L2O2Shaking, adding salicylic acid 3mL with concentration of 6mmol/L, shaking, heating in 37 deg.C water bath for 15min, and measuring absorbance A0. Then 100 mg. L are added-1The compositions of examples 1 to 3 and comparative examples 1 to 12 were shaken up, heated in a water bath for 15min, taken out and measured for absorbance Ax. According to the method, another colorimetric tube is taken, the above liquids are repeatedly and sequentially added, no salicylic acid is added, the mixture is shaken up and heated in water bath for 15min, and the value A is measuredx0. The hydroxyl radical scavenging rate (D) was calculated as follows.
D(％)＝A0-(Ax-Ax0)/A0×100％；
In the formula A0The absorbance value of the blank control system is obtained; a. thexAdding the absorbance value of a sample system to be detected; a. thex0Without adding a color-developing agent H2O2Absorbance value of solution background. The results of the experiment are shown in table 4.
TABLE 4 clearance of two free radicals
As can be seen from Table 1, the compositions obtained in examples 1-3 all have good radical scavenging ability, with the highest scavenging rate of superoxide anion radicals being 85.18%, and the highest scavenging rate of hydroxyl radicals being 79.61%. The radical scavenging effect of the compositions obtained in examples 1-3 was much stronger than that of comparative examples 1-4 using the single component alone, and also much stronger than that of comparative examples 5-8 combining any three components together. The compositions obtained in comparative examples 9 to 12, which have weaker radical scavenging ability than the compositions of examples 1 to 3 in view of experimental results due to replacement of a component in the composition of the present invention with a component having similar efficacy, have better radical scavenging efficacy than the compositions of examples 1 to 3 in which comparative example 12 replaces the alcohol extract of matsutake alcohol in the composition of the present invention with a ginseng alcohol extract recognized to have better radical scavenging effect, but the results are still inferior to the compositions of examples 1 to 3 in the present invention. Therefore, the peony root-bark aqueous extract, the dendrobium stem aqueous extract, the polygonum hydropiper aqueous extract and the auricularia auricula ethanol extract in the composition have a synergistic effect, and one of the four components is not necessary. The water extract in the composition is replaced by the alcohol extract, or the alcohol extract is replaced by the water extract, and the composition still has the radical scavenging capacity which is equal to that of the embodiment.
Application example 1
The composition obtained in example 1 of the present invention was used to prepare a skin cream having the formulation components shown in table 5.
TABLE 5 skin cream formulations
The skin cream is prepared according to a conventional preparation method.
Comparative application example 1 (differing from application example 1 only in that the composition of example 1 was not contained, and other components and contents and preparation method were kept unchanged).
Experimental example 5 evaluation of moisturizing and repairing effects on human skin
An acute skin barrier injury model was established by a tape continuous sticking method, and changes in the TWEL value of the percutaneous water loss before and after the use of the sample of application example 1 and the comparative application example 1 and the EI value of the skin erythema index were measured by a skin moisture tester (CK, Germany). The results are shown in tables 6 and 7.
The subject: 10 healthy subjects aged 19-36 years, averaged (26.70 ± 4.64) years, mainly indoors, without a substantial history of exposure to sunlight and a history of non-allergic diseases; the measurement site is free of skin disorders, pigmentation, scarring or systemic diseases that may affect the skin; except for those who used anti-allergic drugs, corticosteroids, etc. affecting skin metabolism within 3 months. All subjects were informed of the experimental process and signed informed consent.
Measurement conditions are as follows: the method is carried out under the conditions of constant temperature and constant humidity, the temperature is (22 +/-1) DEG C, the humidity is (41.5 +/-0.5)%, a tested person enters a testing environment for quiet testing 30min in advance, the testing part is exposed 10min before the testing, and each parameter is continuously measured for 3 times to take an average value so as to reduce the measurement error.
Experimental materials and equipment: the skin physiological noninvasive detection devices are from CK company of Germany, Corneofix R adhesive tape, a percutaneous moisture loss measuring instrument (TewameterTM 300), a horny layer moisture content measuring instrument (CorneometerRCM825), an erythema index measuring instrument and the like.
Fourthly, the test method: selecting a piece of skin with the size of 2cm multiplied by 2cm on the back of a subject as a test area and marking the test area, and measuring various physiological and physical index baseline values related to the skin barrier function. Measurement indexes are as follows: the transdermal water loss TWEL value, the Erythema Index (EI); establishing an acute skin barrier damage model by using a tape continuous sticking method, randomly dividing two test areas into a natural repair area and a sample test area, smearing 2mg of test samples in the sample test area in the morning and at the evening of each day, and using the natural repair area as a blank control without other treatment to observe the natural repair process of the skin barrier; measuring various indexes before smearing the sample every morning; the samples of each composition were compared for post-skin injury repair. (in the table, D0 represents day 0, D3 represents 3 days after use, and D7 represents 7 days after use)
TABLE 6 Change in TEWL mean values of subjects' transdermal Water loss for 7 days
TABLE 7 variation of skin erythema index EI values in subjects with 7 days
In combination with the experimental data in tables 6 and 7, compared with comparative application example 1, the skin transdermal water loss rate and the erythema index of the subject are obviously improved and basically restored to the levels before the experiment after the subject uses the sample in application example 1 for 7 days, while the skin barrier repair condition of the subject is slower and is slightly better than that of a natural repair area by using the sample in comparative application example 1, which shows that the composition provided by the invention is added into cosmetics, so that the cosmetics have corresponding mild repair effect.
Claims (10)
1. A composition comprising the following components: water extracts of peony root bark, dendrobium stem, polygonum hydropiper and alcohol extracts of matsutake.
2. The composition according to claim 1, which comprises the following components in percentage by mass: 0.001-25% of peony root bark water extract, 0.001-25% of dendrobium stem water extract, 0.01-15% of polygonum hydropiper water extract, 0.001-15% of matsutake alcohol extract and 20-99% of water.
3. The composition according to claim 2, which comprises the following components in percentage by mass: 5-25% of peony root bark water extract, 5-25% of dendrobium stem water extract, 0.01-5% of polygonum hydropiper water extract, 0.01-5% of auricularia nigra ethanol extract and 40-89% of water.
4. The composition according to claim 2, which comprises the following components in percentage by mass: 5-15% of a peony root bark water extract, 5-15% of a dendrobium stem water extract, 0.01-2% of a polygonum hydropiper water extract, 0.1-2% of an auricularia nigra linn alcohol extract and 66-89% of water.
5. A process for the preparation of a composition according to any one of claims 1 to 4, characterized in that it comprises the following steps: mixing the components to obtain the composition.
6. Use of a composition according to any one of claims 1 to 4 for the preparation of a cosmetic product.
7. A skin cream characterized by comprising the composition of any one of claims 1 to 4 and an adjuvant.
8. The skin cream of claim 7, wherein the composition is present in an amount of 0.01-30% by weight of the cream.
9. The skin cream of claim 8, wherein the composition is present in an amount of 0.01-2% by weight of the cream.
10. The skin cream of claim 7, wherein the adjuvant comprises at least one of a moisturizer, an antioxidant, an emollient, and a preservative.
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| PCT/CN2021/132448 WO2023284221A1 (en) | 2021-07-15 | 2021-11-23 | Composition with soothing and repairing effects, and preparation method therefor and use thereof |
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| CN113499290B (en) * | 2021-07-15 | 2022-06-17 | 广州环亚化妆品科技股份有限公司 | Composition with relieving and repairing effects and preparation method and application thereof |
| CN114028296A (en) * | 2021-12-13 | 2022-02-11 | 上海宜侬生物科技有限公司 | Multi-dimensional skin sensitivity repairing cosmetic component and application |
| CN115120543B (en) * | 2022-07-21 | 2023-11-10 | 菏泽市亿鑫生物科技有限公司 | Sophora japonica and saccharomycete fermentation liquor allergy-relieving composition and preparation and application thereof |
| CN115487266B (en) * | 2022-08-25 | 2023-09-01 | 无限极（中国）有限公司 | Composition with endothelin inhibition effect and preparation method and application thereof |
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| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN108969447B (en) * | 2018-07-19 | 2021-10-01 | 北京润熙怡和科技有限公司 | Skin care composition for repairing damaged cells and sensitive skin and preparation method thereof |
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| CN113499290B (en) * | 2021-07-15 | 2022-06-17 | 广州环亚化妆品科技股份有限公司 | Composition with relieving and repairing effects and preparation method and application thereof |
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Address after: 510663 No.15, Kelin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Applicant after: Guangzhou Huanya cosmetics technology Co.,Ltd. Address before: 510000 No. 15, Colin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangzhou, Guangdong Applicant before: GUANGZHOU HUANYA COSMETICS TECHNOLOGY Co.,Ltd. |
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