CN109453098A

CN109453098A - One kind is releived composition and its preparation method and application

Info

Publication number: CN109453098A
Application number: CN201811583295.4A
Authority: CN
Inventors: 吴知情; 刘薇; 胡根华; 胡兴国; 胡根润; 陈庆生; 杨雨琪
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2018-12-24
Filing date: 2018-12-24
Publication date: 2019-03-12
Anticipated expiration: 2038-12-24
Also published as: CN109453098B

Abstract

It releives composition and its preparation method and application the present invention provides one kind, the composition of releiving includes following component by weight percent: Aloe Barbadensis Miller water 10-50%, cyani flos water 10-30%, Herba polygoni hydropiperis extract 1-7% and ginger-root extract 1-5%；The mass percent of each component is not more than 100%.Composition of the present invention of releiving is made of the natural plant composition of plurality of stable, it is highly-safe, skin irritation symptom is effectively relieved by blocking allergic reaction generation or development, enhancing body barrier function and removing the multiple pathways such as surplus free radical in vivo, promotes skin immunity.

Description

One kind is releived composition and its preparation method and application

Technical field

The invention belongs to cosmetic field, it is related to a kind of composition and its preparation method and application, relates generally to a kind of relax Slow composition and its preparation method and application, and in particular to a kind of composition with repair of releiving and preparation method thereof and Using.

Background technique

In recent years, with the increase of the variation of dietary structure, environmental pollution and job and life stress, allergic human population sharply increases Add, especially in coastal developed regions, skin sensitivity has become the social concern for influencing human health.With sensibility skin disease The crowd of shape is existing just to there is the trend dramatically increased, especially in male population.More and more crowds think that oneself has Sensibility skin；Such as in Europe, the U.S. and Japan, about 50% women and Yue 30% male think it oneself is sensibility flesh Skin, and in China, this ratio reaches about 36%.It can be seen that skin sensitivity becomes the potential trend of globalization, it is consumer The skin problem of concern.

Actually and non-disease, it is to instigate skin to the physiology overreaction of environment or internal factor to sensibility skin State (Enzo Berardesca, Italy).Contact skin care item, soap, sunscreen product, detergent are likely to this shape occur State, and can also aggravate common skin malaise symptoms in dry and cold environment.Cause skin allergy or damage because being known as Very much: physical factor has ultraviolet light, heat, cold, mechanical damage etc.；Chemical factor have the excessive degreasing of surfactant, chemical solvent, Other harmful chemicals such as sun-screening agent etc.；Biological factor has bacterium, mould, germ etc. that can all cause skin allergy or damage. These factors cause the skin injury occurred to have the oxidative damage of free radical, the damage of cell DNA, inflammation damnification, ultraviolet light to draw The photoaging damage that rises, breaks down proteins and wrinkle of skin caused by being crosslinked, excessively washing cause skin barrier impaired, immune thin Cellular damage, dry skin etc..

There are mainly three types of common skin injuries: ultraviolet injury, inflammation damnification and skin barrier damage.1. ultraviolet line loss Wound is to cause inflammation because ultraviolet light can excite free radical, leads to erythema and dry skin；Activate MMP (metalloprotein Enzyme) cause protein-crosslinking, damaging cells DNA damages skin immune system；Activate tyrosinase, cause skin splash with it is tanned Deng.2. various physics (such as air pollution, mechanical damage, temperature change), (such as detergent, bleaching agent, has decorticating agent chemistry Solvent etc.), the extraneous factors such as biological (such as bacterium, virus, mycoplasma, mould) stimulate skin, lead to the reduction of its tolerance, To induce inflammation, there are the symptoms such as flush, fever, oedema, and generates neural sensation stimulation such as burning sensation, tingling sensation, scabies Itching and pain.3. the barrier function of skin is mainly related with the special construction of cuticula.Intercellular lipid is tight keratinocyte It is close to combine, the lost barrier of prevention and treatment moisture of skin is formed, while skin also being protected not injured by extraneous harmful substance, and one The factors such as a little external force damages, skin aging, chemical stimulation, dry weather will cause the weakening of skin barrier, to aggravate to pierce The transdermal penetration for swashing object, further results in skin damage.

In general, the mechanism that scytitis reaction occurs is skin by extraneous factor (machinery, physics, chemistry etc.) It influences to cause the loss of the water soluble ingredient NMF, lipid and albumen of intercellular lipid bilayer so that skin barrier is impaired, A large amount of level-one inflammatory factors (such as tumor necrosis factor TNF-alpha, interleukin I L1- α), this kind of cytotropism can be released simultaneously Inflammatory factor is combined with intracorporal mast cell, keratinocyte, and cascade reaction occurs, further discharges proinflammatory mediators IL-6 With IL-8 and the secondary inflammation factor (such as Cycloxygenase COX-2, Prostaglandin PGE₂Deng), it aggravates cascade reaction and expands blood vessel, Gargalesthesia medium and overactivity thermo receptor are discharged simultaneously, enables sensory nerve more sensitive, generates different degrees of gargalesthesia, calcination Sense, tingling sensation even erythema and fash.

Cosmetic field mainly has two developing directions in recent years: first is that pursuing natural plant, making every effort in cosmetics Use natural plants Effect raw material；Second is that the effect of consumer is to cosmetics are pursued further.In this context, for Shu Min class On the one hand cosmetic, most of brands are dedicated to finding the plant resources of zero addition and zero burden as cosmetic material to reduce pair The irritation of skin；On the other hand active material is found from plant to be repaired skin barrier function to enhance cosmetics and resisting The effect of property in terms of scorching Shu Min.Certainly, the application of plant must be set up on the system of scientific application, not only need further Reinforce also reinforcing to efficacy assessments and security performance the analysis of plant extracts active constituent and its research of the mechanism of action Scientific research.Goal in research is that plant extracts can be with its natural characteristic, significant ground effect and high security in Shu Minkang Cosmetic field is stimulated to play due value.

Therefore a kind of safe and efficient, moisturizing and nourishing is provided, be applicable in sensitivity flesh, can significantly releive and repair the combination of impaired skin Object is of great significance.

Summary of the invention

In view of the deficiencies of the prior art and actual demand, for the demand and the deficiencies in the prior art in market, the present invention It provides one kind to releive composition and its preparation method and application, scientifically a variety of natural constituents of combination matching, it is synergistic, it obtains To composition of releiving suitable for various crowds, especially sensitive group, while having effects that moisturizing and nourishing.

To achieve this purpose, the present invention adopts the following technical scheme:

It releives composition in a first aspect, the present invention provides one kind, the composition of releiving includes as follows by weight percent Component: Aloe Barbadensis Miller water 10-50%, cyani flos water 10-30%, Herba polygoni hydropiperis extract 1-7% and ginger-root extract 1- 5%；The sum of mass percent of each component is not more than 100%.

The mass percent of the Aloe Barbadensis Miller water is 10-50%, for example, can be 10%, 12%, 15%, 18%, 20%, 23%, 25%, 27%, 30%, 32%, 35%, 38%, 40%, 43%, 45%, 48% or 50%.

The mass percent of the cyani flos water is 10-30%, for example, can be 10%, 12%, 15%, 18%, 20%, 23%, 25%, 27% or 30%.

The mass percent of the Herba polygoni hydropiperis extract be 1-7%, such as can be 1%, 2%, 3%, 4%, 5%, 6% or 7%.

The mass percent of the ginger-root extract is 1-5%, such as can be 1%, 2%, 3%, 4% or 5%.

In the present invention, use for reference and using theory of traditional Chinese medical science " whole prevention and treatment ", combination matching Aloe Barbadensis Miller water, cyani flos Water, Herba polygoni hydropiperis extract and ginger-root extract, wherein Aloe Barbadensis Miller water, cyani flos water and ginger-root extract, which have, inhibits scorching Disease react the effect of, but said components is individually used for a long time can make dry skin generation microgroove, by inventor's studies have shown that The arrange in pairs or groups Herba polygoni hydropiperis extract of the percentage of Aloe Barbadensis Miller water and cyani flos water can effectively releive skin, further decrease Cell immune response activity, but immunology index is not influenced, it is suitble to various crowds, is particularly suitable for sensitive skin, and the combination The good compatibility of object and cosmetics can apply to multiple skin care products, takees good care of skin, releives and repair impaired skin.

Preferably, the composition of releiving further includes lavandulae flos water.

Preferably, the mass percent that the lavandulae flos water accounts for composition of releiving is 10-30%, such as be can be 10%, 12%, 15%, 18%, 20%, 23%, 25%, 27% or 30%.

In the present invention, remediation composition further includes lavandulae flos water, can nourish the same of skin with neutralization activity oxygen radical When, absorption of the skin to nutriment can be promoted with other components synergy with delaying skin aging.

Preferably, the composition of releiving further includes polysaccharide.

Preferably, the polysaccharide includes any one of mannitol, oat beta-glucan or xylitol or at least two Combination, preferably mannitol.

Preferably, it is 10-30% that the polysaccharide, which accounts for the mass percent of composition, for example, can be 10%, 12%, 15%, 18%, 20%, 23%, 25%, 27% or 30%.

In the present invention, skin barrier is can be enhanced in polysaccharide, protects sensitive skin, while having effects that moisturizing and nourishing, with Aloe, corn flower and ginger-root extract superiority and inferiority are complementary, and moisturizing skin while antiallergic anti-inflammatory prevents water loss.

Preferably, the composition of releiving includes following component by weight percent: Aloe Barbadensis Miller water 10-50%, Cyani flos water 10-30%, Herba polygoni hydropiperis extract 1-7%, ginger-root extract 1-5%, lavandulae flos water 10-30% and polysaccharide 10- 30%；The sum of mass percent of each component is not more than 100%.

Preferably, the composition of releiving includes following component by weight percent: Aloe Barbadensis Miller water 20-50%, Cyani flos water 12-28%, Herba polygoni hydropiperis extract 2-6%, ginger-root extract 2-5%, lavandulae flos water 12-28% and mannitol 12-28%；The sum of mass percent of each component is not more than 100%.

Preferably, the composition of releiving includes following component by weight percent: Aloe Barbadensis Miller water 25-45%, Cyani flos water 15-25%, Herba polygoni hydropiperis extract 3-5%, ginger-root extract 2-4%, lavandulae flos water 15-25% and mannitol 15-20%；The sum of mass percent of each component is not more than 100%.

Second aspect, the present invention provide a kind of method for preparing composition of releiving as described in relation to the first aspect, including as follows Step:

It weighs Aloe Barbadensis Miller water, cyani flos water and lavandulae flos water to be uniformly mixed, filtering, heated at constant temperature, cooling, Herba polygoni hydropiperis extract, ginger-root extract and mannitol is added, is uniformly mixed, composition of releiving is made.

Preferably, the temperature of the heated at constant temperature is 85-95 DEG C, such as can be 85 DEG C, 86 DEG C, 87 DEG C, 88 DEG C, 89 DEG C, 90 DEG C, 91 DEG C, 92 DEG C, 93 DEG C, 94 DEG C or 95 DEG C, preferably 90 DEG C.

Preferably, the time of the heated at constant temperature be 15-25min, such as can be 15min, 16min, 17min, 18min, 19min, 20min, 21min, 22min, 23min, 24min or 25min, preferably 20min.

Preferably, the temperature of the cooling is 40-50 DEG C, such as can be 40 DEG C, 41 DEG C, 42 DEG C, 43 DEG C, 44 DEG C, 45 DEG C, 46 DEG C, 47 DEG C, 48 DEG C, 49 DEG C or 50 DEG C.

The third aspect, the present invention provide a kind of cosmetics, including composition of releiving as described in relation to the first aspect.

Preferably, the cosmetics include lotion, frost, cream or toner.

Preferably, the mass percent of the composition in cosmetics of releiving is 1-15%, such as can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15, preferably 5-10%.

Fourth aspect, the present invention provide a kind of lotion, and the lotion includes composition of releiving as described in relation to the first aspect.

Preferably, the mass percent that the composition of releiving accounts for lotion is 5-8%, such as can be 5%, 6%, 7% Or 8%.

Compared with prior art, the invention has the following beneficial effects:

(1) composition of the present invention of releiving is made of the natural plant composition of plurality of stable, highly-safe, passes through resistance Disconnected allergic reaction occurs or develops, skin is effectively relieved in the multiple pathways such as superfluous free radical in vivo for enhancing body barrier function and removing Skin irritation promotes skin immunity；

(2) composition of releiving of the invention can be compatible with a variety of cosmetics, be added in the cosmetics of different dosage forms character It will not influence cosmetics original function, the smearing sense and use feeling of product can be improved instead, promote the performance of keeping humidity of product；

(3) the environmentally protective development trend of the compound current cosmetics of composition of releiving of the invention, can be used for a long time, and be applicable in Crowd is extensive, especially suitable sensitivity flesh.

Detailed description of the invention

Fig. 1 is that skin moisture content changes comparative diagram in test example 5.

Specific embodiment

Further to illustrate technological means and its effect adopted by the present invention, below by way of specific embodiment come into One step illustrates technical solution of the present invention, but the present invention is not limited in scope of embodiments.

Raw material:

Aloe Barbadensis Miller water is purchased from the green biotech firm in Yunnan ten thousand；

Lavandulae flos water is purchased from French nateva company；

Cyani flos water is purchased from French elixens company；

Mannitol is purchased from Shanghai Yi Chuan chemistry science and technology；

Herba polygoni hydropiperis extract is purchased from BASF Corp. of Germany；

Ginger-root extract is purchased from German Symrise company.

Embodiment 1

One kind is releived composition, by weight percent includes following component:

Aloe Barbadensis Miller water 40%, lavandulae flos water 17%, cyani flos water 20%, mannitol 16%, polygonum flaccidum mention Take object 4% and ginger-root extract 3%.

The composition the preparation method is as follows: weighing Aloe Barbadensis Miller water, cyani flos respectively by above-mentioned mass ratio Water, lavandulae flos water are uniformly mixed and are cooled to 45 DEG C ten minutes later using being heated to 90 DEG C of heat preservations two after 500 mesh filter-cloth filterings, according to Secondary Herba polygoni hydropiperis extract, ginger-root extract and the mannitol that above-mentioned mass percent is added, is uniformly mixed.

Embodiment 2

Aloe Barbadensis Miller water 45%, lavandulae flos water 15%, cyani flos water 18%, mannitol 15%, polygonum flaccidum mention Take object 5% and ginger-root extract 2%.The preparation method is the same as that of Example 1.

Embodiment 3

Aloe Barbadensis Miller water 25%, lavandulae flos water 22%, cyani flos water 25%, mannitol 20%, polygonum flaccidum mention Take object 4% and ginger-root extract 4%.The preparation method is the same as that of Example 1.

Embodiment 4

Aloe Barbadensis Miller water 12%, lavandulae flos water 30%, cyani flos water 30%, xylitol 25%, polygonum flaccidum extract Object 2% and ginger-root extract 1%.The preparation method is the same as that of Example 1.

Embodiment 5

Aloe Barbadensis Miller water 50%, lavandulae flos water 18%, cyani flos water 10%, oat beta-glucan 10%, polygonum flaccidum Extract 7% and ginger-root extract 5%.The preparation method is the same as that of Example 1.

Embodiment 6

Compared with Example 1, other than lavandulae flos water is replaced with deionized water, other conditions are the same as embodiment 1.

Embodiment 7

Compared with Example 1, other than mannitol is replaced with deionized water, other conditions are the same as embodiment 1.

Comparative example 1

Compared with Example 1, other than Aloe Barbadensis Miller water is replaced with deionized water, other conditions are the same as embodiment 1.

Comparative example 2

Compared with Example 1, other than cyani flos water is replaced with deionized water, other conditions are the same as embodiment 1.

Comparative example 3

Compared with Example 1, other than Herba polygoni hydropiperis extract is replaced with deionized water, other conditions are the same as embodiment 1.

Comparative example 4

Compared with Example 1, other than ginger-root extract is replaced with deionized water, other conditions are the same as embodiment 1.

Comparative example 5

100% Aloe Barbadensis Miller water.

Comparative example 6

Compared with Example 1, only with one-component lavandulae flos water, i.e. 100% lavandulae flos water, other conditions are the same as real Apply example 1.

Comparative example 7

Compared with Example 1, only with one-component cyani flos water, i.e. 100% cyani flos water, other conditions are the same as real Apply example 1.

Comparative example 8

Compared with Example 1, only with one-component Herba polygoni hydropiperis extract, i.e. 100% Herba polygoni hydropiperis extract, other conditions are the same as real Apply example 1.

Comparative example 9

Compared with Example 1, only with one-component ginger-root extract, i.e. 100% ginger-root extract, other conditions are the same as real Apply example 1.

Comparative example 10

A kind of composition, Aloe Barbadensis Miller water including mass percent 70% and 30% Herba polygoni hydropiperis extract.

1 hyaluronidase Inhibition test of test example

1, experimental principle: hyaluronidase is anaphylactoid participant, research shows that hyaluronidase and inflammation, allergy There is strong correlation.For antiallergic activity using hyaluronic acid enzyme inhibition rate as standard, the more big then antiallergy of hyaluronic acid enzyme inhibition rate is living Property is stronger.

2, experimental procedure:

2.1 solution are prepared

2.1.1 hac buffer (pH=5.6)

(1) 1155 μ L glacial acetic acids are measured and with ultrapure water are diluted to 100mL, taken after mixing wherein 4.8mL be solution A.

(2) it weighs the crystallization of 2.72g sodium acetate to be dissolved in ultrapure water, is settled to 100mL with ultrapure water, is taken wherein after mixing 45.2mL being B solution.

(3) mixed solution A, B are settled to 100mL with ultrapure water, mix.Its pH value of micrometric measurement, is adjusted to solution A or B 5.6.

2.1.2 hyaluronidase solution

It weighs hyaluronidase 0.0100g to be placed in a beaker, the dissolution of 4mL hac buffer is added.

2.1.3 sodium hyaluronate solution (0.5mg/mL)

It weighs Sodium Hyaluronate 0.01g to be placed in a beaker, the dissolution of 20mL hac buffer is added.

2.1.4 Ehrlich's reagent (Ehrlich reagent)

It weighs the p- dimethylaminobenzaldehyde of 0.8g to be dissolved in 15mL concentrated hydrochloric acid and 15mL dehydrated alcohol, can be reserved for two months.

2.1.5 sodium carbonate liquor (1.0mol/L)

5.3g sodium carbonate is weighed, is dissolved with ultrapure water and is settled to 50mL.

2.1.6 acetylacetone,2,4-pentanedione solution

Acetylacetone,2,4-pentanedione 3.5mL is taken to be dissolved in the sodium carbonate liquor of 50mL 1.0mol/L, matching while using.

2.1.7 calcium chloride solution (0.25mmol/L)

0.02775g calcium chloride is weighed, is dissolved with ultrapure water and is settled to 1000mL.

2.1.8 sodium hydroxide solution (0.4mol/L)

1.6g calcium chloride is weighed, is dissolved with ultrapure water and is settled to 100mL.

The measurement of 2.2 hyaluronidase activity inhibiting rates

Selection example 1-7 and comparative example 1-10 are performed the following operation as sample:

(1) take 0.1mL 0.25mmol/L calcium chloride solution and 0.5mL hyaluronidase solution in 37 DEG C of heat insulating cultures 20min；

(2) sample liquid 0.5mL is added, continues 37 DEG C of heat insulating culture 20min；

(3) 0.5mL sodium hyaluronate solution, 37 DEG C of heat insulating culture 30min, room temperature 5min are added；

(4) 0.1mL 0.4mol/L sodium hydroxide solution and 0.5mL acetylacetone,2,4-pentanedione solution is added, is placed in boiling water bath and heats After 15min, cooling 5min is carried out with ice water immediately；

(5) Ehrlich's reagent 1.0mL is added, and is diluted with 3.0mL dehydrated alcohol, room temperature 20min colour developing, Stand-by spectrophotometric determination light absorption value；

(6) length scanning that 450-700nm range is carried out to sample, to determine maximum absorption wavelength, in the absorption maximum Light absorption value measurement is carried out at wavelength, the results are shown in Table 1.

3, interpretation of result

Hyaluronic acid enzyme inhibition rate (%)=[(A-B)-(C-D)]/(A-B) × 100

In formula: A is the light absorption value of blank group, and blank group, which refers to, replaces sample solution with hac buffer；

B is the light absorption value of blank control group, and blank control group, which refers to, replaces sample solution and enzyme solution with hac buffer；

C is the light absorption value of sample sets；

D is the light absorption value of sample controls group, and sample controls group, which refers to, replaces enzyme solution with hac buffer.

1 hyaluronic acid enzyme inhibition rate of table

As shown in Table 1, comparing embodiment 1 and embodiment 6-7, show that the missing of lavandulae flos water or polysaccharide can reduce combination Inhibitory effect of the object to hyaluronidase；Comparing embodiment 1 and comparative example 1-4 are it is found that Aloe Barbadensis Miller water, cyani flos Water, Herba polygoni hydropiperis extract and ginger-root extract are indispensable, and the missing of any component reduces suppression of the composition to hyaluronidase Production is used；Comparing embodiment 1 and comparative example 5-9 is it is found that Aloe Barbadensis Miller water, cyani flos water, the polygonum flaccidum of one-component mention The activity of hyaluronidase cannot effectively be inhibited by taking object, ginger-root extract, lavandulae flos water or polysaccharide, but of the present invention Under conditions of mixture ratios, each component is synergistic, significantly improves composition to the inhibiting rate of hyaluronidase, antiallergic effect is obvious；Than Compared with embodiment 1 and comparative example 10 it is found that the combination of Aloe Barbadensis Miller water and Herba polygoni hydropiperis extract is for hyaluronic acid enzyme inhibition rate It is not significant.This test example explanation, could only obtain antiallergic significant effect under constituent species of the present invention and conditions of mixture ratios Composition of releiving.

Application Example 1-3

The lotion for reparation of releiving is prepared using optimum embodiment 1 of the present invention, specific formula is shown in Table 2.

Table 2 is releived the emulsion formulations of reparation

Application Example 1-3's the preparation method is as follows:

(1) A phase raw material is heated to 80 DEG C, be dispersed with stirring uniformly, for use；

(2) B phase raw material is heated to 80 DEG C, stirring and dissolving is uniform；

(3) it takes out B phase raw material to enter A phase and carry out emulsifying, cool down after emulsification；

(4) 45 DEG C of addition C phase raw materials are cooled to, are dispersed with stirring uniformly, vacuumizing and defoaming finishes.

Comparison study example 1-7

Using lotion described in Comparison study example 1-7 as control, specific formula is shown in Table 3.

The emulsion formulations of 3 Comparison study example of table

Preparation method is the same as Application Example 1-3.

The experiment of 2 erythrocyte hemolysis of test example

The lotion of lotion and Comparison study example 1-7 preparation prepared by Application Example 1-3 is as test specimen.

1, test principle: erythrocyte hemolysis experiment is the cell of lipid and albumen and red blood cell based on human skin surface The similar principle of film, the influence by detection cosmetics to hemoglobin release and the hemoglobin denaturation of red blood cell, to evaluate The irritation degree of cosmetics.

2, experiment condition

2.1 red cell suspension

The sheep red blood cell (SRBC) suspension that concentration is 2%, sterile blood sampling are saved with Alsever's Solution, are placed in (shelf-life 1,4 DEG C of refrigerators Month, it is proposed that used in 2 weeks).

2.2 reagent

Lauryl sodium sulfate (SDS), dimethyl sulfoxide (DMSO), phosphate buffer (PBS), physiological saline, ethyl alcohol.

2.3 instrument and equipment

Microplate reader, constant incubator, ultrapure water machine, high-pressure steam sterilizing pan, pipettor, 4 DEG C of refrigerators, centrifuge, electric heating Thermostat water bath, assay balance.

3, operating procedure

The preparation of 3.1 sample suspensions

3.1.1 solvent selects: it is general to select physiological saline or PBS as solvent, for insoluble in physiological saline or PBS Sample DMSO or ethyl alcohol can be selected is solvent.It must assure that each dilute sample suspension mixes well.Sample concentration range detection Experiment: tested material is made into a series of gradients such as 0.001%, 0.01%, 0.1%, 1%, 10%, 20%, 30%, 40% with solvent Suspension, with red cell suspension in equal volume than mixing, centrifuging and taking supernatant after 37 DEG C of culture 3h, in microplate reader 410nm, 540nm, Absorbance is measured at 575nm, and is compared with the light absorption value of negative control and positive control.According to as a result, covering haemolysis The interval selection suitable concentration up and down of rate 50%, as sample formal test concentration range.

3.1.2 the preparation of sample suspension: according to detection experimental result, appropriate test concentrations are selected, it is dense to prepare sample series Degree.

3.2 haemolysis and albuminous degeneration measurement

In 2mL centrifuge tube, the 500 μ L of tested material and 2% sheep red blood cell (SRBC) suspension, 500 μ L that various concentration is added (wait bodies Product mixing), it mixes well.Each concentration is respectively provided with 3 in parallel.It is (i.e. complete as negative control using solvent (physiological saline or PBS) Complete not haemolysis), using 0.1%SDS solution as positive control (i.e. complete hemolysis).After being placed in 37 DEG C of constant incubators incubation 3h, 2000rpm/min is centrifuged 3min, 10 μ L of Aspirate supernatant, be added in ELISA Plate (prior every hole plus 90 μ L physiological saline or PBS), mix, absorbance (A is measured at microplate reader 410nm, 540nm, 575nm_410nm、A_540nm、A_575nm)。

4, interpretation of result

The calculation method of 4.1 haemolysis measurement

It is calculated according to the following formula hemolysis rate (%): hemolysis rate (%)=(A-C)/(B-C) × 100；

In formula, A is mean light absorbency of each concentration of sample at 410nm, and B is average suction of the positive control at 410nm Luminosity, C are mean light absorbency of the negative control at 410nm.

Determine that tested material causes the tested material concentration HC50 (mg/L) of 50% haemolysis of red blood cell according to regression equation.

Albuminous degeneration index D I (%) is calculated according to the following formula in the calculation method of 4.2 albuminous degenerations measurement:

DI (%)=(tested material A_575nm/A_540nm)/(positive control A_575nm/A_540nm)×100。

5, judgment criteria

The L/D value of sample is calculated according to HC50 value and DI value: L/D=HC50/DI the results are shown in Table 4；According to European alternative The RBC of method authentication center (ECVAM) tests grade scale, to cosmetics irritation grading, as shown in table 5.

4 erythrocyte hemolysis experimental result of table

Sample ID	HC50(mg/L)	DI (%)	L/D	Evaluation
					Application Example 1	53923	92.25	584.53	It is nonirritant
Application Example 2	57383	92.98	617.15	It is nonirritant
					Application Example 3	81024	93.36	867.87	It is nonirritant
Comparison study example 1	151	90.11	1.68	Slight stimulation
					Comparison study example 2	9879	92.55	106.74	It is nonirritant
Comparison study example 3	8098	91.44	88.56	Microstimulation
					Comparison study example 4	6229	91.63	67.98	Microstimulation
Comparison study example 5	1687	91.57	18.42	Microstimulation
					Comparison study example 6	7436	92.04	80.79	Microstimulation
Comparison study example 7	8680	92.90	93.43	Microstimulation

5 ECVAM cosmetic product RBC of table tests irritation grade scale

L/D	Classification
		﹥ 100	It is nonirritant
10 L/D≤100 ﹤	Microstimulation
		1 L/D≤10 ﹤	Slight stimulation
0.1 L/D≤1 ﹤	Moderate irritation
		L/D≤0.1	Severe irritation

As shown in Table 4, compare half erythrocyte hemolysis concentration (HC50), Application Example is far superior to Comparison study Example illustrates that antiallergic remediation composition has good antiallergic repairing effect, and antiallergic remediation composition additive amount is higher, and effect is got over It is good.Add lotion (Comparison study example 2, Comparison study example 3, Comparison study example 4, the application of single antiallergic remediation composition component Comparative example 5, Comparison study example 6 and Comparison study example 7) its L/D value less adds the lotion (Comparison study of antiallergic remediation composition Example 1) also there is certain raising, but improvement effect is far away from composition.Test result illustrates that the antiallergic composition of releiving is to need Specifically science combination and proportion it can be only achieved apparent effect.

The experiment of 3 patch of test example

The lotion of Application Example 1 is taken, control sample is Comparison study example 1, is with 30 women and 20 males respectively Object takes 0.03ml sample to be placed in the coyote hole of spot examination adhesive tape, the spot for being equipped with sample is tried adhesive tape from lower part immediately with syringe Start it is longitudinal is affixed on the normal skin of left forearm, while light pressing room and is uniformly distributed trier one by one to drive away air. Experiment position marks, to observe.It is primary every 24 hours patch skins, half an hour after patch is opened, then is referred to according to CTFA The term in south is checked.6 patch experiments have been carried out altogether, paste the 6th day after skin, in order to observe delayed response, into Additional inspection is gone.Data then utilize last time inspection result, compare.The standard of perfection of skin irritation test is shown in The following table 6, skin irritation test the results are shown in Table 7.

6 skin irritation standard of perfection of table

Grade	Symbol	Standard of perfection
			0	-	Negative reaction: non-stimulated, without erythema
1	±	Suspicious reaction: slight erythema
			2	+	Weakly positive reaction: erythema
3	++	Strong positive reaction: erythema, papule, blister
			4	+++	Extremely strong positive reaction: serious edema, bulla

7 skin irritation test result of table

The results show that lotion of the Application Example 1 containing antiallergic remediation composition of the present invention is non-stimulated to skin, it is no positive anti- It answers, and there is multiple positive reaction in Comparison study example 1.Illustrate that the composition can be obviously improved the mildness of product.

4 sensory evaluation of test example test

According to Organoleptic method in cosmetic industry, lotion made from Application Example 1 and Comparison study example 1 is chosen, into Row skin sense assessment.

1, assessment item: spreadability, absorbability, mildness, overall effect.

2, grading system: score value is 1~10 point, and score value is higher, and the project indicator characteristic is stronger.

3, experimental program: 10 appraisers trained of selection, women, the age 25-35 years old.In constant temperature freestanding environment (22 ± 1 DEG C), relative humidity 50 ± 5% carry out after 1 hour of resting, the results are shown in Table 8.

8 Analyses Methods for Sensory Evaluation Results of table

	Application Example 1	Comparison study example 1
			Spreadability	9.0	7.0
Moisture retention	9.0	6.5
			Absorbability	8.5	6.8
Mildness	9.0	4.5
			Overall effect	9.0	6.0

For the lotion that can be seen that Application Example 1 from the evaluation result of user applies 1 lotion of comparative example relatively, 4 Item evaluation index is comprehensively leading, and especially tool improves significantly in terms of mildness.Illustrate under comparable conditions, containing the present invention Its use feeling of the lotion of antiallergic of releiving composition is an advantage over ordinary emulsion, spreadability, moisture retention, absorbability, mildness and whole Body effect is all more excellent.At the same time, composition of releiving of the invention also can effectively solve the problems such as skin general red, erythema, comprehensive Significant effect.

The test of 5 humidity-preserving type of test example

According to cosmetic industry standard " cosmetics moisture-keeping efficacy evaluation guide ", 20 volunteers are chosen, by random table at it Double forearms use lotion made from Application Example 1 and Comparison study example 1 respectively, and skin is carried out when T0, T30min and T2h and is contained Measure of water, so that the moisture-keeping efficacy to product in skin surface is assessed.Experimental result is shown in Fig. 1 and tables 9.

9 hydrauture change rate result of table

By table 9 and Fig. 1 it is found that the moisture value of Application Example 1 increases 49.03%, significantly after use after 30 minutes Better than the 29.98% of Comparison study example 1；After 2h, the moisture value change rate of Application Example 1 is 37.50%, same significant Better than the 11.50% of Comparison study example 1, illustrate that remediation composition of releiving of the invention also has preferable promotion product moisture retention The characteristic of energy.

In conclusion composition of the invention has selected aloe, corn flower, race that inflammatory reaction can be inhibited to occur etc. to mention Take object, enhance the polysaccharide component such as mannitol of skin barrier function, and can with the lavender of neutralization activity oxygen radical at Point, and the polygonum flaccidum ingredient that can be reduced cell immune response activity but not influence immunology index is added, the proportion by science is surveyed Examination, finally obtain can comprehensive reparation sensitivity skin remediation composition of releiving, and the composition can be used for skin care item Preparation, do not influencing the character of existing product while the skin that can releive, inhibiting inflammation, target user is extensive, especially Suitable for sensitive flesh crowd.

The Applicant declares that the present invention is explained by the above embodiments method detailed of the invention, but the present invention not office Be limited to above-mentioned method detailed, that is, do not mean that the invention must rely on the above detailed methods to implement.Technical field Technical staff it will be clearly understood that any improvement in the present invention, equivalence replacement and auxiliary element to each raw material of product of the present invention Addition, selection of concrete mode etc., all of which fall within the scope of protection and disclosure of the present invention.

Claims

The composition 1. one kind is releived, which is characterized in that the composition of releiving includes following component: Curacao by weight percent Chinese aloe leaf water 10-50%, cyani flos water 10-30%, Herba polygoni hydropiperis extract 1-7% and ginger-root extract 1-5%；The each component The sum of mass percent be not more than 100%.
2. composition according to claim 1 of releiving, which is characterized in that the composition of releiving further includes lavandulae flos Water；

Preferably, it is 10-30% that the lavandulae flos water, which accounts for the mass percent for composition of releiving,.
3. composition according to claim 1 or 2 of releiving, which is characterized in that the composition of releiving further includes polysaccharide；

Preferably, the polysaccharide includes the combination of any one of mannitol, oat beta-glucan or xylitol or at least two, Preferably mannitol；

Preferably, it is 10-30% that the polysaccharide, which accounts for the mass percent of composition,.
4. composition according to claim 1-3 of releiving, which is characterized in that the composition of releiving is by quality hundred Score includes following component: Aloe Barbadensis Miller water 10-50%, cyani flos water 10-30%, Herba polygoni hydropiperis extract 1-7%, race Extract 1-5%, lavandulae flos water 10-30% and polysaccharide 10-30%；The sum of the mass percent of each component is not more than 100%.
5. composition of releiving described in any one of -4 according to claim 1, which is characterized in that the composition of releiving is by quality Percentage includes following component: Aloe Barbadensis Miller water 20-50%, cyani flos water 12-28%, Herba polygoni hydropiperis extract 2-6%, ginger Root extract 2-5%, lavandulae flos water 12-28% and mannitol 12-28%；The mass percent of each component is not more than 100%；The sum of mass percent of each component is not more than 100%.
6. composition according to claim 1-5 of releiving, which is characterized in that the composition of releiving is by quality hundred Score includes following component: Aloe Barbadensis Miller water 25-45%, cyani flos water 15-25%, Herba polygoni hydropiperis extract 3-5%, race Extract 2-4%, lavandulae flos water 15-25% and mannitol 15-20%；The sum of mass percent of each component is little In 100%.
7. a kind of method for preparing composition as claimed in any one of claims 1 to 6 of releiving, which is characterized in that weigh Curacao Chinese aloe leaf water, cyani flos water and lavandulae flos water are uniformly mixed, filtering, heated at constant temperature, and Herba polygoni hydropiperis extract, ginger is added in cooling Root extract and mannitol are uniformly mixed, and composition of releiving is made；

Preferably, the temperature of the heated at constant temperature is 85-95 DEG C, preferably 90 DEG C；

Preferably, the time of the heated at constant temperature is 15-25min, preferably 20min；

Preferably, the temperature of the cooling is 40-50 DEG C.
8. a kind of cosmetics, which is characterized in that including composition as claimed in any one of claims 1 to 6 of releiving；

Preferably, the cosmetics include lotion, frost, cream or toner.
9. cosmetics according to claim 8, which is characterized in that the quality percentage of the composition in cosmetics of releiving Number is 1-15%, preferably 5-10%.
10. a kind of lotion, which is characterized in that the lotion includes composition described in any one of claims 1-6 of releiving；

Preferably, it is 5-8% that the composition of releiving, which accounts for the mass percent of lotion,.