CN103202796B - Skin care composition and face cream with acne treatment efficacy and preparation method of face cream - Google Patents

Abstract

The invention provides a face cream with acne treatment efficacy and a preparation method of the face cream. The face cream is prepared by taking traditional Chinese medicine theory of China as a basis, taking skin health as a purpose, taking a composite traditional Chinese medicine extract as a main efficacy additive and taking common substrate components in cosmetics as assist. The acne treatment face cream provided by the invention has bacteriostasis efficacy, and also has the efficacies of removing pathogenic heat from blood, promoting blood, reducing the stagnation, relieving itching, diminishing inflammation and the like. The composite traditional Chinese medicine extract is naturally green, healthy and safe, and can be widely applied to acne treatment cosmetics.

Description

There is the skin care compositions of acne-removing, facial cream and preparation method thereof

Technical field

The present invention relates to a kind of skin care compositions, specifically a kind of have anti-acne and the Chinese medicine composition having bacteriostasis efficacy concurrently and the Chinese medicinal skin care facial cream prepared by this skin care compositions.

Background technology

Comedo is that hair follicle and sebaceous gland are blocked, and a kind of chronic inflammatory skin that inflammation causes is sick, is common in the youth of both sexes of adolescence, mainly occur in face, cervical region, chest and back, shoulder and upper arm, form pimple, the traditional Chinese medical science claims " acne ", and doctor trained in Western medicine name of disease is " acne ".Adolescence androgen increases, and smegma increases, and makes the different hyperkeratosis of hair follicle, sebaceous gland simultaneously, and the flat dead cell that horny layer comes off falls hair follicle infundibulum, is combined with sebum, produces mixture.If these mixture freely can not discharge skin surface, and deposit form fat bolt in hair follicle.When these dyskeratosis cell aggregationes, when lower hopper expands, just form ripe acne.Sebum is by the bacterial decomposition existed in hair follicle, and produce fatty acid, the latter's hair follicle stimulating causes inflammation, and causes follicular wall lesion ruptures, and hair follicle content enters corium, thus, cause the inflammatory reaction of perifollicolar varying degree.The genesis mechanism of comedo, relevant with following three large factors:

(1) to the agnogenio property aggravation that androgenic steroids (as testosterone, dehydroepiandros-sterone, hydroxyprogesterone) reacts, cause sebum to produce and increase.

(2) propionibacterium acnes hypertrophy.This bacterium is hair follicle normal flora composition, and it, and makes it to be partially converted into free fatty for it acts on machine matter with sebum.Propionibacterium acnes also can activating complement path, at least produces two kinds of chemotactic factors, impels inflammation to occur.

(3) capsular epithelium cell quantity increases, and has tackness, forms hair follicle tetention keratosis excessive.The latter's occurrence cause is undistinct yet.Propionibacterium acnes produces free fatty, may be the principal element that comedo is formed.

There is following problem in product for resolving poxes popular on the market at present: 1, easily cause skin allergy, large to skin irritation; 2, add chemical antibacterial and destroy skin normal flora; 3, some banned substances are added as hormones such as chloromycetin; 4, heavy metal substance is added.

Summary of the invention

The object of the invention is to propose a kind of skin care compositions with acne-removing.

Another object of the present invention is the preparation method proposing a kind of above-mentioned skin care compositions.

The third object of the present invention is the skin protection facial cream proposing to utilize above-mentioned preparation method to prepare.

Invention thinking of the present invention is:

The present invention utilizes advanced biotechnology, in conjunction with the theory of Chinese medical science of Chinese tradition, Herba Artemisiae Scopariae, the Fructus Kochiae, Cortex Moutan, Galla Chinensis, Spina Gleditsiae, Spica Prunellae are effectively combined, form compound preparation, and it can be used as active component, be prepared into Pimple removing scream, make it have antibacterial, short infiltration, the effect of heat-clearing and toxic substances removing, promoting blood circulation and detumescence, thus effectively can prevent and treat dissimilar acne, give the new life of skin and vigor.

For realizing object of the present invention, the present invention adopts following concrete technical scheme:

Have a skin care compositions for acne-removing, described skin care compositions comprises A phase and B phase, and described A phase is made up of the raw material of following weight percentage ratio:

Described B phase is made up of following materials by weight percentage:

Above-mentioned a kind of skin care compositions with acne-removing, wherein said Chinese medicine extract is obtained through the extraction of field of Chinese medicines general extraction methods by the crude drug of following weight:

Herba Artemisiae Scopariae 20 ~ 30, the Fructus Kochiae 20 ~ 30, Cortex Moutan 10 ~ 20, Galla Chinensis 10 ~ 20, Spina Gleditsiae 10 ~ 20, Spica Prunellae 10 ~ 20.

Preferably, above-mentioned skin care compositions, wherein said Chinese medicine extract is obtained through following method extraction by the crude drug of following weight:

Herba Artemisiae Scopariae 20 ~ 30, the Fructus Kochiae 20 ~ 30, Cortex Moutan 10 ~ 20, Galla Chinensis 10 ~ 20, Spina Gleditsiae 10 ~ 20, Spica Prunellae 10 ~ 20;

Described method is that step is as follows:

(1) according to above-mentioned weight weighting raw materials;

(2) percent by volume is 75% ~ 80% ethanol extraction twice, and it is 1:10 ~ 1:20 that first time extracts crude drug and ethanol mass volume ratio g/ml, 60 DEG C ~ 70 DEG C heated and stirred 1 ~ 2 hour, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:10 ~ 1:20,60 DEG C ~ 70 DEG C heated and stirred 30min ~ 60min, filters to obtain second time filtrate; Filtrate and the mixing of second time filtrate, be concentrated into 0.2 ~ 0.5g/ml, obtain concentrate for the first time;

(3) add propylene glycol or butanediol, sucking filtration according in the mass ratio concentrate that to be 1:1 ~ 1:2 obtain to step (2), 3000 ~ 5000 revs/min of centrifuging and taking supernatant, to obtain final product.

Above-mentioned a kind of skin care compositions with acne-removing, wherein, described compositions also comprises C phase, and described C phase is antiseptic 0.05 ~ 0.2wt%.

A preparation method for above-mentioned skin care compositions, described method comprises the steps:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese medicine extract are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 80 ~ 85 DEG C, mixing;

(2) A phase: weigh each raw material of A phase according to ratio requirement, heat 80 ~ 85 DEG C, mixing;

(3) A phase is all joined in B phase, 2000 ~ 3000 revs/min, homogenizing 5min ~ 10min; 30 ~ 50 revs/min, after stirring 5 ~ 10min, start to stir cooling, speed 30 ~ 50 revs/min, rate of temperature fall is 1 ~ 2 DEG C/min;

(4) when temperature drops to below 45 ~ 50 DEG C, add Chinese medicine extract, stir, continue to be cooled to less than 40 DEG C, to obtain final product.

A preparation method for above-mentioned skin protection compositions, wherein, described method comprises the steps:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese medicine extract are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 80 ~ 85 DEG C, mixing;

(2) A phase: weigh each raw material of A phase according to ratio requirement, heat 80 ~ 85 DEG C, mixing;

(3) A phase is all joined in B phase, 2000 ~ 3000 revs/min, homogenizing 5min ~ 10min; 30 ~ 50 revs/min, after stirring 5 ~ 10min, start to stir cooling, speed 30 ~ 50 revs/min, rate of temperature fall is 1 ~ 2 DEG C/min;

(4) for when temperature drops to below 45 ~ 50 DEG C, add Chinese medicine extract and C phase antiseptic, stir, continue to be cooled to less than 40 DEG C, to obtain final product.

Have a skin care formulation for acne-removing, described skin care formulation is made up of said method.

The preparation that above-mentioned raw materials and method prepare is Pimple removing scream.Different according to use approach, Chinese medicine composition of the present invention makes powder after can simply pulverizing, and pats skin, also can realize final technique effect.Also other external-use skin care preparations can be made, as cleansing milk, spray or essence etc. through extracting as additive.But be not limited to above-mentioned dosage form.

Facial cream recommendation method of the present invention is: after clean of every day, Pimple removing scream is coated on the skin of pox, massages gently, treat that facial cream slowly absorbs.

Beneficial outcomes of the present invention:

Raw material of the present invention all selects natural plant composition, confirms non-stimulated to skin safe through experiment, fundamentally can improve skin microenvironment and reach the effect of curing acne, and have clearing away heat and cooling blood concurrently, blood circulation promoting and dispersing pathogen accumulation, antipruritic, press down the effects such as fat antiinflammatory, can also alleviate and eliminate cicatrix pockmark etc.Raw material of the present invention obtains easily, preparation method is easy, and green safety, anti-acne effect is good, is easy to be accepted by consumers in general, has good application prospect and market prospect.

Accompanying drawing explanation

Skin oil and fat changes of contents situation after Fig. 1 Pimple removing scream sample uses;

The situation of change of skin oil and fat increment rate after Fig. 2 Pimple removing scream sample uses;

The relative increment rate of skin oil and fat after Fig. 3 Pimple removing scream sample uses;

Fig. 4 Pimple removing scream is to the inhibition of pimple;

Fig. 5 Pimple removing scream is to the inhibition of pustule;

Fig. 6 first week volunteer's in-service evaluation effect;

Fig. 7 second week volunteer in-service evaluation effect;

Fig. 8 the 3rd week volunteer's in-service evaluation effect;

Fig. 9 4th week volunteer in-service evaluation effect;

After Figure 10 Pimple removing scream uses surrounding, volunteer produces evaluation of effect to preventing pockmark.

Detailed description of the invention

The present embodiment medical material used all can be bought from China Medicament Group Corp. and obtain, and meets Pharmacopoeia of People's Republic of China 2005 editions standards.Other raw materials all obtain by commercially available purchase, and the present invention uses the source of raw material in table 1.

Table 1

The preparation of embodiment 1 facial cream of the present invention

Raw material and proportioning (mass percent), in table 2:

Table 2

The preparation of Chinese medicine extract:

(1) according to following weight parts proportioning weighting raw materials;

Herba Artemisiae Scopariae 30g, Fructus Kochiae 20g, Cortex Moutan 10g, Galla Chinensis 20g, Spina Gleditsiae 10g, Spica Prunellae 20g.

(2) percent by volume is 75% ethanol extraction twice, and it is 1:10 that first time extracts crude drug and ethanol mass volume ratio g/ml, 60 DEG C of heated and stirred 1 hour, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:10,60 DEG C of heated and stirred 30min, filters to obtain second time filtrate; First time, filtrate mixed with second time filtrate, and rotary evaporation is concentrated into 0.2g/ml, obtains concentrate;

(3) redissolve according to adding propylene glycol in the mass ratio concentrate that to be 1:2 obtain to step (2), sucking filtration, 3000 revs/min of centrifuging and taking supernatant, collect filtrate, to obtain final product.

Facial cream preparation method:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese prescription extracting solution are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 80 DEG C;

(2) A phase: weigh A phase raw material according to ratio requirement, heat 85 DEG C;

(3) A phase is all joined in B phase, 2000 revs/min, homogenizing 5min; 30 revs/min, after stirring 5min, start to stir cooling, speed 30 revs/min, rate of temperature fall is 1 DEG C/min;

(4) when temperature drops to below 45 DEG C, add Chinese medicine extract, stir; When temperature drops to less than 40 DEG C, described facial cream.

The preparation of embodiment 2 facial cream of the present invention

Raw material and proportioning (mass percent), in table 3:

Table 3

The preparation method of Chinese medicine extract:

(1) according to following weight parts proportioning weighting raw materials;

Herba Artemisiae Scopariae 20g, Fructus Kochiae 30g, Cortex Moutan 20g, Galla Chinensis 10g, Spina Gleditsiae 15g, Spica Prunellae 15g.

(2) percent by volume is 80% ethanol extraction twice, and it is 1:20 that first time extracts crude drug and ethanol mass volume ratio g/ml, 70 DEG C of heated and stirred 2 hours, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:20,70 DEG C of heated and stirred 60min, filters to obtain second time filtrate; First time, filtrate mixed with second time filtrate, and rotary evaporation is concentrated into 0.3g/ml, obtains concentrate;

(3) add butanediol, sucking filtration, 5000 revs/min of centrifuging and taking supernatant according in the mass ratio concentrate that to be 1:1 obtain to step (2), collect filtrate, to obtain final product.

The preparation method of facial cream:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese prescription extracting solution are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 85 DEG C;

(2) A phase: weigh A phase raw material according to ratio requirement, heat 80 DEG C;

(3) A phase is all joined in B phase, 3000 revs/min, homogenizing 10min; 50 revs/min, after stirring 10min, start to stir cooling, speed 50 revs/min, rate of temperature fall is 2 DEG C/min;

(4) when temperature drops to below 50 DEG C, add Chinese prescription extracting solution, stir; When temperature drops to less than 40 DEG C, to obtain final product.

The preparation of embodiment 3 facial cream of the present invention

Raw material and proportioning (mass percent), in table 4:

Table 4

Chinese medicine extract preparation method:

(1) according to following weight parts proportioning weighting raw materials;

Herba Artemisiae Scopariae 25g, Fructus Kochiae 25g, Cortex Moutan 15g, Galla Chinensis 15g, Spina Gleditsiae 20g, Spica Prunellae 10g.

(2) percent by volume is 75% ethanol extraction twice, and it is 1:15 that first time extracts crude drug and ethanol mass volume ratio g/ml, 65 DEG C of heated and stirred 1.5 hours, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:15,65 DEG C of heated and stirred 40min, filters to obtain second time filtrate; First time, filtrate mixed with second time filtrate, and rotary evaporation is concentrated into 0.5g/ml, obtains concentrate;

(3) add butanediol, sucking filtration, 5000 revs/min of centrifuging and taking supernatant according in the mass ratio concentrate that to be 1:1 obtain to step (2), collect filtrate, to obtain final product.

Facial cream preparation method:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese prescription extracting solution are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 85 DEG C;

(2) A phase: weigh A phase raw material according to ratio requirement, heat 80 DEG C;

(3) A phase is all joined in B phase, 2500 revs/min, homogenizing 8min; 40 revs/min, after stirring 8min, start to stir cooling, speed 40 revs/min, rate of temperature fall is 1 DEG C/min;

(4) when temperature drops to below 48 DEG C, add Chinese prescription extracting solution and C phase raw material, stir; When temperature drops to less than 40 DEG C, to obtain final product.

The preparation of embodiment 4 facial cream of the present invention

Raw material and proportioning (mass percent), in table 5:

Table 5

The preparation of Chinese medicine extract:

(1) according to following weight parts proportioning weighting raw materials;

Herba Artemisiae Scopariae 20g, Fructus Kochiae 20g, Cortex Moutan 10g, Galla Chinensis 10g, Spina Gleditsiae 20g, Spica Prunellae 10g.

(2) percent by volume is 75% ethanol extraction twice, and it is 1:20 that first time extracts crude drug and ethanol mass volume ratio g/ml, 60 DEG C of heated and stirred 1 hour, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:20,60 DEG C of heated and stirred 30min, filters to obtain second time filtrate; First time, filtrate mixed with second time filtrate, and rotary evaporation is concentrated into 0.4g/ml, obtains concentrate;

(3) add butanediol, sucking filtration, 5000 revs/min of centrifuging and taking supernatant according in the mass ratio concentrate that to be 1:1 obtain to step (2), collect filtrate, to obtain final product.

Facial cream preparation method:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese prescription extracting solution are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 83 DEG C;

(2) A phase: weigh A phase raw material according to ratio requirement, heat 83 DEG C;

(3) A phase is all joined in B phase, 3000 revs/min, homogenizing 5min; 50 revs/min, after stirring 5min, start to stir cooling, speed 50 revs/min, rate of temperature fall is 1 ~ 2 DEG C/min;

(4) when temperature drops to below 50 DEG C, add Chinese prescription extracting solution and C phase raw material, stir; When temperature drops to less than 40 DEG C, to obtain final product.

Efficacy experiments of the present invention

One, Pimple removing scream anti-acne effect

1. inhibition zone

1) experimental principle

Inoculating in the culture medium for examination strain, imposing quantitative embodiment 1 and preparing sample, utilizing the scattering and permeating effect of sample in agar culture medium, after certain hour, sample permeates diffusion reaches certain limit.In the scope that Mlc reaches, the growth of indicator bacteria is just suppressed, produces an aseptic transparent region, is called inhibition zone.Under certain condition with in certain limit, the diameter of inhibition zone and the logarithm of sample concentration linearly functional relationship.Utilize this principle, the size of antibacterial virulence can be compared, or the content of working sample effective ingredient.The inhibition zone size of parallel 1, parallel 2, parallel 3 representative samples, one group of experimental data will do three parallel laboratory tests, last experimental result be get three parallel numerical value meansigma methods as last result.

2) the inhibition zone result of antibacterial experiment in vitro

Table 6

The criterion of In Vitro Bacteriostasis qualitative results: it is insensitive (-) that antibacterial circle diameter is less than 8mm, 8 ~ 13mm is less sensitive (+), and 13 ~ 19mm is medium sensitivity (++), and more than 19mm is extremely sensitive (+++).The embodiment of the present invention 1 is prepared Chinese medicine extract and is had good fungistatic effect, and inventor utilizes other embodiments to prepare sample and repeats above-mentioned experiment, and result is with embodiment 1.

2, product control oil effect

1) experimental principle

Human experimentation, by particular experiment, crowd forms test population, and test subject makes to apply some make up the change of skin oil and fat content before and after (and cosmetic industry composition), thus deterministic cosmetic (or functional component) press down fat effect.

2) experimental apparatus and sample

Experimental apparatus: skin oil and fat tester sM815.

Sample: facial cream prepared by embodiment 1.

Blank: recipient site does not use any cosmetics.

3) Subject Population

Experimenter amounts to 30 people.Concrete sex composition and age structure are determined at random.If period has 2 people to occur untoward reaction, then stop test; Result does not find untoward reaction.

4) experimental technique

(1) experimenter's forehead (point left and right two parts) cycle labeling is successively selected: tested region samples group and blank region;

(2) technical staff uses skin oil and fat tester 3 ~ 5 times, SM815 test test position, averages, is designated as initial value.

(3) sample (Pimple removing scream) is smeared at subject position.

(4) experimenter is after making continuously to apply some make up one hour, two hours, four hours, uses skin oil and fat tester by technical staff sM815 measures 3 ~ 5 times, averages.

(5) add up the numerical value recorded, analyze its skin oil and fat changes of contents rule.

5) interpretation of result

Fat content changes

Fat content change is reflected in test period, Experimental Area fat content Changing Pattern in time.Its value is larger, and fat content is larger, otherwise fat content is less.

As shown in Figure 1, sample sets use after 1 hour compared with blank group, diversity is obvious; Sample uses in 4 hours, and the fat content of sample sets is starkly lower than blank group.

Oils and fats increment rate changes

Oils and fats increment rate=(sample sets fat content-initial fat content)/initial fat content

As shown in Figure 2, within test period, recipient site is after sample uses 1 hour, and compared with initial value, skin oil and fat secretion increment rate is starkly lower than blank group, and always lower than blank group.

Press down fat rate

The relative increment rate of oils and fats, namely presses down fat rate=(sample sets fat content-blank group fat content)/blank group fat content; This value is less, illustrates that test specimen presses down fat effect relative to blank sample more obvious.

As shown in Figure 3, within test period, after recipient site uses sample, it presses down fat rate is negative value always.

6) conclusion

After use sample, the fat secretion amount of recipient site is starkly lower than the tested region of not smearing sample, illustrates that product has the ability suppressing skin oil and fat secretion.Inventor utilizes other embodiments to prepare facial cream and repeats above-mentioned experiment, and result is with embodiment 1.

3, prevent pockmark from telling on

1) experimental principle

The pockmark one reparation reaction that to be body produce tissue injury, when the damage of skin is dark and corium or large-area skin blemish, the epidermis at this position can not regenerate, will by corium fabric cell, and the blood vessel of collagen and hypertrophy replaced, and has so just occurred pockmark.Pox scar is because infection inflammation or external force extruding formed, the inflammatory response of Skin Cell cause to the destruction of skin histology and injure corium collagen protein too much, cause the generation of cicatrix.Therefore improve skin environment, keep skin elasticity, ensure that collagen protein is not excessively degraded, the generation of cicatrix can be reduced.

Matrix metallopeptidase 1 (Matrix Metalloproteinases1, MMP-1) is the key enzyme of degrade collagen albumen, suppresses the activity of MMP-1 effectively can reduce the collagen protein amount be degraded, thus reduces the generation of cicatrix.Therefore the effect that the active reaction plant functional component passing through vitro inhibition Fibroblast collagenase (MMP-1) produces cicatrix.

Set up MMP-1 enzyme reaction experiment porch, based on this platform, under the prerequisite of not destructive enzyme reaction system, add plant extract functional component, the change of product fluorescence intensity is detected by enzyme reaction system, the change of indirect reflection enzyme activity, thus reflect that this plant extraction liquid functional component is to the influence of MMP-1 activity.

2) experimental procedure

1. solvent preparation:

(1) buffer (50mmol/L HEPES, 0.12mol/L NaCl, 10mmol/L CaCl2,20 μm of ol/L ZnSO4,0.05%Brij-35, pH=7.5) is prepared,

(2) MMP-1 proenzyme buffer gradient is diluted to 0.22 μ g/100 μ L for subsequent use;

(3) fluorogenic substrate DQ-gelatin buffer gradient is diluted to 0.20 μ g/100 μ L for subsequent use;

(4) in the 0.1mol/L NaOH solution of 1L, add the zymoexcitator APMA of 30mmol, form APMA storing solution (30mmol/L) for subsequent use;

(5) embodiment 1 is prepared sample preparation and become certain density solution for standby;

The activation of 2.MMP-1 proenzyme: MMP-1 proenzyme solution (0.22 μ g/100 μ L) and APMA solution (30mmol/L) are mixed by the volume ratio (MMP:APMA) of 10:1, hatches 45min activation at 37 ° of C;

3. application of sample: get the MMP-1 solution after activation (concentration has reduced to 0.20 μ g/100 μ L) 40 μ L and add in 96 hole ELISA Plate, add embodiment 1 again and prepare Chinese medicine extract 8 μ L, fluorogenic substrate DQ-gelatin solution (0.20 μ g/100 μ L) 52 μ L, make end reaction system be 100 μ L;

Table 7 vitro inhibition MMP-1 tests application of sample table

4. constant-temperature incubation: the 96 hole ELISA Plate completing application of sample are put into 37 DEG C of incubator constant-temperature incubation 600s;

5. experimental result detects

(1). reaction is front is 460nm in excitation wavelength, and emission wavelength is detect the fluorescence intensity treating measuring plants functional component under the testing conditions of 520nm;

(2). reaction system stopping detects the fluorescence intensity of whole reaction system immediately with Bio-Red FLx-800 fluorescence microplate reader after hatching, excitation wavelength is 460nm, and emission wavelength is 520nm.

6. Data Processing in Experiment

MMP-1 suppresses percent (%)=[1-(A1-A2)/Ao] * 100%

The fluorescence intensity of A1-representative sample suppression group

The fluorescence intensity of A2-representative sample self

The fluorescence intensity of Ao-represent blank group

Suppress the active inhibition of percent this kind of plant functional component of larger expression to MMP-1 more remarkable.

3) experimental result

Chinese medicine extract is 98.32% to the maximum inhibition of MMP-1 after tested.It is identical that inventor repeats above-mentioned experimental result with other embodiment preparing products.

Conclusion: Chinese medicine extract of the present invention have prevent pockmark produce and desalination pockmark have positive effect.

4, the organoleptic indicator of anti-acne effect

Choose 100 volunteers, 13 years old-35 years old age, average 21.5 years old, each 50 of men and women.All there is skin lesion in face: pimple, pustule, pockmark.

Using method: after clean, the facial cream sample getting the preparation of appropriate embodiment 1 spreads upon affected part, is massaged into gently and absorbs completely, and twice daily, using for 1 course for the treatment of continuously, 4 weeks is a course for the treatment of.

The standard of curative effect evaluation:

Skin lesion counting × 100% before curative effect=(before treatment the rear skin lesion counting of skin lesion counting-treatment)/treatment

Effective: skin lesion disappears 60% ~ 90%, and symptom alleviates;

Effective: skin lesion disappears 20% ~ 60%, symptom is improved;

Invalid: skin lesion disappears < 20%, and clinical symptoms is not improved;

1) pimple

As shown in Figure 4, after sample uses first week, its action effect for pimple is obvious, and the lasts in 1 ~ 3 three week is good.

2) pustule

As shown in Figure 5, after sample uses 2 weeks, the action effect for pustule of sample is obvious, and has lasting effect.

3) pockmark

As shown in Figure 10, after sample uses 4 weeks, sample prevents the generation action effect of pockmark obvious.

4) conclusion

Volunteer is after being use sample:

(1) from the intuitive visual index of skin: pimple, pustule, pockmark judge, the state that can significantly improve purported skin of its sample sets, the prolongation of the time used with volunteer, it improves effect gradually in increasing trend.

(2) as shown in Fig. 6 to 9, from volunteer self sensation aspect, along with the increase of the time used, its volunteer feels particularly evident, the effect having certain anti-acne, prevent pockmark from producing, wherein cut-off detects last week, and namely the volunteer of 4th week feels that efficiently individual quantity reaches 93%.It is identical that inventor repeats above-mentioned experimental result with other embodiment preparing products.

Claims (8)

1. have a skin care compositions for acne-removing, it is characterized in that, described skin care compositions comprises A phase and B phase, and described A phase is made up of the raw material of following weight percentage ratio:

Described B phase is made up of the raw material of following weight percentage ratio:

Described Chinese medicine extract is obtained through the extraction of field of Chinese medicines general extraction methods by the crude drug of following weight:

Herba Artemisiae Scopariae 20 ~ 30, the Fructus Kochiae 20 ~ 30, Cortex Moutan 10 ~ 20, Galla Chinensis 10 ~ 20, Spina Gleditsiae 10 ~ 20 and Spica Prunellae 10 ~ 20.

2. skin care compositions as claimed in claim 1, is characterized in that, described Chinese medicine extract is obtained through following method extraction by the crude drug of following weight:

Herba Artemisiae Scopariae 20 ~ 30, the Fructus Kochiae 20 ~ 30, Cortex Moutan 10 ~ 20, Galla Chinensis 10 ~ 20, Spina Gleditsiae 10 ~ 20 and Spica Prunellae 10 ~ 20;

The step of described method is as follows:

(1) according to above-mentioned weight weighting raw materials;

(2) percent by volume is 75% ~ 80% ethanol extraction twice, and it is 1:10 ~ 1:20 that first time extracts crude drug and ethanol mass volume ratio g/ml, 60 DEG C ~ 70 DEG C heated and stirred 1 ~ 2 hour, filters to obtain filtrate for the first time; Second time extracts crude drug and ethanol mass volume ratio g/ml is 1:10 ~ 1:20,60 DEG C ~ 70 DEG C heated and stirred 30min ~ 60min, filters to obtain second time filtrate; Filtrate and the mixing of second time filtrate, be concentrated into 0.2 ~ 0.5g/ml, obtain concentrate for the first time;

(3) add propylene glycol or butanediol, sucking filtration according in the mass ratio concentrate that to be 1:1 ~ 1:2 obtain to step (2), 3000 ~ 5000 revs/min of centrifuging and taking supernatant, to obtain final product.

3. skin care compositions as claimed in claim 1 or 2, it is characterized in that, described skin care compositions also comprises C phase, and described C phase is antiseptic 0.05 ~ 0.2wt%.

4. a preparation method for skin care compositions described in claim 1 or 2, is characterized in that, described preparation method comprises the steps:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese medicine extract are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 80 ~ 85 DEG C, mixing;

(2) A phase: weigh each raw material of A phase according to ratio requirement, heat 80 ~ 85 DEG C, mixing;

(3) A phase is all joined in B phase, 2000 ~ 3000 revs/min, homogenizing 5min ~ 10min; 30 ~ 50 revs/min, after stirring 5 ~ 10min, start to stir cooling, speed 30 ~ 50 revs/min, rate of temperature fall is 1 ~ 2 DEG C/min;

(4) when temperature drops to below 45 DEG C, add Chinese medicine extract, stir, continue to be cooled to less than 40 DEG C, to obtain final product.

5. a preparation method for skin care compositions described in claim 3, is characterized in that, described preparation method comprises the steps:

(1) B phase: butanediol and HANSHENGJIAO are stirred, stand-by; Other raw materials of B phase except Chinese medicine extract are added to the water, and add butanediol HANSHENGJIAO mixture after stirring, and heat 80 ~ 85 DEG C, mixing;

(2) A phase: weigh each raw material of A phase according to ratio requirement, heat 80 ~ 85 DEG C, mixing;

(3) A phase is all joined in B phase, 2000 ~ 3000 revs/min, homogenizing 5min ~ 10min; 30 ~ 50 revs/min, after stirring 5 ~ 10min, start to stir cooling, speed 30 ~ 50 revs/min, rate of temperature fall is 1 ~ 2 DEG C/min;

(4) for when temperature drops to below 45 DEG C, add Chinese medicine extract and C phase antiseptic, stir, continue to be cooled to less than 40 DEG C, to obtain final product.

6. have a skin care formulation for acne-removing, it is characterized in that, described skin care formulation is made up of preparation method described in claim 4.

7. have a skin care formulation for acne-removing, it is characterized in that, described skin care formulation is made up of preparation method described in claim 5.

8. the skin care formulation with acne-removing as claimed in claims 6 or 7, it is characterized in that, described skin care formulation is facial cream.