CN110897958A

CN110897958A - Anti-aging composition and application thereof

Info

Publication number: CN110897958A
Application number: CN201911311458.8A
Authority: CN
Inventors: 何备战
Original assignee: Guangzhou And Jia Run Yan Medicine Co Ltd
Current assignee: Guangzhou And Jia Run Yan Medicine Co Ltd
Priority date: 2019-12-18
Filing date: 2019-12-18
Publication date: 2020-03-24

Abstract

The invention belongs to the technical field of cosmetics and discloses an anti-aging composition and application thereof. The anti-aging composition comprises soybean antioxidant active peptide, astragalus polysaccharide and active factors, wherein the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factors is 3-5: 1-3, and the active factors are ethanol extracts obtained by mixing the salvia miltiorrhiza and the ginkgo leaves in mass. The invention also discloses a preparation method of the anti-aging composition and application of the anti-aging composition in cosmetics. The anti-aging composition composed of the soybean antioxidant active peptide, the astragalus polysaccharide and the active factor in a specific ratio has high antioxidant activity and good skin permeability, can effectively lock skin moisture, improve cell metabolism, improve skin microcirculation, promote synthesis of collagen and elastin, enable the skin to be more moist and elastic, and effectively slow down skin aging.

Description

Anti-aging composition and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition and application thereof.

Background

Aging is a spontaneous process of life over time. People in daily life are supposed to age one person through personal appearance, so that each person, especially women, always hopes to seek various methods to resist facial aging and make the aging of the person slower. However, skin aging in most people begins substantially at age 25, and is mainly manifested by increased wrinkles (including frontal lines, transverse lines of the nasal root, longitudinal lines between the eyebrows, crow's feet, and cheek wrinkles, etc.) and sagging skin (including eyebrow sagging, deepening of nasolabial folds, changes in the lower jaw contour, etc.). Facial aging is caused by many reasons, which can be attributed to two factors, internal and external. Firstly, as the skin ages, the regeneration ability of the skin tissue controlled by the gene is weakened, the collagen tissue is reduced, and the skin becomes thinner; second, extrinsic factors such as wind, sun, exhaustion, irregular life, smoking or chronic diseases of the whole body accelerate skin aging. The internal factors are controlled by genes and cannot be regulated manually, but the external factors can be controlled by everyone, which is the reason for the rise of various anti-aging products.

At present, most of anti-aging cosmetics on the market claim to contain various components with high nutritional values, but the anti-aging effect is poor in the actual use process, the anti-aging effect cannot be achieved due to the fact that the anti-aging cosmetics cannot permeate into the interior, most of the anti-aging cosmetics stay on the surface of skin, and only a simple water replenishing and moisturizing effect is achieved. Meanwhile, because the products have high nutritive value and cannot effectively permeate, and the products stay on the surface skin for a long time, various skin problems are very easy to breed. In addition, because various high-nutritive-value components are added, the anti-aging skin care products have higher price, so that people can spend a lot of expenses but cannot obtain the matched care effect. Therefore, if a cosmetic which is effective in anti-aging and low in cost can be developed, great help can be provided for a wide range of anti-aging people.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide an anti-aging composition which is antioxidant and contains active ingredients, and an application thereof, and also provides a preparation method of the anti-aging composition.

The technical scheme adopted by the invention is as follows:

the invention provides an anti-aging composition which comprises soybean antioxidant active peptide, astragalus polysaccharide and an active factor, wherein the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factor is 3-5: 1-3, and the active factor is an ethanol extract obtained by mixing the salvia miltiorrhiza, ginkgo and the like.

Preferably, in the anti-aging composition, the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factor is 5:3: 2.

Preferably, in the anti-aging composition, the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factor is 4:5: 1.

The invention also provides a preparation method of the anti-aging composition, which comprises the step of mixing the soybean antioxidant active peptide, the astragalus polysaccharide and the active factor to obtain the anti-aging composition.

Specifically, the preparation method of the anti-aging composition comprises the following steps: dissolving and modifying soybean protein powder to obtain a mixed solution I, adding papain into the mixed solution I, performing microwave-assisted enzymolysis, performing ultrafiltration concentration, and freeze-drying to obtain the soybean antioxidant active peptide, wherein the mass ratio of the papain to the mixed solution I is 1:500, the enzymolysis time is 40min, the enzymolysis temperature is 53 ℃, the microwave-assisted enzymolysis power is 400W, and the ultrafiltration concentration membrane is 3 kDa.

Specifically, the preparation method of the anti-aging composition comprises the following steps: preparing astragalus powder suspension according to the material-liquid ratio of 1:20, leaching in a water bath at 70 ℃ for 2h, carrying out suction filtration and concentration, standing and centrifuging, and carrying out freeze drying to obtain the astragalus polysaccharide.

The present invention also provides an anti-aging cosmetic comprising the anti-aging composition as described above, in the types including, but not limited to, one or more of skin lotions, milks, essences, gels, creams and masks.

Specifically, when the anti-aging cosmetic is anti-aging skin lotion, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6% of anti-aging composition, 5% of 1, 2-pentanediol, 5% of butanediol, 0.4% of preservative and the balance of deionized water.

Specifically, when the anti-aging cosmetic is anti-aging emulsion, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6-8% of an anti-aging composition, 0.15% of EDTA-2Na, 4% of 1, 3-butanediol, 0.32% of xanthan gum, 3% of hyaluronic acid, 2% of octadecanol, 4% of avocado oil, 2% of polydimethylsiloxane, 2% of caprylic/capric triglyceride, 3% of myristyl myristate, 2% of squalane, 2% of PEG-8 stearate, 1.5% of stearyl alcohol, 0.5% of a preservative, and the balance of deionized water.

Specifically, when the anti-aging cosmetic is anti-aging cream, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6-10% of an anti-aging composition, 0.1% of EDTA-2Na, 4% of 1, 3-butanediol, 94018% of carbomer, 2% of hyaluronic acid, 6% of octadecanol, 6% of avocado oil, 2% of polydimethylsiloxane, 2% of caprylic/capric triglyceride, 2% of myristyl myristate, 2% of squalane, 2% of PEG-8 stearate, 2% of stearyl alcohol, 0.5% of a preservative, and the balance of deionized water.

The invention has the beneficial effects that:

according to the anti-aging composition provided by the invention, the soybean antioxidant active peptide and the astragalus polysaccharide have higher inoxidizability, the molecular weight of the soybean antioxidant active peptide is small, the soybean antioxidant active peptide has good skin permeability, and the combination of the soybean antioxidant active peptide and the astragalus polysaccharide can effectively remove free radicals in cells; meanwhile, the soybean antioxidant active peptide and the astragalus polysaccharide also have good moisturizing performance, can effectively lock skin moisture and improve the metabolism of cells; in addition, the active factors can improve the microcirculation of the skin, assist the anti-aging composition to effectively permeate into the skin, promote the growth of fibroblasts, further promote the synthesis of collagen and elastin, ensure that the skin has better elasticity and effectively slow down aging.

Drawings

FIG. 1 is a graph showing the results of measuring the antioxidant activity of anti-aging compositions provided in test example 1 in various proportions;

FIG. 2 is a graph showing the results of moisture absorption measurements for different proportions of the anti-aging composition provided in test example 1;

FIG. 3 is a graph showing the change in water content of the stratum corneum after using different proportions of the anti-aging cosmetic of the present invention;

FIG. 4 is a graph of skin moisture loss after the use of different proportions of anti-aging cosmetic of the present invention;

FIG. 5 is a graph showing the change in skin elasticity after the use of anti-aging cosmetic according to the present invention in various proportions.

Detailed Description

The invention is further explained by combining the drawings and specific test examples.

Test example 1:

the purpose of this test example was to prepare an anti-aging composition, which was prepared as follows:

1. material

Soybean protein powder (Wuhan flourishing Tianyuan biotechnology, Inc.), radix astragali powder, Saviae Miltiorrhizae radix powder and folium Ginkgo powder (Xian Rui Ying biotechnology, Inc.) are commercially available products.

2. Preparation of soybean antioxidant active peptide

The preparation method of the soybean antioxidant active peptide comprises the following steps:

(1) adding distilled water into soybean protein powder, magnetically stirring for 10min, stirring in 90 deg.C constant temperature water bath for 15min to denature protein, and cooling to room temperature to obtain mixed solution I;

(2) adding papain into the mixed solution I, wherein the mass ratio of the papain to the mixed solution I is 1:500, and uniformly mixing to obtain mixed solution II;

(3) carrying out microwave-assisted enzymolysis on the mixed solution II, and carrying out enzymolysis for 40min at 53 ℃ under the power of 400W to obtain an enzymolysis solution;

(4) inactivating enzyme of the enzymolysis liquid in boiling water bath for 10min, cooling, and centrifuging to obtain supernatant;

(5) ultrafiltering and concentrating the supernatant, filtering with a 3kDa filter membrane, and collecting the concentrated solution;

(6) freeze drying the concentrated solution to obtain the required soybean antioxidant active peptide.

3. Preparation of Astragalus polysaccharides

Adding radix astragali powder into deionized water, wherein the mass ratio of the radix astragali powder to the deionized water is 1:20, obtaining radix astragali powder suspension, leaching in a thermostatic water bath at 70 ℃ for 2h, carrying out suction filtration and concentration to 1/5 of stock solution, slowly adding 95% ethanol into the concentrated solution until the ethanol content is 80% (V/V), placing the solution in a refrigerator for 12-16 h, centrifuging at 3000r/min for 15min, washing filter residues with absolute ethyl alcohol, acetone and diethyl ether respectively to obtain loose powder, adding appropriate amount of water for redissolving, and carrying out freeze drying to obtain astragalus polysaccharide.

4. Preparation of active factor

Weighing a traditional Chinese medicine mixture (mixed by the weight of salvia miltiorrhiza powder and ginkgo leaf powder and the like), adding the mixture into deionized water according to the material-liquid ratio of 1:30, and performing reflux extraction by using ethanol, wherein the extraction temperature is 70 ℃, the extraction time is 50min, and the ultrasonic power is 350W; after ultrasonic extraction, the extract was concentrated using a rotary evaporator, evaporated to dryness in a 45 ℃ water bath, and dried at 50 ℃ to obtain the active factor (powder).

5. Preparation of anti-aging composition

And (3) uniformly mixing the soybean antioxidant active peptide, the astragalus polysaccharide and the active factor according to the table 1 to obtain the anti-aging composition.

TABLE 1 antiaging compositions in different proportions

Grouping	Mass ratio of
		1	The soybean antioxidant active peptide comprises astragalus polysaccharide and active factors in a ratio of 3:5:2
2	The soybean antioxidant active peptide comprises astragalus polysaccharide and active factors of 4:4:2
		3	Soybean antioxidant active peptideAstragalus polysaccharide and active factors are 4:3
4	The soybean antioxidant active peptide comprises astragalus polysaccharide and active factors of 5:3:2
		5	The soybean antioxidant active peptide comprises astragalus polysaccharide and active factors of 5:4:1
6	The soybean antioxidant active peptide comprises astragalus polysaccharide and active factors of 4:5:1

6. Measurement of Performance

6.1DPPH free radical scavenging Activity assay

Preparing anti-aging compositions with different proportions into 5mg/mL solution to be detected, sucking 2.0mL of the solution to be detected, adding 2.0mL of DPPH solution (solvent is absolute ethyl alcohol), mixing, reacting at 25 ℃ in a dark place for 1h, and detecting the absorbance Ai at 517 nm; the control group is 2.0mL of ethanol solution and 2.0mL of sample, and the absorbance is measured to be Aj; the blank was 2.0mL ethanol solution and 2.0mL DPPH.solution.

6.2 measurement of moisture Retention Properties

Placing the anti-aging compositions with water content of 10% in different drying bottles (dry silica gel is placed in the drying bottles in advance). And respectively weighing after placing, comparing the moisture retention performance of the anti-aging compositions with different proportions, and calculating the residual moisture rate. Water remaining ratio (%) ═ H_n/H_o×100％，H_nIs the mass of the sample after placement; h_oIs the mass of the sample before placement.

The results are shown in fig. 1, and among the anti-aging compositions in different proportions, the anti-aging composition shown in group 4 exhibited the highest DPPH radical scavenging rate and the best anti-oxidant properties. Moisturizing performance measurements the results of the moisturizing performance measurements are shown in figure 2, with the anti-aging compositions shown in

groups

4 and 6 having better moisturizing performance than

groups

2 and 3 among the different groups of anti-aging compositions. It can be seen that the anti-aging composition shown in group 4 is optimized for use in cosmetics, but it is understood that the anti-aging compositions shown in groups 1-3 and

groups

5 and 6 can be used in anti-aging cosmetic preparations.

The anti-aging composition provided by the invention can be used for preparing various types of cosmetics, including but not limited to skin lotion, emulsion, essence, cream and facial mask. When used for preparing the cosmetic, the composition contains one or more of the conventional cosmetic matrix components such as emulsifier, oil, emollient, humectant, thickener, essence, and antiseptic, in addition to the above antiaging composition.

Test example 2

The purpose of this test example is to provide an anti-aging skin lotion.

(1) Weighing deionized water and propylene glycol according to the weight shown in the table 2, adding the anti-aging composition (with the mass fraction of 2%, 4%, 6%, 8% or 10%) of the group 4 in the test example 1, and uniformly mixing to obtain a first mixture;

(2) mixing glycerol and antiseptic to obtain mixture II;

(3) adding the mixture II into the mixture I, and uniformly mixing to obtain a mixture III;

(4) and stirring and cooling the mixture III, and discharging and filling.

TABLE 2 formula of antiaging skin lotion

Components	Mass fraction/%
		Anti-aging composition	2. 4, 6, 8 or 10
Butanediol	5
		1, 2-pentanediol	5
Preservative	0.4
		Deionized water	Balance of

Test example 3

The purpose of this test example is to provide an antiaging emulsion.

(1) Weighing phase A in sequence as shown in Table 3, sterilizing in boiling water for 15min, and supplementing evaporated water;

(2) weighing the phase B components in sequence as shown in Table 3, and dissolving in boiling water for 15 min;

(3) after the two phases are fully dissolved, cooling to 80 ℃, slowly pouring the phase B into the phase A, homogenizing at 4000r/min for 5min, cooling to 45 ℃, and adding a preservative and the anti-aging composition (with the mass fraction of 2%, 4%, 6%, 8% or 10%) described in group 4 of experimental example 1;

(4) vacuumizing to remove bubbles, stirring and cooling, and discharging and filling.

TABLE 3 anti-aging emulsion formulas

Test example 4

The purpose of this test example is to provide an anti-aging cream.

(1) Weighing phase A in sequence as shown in Table 4, sterilizing in boiling water for 15min, and supplementing evaporated water;

(2) weighing the phase B components in sequence as shown in Table 4, and dissolving in boiling water for 15 min;

(4) stirring and cooling, and discharging and filling.

TABLE 4 anti-aging cream formula

Test example 5

The purpose of this test example is to test the performance of the anti-aging cosmetic of test examples 2 to 4.

In this test example, the performance of the anti-aging cosmetic was judged by testing the water content of the stratum corneum, the skin water loss (TEWL), and the skin elasticity of the skin of a subject (25-30 years old, female, 13 people). Different testees use the same position on the inner side of the arm on the same side as a test part (each testee has 5 marked positions on the arm on the same side for testing 5 concentrations), the test part is not coated with any cosmetics within 1 month before the test, and after the test is started, the testees coat the test part on the inner side of the arm with the skin lotion, the emulsion and the cream (sequentially coated) once every morning and evening. Cleaning with clear water before each measurement, and measuring after 20 min.

The stratum corneum water content, the skin water loss (TEWL), and the change in skin elasticity are shown in fig. 3 to 5, respectively. As can be seen from FIG. 3, as the concentration increases, the moisture content of the horny layer increases more as the cosmetic is applied for a longer time, and the moisture content of the horny layer increases more than that of the other concentrations when the anti-aging composition is contained in the cosmetic at 6% to 8%, which is optimal at 8%; as can be seen from fig. 4, when the content of the antiaging composition in the antiaging cosmetic was 6%, the change rate of TWEL was the lowest, that is, the score of TWEL was the lowest, indicating that the effect of protecting the moisture content of the keratin layer using the antiaging cosmetic at this concentration was superior to that of the other groups; as can be seen from fig. 5, using cosmetics containing 6% to 10% of the anti-aging composition, the skin elasticity was significantly better than that of anti-aging cosmetics of 2% and 4% concentration, indicating that the anti-wrinkle performance of the anti-aging cosmetics was best at 6% to 10%. In view of the above results and economic cost, the amount of 6% added is preferable in practical production.

The present invention is not limited to the above-described alternative embodiments, and various other forms of products can be obtained by anyone in light of the present invention. The above detailed description should not be taken as limiting the scope of the invention, which is defined in the claims, and which the description is intended to be interpreted accordingly.

Claims

1. An anti-aging composition characterized by: the composition comprises soybean antioxidant active peptide, astragalus polysaccharide and active factors, wherein the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factors is 3-5: 1-3, and the active factors are ethanol extracts obtained by mixing the salvia miltiorrhiza, the ginkgo and the like.

2. The anti-aging composition according to claim 1, characterized in that: the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factors is 5:3: 2.

3. The anti-aging composition according to claim 1, characterized in that: the mass ratio of the soybean antioxidant active peptide to the astragalus polysaccharide to the active factors is 4:5: 1.

4. A method of preparing the antiaging composition according to any one of claims 1 to 3, characterized in that: and mixing the soybean antioxidant active peptide, the astragalus polysaccharide and the active factors to obtain the anti-aging composition.

5. The method of preparing the anti-aging composition according to claim 4, wherein the soybean antioxidant active peptide is prepared by the steps of: dissolving and modifying soybean protein powder to obtain a mixed solution I, adding papain into the mixed solution I, performing microwave-assisted enzymolysis, performing ultrafiltration concentration, and freeze-drying to obtain the soybean antioxidant active peptide, wherein the mass ratio of the papain to the mixed solution I is 1:500, the enzymolysis time is 40min, the enzymolysis temperature is 53 ℃, the microwave-assisted enzymolysis power is 400W, and the ultrafiltration concentration membrane is 3 kDa.

6. The method for preparing the antiaging composition according to claim 4 or 5, characterized in that the astragalus polysaccharides are prepared by the following steps: preparing astragalus powder suspension according to the material-liquid ratio of 1:20, leaching in a water bath at 70 ℃ for 2h, carrying out suction filtration and concentration, standing and centrifuging, and carrying out freeze drying to obtain the astragalus polysaccharide.

7. An anti-aging cosmetic characterized by: the antiaging cosmetic comprises the antiaging composition as set forth in any one of claims 1 to 3, and the types of the antiaging cosmetic comprise skin lotion, emulsion, essence, gel, cream and/or pack.

8. The anti-aging cosmetic according to claim 7, wherein when the anti-aging cosmetic is anti-aging lotion, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6% of anti-aging composition, 5% of 1, 2-pentanediol, 5% of butanediol, 0.4% of preservative and the balance of deionized water.

9. The anti-aging cosmetic according to claim 7, wherein when the anti-aging cosmetic is an anti-aging emulsion, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6-8% of an anti-aging composition, 0.15% of EDTA-2Na, 4% of 1, 3-butanediol, 0.32% of xanthan gum, 3% of hyaluronic acid, 2% of octadecanol, 4% of avocado oil, 2% of polydimethylsiloxane, 2% of caprylic/capric triglyceride, 3% of myristyl myristate, 2% of squalane, 2% of PEG-8 stearate, 1.5% of stearyl alcohol, 0.5% of a preservative, and the balance of deionized water.

10. The anti-aging cosmetic according to claim 7, wherein when the anti-aging cosmetic is an anti-aging cream, the anti-aging cosmetic is mainly prepared from the following raw materials in percentage by weight: 6-10% of an anti-aging composition, 0.1% of EDTA-2Na, 4% of 1, 3-butanediol, 94018% of carbomer, 2% of hyaluronic acid, 6% of octadecanol, 6% of avocado oil, 2% of polydimethylsiloxane, 2% of caprylic/capric triglyceride, 2% of myristyl myristate, 2% of squalane, 2% of PEG-8 stearate, 2% of stearyl alcohol, 0.5% of a preservative, and the balance of deionized water.