CN114712269A

CN114712269A - Anti-aging essential oil composition and preparation method and application thereof

Info

Publication number: CN114712269A
Application number: CN202210146152.7A
Authority: CN
Inventors: 孙怀庆; 王开慧; 孙云起; 郭朝万; 陈杰
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-02-17
Filing date: 2022-02-17
Publication date: 2022-07-08

Abstract

The invention discloses an anti-aging essential oil composition, a preparation method and an application thereof, wherein the composition comprises the following components: an oil phase, an emulsifier, an excipient and a water phase; wherein the oil phase comprises the following raw materials: camellia seed oil, frankincense essential oil and sandalwood essential oil; the emulsifier is a solution of chitosan and guar gum; the aqueous phase comprises water and a skin conditioning agent comprising: salmon roe extract, peach fruit extract and corn gluten amino acids. The composition has effects of tightening, removing wrinkle and resisting aging, and the specific emulsifier is selected, so that the stability of the composition can be improved, and the retention rate of effective components can be improved. The composition is prepared by preparing emulsion by an emulsification method and then further preparing the emulsion by a freeze drying technology, so that the stability of the product is improved, and the storage life of the product is prolonged. The composition can be used as one of raw materials, is applied to facial masks, essence, toner and cream, and has the advantages of remarkable anti-wrinkle and anti-aging effects, safety in use and wide application range.

Description

Anti-aging essential oil composition and preparation method and application thereof

Technical Field

The invention relates to the technical field of anti-aging skin care products, and particularly relates to an anti-aging essential oil composition and a preparation method and application thereof.

Background

With the influence of organism aging and photoaging, the horny cells at the outermost layer of epidermis are sunken, the network structure of collagen elastic fibers in the dermis structure is degenerated, and the macroscopic appearance of skin is wrinkles. The addition of functional components in the beauty products has become an important principle for the design of anti-aging beauty products.

The anti-wrinkle product in the market at present mainly comprises 1) antioxidant enzyme such as SOD, but the enzymes have poor stability, and are basically inactivated after being placed for a long time at any time; 2) small-molecular antioxidant substances such as vitamin C, E and the like, but the substances only have antioxidant effect and can prevent wrinkles, but cannot remove the generated wrinkles, and the effect is not satisfactory; 3) the natural extracts, such as ginseng, rhodiola rosea and the like, have high cost and low transdermal absorption rate, and do not really reach the basal layer of the skin to exert the effect obviously. The existing anti-wrinkle product with traditional Chinese medicine extract is more suitable for preventing wrinkles and is not suitable for the wrinkles which are generated. 4) Some products are hormone substances, and some products are often excessively added with some hormone substances for quick effect, so that the potential damage to the skin is large. In addition to the use of these cosmetic products for external use, there are also cosmetic and cosmetic procedures in beauty parlors for wrinkle removal, such as subcutaneous injections of hyaluronic acid, botulism, etc., which are, on the one hand, costly and questionable as to the safety, such as, for example, the insertion of the filler into the subcutaneous tissue by surgery, the retention thereof in the body for a long time, with a certain safety risk, and, on the other hand, injections often require frequent injections.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide an anti-aging essential oil composition; the second purpose of the invention is to provide a preparation method of the anti-aging essential oil composition, the composition is prepared into solid freeze-dried powder by adopting a freeze-drying method, the loss of effective components is avoided, the storage is convenient, and the shelf life is prolonged; the invention also aims to provide the application of the anti-aging essential oil composition, which can be used as a raw material of an anti-wrinkle skin care product, is wide in application and convenient to store.

One of the purposes of the invention is realized by adopting the following technical scheme:

an anti-aging essential oil composition, comprising the following components: an oil phase, an emulsifier, an excipient and a water phase; wherein the oil phase comprises the following raw materials: camellia seed oil, frankincense essential oil and sandalwood essential oil; the emulsifier is a solution of chitosan and guar gum; the aqueous phase comprises water and a skin conditioning agent comprising: salmon roe extract, peach fruit extract and corn gluten amino acids.

Specifically, the efficacy of each component is as follows:

and (3) camellia seed oil: the tea seed oil, tea tree oil and tea oil belong to non-drying oil, are woody plant oil which is extracted from tea tree seeds and is rich in unsaturated fatty acid, have clear color, delicious taste and fragrant flavor, slightly contain a certain amount of saponin and multiple vitamins, have the functions of sterilization and detoxification, and the refined tea oil has good thermal stability and is not easy to stably deteriorate. 70-80% of the camellia seed oil is the same as human skin, has good affinity with the skin, is easy to be absorbed by the skin, can maintain skin cells and normal physiological functions and metabolic activities, is used for skin care products, can promote the skin to be tender and elastic, and has good skin moistening and oxidation resisting functions.

Frankincense essential oil: extracted from mastic resin, emits warm and pure wood fragrance, and also emits light fruity fragrance, which can make people feel relaxed and relaxed never before. Frankincense is used for making facial masks to keep young as early as ancient egypt. The essential oil is light yellow, and has antibacterial, wound scar promoting, scar and wrinkle removing, cell activity enhancing, tranquilizing, body tonifying, and rejuvenating effects, and can be used for regulating dry, aged and dark skin, recovering skin elasticity, and tightening pore.

Sandalwood essential oil: has effects of promoting skin cell growth, rapidly recovering wound or scar, and further has elasticity and contraction effects, and can balance and soften skin, improve dryness and weaken texture, and is especially suitable for caring skin with aging, dryness and water deficiency, and neck. Can be used for treating dry skin, hardened cutin, xerotic eczema, and wound. It also has antibacterial effect, and can relieve skin itching and inflammation, and improve pimple, furuncle and infected wound.

Salmon roe extract: is a precious natural extract. Has the effects of strongly removing fine lines and wrinkles, reducing color spots and red blood filaments, brightening skin color and keeping skin moisture. It also contains eicosapentaenoic acid which can effectively reduce skin red blood silk, and docosahexaenoic acid which can reduce the oxidative stress of skin cells, so that the synthesis amount of collagen is increased, the astaxanthin antioxidation effect is obvious, and the skin aging can be inhibited. It also contains astaxanthin, and has antioxidant, elastin synthesis promoting, and anti-inflammatory effects. Also contains vitamin A and vitamin E, and has effects of protecting against light injury and reducing fine lines and wrinkles.

Peach fruit extract: contains abundant fruit acid, and has effects of keeping moisture, removing dirt in pore, preventing pigmentation, and improving pachylosis. The peach fruit extract has the proliferation effect on epidermal keratinocytes, inhibits the generation of nitrogen oxides, has oxygen resistance and can obviously improve the water content of the horny layer, so that the peach fruit extract has good skin-activating and anti-aging effects and can be used for skin aging caused by dry skin; actin-myosin is a model simulating the structure of the dermis of the skin, and peach fruit extract has a contraction effect on the actin-myosin, which can indicate that the actin-myosin has a skin tightening effect.

Further, the water phase also comprises heparin, and the mass ratio of the skin conditioner to the heparin is 100: (0.1-0.5).

Still further, the excipient is one or a combination of more than two of glycine, sodium dihydrogen phosphate, disodium hydrogen phosphate, mannitol and carboxymethyl chitosan.

Further, the composition comprises the following components in percentage by mass: 90-95% of a water phase, 0.1-0.3% of an oil phase, 0.1-0.5% of an emulsifier and 5-15% of an excipient; wherein the mass ratio of the skin conditioner to water in the water phase is (0.05-3): 100.

further, the emulsifier is formed by mixing chitosan contained in a chitosan acetic acid solution and a guar gum solution; wherein the chitosan-acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: (2-3).

Further, the composition comprises the following components in parts by mass:

oil phase: 6-10% of camellia seed oil, 0.01-0.05% of frankincense essential oil and 0.01-0.05% of sandalwood essential oil;

emulsifier: 0.01-0.03% of chitosan and guar gum solution;

excipient: 0.1-0.3% of glycine, 0.4-0.5% of sodium dihydrogen phosphate, 0.1-0.2% of disodium hydrogen phosphate, 0.3-0.5% of mannitol and 0.5-1% of carboxymethyl chitosan;

water phase: 0.5-3% of salmon roe extract, 0.5-3% of peach fruit extract, 0.5-3% of corn gluten amino acids and the balance of water.

The second purpose of the invention is realized by adopting the following technical scheme:

the preparation method of the anti-aging essential oil composition comprises the following steps:

1) mixing the components of the emulsifier and the oil phase to obtain a mixture;

2) mixing the mixture and the water phase to obtain mixed emulsion;

3) mixing the mixed emulsion and an excipient to obtain a mother solution;

4) and (4) freeze-drying the mother liquor to obtain the freeze-dried powder anti-aging essential oil composition.

Further, in the step 1), before the emulsifier is mixed with the oil phase, the pH value is adjusted to 3-5. The chitosan solution has positive charges, the guar gum solution has negative charges, the electrostatic interaction of the chitosan solution and the guar gum solution is strongest, and the emulsification effect is best.

Further, in the step 2), in the step 3), the pH value of the mother liquor is adjusted to 4-6.

The third purpose of the invention is realized by adopting the following technical scheme:

the application of the anti-aging essential oil composition is used for preparing cosmetics.

Compared with the prior art, the invention has the beneficial effects that:

(1) the composition of the present invention comprises the following ingredients: an oil phase, an emulsifier, an excipient and a water phase; wherein the oil phase comprises the following raw materials: camellia seed oil, frankincense essential oil and sandalwood essential oil; the emulsifier is a solution of chitosan and guar gum; the aqueous phase comprises water and a skin conditioning agent comprising: salmon roe extract, peach fruit extract and corn gluten amino acids. The camellia oil has good skin moistening effect and antioxidant effect, the frankincense essential oil and the sandalwood essential oil are used as auxiliary materials, the tightening, wrinkle removing and anti-aging effects are achieved, the salmon roe extract is compounded with the peach fruit extract and the corn gluten amino acids, the peach fruit extract has a synergistic effect on the anti-aging effect of the salmon roe extract, and the better anti-aging effect can be achieved under the condition that the addition amount of the noble raw material salmon roe extract is low. The solution of chitosan and guar gum is used as an emulsifier, so that the stability of the composition can be improved, and the retention rate of active ingredients can be improved.

(2) After the composition is redissolved to form a solution, the composition is easy to oxidize due to oxidation resistance, so that the liquidity is weakened, the solidification speed is high, and the using effect of the product is reduced.

(3) The preparation method of the composition comprises the steps of preparing the emulsion by an emulsification method, and then preparing the emulsion into the solid freeze-dried powder by a freeze-drying technology, so that the change of the physicochemical and biological characteristics of the product is effectively prevented, the damage to the structure and the characteristics of biological tissues and cells is small, the product rapidly enters a dormant state, and the stability of the effective components of a plurality of thermosensitive medicinal biological products is effectively protected. Such as astaxanthin, will not denature and lose its biological activity; secondly, the freeze-dried product is loose in shape and basically not changed in color after being dried, can be quickly dissolved and recover the physical and chemical properties and biological activity of the original aqueous solution after being added with water, and is high in redissolution speed. Thirdly, because the drying is carried out under the vacuum condition, the protective effect on some substances which are easy to oxidize is good. Fourthly, the moisture content of the freeze-dried product is very low, so that the stability of the product is improved, the chance of pollution is reduced, the transportation is convenient, and the storage life of the product is prolonged.

(4) The composition can be used as one of raw materials, is applied to masks, essence, toning water and creams, and has the advantages of obvious anti-wrinkle and anti-aging effects, safety in use and wide application range.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.

Example 1

The anti-aging essential oil composition comprises the following components in parts by mass:

oil phase: camellia seed oil 6%, frankincense essential oil 0.01%, and sandalwood essential oil 0.01%;

emulsifier: 0.01% of chitosan and guar gum solution; wherein the chitosan-acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: 2;

excipient: 0.1% of glycine, 0.4% of sodium dihydrogen phosphate, 0.1% of disodium hydrogen phosphate, 0.3% of mannitol and 0.5% of carboxymethyl chitosan;

water phase: 0.5% of salmon roe extract, 0.5% of peach fruit extract, 0.5% of corn gluten amino acids and the balance of deionized water.

1) adjusting the pH value of the emulsifier to 3, and then mixing and stirring the emulsifier and the components of the oil phase to obtain a mixture;

2) mixing the mixture and the water phase to obtain mixed emulsion;

3) mixing the mixed emulsion and an excipient to obtain a mother solution, and adjusting the pH of the mother solution to 4;

Example 2

oil phase: 10% of camellia seed oil, 0.05% of frankincense essential oil and 0.05% of sandalwood essential oil;

emulsifier: 0.03% of chitosan and guar gum solution; wherein the chitosan-acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: 3;

excipient: 0.3% of glycine, 0.5% of sodium dihydrogen phosphate, 0.2% of disodium hydrogen phosphate, 0.5% of mannitol and 1% of carboxymethyl chitosan;

water phase: 3% of salmon roe extract, 3% of peach fruit extract, 3% of corn gluten amino acids and the balance of deionized water.

1) adjusting the pH value of the emulsifier to 5, and then mixing and stirring the emulsifier and the components of the oil phase to obtain a mixture;

2) mixing the mixture and the water phase to obtain mixed emulsion;

3) mixing the mixed emulsion and an excipient to obtain a mother solution, and adjusting the pH of the mother solution to 6;

Example 3

oil phase: camellia seed oil 8%, frankincense essential oil 0.02%, and sandalwood essential oil 0.03%;

emulsifier: 0.02% of a solution of chitosan and guar gum; wherein the chitosan acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: 2.5;

excipient: 0.2% of glycine, 0.45% of sodium dihydrogen phosphate, 0.15% of disodium hydrogen phosphate, 0.4% of mannitol and 0.8% of carboxymethyl chitosan;

water phase: salmon roe extract 2%, peach fruit extract 2%, corn gluten amino acids 1.5% and heparin 0.055%; the balance being deionized water.

2) mixing the mixture and the water phase to obtain mixed emulsion;

3) mixing the mixed emulsion and an excipient to obtain a mother solution, and adjusting the pH of the mother solution to 5;

Example 4

oil phase: camellia seed oil 7%, frankincense essential oil 0.01%, and sandalwood essential oil 0.02%;

excipient: 0.1% of glycine, 0.4% of sodium dihydrogen phosphate, 0.2% of disodium hydrogen phosphate, 0.45% of mannitol and 0.6% of carboxymethyl chitosan;

water phase: 1% of salmon roe extract, 1.5% of peach fruit extract, 2% of corn gluten amino acids and 0.036% of heparin, and the balance of deionized water.

1) adjusting the pH value of the emulsifier to 4, and then mixing and stirring the emulsifier and the components of the oil phase to obtain a mixture;

2) mixing the mixture and the water phase to obtain mixed emulsion;

Example 5

oil phase: 10% of camellia seed oil, 0.03% of frankincense essential oil and 0.015% of sandalwood essential oil;

emulsifier: 0.012% solution of chitosan and guar gum; wherein the chitosan-acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: (2-3);

excipient: 0.2% of glycine, 0.4% of sodium dihydrogen phosphate, 0.1% of disodium hydrogen phosphate, 0.35% of mannitol and 0.7% of carboxymethyl chitosan;

water phase: salmon roe extract 2%, peach fruit extract 1%, corn gluten amino acids 0.7%, and heparin 0.11%, and the balance deionized water.

2) mixing the mixture and the water phase to obtain mixed emulsion;

Comparative example 1

Comparative example 1 differs from example 3 in that: comparative example 1 the salmon roe extract was not added and the amount was made up with purified water, and the remaining components and preparation method were the same as in example 3.

Comparative example 2

Comparative example 2 differs from example 3 in that: comparative example 2 no peach fruit extract was added, the amount was made up with purified water, and the remaining components and preparation method were the same as in example 3.

Comparative example 3

Comparative example 3 differs from example 3 in that: comparative example 3 no corn gluten amino acids were added, the amount was made up with purified water, and the remaining ingredients and preparation method were the same as in example 3.

Comparative example 4

Comparative example 4 differs from example 3 in that: comparative example 4 the emulsifier was replaced with ceteareth-30 and the remaining components and preparation were the same as in example 3.

Comparative example 5

Comparative example 5 differs from example 3 in that: comparative example 5 camellia seed oil was replaced with glycerol, and the remaining components and preparation method were the same as in example 3.

Comparative example 6

Comparative example 6 differs from example 3 in that: comparative example 6 the boswellia essential oil was replaced with glycerin, and the remaining components and preparation method were the same as in example 3.

Comparative example 7

Comparative example 7 differs from example 3 in that: comparative example 7 sandalwood essential oil was replaced with glycerin, and the remaining components and preparation method were the same as in example 3.

Comparative example 8

Comparative example 8 differs from example 3 in that: comparative example 8 no excipient was added and freeze-drying was not performed, and the final finished state was an emulsion.

Performance test

First, in vitro DPPH free radical scavenging experiment

Preparation of DPPH free radical solution: weighing a certain amount of DPPH free radical powder, dissolving with absolute ethyl alcohol, fixing the volume to 500mL, and preparing into a solution with the concentration of 2 x 10^-3mol/L, as mother liquor (stored in dark place), diluted to a concentration of 0.8X 10^-4The initial reaction concentration of the DPPH solution is mol/L.

Preparing a sample solution: weighing 1g of the freeze-dried powder of examples 1-5 and comparative examples 1-7 and the emulsion of comparative example 8, and adding the weighed freeze-dried powder and emulsion into 20mL of pure water to obtain 14 groups of sample solutions respectively.

The test method comprises the following steps: taking 2mL of sample solution and 2.5mL of LDPPH free radical solution As sample groups, adding the sample groups into a 5mL centrifuge tube, shaking up, sealing and standing for 20min at room temperature, and measuring the absorbance value at the wavelength of 517nm and recording As; taking a mixed solution of X mL of blank solvent and 2.5mL of DPPH free radical as a control group, and measuring the absorbance value as Ac; taking a mixed solution of X mL of sample and 2.5mL of absolute ethyl alcohol as a blank set, and measuring the absorbance value as Ab; the volume of the solution was adjusted to zero with 50% ethanol, and ascorbic acid (Vc) was used as a positive control.

The DPPH free radical removing capacity of the sample is calculated according to a formula (1), and the in-vitro antioxidant capacity of the sample is inspected by taking the DPPH free radical removing capacity as an index.

In the formula: as: absorbance of (sample solution + DPPH radical solution); ac: absorbance of (sample solvent + DPPH radical solution); ab: (sample solution + absolute ethanol solution).

Measurement and calculation of DPPH free radical clearance rate of each group: respectively preparing mother liquor of each medicine extract and 0.2g/L mother liquor of ascorbic acid (Vc). Diluting the mother liquor of each medicine into a series of concentrations, and measuring the DPPH free radical clearance of samples with each concentration. Specific data are shown in table 1.

TABLE 1 DPPH radical scavenging Rate for each set of sample solutions

As can be seen from Table 1, the effect of eliminating free radicals is not much affected by adding no excipient (comparative example 8) and replacing the emulsifier with ceteareth-30 (comparative example 4), and the anti-aging effect of salmon roe extract, peach fruit extract, corn gluten amino acids, camellia oil, frankincense essential oil and sandalwood essential oil is synergistic as can be seen from comparative examples 1-3 and comparative examples 5-7 in comparison with examples 1-5. Among them, example 3 is the most preferable example.

Second, sample stability test

Since only comparative example 4 and comparative example 8 are comparative examples of non-efficacy ingredients, the subjects of the test were examples 1 to 5 and comparative examples 4 and 8.

The test method comprises the following steps: the freeze-dried powder products of examples 1-5 and comparative example 4 and the emulsion of comparative example 8 were stored in a sealed and dark environment at room temperature (25 + -2 ℃), taken out at 0 and 30 days, respectively, and the stability of the sample was examined using the particle size and encapsulation efficiency after redispersion as indices. The results are shown in Table 2.

Method for measuring average particle diameter: preparing each group of samples into suspension, filtering the suspension with 0.3 μm lipid membrane, diluting with ultrapure water, placing in ultrasonic cleaning instrument, oscillating for 1min to disperse particles uniformly, and measuring with Malvern particle size analyzer.

The method for measuring the encapsulation efficiency comprises the following steps: the content of astaxanthin in the salmon roe extract is used as a test index, the content of astaxanthin in the freeze-dried powder is determined by adopting a three-dimensional fluorescence spectrum, and the encapsulation efficiency is determined by adopting a glucan gel filtration method. Placing 5.0G Sephadex G-50 (swollen with water) for more than 12h in a (1cm × 20cm) gel column, pre-saturating the gel column with blank lipid nanoparticles, and precisely sucking sample suspension 0.2mL (V)_{General assembly}mL) was loaded onto the column and eluted with deionized water as the mobile phase at a flow rate of 0.5mL min-1, one tube per 1 mL. And measuring the mass fraction of astaxanthin in the fraction.

The mass of astaxanthin encapsulated in the suspension was determined separately (W)_CarrierG) mass of free astaxanthin (W)_{Swimming device}G). Simultaneously taking 0.2mL of sample suspension, adding 0.2mL of TritonX-100 ethanol solution for complete demulsification, diluting to constant volume, and determining the total mass (W) of astaxanthin_{General assembly}G). Calculating the package of lyophilized powder according to the following formulaSealing rate (Q)_{Bag (bag)})。

Q_{Bag (bag)}(％)＝(W_{General assembly}-W_{Swimming device})/W_{General assembly}×100％ (2)

As shown in Table 2, examples 3 to 5 have higher encapsulation efficiency and smaller average particle size change, because heparin is added in examples 3 to 5, the heparin can prolong the fluidity of the solution in which the lyophilized powder is redissolved, and shorten the coagulation time. While comparative example 4, in which the emulsifier was replaced with cetostearyl polyether-30, the emulsifying effect was inferior to that of the solution of chitosan and guar gum, indicating that the emulsifier of the present invention can improve the encapsulation efficiency and stability. The emulsion of comparative example 8 was phase-separated after 30 days of storage at room temperature, and it was found that stability was improved by adding excipients and performing a freeze-drying operation.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. An anti-aging essential oil composition, which is characterized by comprising the following components: an oil phase, an emulsifier, an excipient and a water phase; wherein the oil phase comprises the following raw materials: camellia seed oil, frankincense essential oil and sandalwood essential oil; the emulsifier is a solution of chitosan and guar gum; the aqueous phase comprises water and a skin conditioning agent comprising: salmon roe extract, peach fruit extract and corn gluten amino acids.

2. The anti-aging essential oil composition of claim 1, wherein the aqueous phase further comprises heparin, and the mass ratio of the skin conditioner to the heparin is 100: (0.1-0.5).

3. The anti-aging essential oil composition of claim 1, wherein the excipient is one or more of glycine, sodium dihydrogen phosphate, disodium hydrogen phosphate, mannitol, and carboxymethyl chitosan.

4. The anti-aging essential oil composition of claim 1, wherein the composition comprises the following components in percentage by mass: 90-95% of a water phase, 0.1-0.3% of an oil phase, 0.1-0.5% of an emulsifier and 5-15% of an excipient; wherein the mass ratio of the skin conditioner in the water phase to the water is (0.05-3): 100.

5. the anti-aging essential oil composition of claim 1, wherein the emulsifier is a mixture of chitosan contained in a chitosan acetic acid solution and a guar gum solution; wherein the chitosan-acetic acid solution contains chitosan and guar gum in the guar gum solution in a mass ratio of 1: (2-3).

6. The anti-aging essential oil composition of claim 1, wherein the composition comprises the following ingredients in parts by mass:

emulsifier: 0.01-0.03% of chitosan and guar gum solution;

7. The method for preparing an anti-aging essential oil composition as claimed in any one of claims 1 to 6, which comprises the steps of:

2) mixing the mixture and the water phase to obtain mixed emulsion;

3) mixing the mixed emulsion and an excipient to obtain a mother solution;

8. The method for preparing an anti-aging essential oil composition according to claim 7, wherein the pH is adjusted to 3 to 5 before the emulsifier is mixed with the oil phase in step 1).

9. The method for preparing an anti-aging essential oil composition as claimed in claim 7, wherein in the step 2), the pH of the mother liquor is adjusted to 4-6 in the step 3).

10. Use of an anti-ageing essential oil composition according to any one of claims 1 to 8, for the preparation of a cosmetic product.