CN114748385B

CN114748385B - Composition and eye cream with effects of resisting wrinkles, removing dark eye circles and improving eye bags

Info

Publication number: CN114748385B
Application number: CN202210367393.4A
Authority: CN
Inventors: 李占鸿
Original assignee: Guangzhou Unes Biotechnology Co ltd
Current assignee: Guangzhou Unes Biotechnology Co ltd
Priority date: 2022-04-08
Filing date: 2022-04-08
Publication date: 2022-12-09
Anticipated expiration: 2042-04-08
Also published as: CN114748385A

Abstract

The invention discloses a composition and eye cream with effects of resisting wrinkles, removing dark circles and improving pouches, and belongs to the field of cosmetics. The eye cream contains the following compositions: arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate, soluble collagen and plant essence extract; the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, peony root extract, ginkgo biloba extract and achyranthes bidentata extract. According to the invention, natural effective components in the plant essence extract are reasonably selected and matched with arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate and soluble collagen, and the dosage of each component is reasonably controlled, so that each component can fully play respective role and cooperate with each other, and the obtained whitening and moisturizing essence has the remarkable effects of improving the moisture content of stratum corneum around eyes, the skin elasticity, the melanin removal and the pouch of eyes.

Description

Composition and eye cream with effects of resisting wrinkles, removing dark eye circles and improving eye bags

Technical Field

The invention relates to the technical field of cosmetics, in particular to a composition and eye cream with effects of resisting wrinkles, removing dark eye circles and improving eye bags.

Background

With the aging of facial skin, the problems of fine lines, crow's feet, dark circles, bags under the eyes and the like are more prominent. With the age, the epidermal tissues of the skin of the eyes become dry and thin, collagen and elastin are gradually lost, and the metabolism of the eyes is slow, so that eye wrinkles, black eyes and eye bags are formed. The existence of fine lines, dark circles and bags in eyes seriously influences the personal image, and is a trouble of the masses, especially women in siblings for many years.

In order to delay skin aging around eyes, in addition to ensuring sufficient sleep and establishing good living habits and eye habits in daily life, eye cosmetics are used in a common method, and at present, eye cosmetics on the market are many, but the eye cosmetics are not obvious in using effect, and some products even cause adverse reactions such as oil particles and allergy, so that an eye cream product capable of safely and effectively solving the problem of eye skin is needed.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a composition and an eye cream with the functions of resisting wrinkles, removing dark eye circles and improving under-eye puffiness so as to solve the problems in the technical background.

In order to achieve the purpose, the invention is realized by the following technical scheme:

in one aspect, the invention provides a composition with effects of resisting wrinkles, removing dark circles and improving pouches, which comprises the following raw materials: arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate, soluble collagen and plant essence extract; the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, peony root extract, ginkgo biloba extract and achyranthes bidentata extract.

The composition with the effects of resisting wrinkles, removing dark circles and improving pouches comprises the following raw materials in parts by weight: 5-10 parts of arginine/lysine polypeptide, 3-8 parts of hydroxypropyl tetrahydropyrane triol, 1-3 parts of retinol palmitate, 1-5 parts of soluble collagen and 10-15 parts of plant essence extract; the plant essence extract comprises a saffron extract, an angelica extract, a ligusticum wallichii root extract, a salvia miltiorrhiza root extract, a peony root extract, a ginkgo leaf extract and a achyranthes bidentata extract, and the weight part ratio of the saffron extract, the angelica extract, the ligusticum wallichii root extract, the salvia miltiorrhiza root extract, the peony root extract, the ginkgo leaf extract and the achyranthes bidentata extract is 1 (0.5-2) to 0.5 (1-3) to 1.

Preferably, the composition with the effects of resisting wrinkles, removing dark circles and improving pouches comprises the following raw materials in parts by weight: 5-10 parts of arginine/lysine polypeptide, 3-8 parts of hydroxypropyl tetrahydropyrane triol, 1-3 parts of retinol palmitate, 1-5 parts of soluble collagen and 10-15 parts of plant essence fermentation extract.

Further preferably, the preparation method of the plant essence fermentation extract comprises the following steps:

(1) Pretreatment of raw materials: mixing saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, peony root extract, ginkgo leaf extract and achyranthes bidentata extract in proportion, and mixing the mixture according to the weight ratio of 1: (1-5), adding deionized water, performing ultrasonic treatment at 70-80 ℃ for 20-30 min, taking out, and cooling to 35-37 ℃ to obtain a mixed solution;

(2) Adding an aspergillus strain culture solution into the mixed solution obtained in the step (1), wherein the addition amount of the aspergillus strain culture solution is 10-20% of the mixed solution, fermenting at constant temperature of 35-37 ℃, the stirring speed is 120-150 rpm, and fermenting for 48-72 h to obtain a fermented product;

(3) And (3) autoclaving the fermentation product obtained in the step (2) at 120 ℃ for 30 minutes, and freeze-drying to form a powder type plant essence fermentation extract.

Preferably, the strain contained in the culture solution of Aspergillus strain in step (2) comprises Aspergillus niger or Aspergillus oryzae at a concentration of 10 ⁶ ～10 ⁷ Per ml; further preferably, the strain contained in the culture solution of Aspergillus strain in step (2) is Aspergillus oryzae.

On the other hand, the invention provides eye cream with the effects of resisting wrinkles, removing black eyes and improving eye bags, which comprises 10-21% of the composition with the effects of resisting wrinkles, removing black eyes and improving eye bags, 3-5% of Daphnia lancifera Gaertn fruit oil, 1-3% of squalane, 0.1-0.5% of hydrogenated lecithin, 0.1-4% of phytosterol and the balance of deionized water in parts by weight.

The crocus sativus extract or saffron crocus extract has the effects of promoting blood circulation, resisting inflammation, resisting oxidation and the like, plays the roles of whitening, sun screening and resisting aging by improving skin blood circulation, promoting skin metabolism, eliminating free radicals, inhibiting melanin deposition, accelerating freckle removal and decoloration, absorbing ultraviolet rays and the like, and has the treatment effect on contact dermatitis, seborrheic dermatitis, pruritus and neurodermatitis;

the angelica extract can be used for a plurality of cosmetics for promoting blood circulation, such as hair growth, regulating skin secretion, supplementing nutrition, dissolving black eye and the like based on the functions of promoting blood circulation, removing blood stasis and improving skin microcirculation of angelica; the proliferation of the angelica on vascular endothelial cells shows that the angelica has the function of solidifying capillary vessels while promoting blood circulation; the angelica has good SOD-like activity, inhibits membrane lipid peroxidation and free radical reaction caused by superoxide radical, protects the damage of membrane lipid and other mechanisms to tissues by combining with biological membrane phospholipid to antagonize free radicals, and has special effects on dark complexion or sore swelling caused by free radical retardation;

the ligusticum wallichii root extract can inhibit the generation of free radicals and effectively eliminate oxygen free radicals; at lower concentration, the activity of melanocyte can be effectively inhibited, and the generation of melanin is reduced, but the cytotoxicity effect is shown when the concentration is too high; it also has moisture absorption and moisture keeping effects.

The salvia miltiorrhiza root extract can moisten and nourish the skin, remove spots, enhance the luster and elasticity of the skin, prevent the formation of wrinkles and further play a role in resisting aging.

Radix Paeoniae extract has effects of activating skin and resisting aging, and can be used in anti-aging cosmetic, and can inhibit elastase, eliminate free radicals, inhibit nitrogen oxide generation, and promote cathepsin activity. And has better moisture retention capability.

The main bioactive components of the ginkgo leaf extract comprise flavonoids, terpene lactones, organic acids, organic alcohols, minerals, vitamins, amino acids and the like. Wherein the flavone is a powerful scavenger for oxygen free radicals, and can protect skin cells from excessive oxidation of oxygen free radicals, protect dermal cells, improve blood circulation, and prevent oxidation of cells, thereby prolonging the life of skin cells. Meanwhile, the formation speed of the lipid peroxide is reduced, and the anti-aging capability of the lipid peroxide is enhanced. Lactones in folium Ginkgo extract can accelerate metabolism and enhance cell activity.

The arginine/lysine polypeptide has good anti-aging and anti-wrinkle effects. Hydroxypropyl tetrahydropyrane triol, commonly known as vitreous factor, is a xylose derivative with an anti-aging active substance, and can promote the synthesis of collagen, make skin more tough and elastic, improve fine lines and prevent aging. The retinol palmitate has transdermal absorption, keratinization resistance, collagen and elastin growth stimulation, and epidermal and dermal thickness increase; enhancing skin elasticity, eliminating wrinkle, promoting skin regeneration, and maintaining skin activity.

One of the ideas of the invention is that plant essence extract is added into the functional components (composition) of the eye cream, and 7 components of saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, peony root extract, ginkgo biloba extract and achyranthes bidentata extract are selected, arginine/lysine polypeptide, hydroxypropyl tetrahydropyran triol, retinol palmitate, soluble collagen and the plant essence extract are compounded, and the proportion of the 7 selected components of the plant essence extract is set, so that the plant essence extract, the arginine/lysine polypeptide, hydroxypropyl tetrahydropyran triol, retinol palmitate and soluble collagen have better effects of moisturizing, removing wrinkles, removing melanin and improving eye bags under the synergistic action.

The invention also discloses a method for preparing the plant essence fermentation extracting solution, which is characterized in that aspergillus strain culture solution is added in the preparation process of the plant essence fermentation extracting solution for fermentation to further enhance the effects of moisturizing and removing dark circles, and the fermentation activity plant essence fermentation extracting solution obtained by fermentation of the aspergillus strain culture solution provides whitening and freckle removing activity and anti-aging activity.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, natural effective components in the plant essence extract are reasonably selected and matched with arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate and soluble collagen, and the dosage of each component is reasonably controlled, so that each component can fully play respective role and cooperate with each other, and the obtained whitening and moisturizing essence has the remarkable effects of improving the moisture content of stratum corneum around eyes, the skin elasticity, the melanin removal and the pouch of eyes. The whitening and moisturizing essence disclosed by the invention does not contain components harmful to human bodies, has no irritation to skin, does not cause anaphylactic reaction, and is safe and effective eye cream for resisting wrinkles, removing dark eye circles and improving eye bags.

Drawings

FIG. 1 is a graph showing the change of the periocular skin elasticity R2 value at 2,4 and 8 weeks in example 3; in fig. 1, "×" indicates P <0.05 compared to the blank of formulation 3; ". Indicates that P <0.05 compared to the formulation 6 test group; "#" indicates that P <0.05 compared to the formulation 3 test group.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It is to be noted that the features in the following embodiments and examples may be combined with each other without conflict.

The application introduces part of the raw materials:

examples arginine/lysine polypeptide, alias L-arginine/L-lysine polypeptide, CAS:31014-78-5, available from daydream medical science and technology, inc., shanghai;

examples hydroxypropyl tetrahydropyran triol, commonly known as bose, CAS:439685-79-7, 99% pure, available from Shandong Mingfeng pharmaceutical science and technology Limited;

examples retinol palmitate, CAS:79-81-2, purity 99%, purchased from Hebei Zhentian food additive Co Ltd;

in the embodiment, the soluble collagen can be selected from fish collagen, the purity of which is 99 percent, and the soluble collagen is purchased from Ningbo Dingyuan food science and technology limited company;

in the examples, the extract of saffron, also called saffron extract, CAS:84604-17-1, available from Avotadaoule International trade of Emalto (Shanghai) Inc.;

examples angelica extract, CAS:4431-01-0, available from Sakyo Seiki science and technology Limited;

in examples, chuanxiong rhizome extract, CAS number: 76494-51-4 available from western Asian reagent (western chemical technology, shandong, co., ltd.);

examples salvia root extract, CAS:23028-17-3, available from Shanxi Send biochemicals Co., ltd;

in the examples, the extract of peony root, CAS:84929-40-8, available from seian shancheng good biotechnology limited;

examples ginkgo biloba extract, CAS:90045-36-6, available from Wolfra chemical technology (Wuhan) Inc.;

in the examples, the content of achyranthes bidentata extract: 20, from downy stonite biotechnology, ltd;

in the embodiment, the aspergillus niger is purchased from China general microbiological preservation management center, and the strain number is as follows: CGMCC 3.1454;

in the examples, aspergillus oryzae is purchased from China general microbiological culture Collection center, and the strain number is: CGMCC 3.334.

Example 1

The embodiment provides a composition with the effects of resisting wrinkles, removing dark circles and improving pouches, which is used as an active ingredient of an eye cream and comprises the following components (different formulas of plant essence extracts in the components) in parts by weight:

a component a:8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract to the achyranthes bidentata extract is 1.

And (b) component b:8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract to the achyranthes bidentata extract is 1.

And (c) component: 8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract to the achyranthes bidentata extract is 1.

Comparative example 1

The composition comprises the following components (the formula of the plant essence extract in each component is different) in parts by weight:

a component a:8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, ligusticum chuanxiong hort root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract (the proportion is 0, which is equivalent to the removal of the angelica extract, the same below), the ligusticum wallichii root extract, the salvia miltiorrhiza root extract, the paeonia lactiflora root extract, the ginkgo leaf extract and the achyranthes bidentata extract is 1.

And (b) component c:8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract (the proportion is 0, which is equivalent to removal) to the achyranthes bidentata extract is 1.

A component d:8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract to the achyranthes bidentata extract is 1.

And (e) component: 8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence extract; wherein the plant essence extract comprises saffron extract, ligusticum chuanxiong hort root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract and achyranthes bidentata extract; the weight ratio of the saffron extract to the angelica extract to the ligusticum wallichii root extract to the salvia miltiorrhiza root extract to the peony root extract to the ginkgo biloba extract to the achyranthes bidentata extract is 1.

Example 2

The embodiment provides a composition with effects of resisting wrinkles, removing dark circles and improving pouches, which comprises the following raw materials in parts by weight: 8 parts of arginine/lysine polypeptide, 5 parts of hydroxypropyl tetrahydropyrane triol, 2 parts of retinol palmitate, 3 parts of soluble collagen and 12 parts of plant essence fermentation extract. The difference lies in that the plant essence extract of the example 1 is replaced by the plant essence fermented extract, and the plant essence fermented extract adopts the plant essence extract of the component b in the example 1 as the fermentation raw material;

in this embodiment, the preparation method of the plant essence fermentation extract comprises the following steps:

(1) Pretreatment of raw materials: mixing the saffron extract, the angelica extract, the ligusticum wallichii root extract, the salvia miltiorrhiza root extract, the paeonia lactiflora root extract, the ginkgo leaf extract and the achyranthes bidentata extract according to the proportion of the formula b in example 1 (1: (1-5), adding deionized water, performing ultrasonic treatment at 70-80 ℃ for 20-30 min, taking out, and cooling to 35-37 ℃ to obtain a mixed solution;

(3) And (3) carrying out autoclaving on the fermentation product obtained in the step (2) at 120 ℃ for 30 minutes, and freeze-drying to form a powder-type plant essence fermentation extract.

In this example, the culture solution of Aspergillus strain in step (2) contained Aspergillus oryzae in a concentration of 10 ⁶ ～10 ⁷ /ml。

Example 3

This example 1 provides an eye cream with anti-wrinkle, black eye-circle-removing and pouch-improving effects, which comprises, by weight, 10 to 21% of the composition with anti-wrinkle, black eye-circle-removing and pouch-improving effects described in any one of the above examples 1, 1 to e of comparative examples 1, and 3, 3 to 5% of sorrel oil, 1 to 3% of squalane, 0.1 to 0.5% of hydrogenated lecithin, and 0.1 to 4% of phytosterols, and the balance of deionized water; the eye cream is prepared by conventional eye cream preparation method. Further, the paint comprises the following components in percentage by mass as shown in the following table 1:

TABLE 1

Test example 1 eye cream safety test

55 healthy volunteers with wrinkles, dark circles or under-eye puffiness on the skin around the eyes and no skin allergy history are selected, the women with the ages of 30-60 years and the average age of 38 +/-2 years are randomly divided into 11 groups, 5 persons in each group with the average age of 38 +/-2 years are respectively smeared with the eye creams prepared by the formulas 1-11 in the example 3. When in use, the eye cream is dipped with fingers to obtain the size of the eye cream soybean, is uniformly coated around eyes, and is coated according to a conventional eye cream coating mode to be completely absorbed by skin. The results show that: after the eye cream prepared by the formula 1-11 in the embodiment 3 is smeared for 2-8 h, allergic reactions such as redness of the skin of eyes and the like are not seen, which indicates that the eye cream of the invention is safe and has no adverse phenomenon.

Test example 2 evaluation of anti-wrinkle (increase of skin moisture content and elasticity) and dark circle removal effects of eye cream

1. The test method comprises the following steps: 176 healthy volunteers are selected, women with wrinkles and black eyes on the skin around eyes are aged between 30 and 60 years, the average age is 38 +/-2 years, the women are randomly divided into 11 groups, 16 people in each group are coated with the eye cream prepared by the formula 1-11 in the embodiment 3, 11 groups are coated with the eye cream prepared by the formula 1-11 in the embodiment 3, the skin around the left eye or the right eye is randomly selected as a test group, the skin around the other eye is used as a blank group, the volunteers are coated with the corresponding eye cream (used in the test group) once in the morning and evening every day, and the blank group is coated with a placebo eye cream.

2. And (3) testing: visits were 1 at 0,2,4 and 8 weeks for 4 total times. Before each test, a test subject needs to clean eyes with clear water and dry the eyes with a paper towel without fluorescent agent, sits still for at least 20min under a constant temperature and humidity environment (the temperature is 20-22 ℃, and the relative humidity is 40-60%), and respectively measures the positions of the fixed areas around the left and right eyes of the test subject by using a cuticle moisture content tester (MSC 1001 cuticle moisture content tester, delfin corporation, finland), a skin elasticity tester (Cutomer @ dual MPA580 skin elasticity tester, germany CK corporation) and a skin color probe (skin color tester, germany Courage + Khazaka); recording differences of the stratum corneum water content value, the elasticity R2 value and the skin ITA value, and measuring each measuring area for 3 times to obtain an average value;

4. the stratum corneum moisture content values are shown in tables 2 and 3;

TABLE 2 skin stratum corneum moisture content statistical table around eyes (test group)

Wherein, the change rate of the moisture content of the skin stratum corneum of the test group in table 2 = [ (moisture content of the skin stratum corneum of n back visits of the test group-initial moisture content of the skin stratum corneum of the test group)/initial moisture content of the skin stratum corneum of the test group ] × 100%

TABLE 3 moisture content influence of the skin stratum corneum around the eyes (blank group)

Wherein, the change rate of the moisture content of the skin stratum corneum of the blank group in table 3 = [ (the moisture content of the skin stratum corneum of n times of back visits of the blank group-the moisture content of the initial skin stratum corneum of the blank group)/the moisture content of the initial skin stratum corneum of the blank group ] × 100%

As can be seen from tables 2 and 3, the moisture content of the stratum corneum of the skin around the eyes at week 0 in the test group is the lowest, after the eyes 2,4 and 8 weeks use the compound polypeptide eye cream, the moisture content of the stratum corneum shows a gradual rising trend, and compared with the moisture content of the stratum corneum before the test (week 0) in week 8 in the test group, the change rate of the formulas 1 to 5 reaches 30.95 to 32.45 percent, and compared with the corresponding blank group, the significant difference exists; the change rate of the formula 6-10 is 14.89-15.69%, and compared with the corresponding blank group, the change rate has no significant difference; the rates of change for formulations 1-5 were significantly different compared to formulation 6. The change rate of formula 11 reached 43.27%, which was significantly different from formulas 1-5. The change rate of the moisture content of the skin cuticle around the eyes is continuously increased after the eye cream is used, so that the eye cream has the effect of better improving the moisture content of the skin cuticle around the eyes.

5. The values for the elasticity R2 are shown in table 4, table 5 and figure 1;

TABLE 4 statistical table of the elasticity of skin around the eyes R2 value (test group)

Wherein, the change rate of the skin elasticity R2 value of the test group in table 4 = [ (n-times return visit skin elasticity R2 value of test group-initial skin elasticity R2 value of test group)/initial skin elasticity R2 value of test group ] × 100%

TABLE 5 periocular skin elasticity R2 value statistical table (blank group)

Wherein, the change rate of the skin elasticity R2 value of the blank group = [ (n-times of return visits of the blank group skin elasticity R2 value-blank group initial skin elasticity R2 value)/blank group initial skin elasticity R2 value ] × 100% in table 5

The elasticity R2 value represents the ratio of the amount of springback of the skin in the absence of negative pressure to the maximum amount of stretching in the presence of negative pressure, and the closer the ratio is to 1, the better the skin elasticity is. As can be seen from table 4, table 5 and fig. 1, the elasticity R2 value at 0 week in the test group is the lowest, and the elasticity score shows a gradual increase trend after 2,4 and 8 weeks of the eye cream with the polypeptide complex, as shown in fig. 1, the change rate of the formulations 1 to 5 reaches 19.85 to 23.43% compared with the elasticity R2 value at 8 week in the test group before the test (0 week), and has a significant difference (P < 0.05) compared with the corresponding blank group; the change rate of the formula 6-10 is 3.58-4.12%, and compared with the corresponding blank group, the change rate has no significant difference; compared with the formula 6, the change rates of the formulas 1 to 5 have significant differences, which indicates that the functional components (composition) of the eye cream are added with plant essence extracts, 7 components of saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba extract and achyranthes bidentata extract are selected, arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate and soluble collagen are compounded with the plant essence extracts, and the proportion of the 7 selected components of the plant essence extracts is set so that the plant essence extracts, the arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate and the soluble collagen have better moisturizing and wrinkle removing effects under the synergistic action. The change rate of the formula 11 reaches 38.27%, and compared with the formula 1-5, the formula has significant difference (P is less than 0.05), which indicates that the moisture-preserving and black eye-removing effects of the plant essence fermentation extract can be further enhanced by adding the aspergillus strain culture solution for fermentation in the preparation process of the plant essence fermentation extract, and the reason is that the fermentation activity plant fermentation extract obtained by fermentation of the aspergillus strain culture solution provides anti-aging activity.

6. The evaluation results of removing dark circles according to the difference of skin ITA values are shown in table 6;

wherein, the judgment standard of black eye removing is as follows: the difference value of the ITA value of the skin color of each volunteer test group is taken, the value of the position 15mm and the position 25mm directly below the center of the pupil is taken, and the difference value X between the two points is calculated ₀ As an initial value, the difference X between two points was calculated using the test eye cream n times (number of revisits) respectively _n The general formula is judged as follows:

and (4) invalidation: x ₀ /4>X ₀ -X _n (n = 1 return visit, 2 return visits, 4 return visits 8230; the same applies below);

improvement; x ₀ /4≤X ₀ -X _n <X ₀ /3；

The effect is shown: x ₀ /3≤X ₀ -X _n <X ₀ ；

And (3) healing: x ₀ ≤X ₀ -X _n 。

TABLE 6 evaluation table of black eye removing effect of different eye creams after 8 weeks

Group of	Number of examples	Recovery (human/%)	Significant effect (human/%)	Improvement (people/%)	No effect (human/%)
						Formulation 1	16	0(0)	5(31.25)	11(68.75)	0(0)
Formulation 2	16	1(6.25)	5(31.25)	10(62.5)	0(0)
						Formulation 3	16	1(6.25)	7(43.75)	9(56.25)	0(0)
Formulation 4	16	0(0)	5(31.25)	11(68.75)	0(0)
						Formulation 5	16	1(6.25)	6(37.5)	10(62.5)	0(0)
Formulation 6	16	0(0)	1(6.25)	13(81.25)	2(12.5)
						Formulation 7	16	0(0)	3(18.75)	12(75)	1(6.25)
Formulation 8	16	0(0)	1(6.25)	12(75)	3(18.75)
						Formulation 9	16	0(0)	2(12.5)	11(68.75)	2(12.5)
Formulation 10	16	0(0)	1(6.25)	11(68.75)	4(25)
						Formulation 11	16	2(12.5)	10(62.5)	4(25)	0(0)

From table 6, it can be seen that when the eye cream prepared by the formulas 1-5 is used by volunteers, the level of melanin in the eyes is obviously reduced, the significant efficiency (healing and significant effect) reaches 31.25-50%, the rate of improvement reaches 50-68.75%, and the inefficiency reaches 0%; the eye cream prepared by the formula 11 has the advantages that the level of melanin in eyes is obviously reduced, the obvious effect rate (healing and obvious effect) reaches 75 percent, the improvement rate reaches 25 percent, and the ineffective rate is 0 percent; the effect is obviously better than that of the eye cream prepared by the formula 6-10, and the eye cream prepared by the formula 11 has the best use effect, which shows that the repair eye cream provided by the invention has better depigmentation effect. The significant efficiency of the formulas 1 to 5 is remarkably improved compared with the significant efficiency (6.25 to 18.75%) of the formulas 6 to 10, which indicates that the functional components (composition) of the eye cream are added with plant essence extracts, 7 components of saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo biloba leaf extract and achyranthes bidentata extract are selected, arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate, soluble collagen and the plant essence extracts are compounded, and the proportion of the 7 selected components of the plant essence extracts is set so that the components of the plant essence extracts, arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate and soluble collagen have better melanin removing effect under the synergistic action. The significant efficiency of the formula 11 is remarkably improved compared with that of the formulas 1 to 5, which indicates that the moisture-preserving and dark eye circle-removing effects of the plant essence can be further enhanced by adding the aspergillus strain culture solution for fermentation in the preparation process of the plant essence fermentation extract, because the fermentation activity plant essence fermentation extract obtained by the fermentation of the aspergillus strain culture solution provides the whitening and freckle-removing activity.

Test example 3 evaluation of pouch removing effect of eye cream

The test method comprises the following steps: 176 healthy volunteers are selected, women with the skin around eyes having the problem of bags under the eyes are aged between 30 and 60 years, the average age is 38 +/-2 years, the women are randomly divided into 11 groups, 16 people in each group are respectively smeared on the eye cream prepared by the formula 1-11 in the embodiment 3 by 11 groups, the skin around the left eye or the right eye is randomly selected as a test group, and the volunteers are respectively smeared on the corresponding eye cream in the morning and the evening. The pouch volume was measured at 0 weeks of use and at 8 weeks of use, and the pouch removal results were evaluated according to the percentage reduction in pouch volume, and the evaluation results are shown in table 7;

wherein the percentage reduction in pouch volume = [ (0-8-week pouch volume)/0-week pouch volume ] × 100%

Evaluation criteria for removing eye bags:

the effect is shown: the volume of the eye bags is reduced by more than 35 percent;

the method has the following advantages: the volume of the eye bags is reduced by 30-35%;

improvement: the volume of the eye bags is reduced by 0-30%;

and (4) invalidation: there was no change or increase in pouch volume.

TABLE 7 evaluation table of black eye removing effect of different eye creams after 8 weeks

Group of	Number of examples	Significant effect (human/%)	Effective (human/%)	Improvement (people/%)	No effect (human/%)
						Formulation 1	16	3(18.75)	9(56.25)	4(25)	0(0)
Formulation 2	16	4(25)	7(43.75)	5(31.25)	0(0)
						Formulation 3	16	4(25)	9(56.25)	3(18.75)	0(0)
Formulation 4	16	3(18.75)	7(43.75)	6(37.5)	0(0)
						Formulation 5	16	3(18.75)	8(50)	5(31.25)	0(0)
Formulation 6	16	0(0)	5(31.25)	9(56.25)	2(12.5)
						Formulation 7	16	1(6.25)	6(37.5)	6(37.5)	3(18.75)
Formulation 8	16	1(6.25)	7(43.75)	5(31.25)	3(18.75)
						Formulation 9	16	1(6.25)	8(50)	5(31.25)	2(12.5)
Formulation 10	16	0(0)	6(37.5)	6(37.5)	4(25)
						Formulation 11	16	9(56.25)	5(31.25)	2(12.5)	0(0)

As can be seen from Table 8, when the eye creams prepared according to the formulas 1 to 5 are respectively used by volunteers, the pouch volume is obviously reduced, the obvious effective rate reaches 18.75 to 25 percent, the effective rate reaches 43.75 to 56.25 percent, and the ineffective rate is 0 percent; the eye cream prepared by the formula 11 has the advantages that the pouch volume is obviously reduced, the obvious effective rate reaches 56.25%, the effective rate reaches 31.25%, and the ineffective rate is 0%; the effect is obviously better than that of the eye cream prepared by the formula 6-10, and the eye cream prepared by the formula 11 has the best use effect, which shows that the repair eye cream provided by the invention has better pouch removing effect. Compared with the effective rate and the effective rate of formulas 6-10, the effective rate and the effective rate of formulas 1-5 are remarkably improved, which indicates that the eye cream is prepared by compounding arginine/lysine polypeptide, hydroxypropyl tetrahydropyrane triol, retinol palmitate, soluble collagen and plant essence extracts (comprising saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, paeonia lactiflora root extract, ginkgo leaf extract and achyranthes bidentata extract), so that the plant essence extracts, the arginine/lysine polypeptide, the hydroxypropyl tetrahydropyrane triol, the retinol palmitate and the soluble collagen have better anti-wrinkle and anti-aging effects under the synergistic action, and the volume of the eye bags is reduced. Compared with the formulas 1-5, the significant efficiency of the formula 11 is remarkably improved, which indicates that the moisture-preserving, anti-wrinkle and anti-aging effects of the plant essence extract can be further enhanced by adding the aspergillus strain culture solution for fermentation in the preparation process of the plant essence fermentation extract.

The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims

1. The composition with the effects of resisting wrinkles, removing dark circles and improving pouches is characterized by comprising the following raw materials: the weight portion is as follows: 5 to 10 parts of arginine/lysine polypeptide, 3 to 8 parts of hydroxypropyl tetrahydropyran triol, 1 to 3 parts of retinol palmitate, 1 to 5 parts of soluble collagen and 10 to 15 parts of plant essence extract;

the plant essence extract comprises a saffron extract, an angelica extract, a ligusticum wallichii root extract, a salvia miltiorrhiza root extract, a peony root extract, a ginkgo leaf extract and an achyranthes bidentata extract, and the weight part ratio of the saffron extract, the angelica extract, the ligusticum wallichii root extract, the salvia miltiorrhiza root extract, the peony root extract, the ginkgo leaf extract and the achyranthes bidentata extract is 1 (0.5 to 2): 0.5;

the preparation method of the plant essence extract comprises the following steps:

(1) Pretreatment of raw materials: mixing saffron extract, angelica extract, ligusticum wallichii root extract, salvia miltiorrhiza root extract, peony root extract, ginkgo leaf extract and achyranthes bidentata extract in proportion, and mixing the mixture according to the weight ratio of 1: adding deionized water according to the proportion of (1) - (5), carrying out ultrasonic processing at 70-80 ℃ for 20-30min, taking out, and cooling to 35-37 ℃ to obtain a mixed solution;

(2) Adding an aspergillus strain culture solution into the mixed solution obtained in the step (1), wherein the addition amount of the aspergillus strain culture solution is 10-20% of the mixed solution, performing constant-temperature fermentation at the temperature of 35-37 ℃, and obtaining a fermented product after fermentation at the stirring speed of 120-150rpm for 48-72h;

(3) Sterilizing the fermentation product obtained in the step (2) at 120 ℃ for 30 minutes under high pressure, and freeze-drying to obtain a powder type plant essence fermentation extract;

the strain contained in the aspergillus strain culture solution in the step (2) comprises aspergillus niger or aspergillus oryzae.

2. The composition with anti-wrinkle, black eye circle removing and pouch improving effects as claimed in claim 1, wherein: the soluble collagen is fish collagen.

3. An eye cream with the effects of resisting wrinkles, removing black eyes and improving pouches, which is characterized by comprising, by weight, 10 to 21% of the composition with the effects of resisting wrinkles, removing black eyes and improving pouches according to claim 1, 3 to 5% of palmerosa lancifera oil, 1 to 3% of squalane, 0.1 to 0.5% of hydrogenated lecithin, 0.1 to 4% of phytosterols and the balance of deionized water.