CN108969444B - Black eye removing, anti-wrinkle firming and repairing eye cream and preparation method thereof - Google Patents

Abstract

The invention discloses a black eye removing, anti-wrinkle firming and repairing eye cream and a preparation method thereof. The repair eye cream comprises the following components: glycerol, palmitic acid, squalane, cetostearyl alcohol, methyl gluceth-10, lecithin, caprylic/capric triglyceride, an essential oil-containing nanoliposome, a coffee chervil seed extract, a red globe ginger extract, a syzygium jambos leaf extract, undecylenoyl phenylalanine, acetyl chitosamine, aescin, plant fruit seed micropowder, dextran sulfate sodium, polyacryloyl ammonium dimethyltaurate, phenoxyethanol, ethylhexyl glycerol, and the balance of deionized water. The eye cream has obvious synergistic effect among the components, has obvious effects of fading dark circles, smoothing fine lines and eliminating pouches, is fresh and easy to absorb compared with the existing eye cream, does not cause burden on eye skin, and does not form eye fat granules.

Description

Black eye removing, anti-wrinkle firming and repairing eye cream and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a black eye-circle-removing, anti-wrinkle and skin-firming repairing eye cream and a preparation method thereof.

Background

The eyes are the earliest exposed age on the face. The eye skin is the thinnest skin of the human body and is the part which moves most frequently, so the eye skin is more easily injured by the outside. The eye subcutaneous loose connective tissue is rich, soft and elastic, and the fiber structure of the loose connective tissue is distributed with abundant capillaries and nerve endings, and the walls of the capillaries are very thin and have certain permeability. Loose connective tissue is easily engorged with blood, water and forms hematomas or edema. There is little sebaceous or sweat gland distribution under the skin of the eye, and thin skin around the eye is very prone to dryness and lack of moisture. Because the skin of the eyes lacks sebaceous glands and sweat glands, the skin around the eyes does not have natural moistening capability, and much less grease can be supplied, so that the skin of the eyes is very easy to age. The skin around the eyes is sensitive and fragile, and the capillaries are extremely fine, which easily generate wrinkles, dark circles and bags.

The black eye circles, the fine lines of the eyes and the eye bags seriously affect the quality and appearance of a person, and are easy to make the person look haggard. The three components form a chain reaction, because of the increase of age, the epidermal tissue of the eye skin is dry and thin, collagen and elastin gradually run off, and the mesh structure of the fine sieve formed by interweaving collagen fibers and elastic fibers is damaged, and wrinkles with different thicknesses can appear; the metabolism of eyes is slow, the blood circulation is not smooth, and the capillary vessel is blocked. Inflammation and pigmentation are triggered to form a black eye; the eye skin is further aged, and when the eye skin is aged to a certain degree, the deposited fat can not be held, so that the eye skin is thickened and protruded, and pouches appear.

Eye cream, one of skin care cosmetics, is used to protect the relatively thin skin around the eyes, has the efficacy of moistening, and has certain effects on pouches, black eyes, crow's feet, dry lines, fine lines and the like. Eye cream products in the market at present are many, but the problems of poor permeability, difficult absorption, unobvious use effect and the like generally exist, and some products even can cause some adverse reactions such as oil particles, allergy and the like, so that a beauty and skin care product capable of safely and effectively solving the problem of eye skin is needed to meet the market demand.

Disclosure of Invention

The invention aims to provide a black eye removing, anti-wrinkle firming and repairing eye cream and a preparation method thereof. The repair eye cream contains various natural nourishing components, can effectively permeate into eye skin tissues, eliminate melanin, lighten dark eye circles, activate dermis collagen and fiber cells, and promote the reconstruction of collagen elastic fibers, thereby realizing deep skin tightening, lifting and resisting wrinkles and continuously recovering youth elasticity.

The invention is realized by the following technical scheme:

the black eye removing, anti-wrinkle firming and repairing eye cream comprises the following components in percentage by mass: 2-8% of glycerol, 1-3% of palmitic acid, 1-3% of squalane, 1-4% of cetostearyl alcohol, 101-3% of methyl gluceth-101, 0.5-4% of lecithin, 1-5% of caprylic/capric triglyceride, 6-12% of essential oil-containing nano-liposomes, 0.5-4% of coffee cherokee seed extract, 0.5-4% of red globe ginger extract, 0.5-4% of rose apple leaf extract, 1-4% of undecylenoyl phenylalanine, 1-4% of acetyl chitosamine, 1-4% of aescin, 3-7% of plant fruit seed ultrafine powder, 1-6% of dextran sulfate sodium, 0.2-1% of polyacryl dimethyl ammonium taurate, 0.01-0.2% of phenoxyethanol, 0.01-0.2% of ethylhexylglycerol and the balance of deionized water.

Preferably, the black eye removing, anti-wrinkle and skin firming repair eye cream comprises the following components in percentage by mass: 4-6% of glycerol, 1-2% of palmitic acid, 1-2% of squalane, 1-3% of cetostearyl alcohol, 101-3% of methyl gluceth, 1-3% of lecithin, 2-4% of caprylic/capric triglyceride, 8-12% of essential oil-containing nano liposome, 1-3% of small fruit coffee seed extract, 1-3% of red ball ginger extract, 1-3% of cattail leaf extract, 2-3% of undecylenoyl phenylalanine, 1-3% of acetyl chitosamine, 1-3% of aescin, 4-6% of plant fruit seed ultrafine powder, 2-5% of glucan sodium sulfate, 0.5-1% of polyacryl dimethyl ammonium taurate, 0.05-0.15% of phenoxyethanol, 0.05-0.15% of ethylhexylglycerol and the balance of deionized water.

More preferably, the black eye removing, anti-wrinkle and skin firming repair eye cream comprises the following components in percentage by mass: 5% of glycerol, 1.5% of palmitic acid, 2% of squalane, 3% of cetostearyl alcohol, 102% of methyl gluceth-102%, 3% of lecithin, 4% of caprylic/capric triglyceride, 10% of essential oil-containing nanoliposome, 3% of small fruit coffee seed extract, 2% of red globe ginger extract, 2% of syzygium jambos leaf extract, 3% of undecylenoyl phenylalanine, 1.5% of acetyl chitosamine, 2% of aescin, 5% of plant fruit seed micropowder, 4% of sodium dextran sulfate, 0.5% of polyacryloyl ammonium dimethyltaurate, 0.05% of phenoxyethanol, 0.15% of ethylhexyl glycerol and the balance of deionized water.

The plant fruit seed ultrafine powder is composed of evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed in a mass ratio of 1 (1-2) to 0.5-1 to 1-3. Preferably, the plant fruit seed ultrafine powder is composed of evening primrose seeds, amur grape seeds, purple alfalfa seeds and buckwheat seeds in a mass ratio of 1:1:0.5: 2.

The preparation of the plant fruit seed ultrafine powder comprises the following steps: mixing evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed according to the formula ratio, and crushing to obtain seed mixed powder; and adding the seed and fruit mixed powder into an airflow mill pulverizer, performing airflow milling, allowing the fully milled micro powder to pass through gaps of a sorting wheel along with airflow, and allowing the insufficiently milled micro powder not to pass through, continuing the airflow milling until the particle size of the powder is 0.2-1 mu m, and collecting the plant fruit seed ultra-fine powder.

The essential oil-containing nano liposome comprises 20-30% of solid matters by mass, and the solid matters comprise the following components in percentage by mass: 5-10% of permanent flower essential oil, 5-10% of rock rose essential oil, 8-16% of vitamin E, 35-45% of hydrogenated soybean lecithin, 5-15% of cholesterol, 5-15% of beta-sitosterol and 8-804% of tween-D.

Preferably, the mass percentage of the solid in the essential oil-containing nano liposome is 20-30%, and the formula of the solid comprises the following components in percentage by mass: 8% of permanent flower essential oil, 8% of rock rose essential oil, 14% of vitamin E, 40% of hydrogenated soybean lecithin, 12% of cholesterol, 12% of beta-sitosterol and 806% of tween-L.

The preparation method of the essential oil-containing nano liposome comprises the following steps:

(1) weighing permanent flower essential oil, rock rose essential oil, vitamin E, hydrogenated soybean lecithin, cholesterol and beta-sitosterol according to a formula, adding the permanent flower essential oil, the rock rose essential oil, the vitamin E, the hydrogenated soybean lecithin, the cholesterol and the beta-sitosterol into absolute ethyl alcohol at the temperature of 40-60 ℃, and fully dissolving to obtain an oil phase;

(2) adding tween-80 with the formula amount into PBS buffer solution at the temperature of 40-50 ℃, slowly adding while stirring, and fully mixing to prepare a solution with the tween-80 of which the mass percent is 0.8-2.4%, so as to obtain a water phase;

(3) injecting the water phase into the oil phase by using an injector under the continuous stirring of a magnetic stirrer, fully mixing for 0.5-1 h, then carrying out rotary evaporation at the temperature of 28-32 ℃ and under the pressure of 0.1-1 MPa to remove ethanol, carrying out oscillation hydration reaction in a water bath at the temperature of 30-35 ℃ for 1-2 h to obtain liposome suspension, and adjusting the particle size of the liposome suspension to 100-150 nm by using an injector type filter to obtain the essential oil-containing nano liposome.

In addition, the invention also provides a preparation method of the eye cream for removing dark eye circles, resisting wrinkles and tightening skin and repairing, which comprises the following steps:

(1) adding deionized water, glycerol, acetyl chitosamine, plant fruit seed ultrafine powder, dextran sulfate sodium and polyacryl dimethyl ammonium taurate into a water phase pot according to the formula amount, heating to 70-75 ℃, and uniformly stirring to obtain a water phase;

(2) adding palmitic acid, squalane, cetostearyl alcohol, methyl gluceth-10, lecithin and caprylic/capric triglyceride into an oil phase pot according to the formula ratio, heating to 70-75 ℃, and uniformly stirring to obtain an oil phase;

(3) pumping the water phase into a vacuum emulsifying machine, starting stirring and vacuum, sucking the oil phase into the emulsifying machine under the conditions of 80-85 ℃, vacuum degree of-0.06-0.04 MPa and stirring speed of 60-80 r/min to obtain a mixed solution, and homogenizing for 10-15 min at 2000-3000 r/min;

(4) after homogenizing, keeping the vacuum degree and stirring speed unchanged, cooling to 40-45 ℃, adding the essential oil-containing nano liposome, the small fruit coffee seed extract, the red globe ginger extract, the syzygium jambos leaf extract, the undecylenoyl phenylalanine, the aescin, the phenoxyethanol and the ethylhexyl glycerin, continuously stirring uniformly, cooling to room temperature, discharging, and filling to obtain the eye cream for removing black eye circles, resisting wrinkles and tightening the skin.

The permanent flower essential oil in the formula is rich in neryl acetate, neryl butyrate, nerol and other substances, can promote the regeneration of skin cells, activate skin cells, promote the regeneration of keratin interstitium and collagen, accelerate the speed of wound repair, and has obvious effects on smoothening fine wrinkles and tightening eye skin; the rock rose essential oil is rich in substances such as alpha-terpineol, borneol acetate, labdanol and the like, can dredge blood vessels, promote blood circulation, promote lymph toxin expelling and tissue detumescence, and has obvious effects of fading dark eye circles of eyes and eliminating pouches. The invention compounds the permanent flower essential oil and the rock rose essential oil to prepare the nanoliposome which is beneficial to the absorption of eye skin, can be effectively absorbed by the skin through the cuticle, greatly improves the stability of the essential oil and reduces the irritation of the essential oil. Meanwhile, a small amount of vitamin E is added into the formula, so that skin cells can better absorb essential oil nutrients, and the essential oil-containing nano liposome can better play the effects of lightening dark eye circles of eyes, smoothening fine lines and eliminating pouches.

In addition, the extract of the seed of the COFFEA ARABICA (COFFEA ARABICA) contained in the formula has an inhibiting effect on trypsin, can inhibit the trypsin from degrading elastin, has a protecting effect on cortical elastin fibers, and fundamentally solves the reason of wrinkle generation: the loss of collagen elastic fibers has obvious effects of smoothing fine lines and inhibiting the generation of wrinkles. The extract of Zingiber ZERUMBET (Zingiber ZERUMBET) has effects of improving eyesight and nourishing liver, improving eyesight, removing dampness, promoting urination, promoting blood circulation, promoting skin metabolism, activating skin microcirculation by vasodilatation, and eliminating pouch and dark eye ring. The extract of folium Syzygium JAMBOS (SYZYGIUM JAMBOS) has effects of balancing skin pH, astringing follicular orifice, repairing damaged skin, and is rich in antioxidant components, and can effectively penetrate into stratum corneum deep to prevent formation of melanine, prevent black spot and mottle, and eliminate dark eye. The three components are compounded for use, and the purposes of lifting and tightening eye skin, smoothening fine wrinkles and eliminating under-eye bags and black eyes are achieved by inhibiting the loss of collagen elastic fibers, promoting blood circulation, promoting skin metabolism, repairing damaged skin and resisting oxidation.

The undecylenoyl phenylalanine enables the melanocytes to fail to produce tyrosinase by competitively combining with melanin stimulating hormone receptor MC1-R on the melanocytes, thereby inhibiting the activity of the melanocytes, reducing the generation of melanin and enhancing the effect of the syzygium jambos leaf extract on eliminating dark circles. The acetyl chitosamine can promote hyaluronic acid synthesis, enhance skin hydration ability, improve eye skin elasticity, and enhance the fine lines smoothing effect of the coffee cherokee seed extract. Aescin can promote blood circulation, improve puffiness around eyes, and enhance the effect of red ball ginger extract in eliminating eye bags.

The plant fruit seed ultrafine powder in the formula is a mixture of evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed ultrafine powder, and can improve the smoothness effect of eye cream, improve the freshness, prevent pore blockage and prevent the formation of fat particles.

The repairing eye cream provided by the invention is a fine paste in appearance, has the pH of 6.2-7.1, has the viscosity of 10000-15000 mpa.s, is heat-resistant at 45 ℃ for 24 hours, has no obvious character change after the room temperature is recovered, and has no obvious character change after the room temperature is recovered and has cold resistance at-18 ℃ for 24 hours.

The ingredients of the formulation of the present invention are commercially available products unless otherwise specified. Among them, coffee cherokee (coffeaabaca) seed extract was purchased from Active Concepts, usa. Zingiber ZERUMBET (Zingiber ZERUMBET) extract was obtained from Saianfeng Biotech Ltd. The extract of the leaves of Syzygium JAMBOS (SYZYGIUM JAMBOS) was obtained from Sailanry Biotechnology, Inc. The CAS number for undecylenoyl phenylalanine is: 175357-18-3, available from Siam Jia Bongming Biotech, Inc. The CAS number of the acetylcysteine is: 7512-17-6, available from Beis Biotech, Inc., Guangzhou. The CAS number of escin is: 6805-41-0, available from Hubei Jusheng science and technology Co.

Evening primrose seeds were purchased from ambo pharmaceutical ltd, bo zhou; vitis amurensis seeds were purchased from the company of limited liability in the pharmaceutical industry, jia guan zhou city; alfalfa seeds and buckwheat seeds were purchased from renhuitang chinese medicinal materials sales limited, Bozhou.

Permanent flower essential oil, native France, available from Yake Yeti commercial Co., Ltd, Beijing; petrorose essential oil, native France, purchased from Shangye Rieger commerce, Inc. of Beijing; vitamin E was purchased from Noxin Biotechnology, Inc., Guangzhou.

Compared with the prior art, the invention has the following beneficial effects:

(1) the eye cream has scientific proportion, has obvious synergistic effect on nutrient components such as small fruit coffee seed extract, red globe ginger extract, Chinese rose leaf extract, undecylenoyl phenylalanine, acetyl chitosamine, aescin, nano liposome containing essential oil, plant fruit seed ultrafine powder and the like, has obvious effects on fading black eye circles, smoothing fine wrinkles and eliminating eye bags, and is fresh and easy to absorb compared with the existing eye cream, cannot cause burden on eye skin, and cannot form eye fat granules.

(2) The formula structure of the eye cream is optimized, and the purposes of lifting and tightening eye skin, smoothening fine lines and eliminating under-eye bags and black eyes are achieved by inhibiting the loss of collagen elastic fibers, promoting blood circulation and activating blood circulation, promoting skin metabolism, promoting the excretion of retained and silted fat in veins, eliminating deep melanin, repairing damaged skin, moisturizing and relieving and resisting oxidation.

(3) The repair eye cream disclosed by the invention has low skin irritation, does not cause anaphylactic reaction, is mild and safe in property, good in permeability, easy to absorb, and fresh and non-sticky to skin after being used; and the preparation process is simple, the conditions are controllable, and the process is stable.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.

Example 1 preparation of nanoliposomes containing essential oils

Example 1 the essential oil-containing nanoliposome contains 25% by mass of solids, specifically, the formulation of the solids comprises the following components by mass:

calculated according to 25g of solid content in 100g of nano liposome containing essential oil,

the preparation method of the group A comprises the following steps:

(1) weighing 2g of permanent flower essential oil, 2g of rock rose essential oil, 3.5g of vitamin E, 10g of hydrogenated soybean lecithin, 3g of cholesterol and 3g of beta-sitosterol, adding into absolute ethyl alcohol at 50 ℃, and fully dissolving to obtain an oil phase;

(2) adding 1.5g of tween-80 into PBS buffer solution at the temperature of 50 ℃, slowly adding while stirring, and fully mixing to prepare a solution with the mass percent of tween-80 being 1.5%, so as to obtain a water phase;

(3) continuously stirring by a magnetic stirrer, injecting the water phase into the oil phase by using an injector, fully mixing for 1h, performing rotary evaporation at 30 ℃ and 0.8MPa to remove ethanol, performing oscillation hydration reaction in a water bath at 32 ℃ for 2h to obtain liposome suspension, and adjusting the particle size of the liposome suspension to 100-150 nm by using an injector type filter to obtain the essential oil-containing nano liposome.

B. Preparation of group C reference is made to group A.

EXAMPLE 2 plant fruit seed micropowder preparation

Example 2 the formulation of plant fruit seed micropowder is: the plant fruit seed ultrafine powder comprises the following components by weight based on 100g of plant fruit seed ultrafine powder:

components	Group A	Group B	Group C
				Evening primrose seed	22.2g	16.7g	18.2g
Vitis amurensis seed	22.2g	16.7g	36.4g
				Alfalfa seeds	11.2g	16.7g	9.0g
Buckwheat seed	44.4g	49.9g	36.4g

The preparation method comprises the following steps: mixing evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed according to the formula ratio, and crushing to obtain seed mixed powder; and adding the seed and fruit mixed powder into an airflow mill pulverizer, performing airflow milling, allowing the fully milled micro powder to pass through gaps of a sorting wheel along with airflow, and allowing the insufficiently milled micro powder not to pass through, continuing the airflow milling until the particle size of the powder is 0.2-1 mu m, and collecting the plant fruit seed ultra-fine powder.

Example 3 preparation of a repair eye cream

Example 3 the formulation of the repair eye cream consists of: the paint comprises the following components in percentage by mass:

the preparation method comprises the following steps:

(1) adding deionized water, glycerol, acetyl chitosamine, plant fruit seed ultrafine powder, dextran sulfate sodium and polyacryl dimethyl ammonium taurate into water phase pot, heating to 75 deg.C, and stirring to obtain water phase;

(2) adding the formula amounts of palmitic acid, squalane, cetostearyl alcohol, methyl gluceth-10, lecithin and caprylic/capric triglyceride into an oil phase pot, heating to 75 ℃, and uniformly stirring to obtain an oil phase;

(3) pumping the water phase into a vacuum emulsifying machine, starting stirring and vacuum, sucking the oil phase into the emulsifying machine at 80 deg.C, vacuum degree of-0.04 MPa and stirring speed of 80r/min to obtain mixed solution, and homogenizing at 3000r/min for 12 min;

(4) after homogenizing, keeping vacuum degree and stirring speed unchanged, cooling to 45 ℃, adding the essential oil-containing nano liposome, the small fruit coffee seed extract, the red globe ginger extract, the syzygium jambos leaf extract, the undecylenoyl phenylalanine, the aescin, the phenoxyethanol and the ethylhexyl glycerin, continuously stirring uniformly, cooling to room temperature, discharging, and filling to obtain the repair eye cream.

Comparative example 1 preparation of eye cream

Comparative example 1 the formulation of the repair eye cream was composed of: the paint comprises the following components in percentage by mass:

the preparation method refers to example 3.

Test example I, safety test of eye cream repair

Number of test population: the test method comprises the following steps of 1, 110 women, 30-60 years old, healthy skin of a subject, no skin allergy history, 11 groups of 10 persons in each group, and 38 +/-2 years old as the average age, wherein the test groups are respectively 1-11 groups, wherein the test groups 1-5 use the eye cream prepared by the formula 1-5 in the example 3, and the test groups 6-11 use the eye cream prepared by the formula 6-11 in the comparative example 1.

The using method comprises the following steps: dipping the millet granules with the size of the eye cream by a ring finger, dropping a little under the eyes, slightly pulling down the lower eyelid of the eyes by the left hand, and then performing annular slight massage along the inner canthus, the upper eyelid, the tail of the eyes and the lower eyelid, generally for about 4-5 circles; and finally, gently patting the corresponding eyes by using ring fingers of two hands to ensure that the eyes are completely absorbed by the skin.

The results show that: after the eye cream prepared by the formulas 1 to 5 of the invention example 3 and the comparative examples 6 to 11 is smeared for 8 hours and 12 hours, allergic reactions such as redness of the skin of the eyes and the like are not seen, which indicates that the eye cream of the invention is safe and has no adverse phenomenon.

Test example II evaluation of Black eye removing Effect of eye cream repair

Number of test population: 110 female volunteer subjects with dark skin color, color spots and dark circles around the eyes were selected, aged 25-60 years and the average age of 35 ± 5 years, and divided into 11 groups of 10 persons each, namely, test 1-5 groups and comparative 1-6 groups, wherein the test 1-5 groups used the eye creams prepared according to the formulations 1-5 of example 3, and the comparative 1-6 groups used the eye creams prepared according to the formulations 6-11 of comparative 1.

The using method comprises the following steps: after cleaning and refreshing the face in the morning and evening, a proper amount of the tested eye cream is taken and applied to the periphery of eyes, and the eye cream is evenly applied and massaged until being absorbed. Before use, testing the melanin value, the skin color L value and the skin color b value of the eye skin after 1 month, and calculating ITA degrees, wherein the larger the skin color L value is, the whiter the skin color is; the larger the value of the skin color b, the darker and yellow the skin color is; the larger the ITA value, the whiter the skin color. The results are shown in Table 1 below.

TABLE 1 evaluation of the Effect of removing dark circles by the repair eye cream

The results show that when the eye creams prepared by the formulas 1 to 5 in the example 3 are respectively used by volunteer subjects, the level of melanin in the eyes is obviously reduced, the skin color L value and the ITA DEG value are increased, the effect is obviously better than that of the eye creams prepared by the formulas 6 to 11 in the comparative example 1, and the using effect of the eye cream prepared by the formula 1 in the example 3 is the best, which indicates that the eye cream provided by the invention has better effects of removing black eye circles and improving the brightness of the eye skin. And no cellulite was observed in all groups of volunteers during the test, except for 4 eyes of the control 6 groups of volunteers.

As can be seen from the comparative example 1, the effect of reducing the level of melanin and improving the whiteness of skin is weakened due to the lack of the essential oil-containing nano liposome in the formula, which shows that the essential oil-containing nano liposome has a certain effect of improving the symptoms of dark circles; as can be seen from comparative examples 2-4, the lack of the red globe ginger extract and the rose apple leaf extract simultaneously in the formula, or the lack of the small fruit coffee seed extract and the rose apple leaf extract simultaneously, or the lack of the small fruit coffee seed extract and the red globe ginger extract simultaneously weakens the effects of reducing the melanin level and improving the skin brightness, and shows that the compounding of the small fruit coffee seed extract, the rose apple leaf extract and the red globe ginger extract has obvious synergistic effect on improving the black eye circles; from the comparative example 5, the effect of reducing the level of melanin and improving the brightness of skin is weakened by directly adding the permanent flower essential oil and the rock rose essential oil into the formula instead of in the form of liposome, which shows that the essential oil-containing nano liposome is more beneficial to skin absorption, thereby exerting better black eye removing effect; the comparative example 6 shows that the lack of plant fruit seed ultrafine powder in the formula has little influence on the effect of improving the symptoms of the dark eye circles, the prepared eye cream still has good effects of reducing melanin level and improving skin brightness, but influences the appearance of the fatty granules on the eyes of volunteer subjects, and the plant fruit seed ultrafine powder is beneficial to improving the smoothness of the eye cream and can effectively prevent the adverse reaction of the fatty granules in the using process of the eye cream.

Test example III evaluation of pouch removal effect of eye cream

Number of test population: 220 volunteer subjects with long-term pouch problems, aged 40-60 years, were selected in 11 groups of 20 persons each, test 1-5 groups and comparative 1-6 groups, respectively, wherein the test 1-5 groups used the eye creams prepared according to the formulations 1-5 of example 3, and the comparative 1-6 groups used the eye creams prepared according to the formulations 6-11 of comparative example 1.

The using method comprises the following steps: after cleaning and refreshing the face in the morning and evening, a proper amount of the tested eye cream is taken and applied to the periphery of eyes, and the eye cream is evenly applied and massaged until being absorbed. The pouch volume was measured before use, 2 months after use, respectively. The determination of the volume of the eye bags is carried out using a technique known as Fringe project, and 3D images of the region of interest are acquired by a FaceScanner and processed using Optocat software (Breuckman, EoTech, Germany).

The therapeutic effect judgment standard is as follows:

the effect is shown: the volume of the eye bags is reduced by more than 35 percent;

the method has the following advantages: the volume of the eye bags is reduced by 30-35%;

improvement: the volume of the eye bags is reduced by 0-30%;

and (4) invalidation: there was no change or increase in pouch volume.

The results are shown in Table 2 below.

TABLE 2 evaluation of pouch removal effect of repair eye cream

The results show that when the eye cream prepared by the formulas 1 to 5 in example 3 is respectively used by volunteer subjects, the pouch volume is obviously reduced, the obvious effect rate reaches more than 20 percent, and the effective rate reaches more than 45 percent; the inefficiency is 0%; the effect is obviously better than that of the eye cream prepared by the formulas 6 to 11 of the comparative example 1, and the eye cream prepared by the formula 1 of the example 3 has the best use effect, which shows that the repair eye cream provided by the invention has better effect of removing the pouches. And no cellulite was observed in all of the groups of volunteers except 7 eyes of the comparative 6 groups of volunteers during the test.

Fourth test example evaluation of skin elasticity-increasing and anti-wrinkle effects of eye cream

Number of test population: 110 female volunteer subjects aged 35-60 years, all volunteer subjects had healthy skin and no history of skin allergy, were selected, were assigned to the subject selection criteria, and were divided into 11 groups of 10 subjects, test 1-5 groups and comparative 1-6 groups, respectively, wherein test 1-5 groups used the eye creams prepared according to formulations 1-5 of example 3, and comparative 1-6 groups used the eye creams prepared according to formulations 6-11 of comparative example 1.

The using method comprises the following steps: after cleaning and refreshing the face in the morning and evening, a proper amount of the tested eye cream is taken and applied to the periphery of eyes, and the eye cream is evenly applied and massaged until being absorbed. The skin elasticity Cotomerma MPA580 host manufactured by Courage + Khazaka, Germany (CK, Germany) was used, the probe used was a Reviscometer RV600 elasto-fibrous tissue test probe, and the average was taken five times. Test area: the skin around the eyes. The blank values (R2, R5, R7) of the test area 30min after cleaning were measured, and then the subjects were prescribed to use the above test article every morning and evening to test the skin elasticity index R2, R5, R7 values of the test area after 0 week and 4 weeks, respectively (the negative pressure during the test was constant at 450 mbar).

Specification of indexes: the closer the skin elasticity indexes R2, R5, R7 are to 1, the better the elasticity of the skin.

R2＝Ua/Uf；R5＝Ur/Ue；R7＝Ur/Uf。

Uf — maximum amount of skin stretch.

Ue-the amount of skin stretch at 0.1 second after a constant negative pressure is applied to the skin, positioning the amount of stretch in the elastic portion.

Ur-after negative pressure is removed, the skin will quickly recover to the original state, and the values are also divided into elastic part value Ur, namely, after negative pressure is removed for 0.1 second, the recovery value and the viscoelastic part value, or the plastic part value, of the skin.

Ua-the recovery value of the skin from the elimination of the negative pressure to the next successive test of the skin surface plus the negative pressure.

The results are shown in Table 3 below.

TABLE 3 evaluation of skin elasticity-increasing and anti-wrinkle effects of the eye cream

The results show that R2, R5 and R7 of volunteer subjects respectively use the eye cream prepared by the formula 1-5 of the embodiment 3 for 4 weeks are obviously increased, the values of R2, R5 and R7 are close to 1, the skin elasticity index of the subjects is greatly increased and is obviously superior to the eye cream prepared by the formula 6-11 of the comparative example 1, and the using effect of the eye cream prepared by the formula 1 of the embodiment 3 is optimal, so that the eye cream has the effects of increasing skin elasticity, smoothening fine wrinkles and resisting aging. And no adipocyte appeared in all the volunteers of the other groups during the test, except that 3 of the volunteers of the comparative 6 groups showed a small amount of adipocyte on the eyes.

The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.

Claims (7)

1. The black eye removing, anti-wrinkle firming and repairing eye cream is characterized by comprising the following components in percentage by mass: 2-8% of glycerol, 1-3% of palmitic acid, 1-3% of squalane, 1-4% of cetostearyl alcohol, 101-3% of methyl gluceth-101, 0.5-4% of lecithin, 1-5% of caprylic/capric triglyceride, 6-12% of essential oil-containing nanoliposome, 0.5-4% of coffee cherokee extract, 0.5-4% of red globe ginger extract, 0.5-4% of rose apple leaf extract, 1-4% of undecylenoyl phenylalanine, 1-4% of acetyl chitosamine, 1-4% of aescin, 3-7% of plant fruit seed ultrafine powder, 1-6% of dextran sulfate sodium, 0.2-1% of polyacryloyl ammonium dimethyltaurate, 0.01-0.2% of phenoxyethanol, 0.01-0.2% of ethylhexylglycerol and the balance of deionized water;

the plant fruit seed ultrafine powder is composed of evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed in a mass ratio of 1 (1-2) to 0.5-1 to 1-3; the particle size of the plant fruit seed ultrafine powder is 0.2-1 mu m;

the essential oil-containing nano liposome comprises 20-30% of solid matters by mass, and the solid matters comprise the following components in percentage by mass: 5-10% of permanent flower essential oil, 5-10% of rock rose essential oil, 8-16% of vitamin E, 35-45% of hydrogenated soybean lecithin, 5-15% of cholesterol, 5-15% of beta-sitosterol and 8-804% of tween-D; the particle size of the essential oil-containing nano liposome is 100-150 nm.

2. The dark eye circle removing, anti-wrinkle and skin firming repair eye cream according to claim 1, characterized by comprising the following components by mass percent: 4-6% of glycerol, 1-2% of palmitic acid, 1-2% of squalane, 1-3% of cetostearyl alcohol, 101-3% of methyl gluceth, 1-3% of lecithin, 2-4% of caprylic/capric triglyceride, 8-12% of essential oil-containing nano liposome, 1-3% of small fruit coffee seed extract, 1-3% of red ball ginger extract, 1-3% of cattail leaf extract, 2-3% of undecylenoyl phenylalanine, 1-3% of acetyl chitosamine, 1-3% of aescin, 4-6% of plant fruit seed ultrafine powder, 2-5% of glucan sodium sulfate, 0.5-1% of polyacryl dimethyl ammonium taurate, 0.05-0.15% of phenoxyethanol, 0.05-0.15% of ethylhexylglycerol and the balance of deionized water.

3. The eye cream for removing dark circles, resisting wrinkles and tightening skin, repairing and protecting eyes as claimed in claim 1, wherein the plant fruit seed ultrafine powder is composed of evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed in a mass ratio of 1:1:0.5: 2.

4. The eye cream as claimed in claim 1, wherein the plant fruit seed micropowder is prepared by the steps of: mixing evening primrose seed, amur grape seed, purple alfalfa seed and buckwheat seed according to the formula ratio, and crushing to obtain seed mixed powder; and adding the seed and fruit mixed powder into an airflow mill pulverizer, performing airflow milling, allowing the fully milled micro powder to pass through gaps of a sorting wheel along with airflow, and allowing the insufficiently milled micro powder not to pass through, continuing the airflow milling until the particle size of the powder is 0.2-1 mu m, and collecting the plant fruit seed ultra-fine powder.

5. The eye cream for removing dark circles, resisting wrinkles and tightening the skin, repairing and protecting eyes as claimed in claim 1, wherein the essential oil-containing nanoliposome contains 20-30% by mass of solids, and the formulation of the solids comprises the following components by mass: 8% of permanent flower essential oil, 8% of rock rose essential oil, 14% of vitamin E, 40% of hydrogenated soybean lecithin, 12% of cholesterol, 12% of beta-sitosterol and 806% of tween-L.

6. The cream for removing dark circles, resisting wrinkles and firming skin according to claim 1, wherein the preparation of the nanoliposome containing essential oil comprises the following steps:

(1) weighing permanent flower essential oil, rock rose essential oil, vitamin E, hydrogenated soybean lecithin, cholesterol and beta-sitosterol according to a formula, adding the permanent flower essential oil, the rock rose essential oil, the vitamin E, the hydrogenated soybean lecithin, the cholesterol and the beta-sitosterol into absolute ethyl alcohol at the temperature of 40-60 ℃, and fully dissolving to obtain an oil phase;

(2) adding tween-80 with the formula amount into PBS buffer solution at the temperature of 40-50 ℃, slowly adding while stirring, and fully mixing to prepare a solution with the tween-80 of which the mass percent is 0.8-2.4%, so as to obtain a water phase;

(3) injecting the water phase into the oil phase by using an injector under the continuous stirring of a magnetic stirrer, fully mixing for 0.5-1 h, then carrying out rotary evaporation at the temperature of 28-32 ℃ and under the pressure of 0.1-1 MPa to remove ethanol, carrying out oscillation hydration reaction in a water bath at the temperature of 30-35 ℃ for 1-2 h to obtain liposome suspension, and adjusting the particle size of the liposome suspension to 100-150 nm by using an injector type filter to obtain the essential oil-containing nano liposome.

7. A method for preparing the dark-eye-circle-removing, anti-wrinkle and skin-firming repair eye cream of claim 1, comprising the steps of:

(1) adding deionized water, glycerol, acetyl chitosamine, plant fruit seed ultrafine powder, dextran sulfate sodium and polyacryl dimethyl ammonium taurate into a water phase pot according to the formula amount, heating to 70-75 ℃, and uniformly stirring to obtain a water phase;

(2) adding palmitic acid, squalane, cetostearyl alcohol, methyl gluceth-10, lecithin and caprylic/capric triglyceride into an oil phase pot according to the formula ratio, heating to 70-75 ℃, and uniformly stirring to obtain an oil phase;

(3) pumping the water phase into a vacuum emulsifying machine, starting stirring and vacuum, sucking the oil phase into the emulsifying machine under the conditions of 80-85 ℃, vacuum degree of-0.06 to-0.04 MPa and stirring rotation speed of 60-80 r/min to obtain a mixed solution, and homogenizing for 10-15 min at 2000-3000 r/min;

(4) after homogenizing, keeping the vacuum degree and stirring speed unchanged, cooling to 40-45 ℃, adding the essential oil-containing nano liposome, the small fruit coffee seed extract, the red globe ginger extract, the syzygium jambos leaf extract, the undecylenoyl phenylalanine, the aescin, the phenoxyethanol and the ethylhexyl glycerin, continuously stirring uniformly, cooling to room temperature, discharging, and filling to obtain the eye cream for removing black eye circles, resisting wrinkles and tightening the skin.