CN105055480A - Curry plant essential oil nano-liposome antibacterial agent, and preparation method thereof - Google Patents
Curry plant essential oil nano-liposome antibacterial agent, and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of antiseptic and medicinal preparation or cosmetic, and more specifically relates to a curry plant essential oil nano-liposome antibacterial agent, and a preparation method thereof. The curry plant essential oil nano-liposome antibacterial agent is prepared from curry plant essential oil, soya bean lecithin, cholesterol, a surfactant, chitosan, and gelatin. The preparation method comprises following steps: curry plant essential oil, soya bean lecithin, and cholesterol are mixed with an organic solvent; an obtained mixture is subjected to reduced pressure drying so as to obtain a smooth thin film; a water-phase medium and the surfactant are added for dissolving the smooth thin film, and an obtained product is subjected to ultrasonic treatment so as to obtain an emulsion; the emulsion is mixed with chitosan and gelatin respectively, and is subjected to uniform homogenizing; an obtained mixed material is subjected to centrifugation and filtering with a microfiltration membrane so as to obtain the nano-grade liposome. The preparation method is high in repeatability; encapsulation efficiency of the curry plant essential oil multilayer nano-liposome can be as high as 94.8%; product morphology is complete; particle size is uniform; and the curry plant essential oil nano-liposome antibacterial agent possesses excellent stability and antibacterial properties.
Description
Technical field
The invention belongs to antibacterial and pharmaceutical preparation or cosmetic field, be specifically related to a kind of Italian cured chrysanthemum essence oil nanometer liposome antibacterial and preparation method thereof.
Background technology
The cured chrysanthemum of Italy, have another name called Curry Plantrd, originate in eastern region, Mediterranean, belong to the one of Compositae, its quintessence oil is widely used in fragrance industry and aromatotherapy; Its main component has neryl acetate, curcumene, nerol, firpene, has extraordinary antiinflammatory, antiallergic, antioxidation, radioprotective, spasmolytic, antiviral, convergence, sterilization, soft skin, promotes the effects such as cell regeneration, antifungal, antifungal, calmness; Patent both at home and abroad about the cured chrysanthemum of Italy cans be counted on one's fingers: Chinese patent CN102273492A discloses a kind of sanitizer formulations containing Italian Helichrysum; Korean Patent KR101173412B1 discloses a kind of compositions that can be used for skin wrinkle resisting containing Italian cured chrysanthemum; French Patent (FRP) FR2965729A1 discloses a kind of compositions being used for the treatment of skin allergy containing Italian Helichrysum; French Patent (FRP) FR2953402A1 disclose a kind of containing Italian Helichrysum for skin repair, and treatment hematoma, healing and the compositions of inflammation; Korean Patent KR20110023257A discloses a kind of compositions of the promoted skin regeneration containing Italian Helichrysum; Korean Patent KR20030046949A discloses a kind of anti-inflammatory composition containing Italian Helichrysum; French Patent (FRP) FR2811899A1 discloses a kind of antitumor, antiviral composition containing Italian Helichrysum.
The main cause of the cured chrysanthemum refined-oil development application of current restriction Italy has: quintessence oil is volatile, and its main component is is easily scattered and disappeared; The easy oxidized decomposition of quintessence oil.So be made into nanometer liposome, use microcapsule technology effectively can improve the stability of quintessence oil, be easy to deposit, extend storage period.
Liposome is the hydrophilic vesicle be made up of phospholipid bilayer, scattered in water, and the main component phospholipid of liposome is one of main constituent of cell membrane, and good biocompatibility, toxicity is little, and safety is high.Cured for Italy chrysanthemum quintessence oil is embedded in liposome, not only can not damage the main active of the cured chrysanthemum quintessence oil of Italy, and can make that the active component in quintessence oil and external environment are isolated to come, reduce its volatilization, degree of oxidation, extend storage period, finally, the small-size effect of nanometer liposome and skin effect make it have good dissolubility and diffusion permeability, can be widely used in food, medicine, cosmetics.
At present still not about the report of Italy's cured chrysanthemum essence oil nanometer liposome, it is large to there is product cut size in traditional method for preparing lipidosome, skewness, the shortcomings such as poor stability, and due to the slow releasing function of liposome, the unilamellar liposome shelf-life of wrapping up Italian cured chrysanthemum quintessence oil is limited; Italy's cured chrysanthemum quintessence oil multi-layer nano liposome antibacterial of a kind of multilamellar of invention, selects chitosan and gelatin to make the much higher layer liposome of stability, effectively improves utilization rate and the storage period of quintessence oil.
Summary of the invention
Italy's cured chrysanthemum essence oil nanometer liposome that the object of the invention is openly a kind of high stability and preparation method thereof, by cured for Italy chrysanthemum quintessence oil is wrapped in multi-layer nano liposome, to realize reducing the volatilization of Italian cured chrysanthemum quintessence oil in application, thus reduce the waste of Italian cured chrysanthemum quintessence oil, reach the object of long acting antibiotic and efficiency utilization.
A kind of Italian Helichrysum essence oil nanometer liposome, Italian Helichrysum quintessence oil is wrapped in phospholipid bilayer, it is characterized in that: phospholipid bilayer is ground floor nanometer liposome, and be also provided with second layer nanometer liposome, the second layer is made up of chitosan.
Further, described one Italy Helichrysum essence oil nanometer liposome, is characterized in that: be also provided with third layer nanometer liposome, third layer is made up of gelatin.
Further, in second layer nanometer liposome, the concentration of chitosan is 0.2mg/mL.
Further, in third layer nanometer liposome, the concentration of gelatin is 0.4mg/mL.
The present invention is by the cured chrysanthemum quintessence oil of Italy, and soybean lecithin, cholesterol, surfactant, Italian cured chrysanthemum essence oil nanometer liposome made by chitosan and gelatin.
Soybean lecithin, cholesterol are that the group forming liposome wants composition, and cholesterol all has the effect regulating membrane fluidity, so investigate the proportioning change both it, on parameters such as the liposomal dispersion formed with or without impact; Surfactant increases liposome stability; Italy's Helichrysum essential oil concentration is determined according to envelop rate; The pH of Acetate Solution is in order to optimal dissolution chitosan; Chitosan and gelatin can increase stability.
Preparation method of the present invention is by Italian Helichrysum quintessence oil, soybean lecithin, cholesterol is mixed in organic solvent, evaporated under reduced pressure forms smooth thin film, add mixed solution dissolving films that aqueous media and surfactant form and ultrasonic become breast, centrifugal after get supernatant liquid and filter and obtain monolayer Italy Helichrysum essence oil nanometer liposome, it is characterized in that: monolayer Italy's Helichrysum essence oil nanometer liposome and chitosan solution are stirred, by centrifugal and filtering with microporous membrane, obtaining particle diameter is nano level bilayer Italian Helichrysum essence oil nanometer liposome.
Further, Italian for bilayer Helichrysum essence oil nanometer liposome and gelatin solution are stirred, by centrifugal and filtering with microporous membrane, obtaining particle diameter is nano level multilamellar Italy Helichrysum essence oil nanometer liposome.
Further, the mass ratio of soybean lecithin of the present invention and cholesterol is 5:1; The mass ratio of surfactant, Italian Helichrysum quintessence oil and cholesterol is: 1:5:4, can obtain the highest envelop rate under this condition.
Further, surfactant is PVP, and in mixed solution, the concentration of PVP is 1.0mg/mL.
The volume ratio of monolayer Italy's Helichrysum essence oil nanometer liposome and chitosan solution is 1:10; The volume ratio of double-deck Italian Helichrysum essence oil nanometer liposome and gelatin solution is 1:10.
Organic solvent described in the present invention is chloroform.
Aqueous media used in the present invention is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0.
Described chitosan solution is the Acetate Solution of chitosan, and concentration is 0.2mg/mL, and Acetate Solution is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0, preferably 3.6, and can optimal dissolution chitosan.
Institute's gelatine solution is the Acetate Solution of gelatin, and concentration is 0.4mg/mL, and Acetate Solution is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0.
In the present invention, the ground floor of liposome is phospholipid bilayer, i.e. artificial cell rete.
In the present invention, the second layer of liposome is made up of chitosan, and concentration is 0.2mg/mL.
In the present invention, the third layer of liposome is made up of gelatin, and concentration is 0.4mg/mL.
Accompanying drawing explanation
Fig. 1 is the envelop rate of Italian cured chrysanthemum essence oil nanometer liposome.
Fig. 2 is particle diameter and the polydispersity coefficient PDI of Italian cured chrysanthemum essence oil nanometer liposome.
Fig. 3 is the cured chrysanthemum essence oil nanometer liposome fluorescence microscopy figure of multilamellar Italy.
Fig. 4 is the cured chrysanthemum essence oil nanometer liposome atomic force microscopy figure of multilamellar Italy.
The cured chrysanthemum essence oil nanometer liposome of Fig. 5 multilamellar Italy is to colibacillary anti-microbial property.
The cured chrysanthemum essence oil nanometer liposome of Fig. 6 multilamellar Italy is to the anti-microbial property of staphylococcus aureus.
Table 1 is the Zeta potential of Italian cured chrysanthemum essence oil nanometer liposome.
Detailed description of the invention
By example below, the specific embodiment of the present invention is described, but protection content of the present invention, be not only confined to this.
the envelop rate of the cured chrysanthemum essence oil nanometer liposome of embodiment 1 multilamellar Italy
1 experiment material
Soybean lecithin; BR; Chemical Reagent Co., Ltd., Sinopharm Group.
Cholesterol; AR; Chemical Reagent Co., Ltd., Sinopharm Group.
Chloroform; AR; Chemical Reagent Co., Ltd., Sinopharm Group.
The cured chrysanthemum quintessence oil of Italy; AR; France florihana quintessence oil.
PVP; GR; Chemical Reagent Co., Ltd., Sinopharm Group.
Chitosan; BR; Chemical Reagent Co., Ltd., Sinopharm Group.
Gelatin; BR; Chemical Reagent Co., Ltd., Sinopharm Group.
2 experimental techniques
1) preparation of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy
1. take 1g soybean lecithin, the cured chrysanthemum quintessence oil of Italy of 0.2g cholesterol and 250mg, adds 50mL chloroform and makes it dissolve.
2. in Rotary Evaporators, be evaporated to solvent evaporate to dryness, evaporating temperature is 10 ~ 30 DEG C, and round-bottomed flask inwall can form smooth thin film; Then products obtained therefrom is put into vacuum drying oven, 30 DEG C, drying 24 hours under vacuum state.
3. the PVP taking 0.05g, in 50ml acetate buffer, spreads under Ultrasonic Conditions, is then added in round-bottomed flask by the acetate buffer of PVP and carries out aquation under Ultrasonic Conditions.
4. by the mixed liquor after aquation in cell micronizing instrument with the 10s that works, the frequency of gap 5s pulverizes 30min.
5. products obtained therefrom is carried out centrifugal, 4000rpm, 15min, get supernatant liquid.
6. being filtered by gained liquid 0.22 μm of filter membrane, obtain filtrate, is the cured chrysanthemum essence oil nanometer liposome of monolayer Italy.
2) preparation of double-deck Italian cured chrysanthemum essence oil nanometer liposome
1. according to the preparation method of the cured chrysanthemum essence oil nanometer liposome of above-mentioned monolayer Italy, preparation is containing the monolayer nanometer liposome of the cured chrysanthemum quintessence oil of 250mg Italy.
2. by the cured chrysanthemum essence oil nanometer liposomal dispersion of monolayer Italy mix homogeneously in the Acetate Solution containing 0.2mg/mL chitosan; The volume ratio 1:10 of the Acetate Solution of the monolayer cured chrysanthemum essence oil nanometer liposome of Italy and chitosan.
3. by gained mixed liquor in cell micronizing instrument with the 10s that works, the frequency of gap 5s pulverizes 30min.
4. products obtained therefrom is carried out centrifugal, 4000rpm, 15min, get supernatant liquid.
5. being filtered by gained liquid 0.22 μm of filter membrane, obtain filtrate, is the Italian cured chrysanthemum essence oil nanometer liposome of bilayer.
3) preparation of the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy
1. according to the preparation method of the Italian cured chrysanthemum essence oil nanometer liposome of above-mentioned bilayer, preparation is containing the double-layer nanometer liposome of the cured chrysanthemum quintessence oil of 250mg Italy.
2. Italian for bilayer cured chrysanthemum nanometer liposome is dispersed in the Acetate Solution containing 0.4mg/mL gelatin and makes its mix homogeneously; The volume ratio of the Acetate Solution of double-deck Italian cured chrysanthemum nanometer liposome and gelatin is 1:10.
3. by gained mixed liquor in cell micronizing instrument with the 10s that works, the frequency of gap 5s pulverizes 30min.
4. products obtained therefrom is carried out centrifugal, 4000rpm, 15min, get supernatant liquid.
5. being filtered by gained liquid 0.22 μm of filter membrane, obtain filtrate, is the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy.
4) mensuration of envelop rate
With the Italian cured chrysanthemum quintessence oil of dehydrated alcohol dilution, stepwise dilution becomes concentration to be respectively 0.1,0.2,0.4,0.6, the standard solution of 0.8mg/mL, then, draw 1 μ L standard solution respectively and carry out GC-MS analysis, automatic integration is carried out to the spectrum peak area of its main component neryl acetate, draw neryl acetate peak area-Italian cured chrysanthemum essential oil concentration standard curve, first the cured chrysanthemum essence oil nanometer liposomal samples of 1mL Italy is got, 13500rpm, centrifugal 3h, outwells supernatant.Then 1mL ethanol demulsifier is added, ultrasonic 3h, last with the centrifugal 15min of the rotating speed of 10000rpm, get supernatant, analyze for GC-MS, the liposomal samples prepared, carries out automatic integration to the face, peak of quintessence oil main constituent, again according to the standard curve drawn in step 1, calculate the content of plants essential oil in liposome.
Then:
.
The envelop rate of 3 Italian cured chrysanthemum essence oil nanometer liposomees
Envelop rate is the most important index evaluating Liposomal formulation quality, is also the key that can liposome play the features such as, low toxicity efficient compared with ordinary preparation; As seen from Figure 1, the envelop rate of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy is 42.1%, the envelop rate of double-deck Italian cured chrysanthemum essence oil nanometer liposome is 64.5%, the envelop rate of the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy is maximum, be 94.4%, therefore prepare the envelop rate that the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy can significantly improve liposome.
the particle diameter of the cured chrysanthemum essence oil nanometer liposome of embodiment 2 multilamellar Italy and polydispersity coefficient PDI
1 experiment material
1. the cured chrysanthemum essence oil nanometer liposome of monolayer Italy.
2. double-deck Italian cured chrysanthemum essence oil nanometer liposome.
3. the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy.
2 experimental techniques
Produce with Brooker Hai Wen instrument company of the U.S., model is that the high concentration laser particle analyzer of BI-9000 measures the particle diameter of Italian cured chrysanthemum essence oil nanometer liposome and polydispersity coefficient PDI value, institute's test sample product are put into sample cell and directly measured.
The particle diameter of 3 Italian cured chrysanthemum essence oil nanometer liposomees and polydispersity coefficient PDI
Polydispersity coefficient PDI directly reflects the stability of Italian cured chrysanthemum essence oil nanometer liposome, and be therefore Primary Reference index, the PDI of liposome belongs to best in 0 ~ 0.3 scope, poor in 0.3 ~ 0.7 scope, but can accept, as PDI>0.8, not consider; As shown in Figure 2, the particle diameter of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy is 177.4nm, PDI is 0.322, the particle diameter of double-deck Italian cured chrysanthemum essence oil nanometer liposome is 204.5nm, PDI is 0.287, and the particle diameter of the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy is 282.6nm, PDI is 0.173; The polydispersity coefficient PDI of the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy is minimum, therefore prepares the stability that the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy can significantly improve liposome.
the Zeta potential of the Italian cured chrysanthemum essence oil nanometer liposome of embodiment 3
1 experiment material
1. the cured chrysanthemum essence oil nanometer liposome of monolayer Italy.
2. double-deck Italian cured chrysanthemum essence oil nanometer liposome.
3. the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy.
2 experimental techniques
Measure with the potentiometer that the model that Malvern Instr Ltd. of Britain produces is ZetasirernanoZSZeta, directly liposomal samples to be measured is put into potentiometer and measure.
The Zeta potential of 3 Italian cured chrysanthemum essence oil nanometer liposomees
The Zeta potential of the Italian cured chrysanthemum essence oil nanometer liposome of table 1
| The cured chrysanthemum essence oil nanometer liposome of Italy | Zeta potential |
| Monolayer | -31.3mV |
| Double-deck | -35.9 mV |
| Multilamellar | -52.3mV |
Zeta potential also directly can reflect the stability of Italian cured chrysanthemum essence oil nanometer liposome, therefore be also Primary Reference index, the larger explanation liposome of the absolute value more stability of the Zeta potential of liposome, the absolute value of Zeta potential belongs to unstable, the liposome comparatively stability when being greater than 30 in 0 ~ 30 scope; As shown in table 1, three kinds of Italy's cured chrysanthemum essence oil nanometer liposomees are all electronegative, the Zeta potential of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy is-31.3mV, it is more stable that its absolute value is greater than 30 liposomees, the Zeta potential of double-deck Italian cured chrysanthemum essence oil nanometer liposome is-35.9mV, and it is more stable that its absolute value is greater than 30 liposomees, and the Zeta potential of the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy is-52.3mV, its maximum absolute value, liposome is the most stable.
the fluorescence microscope of the cured chrysanthemum essence oil nanometer liposome of embodiment 4 multilamellar Italy
1 experiment material
The cured chrysanthemum essence oil nanometer liposome of multilamellar Italy.
2 experimental techniques
With Leca instrument company produce model be the fluorescence microscope of TCS-SP5, directly liposomal samples to be measured is put into fluorescence microscope and observes.
Fluorescence microscope sample-pretreating method:
(1) preparation (A liquid) of the cured chrysanthemum essence oil nanometer liposomal samples of multilamellar Italy: get the cured chrysanthemum quintessence oil liposome of 1mL Italy, 0.5mL methanol and the mixing of 0.5mL chloroform.
(2) preparation (B liquid) of fluorescent dye DIL: the DIL of 0.1mL is dissolved in the dichloromethane of 0.1mL.
(3) get A liquid 0.5mL and B liquid 50 μ L and put into the mixing of little centrifuge tube, concussion evenly.
(4) above-mentioned mixing material is put into vacuum drying oven, a dry night.
(5) get dry centrifuge tube, add 0.5mL ultra-pure water, shake 30min on the oscillator.
(6) room temperature places 3h.
(7) drop on microscope slide and observe.
The fluorescence microscope of the 3 cured chrysanthemum essence oil nanometer liposomees of multilamellar Italy
The microphotograph photographed as can be seen from above fluorescence microscope, after liposome dyeing, presents circle, disperses more even.
the atomic force microscope observation of the cured chrysanthemum essence oil nanometer liposome of embodiment 5 multilamellar Italy
1 experiment material
The cured chrysanthemum essence oil nanometer liposome of multilamellar Italy.
2 experimental techniques
With Agilent Technologies of the U.S. produce model be the atomic force microscope of Agilent5500, directly liposomal samples to be measured is put into atomic force microscope to observe, atomic force pre-treating method gets plants essential oil liposomal samples 10 μ L to drop in 10min on mica sheet, then the liquid on surface is absorbed with liquid-transfering gun, drip 10 μ L ultra-pure water 30s again, repeated washing 3 times, ventilation leaves standstill 3h, observes under being positioned over atomic force microscope.
The atomic force microscope observation of the 3 cured chrysanthemum essence oil nanometer liposomees of multilamellar Italy
The microphotograph photographed as can be seen from above atomic force microscope, liposome presents circle, disperses more even.
the anti-microbial property of the cured chrysanthemum essence oil nanometer liposome of embodiment 6 multilamellar Italy
1 experiment material
1. the monolayer cured chrysanthemum essence oil nanometer liposome of Italy (preserving 7 days, 30 days, 60 days, 90 days).
2. double-deck Italian cured chrysanthemum essence oil nanometer liposome (preserving 7 days, 30 days, 60 days, 90 days).
3. the multilamellar cured chrysanthemum essence oil nanometer liposome of Italy (preserving 7 days, 30 days, 60 days, 90 days).
2 experimental techniques
Adopt the method for plate culture count, with escherichia coli (
escherichiacoli) and staphylococcus aureus (
staphylococcusaureus) measure the remaining bacterium number of Italian cured chrysanthemum essence oil nanometer liposome for pattern bacterium, by escherichia coli and S. aureus Inoculate in fluid medium, be placed in respectively gas bath shaking table 37 DEG C, shake cultivation 24 ~ 48h under 150rpm condition, obtain the antibacterial of exponential phase, get in the test tube that the escherichia coli that are in logarithmic (log) phase in right amount and staphylococcus aureus add containing a certain amount of sterile phosphate buffer respectively that (bacteria concentration is about 10
5~ 10
6cfu/mL), and then in test tube, add the various Italy cured chrysanthemum essence oil nanometer liposome that concentration is 10%, separately get two test tubes respectively containing above two kinds of bacterium simultaneously and also add equivalent sterilized water (not adding Italian cured chrysanthemum essence oil nanometer liposome) wherein in contrast, each test tube is all placed in gas bath shaking table at 37 DEG C, concussion reaction 24h under 150rpm condition, appropriate culture fluid of getting respectively at different time points carries out ten times of gradient dilutions to suitable concentration, then pipetting 100 μ L diluents drips on sterile solid plating medium, coating evenly, put into 37 DEG C of constant temperature and humidity incubators afterwards and be inverted cultivation, plate count is carried out after 24 ~ 48h, thus to evaluating the antibacterial activity of each Italy cured chrysanthemum essence oil nanometer liposome, do three repetitions, results averaged.
The anti-microbial property of the 3 cured chrysanthemum essence oil nanometer liposomees of multilamellar Italy
The change of the antibacterial activity of Italy's cured chrysanthemum essence oil nanometer liposome of different storage life also can reflect the stability of liposome indirectly, therefore carried out anti-microbial property evaluation to preservation 7 days, the various Italy cured chrysanthemum essence oil nanometer liposome of 30 days, 60 days, 90 days, result as shown in Figure 5, Figure 6; Holding time is when being 7 days, the antibacterial activity of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy, double-deck Italian cured chrysanthemum essence oil nanometer liposome, the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy is all identical, all shows good antibacterial effect to escherichia coli and staphylococcus aureus; Holding time, when being 30 days, the antibacterial effect of the cured chrysanthemum essence oil nanometer liposome of monolayer Italy obviously reduced and the cured chrysanthemum essence oil nanometer liposome of double-deck Italian cured chrysanthemum essence oil nanometer liposome and multilamellar Italy all shows good antibacterial effect; Holding time be 60 days and 90 days time, the cured chrysanthemum essence oil nanometer liposome of monolayer Italy does not show antibacterial effect, the antibacterial effect of double-deck Italian cured chrysanthemum essence oil nanometer liposome obviously reduces, and the cured chrysanthemum essence oil nanometer liposome of multilamellar Italy keeps good antibacterial effect always.
Claims (10)
1. an Italian Helichrysum essence oil nanometer liposome, Italy's Helichrysum quintessence oil is wrapped in phospholipid bilayer, it is characterized in that: phospholipid bilayer is ground floor nanometer liposome, also be provided with second layer nanometer liposome, the second layer is made up of chitosan, to improve envelop rate, stability and anti-microbial property.
2. a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 1, is characterized in that: be also provided with third layer nanometer liposome, third layer is made up of gelatin, improves envelop rate, stability and anti-microbial property further.
3. a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 1, is characterized in that: in second layer nanometer liposome, the concentration of chitosan is 0.2mg/mL.
4. a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 2, is characterized in that: in third layer nanometer liposome, the concentration of gelatin is 0.4mg/mL.
5. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 1, by Italian Helichrysum quintessence oil, soybean lecithin, cholesterol is mixed in organic solvent, evaporated under reduced pressure forms smooth thin film, add mixed solution dissolving films that aqueous media and surfactant form and ultrasonic become breast, get supernatant liquid filtration after centrifugal and obtain monolayer Italy Helichrysum essence oil nanometer liposome, it is characterized in that: monolayer Italy's Helichrysum essence oil nanometer liposome and chitosan solution are stirred, by centrifugal and filtering with microporous membrane, obtaining particle diameter is nano level bilayer Italian Helichrysum essence oil nanometer liposome.
6. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 5, it is characterized in that: further, Italian for bilayer Helichrysum essence oil nanometer liposome and gelatin solution are stirred, by centrifugal and filtering with microporous membrane, obtaining particle diameter is nano level multilamellar Italy Helichrysum essence oil nanometer liposome.
7. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 5, is characterized in that: the mass ratio of soybean lecithin and cholesterol is 5:1; The mass ratio of surfactant, Italian Helichrysum quintessence oil and cholesterol is: 1:5:4, can obtain the highest envelop rate under this condition; Described organic solvent is chloroform.
8. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 5, it is characterized in that: surfactant is PVP, in mixed solution, the concentration of PVP is 1.0mg/mL; Aqueous media used is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0.
9. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 5, is characterized in that: the volume ratio of monolayer Italy's Helichrysum essence oil nanometer liposome and chitosan solution is 1:10; Described chitosan solution is the Acetate Solution of chitosan, and concentration is 0.2mg/mL, and Acetate Solution is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0, preferably 3.6, and can optimal dissolution chitosan.
10. the preparation method of a kind of Italian Helichrysum essence oil nanometer liposome as claimed in claim 6, is characterized in that: the volume ratio of double-deck Italian Helichrysum essence oil nanometer liposome and gelatin solution is 1:10; Institute's gelatine solution is the Acetate Solution of gelatin, and concentration is 0.4mg/mL, and Acetate Solution is the acetate buffer solution according to Chinese Pharmacopoeia 2000 editions standard preparation, pH value 3.5 ~ 4.0.
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| CN108969444A (en) * | 2018-09-19 | 2018-12-11 | 陈洁珍 | One kind removing black eye, crease-resistant Firm anti-age corrective eye treatment cream and preparation method thereof |
| CN108969444B (en) * | 2018-09-19 | 2021-02-02 | 青岛生康盛生物科技有限公司 | Black eye removing, anti-wrinkle firming and repairing eye cream and preparation method thereof |
| CN115386643A (en) * | 2022-08-18 | 2022-11-25 | 宁波赛缪斯生物科技有限公司 | Method for customizing skin care product based on anti-inflammatory gene and skin care product |
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Application publication date: 20151118 |