CN103876982A - Nano-liposome emulsion and preparation method thereof - Google Patents
Nano-liposome emulsion and preparation method thereof Download PDFInfo
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- CN103876982A CN103876982A CN201410110768.4A CN201410110768A CN103876982A CN 103876982 A CN103876982 A CN 103876982A CN 201410110768 A CN201410110768 A CN 201410110768A CN 103876982 A CN103876982 A CN 103876982A
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Abstract
The invention relates to nano-liposome emulsion. A seaweed extract is packaged inside a nano-liposome. The nano-liposome emulsion contains 10wt%-20wt% of the seaweed extract, 10wt%-20wt% of lecithin, 1wt%-3wt% of a propping agent, 0.01wt%-0.1wt% of an antioxidant, 10wt%-20wt% of a solubilizer and 10wt%-20wt% of a stabilizing agent. According to the invention, the nano-liposome emulsion inside which the seaweed extract is packaged is prepared at first and has a skin regeneration function, and a product has relatively good water solubility and can be randomly mixed with a skin care preparation, so that a functional cosmetic is relatively convenient and efficient in preparation. A liposome packaging process can be used for improving the stability of the seaweed extract and the compatibility of the seaweed extract in a cosmetic system; the carrying function of the liposome is beneficial for the seaweed extract to go deep into a deep layer of the skin, thus a sensitization response caused by direct contact between the seaweed extract and the epidermic cells of the skin is reduced or avoided, and the seaweed extract is also fully takes the effects.
Description
Technical field
The present invention relates to cosmetics technical field, particularly relate to a kind of nano-lipid precursor emulsion, and the preparation method of this emulsion.
Background technology
Along with the decline of people's surrounding enviroment quality, the quickening of rhythm of life and the aggravation of operating pressure, our outermost layer armour-skin suffers more and more many external damages.Damaged or imperfect, the pigment of epidermis come off or the problem such as damaged of the hypertrophy of calm, cicatrix and formation and cutaneous nerve system, tiny blood vessels highlights day by day.In the face of this sight, people also progressively turn to beauty treatment reparation from simple covering to the requirement of cosmetics function.
Sargassum is as one of the earth the most ancient known plant, wide in variety, output is abundant, is to be a kind ofly widely grown in low in ocean and to wait cryptogam.The bioactive substance such as sulfated polysaccharide, superoxide dismutase that its unique living environment is given, and the precious composition such as abundant trace element, somatomedin, ocean ferment, there is antioxidation, anti-inflammation and sterilization, anti-ultraviolet and the health care function of waiting for a long time, the generation of energy trigger cell somatomedin, promote the growth of various cells, effectively repair the cell and the tissue that are undermined degeneration, play activating cell, desalination epidermis microgroove, conditioning sensitivity skin quality, promotes skin water retaining function.
In the Application and Development process of cosmetics, because the complexity of self component is with unique, there is certain skin allergic phenomena in Sargassum extract at present.In addition; compatibility problems in Sargassum extract use procedure, also needs effective solution, should meet the stability that does not affect other compositions and whole system in cosmetics; protect again the unique effects of Sargassum extract self, avoid the impact of other compositions in cosmetics.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of nano-lipid precursor emulsion, to solve the deficiency of Sargassum extract application in the preparation of existing cosmetics, can avoid or reduce Sargassum extract and epiderm skin cell and directly contact the sensitization of generation.
A kind of nano-lipid precursor emulsion, parcel Sargassum extract (English name: Algae Extract) in nanometer liposome.
A kind of nano-lipid precursor emulsion as above, further, contains: Sargassum extract 10～20wt%, lecithin 10～20wt%, proppant 1～3wt%, antioxidant 0.01～0.1wt%, solubilizing agent 10～20wt%, stabilizing agent 10～20wt%.
Preferably, the particle diameter of described nanometer liposome is at 10nm～100nm.
Preferably, described lecithin is selected from one or more in soybean lecithin, Ovum Gallus domesticus Flavus lecithin and hydrolecithin.
Preferably, described proppant is selected from one or more in cholesterol, stigmasterol and cupreol.
Preferably, described antioxidant is selected from one or more in vitamin E, vitamin C and superoxide dismutase.
Preferably, described solubilizing agent is selected from one or more in Tween 80, Macrogol 4000 and Fatty acid macrogolglycerides.
Preferably, described stabilizing agent is one or more in glycerol, propylene glycol and butanediol.
The preparation method of nano-lipid precursor emulsion as mentioned above, comprises the following steps:
Step 1, takes formula ratio Sargassum extract, lecithin, proppant, solubilizing agent, antioxidant, mixes and be stirred well to even oil phase under 55～60 ℃ of temperature conditions, is incubated 6～12min;
Step 2, the stabilizing agent that takes formula ratio is dissolved in pure water, is warming up to 55～60 ℃, stirs as even water insulation 6～12min;
Step 3, carries out premix in water prepared by oil phase suction step 2 prepared by step 1, uses homogenizer high-speed homogenization 15～25min, at 55～60 ℃ of insulation 6～12min, makes colostric fluid;
Step 4, moves on to colostric fluid in high pressure homogenizer, carries out high pressure homogenize circular treatment under 80～120MPa pressure; In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, obtain nano-lipid precursor emulsion.
Preferably, the ethanol extract that in the present invention, Sargassum extract is Sargassum, composition comprises saccharide, alkaloid, steroid saponin, flavone and lactone etc., physiology and the pharmacologically active of have conditioning, repairing.For example Sargassum polysaccharides has moisturizing, antibacterial and antioxidant activity; That flavone compound has is anticorrosion, antioxidation and unique pharmacologically active; Steroid saponin has the effect such as hemostasis, adjusting.
The present invention prepares the nano-lipid precursor emulsion of parcel Sargassum extract first, and it has skin repair function, and this liposome emulsion has the following advantages:
1, the liposome particle diameter of nano-lipid precursor emulsion is below 100nm, and emulsion appearance is transparent or semitransparent, constant product quality and there is good water solublity, can with any interworking of skin care formulation, make the configuration of effect cosmetic convenient, efficient.
2, liposome technology improves Sargassum extract stability, and compatibility in cosmetics series; Liposome carrying function is beneficial to Sargassum extract and gos deep into deep skin, reduces or avoids directly contacting with epiderm skin cell the sensitivity response causing, and also can make Sargassum extract give full play to effect.
3, the slow release characteristic of liposome and the repair function of self, can effectively strengthen and extend beauty treatment maintenance good result.
4, liposome technology is the closed nanoscale vesicle that utilizes natural phospholipid compounds to prepare to have bilayer structure.The structure of this similar cell membrane can realize the effective parcel to water solublity and fat-soluble active substance, active substance is had to the functions such as protection, delivery and slow release.Liposome is prepared the basic support that main material-natural phospholipid used is biological cell membrane, has very strong affinity interaction with cell, can strengthen mobility and the permeability of cell, promotes growth and the reparation of epidermis cell, strengthens the metabolic activity of cell.Therefore; utilize liposome technology effectively to wrap up Sargassum extract, by class film nano vesicle structure, Sargassum extract is protected and delivered to deep skin, guarantee the integrity of its composition; avoid directly contacting caused sensitization with epidermis, also can realize skin and efficiently nurse effect.
Accompanying drawing explanation
Fig. 1 is the results of grain size analysis figure of the nano-lipid precursor emulsion prepared of the embodiment of the present invention 2.
Fig. 2 is the morphology characterization result figure of the nano-lipid precursor emulsion prepared of the embodiment of the present invention 2.
The specific embodiment
Below in conjunction with the specific embodiment, principle of the present invention and feature are described, example, only for explaining the present invention, is not intended to limit scope of the present invention.
In following examples, Sargassum extract used can be prepared by the following method, gets fresh Sargassum and cleans up, and naturally dries in the shade at ambient temperature.The Sargassum that pulverization process has been dried, adds 95% ethanol of 5 times of raw materials quality, soaks 10-12h.Heating and refluxing extraction 3 times, each 2 hours, filters merge extractive liquid.In extracting solution, add the activated carbon decolorizing of 2% quality to process 1 hour.Remove by filter active carbon, through distilling under reduced pressure concentrated filtrate, obtain Sargassum ethanol extraction extractum.
Embodiment 1
1, the cooling ultrasonic dissolution assisting of vitamin E that takes 20.0g Sargassum extract, 10.0g soybean lecithin, 1.0g cholesterol and 0.01g, adds 10.0g Tween 80, is warming up to 55-60 ℃, and to be stirred well to be evenly oil phase, insulation 10min.
2, take 15.0g glycerol, be added in 44.0g pure water, be warming up to 55-60 ℃, stir as water, insulation 10min.
3, will in oil phase material suction water, carry out premix, adjust rotating speed 10000r/min high-speed homogenization 20min, at 55-60 ℃ of insulation 10min, make colostric fluid.
4, colostric fluid is moved on in high pressure homogenizer, under 80MPa pressure, carry out high pressure homogenize circular treatment.In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, must be coated the skin repair function nano liposome emulsion of Sargassum extract.
Embodiment 2
1, the cooling ultrasonic dissolution assisting of vitamin E that takes 10.0g Sargassum extract, 15.0g soybean lecithin, 2.0g cholesterol and 0.1g, adds 10.0g Tween 80, is warming up to 55-60 ℃, and to be stirred well to be evenly oil phase, insulation 10min.
2, take 20.0g glycerol, be added in 42.9g pure water, be warming up to 55-60 ℃, stir as water, insulation 10min.
3, will in oil phase material suction water, carry out premix, adjust rotating speed 10000r/min high-speed homogenization 20min, at 55-60 ℃ of insulation 10min, make colostric fluid.
4, colostric fluid is moved on in high pressure homogenizer, under 100MPa pressure, carry out high pressure homogenize circular treatment.In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, must be coated the skin repair function nano liposome emulsion of Sargassum extract.
Embodiment 3
1, the cooling ultrasonic dissolution assisting of vitamin C that takes 15.0g Sargassum extract, 20.0g Ovum Gallus domesticus Flavus lecithin, 3.0g stigmasterol and 0.05g, adds 20.0g Macrogol 4000, is warming up to 55-60 ℃, and to be stirred well to be evenly oil phase, insulation 10min.
2, take 10.0g propylene glycol, be added in 32.0g pure water, be warming up to 55-60 ℃, stir as water, insulation 10min.
3, will in oil phase material suction water, carry out premix, adjust rotating speed 10000r/min high-speed homogenization 20min, at 55-60 ℃ of insulation 10min, make colostric fluid.
4, colostric fluid is moved on in high pressure homogenizer, under 120MPa pressure, carry out high pressure homogenize circular treatment.In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, must be coated the skin repair function nano liposome emulsion of Sargassum extract.
Embodiment 4
1, the cooling ultrasonic dissolution assisting of superoxide dismutase that takes 15.0g Sargassum extract, 20.0g hydrolecithin, 1.0g stigmasterol and 0.1g, adds 15.0g Macrogol 4000, is warming up to 55-60 ℃, and to be stirred well to be evenly oil phase, insulation 10min.
2, take 15.0g propylene glycol, be added in 33.9g pure water, be warming up to 55-60 ℃, stir as water, insulation 10min.
3, will in oil phase material suction water, carry out premix, adjust rotating speed 10000r/min high-speed homogenization 20min, at 55-60 ℃ of insulation 10min, make colostric fluid.
4, colostric fluid is moved on in high pressure homogenizer, under 120MPa pressure, carry out high pressure homogenize circular treatment.In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, must be coated the skin repair function nano liposome emulsion of Sargassum extract.
Embodiment 5
1, take the cooling ultrasonic dissolution assisting of vitamin C of 15.0g Sargassum extract, 20.0g Ovum Gallus domesticus Flavus lecithin, 2.0g cupreol and 0.1g, add 10.0g Fatty acid macrogolglycerides, be warming up to 55-60 ℃, and to be stirred well to be evenly oil phase, insulation 10min.
2, take 20.0g butanediol, be added in 32.9g pure water, be warming up to 55-60 ℃, stir as water, insulation 10min.
3, will in oil phase material suction water, carry out premix, adjust rotating speed 10000r/min high-speed homogenization 20min, at 55-60 ℃ of insulation 10min, make colostric fluid.
4, colostric fluid is moved on in high pressure homogenizer, under 120MPa pressure, carry out high pressure homogenize circular treatment.In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, must be coated the skin repair function nano liposome emulsion of Sargassum extract.
Utilize Zetasizer Nano S90 nano-particle size analysis instrument, embodiment 2 gained liposome emulsions are carried out to Preliminary detection.As shown in Figure 1, utilize liposome technology to wrap up Sargassum extract, obtain having the mean diameter of skin maintenance function nano liposome emulsion, can remain on below 100nm.
Utilize projection electron microscope (Transmission Electron Microscope, TEM), embodiment 2 gained liposome emulsions are carried out to morphology characterization.As shown in Figure 2, the liposome of the parcel Sargassum extract obtaining is uniform-spherical structure, and particle size distribution is comparatively even.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. a nano-lipid precursor emulsion, is characterized in that, parcel Sargassum extract in nanometer liposome.
2. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, contains: Sargassum extract 10～20wt%, lecithin 10～20wt%, proppant 1～3wt%, antioxidant 0.01～0.1wt%, solubilizing agent 10～20wt%, stabilizing agent 10～20wt%.
3. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, the particle diameter of described nanometer liposome is at 10nm～100nm.
4. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, described lecithin is selected from one or more in soybean lecithin, Ovum Gallus domesticus Flavus lecithin and hydrolecithin.
5. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, described proppant is selected from one or more in cholesterol, stigmasterol and cupreol.
6. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, described antioxidant is selected from one or more in vitamin E, vitamin C and superoxide dismutase.
7. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, described solubilizing agent is selected from one or more in Tween 80, Macrogol 4000 and Fatty acid macrogolglycerides.
8. a kind of nano-lipid precursor emulsion according to claim 1, is characterized in that, described stabilizing agent is one or more in glycerol, propylene glycol and butanediol.
9. the preparation method of the nano-lipid precursor emulsion under claim 1-8 any one claim, is characterized in that, comprises the following steps:
Step 1, takes formula ratio Sargassum extract, lecithin, proppant, solubilizing agent, antioxidant, mixes and be stirred well to even oil phase under 55～60 ℃ of temperature conditions, is incubated 6～12min;
Step 2, the stabilizing agent that takes formula ratio is dissolved in pure water, is warming up to 55～60 ℃, stirs as even water insulation 6～12min;
Step 3, carries out premix in water prepared by oil phase suction step 2 prepared by step 1, uses homogenizer high-speed homogenization 15～25min, at 55～60 ℃ of insulation 6～12min, makes colostric fluid;
Step 4, moves on to colostric fluid in high pressure homogenizer, carries out high pressure homogenize circular treatment under 80～120MPa pressure; In the time that emulsion is extremely transparent or semitransparent, take out, naturally cool to room temperature, obtain nano-lipid precursor emulsion.
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