CN105125419B - A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof - Google Patents

A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof Download PDF

Info

Publication number
CN105125419B
CN105125419B CN201510646507.9A CN201510646507A CN105125419B CN 105125419 B CN105125419 B CN 105125419B CN 201510646507 A CN201510646507 A CN 201510646507A CN 105125419 B CN105125419 B CN 105125419B
Authority
CN
China
Prior art keywords
liposome
vitamin
polyethyleneglycol modified
facial mask
parts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201510646507.9A
Other languages
Chinese (zh)
Other versions
CN105125419A (en
Inventor
曹晓东
张禹
黄岳山
吴焯然
赵欣
胡光豫
陈钰蓝
肖斯宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China University of Technology SCUT
Original Assignee
South China University of Technology SCUT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China University of Technology SCUT filed Critical South China University of Technology SCUT
Priority to CN201510646507.9A priority Critical patent/CN105125419B/en
Publication of CN105125419A publication Critical patent/CN105125419A/en
Application granted granted Critical
Publication of CN105125419B publication Critical patent/CN105125419B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
    • A61K8/86—Polyethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0212—Face masks
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A61K8/553—Phospholipids, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/63—Steroids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/41—Particular ingredients further characterized by their size
    • A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/41—Particular ingredients further characterized by their size
    • A61K2800/413—Nanosized, i.e. having sizes below 100 nm
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/57—Compounds covalently linked to a(n inert) carrier molecule, e.g. conjugates, pro-fragrances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Cosmetics (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention belongs to daily nursing product technical field, discloses a kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof.The facial mask is to form hypothallus on backing fabric with polyethyleneglycol modified vitamin E liposome, then adds moisturizer, and is fixed by pad fabric, clipped to obtain；The polyethyleneglycol modified vitamin E liposome is made of the component of following parts by weight：10 100 parts of lecithin, 10 100 parts of cholesterol, 10 150 parts of vitamin Es, 200 600 parts of cholic acid are received, 400 4000 parts of polyethylene glycol and 400 800 parts of freezing drying protective agents.Facial mask of the invention, as active ingredient, is conducive to vitamin E and is stably present in skin layer and efficiently discharges for human skin absorption, the skin-care effect that wrinkles is removed with good using polyethyleneglycol modified vitamin E liposome.

Description

A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof
Technical field
The invention belongs to daily nursing product technical field, and in particular to a kind of polyethyleneglycol modified vitamin E liposome Facial mask and preparation method thereof.
Background technology
With the quickening of Urbanization Construction, air pollution is increasingly severe, and life stress is also increasing, and people are daily The generation of oxygen radical and gather increasingly severe in life, cause skin ageing phenomenon to aggravate, the probability of skin disease occurs Also it is increasing.A good skin-protection product is developed, cost can be effectively reduced but also efficiently eliminate oxygen radical.
Vitamin E has good cosmetic result.First, the pendant double bonds of vitamin E can directly participate in the oxidation of sulfydryl Reduction process, suppresses the activity of TYR enzyme, prevents pigmentation.Vitamin E also has expansion peripheral vessel, and it is micro- to improve blood vessel Circulation, promotes the conveying of nutritional ingredient and is metabolized the effect of rubbish excretion in vivo.In addition, vitamin E can protect cell membrane to make Its from free radical oxidative damage, so as to help to repair and consolidate the natural protective barrier of skin, pin the water of skin Point, there is provided nourishing from inside to outside.
However, skin care item often have ignored these cheap chemical compositions such as vitamin E currently on the market, and go to pursue Some are added to be difficult to extract but effect and vitamin E material very nearly the same, such as SOD (superoxide dismutase).SOD is one Kind is present in the enzyme in people or other biological body, can repair damaged cell, scavenging activated oxygen, but its extraction is suitable Difficulty, costly, so the skin care item price general charged containing SOD is higher, and actually the content of SOD can not possibly be very high, because This its skin effect is also restricted；And vitamin E is a kind of chemical composition being widely present, antiageing effect is good, most important It is that extraction is easy, cost is cheap, is adapted to large-scale production and commercialization.Secondly, many skin care item are all pin at present To moisturizing, such as skin care item containing glycerine, containing hyaluronic acid, but they can not produce the invasion and attack of ultraviolet and be effectively protected.
In addition, still suffer from some problems in existing vitamin E product：Directly encapsulated using phosphatide vitamin E and other Skin care ingredient；The O/W micro emulsions formed with glycerine and surfactant, distilled water wrap up vitamin E；Particulate containing vitamin E Particle diameter is bigger.Relevant patent such as " skin care item of resisting age of skin " (CN1345225), " complex capsule of vitamin E " And " a kind of vitamin E cream and its preparation method " (CN101011326) (CN101040933),.The above two are intended to explanation and use Liposome encapsulation prepared by above-mentioned method is than relatively low, and stability is poor, is traditional method for preparing lipidosome.Stability Difference is that surface of liposome carries electric charge, and this feature easily causes liposome to be identified as external source by the macrophage in blood Material and fallen by phagocytosis；CN101011326 is intended to illustrate that particle size can influence liposome and be absorbed by the skin through skin, particle diameter It is smaller, more readily penetrate through skin, the effect of generation is also more obvious.
Therefore, develop that a envelop rate is high, the skin care item of the strong flexible lipidosome containing vitamin E of percutaneous permeation, no Skin care cost can be only reduced, also significantly improves skin effect, there is important economic benefit and social benefit.
The content of the invention
The shortcomings that in order to solve the above prior art and shortcoming, primary and foremost purpose of the invention are to provide a kind of poly- second The vitamin E liposome facial mask of glycol modification.
Another object of the present invention is to provide the preparation side of above-mentioned polyethyleneglycol modified vitamin E liposome facial mask Method.
The object of the invention is achieved through the following technical solutions：
A kind of polyethyleneglycol modified vitamin E liposome facial mask, the facial mask are with polyethyleneglycol modified dimension life Plain E liposomes form hypothallus on backing fabric, then add moisturizer, and are fixed by pad fabric, clipped to obtain；Institute The polyethyleneglycol modified vitamin E liposome stated is made of the component of following parts by weight：10-100 parts of lecithin, 10-100 parts Cholesterol, 10-150 part vitamin E, 200-600 parts of cholic acid are received, 400-4000 parts of polyethylene glycol and 400-800 parts freeze-drying Protective agent.
The preferred physiological saline of the moisturizer.
The backing fabric and pad fabric is preferably hydrophilic non-woven, silk fabric or gauze.
The lecithin is selected from soybean lecithin, hydrogenated soy phosphatidyl choline, egg yolk lecithin, two palmityl phosphatidyl courages At least one of alkali, two nutmeg phosphatidyl cholines, Dilauroyl Phosphatidylcholine and synthetic phospholipid.
In the freezing drying protective agent preferably glycerine, lactose, sucrose, mannitol, glucose and trehalose at least It is a kind of.
The particle size range of the polyethyleneglycol modified vitamin E liposome is 50-200nm, Zeta potential for -30~- 55mV, envelop rate 70%-90%.
The preparation method of above-mentioned polyethyleneglycol modified vitamin E liposome facial mask, including following preparation process：
(1) lecithin, cholesterol, vitamin E and sodium taurocholate are weighed to be placed in container, adds the mixing of chloroform and methanol Solvent, stirring and dissolving uniformly obtain mixed solution；
(2) mixed solution is placed in Rotary Evaporators, vacuum extracts solvent at a temperature of 40-80 DEG C, is adhered to In the liposome membrane of wall；
(3) polyethylene glycol PBS buffer solutions are dissolved, prepare the polyglycol solution that mass concentration is 1%-20%, It is then added in the Rotary Evaporators of step (2), rotates aquation under the conditions of being 40-80 DEG C in temperature, obtain liposome suspension Liquid；
(4) by the liposome turbid liquor ultrasonic disperse of step (3), it is the saturating of 8000-14000 to be subsequently placed in molecular cut off Dialyse in analysis bag, remove free vitamin E；
(5) toward freezing drying protective agent is added in the system of step (4), it is freeze-dried after pre-freeze, obtains polyethylene glycol and repair The vitamin E liposome powder of decorations；
(6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add Moisturizer is added to carry out moisturizing；
(7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cut To a kind of polyethyleneglycol modified vitamin E liposome facial mask.
Preferably, the mixed solvent of the chloroform described in step (1) and methanol refers to that the volume ratio of chloroform and methanol is 1:1 Mixed solvent.
Preferably, the pre-freeze described in step (5) refers to be more than when -50 to -100 DEG C of pre-freezes 12 are small.
The present invention preparation method and obtained product has the following advantages that and beneficial effect：
(1) facial mask of the invention is conducive to dimension life using polyethyleneglycol modified vitamin E liposome as active ingredient Plain E is stably present in skin layer and efficiently release is for human skin absorption, so as to reach good content effect；
(2) as obtained by the preparation method of the present invention vitamin E liposome particle diameter in 50-200nm, surface charge it is absolute Value reaches 30mV to 55mV, can ensure the vitamin E component of parcel and have more preferable stability；Meanwhile use is polyethyleneglycol modified Liposome, is greatly improved the stability of liposome, and then improves envelop rate, and gained facial mask using effect is more preferable.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
A kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask, including following preparation process：
(1) egg yolk lecithin 0.08g, cholesterol 0.08g, vitamin E 0.04g and sodium taurocholate 0.2g are weighed and is placed in taper In bottle, it is 1 to add 20ml volume ratios:1 chloroform and the mixed solvent of methanol, stirring and dissolving uniformly obtain mixed solution；
(2) mixed solution is placed in Rotary Evaporators, rotatory vacuum extracts solvent in 42 DEG C of water bath with thermostatic control, obtains It is attached to the liposome membrane of wall；
(3) the 0.4g polyethylene glycol phosphate buffer of pH7.4 is dissolved, prepares the polyethylene glycol that mass concentration is 1% Solution, is then added in the Rotary Evaporators of step (2), when rotation aquation 1 is small under 40 DEG C of water bath conditions, obtains orange fat Plastid suspension；
(4) by the liposome turbid liquor ultrasonic disperse 5min of step (3), it is subsequently placed in the dialysis that molecular cut off is 8000 Dialyse in bag, remove free vitamin E；
(5) toward addition 0.8g mannitol in the system of step (4), freeze drier is placed on when -50 DEG C of pre-freezes 16 are small Middle freeze-drying, obtains polyethyleneglycol modified vitamin E liposome powder；
(6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add Enter physiological saline and carry out moisturizing；
(7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cut To a kind of polyethyleneglycol modified vitamin E liposome facial mask.
The average grain diameter of the vitamin E liposome of the present embodiment is 150nm, and Zeta potential is -49.5mV.
The envelop rate detection of the vitamin E liposome of the present embodiment：The orange liposome turbid liquor of step (3) is taken, is used 0.22 μm of filter filters bulky grain, then using supercentrifuge, centrifuges 45 minutes, takes under the conditions of 15000rpm 1.5mL centrifuge tube clear liquids, are placed on the absorbance surveyed in cuvette at 291.0nm, while survey the full liquid absorbance of the proportioning again. The envelop rate that the vitamin E liposome of the present embodiment is calculated by absorbance is 79.82%.
Embodiment 2
A kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask, including following preparation process：
(1) egg yolk lecithin 0.08g, cholesterol 0.08g, vitamin E 0.04g and sodium taurocholate 0.3g are weighed and is placed in taper In bottle, it is 1 to add 20ml volume ratios:1 chloroform and the mixed solvent of methanol, stirring and dissolving uniformly obtain mixed solution；
(2) mixed solution is placed in Rotary Evaporators, rotatory vacuum extracts solvent in 42 DEG C of water bath with thermostatic control, obtains It is attached to the liposome membrane of wall；
(3) the 0.4g polyethylene glycol phosphate buffer of pH7.4 is dissolved, prepares the polyethylene glycol that mass concentration is 1% Solution, is then added in the Rotary Evaporators of step (2), when rotation aquation 1 is small under 40 DEG C of water bath conditions, obtains orange fat Plastid suspension；
(4) by the liposome turbid liquor ultrasonic disperse 5min of step (3), it is subsequently placed in the dialysis that molecular cut off is 8000 Dialyse in bag, remove free vitamin E；
(5) toward addition 0.4g mannitol in the system of step (4), freeze drier is placed on when -50 DEG C of pre-freezes 16 are small Middle freeze-drying, obtains polyethyleneglycol modified vitamin E liposome powder；
(6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add Enter physiological saline and carry out moisturizing；
(7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cut To a kind of polyethyleneglycol modified vitamin E liposome facial mask.
The average grain diameter of the vitamin E liposome of the present embodiment is 130nm, and Zeta potential is -45.2mV.
The envelop rate detection of the vitamin E liposome of the present embodiment：The orange liposome turbid liquor of step (3) is taken, is used 0.22 μm of filter filters bulky grain, then using supercentrifuge, centrifuges 45 minutes, takes under the conditions of 15000rpm 1.5mL centrifuge tube clear liquids, are placed on the absorbance surveyed in cuvette at 291.0nm, while survey the full liquid absorbance of the proportioning again. The envelop rate that the vitamin E liposome of the present embodiment is calculated by absorbance is 70.34%.
Embodiment 3
A kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask, including following preparation process：
(1) egg yolk lecithin 0.08g, cholesterol 0.08g, vitamin E 0.03g and sodium taurocholate 0.4g are weighed and is placed in taper In bottle, it is 1 to add 20ml volume ratios:1 chloroform and the mixed solvent of methanol, stirring and dissolving uniformly obtain mixed solution；
(2) mixed solution is placed in Rotary Evaporators, rotatory vacuum extracts solvent in 42 DEG C of water bath with thermostatic control, obtains It is attached to the liposome membrane of wall；
(3) the 0.4g polyethylene glycol phosphate buffer of pH7.4 is dissolved, prepares the polyethylene glycol that mass concentration is 1% Solution, is then added in the Rotary Evaporators of step (2), when rotation aquation 1 is small under 40 DEG C of water bath conditions, obtains orange fat Plastid suspension；
(4) by the liposome turbid liquor ultrasonic disperse 5min of step (3), it is subsequently placed in the dialysis that molecular cut off is 8000 Dialyse in bag, remove free vitamin E；
(5) toward addition 0.6g mannitol in the system of step (4), freeze drier is placed on when -50 DEG C of pre-freezes 16 are small Middle freeze-drying, obtains polyethyleneglycol modified vitamin E liposome powder；
(6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add Enter physiological saline and carry out moisturizing；
(7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cut To a kind of polyethyleneglycol modified vitamin E liposome facial mask.
The average grain diameter of the vitamin E liposome of the present embodiment is 120nm, and Zeta potential is -40.6mV.
The envelop rate detection of the vitamin E liposome of the present embodiment：The orange liposome turbid liquor of step (3) is taken, is used 0.22 μm of filter filters bulky grain, then using supercentrifuge, centrifuges 45 minutes, takes under the conditions of 15000rpm 1.5mL centrifuge tube clear liquids, are placed on the absorbance surveyed in cuvette at 291.0nm, while survey the full liquid absorbance of the proportioning again. The envelop rate that the vitamin E liposome of the present embodiment is calculated by absorbance is 78.72%.
Embodiment 4
A kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask, including following preparation process：
(1) egg yolk lecithin 0.10g, cholesterol 0.10g, vitamin E 0.15g and sodium taurocholate 0.6g are weighed and is placed in taper In bottle, it is 1 to add 20ml volume ratios:1 chloroform and the mixed solvent of methanol, stirring and dissolving uniformly obtain mixed solution；
(2) mixed solution is placed in Rotary Evaporators, rotatory vacuum extracts solvent in 42 DEG C of water bath with thermostatic control, obtains It is attached to the liposome membrane of wall；
(3) the 4g polyethylene glycol phosphate buffer of pH7.4 is dissolved, it is molten prepares the polyethylene glycol that mass concentration is 1% Liquid, is then added in the Rotary Evaporators of step (2), when rotation aquation 1 is small under 40 DEG C of water bath conditions, obtains orange lipid Body suspension；
(4) by the liposome turbid liquor ultrasonic disperse 5min of step (3), it is subsequently placed in the dialysis that molecular cut off is 8000 Dialyse in bag, remove free vitamin E；
(5) toward addition 0.8g mannitol in the system of step (4), freeze drier is placed on when -50 DEG C of pre-freezes 16 are small Middle freeze-drying, obtains polyethyleneglycol modified vitamin E liposome powder；
(6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add Enter physiological saline and carry out moisturizing；
(7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cut To a kind of polyethyleneglycol modified vitamin E liposome facial mask.
The average grain diameter of the vitamin E liposome of the present embodiment is 180nm, and Zeta potential is -33.1mV.The present embodiment The envelop rate detection of vitamin E liposome：The orange liposome turbid liquor of step (3) is taken, with 0.22 μm of filter by big Grain filtering, then using supercentrifuge, centrifuges 45 minutes under the conditions of 15000rpm, takes 1.5mL centrifuge tube clear liquids, be placed on ratio The absorbance at 291.0nm is surveyed in color ware, while surveys the full liquid absorbance of the proportioning again.The present embodiment is calculated by absorbance The envelop rate of vitamin E liposome is 80.15%.
The polyethyleneglycol modified vitamin E liposome facial mask using effect test of the present invention：
The polyethyleneglycol modified vitamin E liposome facial mask of Example 1 is tried out by 30 people, and effect is used in one month Fruit table is as follows.Detection mode visually observes for user, wherein, all subjects have no adverse reaction, and are indicated in addition to two people Moistening effect, at the same polyethyleneglycol modified vitamin E liposome facial mask to less than 30 years old in puberty or Oily There is acne print effect per capita, have to more than 40 years old a middle-aged person and substantially go wrinkle effect, except individual subject, for annual age subject There is certain whitening effect.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (7)

  1. A kind of 1. polyethyleneglycol modified vitamin E liposome facial mask, it is characterised in that：The facial mask is repaiied with polyethylene glycol The vitamin E liposome of decorations forms hypothallus on backing fabric, then adds physiological saline moisturizer, and consolidated by pad fabric It is fixed, it is clipped to obtain；
    The polyethyleneglycol modified vitamin E liposome is made of the component of following parts by weight：10-100 parts of lecithin, 10-100 parts of cholesterol, 10-150 parts of vitamin Es, 200-600 parts of cholic acid are received, 400-4000 parts of polyethylene glycol and 400-800 parts Freezing drying protective agent；The particle size range of the polyethyleneglycol modified vitamin E liposome is 50~200nm, Zeta potential For -30~-55mV, envelop rate is 70%~90%.
  2. A kind of 2. polyethyleneglycol modified vitamin E liposome facial mask according to claim 1, it is characterised in that：It is described Backing fabric and pad fabric be hydrophilic non-woven, silk fabric or gauze.
  3. A kind of 3. polyethyleneglycol modified vitamin E liposome facial mask according to claim 1, it is characterised in that：It is described Lecithin be selected from soybean lecithin, hydrogenated soy phosphatidyl choline, egg yolk lecithin, dipalmitoylphosphatidylcholine, two nutmegs At least one of phosphatidyl choline, Dilauroyl Phosphatidylcholine and synthetic phospholipid.
  4. A kind of 4. polyethyleneglycol modified vitamin E liposome facial mask according to claim 1, it is characterised in that：It is described Freezing drying protective agent refer at least one of glycerine, lactose, sucrose, mannitol, glucose and trehalose.
  5. 5. a kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask of Claims 1 to 4 any one of them, It is characterized by comprising following preparation process：
    (1) weigh lecithin, cholesterol, vitamin E and sodium taurocholate to be placed in container, the mixing for adding chloroform and methanol is molten Agent, stirring and dissolving uniformly obtain mixed solution；
    (2) mixed solution is placed in Rotary Evaporators, vacuum extracts solvent at a temperature of 40-80 DEG C, obtains being attached to device The liposome membrane of wall；
    (3) polyethylene glycol PBS buffer solutions are dissolved, prepares the polyglycol solution that mass concentration is 1%-20%, then It is added in the Rotary Evaporators of step (2), rotates aquation under the conditions of being 40-80 DEG C in temperature, obtain liposome turbid liquor；
    (4) by the liposome turbid liquor ultrasonic disperse of step (3), it is subsequently placed in the bag filter that molecular cut off is 8000-14000 Middle dialysis, removes free vitamin E；
    (5) toward freezing drying protective agent is added in the system of step (4), it is freeze-dried, obtains polyethyleneglycol modified after pre-freeze Vitamin E liposome powder；
    (6) the liposome powder that step (5) obtains uniformly is applied on backing fabric, forms one layer of hypothallus, and add guarantor Humectant carries out moisturizing；
    (7) the backing fabric containing liposome matrix layer that step (6) obtains is fixed with one layer of pad fabric, cutting obtains one The polyethyleneglycol modified vitamin E liposome facial mask of kind.
  6. 6. a kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask according to claim 5, its feature It is：The mixed solvent of chloroform and methanol described in step (1) refers to that the volume ratio of chloroform and methanol is 1:1 mixing is molten Agent.
  7. 7. a kind of preparation method of polyethyleneglycol modified vitamin E liposome facial mask according to claim 5, its feature It is：Pre-freeze described in step (5) refers to be more than when -50 to -100 DEG C of pre-freezes 12 are small.
CN201510646507.9A 2015-09-30 2015-09-30 A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof Active CN105125419B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510646507.9A CN105125419B (en) 2015-09-30 2015-09-30 A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510646507.9A CN105125419B (en) 2015-09-30 2015-09-30 A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof

Publications (2)

Publication Number Publication Date
CN105125419A CN105125419A (en) 2015-12-09
CN105125419B true CN105125419B (en) 2018-04-13

Family

ID=54711251

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510646507.9A Active CN105125419B (en) 2015-09-30 2015-09-30 A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof

Country Status (1)

Country Link
CN (1) CN105125419B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105943488A (en) * 2016-06-30 2016-09-21 安徽天萝生物科技有限公司 Loofah skincare mask and preparation method thereof
CN110859798A (en) * 2019-10-31 2020-03-06 广州浚远康生物科技有限公司 Anti-aging repairing mask liquid containing human umbilical cord mesenchymal stem cell secretion and preparation method thereof
CN112603850B (en) * 2021-02-04 2023-09-19 雅弗生物实验室有限公司(加拿大) Vitamin C permanent magnet whitening anti-aging membrane cloth and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101780041A (en) * 2010-03-23 2010-07-21 南昌大学 Polymer coated vitamin E liposome and preparation method thereof
CN102058510A (en) * 2010-12-28 2011-05-18 海南京润珍珠生物技术股份有限公司 Leave-on facial mask with added pearl hydrolysate liposome
CN103432010A (en) * 2013-09-23 2013-12-11 上海相宜本草化妆品股份有限公司 Procyanidine liposome as well as preparation method and application thereof
CN103932897A (en) * 2014-04-25 2014-07-23 北京博辉瑞进生物科技有限公司 Composition for face moistening and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101780041A (en) * 2010-03-23 2010-07-21 南昌大学 Polymer coated vitamin E liposome and preparation method thereof
CN102058510A (en) * 2010-12-28 2011-05-18 海南京润珍珠生物技术股份有限公司 Leave-on facial mask with added pearl hydrolysate liposome
CN103432010A (en) * 2013-09-23 2013-12-11 上海相宜本草化妆品股份有限公司 Procyanidine liposome as well as preparation method and application thereof
CN103932897A (en) * 2014-04-25 2014-07-23 北京博辉瑞进生物科技有限公司 Composition for face moistening and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
脂质体的透皮作用及影响因素;曾珠等;《中国化妆品》;20080131(第1期);说明书第5页实施例8 *
面膜配方技术和面膜布材质概述;刘丽仙等;《日用化学品科学》;20150630(第6期);第6-9、44页 *

Also Published As

Publication number Publication date
CN105125419A (en) 2015-12-09

Similar Documents

Publication Publication Date Title
CN105963153B (en) Active compound and cosmetics or skin care item comprising blue copper victory peptide
CN105055185B (en) A kind of polyethyleneglycol modified vitamin E liposome and preparation method and application
CN103619415B (en) Exocellular polysaccharide for treatment and/or the nursing of skin, mucous membrane, hair and/or nail
KR102053280B1 (en) Fermented emulsifier forming reverse micell and composition comprising thereof and preparing method for fermented emulsifier
CN108904302A (en) A kind of fullerene topical composition
CN104997652A (en) Crease resistance and moisture retention liposome, and preparation method and application thereof
WO2009106338A2 (en) Cosmetic of dermopharmaceutical composition of mixed micelles
CN103893122B (en) A kind of madecassoside liposome and preparation method and application
KR20150005928A (en) Exopolysaccharide for the treatment and/or care of the skin, mucous membranes and/or nails
KR20070081192A (en) Cosmetic compostion comprising liposome incorporating oryzanol, rice bran oil and phospholipid
CN103585096A (en) Essence with functions of activating and tightening skin and preparation method thereof
CN107252109A (en) Function nutrition hardening agent composition and preparation method thereof
CN105125419B (en) A kind of polyethyleneglycol modified vitamin E liposome facial mask and preparation method thereof
CN105451710A (en) Exopolysaccharide for the treatment and/or care of the skin, mucous membranes and/or nails
CN104958241A (en) Moisturizing traditional Chinese medicine mask product and preparation method thereof
CN107496186A (en) A kind of Kaempferol nano-composition and its preparation method and application
CN104523472B (en) A kind of bamboo-leaves flavones complex liposome, preparation method and applications
CN105796459A (en) Moisturizing gel containing hydrolyzed pearl powder flexible nano liposome
KR101840045B1 (en) Liposomes encapsulating an oxazolidin-2-one compound
CN102988257B (en) Liposome sun-screening skin care product
CN114767559B (en) Azelaic acid liposome whitening cream and preparation method thereof
CN107737097A (en) A kind of anti-aging skin care product of the cell extract containing chrysanthemum and preparation method thereof
CN104983590B (en) A kind of nano liposomes powder of Chitosan Coating and preparation method thereof
CN107648087B (en) Whitening antioxidant skin cream and preparation method thereof
CN105943480A (en) Freckle-removing moisturizing lotion

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant