CN103610617A - Day cream containing soybean peptide and preparation method of day cream - Google Patents
Day cream containing soybean peptide and preparation method of day cream Download PDFInfo
- Publication number
- CN103610617A CN103610617A CN201310673078.5A CN201310673078A CN103610617A CN 103610617 A CN103610617 A CN 103610617A CN 201310673078 A CN201310673078 A CN 201310673078A CN 103610617 A CN103610617 A CN 103610617A
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- soybean
- day cream
- enzymolysis
- soybean polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of cosmetics, particularly relates to day cream containing soybean peptide, and also relates to a preparation method of the day cream. The day cream containing the soybean peptide comprises the following components: soybean peptide, a sambucus extract, olive oil, a humectant, a penetrating agent, a corrosion remover, a stabilizer, distilled water or normal saline. The day cream has the beneficial effects that soy protein which is peculiar in smell and poor in water-solubility and is not easily absorbed by the skin is hydrolyzed into the soybean peptide, and then the soybean peptide is combined with other components, so as to prepare the cream. The defects of the soy protein and the soybean peptide are overcome; the prepared cream is free of peculiar smell, is easily absorbed by the skin, can promote cell growth, differentiation and proliferation, and can keep the elasticity and the property of the skin.
Description
Technical field
The invention belongs to cosmetics technical field, be specifically related to a kind of day cream containing soybean polypeptide, also relate to the preparation method of this day cream.
background technology
Soybean polypeptide is the abbreviation of peptidyl soybean protein hydrolyate, that soybean protein is through the effect of protease, the proteolysis obtaining through special handling again produces, the essential amino acids of soybean polypeptide forms with soybean protein just the same, rich content and balance, and polypeptide compound is easily digested, and there is the effect of the anti-balance the body physiological function of curing the disease, soybean polypeptide is to have potential a kind of functional food ingredient, has become the health food of 21 century.
Soybean peptide is to take soybean protein as raw material, through biotechnology, processes, and the particular matter of the molecule amount size obtaining between protein and aminoacid, soybean peptide is mixed and is formed by multiple peptide, has the physiological regulation function that protein and aminoacid do not have.
The feature of soybean peptide is that essential amino acids content is abundant, arranges in pairs or groups balanced, contains isoleucine, leucine, lysine, methionine, phenylalanine, threonine, valine, tryptophan, 9 kinds of essential amino acids of histidine;
Infiltration rate is fast, and absorbing power is strong, can quickly supplement energy, and absorbing power is amino acid whose 3 times, is called as super aminoacid, during absorption, without degraded, directly absorbs;
Another one particularly, has biological activity.
When oppressing poultry meat and soybean protein when adding thermosetting gel or flour formation dough, add the soybean polypeptide of a few percent, gel is softening, this is because soybean polypeptide has stronger moisture absorption and moisture-keeping function, moisture is kept in goods, can play softened food, adjust its hardness, improve mouthfeel and keep the effects such as moisture.Therefore soybean polypeptide can be used as the various cosmetics of moisture retention Additive Production, and for example, soybean peptide can fully be mixed and made into shampoo and hair conditioner with various conditioner raw materials.Owing to containing abundant essential amino acids in soybean polypeptide; can with hair in disulfide bond interact; thereby hair is had to persistence protective effect; can improve hair quality; contribute to the reparation of hair damages, for another example, by soybean peptide, can prepare skin protection cosmetics; than the collagen peptide of previous cosmetic field extensive use, silk peptide has better effect.
Soybean polypeptide is widely used in food, cosmetics.Existing mechanism is given birth in the blood fat reducing of soybean polypeptide, and in zoopery, arginic increase can affect the concentration in cholesterol, and its mechanism may be the absorption that has increased the excretion of bile acid and reduced cholesterol.
While taking in soybean protein, there is change in the insulin in blood and glucagon ratio, affected the synthetic of liver cholesterol.
Affect the activity of the synthetic and ldl receptor of body apolipoprotein.
Eat soybean protein, monocytic ldl receptor active is higher 8 times than matched group.Visible soybean polypeptide can reduce blood fat, and it has prevention of obesity, reduces the effect of cholesterol, blood pressure lowering, low antigen, increase bone density, prevention of osteoporosis antioxidation.
Because soybean polypeptide has good digesting and assimilating and processing characteristics, cause the concern of people to it, adopt different enzyme system or different separation methods, can obtain many soybean active polypeptide classes, now chain length and the composition of zymolysis technique scalable polypeptide.In addition, larger progress has also been got in its physiological function research.
A large amount of aminoacid that soybean polypeptide hydrolysis produces, can form under certain conditions compounded amino acid complex, this complex is conducive to promote the growth promoter of strand cell, skin to aging corrupted has repair, human body is suitably taken in soybean peptide, can keep skin smooth flexible one, avoid too early appearance wrinkle.
Soybean peptide is very favourable to beneficial bacteria of intestinal tract group's growth and breeding, it can profitable probliotics group provide complete nutritional support, taking polypeptide makes the increase of intestinal bifid flora higher 75 times than normal person, and these probiotic bacterias effectively decompose the cholesterol that is discharged to enteric cavity, thereby stoped large intestine heavily to absorb cholesterol, the cholesterol level of serum decreases.
Soybean peptide has a large amount of reports to the effect of body shaping blood fat reducing, and also starts to be widely used America and Europe and Japan.Along with the reinforcement day by day to the understanding of soybean peptide and application, as a kind of new, functional factor of health food safely and effectively, utilize the mechanism of soybean peptide body shaping blood fat reducing and effect will be applied widely.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides that a kind of irritated rate is low, the reservation of the larger limit of active component and be easy to stop absorbed day cream containing soybean polypeptide and preparation method thereof for people.
The present invention realizes by following technical scheme:
Containing a day cream for soybean polypeptide, comprise the component of following portions by weight:
Soybean polypeptide 10-25 part
Fols sambuci williamsii extract 1-6 part
Olive oil 1-2 part
Wetting agent 1-4 part
Penetrating agent 1-3 part
Antiseptic 0.05-0.1 part
Stabilizing agent 1-3 part
Distilled water or normal saline 20-60 part.
Preferably, soybean polypeptide day cream of the present invention comprises the component of following portions by weight:
Soybean polypeptide 12-22 part
Fols sambuci williamsii extract 2-5 part
Olive oil 1-2 part
Wetting agent 1-3 part
Penetrating agent 1-2 part
Antiseptic 0.05-0.1 part
Stabilizing agent 1-2 part
Distilled water or normal saline 20-60 part.
Preferably, soybean polypeptide day cream of the present invention comprises the component of following portions by weight:
15 parts of soybean polypeptide
4 parts of Fols sambuci williamsii extracts
2 parts, olive oil
1 part of wetting agent
1 part of penetrating agent
0.06 part of antiseptic
1 part of stabilizing agent
40 parts of distilled water or normal saline.
In the component of soybean polypeptide day cream bag of the present invention:
Wetting agent is glycerol, 1, at least one in 3-butanediol, hyaluronic acid, lactic acid, sodium lactate, pyrrolidone sodium carboxylate, propylene glycol, vaseline, Trivent OCG, glucosan glycosides, poly-glycosaminoglycan, silk polypeplide, hydrolytic collagen and lecithin;
Above-mentioned penetrating agent is one or both in azone and dextran sulfate;
Foregoing preservatives is at least one in methyl parahydroxybenzoate, propyl p-hydroxybenzoate, isopropyl alcohol and propylene glycol;
Aforementioned stable agent is at least one in Polyethylene Glycol, cetostearyl alcohol, HANSHENGJIAO, CSSO3, methylol aminopolysaccharide, sulphuric acid mannan, dextran sulfate, polyglycine, glutathion and half amide acid, glyceryl stearate+PEG-100 stearate and cetearyl alcohol acid alcohol+cetearyl alcohol acidic group glucoside.
Preferred, soybean polypeptide day cream of the present invention comprises the component of following portions by weight:
15 parts of soybean polypeptide
4 parts of Fols sambuci williamsii extracts
2 parts, olive oil
1 part of hyaluronic acid
1 part of dextran sulfate
0.1 part of isopropyl alcohol
1 part of glutathion
40 parts of distilled water.
The preparation method of the day cream containing soybean polypeptide of the present invention, comprises following step:
(1) pretreatment of soybean protein
Take soybean meal as raw material, ungrease treatment;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 10%-15%, papain enzyme concentration 1-3%, hydrolysis temperature 45-60 ℃, enzymolysis time 2-4h, pH value 6.5-8.0,80 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 8.0-11.0, add the basic protein enzymolysis 2-4h of 1%-3%, 45 ℃-60 ℃ of hydrolysis temperatures, 90 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3-5% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby;
(3) preparation of day cream
Prepare soybean polypeptide 10-25 part, Fols sambuci williamsii extract 1-6 part, olive oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.1 part, stabilizing agent 1-3 part, distilled water or normal saline 20-60 part;
Add container for stirring to heat to 70-90 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned is placed in to vacuum homogeneous emulsifying machine by wetting agent, penetrating agent, antiseptic, stabilizing agent and distilled water or normal saline, and the speed of controlling homogeneous emulsifying machine is 2500-2800 γ/min; Temperature is 60-80 ℃, and persistent period 30-45 minute treats that homogenizing is cooled to 35-40 ℃, adds Fols sambuci williamsii extract, olive oil and soybean polypeptide, and keeping temperature is 35 ℃, continues to stir 10-20 minute, and bottling, sterilizing, be finished product after cooling.
In step (1), the organic solvent of defat is any in normal hexane, absolute ether, ethanol, acetone, dichloromethane.
Above-mentioned degreasing method is:
Adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
Or adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min.
The preparation method of the day cream containing soybean polypeptide of the present invention, comprises following step:
(1) pretreatment of soybean protein
Take bean cake as raw material, adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
Or adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby.
(3) preparation of day cream
Get soybean polypeptide 10-25 part, Fols sambuci williamsii extract 1-6 part, olive oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.1 part, stabilizing agent 1-3 part, distilled water or normal saline 20-60 part;
Add container for stirring to heat to 80 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned is placed in to vacuum homogeneous emulsifying machine by wetting agent, penetrating agent, antiseptic, stabilizing agent and distilled water or normal saline, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add Fols sambuci williamsii extract, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
The container that holds day cream is glass container or rustless steel container, because contain the composition of quintessence oil in day cream, olive oil its active component in thermal environment plastics environment is easily lost, and therefore adopts glass container to hold.
Owing to containing a large amount of globulin in soybean protein, the poorly water-soluble of globulin, directly applied to cosmetics for example in the production of day cream, in globulin and cosmetics, other composition is as very difficult mixing such as wetting agent, stabilizing agents, even merge preferably with above-mentioned material, also be difficult for being absorbed by skin, its active component can not be utilized by skin, can not play the effect that improves the skin quality colour of skin.
In addition, because part population is irritated to the soybean protein of high protein, as directly soybean protein being applied in the production of day cream, its irritated factor is not still eliminated, and the composition that easily causes skin allergy containing in the product occurring with cosmetics form still can continue to make skin allergy;
Therefore, in order to address this problem, the present invention is micromolecular polypeptide by soybean protein hydrolysis, the cosmetics of producing with polypeptide form are day cream for example, its performance has obtained larger change, water solublity strengthens greatly, is easy to produce and be easy to the cosmetics that absorb for human body skin with other the conventional adjuvant of cosmetics;
Due to the micromolecule polypeptide after hydrolysis, be different from the high protein of original macromolecule in addition, take micromolecule polypeptide as the cosmetics that raw material prepares, eliminated irritated factor, use crowd no longer produces allergic phenomena.
The soybean protein polypeptide that hydrolysis obtains has promotion cell differentiation, promote epidermis healing, increase the synthetic and secretion of extracellular matrix, promote capillary of skin net and little vascularization, the anti-ageing various biological effect of waiting for a long time, in research in anti-aging cosmetics produces, over the years biologically active polypeptide is comprised to cell growth factor particularly payes attention to, this class material is very micro-at people's in-vivo content, but biological activity is high, in various kinds of cell physiological function and metabolic activity, play important physiological regulatory action, affect directly or indirectly the growth of cell, division, differentiation, propagation and migration.
In the present invention, adopt olive oil as raw material, other vegetable oil that is different from of olive oil is that the linoleic acid that contains in olive oil is less, and iodine number is lower, and the unsaturated bond containing is few, compares with other liquid fat, not oxidizable in air, is difficult for occurring corrupt.And in olive oil, be rich in Squalene and the essential fatty acid splendid with skin affinity, absorb rapidly, with soybean polypeptide synergism, can effectively keep skin elasticity and moist, contained abundant monounsaturated fatty acid and vitamin E, K, A, D etc. and phenols antioxidant in olive oil, can eliminate facial wrinkles, prevent skin aging, have the effect of good skin protection.
In Fols sambuci williamsii extract, contain coenzyme Q10, coenzyme Q10 can allow skin cell metabolism faster, rubbish toxin is got rid of faster, thereby make skin more healthy, coenzyme Q10 can also reduce the infringement of the materials such as free radical to skin on the other hand, but also there is the effect of Polyglucan, and the soybean protein of high protein content may cause allergic phenomena, even if be hydrolyzed to micromolecular polypeptide, do not get rid of and wherein include not hydrolysis or the not soybean protein of complete hydrolysis yet, Fols sambuci williamsii extract is combined with soybean protein polypeptide, effective Polyglucan symptom.
Beneficial effect of the present invention is, by the soybean protein hydrolysis that has abnormal flavour, poorly water-soluble, be not easy to absorb for skin, it is soybean polypeptide, again soybean polypeptide is mixed mutually with other component, be prepared into facial cream, overcome the defect that soybean protein and soybean polypeptide itself has, prepared the facial cream free from extraneous odour of gained, easily by skin, absorbed, energy Promote cell's growth, differentiation, propagation, elasticity and the skin quality of maintenance skin.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
(1) pretreatment of soybean material: take bean cake as raw material, adopt organic solvent degreasing, the condition of defat is, the mass ratio of raw material and organic solvent is: 1:5-1:10, skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby;
(3) preparation of day cream
Add container for stirring to heat to 80 ℃ of left and right 40 parts of 1 part of hydrolytic collagen, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, 0.1 part of stabilizing agent glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of 4 parts of Fols sambuci williamsii extracts, 2 parts, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Comparative example 1
(1) pretreatment of soybean material: take bean cake as raw material, adopt organic solvent degreasing, the condition of defat is, the mass ratio of raw material and organic solvent is: 1:5-1:10, skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) preparation of day cream
Add container for stirring to heat to 80 ℃ of left and right 40 parts of 1 part of hydrolytic collagen, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, 0.1 part of stabilizing agent glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water or normal saline are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of 6 parts, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
With the difference of embodiment 1 be, only by soybean protein pretreatment, after pulverizing again after pulverizing defat, as raw material, do not comprise that with other adjuvant olive oil combines, prepare facial cream.
Comparative example 2
(1) pretreatment of soybean material: take bean cake as raw material, adopt organic solvent degreasing, the condition of defat is, the mass ratio of raw material and organic solvent is: 1:5-1:10, skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
(2) enzymolysis is prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby.
(3) preparation of day cream
Add container for stirring to heat to 80 ℃ of left and right 40 parts of 1 part of hydrolytic collagen, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, 0.1 part of stabilizing agent glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 2 parts, olive oil, 15 parts of soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
, in comparative example 2, not add Fols sambuci williamsii extract with the difference of embodiment 1; The ratio of olive oil is increased to 4 weight portions, and all the other are identical;
Human body is used experimentation containing the improvement effect of soybean polypeptide day cream to cortex wrinkle
Study subject and use
60 of female volunteers, age 23-50 year, be divided at random three groups, 1 group of 20 people of embodiment, comparative example 1, 2 groups of each 20 people, duration of test, the inactive any external thing in test position and/or cosmetics, experimenter is respectively by given the test agent and comparative example 1, 2 samples are applied to respectively left and right cheek and canthus, after getting up early every day and evening sleep before each once, continuous 8 weeks, adopt silicone rubber film coating method to copy the wrinkle of skin at the tested cheek of experimenter and position, canthus, numbering, perusal, evaluation criteria is according to the rules improved and is marked wrinkle degree, scoring group is comprised of 2 people, adopt double-blind method.
Result is as shown in the table:
The user that wrinkle depth improvement number smooths for wrinkle before and after using;
Wrinkle depth improves %=(use before-wrinkle depth after using)/wrinkle depth before using;
The average effective integration that smooths is high shows that formula is obvious to wrinkle improvement effect, is less than 0 for invalid.
In embodiment 1, day cream has the fragrance of pleasant, and exquisiteness is applied to skin, and skin absorbs fast, keeps immediately humidity after can be observed surface skin moistening, and skin surface is without coating;
In comparative example 1, day cream has abnormal flavour, coarse, is applied to skin, and skin is not easy to absorb, and forms the obvious coating of one deck on surface;
In comparative example 2, day cream has lighter abnormal flavour, and exquisiteness is applied to skin, and skin absorbs fast, keeps immediately humidity after can be observed surface skin moistening, and skin surface is without coating.
From above phenomenon, can find out, olive oil is collaborative mutually with soybean polypeptide, and covered soybean protein has abnormal flavour residual in peptide more, replaces as allowing the joyful fragrance of people;
From above, can find out relatively, soybean protein polypeptide is day cream prepared by raw material, the good absorbing effect of skin, take effect obviously, effectively improved the wrinkle depth of skin of face, have potential value, soybean polypeptide can promote and accelerate Growth of Cells, propagation, synthetic protein or peptide material, aspect alleviation skin aging and skin trauma reparation, is playing an important role.
Embodiment 2
(1) pretreatment of soybean material: take bean cake as raw material, adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, the condition of extraction is: extraction temperature is 45 ℃, and pressure is 25Mpa, time 180min;
(2) enzymolysis is prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby.
(3) preparation of day cream
Add container for stirring to heat to 70-90 ℃ 40 parts of 1 part of glycerol, 1 part of azone, 0.1 part of propyl p-hydroxybenzoate, 0.5 part of Polyethylene Glycol, 0.5 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 12 parts of 4 parts of Fols sambuci williamsii extracts, 2 parts, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Embodiment 3
(1) pretreatment of soybean material: take bean cake as raw material, adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, the condition of extraction is: extraction temperature is 45 ℃, and pressure is 25Mpa, time 180min;
(2) enzymolysis is prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby.
(3) preparation of day cream
Add container for stirring to heat to 70-90 ℃ 40 parts of 1 part of glycerol, 1 part of azone, 0.1 part of propyl p-hydroxybenzoate, 0.5 part of Polyethylene Glycol, 0.5 part of glutathion, distilled water, be incubated standby;
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 8 parts of 2 parts of Fols sambuci williamsii extracts, 2 parts, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
In the present embodiment, adopt papain and these two kinds of enzyme enzymatic hydrolysis of soybean albumen of alkaline protease, the peptide of micromolecule amount (molecular weight is less than 1000Da) accounts for 35.6% of total peptide content;
If adopt the papain enzymolysis soybean protein of same amount, the peptide of micromolecule amount (molecular weight is less than 1000Da) accounts for 31.1% of total peptide content;
And in soybean protein polypeptide, the polypeptide that plays physiological activity is mainly micromolecule amount, particularly molecular weight is less than the polypeptide of 1500 Da, if the content of peptides of micromolecule amount is higher, the physiological active functions of soybean protein polypeptide is also stronger.
From above, can find out relatively, two kinds of enzyme effects in the present embodiment are better than only adopting the consumption of the papain of same amount.
Embodiment 4
(1) pretreatment of soybean material: take bean cake as raw material, adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, the condition of extraction is: extraction temperature is 45 ℃, and pressure is 25Mpa, time 180min;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby;
(3) preparation of day cream
Add container for stirring to heat to 80 ℃ of left and right 40 parts of 1 part of hydrolytic collagen, 1 part of dextran sulfate, 0.1 part of isopropyl alcohol, 1 part of glutathion, distilled water, 1 part of Fructus Citri Limoniae essence, be incubated standby;
Add Fructus Citri Limoniae essence, can cover the light abnormal flavour of day cream, make its abnormal smells from the patient change the taste that makes people joyful into.
Above-mentioned raw material and distilled water are placed in to vacuum homogeneous emulsifying machine, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add 15 parts of 2 parts of Fols sambuci williamsii extracts, 2 parts, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Embodiment 5
In step (3), wetting agent is 1 part of glycerol, 1 part of silk polypeplide;
Penetrating agent is 1 part of azone and dextran sulfate;
Antiseptic is 0.1 part of methyl parahydroxybenzoate;
Stabilizing agent is 2 parts of methylol aminopolysaccharides.
All the other are identical.
Embodiment 6
In step (3), wetting agent is 2 parts, lecithin;
Penetrating agent is 1 part of azone;
Antiseptic is 0.2 part of propyl p-hydroxybenzoate;
Stabilizing agent is 1 part of polyglycine, 1 part of half amide acid.
All the other are identical.
Embodiment 7
In step (3), wetting agent is 1 part of glucosan glycosides, 1 part of poly-glycosaminoglycan;
Penetrating agent is 2 parts of azones;
Antiseptic is 0.1 part of methyl parahydroxybenzoate;
Stabilizing agent is 1 part of poly-dextran sulfate, 1 part of polyglycine.
All the other are identical.
Claims (9)
1. containing a day cream for soybean polypeptide, comprise the component of following portions by weight:
Soybean polypeptide 10-25 part
Fols sambuci williamsii extract 1-6 part
Olive oil 1-2 part
Wetting agent 1-4 part
Penetrating agent 1-3 part
Antiseptic 0.05-0.1 part
Stabilizing agent 1-3 part
Distilled water or normal saline 20-60 part.
2. a kind of day cream containing soybean polypeptide as claimed in claim 1, is characterized in that, described day cream comprises the component of following portions by weight:
Soybean polypeptide 12-22 part
Fols sambuci williamsii extract 2-5 part
Olive oil 1-2 part
Wetting agent 1-3 part
Penetrating agent 1-2 part
Antiseptic 0.05-0.1 part
Stabilizing agent 1-2 part
Distilled water or normal saline 20-60 part.
3. a kind of day cream containing soybean polypeptide as claimed in claim 1, is characterized in that, described day cream comprises the component of following portions by weight:
15 parts of soybean polypeptide
4 parts of Fols sambuci williamsii extracts
2 parts, olive oil
1 part of wetting agent
1 part of penetrating agent
0.06 part of antiseptic
1 part of stabilizing agent
40 parts of distilled water or normal saline.
4. a kind of day cream containing soybean polypeptide as described in any one in claim 1-3, it is characterized in that, described wetting agent is glycerol, 1, at least one in 3-butanediol, hyaluronic acid, lactic acid, sodium lactate, pyrrolidone sodium carboxylate, propylene glycol, vaseline, Trivent OCG, glucosan glycosides, poly-glycosaminoglycan, silk polypeplide, hydrolytic collagen and lecithin;
Described penetrating agent is one or both in azone and dextran sulfate;
Described antiseptic is at least one in methyl parahydroxybenzoate, propyl p-hydroxybenzoate, isopropyl alcohol and propylene glycol;
Described stabilizing agent is at least one in Polyethylene Glycol, cetostearyl alcohol, HANSHENGJIAO, CSSO3, methylol aminopolysaccharide, sulphuric acid mannan, dextran sulfate, polyglycine, glutathion and half amide acid, glyceryl stearate+PEG-100 stearate and cetearyl alcohol acid alcohol+cetearyl alcohol acidic group glucoside.
5. a kind of day cream containing soybean polypeptide as claimed in claim 4, is characterized in that, described day cream comprises the component of following portions by weight:
15 parts of soybean polypeptide
4 parts of Fols sambuci williamsii extracts
2 parts, olive oil
1 part of hyaluronic acid
1 part of dextran sulfate
0.1 part of isopropyl alcohol
1 part of glutathion
40 parts of distilled water.
6. the preparation method of a kind of day cream containing soybean polypeptide as claimed in claim 1, comprises following step:
(1) pretreatment of soybean protein
Take soybean meal as raw material, ungrease treatment;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 10%-15%, papain enzyme concentration 1-3%, hydrolysis temperature 45-60 ℃, enzymolysis time 2-4h, pH value 6.5-8.0,80 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 8.0-11.0, add the basic protein enzymolysis 2-4h of 1%-3%, 45 ℃-60 ℃ of hydrolysis temperatures, 90 ℃ of-95 ℃ of enzyme denaturing 10min-15min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1500Da;
Gained polypeptide solution adds 3-5% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby;
(3) preparation of day cream
Prepare soybean polypeptide 10-25 part, Fols sambuci williamsii extract 1-6 part, olive oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.1 part, stabilizing agent 1-3 part, distilled water or normal saline 20-60 part;
Add container for stirring to heat to 70-90 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned is placed in to vacuum homogeneous emulsifying machine by wetting agent, penetrating agent, antiseptic, stabilizing agent and distilled water or normal saline, and the speed of controlling homogeneous emulsifying machine is 2500-2800 γ/min; Temperature is 60-80 ℃, and persistent period 30-45 minute treats that homogenizing is cooled to 35-40 ℃, adds Fols sambuci williamsii extract, olive oil and soybean polypeptide, and keeping temperature is 35 ℃, continues to stir 10-20 minute, and bottling, sterilizing, be finished product after cooling.
7. the preparation method of a kind of day cream containing soybean polypeptide as claimed in claim 1, is characterized in that, in described step (1), the organic solvent of defat is any in normal hexane, absolute ether, ethanol, acetone, dichloromethane.
8. the preparation method of a kind of day cream containing soybean polypeptide as claimed in claim 1, is characterized in that, described degreasing method is:
Adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
Or adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min.
9. the preparation method of a kind of day cream containing soybean polypeptide as described in any one in claim 6-8, comprises following step:
(1) pretreatment of soybean protein
Adopt organic solvent degreasing, the condition of defat is that the mass ratio of raw material and organic solvent is: 1:5-1:10, and skimming temp 35-40 ℃, processing time 1-3 hour, defat is complete by organic solvent volatilization in fume hood;
Or adopt supercritical carbon dioxide extraction method to Semen sojae atricolor ungrease treatment, and the condition of extraction is: extraction temperature is 45 ℃, pressure is 25Mpa, time 180min;
(2) two step enzymolysis are prepared soybean polypeptide
With pure water, adjust concentration of substrate 12%, papain enzyme concentration 2%, 50 ℃ of hydrolysis temperatures, enzymolysis time 3h, pH value 7.0, enzyme denaturing 12min at 90 ℃ after enzymolysis completes;
After first step enzymolysis completes, regulate enzymolysis solution pH to 9.0, add 2% basic protein enzymolysis 3h, 60 ℃ of hydrolysis temperatures, 95 ℃ of enzyme denaturing 12min after enzymolysis completes;
Material filtering after enzyme denaturing is removed insoluble matter, obtains faint yellow clear solution, and this polypeptide solution is crossed supermicro filtration membrane, and collecting molecular weight is the filtrate below 1000Da;
Gained polypeptide solution adds 3% activated carbon adsorption decolouring, after macroporous resin DA201-C desalting processing, and vacuum concentration, lyophilization obtains dried soybean polypeptide powder, standby;
(3) preparation of day cream
Get soybean polypeptide 10-25 part, Fols sambuci williamsii extract 1-6 part, olive oil 1-2 part, wetting agent 1-4 part, penetrating agent 1-3 part, antiseptic 0.05-0.1 part, stabilizing agent 1-3 part, distilled water or normal saline 20-60 part;
Add container for stirring to heat to 80 ℃ wetting agent, penetrating agent, antiseptic, stabilizing agent, be incubated standby;
Above-mentioned is placed in to vacuum homogeneous emulsifying machine by wetting agent, penetrating agent, antiseptic, stabilizing agent and distilled water or normal saline, and the speed of controlling homogeneous emulsifying machine is 2600 γ/min; Temperature is 70 ℃, 40 minutes persistent period, treat that homogenizing is cooled to 35 ℃, and add Fols sambuci williamsii extract, olive oil and soybean polypeptide, keeping temperature is 35 ℃, continues to stir 15 minutes, bottling, sterilizing, be finished product after cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310673078.5A CN103610617B (en) | 2013-12-12 | 2013-12-12 | Day cream containing soybean peptide and preparation method of day cream |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310673078.5A CN103610617B (en) | 2013-12-12 | 2013-12-12 | Day cream containing soybean peptide and preparation method of day cream |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103610617A true CN103610617A (en) | 2014-03-05 |
| CN103610617B CN103610617B (en) | 2015-06-10 |
Family
ID=50161354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310673078.5A Active CN103610617B (en) | 2013-12-12 | 2013-12-12 | Day cream containing soybean peptide and preparation method of day cream |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103610617B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168032A (en) * | 2015-10-29 | 2015-12-23 | 福建农林大学 | Pure natural composite hand-protection emulsion and preparation method thereof |
| CN106389209A (en) * | 2016-11-01 | 2017-02-15 | 广东顺德希安雅珀生物技术有限公司 | Anti-aging facial mask |
| CN107913195A (en) * | 2016-10-07 | 2018-04-17 | 申扬阳 | Multi-effect sunscreen defence day cream |
| CN108785203A (en) * | 2018-07-13 | 2018-11-13 | 哈尔滨伟平科技开发有限公司 | A kind of production method of lip honey |
| CN110897958A (en) * | 2019-12-18 | 2020-03-24 | 广州和佳润颜医药有限公司 | Anti-aging composition and application thereof |
| CN112746091A (en) * | 2020-12-30 | 2021-05-04 | 南京工业大学 | Preparation and application of soybean protein peptide capable of increasing yield of bacillus subtilis nattokinase |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1723876A (en) * | 2005-07-12 | 2006-01-25 | 沈阳何氏眼科医院 | Eye-cream for developing eye cytoactive |
-
2013
- 2013-12-12 CN CN201310673078.5A patent/CN103610617B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1723876A (en) * | 2005-07-12 | 2006-01-25 | 沈阳何氏眼科医院 | Eye-cream for developing eye cytoactive |
Non-Patent Citations (2)
| Title |
|---|
| 莱斯利•布伦涅思: "《功效植物完全指南》", 30 April 2004, 中国友谊出版公司 * |
| 陈靓等: ""大豆分离蛋白酶解产物功能特性的研究"", 《大豆科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168032A (en) * | 2015-10-29 | 2015-12-23 | 福建农林大学 | Pure natural composite hand-protection emulsion and preparation method thereof |
| CN107913195A (en) * | 2016-10-07 | 2018-04-17 | 申扬阳 | Multi-effect sunscreen defence day cream |
| CN106389209A (en) * | 2016-11-01 | 2017-02-15 | 广东顺德希安雅珀生物技术有限公司 | Anti-aging facial mask |
| CN108785203A (en) * | 2018-07-13 | 2018-11-13 | 哈尔滨伟平科技开发有限公司 | A kind of production method of lip honey |
| CN110897958A (en) * | 2019-12-18 | 2020-03-24 | 广州和佳润颜医药有限公司 | Anti-aging composition and application thereof |
| CN112746091A (en) * | 2020-12-30 | 2021-05-04 | 南京工业大学 | Preparation and application of soybean protein peptide capable of increasing yield of bacillus subtilis nattokinase |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103610617B (en) | 2015-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103610617B (en) | Day cream containing soybean peptide and preparation method of day cream | |
| CN109512691A (en) | A kind of indigo plant copper peptide freeze-dried powder preparation and preparation method thereof | |
| KR100862979B1 (en) | Composition of Collagen Peptides for Cosmetics and Method Thereof | |
| CN103565728B (en) | A kind of black Fructus Lycii whitening skin and preserving moisture toner/smoothing toner and preparation method thereof | |
| CN109170469A (en) | A kind of Isin glue collagen peptide solid beverage and preparation method thereof | |
| CN1896268B (en) | Extraction method of activated micromolecular sheept germine from sheep embryo and its use | |
| CN102178636A (en) | Marine biological function cosmetic for minimizing pores | |
| CN102198063B (en) | Fish skin collagen peptide and olive oil compound skin-care product and preparation method thereof | |
| CN104644528B (en) | A kind of preparation method and application of edible moisture-keeping nutrient ointment | |
| CN109248138A (en) | A kind of anti-wrinkle cosmetic composition and preparation method thereof | |
| CN105687081A (en) | Anti-aging and moisture preserving gel and method for preparing same | |
| CN108618987A (en) | A kind of external preparation of skin nursing and preparation method thereof | |
| CN104523547A (en) | Preparation process research of andrias davidianus blanchard facial mask and application method of andrias davidianus blanchard facial mask | |
| CN103622879B (en) | A kind of day cream containing Peanut Polypeptide and preparation method thereof | |
| CN109430650A (en) | Prepare the formula and production technology of collagen, hyaluronic acid beverage and nourishing liquid | |
| CN103432059A (en) | Loranthus kaempferi paste egg white mask | |
| CN105395450A (en) | Premna microphylla lactic acid face application agent | |
| CN103610616B (en) | Night cream containing soybean peptide and preparation method of night cream | |
| CN113273655A (en) | Compound peptide protein solid beverage | |
| CN103622878B (en) | A kind of late frost containing Peanut Polypeptide and preparation method thereof | |
| CN103599054A (en) | Mask containing peanut protein polypeptide and preparation method thereof | |
| CN111110833A (en) | A skin caring composition for oral administration and topical application, and its preparation method | |
| CN110771902A (en) | Composite polypeptide powder and application thereof | |
| JP6875701B2 (en) | How to make proteoglycan | |
| CN105380874A (en) | Preparation method of seaweed mask |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SUN XIAOCHEN Free format text: FORMER OWNER: QUAN WEIYU Effective date: 20150505 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Sun Xiaochen Inventor before: Quan Weiyu |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: QUAN WEIYU TO: SUN XIAOCHEN |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20150505 Address after: 250014 Lixia District People's Hospital, 73 Wenhua East Road, Lixia District, Shandong, Ji'nan, China Applicant after: Sun Xiaochen Address before: 250014 Ji'nan 2012 middle school attached to Shandong University, Lixia District, Shandong City Applicant before: Quan Weiyu |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |