DE102018219593B4 - Chinese medical cosmetic composition - Google Patents

Abstract

Cosmetic composition made by mixing the following raw materials by weight percentage: radix notoginseng extract 5-10%, aloe extract 0.1-1%, Flos-Lonicerae extract 5-10%, dandelion extract 5-20 %, Ascorbic acid 2-3%, olive oil 12-17%, isopropyl palmitate 4-6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerol 4-6%, methyl p-hydroxybenzoate 0.08-0.12 %, Ethanol 6-8% and supplemental deionized water, where the radix notoginseng extract is prepared by the following steps: heat refluxing radix notoginseng with 50 mass% ethanol as a solvent twice, each time with an ethanol / radix notoginseng- Weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the radix notoginseng extract, wherein the aloe extract is prepared by the following steps: heat refluxing aloe twice with 70 mass% ethanol as a solvent, each time at an ethanol / aloe weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the aloe extract; wherein the Flos-Lonicerae extract is prepared by the following steps: heat-refluxing Flos-Lonicerae twice by 50 mass- % Ethanol as solvent, each time with an ethanol / Flos-Lonicerae weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the Flos-Lonicerae extract; and wherein the dandelion extract is prepared by the following steps: heat refluxing the dandelion twice at 90% by mass Ethanol as the solvent and each time at an ethanol / dandelion weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the dandelion extract.

Description

TECHNICAL AREA

The present invention relates to the field of cosmetics and medicine, particularly to a cosmetic composition, preferably a medical cosmetic composition, and more preferably a Chinese medical cosmetic composition.

GENERAL STATE OF THE ART

Everyone wants to have healthy and fair skin.

To prevent pigmentation, the basic principle of skin lightening is embodied mainly in the following aspects: 1. Prevention of melanin production, where melanin production is prevented by preventing tyrosinase activity or influencing melanin-producing intermediates. 2. Reduction of melanin and prevention of photo-oxidation, whereby the melanin reduction is stimulated by keratinocytes in order to remove the melanin produced. 3. Reduction of pigmentation during skin metabolism, whereby the melanin is rapidly secreted from the skin by improving the skin's metabolism. 4. Preventing UV light from entering, reducing the excess melanin caused by UV rays by physically blocking the UV rays with a sunscreen product.

Current whitening products are mainly used to prevent the activity of tyrosinase. Although there are many health care cosmetics, their usage is unsatisfactory for various reasons and cannot satisfy practical use. Therefore, improvement and renewal for cosmetics are urgently needed.

The skin is a large organ in the human body that is exposed to the external environment, and the water in the cells and matrix of the skin is easily lost. There is no blood vessel in the epidermis of the skin and the only way to get water is through penetration into the dermis, so the epidermis is the part of the human body that is most easily exposed to dehydration. The loss of water will impair skin cell division and secretion ability, reduce pigment metabolism and diminish the moisturizing effect itself. Effective replenishment and maintenance of the water in the skin is an important aspect that should not be neglected in reducing pigmentation in the skin. Keeping the skin hydrated is therefore the basis for spot removal and lightening.

SUMMARY OF THE INVENTION

In order to overcome the deficiency in the prior art, in view of the above, it is an object of the present invention to provide a cosmetic composition, particularly a Chinese medical cosmetic composition, which can efficiently solve the problems of cosmetics used for skin spot removal, moisturizing and whitening can be used.

The technical solution of the present invention is as follows:

The cosmetic composition, preferably the Chinese medicinal cosmetic composition, is made by mixing the following raw materials by weight percent: Radix Notoginseng Extract 5-10%, Aloe Extract 0.1-1%, Flos Lonicerae Extract 5-10%, Dandelion extract 5-20%, ascorbic acid (vitamin C) 2-3%, olive oil 12-17%, isopropyl palmitate 4-6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerol 4-6%, methyl p-hydroxybenzoate 0.08-0.12%, ethanol 6-8% and supplemental deionized water (i.e. the deionized water is added until the total percentage is 100%),
in which
the radix notoginseng extract is prepared by the following steps: heat refluxing radix notoginseng with 50 mass% ethanol as a solvent twice, each time with an ethanol / radix notoginseng weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours, and vacuum drying, to obtain freeze-dried powder as the radix notoginseng extract;
wherein the aloe extract is prepared by the following steps: heat refluxing aloe with 70 mass% ethanol as a solvent twice, each time with an ethanol / aloe weight ratio of (6-8): 1 for 1 hour to add two ethanol extraction solutions receive; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the aloe extract;
wherein the Flos-Lonicerae extract is prepared by the following steps: heat refluxing Flos-Lonicerae twice with 50% by mass of ethanol as a solvent, each time with an ethanol / Flos-Lonicerae weight ratio of (6-8): 1 for 1 h to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the Flos-Lonicerae extract;
and wherein the dandelion extract is prepared by the following steps: heat refluxing the dandelion twice with 90 mass% ethanol as a solvent, each time at an ethanol / dandelion weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions ; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours, and vacuum drying to obtain freeze-dried powder as the dandelion extract.

The cosmetic composition of the present invention, particularly the Chinese medical cosmetic composition, has a scientific and understandable formula composition, has the effect of tonifying Qi and nourishing Yin, promoting blood circulation and removing blood stasis, moisturizing and lightening
and strengthening the body and sunscreen can effectively remove free radicals, prevent tyrosinase activity, improve skin metabolism and reduce skin damage from UV rays, and is natural and non-irritating, so that it has the effect of full skin lightening.

DETAILED DESCRIPTION OF THE EMBODIMENTS

Then, the specific embodiments of the present invention will be explained in combination with the examples.

The present invention can be implemented with the following examples:

example 1

In one embodiment of the present invention, the cosmetic composition, in particular the Chinese medical cosmetic composition, is produced by uniformly mixing the following raw materials by weight percent: Radix notoginseng extract 7-8%, aloe extract 0.4-0.6%, Flos -Lonicerae extract 7-8%, dandelion extract 10-15%, ascorbic acid 2.3-2.6%, olive oil 13-16%, isopropyl palmitate 4.5-5.5%, polyoxyethylene nonylphenol ether 0.45- 0.55%, glycerol 4.5-5.5%, methyl p-hydroxybenzoate 0.09-0.11%, ethanol 6.5-7.5% and supplemental deionized water.

Example 2

In one embodiment of the present invention, the cosmetic composition, in particular the Chinese medical cosmetic composition, is produced by uniformly mixing the following raw materials by weight percent: Radix notoginseng extract 5%, aloe extract 0.5%, Flos Lonicerae extract 5% , Dandelion extract 8%, ascorbic acid 2%, olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerol 5%, methyl p-hydroxybenzoate 0.1%, ethanol 7% and supplementary deionized water.

Example 3

In one embodiment of the present invention, the cosmetic composition, in particular the Chinese medical cosmetic composition, is produced by uniformly mixing the following raw materials by weight percent: Radix notoginseng extract 9%, aloe extract 0.7%, Flos Lonicerae extract 7% , Dandelion extract 15%, ascorbic acid 2%, olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerol 4%, methyl p-hydroxybenzoate 0.1%, ethanol 8% and supplementary deionized water.

Example 4

In one embodiment of the present invention, the cosmetic composition, in particular the Chinese medical cosmetic composition, is produced by uniformly mixing the following raw materials by weight percent: Radix notoginseng extract 10%, aloe extract 1%, Flos Lonicerae extract 10%, dandelion - Extract 18%, ascorbic acid (vitamin C) 2.5%, olive oil 16%, isopropyl palmitate 6%, polyoxyethylene nonylphenol ether 0.4%, glycerol 6%, methyl p-hydroxybenzoate 0.11%, ethanol 8% and additional deionized water.

The above Chinese medicinal ingredients can work together with other components to achieve the effect of synergistic interaction, they have the effect of tonifying Qi and nourishing Yin, promoting blood circulation and removing blood stasis, moisturizing and lightening the body and To strengthen sunscreen, they can effectively remove free radicals, prevent tyrosinase activity, improve skin metabolism and reduce skin damage from UV rays, and are natural and non-irritating, giving them the effect of full skin lightening. The Chinese medical cosmetic composition of the present invention was tested to obtain a satisfactory advantageous technical level, and the test data were as follows:

Prevention of melanophore division

The melanin synthesis function of mouse B 16 melanocytes is essentially the same as that of normal melanocytes of human skin. The melanocytes were inoculated into a 96-well plate at 1 × 10 4 cells / well (100 µL per well) and grown in a 5% CO2 incubator at 37 ° C for 2 days; and the original culture solution was withdrawn and a complete culture solution was added. The above four Chinese medicinal extracts to be tested were proportionally mixed and diluted to make 5 solutions with different final medicinal concentrations of 500 mg / L, 250 mg / L, 125 mg / L, 62.5 mg / L and 31.25 mg / L to get. For each final concentration of the solution, five parallel wells were filled; and no medicine was added to a control group, but an equal amount of the complete medium was added, all of the above obtained test samples were further cultivated in the incubator for 3 days. The culture supernatant was removed, 100 µL of 0.5 mg.L-1 MTT was added to each well and the culture continued for 4 h; and 150 µL of DMSO were then added to dissolve the resulting purple precipitate, and the absorbance (A) was measured with a microplate reader at a wavelength of 570 nm (/ 630 nm). The absorbance value of each well is divided by the average absorbance value of the normal wells, and then multiplied by 100% to obtain a survival rate of each well and an inhibition rate = 1 - survival rate; and from the obtained inhibition rate curve, a lethal concentration range and a mean lethal IC50 concentration of the test samples were calculated, which indicated the toxicity index of the Chinese medicinal whitening ingredients (see Table 1); Table 1 Inhibition rate of melanocytes Medicinal Concentration (mg / L) 500 250 125 62.5 31.25 control Inhibition Rate% 64.10 ± 8.32 ▲ 49.66 ± 7.02 ▲ 31.16: 1: 4.82 ▲ 17.54 ± 3.45 ▲ 11.91 ± 2.05 ▲ 4.13 ± 0.84 Note: compared to the control group: ▲ P <0.05

With the increase in the concentration of Chinese medicinal whitening ingredients, the rate of inhibition of melanocytes has increased accordingly, and the toxicity index is 250 mg / ml, that is, the concentration of the Chinese medicinal whitening ingredients in a whitening skin cream cannot exceed this concentration of 250 mg / ml.

Inhibition of tyrosinase activity

After the Chinese medicinal whitening ingredients (at the above concentrations) worked for 3 days, the supernatant was discarded and each well was washed twice with pH 6.8 PBS and 90 µL of PBS containing 1% (volume percent) Triton X was added -100 contained. Each well was subjected to ultrasonic crushing in an ice bath, then received 10uL of 1 x 10-2 mol / L L-dopa, and was then incubated at 37 ° C for 60 minutes; and a UV spectrophotometer was used for colorimetry at 490nm, while zeroing was carried out with a blind trough to measure the absorbance value of each trough (see Table 2). Tyrosinase activity inhibition rate = (1-average absorbance value of each concentration / average absorbance value of the control group) * 100% Table 2 Tyrosinase activity inhibition rate Medicine concentration (mg / L) 500 250 125 62.5 31.25 Inhibition Rate% 37.98 32.44 26.34 19.26 5.94

It can be seen from Table 2 that with the decrease in the concentration of the Chinese medicinal whitening ingredients, the inhibiting effect on the tyrosinase activity was also reduced, that is, with the increase in the concentration of the medicine, its inhibiting effect was also improved, thus the inhibiting effect on the tyrosinase activity was confirmed.

Sun protection effect of the lightening skin cream

Five rats (weighing 400g-450g) were tested. After each rat was anesthetized with diethyl ether, 3cm x 3cm hair at the symmetrical parts on two sides of the rat's back was carefully cut off, and then the rat was placed in a rat cage and allowed to roam freely under UV sterile lamps (25W per sterile lamp at a distance of 100cm), an appropriate amount of the whitening skin cream was evenly applied to the right skin of the rat back, and only a cream containing no Chinese medicinal ingredients was applied to the left skin of the rat back; each rat was irradiated under the UV sterile lamp every day for 8 hours (after 12 hours, each rat was re-anesthetized and the whitening skin cream was applied once); the rats were removed, continued to be fed for 7 days, and then sacrificed. The surface of the rat skin was gently wiped with a cotton ball, which was alternately impregnated with water and ethanol to remove the remaining cream, and the thickness of the rat skin was measured with a vernier scale. Table 3 Measurement of skin thickness on the left and right sides of the rat (Unit: um) number 1 2 3 4th 5 Left side 48.6 42.9 45.6 50.1 47.8 right side 41.9 38.2 44.0 40.1 39.7

The UV damage can increase the thickness of the epidermal layer cells, UV rays will not only damage the outermost skin cells, but also reach the dermal cells through the epidermis with the result that necrosis and metabolic disorders of a large number of dermal cells occur, and the impurities cannot be metabolized in the cells, so the cells lose elasticity, age prematurely and become sensitive, and then wrinkles appear and thickness increase. As can be seen from the data in Table 3, the skin on the right side is thinner than the skin on the left, indicating that the skin on the right side of the rat's back to which the whitening skin cream has been applied is to some extent was protected.

Whitening skin cream moisturizing test

An in vitro weighing method is used (referring to the weighing method compiled in “Skin Science and Cosmetic Efficacy Evaluation” by Liu Wei and Zhang Huailiang). 5g of whitening skin cream and 5g of matrix were evenly applied to two culture flask caps each, then the culture flask caps were placed for 8 hours, the water reduction amount thereof being obtained by weighing every 1 hour, and the water retention capacity was calculated as follows: residual water mass / sample mass. Table 4: Water retention test of the whitening skin cream within 8 hours Time interval (h) 1 2 3 4th 5 6th 7th 8th Water retention capacity 98.24 90.84 89.34 87.12 86.51 79.24 60.46 57.12

As can be seen from Table 4, the amount of residual water was gradually reduced over time. After 8 hours, the water retention can be maintained at more than half, which indicates that a good moisturizing effect has been achieved.

In summary, from the above tests, it can be clearly confirmed that the Chinese medical cosmetic composition of the present invention exhibits the effects of blemish removing, moisturizing and sun protection, and can be effectively used for human skin protection and sanitation.

Claims (5)

Cosmetic composition made by mixing the following raw materials by weight percentage: radix notoginseng extract 5-10%, aloe extract 0.1-1%, Flos-Lonicerae extract 5-10%, dandelion extract 5-20 %, Ascorbic acid 2-3%, olive oil 12-17%, isopropyl palmitate 4-6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerol 4-6%, methyl p-hydroxybenzoate 0.08-0.12 %, Ethanol 6-8% and complementary deionized water, in which the radix notoginseng extract is prepared by the following steps: heat refluxing radix notoginseng with 50 mass% ethanol as a solvent twice, each time with an ethanol / radix notoginseng weight ratio of (6-8): 1 for 1 hour to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the radix notoginseng extract; wherein the aloe extract is prepared by the following steps: heat refluxing aloe with 70 mass% ethanol as a solvent twice, each time with an ethanol / aloe weight ratio of (6-8): 1 for 1 hour to add two ethanol extraction solutions receive; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the aloe extract; wherein the Flos-Lonicerae extract is prepared by the following steps: heat refluxing Flos-Lonicerae twice with 50% by mass of ethanol as a solvent, each time with an ethanol / Flos-Lonicerae weight ratio of (6-8): 1 for 1 h to obtain two ethanol extraction solutions; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the Flos-Lonicerae extract; and wherein the dandelion extract is prepared by the following steps: heat refluxing the dandelion twice with 90 mass% ethanol as a solvent and each time with an ethanol / dandelion weight ratio of (6-8): 1 for 1 hour to make two ethanol extraction solutions to obtain; Combining the two ethanol extraction solutions and recovering the ethanol from the result for concentration to obtain an extract having a specific gravity of 1.2-1.4; and freezing the extract at -80 ° C for 24 hours and vacuum drying to obtain freeze-dried powder as the dandelion extract. Cosmetic composition according to Claim 1 which is made by evenly mixing the following raw materials according to weight percent: Radix notoginseng extract 7-8%, aloe extract 0.4-0.6%, Flos-Lonicerae extract 7-8%, dandelion extract 10- 15%, ascorbic acid 2.3-2.6%, olive oil 13-16%, isopropyl palmitate 4.5-5.5%, polyoxyethylene nonylphenol ether 0.45-0.55%, glycerol 4.5-5.5% , Methyl p-hydroxybenzoate 0.09-0.11%, ethanol 6.5-7.5%, and supplemental deionized water. Cosmetic composition according to Claim 1 which is produced by evenly mixing the following raw materials according to weight percent: Radix-Notoginseng extract 5%, Aloe extract 0.5%, Flos-Lonicerae extract 5%, Dandelion extract 8%, ascorbic acid 2%, olive oil 15% , Isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerol 5%, methyl p-hydroxybenzoate 0.1%, ethanol 7% and supplementary deionized water. Cosmetic composition according to Claim 1 , which is produced by evenly mixing the following raw materials according to weight percent: Radix-Notoginseng extract 9%, Aloe extract 0.7%, Flos-Lonicerae extract 7%, Dandelion extract 15%, ascorbic acid 2%, olive oil 15% , Isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerol 4%, methyl p-hydroxybenzoate 0.1%, ethanol 8% and supplementary deionized water. Cosmetic composition according to Claim 1 , which is produced by evenly mixing the following raw materials according to weight percent: Radix notoginseng extract 10%, aloe extract 1%, Flos Lonicerae extract 10%, dandelion extract 18%, ascorbic acid 2.5%, olive oil 16% , Isopropyl palmitate 6%, polyoxyethylene nonylphenol ether 0.4%, glycerol 6%, methyl p-hydroxybenzoate 0.11%, ethanol 8% and supplementary deionized water.