TW201722445A - Glycerol production promoter derived from staphylococcus epidermidis, antimicrobial peptide production promoter derived from skin epidermal keratinocytes, and utilization thereof in external preparation for skin protection - Google Patents

Abstract

Provided are: a skin-protecting agent and an external preparation for skin protection, which not only synergistically work in combination with a plant polysaccharide but also has an effect of promoting the production of glycerol derived from Staphylococcus epidermidis, which is a skin resident bacterium useful on the skin, and the production of antimicrobial peptides derived from skin epidermal keratinocytes and exhibits excellent effect of improving the touch feeling when applied to the skin. More specifically, the present invention provides a skin protective agent and an external preparation for skin protection, which comprise a lactic acid bacterium belonging to the genus Enterococcus.

Description

An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, an accelerator for promoting the production of an antibacterial peptide derived from epidermal keratinocytes, and the use of an external preparation for skin protection

FIELD OF THE INVENTION The present invention relates to a production or a series of antibacterial peptides produced by promoting the production of glycerol derived from the resident bacteria of the skin, ie, Staphylococcus epidermidis as a beneficial bacteria, and from keratinocytes of the skin epidermis The function is to protect the skin from external stress such as dryness, or a drug for external use and a topical agent for skin protection.

BACKGROUND OF THE INVENTION Symbiotic microorganisms in human skin form a community in the form of a resident flora of the skin. As a resident bacteria of the skin, Staphylococcus epidermidis, Staphylococcus aureus, Corynebacterium, and the like survive, and the function of protecting the skin from external damage is maintained by the balance of the bacteria. However, it is known that environmental changes, rapid changes in temperature, dryness, strong detergents or medicines, or physical and mental factors such as trauma, mental stress, and general malaise caused by diseases can lead to a balanced collapse of these flora. This protection function is broken (Non-Patent Document 1).

For example, Staphylococcus epidermidis, which is known as a probiotic case of the skin, decomposes sebum to produce glycerin, and functions to prevent dry skin or to adjust the pH of the skin surface to inhibit the physical protection function such as the growth of the bacteria. Therefore, if the ratio of Staphylococcus epidermidis is reduced, such a protective function cannot be exerted, and it may become a cause of skin diseases. In order to prevent the skin disease, a moisturizing agent, an antibacterial agent, a bactericidal ingredient, and the like are gradually used in the external preparation for skin, but according to the pharmaceutical ingredients, the protective function of Staphylococcus epidermidis, which is an aerobic bacteria, is suppressed. .

In addition, Staphylococcus epidermidis also acts to stimulate skin epidermal keratinocytes, promote the production of antibacterial peptides derived from skin epidermal keratinocytes, and enhance the biological protective function of living organisms. As an antibacterial peptide produced by keratinocytes of the skin epidermis, human β-defensin-2 (hBD2: human β-defensin-2) and human β-defensin-3 (hBD3: human β-defensin- are known). 3), and it has been reported that these antibacterial peptides exhibit an antibacterial action against Staphylococcus aureus which is a harmful bacteria of the skin, and become a biological barrier to protect the skin from harmful bacteria (Non-Patent Document 2).

In the past, in order to promote the proliferation of S. epidermidis, sugars such as glucose oligosaccharides were used as an assimilation source (Patent Document 1), but since it is not only beneficial to Staphylococcus epidermidis, but also to other Staphylococcus aureus and other harmful bacteria. It shows assimilation, so there is also a situation in which the protective function of the skin is not achieved. Further, when the sugar is applied to the skin, it tends to be sticky, and other problems such as difficulty in formulating the touch are caused.

Staphylococcus aureus is known to be present in the skin of people with dry skin or chapped skin, or patients with atopic dermatitis. It is also known that toxins produced by S. aureus cause further deterioration of the skin condition. It is considered that in order to maintain the skin surface state health, it is important to effectively kill the S. aureus and maintain S. epidermidis having a skin protective function.

The effect of Staphylococcus epidermidis in the skin is similar to that of lactic acid bacteria or bifidus which are beneficial bacteria of the intestinal tract. It is known that these beneficial bacteria in the intestinal tract adjust the pH value in the intestinal tract to inhibit the proliferation of harmful bacteria such as Clostridium perfringens or Escherichia coli, and have a protective function of the intestinal tract.

In the intestinal tract, in order to improve the intestinal environment, ingestion of lactic acid bacteria or probiotic bacteria such as F. faecalis, in order to allow the beneficial bacteria to reach the intestine and be fixed on the intestine, it is necessary to select an acid-resistant strain or enteric solution. Type of encapsulation, etc. Further, in order to increase the beneficial bacteria in the intestine, a method of ingesting a food or drink containing various oligosaccharides which are selectively assimilated by dietary fiber or beneficial bacteria is also carried out. However, these methods also cause excessive intestinal fermentation or diarrhea due to differences in personal physique. As a method for solving such a problem, a method of heat-treating a dead cell using a lactic acid bacteria is provided (Patent Document 2).

Heat treatment of Enterococcus faecalis The inoculum is ingested by making a food or drink, which promotes the proliferation of F. faecalis in the intestinal tract, inhibits the proliferation of harmful bacteria, and improves the intestinal protective function. In addition, since it is a dead animal, it is easy to process as a food or drink, and it does not carry out unnecessary fermentation. Therefore, there are few cases in which the storage stability of foods and drinks is affected.

As the heat-treated dead cells of the lactic acid bacteria, microorganisms classified as Enterococcus faecalis can be suitably exemplified, and it is particularly preferable to use Enterococcus faecalis EC-12 strain (registered number FERM BP-10284), Enterococcus faecalis The 16SrDNA of the EC-12 strain (registered number FERM BP-10284) was registered as "AB15482" by the National Institute of Genetics of Japan. Prior Technical Literature Patent Literature

[Patent Document 1] Japanese Laid-Open Patent Publication No. 2005-002087 [Patent Document 2] Japanese Patent Laid-Open Publication No. 2004-051530 [Non-Patent Document]

[Non-Patent Document 1] Gallo, R. L., et al., Journal of Investigative Dermatology 131, P1974-1980 (2011) [Non-Patent Document 2] Kisich, K. O. et al., Journal of Investigative Dermatology 127: 2368-2380 (2007)

SUMMARY OF THE INVENTION Problems to be Solved by the Invention In order to maintain the health of the surface of the skin, it is indispensable to protect the skin from pressure or bacteria from the outside, such as drying. An object of the present invention is to provide one or a series of antibacterial peptides which are produced by promoting the production of glycerol derived from Staphylococcus epidermidis and against harmful bacteria such as Staphylococcus aureus derived from skin epidermal keratinocytes. It protects the skin from external stress such as dryness, or a drug for external use and a topical agent for skin protection. Means to solve the problem

The inventors of the present invention investigated various lactic acid bacteria and repeatedly conducted an effort to study, and as a result, it was found that the fungus of Enterococcus faecalis EC-12, which is a lactic acid-derived bacterium, has a glycerol derived from Staphylococcus epidermidis. The production and action of the antibacterial peptide derived from the epidermal keratinocytes of the skin. Moreover, when applied to the skin, it is very dry and very excellent in touch, and it imparts a feeling-improving effect to any of the aqueous, oily, emulsion, and powder-based external preparations.

That is, the present invention includes the following items. [1] An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, which comprises Enterococcus genus Lactococcus as an active ingredient. [2] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis according to Item 1, wherein the Enterococcus genus Enterococcus faecalis is Enterococcus faecalis. [3] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis according to the item 1 to 2, wherein the Enterococcus genus is Enterococcus faecalis EC-12 (registered number FERM BP-10284). [4] The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis according to any one of Items 1 to 3, wherein the Enterococcus genus Lactococcus is a dead cell. [5] The accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis according to any one of Items 1 to 4, wherein the Enterococcus genus Lactococcus is a heat-treated dead cell. [6] An accelerator for promoting the production of an antibacterial peptide derived from epidermal keratinocytes of the skin, which comprises Enterococcus genus Lactococcus as an active ingredient. [7] The accelerating agent for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the genus Enterococcus faecalis according to Item 6, wherein the Enterococcus faecalis is Enterococcus faecalis. [8] The agent for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the genus Escherichia coli according to the items 6 to 7, wherein the Enterococcus genus is Enterococcus faecalis EC-12 (registered number FERM BP-10284) . [9] The accelerating agent for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin according to any one of Items 6 to 8, wherein the Enterococcus genus Lactococcus is a dead cell. [10] The accelerating agent for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin according to the items 6 to 9, wherein the Enterococcus genus Lactococcus is a heat-treated dead cell. [11] The antibacterial peptide production promoter according to any one of claims 6 to 10, wherein the antibacterial peptide is human β-defensin-2 (hBD2: human β-defensin-2), human β-defensin-3 (hBD3: human β-defensin-3). [12] An external preparation for skin protection comprising the accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis according to items 1 to 11, and an accelerator for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin. Effect of invention

The agent for external use for skin or skin protection of the present invention can promote the production of glycerol derived from Staphylococcus epidermidis, and human β-defensin-2 which is an antibacterial peptide against Staphylococcus aureus derived from skin epidermal keratinocytes. , the production of human beta-defensin-3. In other words, by promoting the production of glycerin, it is possible to prevent dry skin, and it is also possible to promote the protective function of the skin such as regulating the pH of the skin surface and suppressing the growth of the bacteria. On the other hand, it is possible to promote the production of an antibacterial peptide against S. aureus derived from skin epidermal keratinocytes, and to protect the skin from harmful bacteria on the skin. Therefore, the health of the skin surface state can be maintained via the one or a series of promoting functions. Further, when applied to the skin, it is dry and very excellent in touch, and can impart a feeling-improving effect to any of the aqueous, oily, emulsion, or powder-based external preparations.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In the present invention, an accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, an accelerator for promoting the production of an antibacterial peptide derived from epidermal keratinocytes, or an external preparation for skin protection is contained in the present invention. Enterococcus genus Lactococcus is an active ingredient. The preferred method is to use the Enterococcus genus Lactococcus as the Enterococcus faecalis, especially the heat-treating dead cells of Enterococcus faecalis EC-12.

The agent for external use of the present invention or skin protection has the following characteristics. 1. Promote glycerol production from Staphylococcus epidermidis. 2. Further, by using in combination with a vegetable polysaccharide, synergistic promotion of glycerol production derived from Staphylococcus epidermidis is promoted. 3. Promote the production of antibacterial peptides against harmful bacteria such as Staphylococcus aureus derived from skin epidermal keratinocytes. 4. The function of protecting the skin from pressure or damage from the outside, such as drying, through one of the functions or a series of promoting functions. 5. When applied to the skin, it is excellent in dry touch, and it can impart a feeling-improving effect to any of the aqueous, oily, emulsion, and powder-based external preparations.

The method for culturing the bacterium of the genus Enterococci, which is a well-known method for culturing lactic acid bacteria, is not particularly limited, and the heat-treated dead cells can be produced by the following method. The cells can be recovered from the culture obtained by culturing the Enterococcus genus Lactococcus according to a conventional method by, for example, filtration, centrifugation, etc., washed with water, suspended in water or the like, heated, and then concentrated and dried as needed. Thereby, heat treatment of the dead cells is prepared. This can be carried out using a spray dryer, a freeze dryer or the like which is usually used. The enzyme treatment, the surfactant treatment, the grinding or the pulverization treatment may be carried out before or after the sterilization treatment by heating or the like, as the case may be, and the obtained by the treatment is also included in the dead bacteria of the present invention. Body or its treatment.

The water used for washing and suspending includes, in addition to pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, etc., including those in which various treatments are carried out. The treatment to be performed on water includes, for example, purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water usable as the extraction solvent in the present invention also includes purified water, heat-treated water, ion-exchanged water, physiological saline, various buffers, and the like.

The Enterococcus genus Lactococcus obtained by the above method can be used as an accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, and an antibacterial peptide production promoting against a bacterium such as Staphylococcus aureus derived from cutaneous keratinocytes. An external preparation for skin protection which protects the skin surface state by the one or a series of promoting functions.

Preferably, the accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, the antibacterial peptide production promoter derived from cutaneous keratinocytes of the skin of the present invention contains 5×10 of the above-mentioned heat-treated dead cells per gram. ^The suspension preparation is carried out in a manner of ¹¹ or more, particularly preferably 5 × 10 ¹² or more. Further, the external preparation for skin protection which is used daily is preferably prepared by blending one-third to the same amount per application.

(Form of the external preparation for skin protection) The external preparation for skin protection of the present invention may, for example, be an ampule, a capsule, a powder, a granule, a liquid, a gel, a bubble, an emulsion, a tablet, an aerosol, Sprays, etc. 1) pharmaceuticals, 2) quasi-drugs, 3) topical or systemic skin external preparations (such as lotions, lotions, creams, ointments, lotions, oils, etc.) Basic makeup materials such as masks, solid soaps, liquid soaps, hand lotions and other facial cleansers or cleansing lotions, massage agents, cleansing agents, hair removal agents, depilatory agents, shaving treatments, aftershave, shaving lotion, Shaving cream, foundation, lipstick, rouge, eye shadow, eyeliner, mascara and other make-up materials, perfumes, nails, nail polish, nail polish remover, dressing, plaster, patch, tablet, patch , aerosols, etc.), 4) medicinal or/and cosmetic preparations for scalp, hair (eg shampoos, cleansers, conditioners, pre-wash conditioners, styling solutions, hair dyes) , hair styling, hair growth, hair care / hair care, dressing, plaster, patch, Tablets, aerosols, etc.), 5) bathing agents for use in bath water, 6) anti-squeaking agents or deodorants, antiperspirants, hygiene products, sanitary napkins, wipes, and the like.

(Compositional component of the external preparation for skin protection) Further, such an external preparation for skin protection can be arbitrarily selected and used in the following exemplified components or additives, and the amount of the formulation is not particularly limited. It is generally considered to be about 0.0001 to 50%.

(1) various oils and fats such as avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, Atlantic chestnut oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid , Hawaiian stone fruit oil, safflower oil, shea oil, liquid avocado oil, soybean oil, camellia oil, corn oil, rapeseed oil, peach kernel oil, castor oil, cottonseed oil, peanut oil, turtle oil, oyster sauce, Egg butter, palm oil, palm kernel oil, wood wax, coconut oil, butter, lard, squalene, squalane, decane or a hydride of such oils (hydrogenated oil, etc.).

(2) Wax Beeswax, carnauba wax, cetyl wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice bran wax, and the like.

(3) Mineral oil Liquid paraffin, petroleum jelly, paraffin wax, ozokerite, ceresin, microcrystalline wax, and the like.

(4) fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linoleic acid, docosahexaenoic acid, eicosapentaenoic acid, Natural fatty acids such as 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isophthalic acid, caproic acid, 2-ethylbutyric acid, isovaleric acid, 2-methylpentanoic acid, 2 - Synthetic fatty acids such as ethylhexanoic acid and isovaleric acid.

(5) Alcoholic alcohol, isopropanol, lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytol, phenoxyethanol and other natural alcohols; 2-hexyl decyl alcohol, A synthetic alcohol such as stearyl alcohol or 2-octyldodecanol.

(6) Polyols such as ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol Monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butanediol, pentanediol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactose Alcohol, sorbitol, mannitol, lactitol, maltitol, and the like.

(7) Ester isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleic acid ester, decyl oleate, octyl myristate Dodecyl ester, hexyl decyl dimethyl octanoate, cetyl lactic acid, myristyl lactate, diethyl phthalate, dibutyl phthalate, acetylated lanolin, ethylene glycol Stearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound gum arabic, benzoin gum, dammar gum, guaiac resin, Irish moss, karaya gum, tragacanth, locust bean gum, Quercetin, agar, casein, lactose, fructose, sucrose or its ester, trehalose or its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, Addition of hydroxyalkyl (C ₂ -C ₄ ) chitin or chitosan, low molecular chitin or chitosan, chitosan salt, alkylene oxide (C ₂ -C ₄ ) such as ethylene oxide Sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl Cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, poly Vinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide or polypropylene oxide Polyalkylene oxide or a cross-linked polymer, carboxyvinyl polymer, polyethyleneimine and the like extend.

(10) Surfactant anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkylamine salt, alkyl quaternary Ammonium salt), amphoteric surfactant: carboxylic acid type amphoteric surfactant (amine type, betaine type), sulfate type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, Nonionic surfactant (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant) Other surfactants (natural surfactants, derivatives of protein hydrolysates, polymer surfactants, surfactants containing titanium-rhodium, fluorinated carbon-based surfactants).

(11) Various vitamins and vitamin A group: retinol, retinol (vitamin A1), dehydrogenation aldehyde (vitamin A2), carotene, lycopene (original vitamin A), vitamin B group: sulfuric acid Amine, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin , choline, inositol, vitamin C group: vitamin C acid or its derivatives, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydro tachysterol, vitamin E group : Vitamin E or its derivatives, ubiquinones, vitamin K group: phytomenadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), carbaryl Menadiol (vitamin K4), and essential fatty acids (vitamin F), carnitine, ferulic acid, γ-solanol, orotic acid, vitamin P (rutin, erionine, hesperidin) , vitamin U and so on.

(12) various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, aspartate Amine, glutamine, serine, cysteine, cystine, tyrosine, valine, hydroxyproline, aspartic acid, glutamic acid, hydroxy lysine, arginine, Ornithic acid, histidine or the like or an amino acid derivative such as a sulfate, a phosphate, a nitrate, a citrate or a pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These can be processed by a conventional method depending on the type and form of the product to be added (arbitrarily selected, for example, pulverized, pulverized, washed, hydrolyzed, fermented, refined) , pressing, extracting, distinguishing, filtering, drying, powdering, granulating, dissolving, sterilizing, pH adjustment, deodorization, decolorization, etc., are combined and selected from various materials.

Further, the solvent to be used for the extraction may be selected in consideration of the purpose of use, the type of the product to be provided, or the subsequent processing, etc., and it is usually preferred to use a solvent selected from the group consisting of water, methanol, ethanol, and propanol. a lower alcohol such as isopropanol, butanol or isobutanol or a polyhydric alcohol such as an aqueous lower alcohol, propylene glycol, 1,3-butylene glycol or glycerin or one of various organic solvents such as an aqueous polyol, acetone or ethyl acetate; Two or more kinds of mixed liquid. However, when it is not suitable to contain an organic solvent, it may be used only by using water, or ethanol which is easily removed after extraction, alone or in any mixture with water, or may be obtained by extracting and extracting.

In addition, when the additive derived from the plant or animal raw material is supplied to a systemic or topical external preparation or a cosmetic, in addition to the protection of the skin or hair and the moisturizing and tactile/texture improvement, Softness, relaxation of stimuli, relaxation of stress by aroma, cell activation (prevention of cell aging), inhibition of inflammation, improvement of skin texture/hair quality, prevention and improvement of skin splitting, hair growth, hair growth, prevention of hair loss In addition to the cosmetic effects such as the imparting of luster, the cleaning effect, the relaxation of fatigue, the promotion of blood flow, and the warm bath effect, effects such as flavor, deodorization, viscosity increase, antisepsis, and cushioning can also be expected.

Further, in addition to the above, various cosmetic and pharmaceutical effects of the raw material materials known so far can be expected, and by combining these, it is possible to obtain a product which achieves the improvement of the intended effect of the present invention and which is expected to have a multi-functional effect.

Specific examples of the plant (natural medicine) to be used as a raw material include almond (almond), indigo (blue leaf), breeze, coral, paulownia or paulownia, wood defense, qingtianma, artichoke, and red liter. Hemp, red currant "fruit", red clover, red grape, ink tree (hematoxylin), wild paulownia (wild paulownia), red sedge or yarrow or yarrow (grass root), red radix rehmannia or rehmannia (Rehmannia Glutinosa), Awei, East Asian T. chinensis, medlar (skin), Pouteria gardneriana, Maurui Palm, Tongcao (Mutong), Hemp Seed, Purple Flower, Morning Glory, Morning Glory, Hydrangea (Ziyanghua), Tomorrow Leaf, Red Bean ( Red Bean), Rohan cypress, Acerola, catechin, canna, anise, abiu, abiurana, rape, cork “fruit”, avocado, flax, sweet tea , esculentus, wilting (Yuzhu), Shanci Gu (Guangzigu), Amaranthus (Amaranthus) (苋, Yanlaihong, Taisui, Xianren Valley, Shiyan, Green Spike, Anti-branches, Hedgehog, Green Persimmon, Chiba Valley), Lily or Fritillaria, Alstromeria, and Betula (Alka) Nna tinctoria), Algarrobo, hollyhock, arnica, Alpinia katsumadai, aloe vera, real aloe vera, angelica, apricot (almond), benzoin, yellow sapote, gentian head, small E (Epimedium grandiflorum), rush grass, Polygonum cuspidatum (polygonum cuspidatum), yew, strawberry, fig "fruit, leaf", iris (Iris tectorum), ramie (Abutilon avicennae) (witch), deer Hoof grass, ginkgo "seed, leaf", Polygala tenuifolia, carob, scorpion (Draba nemorosa), Amaranthus blitum, rice "seed, seed coat", Aloehum graveolens "seed , Thymus quinquecostatus, ramie, ylang-ylang, Conandron ramondioides, Selaginella or rock pine, green beans, oolong tea, fennel, genipap (Huito, genipap), Jing Sanling (triangle) ), 莺藤 "fruit", fennel (Carum carvi), turmeric (turmeric), hydrang (duckweed), huaxinxin or liaxinxin or eusin (asaxin), malva (Malva sylvestris) , Althaea officinalis, Ponerorchis graminifolia, summer Grass, Aralia cordata (羌活, 唐独活), Uncaria Tomentosa, Uwa tea, plum (Ume "seed, flesh"), White-backed Quercus salicina, Bear grape leaf, Wenzhou mandarin orange (Chen's skin), Wenpu, Acacia, Acanthopanax, Green Persimmon, Echinacea (Echinacea), Cytisus scoparius, Flammulina velutipes, Senna obtusifolia or Ming, Hercampure, Sambucus nigra "fruit", eucalyptus, Pleurotus eryngii, 槐 (槐花, 槐花米), pea, scutellaria, Polygonatum falcatum, Queen Victoria Agave victoriae-reginae, berberine, cactus, Trichosanthes bracteata (including roots), Inula helenium, vine (anti-self), psyllium (Plantago), stagnation ( Rhaponticum uniflorum), Corylus heterophylla, Cereus giganteus, Atractylodes macrocephala, Lagerstroemia speciosa, Alnus sieboldiana, fruit Cereal grass (Eriocaulon b Uergerianum), Pouteria sapota, hawthorn, Lodiicea maldivica, barley, Oxalis tuberosa, Cnidium monnieri, okra "fruit", Eurasian flower (Inula) Britannica), Echinops latifolius (blue thorn), ginseng or bamboo ginseng, small forsythia or Hypericum perforatum (died cut grass), wild sesame (continued), Xanthium ( Xanthium), Juglans mandshurica, Hawthorn or Dioscorea (萆薢), Gastrodia elata, Lilium lancifolium or Lilium japonicum or Lilium brownii (Lily), Chondradia fauriei, Mangium flower, Ulmus laciniata, yellow dragon bud, Olluco, Nasturtium officinale (Cresson), Parsley (Petroselinum crispum), Psoralea corylifolia, Apium graveolens, olive "fruit, seed, leaf", oregano, orange "fruit, peel", Rehmannia glutinosa Libosch, cocoa "fruit, peel" Species , Persimmon (Persimmon "Leaf"), Uncaria (fishing hook), Glechoma hederacea (Lianqian), Persimmon, Hydrangea macrophylla, Cassia, Broussonetia papyrifera (楮 「 "fruit"), 莪 槲, eucalyptus (槲叶), cascarilla, Cascara sagrada, Xia Cao, Inula salicina, Haijinsha (金沙藤), Op (Chenopodium pallidicaule), carnation, valerian, birch, birch or Betula pendula (white birch), gerbera, pumpkin, jibe "seed", Zanthoxylum bungeanum (蜀 pepper), cattail (Pulver ), Chamomile or Roman chamomile, through grass, Myrciaria dubia, calla lily, Fumaria officinalis, Trichosanthes cucumeroides, Pinellia ternata, oats, melon Naina "Seed", Magnolia officinalis, Robinia pseudoacacia, Wolong, Papaya, Garcinia Cambogia, Agastache rugosa, Potentilla chinensis Ser. (White Grass), Water Bitter ( Veronica undulata), Yunzhi, Dianthus or Buckwheat (瞿 wheat) Artemisia apiacea, Heyuan Peng (Yin Chen), Ganzi, licorice, Cantala agave, candel tree, coltsfoot (Tussilago farfara), canna, olive, raspberry (raspberry, Dutch raspberry, White berry, red plum, raspberry, red raspberry), kiwi fruit "fruit, leaf", yellow squash (including root), platycodon (platform stem), chrysanthemum (chrysanthemum, chrysanthemum, Korean wild chrysanthemum), Gan Wild chrysanthemum, eucalyptus (salty), sheep's hoof (sheep hoof), medlar, ivy, ivy, Satureja, quinine, quinine, buckwheat (Chenopodium quinoa), jaundice (cork) ), pigeonpea, Gymnema sylvestre, Cereus peruvianus, cabbage, whole cabbage "immature fruit", caraway, cucumber, Tamarix (Xiheliu), saponaria (Quillaja saponaria) ), soap tree, stone pine (gold sore grass), kumquat "fruit", golden tortoise tree, camellia, Opuntia tuna, Piper betle, dragon tooth grass, golden orchid, silver Orchid, gold edge, Pouteria laurifolia, guava "fruit", gray and silver (Parthenium Argentatum), Quercus infectoria, Hawaiian drupe, Cephalanthera longifolia, medlar (scorpion, loquat leaf, ground bone), Asparagus, Ge (Pueraria), Eucalyptus, Gooseberry (Ribes uva-crispa) "fruit", Artemisia annua, Parashorea macrophylla, scorpion (Hawthorn), eucalyptus, Theobroma grandiflorum, 荜 茄, 笹 笹, verbena, Kushen, cranberry "fruit", chestnut" Seeds, fruits, endothelium, Curculigo Latifolia "fruit", grapefruit "fruit, leaf", buckthorn, iron holly (Ilex rotunda Thunb.) (salvation), sylvestris, clove (Ding Zixiang ), Luo Hancai (菥蓂, 菥蓂子), Nepeta (Schizonepeta), Celosia (Chicken), 昙花, Laurel, Bandit (Mountain Return), Gentian, Yak Seedling (Laoshan),枳椇 (枳椇子), the original species Dendrobium nobile, Citrus kinokuni (citrus, citrus, ponkan, citrus, citrus, grapefruit, orange peel), rose flowers, melissa ( Melissa officinalis), eucalyptus "fruit", black tea, palm grass ( Chuan Bone), Sakamoto, Sorghum, Galangal, Husong "fruit", Hu Huanglian, Scutellaria baicalensis Georgi, Bilberry, Coconut "fruit", Wusong, Raspberry cherries (Raspberry) , pepper, cosmos, Cuban balsam (Copaifera officinalis), coffee "seed, leaf", Magnolia or Magnolia (Xinyi), Burdock (Arctium sinensis), Female wilt (Clematis apiifolia), Flax, Scrophulariaceae, Schisandra, Schisandra Or schisandra, wheat, rice or rice bran "red peony, white peony", rice bran oil, Sudan cola fruit "seed", cola fruit "seed", boxwood leaves against vines (Coronilha), fenugreek "fruit", African anti-root, South American milk cane skin, kelp, medlar, Kang Fuli, sisal fibre, plucking orchid, bird's nest orchid (Hokkaido bird's nest orchid), Clematis chinensis, cherry blossom (Oshima cherry, mountain cherry, Dashan Sakura, Edo Sakura, Bean Sakura, Sakura Sakura, Sakura Yoshino Sakura, Kaolin Sakura, Kasama Sakura, Sakura Sakura, Sakura Sakura, Han Sakura "Leaf, Flower, Fruit, Bark (Sakura)", Cherry, Pomegranate, fine bamboo, long taro (Cephal) Anthera longibracteata), Chamei, Ou Zexie (Aesthesia), sweet potato, sugar cane, sugar beet, jujube (sautary jujube kernel), saffron (saffron), sapodilla, puffer fruit (Pouteria campechiana), cinnabar "fruit", soap Grass, American raspberry "fruit", cohosh, sand blue thorn, sage, large species of lobelia (mountain), water walnut (mountain), measuring scale, Peruvian dragon blood (croton) , three-leaf clover, panax notoginseng, hawthorn, mountain pepper, mountain bean root, avocado, avocado tree (Butyrospermum parkii) "fruit", shiitake mushroom (shii), asters, wings, digitalis, gentian, perilla or Green Perilla or Burmese Perilla or Single-sided Perilla (Perilla, Perilla), Rosewood, Eucalyptus, Magnolia officinalis, Admiralty (Forsythia), Shimeji (Brown-gray mushroom, smoke-colored pleated umbrella, Lotus leaf pleated umbrella, silver-white pleated umbrella, Hongxi mushroom, Zhenji pleated umbrella, maize, purple magnolia (Xinyi), spirea, potato, coral (salvia), jasmine (jasmine), along the order Grass (Maimendong), long kidney beans, starry sky, shrinking sand (Amomum, sand), Sichuan Valley, palm "fruit" Chunlan, Queen's Sunflower, Ginger, Changshan, Calamus (Calendula), Cardamom "fruit", green stalk "seed", white and white butterfly "seed", white clover (clover), chicken bone grass (Abrus fruticulosus ), Iris florentina, white flower vine "flower", white lupin, Nandina domestica, Cinchona succirubra, ruixiang (Ruixianghua, Shending), cinnamon (cinnamon), cinnamon ( Cinnamon), watermelon, honeysuckle (Lonicera), sorrel, Swim berry "fruit", star apple, stevia, strawberry "fruit", jingchaihu (Xu Changqing), thief (Qi Jing), purslane (purslane) ), plum "fruit", Jianlan (Xionglan), European red pine "fruit", European larch, ivy, Persian walnut, Western hazelnut, western dandelion, horse chestnut (conker), western pear Fruit", spirea, European elderberry (sambucus), European juniper (Juniper), European yam (western yarrow), yam, succulent, European mistletoe, peppermint or lemon balm, spicy Root, Shichangpu (Shijiegen), Shijie (Sakura 斛, original spring 斛, large Dendrobium, Okinawa Dendrobium, Dendrobium Dendrobium, Butterfly Dendrobium, Dendrobium Dendrobium, Dalbergia sinensis (dendrobium), lemon verbena (deodorant wood), mallow, broadleaf Meiyuanzhi, Meiyuanzhi, cress, celery, Chuanxiong , Andrographis paniculata, 旃 、, 刺 刺 cactus, senna "fruit, leaf", grape leaf clematis (Da 蓼), medicine, Chuan Pu, grass fruit, Lemaireocereus griseus, vegetarian heart plum, buckwheat "plant", potato Earthworms, broad beans, rhubarb, radish, soybeans, limes (orange peel, medlar), East China blue thorns (southern blue thorns), sputum (Eclipta prostrata), scorpion, golden dog hair fern (dog ridge) ), black sweet cherry "fruit", hollyhock, thyme (thyme), slender potato (萆薢), Liliu, tamarind "seed", onion, fragrant Xinyi (Xinyi), eucalyptus "fruit, leaf, root bark" , big leaf holly (one leaf tea, Kuding tea, bitter tea), dahlia (passiflora), variegated lupin, 痰 cut bean, single thorn cactus, salvia, dandelion or white flower dandelion or Mongolian dandelion, Danma, cherry "fruit", white stalk "fruit, root, bud", bamboo ginseng, chicory, bead grass (Phy Llanthus niruri), tulip, chocolate roh stay, artichoke, rhubarb (rhubarb), artichoke (artichoke), artichoke (芜荑), phoenix (Ai Ye), maitake (swine), grass worm, evening primrose , Neottia kiusiana, Agaricus bisporus (mushroom), Tutong grass, Camellia, Leigonggen, Xenopus laevis (lacquered grass), comfrey, red adzuki bean, Dendrobium chinensis, Polygonum, Apricot, goat milk (four leaves ), vine beans, lycopene, thorns, common walnuts, dew raspberry "fruit", stone cauliflower, sweet tea, Tiantai medicinal herbs (black medicinal), winter melon (winter melon), Huateng vine, chili (fan pepper), angelica , marigold, acacia, chuanchuan (chuanchuan), corn or corn mustard (maize), privet (femata), gentian or Korean gentian, wood thief, houttuynia, ipecac, dodder or Rootless, Eucommia bark, leaf, root, alfalfa (Qinpi), tomato, scutellaria, acacia "seed", eustoma, ocean sylvestris, Liaoyang, Mu Furong, Lingling Bean, Niger berry "fruit", Home yam or yam, butcher's broom, citron, leeks, rapeseed, bean, lime, jujube (jujube), Sakurako, Dioscorea, Park, Peanut (Rallow Peanut), Nantianzhu (South Tianzhu Fruit), Castor, Citron, Bitter Wood, Absinthe, Nutmeg, Gui or Cinnamon or Ceylon Cinnamon or Tiangui Gui (Cinnamon) or Gui Branch, Orchis joo-iokiana, leek (scorpion), elderberry "fruit, flower, stem, leaf", garlic (garlic), salted wood ( Gallic), onion, acacia or acacia or hell flower, wild donkey (大蓟), wild rose (camp), purple 葳 (霄霄花), pheasant crown, yarrow, earth angelica (formerly Hu), wild rose, green peach, pineapple "fruit", hibiscus (Zhu, chandelier Fusang, Luoshen Flower), slanted scorpion, lupin, Glycoxylon praealtum, scorpion fern (Bone scutellaria), white flower sphagnum, Orchis aristata, white sable, sour bilberry "fruit", sorghum, scorpion (scorpion), scorpion ), basil, lotus (lian, lotus), artichoke (parsley), rye, sweet potato, light bamboo or bitter bamboo (bamboo), mint, mint (mint), eight liters of beans, glutinous rice , Zhimu, banana, oregano, California poppy (蒺藜, hedgehog), vanilla bean, papaya, rat grass, Paharobobo, Bob "All grass, stems, leaves", red peppers, fine-brown fire-burning orchids, single-leaved vines or three-leaf vines (vines), fragrant aconite, medlar (scorpion), rose (rose), coral (side Windproof), witch hazel, palm, rose, balata, balata, edible toadstool (horse mushroom, ball-based mushroom), spider egg, green money willow (binzao), wall grass, spring cold orchid, Bitter gourd (vine lychee), spring 榆 (榆皮, 榆白皮, 榆 leaf), 尼古矛果 (Lonchocarpus nicou), spring gentian, fat sea, bread tree, shoot dry, vegetarian flower Codonopsis (codonopsis), Pecan , Bianhuahua (Rye and Garlic, Manzhu Shahua), Red Dahlia, Rough Blue Thorn, Rhombus (Rose), Pistachio, Sugar Radish, Dendrobium, Dye Wood, Daisy (Marguerite), Achyranthes, Orchis chidori , cypress, cypress leaf, ramie, sunflower, sweet pepper, sunflower, cattail, Agaricus blazei Murrill, sharp-skinned bird, allspice "fruit", white peony, white peony, leeks "fruit", chickpea , lentils, hollyhocks, pulsatilla, medlar, "fruit, leaves, stems", betel nut (big belly, betel nut), Pu'er tea (Pu'er tea) Phyllostachys pubescens (Nanteng), butterbur, winter dandelion (cold winter, coltsfoot), Zeeland (bluegrass), kidney bean (lentil), silk flower (t-sakura, silk flower), grape "fruit, peel" , seeds, leaves, beech, Cordyceps (Cornoptera), Brazilian licorice, Brazilian ginseng, Brazilian coconut, Brazilian ivory, blackcurrant "fruit", blackberry, Pradosian lactescens, plum "fruit", Furcellariaceae, blueberry (western orange ), prune, Floral blanca, psyllium, wendou (mung bean), hazelnut, loofah, phoenix, safflower, yellow agave, belladonna, raspberry "fruit", avocado, Peruvianberg, cherry unicorn, sedum , California blackberry "fruit", Kochia or broom grass (Kochia scoparia), venomous orchid, impatiens (phoenix, acute, stalk), horseberry, yolk, Japanese magnolia, wind, spinach, physalis , Japanese Magnolia (Pak), Papaya, Trichosanthes (Bitter Bitter), Lobular Insect, Atractylodes, Fine Leaf (Dingye Sanqi), Echinacea, Bodhi, Peony "Flower, Leaf, Stem , bark, peony, virgin, jojoba, borage, pollo (Boldo), Beet Raspberry "fruit", salt raw Cistanche (Cistanche, Dendrobium), Dendrobium Dendrobium (Huoshan Dendrobium), Yuye Tiannanxing (Tiannan Star), Maitake, Ephedra, Maca, Macadamia, Margaret Special flower (wood chamomile), amaranth (sea man grass), white mulberry (mulberry white bark, bark), Wudang magnolia, balata tree, Massaranduba rufula, Sideroxylon rugosum, Tropaeolum tuberosum ( Bulbous Golden Lotus), Wood Scorpio, Pine Ball, Pine "Leaf, Bark, Root", Pouteria salicifolia, Cloud 苓 (茯苓), Piper angustifolium, Marjoram (Oregano), Salix (Bitter ginseng), mulberry "fruit", eucalyptus, verbascum thapsus, mango, sage, chamomile (andean chamomile), walnut, citrus "fruit", ganoderma lucidum, citrus "fruit", Mishima Hu (Bupleurum), waterwheel front (grass), Lobelia chinensis, Japanese lyrics (Kalli), flat storage, sleeping vegetables, duck celery, green beauty, green mint, mimosa , 蘘和, mysterious fruit "fruit", myrrh, 梨梨勒, helichrysum, hibiscus, rough leaf Tree, Sapindus (Derived Skin), Muna (Minthostachys mollis), Lilac (Purple Root), Purple Beads or Japanese Purple Beads, Purple Corn, Old Jingteng (Kunming Spatholobus), Opuntia Megacantha, Motherwort, Basil, Melaleuca , melissa, yellow vanilla hibiscus, melon "fruit", Mongolian AI, Mengzong bamboo, wood unicorn, scented wood (Schinus molle), fine leaf holly, woody, palm leaf rhubarb, peach "leaf, seed, flower, fruit" , bean sprouts, Morillo black cherry "fruit", long jute jute (jute), purple potato, puzzle, ghost lamp (vector), immortal mint, holly, night fork gallnut (yellow) or cocoon Or Lushun Elm "fruit, peel, ear", Yang Bayberry, aniseed golden plate, mistletoe (willow parasitic), willow (willow, willow, weeping willow, glandular willow, pussy willow, cotton willow, willow, sharp purple Willow, Yunlongliu, Deep Mountain Willow, Coconut, Mountain Willow, Big Leaf Willow, Artemisia, Willow Vulpina, Liaoyang), Water Willow, Leaf, Stem, Blackberry, Purple Golden Cattle, Tianmingjing (Heqi, Tianmingjing), Shanglu, Alnus hirsuta, Yangmei (yangmeipi), mugwort, eucalyptus , Saxifrage, yucca or short-leaf yucca, pomelo "fruit", lily, white peony, AI (Ai Ye), Lei Wan, lime "fruit", rye, mangosteen "fruit", raspberry "leaf, fruit , Krameria triandra, Taro or Ashkelon (white), lavender, longan (longan meat), agave (green agave, agave), green tea, apple "fruit, Seeds, leaves, roots, gentian, sweet tea raspberry (rubus suavissimus), hard leaf blue thorn head (glazed jade, Xinjiang blue thorn head), litchi (Litchi nucleus), lettuce, redcurrant "fruit", red dragon Fruit, lemon "fruit", lemon grass, Admiral flower (Forsythia), Chinese milk vetch, wax plum, waxed coconut, reed root (scorpion), big bayberry "fruit", rosemary, rose hip (wild rose), hawthorn, Mi Menghua, cellar, etc.

As the seaweed, the following are exemplified: seaweed [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidea, Brassica (margin) Enteromorpha linza, Enteromorpha prolifera, Enteromorpha compressa, Enteromorpha intestinalis, Enteromorpha crinita, and sarcophagus (Dendrobium) Seaweed [Brown algae: kelp (Laminaria japonica), Laminaria ochotensis, Laminaria religiosa, Laminaria angustata), wakame, broadleaf wakame, green wakame, giant Algae (Macrocystis pyrifera, Macrocystis integrifolia, Macrocystis integrifolia), Sargassum fusiformis, Fucus algae, Dinoflagellates, Southern fanfish, P.australis var. cuneata, Cyanobacteria, large group of fan algae, Japanese group fan algae, small group fan algae, stalked fan algae], seaweed [red algae: camellia, stone cauliflower, flat stone cauliflower, Japanese cauliflower Chicken, Brassica, Hardy, Algae, Litchi, Litchi, Chick, Eucalyptus, Eucheuma, Eucheuma, Eucheuma, Carrageen, Giant Carrageen, Wrinkle Chondrus crispus, wedge-shaped carrageen, thorny carrageen, pinch carrageen, long carrageen, heterochromic carrageen, Japanese carrageen, pinch carrageen, cedar, Red soft thorn algae, small cedar algae, scalloped algae, algae with algae, algae, algae, red leaf algae, etc.

Further, other algae may be mentioned, for example, green algae (Chlamydomonas: Chlamydomonas, Polar algae, Dunaliella: Dunaliella, Prototheca: Prototheca, S. cerevisiae: Shigella , group algae: Volvox, four algae, four-spore, genus: spirulina, water cotton, bamboo genus, genus: silk algae, sarcophagus: Kong Shizhen , Net sarcophagus, Ashishi sarcophagus, brook genus: brook, genus of genus, genus Cladosporium: Japanese clostridium, C. serrata, clumps, genus, algae, cyanobacteria: large bubble method Cyanobacteria, cyanobacteria, and banana genus: banana, fern, pteridophyte, pteridophyte, serrata, pterygium, genus, genus: thorn , sub-cylindrical pine algae, coniferous pine algae, long pine algae, flat pine algae, cyanobacteria: Phytophthora, genus Chaetoceros: Helicobacter pylori, Phyllostachys hirsutum, Phyllostachys pubescens, new Phyllostachys genus, genus Chaetoceros, genus Cyanophyta, cyanobacteria: tennis cyanobacteria, reef genus: reef membrane, wide reef membrane, Gu's reef, thin kohlrabi, spirulina, Panaxella, Orange algae: orange algae, double star Genera, no algae are separated, etc.).

Cyanobacteria: Cryptophyta: Cyanophyta, Microcystis, Candida: Schizophyllum algae, Common Candida, Hairy Candida, Vibrio, Spirulina: Spirulina, Trameta, etc. ).

Brown algae (M. licheniformis: Phytophthora, water cloud genus: Indian water cloud, Polysaccharide: Polysaccharide, Brown plant: 褐-shaped brown-shelled algae, Phytophthora: Trichosanthes, sea wing Algae: filamentous seaweed, chlorella: cyanobacteria, chlorella, chlorella, and genus: genus algae, broadleaf genus, thick cyanobacteria: thick cyanobacteria, Thick-edged algae, Agaricus: Agaricus, Net-winged algae: Diptera, Hymenoptera: Diptera, Hymenoptera: Brown leaf algae, Cyanophyta: tree Cyanophyta, Cyanophyta, Cyanobacteria, Brachypodium, Brachypodium Genus: Ceratophyllum, Spirulina: Whip, Phytophthora: Phyllostachys pubescens: Phyllostachys pubescens, genus genus: genus, noodle genus: noodle algae, genus Chaetoceros: chlorella, Hydrilla verticillata Genus: Hydrilla verticillata, Phyllostachys genus: Dinoflagellate, Haiyun genus: Haiyun, Iron genus: Iron-nailed vegetables, leaf-shaped iron nails, and genus Pleurotus: Coronaria argentea, Trichophyton: Trichophyton : tangled seaweed algae, acid algae: lingual acid algae, acid algae, light brown acid algae, thick shore algae: smooth thick algae, genus genus: phylust, goose, genus: goose Leaf genus: Phyllostachys pubescens, Phytophthora: sphaerophyta, mesoporous genus: Japanese mesoporous algae, cyanobacteria: cyanobacteria, long cyanobacteria, Thousands of genus: Thousands of sacs, net Panax: Genus algae, Trichophyton sp.: Trichophyton sp., Neisseria genus: Species of Phyllostachys pubescens, Rock genus: Rock whiskers, Cyanophyta: Sphaerotheca fuliginea, Cercospora: 萱Algae, cyanobacteria: cyanobacteria, genus genus: spirulina, sphagnum: sphagnum, spirulina: spirulina, volcanic genus: wave algae, Japanese wave algae, kelp genus: leather kelp , Long-handled kelp, Ghost kelp, Shrimp kelp, Long kelp, Endo kelp, Laminaria cichorioides Miyabe, Lycium genus: Rhizoctonia solani, Quinum genus: Quinumbu, kelp genus: kelp, kelp, kelp, chrysanthemum Genus: Chrysanthemum kelp, Streptophyllum genus: Streptophyllum, Quercus genus: Heliconia genus, Rhizoctonia: Two-segment Algae, A. genus: two rounds of A. algae, winged algae: extended wing algae, thick-winged algae, carrageenan: white staghorn, leaf genus: Phytophthora, hornweed: trumpet algae, Cyanobacteria: Habitatella cerevisiae, Cyanobacteria, Cystophyllum turneri Yendo, Sargassum: Japanese sargasso, S. cerevisiae, Sargassum segii Yoshida, copper algae, linear Sargasso, striated sargasso, Sagitda , Sargassum nigrifolium Yendo, Sargasso, S. cerevisiae, Sargasso, Sargasso, Sargasso, Artemisia, S. cerevisiae, Spurs, Sargasso, S. , S. cerevisiae, genus genus: Phyllostachys praecox, macroalgae: macroalgae, genus Phytophthora: Phytophthora, genus genus: diatoms, etc.).

Red algae (Red genus: dark purple red cabbage, small red cabbage, laver: sweet seaweed, stripe laver, pseudo-line laver, long laver, Tian Zuo laver, multi-color laver, broad laver, red algae: Spirulina genus, Spirulina: Spirulina, Syzygium: Silk algae, Japanese powder algae, soft powder algae, hyssop: sea surface, multi-column surface, fork Branches of the sea surface, genus Phytophthora: Phytophthora, Phytophthora, and genus Pythium: Pythium, Fresh genus: Japanese fresh cyanobacteria, fresh genus: freshwater algae Chaetoceros: Fragile genus, Phytophthora: Phytophthora, Brassica genus, Ptilonia genus: Ptilonia okadae, Sphaerotheca: Sphaerotheca, Hymenoptera: Hook cypress Algae, yew-like hymen, genus of broccoli: small stone cauliflower, succulent cauliflower, big stone cauliflower, yarrow cauliflower, fascinating cauliflower, hydrangea (Hydrangea macrophylla), whisker, cauliflower, genus: Japanese soft Dichaeta, Dudresnaya minima, Leymus: Bright tube algae, Pichia: Pichia, Adhesive: thickened algae, single strip Algae, Genus: Irregular growth algae, Constantinea: Constantinea subulifera, new algae: Neodilsea yendoana, Neodilsea tenuipes, Genus : Fuyu soft-sea algae, Japanese soft-sea algae, red-tanned algae: North Shielded porphyra, Contarinia genus: Contarinia okamurae, Auricularia: Peyssonnelia caulifera Okamura, Auricularia: Japanese aphid, P. : Phyllostachys pubescens, coral genus: coral algae, genus genus: Asian cyanobacteria, Phyllostachys pubescens, Phyllostachys pubescens, Phyllostachys pubescens, Phyllostachys pubescens, Fusarium oxysporum, Phytophthora , elliptic algae, scorpion algae, scorpion scorpion algae, black spirulina, sea genus: genus genus, halymenia polydactyla, diatom spirulina, spirulina: spirulina, cypress: sea Phyllostachys pubescens: Shielded fruit algae, scutellaria, scutellaria, scutellaria, scutellaria, scutellaria, scutellaria, scorpion algae, horny algae, very high Algae, Phytophthora, and Cyanobacteria: Shishi , Phytophthora: Phytophthora, Schimmelmannia genus: Schimmelmannia plumosa, Hydrangea: small sea radish, sea radish, antler hawthorn, wall fruit algae: wall fruit algae, genus genus: jatropha, jasmine , Palmetto algae, Algae, Algae, Algae, C. mellifera, Callophyllis hayamensis, Prunus persicae, Taro, T. genus: M. argyi, Quercus: Trichophyton, Cinerella : Sclerotium, Kallymenia sagamiana, Kallymenia callophylloides, Spirulina: Japanese Spirulina, Serratia: Serratia, Tsengia lancifolia, Smooth Alga: Smooth Algae, Schizophyllum: Schizophyllum , Hydrilla verticillata: seaweed algae, genus genus: Phytophthora serrata, red leeks: sturdy red leeks, thin red leeks, cockatoo: felt-like cockscombs, snails: Tina algae, algae: clumps of algae, sea head red genus: sea head red, cartilage sea head red, sand genus: long branch sand vegetables, koji sand vegetables, mutated sand vegetables, Japanese sand vegetables, bunch of fruit Algae: Japanese fruit algae, Stemella: Stem algae, Auricularia The sea fungus and the genus Ganoderma lucidum: Zhenjiang 绳, 蓠江蓠, 脆江蓠, 粗江蓠, 内弯江蓠, 扁江蓠, 尾江蓠, 缢江蓠, 状状江蓠, 亚潮江蓠, Yamamoto scorpion, scorpion scorpion, phoenix edulis, sylvestris, sylvestris, scorpion scorpion, scorpion scorpion, scorpion scorpion, scorpion scorpion, scorpion scorpion, stellate genus : 缢江蓠, 突 藻 genus: Phyllostachys pubescens, with Cyclosporium: Acne with algae, Forked genus: Spirulina platensis, Quercus genus: Yigu algae, beautiful pseudo-algae, near Rongyi Algae, creek genus: brookside fern, genus genus: algae, Gigartina ochotensis, pacific algae, gingko genus: gingko genus, red genus: red genus, genus : Japanese genus Algae, cyanobacteria: Phytophthora sphaeroides, Phytophthora sphaeroides, genus Phytophthora: Golden algae, ginseng algae, genus genus: ganglia, arsenic, Brinell Low-stomach algae, grape algae: grape algae, cryptophyta: cryptophyta, palm-shaped algae: palm-shaped algae, intricate red algae, erythrophyllum, seaweed: bright seaweed, Cymbidium: Endocyst Algae, Phytophthora: Phyllostachys pubescens, Phyllostachys pubescens, Phyllostachys pubescens, genus genus: genus fluorescens, extended phylum, and genus: trigeminal genus, soft sauerkraut, Cone celery, Japanese celery, Bodeng celery, condensed genus: hooked coagulation, coagulation, and soft algae: beautiful soft algae, Renbao algae: Shishi Renbao, red leaf algae: Streptomyces argentea, Thousand Island Drosophila, Japanese new leaf algae, Dictyophora: Trichophyton, Polygonum: Japanese venom, New genus: M. new leaf, Myriogramme genus: Myriogramme polyneura, Hideophyllum yezoense, genus algae: genus Hooked algae, scalloped algae, algae, algae, Hymenena genus: Hymenena tenuis, red genus: crisp red nettle, amaranth: none Amaranth, Trichoderma: Sphaerotheca fuliginea, Heterologous genus: Japanese spirulina, beautiful spirulina, Pseudomonas genus: Pleurotus ostreatus, Polygonum: Inner tube multi-algae, Bacillus subtilis, Amaranthus: Amaranth, Cartilage: Dendritic algae, Caragana, Phyllostachys, entanglement Cartilage algae, spheroidal cartilage, cartilage, cartilage, scutellaria, scutellaria, scutellaria, scutellaria, scutellaria, scutellaria, pterygium: pteropod , pteridophyte, Duchesta: genus Mossy, Dipterophyta, Lepidoptera, C. cerevisiae: C. elegans, C. elegans, Convolvulus: Black whirl Flower algae, Japanese spirulina, Amansia mitsuii Segawa, first genus: genus of the village, genus, genus: genus, genus, genus: genus, genus Phytophthora sinensis, Odonna: Odonthalia corymbifera, Phytophthora: Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus ostreatus, Hot spring red algae: hot spring red algae, pseudo-Hyperthes: quasi-sea surface, Polygonum, Chlorella: Porphyridium, Red Solanum: Red Solanum, etc.).

Axillary algae (Cyanophyta, Litsea, Pseudomonas: blunt-spotted Pseudomonas, Trichophyton, Liga: soft-curved algae, pointed-headed algae, avian algae, etc.), yellow algae (Singapore genus: Roche diatom, etc.) and so on.

Further, examples of the material derived from the animal material include: cockscomb extract, bovine or pig, human placenta extract, bovine or swine stomach, duodenum, intestine, spleen extract or its decomposition product, cow or pig. Extract of brain tissue, collagen hydrolysate (acid, alkali, enzyme, etc.) of bovine, pig or fish, or collagen derivative such as water-soluble collagen or deuterated collagen, elastin or elastin of bovine or pig Hydrolysate (acid, alkali, enzyme, etc.) or water-soluble elastin derivative, keratin and its decomposition products or derivatives thereof, silk protein and its decomposition products or derivatives thereof, pig or bovine blood globulin Decomposition (bead peptide powder), bovine or porcine hemoglobin breakdown (chlorine hemoglobin, hemoglobin, prohemoglobin, native heme, heme iron, etc.), milk, casein and its decomposition products or derivatives thereof , skim milk powder and its decomposition products or derivatives thereof, lactoferrin or its decomposition products, egg components, fish decomposition products, nucleic acid related substances (ribonucleic acid, deoxyribonucleic acid) and the like.

Further, as a material derived from a plant or animal material, any part derived from a genetic recombinant or a cell fusion, a cell, a tissue, an organ, a metabolite, or the like can be used. Further, for example, an undifferentiated cell population or a cell population in the middle of differentiation obtained by cell culture of an arbitrary site, a cell, a tissue, an organ or the like can be used.

(14) Seawater such as seawater deep water, such as seawater salt, seawater dry matter, inorganic salts (sodium chloride, magnesium chloride, potassium chloride, etc.) obtained from the Dead Sea, the Atlantic Ocean or the Pacific Ocean, sea mud or mud ( Class of fango), such as Italian mud, German mud, Eifel mud, Freiburg mud, etc. (including ingredients: cerium oxide, titanium dioxide, aluminum oxide, iron oxide, Manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, barium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), Shengde stone, and the like.

(15) Microbial culture metabolites Yeast metabolites, yeast extracts, bacterial metabolites, bacterial extracts, mold or actinomycetes metabolites, mold or actinomycete extracts, natto metabolites, natto extracts, rice bran Extract, rice bran (red peony, white peony) fermentation extract, euglena extract or its decomposition product or such water-soluble derivatives, trehalose or its derivatives, lactose or skim milk powder, lactic acid mash, beans A lactic acid bacteria mash of a plant, a lactic acid bacterium of a coconut plant, and the like.

(16) α-hydroxy acid glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, and the like.

(17) Inorganic pigments phthalic anhydride, magnesium citrate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconia, magnesia, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron oxide yellow, Iron, iron oxide black, ultramarine blue, chromium oxide, chromium hydroxide, carbon black, heteropolar ore.

(18) Ultraviolet absorption/blocking agent benzophenone derivative (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2 -Sodium hydroxy-4-methoxybenzophenone-5-sulfonate, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonate, 2,4-di Hydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid derivative (p-aminobenzoic acid, ethyl p-aminobenzoate, p-aminobenzoic acid, p-dimethylamino) Amyl benzoate, octyl p-dimethylaminobenzoate, etc.), methoxycinnamic acid derivative (ethyl p-methoxycinnamate, isopropyl p-methoxycinnamate, octyl p-methoxycinnamate) Ester, 2-ethoxyethyl p-methoxycinnamate, sodium p-methoxycinnamate, potassium p-methoxycinnamate, di-p-methoxycinnamic acid mono-2-ethylhexanoate, etc.) , salicylic acid derivatives (octyl salicylate, phenyl salicylate, salicylic acid high * 艸 * * ester, dipropylene glycol salicylate, ethylene glycol salicylate, salicyl myristate, Methyl salicylate, etc., an ortho-aminobenzoic acid derivative (methyl ortho-benzoate, etc.), urine Acid derivatives (urinary acid, uric acid ethyl ester, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, * 咢 咢 * alkane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, horse chestnut bark Glycosides, benzyl cinnamate, chinox, oxybenzone, benzophenone, octyl ketone, sulfonone, phenylhydrazone, arbutin, guaiac, shikonin, jaundice Baicalin, baicalein, berberine, butyl methoxybenzhydryl methane, Escalol, zinc oxide, talc, kaolin, and the like.

(19) a whitening agent-amino benzoic acid derivative, a salicylic acid derivative, an ortho-aminobenzoic acid derivative, a coumarin derivative, an amino acid compound, a benzotriazole derivative, a tetrazole derivative, Imidazoline derivative, pyrimidine derivative, bis-quinone* alkane derivative, camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin C Or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or a derivative thereof, kojic acid or a derivative thereof, oxybenzone, benzophenone, arbutin, guaiac, Zygoxin, baicalin, baicalein, berberine, placental extract, turmeric, ruthenium, etc.

(20) tyrosinase activity inhibitor vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), Kojic acid or a derivative thereof, vitamin E or a derivative thereof, N-acetyl tyrosine or a derivative thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, citric acid, arbutin , resveratrol, silk extract, plant extracts (chamomile, mulberry, medlar, angelica, mantle, Sophora flavescens, Ai, honeysuckle, Astragalus, Houttuynia, sorghum, alfalfa, wild sesame, fluffy cloth , hawthorn, eucalyptus, eucalyptus, hollyhock, cinnamon, vine, medlar, chicken mulberry, marigold, platycodon, dodder, continuation, shoot, ephedra, Chuanxiong, Duhuo, Bupleurum, wind, Coral vegetables, Astragalus, Cortex, yak seedlings, Pueraria, licorice, gallnut, aloe, cohosh, safflower, green tea, black tea, Axian medicine, etc.

(21) Melanin pigment reduction/decomposition substance phenylmercury hexachlorophenol, oxidized mercury, mercurous chloride, hydrogen peroxide water, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether) Wait.

(22) Promotion of metabolic turnover / cell activating substance hydroquinone, lactic acid bacteria extract, placental extract, ganoderma lucidum extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract , fermented milk extract, plant extracts (aloe, scutellaria, squid, gentian, burdock, purple root, ginseng, witch hazel, bud, hazelnut, wild sesame, medicine, angelica, calendula, sweet tea, small forsythia, Cucumber, thyme, rosemary, artichoke, etc.

(23) astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, heteropolar ore, zinc phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, citric acid (including children) Tea compound) and the like.

(24) Active oxygen scavenger SOD (Superoxide Dismutase), contact enzyme, glutathione peroxidase and the like.

(25) Antioxidant Vitamin C or its salt, stearate, vitamin E or its derivative, n-dihydroguaiac acid, butyl hydroxytoluene (BHT), butyl hydroxyanisole (BHA), hydroxytyrosol , p-hydroxyanisole, propyl gallate, sesame phenol, sesame seed, cottonseed phenol, propolis and the like.

(26) Peroxidative lipid production inhibitor β-carotene, plant extract (flax cultured cells, sweet tea, small forsythia, witch hazel, clove, melissa, marigold, birch, sage, rosemary, southern Real, camp, ginkgo, green tea, etc.

(27) Anti-inflammatory agents fish fat, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetamidine salicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, Hydrocortisone, guaiac, Chamazulene, chlorpheniramine maleate, glycyrrhizic acid or its salt, glycyrrhetinic acid or its salt, licorice extract, purple root extract, commercial extract, Propolis and so on.

(28) antibacterial, bactericidal, disinfectant rivanol, sulfur, calcium gluconate, glucopicic acid dichlorobenzidine, amino sulfonic acid, mercury bromine red, lactoferrin or its hydrolyzate, alkyl chloride Aminoethylglycine, triclosan, sodium hypochlorite, chloramine T, bleaching powder, iodine compound, iodoform, hexadienoic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, p-hydroxybenzoic acid Ester, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-xylenol, p-chloro-m-cresol, resveratrol , phenylethyl alcohol, o-phenylphenol, Irgasan CH3565, haloban, hexachlorophenol, chlorhexidine, ethanol, methanol, isopropanol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol 1,2-pentanediol, zinc pyrithione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, polyoxyethylene nonylphenyl ether, polyoxyethylene octyl) Phenyl ether, etc., amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, potassium lauric acid, etc.), cation Surfactant (bromo cetyltrimethylammonium chloride, benzalkonium chloride, benzethonium chloride, methyl rosyl chloride), formaldehyde, hexamethylenetetramine, brilliant green, malachite green, crystal violet , Germal, photoreceptor No. 101, photoreceptor No. 201, photoreceptor No. 401, N-long-chain mercapto basic amino acid derivatives and acid addition salts thereof, zinc oxide, eucalyptus, matrine, propolis and the like.

(29) humectant glycerin, propylene glycol, 1,3-butanediol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin derivative, water-soluble chitosan derivative, pyrrole Pyridonecarboxylic acid or its salt, sodium lactate, Mini Sasanishiki extract, natto metabolite, natto extract, loofah water, white birch or red pine sap

(30) Elastase activity inhibitor Diisopropyl fluorophosphate, plant extracts (Astragalus, Small forsythia, Sophora flavescens, Mulberry leaves, Cinnamon, Yak seedlings, Confucius, Sage, European elderberry, Linden, Mudan peel), seaweed extract, etc.

(31) hair agent selenium disulfide, alkyl bromide alkyl isoquinolinium sulfonate, thiopyrimidine zinc oxide, jinniuol ester, dimethyl sulfonium, beaver, ginger, capsicum, quinine hydrochloride, strong Ammonia water, potassium bromate, sodium bromate, thioglycolic acid, and the like.

(32) Antiandrogen Egg hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, isoethenone, and the like.

(33) peripheral blood vessels promote blood flow agent vitamin E or its derivatives, drug extract, garlic extract, ginseng extract, aloe extract, gentian extract, angelica extract, head flower ginseng, cape Chloramine, Minidine, etc.

(34) Stimulators Capsicum anthraquinone, vanillyl citrate, cantharidin, ginger, peppermint oil, l-menthol, camphor, benzyl nicotinic acid, and the like.

(35) Metabolic active agent Photosensitive No. 301, eucalyptus, pantothenic acid or a derivative thereof, allantoin, placental extract, biotin, glycerol pentadecanoate, and the like.

(36) Anti-lipids Pyridoxine or its derivatives, sulfur, vitamin B6, and the like.

(37) Keratinolytic agents Resorcinol, salicylic acid, lactic acid, urea, and the like.

(38) oxidant hydrogen peroxide water, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, peroxidation Sodium orthophosphate, sodium citrate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, and the like.

(39) Inorganic reducing agents such as barium sulfate, sodium sulfide, barium sulfide and calcium sulfide; thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, Magnesium thioglycolate, bismuth thioglycolate, and the like.

(40) Hair swelling agent Ethanolamine, urea, hydrazine, and the like.

(41) Dyeing agent 5-amino o-cresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylamino hydrazine, 3 , 3'-iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitro-p-phenylenediamine hydrochloride , p-phenylenediamine hydrochloride, N-phenyl p-phenylenediamine hydrochloride, m-phenylenediamine hydrochloride, o-aminophenol, N-phenyl-p-phenylenediamine acetate, 1,4-diaminopurine, 2, 6-Diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitro-p-phenylenediamine, p-aminophenol, p-nitroorthobenzene Diamine, p-phenylenediamine, p-methylaminophenol, picrylic acid, sodium picrate, N,N'-bis(4-aminophenyl)-2,5-diamino-1,4 - quinone diimine, 5-(2-hydroxyethylamino)-2-methylphenol, N-phenyl-p-phenylenediamine, m-aminophenol, m-phenylenediamine, 5-amino-based phthalate Phenol, 2-amino-5-nitrophenol sulfate, o-aminophenol sulfate, o-chloro-p-phenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diamine sulfate Phenol, toluene-2,5-diamine sulfate, nitro-p-phenylenediamine sulfate, sulfuric acid Aminophenol, p-nitrophthalic acid sulfate, p-nitrom-phenylenediamine sulfate, p-phenylenediamine sulfate, p-methylaminophenol sulfate, m-aminophenol sulfate, m-phenylenediamine sulfate, catechu Phenol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucinol, gallic acid, resorcinol, citric acid, 2-hydroxy-5-nitro-2', 4'-Diaminoazobenzene-5'-sulfonic acid sodium, hematoxylin and the like.

(42) Natural animal flavors such as musk, civet, beaver, and ambergris; anise essential oil, white peony oil, ylang-ylang oil, iris oil, fennel oil, orange essential oil, perfume tree oil, caraway essential oil, Cardamom essential oil, guaiac essential oil, cumin essential oil, fishing rod essential oil, cinnamon oil, Ceylon cinnamon oil, geranium essential oil, Cuba balsam essential oil, alfalfa essential oil, perilla essential oil, cedar essential oil, citronella essential oil, jasmine essential oil , Ginger Essential Oil, Chinese Fir Essential Oil, Spearmint Essential Oil, Peppermint Essential Oil, Anise Essential Oil, Tuberose Oil, Clove Essential Oil, Neroli Essential Oil, Holly Essential Oil, Turul gum Essential Oil, Chloris Essential Oil, Rose Essential Oil, Rose Grass Essential oil, eucalyptus essential oil, arborvitae essential oil, white sandalwood essential oil, lime essential oil, geranium essential oil, ylang ylang essential oil, citrus essential oil, Peruvian scented essential oil, rosewood essential oil, eucalyptus essential oil, citrus essential oil, eucalyptus essential oil, lime essential oil , botanical oils such as lavender essential oil, agarwood essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil; other synthetic fragrance Wait.

(43) Pigment, coloring agent red cabbage pigment, red rice pigment, valerian pigment, annatto red pigment, squid melanin, curcumin, glutinous rice pigment, krill pigment, persimmon pigment, caramel, gold, silver, hazelnut pigment , corn pigment, onion pigment, tamarind pigment, spirulina pigment, buckwheat whole grass pigment, cherry pigment, seaweed pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple sweet potato pigment, purple sweet potato pigment, shellac pigment, rutin Wait.

(44) Other examples include hormones, metal ion blockers, pH adjusters, chelating agents, antiseptic/mold inhibitors, cooling agents, stabilizers, emulsifiers, animal/plant proteins and their decomposition products, and Plant polysaccharides and their decomposition products, animal/plant glycoproteins and their decomposition products, blood flow promoting agents, anti-inflammatory agents/anti-inflammatory agents/anti-allergic agents, wound healing agents, foam boosters, tackifiers, enzymes, Purified water (including electronic water, small clustering, etc.), oral preparation, deodorizing/deodorizing agent, bittering material, seasoning, etc., in combination with these, it is expected that the effect of the present invention or other cosmetic effects may be accumulated or Collaborative improvement. Example

The example of Enterococcus faecalis EC-12 which is the most prominent of the target effects of the present invention is exemplified as the Enterococcus genus Lactococcus, but the present invention is not limited to the examples and the like.

(The storage of the micro-organisms) On February 25, 2005 (the original storage day), the Enterococcus faecalis EC-12 (E. faecalis EC-12) was deposited in the Patent Bio-Reservation Center of the Industrial and Technological Research Institute of the Independent Administrative Corporation (Ibaraki Prefecture) It is the sixth in the central part of the city. The deposit number is FERM BP-10284.

According to a preferred aspect of the present invention, the bacterium used as an active ingredient is a bacterium belonging to the genus Enterococcus. More preferably, the bacterium is Enterococcus faecalis.

Examples of the Enterococcus faecalis include strains of Enterococcus faecalis EC-12 (Accession No. FERM BP-10284), ATCC 19433, ATCC 14508, ATCC 23655, IFO 16803, IFO 16804, or variants thereof. As the bacteria to be used as an active ingredient, the above-mentioned EC-12 is most preferable. In addition, the term "mutant strain" as used herein means that a particular strain is mutated by a manufacturer in a manner that does not change its properties by a method known to the manufacturer, or the operator can confirm that it is equivalent. By.

(Adduction of Enterococcus genus Lactococcus and its heat-treated dead cells) Enterococcus faecalis EC-12 (registered number FERM BP-10284) was purchased from Combi Co., Ltd.

(Production of Plant Polysaccharides) True aloe polysaccharides are produced as plant polysaccharides. After pulverizing 100 g of true aloe leaves, press extraction and filtration, adding ethanol or isopropyl alcohol to the obtained filtrate and stirring, recovering the precipitate, and adding ethanol or isopropanol again and washing The obtained washing material is dried to obtain a true aloe polysaccharide powder.

(Preparation of sample) Samples 1 to 4 (final concentration of the added medium) shown in Table 1 were prepared by mixing the above-produced Enterococcus genus Lactococcus heat-treated dead cells with true aloe polysaccharides. [Table 1]

(Cultivation of Staphylococcus epidermidis standard bacteria) Nutrient broth No. 2 (Nissui Pharmaceutical Co., Ltd.) was used as a medium. Samples 1 to 4 (value in the table: % final concentration of the medium) were added to the medium. Then, Staphylococcus epidermidis standard strain (ATCC12228) was inoculated into the medium in a manner of 1 to 5 × 10 ³ CFU/μL, and the cells were cultured at 37 ° C for 24 hours. Further, the control group was set to heat-treated dead cells (sample 1) without adding Enterococcus genus.

(Collection method of human skin resident bacteria) Using a sterilizing cotton swab, wipe the 3 cm square eyebrows in the longitudinal direction 3 times and the horizontal direction 3 times, and collect the skin resident bacteria. Then, the cotton swab was washed in 10 mL of phosphate buffered physiological saline to collect bacteria.

(Cultivation of human skin resident bacteria) Nutrient broth No. 2 (Nissui Pharmaceutical Co., Ltd.) was used as a medium. The Enterococcus genus Lactococcus was added to the medium to heat-treat the dead cells in such a manner that the final concentration became 0.25, 0.5, 1.0 w/w%. Then, the resident bacteria of the skin collected by the above method were inoculated in 100 μL of the medium. The cells were incubated at 37 ° C for 24 hours. Further, the control group was set to have no heat-treated dead cells (0 w/w%).

(Measurement of Amount of Glycerin) The culture solution of each of the above-mentioned Staphylococcus epidermidis standard bacteria (ATCC12228) and human skin resident bacteria was fractionated. The culture solution was stirred with a vortex mixer, and the amount of glycerin was measured in accordance with a conventional method using a glycerol detection kit (Cayman Chemical).

(Results of measurement of glycerin amount) The results are shown in Fig. 1 to Fig. 2 . The dead cells were heat-treated by Enterococcus genus, and the glycerol production from the Staphylococcus epidermidis standard strain (ATCC 12228) was significantly increased (Fig. 1). Approximately 10 times the glycerol production-promoting effect was observed at a final concentration of 1.0 w/w% (sample 2) in the medium. Further, in the combination with the true aloe polysaccharide (0.1 w/w%) (sample 4), the glycerol production derived from the Staphylococcus epidermidis standard bacteria was synergistically increased. The dead cells were heat-treated by the addition of Enterococcus genus, and an increase in the amount of glycerol production was observed in the human skin resident culture medium (Fig. 2).

(personal observation test) Male and female functional inspectors (subjects: 18 in total) wash their faces before going to bed every day, and apply a soft lotion (lotion A) to the right cheek with heat-treated dead bacteria added with Enterococcus genus For the left cheek, use a placebo soft lotion (make lotion B) that is not added with Enterococcus genus Lactococcus to heat the dead cells. It is used continuously for 7 days (formula is the soft lotion described below), and gets up on the 8th day. Thereafter, each of the cheeks of 3 cm square was wiped by a sterilizing cotton swab in the longitudinal direction three times and three times in the transverse direction, and the glycerin on the skin surface was recovered. Then, the cotton swab was washed in 200 μL of phosphate buffered physiological saline to disperse the metabolite.

(Soft lotion) Enterococcus genus Lactococcus heat treatment Dead bacteria 0 or 0.1w/v% Ethanol 5.0% 1,3-Butane diol 8.0% PEG-60 hydrogenated castor oil 0.2% Perfume 0.01% Hydroxybenzoic acid Ester 0.15% Citric acid 0.02% Trisodium citrate 0.08% EDTA disodium 0.03% The remaining amount of water was set to 100. The above was uniformly mixed according to a conventional method to obtain a soft lotion (molder A, lotion B).

(Measurement of Amount of Glycerin) The above dispersion was stirred by a vortex mixer, and a glycerin test kit (Cayman Chemical) was used, and the amount of glycerin was measured in accordance with a conventional method.

(Results of measurement of glycerin amount) The results are shown in Fig. 3 . In the case of using a soft lotion to which the dead bacteria were heat-treated with Enterococcus genus, the glycerin on the skin surface of the group was observed to be significantly increased as compared with the soft lotion of the control group.

(human observation test faculty evaluation) For the male and female functional inspectors who performed the human observation test (subjects: 18 in total), the feeling of use of the soft lotion used in the test, and the state of the skin before and after the test were performed. Questionnaire. The results of the answer are shown in Table 2. [Table 2]

(Quantitative analysis of human β-defensin-2 (hBD2), human β-3 (hBD3) mRNA) Human epidermal keratinization using a culture medium for epidermal cells (Humedia KG2, manufactured by Kurabo Co., Ltd.) in a 24-well culture dish The cells (manufactured by Kurabo Co., Ltd.) were cultured to a sub-fusion state. The culture medium for inducing differentiation of calcium to Humedia KG2 was further cultured in the presence of 5% CO ₂ at 37 ° C for 3 days, and then transferred to a new medium. The Enterococcus genus Lactococcus was added to the culture medium to heat-treat the dead cells in such a manner that the final concentration became 0.25, 0.5, 1.0 w/w%. The Staphylococcus epidermidis standard strain was added as a positive object so as to be about 1 × 10 ⁴ /mL. After 6 hours of culture, total RNA was extracted from human epidermal keratinocytes using RNeasy (Qiagen). Using the extracted RNA as a template, a reverse transcription reaction was carried out by a conventional method using SuperScript VILO (Invitrogen) to prepare cDNA.

PCR was carried out using the above cDNA. The conditions of PCR were based on the sequence of human β-defensin-2 cDNA (Accession No. NG_023301) and the cDNA sequence of human-β defensin-3 (Accession No. NC_000008), and the primers shown in Table 3 were prepared. The TAKARA Human Housekeeping Gene Primer Set Human ACTB Primer was used as an primer for β-actin used as an internal standard.

[table 3]

Using ^{One Step SYBRR PrimeScript TM RT-PCR} Kit (TAKARA) PCR reaction was performed using TaKaRa PCR Thermal Cycler Dice, real time PCR performed by conventional analytical methods. The amount of mRNA of human β-defensin-2 and human β-defensin-3 and β-actin was relatively quantified according to the above-described real-time PCR analysis.

(Method for evaluating the amount of mRNA of human β-defensin-2 and human β-defensin-3 and β-actin) Percentage of human β-defense against the amount of mRNA as β-actin as an internal standard The mRNA levels of cytokinin-2 and human β-defensin-3 and β-actin were evaluated. Relative expression of human β-defensin-2 and human β-defensin-3 mRNA (% relative to control group) = (human β-defensin-2 and human β-defensin-3 mRNA amount) / (β- Actin mRNA amount) × 100

(Quantitative analysis results of human β-defensin-2 and human β-defensin-3 mRNA) The results are shown in Fig. 4 . The amount of β-defensin-2 and β-defensin-3 mRNA derived from human epidermal keratinocytes was also increased by heat treatment of the dead cells by Enterococcus genus Lactococcus (Fig. 4).

(Examples of various external preparations for skin protection) 1. Sunscreen cream (O/W cream type) (oil phase) 2-methoxyhexyl methoxycinnamate 5.0% 4-tert-butyl- 4'-Methoxydibenzopyrene 1.0% squalane 10.0% Vaseline 5.0% Stearyl alcohol 3.0% Stearic acid 3.0% Glycerol monostearate 3.0% Polyacrylic acid 1.0% Antioxidant Appropriate amount of preservative Proper amount of Enterococcus genus Lactococcus is heated to treat dead cells 0.01-1% (appropriate amount) (Water phase) Purified water The remaining amount of 100 is dipropylene glycol 7.0% titanium dioxide (alumina surface treatment) 5.0% disodium edetate 0.05% triethanolamine 1.0% (manufacturing method) The oil phase and the water phase are separately heated to 70 ° C Further, the aqueous phase portion was sufficiently dispersed in titanium dioxide, and the oil phase portion was added. The emulsified particles were homogenized using a homomixer, and then degassed, filtered, and cooled to be stored. The cooling method is carried out by a method of slowly cooling while stirring a tank into a casing for producing a kettle, a method of cooling by using a heat exchanger, and the like.

2. Sunscreen lotion (W/O type emulsion) (oil phase part) 2-ethylhexyl methoxycinnamate 5.0% Titanium dioxide (hydrophobic treatment) 5.0% Zinc oxide (hydrophobic treatment) 9.0% Angle Squalane 20.0% Polyoxyl 23.0% Polyoxyl 2.0% Glyceryl diisostearate 2.0% Organically modified montmorillonite 0.5% Preservatives Proper amount of appropriate amount of Enterococcus genus Lactococcus 0.01 to 1% (appropriate amount) (Water phase part) The remaining amount of purified water is set to 100% of 1,3-butanediol 5.0% (manufacturing method) The portion and the water phase portion are separately heated to 60 ° C to be dissolved, and the oil phase portion is sufficiently dispersed in titanium dioxide or zinc oxide, and the aqueous phase portion is added, and the emulsified particles are uniformly homogenized using a homomixer, and then degassed and filtered. Cool and store.

3. sunscreen oil p-methoxycinnamic acid 2-ethylhexyl ester 2.0% liquid paraffin 56.0% isopropyl myristate 10.0% polyxanthene oil 30.0% polyoxyl resin 2.0% antioxidant appropriate amount of flavor appropriate enterococci Lactobacillus heat treatment of dead cells 0.01 to 1% (appropriate amount) (Manufacturing method) Add polyoxyxylene resin to polysulfonate to dissolve it, then add isopropyl myristate and stir well, then add the rest. Ingredients and stir.

4. Powder type foundation (powder) Zinc myristate treated talc 20.0% sericin 34.6% titanium oxide 10.0% iron oxide 1.0% iron oxide yellow 4.0% iron oxide black 0.4% titanium oxide/alumina surface treatment mica 15.0% nylon Powder 5.0% Enterococcus genus Lactococcus 0.01 to 1% (appropriate amount) (substrate) Squalane 3.0% Octyl lauryl myristate 3.5% Vaseline 2.5% (Other) Preservatives Appropriate amount (Manufacturing method) The powder components are mixed by a mixer such as a high-speed flow type mixer, and fine particles are further produced by a pulverizer, and then the base material is added, and the mixture is sufficiently mixed using a vertical screw type mixer or the like. Further, after pulverization and granulation using a hammer mill or the like, compression molding is performed.

5. Dual-purpose foundation (powder) 矽 treatment of titanium oxide 10.0% talc 30.0% sericite 39.6% iron oxide 1.5% iron oxide yellow 5.5% iron oxide black 0.4% methyl methacrylate powder 3.0% Enterococcus genus Heat treatment of dead cells 0.01 to 1 (appropriate amount) (substrate) Squalane 3.0% Polydimethyl methoxy oxide 2.0% Triisooctanoic acid glyceride 3.0% Ethyl benzoic acid ethyl ester 2.0% (Other) Preservative Moderate amount of spices Moderate amount

6. Cream (W/O type) (A phase) Third butyl methoxybenzhydryl methane 3.0% Diethylamino hydroxybenzimidium benzoate 2.0% Polydimethyl oxa oxide, octyl Polydimethyloxane ethoxy glucoside 20.0% glycerol tris(behenic acid/isostearic acid/eicosanedioic acid) 1.0% stearyl polydimethyl methoxy alkane 1.0% acetic acid vitamin E Ester 0.05% Benzoic acid 0.3% Diisopropyl sebacate 7.0% Octyl cyanoacrylate 8.0% Talc, iron oxide yellow, magnesium stearate, titanium dioxide, mica, aluminum hydroxide, stearic acid 1.4% (B Phase) Titanium Dioxide, Magnesium Stearate, Talc, Iron Oxide Black, Aluminum Hydroxide, Mica, Stearic Acid 1.13% Red and Brown Iron Oxide, Hard Fat Magnesium, titanium dioxide, mica, water, alumina, stearic acid 0.85% titanium dioxide, talc, magnesium stearate, aluminum hydroxide, mica, stearic acid 3.9% (phase C) water is set to 100 residual magnesium sulfate 0.7% betaine 1.0% sodium hyaluronate 0.1% glycerol 1.0% (D phase) phenoxyethanol 0.4% (E phase) Titanium dioxide, glycerin, alumina 3.5% (F phase) Dimethyl hydrazine alkylation cerium oxide 1.0% (G phase) cyclopentaoxane, (polydimethyl methoxy oxane / vinyl trimethyl decyloxy decanoic acid) crosslinked polymer 1.0% Enterococcus genus Lactococcus heat treatment of dead cells 0.01 to 1 (appropriate amount) (H phase) Flavor 0.3% (Manufacturing method) Mix the raw materials of phase A with stirring, and raise the temperature to 70 to 75 ° C while stirring Add phase B to phase A and stir until homogeneous. While stirring the raw material of the C phase in another container, the mixture was heated to 70 to 75 ° C, and the phase C was added to the AB mixed phase, and the mixture was slowly added while stirring (about 5 minutes), and the phase C was added and stirred for 3 minutes. Then it cools naturally. When the temperature reached 40 ° C or lower, the phase D was added and stirred for 1 minute, the phase E was added and stirred for 5 minutes, then the phase F was added and stirred for 1 minute, and the phase G was added and stirred for 1 minute. Finally, the H phase was added and stirred for 2 minutes, after which it was naturally cooled to room temperature.

7. Moisturizing gel (cosmetic liquid) (A phase) Olive oil fatty acid stearyl ester + sorbitan olive oil fatty acid ester 3.5% olive oil unsaponifiable 0.5% di-n-octyl carbonate 3.0% avocado oil 0.5% Wheat Germ Oil 0.5% Cyclodecyl Ester 6.0% Polydimethyloxane 1.0% (Grade B) Water is 100% Residual Glycerin 1.5% Propylene Glycol 1.5% Sodium Polyacrylate 0.7% Trister Gum 0.1% (C Phase Preservatives Menthol amount 3.0% 0.3% ethanol, 0.01 to 1 Enterococcus (qs) (Production Method) Lactococcus lactis heat treated dead cells were prepared the A phase and B are mixed. By dispersing the Sanxian gum and sodium polyacrylate by using a batch type disperser or the like, the phase A is added to the phase B at 75 ° C, and after a few minutes of homogenization, the mixture is slowly stirred while being cooled, and after adding 40 ° C, C is added. Phase (mentholm pre-dissolved in ethanol).

8. Lipstick (A phase) Mineral oil 8.0% Red pigment 1.0% Yellow pigment 0.3% Enterococcus genus Lactococcus 0.01 to 1% (appropriate amount) (B phase) Triglyceride (octanoic acid/capric acid) ester is set to 100 residual amount Ethylhexyl methoxycinnamate 3.0% Isopropyl myristate 10.0% (C phase) Alkyl (C26-28) polymethyloxane 9.0% Cetyl alcohol 1.6% (D phase) PEG-8 , vitamin E, ascorbyl palmitate, ascorbic acid, citric acid mixture 0.1% propylparaben 0.1% (E phase) perfume 0.2% mica, titanium oxide mixture 18.4% mica, titanium oxide, iron oxide mixture 7.0% cerium oxide 0.5% (manufacturing method) The phase A is uniformly premixed, added to the phase B and uniformly mixed, and the phase C is further added, and the mixture is heated to 78 while stirring slowly. After the temperature was changed to 80 ° C, the D phase and the E phase were added in portions, and the mixture was uniformly stirred, and then poured into a mold to be naturally cooled to normal temperature.

9. Foundation (O/W type) (oil phase) Stearic acid 2.4% Propylene glycol monostearate 2.0% Cetearyl alcohol 0.2% Liquid lanolin 2.0% Liquid paraffin 3.0% Isopropyl myristate 8.5% oil-soluble preservative proper amount (aqueous phase) Purified water set to 100 remaining amount of sodium carboxymethylcellulose 0.2% bentonite 0.5% dipropylene glycol 4.0% triethanolamine 1.1% water-soluble preservative proper amount (powder) Titanium 8.0% Talc 4.0% Coloring pigment Appropriate amount of Enterococcus genus Lactococcus heat treatment Dead bacteria 0.01 to 1 (appropriate amount) (Others) Flavor amount (manufacturing method) After pulverizing the powder, add the water phase and mix it while stirring The oil phase and the fragrance were added thereto, and the mixture was emulsified and dispersed by a homomixer, and then cooled to room temperature.

10. Cream foundation (W/O type) (oil phase) Decamethylcyclopentaoxane 15.0% Liquid paraffin 5.0% p-methoxycinnamate 3.0% Polyether modified dimethyloxane 2.0% (Aqueous phase) Purified water set to 100 Residual amount of glycerin 5.0% Water-soluble preservative appropriate amount (powder) Titanium oxide 10.0% Talc 3.8% Coloring pigment appropriate amount Enterococcus genus Lactococcus 0.01 to 1% (proper) (Other) Spice Moderate amount

11. Moisturizing cream (O/W type) (A phase) Shea butter 1.5% alkyl benzoate (C12-15) ester 1.3% alkyl (C26-28) polydimethyl oxirane 6.5% Hard Glycerylglycerol 1.5% Stearic acid glyceride, stearic acid PEG-100 mixture 3.0% hexyl laurate 1.3% isododecane 1.3% isohexadecane 1.3% isopropyl palmitate 1.3% Vitamin E ester 0.5% Enterococcus genus Lactococcus heat treatment of dead cells 0.01 ~ 1 (appropriate amount) (B phase) Purified water set to 100 residual amount of carbomer 0.3% glycerol 2.0% propylene glycol 2.0% (C phase) Perfume 0.5% Phenoxyethanol, methyl paraben, ethyl p-hydroxybenzoate, butyl p-hydroxybenzoate, propyl p-hydroxybenzoate, p-hydroxybenzoic acid Butyl ester mixture 0.5% tetrahydroxypropyl ethylenediamine 0.7% (Manufacturing method) After heating phase A and phase B to 70 ° C in different vessels, phase A is added to phase B, using batch type disperser After homogenization, it was naturally cooled to 40 ° C while stirring. Then, the phase C was added thereto, further homogenized, and cooled to room temperature. [Industrial availability]

The Enterococcus genus Lactococcus of the present invention can promote the production of glycerol derived from Staphylococcus epidermidis which is a resident skin-preserving skin and the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin. Moreover, when applied to the skin, it is very dry and very excellent in touch, and it can provide good sensibility to any of the aqueous, oily, emulsion, and powder-based external preparations. Therefore, the use of the agent for normalizing the skin condition or protecting the skin which maintains normalness and the external preparation for skin protection are extremely high.

Fig. 1 shows a heat-treated dead cell of Enterococcus faecalis EC-12 and a true aloe polysaccharide as a plant polysaccharide added to a culture solution of Staphylococcus epidermidis standard strain (ATCC 12228), and glycerin The amount produced is quantified to obtain the result. Fig. 2 shows the results obtained by adding the heat-treated dead cells of Enterococcus faecalis EC-12 to the culture solution of the skin resident bacteria collected from the human eyebrows, and quantifying the amount of glycerol production. Fig. 3 shows the results obtained by quantifying the amount of glycerol collected from human cheeks in a human observation test using a soft lotion containing heat-treating dead cells of Enterococcus faecalis EC-12. Figure 4 shows the culture of a human normal keratinocyte culture medium in a medium containing heat-treated dead cells containing Enterococcus faecalis EC-12, extracting RNA from a human normal keratinocyte cell line, and measuring human β-defense by real-time PCR. The results obtained by expressing the amount of ubiquitin-2 (hBD2) and human β-defensin-3 (hBD3) mRNA.

(no)

Claims (12)

An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, which comprises Enterococcus genus Lactococcus as an active ingredient. An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis according to claim 1, wherein the Enterococcus genus Enterococcus faecalis is Enterococcus faecalis. An accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis according to claim 1 or 2, wherein the Enterococcus genus is Enterococcus faecalis EC-12 (registered number FERM BP-10284). The accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis according to any one of claims 1 to 3, wherein the Enterococcus genus Lactococcus is a dead cell. The promoter for promoting the production of glycerol derived from Staphylococcus epidermidis according to any one of claims 1 to 4, wherein the Enterococcus genus is a heat-treated dead cell. An accelerator for promoting the production of an antibacterial peptide derived from epidermal keratinocytes of the skin, which comprises Enterococcus genus Lactococcus as an active ingredient. An accelerator for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin of claim 6, wherein the Enterococcus genus Enterococcus faecalis is the above-mentioned Enterococcus faecalis. The promoter of claim 6 or 7 which promotes the production of an antibacterial peptide derived from cutaneous keratinocytes of the genus Enterococcus faecalis EC-12 (Accession No. FERM BP-10284). The promoter for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin of any one of claims 6 to 8, wherein the above-mentioned Enterococcus genus is a dead cell. The promoter for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin of any one of claims 6 to 9, wherein the above-mentioned Enterococcus genus is a heat-treated dead cell. The antibacterial peptide production promoter according to any one of claims 6 to 10, wherein the antibacterial peptide is human β-defensin-2 (hBD2: human β-defensin-2), human β-defensin-3 ( hBD3: human β-defensin-3). An external preparation for skin protection, which comprises the accelerator for promoting the production of glycerol derived from Staphylococcus epidermidis, and the promoter for promoting the production of an antibacterial peptide derived from cutaneous keratinocytes of the skin, according to any one of claims 1 to 11.