JP2002020300A - Cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To study a method for using a yeast widely useful for human beauty and health, having a higher additional value while directing attention to a metabolic ability of yeast and a component contained in its microbial cell. SOLUTION: The hydrogen peroxide eliminant, the antiinflammatory agent and the cosmetic composition are characterized by comprising a yeast extract having >=0.0001 wt.% calculated as solid content of glutathione content by culturing a yeast of the genus Saccharomyces. Consequently, the hydrogen peroxide eliminant and the antiinflammatory agent have excellent in hydrogen peroxide eliminating action and anti-inflammatory action and have extremely improved utility and usability as a cosmetic use. The cosmetic composition formulated with the hydrogen peroxide eliminant and the antiinflammatory agent can ameliorate chapped skin/dry skin and provide the skin with gloss and firmness and can be expected to prevent aging phenomena caused by active oxygen.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to the genus Saccharomyces
(Saccharomyces) It is intended to provide a hydrogen peroxide scavenger, an anti-inflammatory agent, and a cosmetic composition using the same, which contain a glutathione-rich yeast extract obtained by culturing yeast.

[0002]

2. Description of the Related Art Yeasts belonging to the genus Saccharomyces, such as brewer's yeast, sake yeast, wine yeast and baker's yeast, are microorganisms that are very closely related to human food culture. The history of taking advantage of the nature of and taking advantage of it is extremely old. Speaking of foods produced by the action of these yeasts in the body, for example, alcohols, bread, miso, soy sauce, pickles and the like are typical ones. Well, in addition to utilizing metabolites produced by such biological action (fermentation action), the use of yeast is also known to utilize the components contained in bacterial cells. ing. In other words, yeast contains abundant and well-balanced nutrients such as nucleic acids and their related substances (RNA, ATP, etc.), proteins, various amino acids, vitamins (particularly group B), and minerals. Yeast and its extract have been used as protein resources, and have also been awarded as health drugs for the purpose of nutrition, tonicity, health recovery promotion, intestinal control, and strong liver.

[0003] In recent years, it has been recognized that such components in yeast are very good for cosmetics, and yeast extract or yeast which has been used for food or the like has been hydrolyzed. The obtained extract has been applied to various cosmetics.
For example, JP-A-53-142515, JP-A-60-24188, JP-A-61-260009, and the like, based on the cosmetic effect of yeast extract or yeast hydrolyzed extract, the application to cosmetics This is the proposed invention of the earlier application.

[0004] On the other hand, glutathione is a tripeptide composed of amino acids of glutamic acid, cysteine and glycine in vivo, and is widely distributed not only in animals and higher animals, but also in microorganisms and molds, and has various physiological activities. I have. In humans, it is contained abundantly in the liver, and in addition to detoxification, has an antioxidant effect, stores sulfur-containing amino acids, and acts as a synthetic material for biological components. It is also applied to various cosmetics, and Japanese Patent Application Laid-Open Nos. 62-283909 and 01-84668 are inventions of the prior application for glutathione skin and hair / scalp.

[0005]

SUMMARY OF THE INVENTION The present inventors have focused on the metabolic ability of yeast and the components contained in the cells of the yeast, and have developed a yeast with higher added value that is widely useful for human beauty and health. Consider usage.

[0006]

As a result, a yeast extract having a glutathione content of 0.0001% by weight or more as a solid content, which is metabolized by culturing Saccharomyces yeast, is converted into a normal yeast extract. Compared with hydrogen peroxide scavenging and anti-inflammatory effects,
It has been found that the usefulness and usability for use in cosmetics have been remarkably improved.

In addition, not only extracts obtained by simply disrupting the cells with a French press or the like, but also components which are extracted after the yeast is once autolyzed, or hydrolyzed by an acid or a protease. Thus, the present inventors have found that the same effect can be obtained with any of the obtained extracts, and completed the present invention.

[0008]

DETAILED DESCRIPTION OF THE INVENTION The yeast used in the present invention is a yeast belonging to the genus Saccharomyces, such as brewer's yeast, sake yeast, wine yeast, baker's yeast, and the like.

[0009] The medium for culturing the yeast is a carbon source,
It contains a nitrogen source and, if necessary, known micronutrients. More preferably, glutamic acid, cysteine and glycine are added to the medium. First, as the carbon source, conventionally known various materials can be used, and examples thereof include glucose, molasses, and soluble starch as typical examples. Also, there is no particular limitation on the nitrogen source, yeast extract, peptone, meat extract, corn steep liquor,
Organic nitrogen such as soybean meal and inorganic nitrogen such as ammonium sulfate, urea, ammonium nitrate, and ammonium chloride can be easily obtained.

[0010] Thus, the medium used in the present invention is, for example,
0.5% yeast extract, 0.5% peptone, 0.5% glue
Course, 0.1% K_TwoHPO_Four, 0.02% MgCl_Two・ H_TwoMedium containing O
0.5% glycine, 0.25% cysteine, 0.5% glycine
It can be supplemented with glutamic acid, and yeast growth is
It can be carried out in a single or continuous manner. Other culture strips
Issues, such as temperature and time, are generally
However, the culture period should be stationary.
It is desirable to be cheap. Predetermined by culture in the above method
In addition to obtaining yeast, it is usually commercially available as glutathione-rich yeast
Yeast that has been used can also be used. At this time, the yeast
May be dry yeast or pulverized material in addition to live yeast.

The yeast extract used in the present invention is obtained by extracting from yeast or yeast degradation product by the following method, and has a glutathione content of 0.0001% by weight or more as a solid content metabolized by culturing. Use something. still,
Preferably, a solid content of 0.001 to 2.0% by weight is used.

In extracting the active ingredient from the cultured yeast cells, the cells after cultivation are collected, washed thoroughly with purified water or the like, and then disrupted by, for example, ultrasonic waves or a French press. An aqueous solvent such as water, ethanol, butylene glycol, propylene glycol, or glycerin alone or a mixture thereof is added to the wet cells for 10 minutes.
It is sufficient to add about 100-fold volume, perform filtration such as centrifugation, and then filter.

In the case of utilizing the autolysis of yeast, about 10 to 100 times the amount of purified water is added to the washed wet cells, and 35
After autolyzing at -45 ° C for 24-72 hours, freeze-dry or concentrate under reduced pressure. To this, an aqueous solvent is added in the same manner as described above, and after extraction, an operation such as centrifugation is performed, followed by filtration.

When using the enzymatic decomposition method, about 10 to 100 times the amount of purified water is added to the wet cells after washing, and about 200 to 500,000 units of protease are added to 1 kg of yeast cells. The reaction is allowed to take place overnight at the enzyme activation temperature. Next, after inactivating the enzyme, filtration is carried out by an operation such as centrifugation with or without the addition of an appropriate amount of an aqueous solvent.

When the acid decomposition method is used, about 10 times the volume of 1-5% hydrochloric acid is added to the wet cells after washing, and the mixture is added at 40-60 ° C.
Hydrolyze for 3-8 hours with occasional stirring. Then, after neutralization using an alkali, the same treatment is performed. After neutralization, desalting may be performed by dialysis treatment or the like.

Furthermore, those extracted or prepared with a solvent from these yeast degradation products can be used. As an extraction solvent, an organic solvent such as water, ethanol, butylene glycol, propylene glycol, or glycerin can be used alone or in any combination of two or more kinds. It can also be used.

These extracts can be used in many cases as they are in the form of an extract. However, if necessary, purification treatment such as deodorization and decolorization can be performed within a range that does not affect the effects of the present invention. is there. In addition, as a purification treatment means such as deodorization and decolorization, an activated carbon column or the like can be used.

The content of the yeast extract in the hydrogen peroxide scavenger, anti-inflammatory agent, and cosmetic composition of the present invention may vary slightly depending on the type of applied dosage form, the method of use, and the expected degree of action. Usually 0.001 to 10%, preferably 0.
About 01-1.0% is good.

The hydrogen peroxide scavenger, anti-inflammatory agent, and cosmetic composition of the present invention can further optionally select the following components and additives as long as the effects of the present invention are not impaired.
It can be manufactured in combination.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )etc.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid And synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or an ester thereof, trehalose or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) to which an alkylene (C2-C4) oxide such as ethylene oxide is added. )
Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose , Carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide Or its crosslinked polymer, carboxyvinyl polymer, polyethylene N'imin like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or its derivative, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc., and their sulfates, phosphates, nitrates, citrates,
Or amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, powdering, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but usually, water or a water-soluble organic solvent (for example, ethanol) ,Propylene glycol,
It is desirable to use one or a mixture of two or more selected from 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application,
Only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in any mixed solution with water, or may be extracted and extracted.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress by fragrance, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, relief of fatigue, promotion of blood flow,
In addition to cosmetic effects such as warm bath effect, fragrance, deodorant, thickening,
Antiseptic and buffering effects can also be expected.

In foods and drinks, nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and other healthy and cosmetic effects, improvement of taste, provision of color, aroma, gloss, stabilization, It can be used for the purpose of thickening, preserving, etc. Furthermore, in addition to this, we expect various cosmetic and pharmacological effects of each raw material known so far, and aim to increase the intended effect by combining them,
Alternatively, it is possible to provide a product that is expected to have a multifunctional effect.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsujirafuji (Kibo), Acacia, Akashoma (Akamasa), Akasuri `` fruit '', Red clover, Red grape, Akaminoki (Logwood), Red moss (Red bud), Red moss (Akane grass root), Red moss Ground yellow), Agi (Awei), Aki Larch, Akinire (Latinaceae),
Aguay Guacu, Aguahe (Omitengu palm),
Akebi (Kidori), Asa (Jin Asako), Malva Asagao or Asagao (Toshibushi), Hydrangea (hydrangea), Ashitaba (Asahi),
Azuki (red azuki), acerola, asenyaku (Asenyaku), acira (shokuyoukanna), anise, abiu, abiurana, rape, avemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaworu, amadocoro (tamatake), Amana (Kikomiko), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriacus, Amigasa lily or Bimo (shellfish),
Aristolomelia (Yurizisen), Arcana (Alcanna), Algarrobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souduq), Aloe (Length), Aloe vera, Angelica, Apricot or Hong apricot (Anjin), Ansokkou (Azusa), Yellow sapote , Ikakozorina (ground bile head), Epimedium or Epimedium
(Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root),
Strawberry (first place), strawberry, fig (Fig.
Leaf)), Ichihatsu (first time), Ichibi (Fruit Aoi), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Carob, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, Turmeric (Utsukin), Duckweed (Floating), Usubasaishin or Keirinsaishin or Shousaisaishin (Spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qang, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Vladimir, Owaurushi (Kusaco peach), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakisakirengiku) , Enishida, Enokitake (Enotake), Ebisugusa or Cassia Tora (Kyoko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Solanaceae, Sophoraceae rice), Ogi or Kibanaugi (yellow) Hoki), Ousei (Naruko Yuri, Kagi Kurumabanaruko Yuri), OuHisasa Yuki or Sasanoyuki, Coptis (Coptis), Oogatahouken, giant Karasuuri (Karokon), Ooguruma (civil engineering incense), giant Tsudzurafuji
(Self-defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), toucan cactus, obanana okera or okera
(White jujube), giant sedge beetle (banaba), giant beetle "fruit", giant squirrel (cereal sperm), giant caterpillar, hawthorn or hawthorn (mountain sprouts), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', oguruma (turnover), panax ginseng or ginseng (carrots), hypericum or lycopodium perforatum or periwinkle or hypericum perforatum (branch cutting), dragonfly (continuous), onamiomi Ears), Onigurimi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tenma), Oniyuri or Sasayuri or Hakatayuri (Yuri), Ononis, Halibut
(Hihayu), Ominaeshi (defeated), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, pericarp", silkworm (ripened yellow) , Cocoa "fruit, peel,
Seeds '', oysters (persimmons `` leaves ''), kagi kazura (crop hooks), oysters or kantosou (rensensou), gaku hydrangea, cassia, casino ki (crocodile fruit `` fruits ''), gajutsu (shadotsutsu), Kashiwa
(Sakura tree, Sakhalin), Kascarilla, Kascara sagrada, Gypsophila, Kasensou, Kanixa (Kinosato), Kaniwa, Carnation, Kanokoso (Yoshikone), Birch or Shidare kamba (White birch), Gerbera, Pumpkin, Capoknoki `` Seeds '', Kahoku Sansho (shopper), Gama (bamboo), Chamomile or Roman chamomile, Camellia japonica (weed), Camu cam (camouflage), color, Karaku Sakeman, Ragwort or Chinese Ragwort (Our melon), Ragwishak (midsummer), Ragwort, Guarana `` Seeds '', Karaho (thick), Karaya, Galu, Karin (Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Seiryuna, Hakubaku), Kawazusha, Kawatake, Kawaranadeshiko (Ishitake) or Ezokawaranadeshiko ), Japanese carrot (Aoko), Japanese sagebrush (Inchinko), Kansui (Kansu), Licorice Kantarasa, Candelilla, Kanto, Canna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Nawashiroi strawberry, Maple strawberry, European strawberry), Kiwi `` Fruits, leaves '', Kiwi squirrel (Uroro root), Kikyo (Kikyo, Kikyo root), Kiku (Kikyo) Chrysanthemum flower, oak daisies, artichokes, chrysanthemum, catalpa (Azumizumi), rosacea (yotei), pheasant (kokomi), kizuta, kidachi aloe, edachi mint, kina, chinoki (shincona, red linden), quinoa (quinoa), yellowfin ), Gymnema Sylvester,
Kimimaku, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja saponaria, Kiraya, Kyransou (gold acne grass), Kumquat `` fruit '', Ginkai, Kimbusen, Kinma, Kinmizukiki
(Sengaku grass), guapeba vermelilla, guava `` fruit '', guayule, quelux infectria (gallicate), kukui nuts, wolfberry (glygi, gugiko, gugi leaf, skeletal bark), kusagi-kazura (tenmon winter), kuzu (kuzukane) ), Camphor tree, gooseberry `` fruit '', ginseng, yellowtail, gardenia, gardenia, blackberry,
Kupuas, Kumazasa, Kumatsuzura (horse whipweed), Clara (bitter butter), cranberry "fruit", chestnut "seed, fruit, astringent skin", curculigo latifolia "fruit", grapefruit "fruit / leaf", buckthorn, black stalk
(Rescue rescue), Kurobana Hikikoshi or Hikikoshi (extended life grass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi), kaigai (infested, infested ears), celosia
(Cow flower, rooster), geccavidin, bay (laurel), kenashi sardine rose (Tsubukuryo, Yamakiko), gentiana, gennoshoko (Older's grass), kenponashi (Kigushi),
Koukisekoku, Kishumitsukan (Koji, Tachibana,
Olivenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana skin)), Koushinbara (Monthly flower), Kosuui mint, Kozo `` fruit '', Kocha (tea), Kohone
(River bone), kouhon (straw and kara straw), kouriang, kourikyo (takara kang), cilantro `` fruit '', koren
(Hou Lian), Scutellaria (Yellow Gon), Cowberry (Koshibachi), Coco Palm `` Fruit '', Goshuyu (Goshuyu), Goshostrawberry (Uboniko), Pepper (Hot Pepper), Cosmos, Copaiba Balsam,
Coffee `` seed, leaf '', kobushi or magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame hahagusa (gensen), goji (gomiko), Sanekazura or binankazura or Matsubusa, wheat (wheat) , Rice or rice bran “red bran, white bran”, rice oil, cola acuminata “seed”, cola vera “seed”, colony llah, fenugreek “fruit”,
Colombo, Konzulango, Kombu, Konjac, Comfrey (finnari grass), Sisal (Sisal Asa), Sakisima button or Sinata button or Shina sensou (Eisensen), Sakura (Oshimashima cherry, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyayamazakura, Somei Yoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura `` leaves, flowers, fruits, bark (cherry bark) ''),
Cherries, pomegranate, sasa, sasanqua, sagemodaka
(Sawashatsu), sweet potato, sugar cane, sugar beet, sapling jujube (acid jujube), saffron (safflower, west safflower), sapodilla, sapotea marillo, pomelo `` fruit '', savonso, salmon berry `` fruit '', sarasinachoma (masuma), Salvia (sage), Sawagyoukyo (Yamakosai), Sawagumi (Yamakomo), Sankaku cactus, Sangre degrad (Croton), Sansouyousou, Sanshin carrot
(37 ginseng), sanshuyu (sanshuyu), sansho (sansho),
Sandscon (Yamazone), Shea (Karite), Cyanoki `` fruit '', Shiitake (Shiitake), Shion (Shion), Dikitaris, Shikunshi (Kimiko), Perilla or Aozo or Chirimenjiso or Kamenjiso (Shiso leaf, Shiso), Rosewood, linden,
Japanese pine, Chinese forsythia (forsythia), shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiiroshimeji,
Bunashimeji, honjimeshi, shirotamogitake), shimokuren (hot spring), shimotsukeisou, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), shukukonemisou, shukushamitsu (sunin, Shredded sand), juzdama, palm `` fruit '', ginger (ginger), giant hydrangea (Tsuneyama), shove (iris, iris root), shojiku `` fruit '', shirakashi `` seed '',
Shirogou butterfly `` seed '', white clover (clover), white adzuki bean (chicken bone grass), white iris (small iris), white natto `` flower '', white nannan (minamitenmi),
Cinchona Sacrifura, Jinjuge (Mizuka, Mizuka,
Cinnamon), cinnamon, cinnamomum cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flowers, shinto winter), sorrel (acid model), swimberry "fruit", star apple, stevia, strawberry "fruit", tin shiko ,
Horsetail (Thorn), Purslane (Horse Tongue, Hen Tonen), Plum (Fruit), Pinus sylvestris (Cones), Larix sylvestris, L. sylvestris, L. truss, L. hawthorn, L. dandelion, Horse chestnut (Maloney), Pear "fruit", snow pear,
Sambucus elder (Elder), Atlantic shrimp (Juniper, Juniper), Yarrow (milfoil), Rose of the valley, Gourd in the field, Mistletoe littoralis, Common mint or Common mackerel, Common horseradish, Common squirrel (Iris), Cedron (Bowshukuboku), Zeniaoi, Hirohasenega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shingenren), Sendan, Sennin Cactus, Senna `` Fruits, Leaves '', Senninsou (Otatate), Senbri ), Souka (grass), elephant, rosacea, buckwheat `` seed seed '', soybean potato, rhubarb (daisou), radish, soybean, daidai (orange skin, kikomi), takasaburo (dry lotus grass), takatodai (daigo), takawarabi (Inu), Dark Sweet Cherry "Fruit", Tachioi, Tachiji High-rise (time,
(Marika), Tachidokoro (Hikai), Tachiyanagi, Tamarind `` seed '', onion, tamshiba (spicy), Taranoki `` fruit, leaf, root bark '', dahlia (tenjikubotan), tarwi,
Tanshiniwa, Tanjin (Dan ginseng), Dandelion (Gankoei) or Shirobana dandelion or Moko dandelion, Danmar, Cherry `` fruit '', Tigaya `` fruit, root, bud '', Chixet carrot (Bamboo ginseng), Chicory, Chancapiedra (Kidachiko Mikansou) , Datura (Rhuanghuang), Datura elm (Wuyi), Datura mugwort (Aiba), Choreimaitake (Irei), Chorogi, Tsukimisou, Tsukuritake (mushroom), Camellia, Camellia, Tsumekusa (lacquer primrose), Camellia Ducklings), Tsuruazuki (red azuki bean), Tsurukudami
(Hanshu crow), Tsuruna (Ban apricot), Crucian carrot (Four leaf ginseng), Tsuwabuki, Deiko, Teuchigurumi, Duberry `` Fruit '', Tulip, Chocolate stay, Tengusa, Tencha (Tencha), Tendaiyaku (Wood medicine), Touga (Winter ginger) ), Squash, capsicum (pepper), squid (return), calendula (marigold), tonabena (river cut), corn or corn hair (southern hair), horned beetle (female child), torindo or kosen Gentian,
Tokusa (wood pirates), dokudami (jujutsu), konkon (sore root), sashimi or mamedaoshi or scutellaria, eucommia (mushroom `` bark, leaves, roots ''), ash (qin bark), tomato, tragacanth, triacanthos `` seed '', Turkey Kikyo, Tormentilla, Dronoki, Troloa oy, Niseria berry "fruit", Chinese yam or Yamanoimo (Yamayaku),
(Butcher Bloom), Naginata Kouju, Nazuna, Rape, Natsume Bean or Hatehaki (Sword Bean), Natsumikan, Jujube (Daijutsu), Naniwaibara (Kanakurako), Navena (intermittent), Nameko, Narco Lily (Yoshimi), Peanut (Peanut) ), Nanten (Minami Tenmi), Namban Karamushi (ramie), Niois violet,
Nigaki (bitter tree), bitter mugwort (bitter), nutmeg, kei or nikkei or ceylon nikkei or yabnikkei (cinnamon bark)
Or Keishi (Keishi), Leek (Nirako), Elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nulde (fivefold), leek, nemoki or neb or nebi or nemlinoki or zigokubana ( Healing), thistle (daiji), noibara (real fruit), nozuka quail (ryohana), nogateto, yarrow, nodoke (maehu), nobara, peach, pineapple `` fruit '', hibiscus (bussouge, hurinbussouge, roselle), hayindow, Hauchiwa, Pau Doce, Hakamauraboshi (bone crushing), Hakusen (ringworm skin), Huckleberry `` Fruit '', Hakobe (proliferation), Hashibami (Haruko),
Hasiridokoro (roth root), basil, lotus (lotus, lotus, lotus root), parsley (holland jelly), naked mussels, batata, hachik or madake (bamboo-boiled), patchouli, mint (light-weight, light-weight leaves), pearl barley (yokunin) , Hanasuge (Chimo), Banana, Hanamint, Hanabishi (Siturishi, Shitsuturi), Vanillabins, Papaya, Scotch (Rice koji), Pahalobobo, Hub `` whole plant, stem, leaf '', Paprika, Hamago or Hitsuba Hamago Child), Hamasuge (Kazuke), Hamabishi
(Ritsuko Riko), Hamanas (maikai flower), Hamabofu (beach storm), Hamamelis, palm, rose (rose), valata,
Balata rubber tree, agaric mushroom (aratake mushroom, white agaric mushroom, usukimori no kasa), haran, pariurus (prunus edulis), parietalia, balsamina (tsurureishi, bitter gourd), harunire (yellow bark, yu white bark, yule leaf), barbasco, harindou, handaikai Bamboo tree, breadfruit, giant gourd (radish), lycopodium carrot (party), pecan nut, giganbana (garland, vinegar), higuru madaria, haishi (hishomi), pistachio, beet, hitoshiba (stone reed), Aedes aegypti, daisies (daisy), hinata nokozuchi (cow knees), hinoki, hiba, castor, sunflower,
Bell pepper, Himeuzu (Tamaoi), Himegama (Kaba), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta `` Fruit '', Juniper, Biancucuum, Hyacinth `` Fruit '', Belowudo Oi, Hiroha Okinagusa (Hakuto Okina), Loquat `` Fruit,
Leaves, stems ”, betel nut (large clothing skin, betel nut), puer tea (
Tea), spruce buds (southern rattan), butterflies, coltsfoot (populations of winter flowers, copulations of winter leaves), fujibakama (orchids), fujimame (flat beans),
Fujimodoki (choujizakura, shikahana), grapes `` fruit, peel, seeds, leaves '', beech, Fuyumushinatsukutake (Cordyceps sinensis), Brazilian liquorice, Brazilian carrot, black currant `` fruits '', blackberry, blanin,
Plum `` fruit '', flucellaria, blueberry (Prunus avium), prune, floral blanka, blond psyllium, bundou (green bean), hazelnut, loofah, safflower (safflower), henneken, belladonna, berry `` fruit '', Persair, Perbiamberg, Pereskia
Grandifolia, Benisium or duckweed (Keiten), Boysenberry `` fruit '', Broomweed or Elderweed or Nendow or Hawakigi kokia (skin), Baldwink (Hosen, acute child, osteophyte), Potteria sapota, potteria Lucuma, spinach, boufu (windbreak), spinach, physalis (Torone), honoki (Japanese thick, park), bokeh
(Gourd), Hosoba Aburagiku (bitter gourd), Hosoba Oguruma,
Hosovanaokera (Aojutsu), Hosobanokirinsou (Kageten 37), Hosobabarengiku, Bodaiju (Bodaiju), Button
(Peony `` flowers, leaves, stems, bark ''), buttonpies (peony bark), hops, jojoba, borage (ruriichisha), bordeaux, white berries `` fruits '', hon-onik (meat porcelain, daigei), hon-sekko (iron Skin stone mackerel, sparrow mountain stone mascara), myzurutennanshaw (Tenan star), maitake (maitake), ephedra (mao), maca, macadamia nut, margaret (mokshungiku), macri (seaman grass), maguwa (mulberry white bark) `` Bark, leaves ''), Magnolia splengeri, Masaranpouba, Masaranzuba do Ceara, Masaira de Boi, Masahua (Tamanouzenhallen, Cucumber kinrenka), Matatabi (Kiten Tate), Matsukasa, pine `` leaves, bark, Root, Matta Oryo, Matsuhodo (Bukuryo), Matico (Cordoncillo), Mayorum (Hanamint), Malvanojazine (Bittersweet), Mulberry "Fruit", Quince, Murray (Below mou watermelon), mango, mangosteen, mansagna (andesukamitsure), mansugurumi, mandarin `` fruit '',
Mannaketake (Lingzhi), Pheasant (Kikomi `` fruit ''), Mishimasaiko (Saiko), Mizuobako or Mizuasagao (Ryugoshi),
Mizokokushi (half-edge lotus), Mihohagi (Chisatsuna), Mycian willow or Japanese willow (Henryoku), Mitsugashiwa, Mitsuba, Midorisapote, Midorihakka, Mimosa, Miyoka, Miracle fruit `` fruit '', Myrrh, Mirobaran, Mugiwaragiku, Mukuge (Mokuge) , Mukunoki, Mukuroji (prolonged skin), Munya, Purple (purple root), Purple or purple
(Purple pearl), purple corn, purple purple (Kunming chicken blood wisteria), megakanza opuntica, mehajiki (mother grass), mebougi, melaleuca, melissa, melilot,
Melon `` fruit '', sagebrush, moso bamboo, moku giraffe, moje (koshoboku), mock berry (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), bean sprouts, morello cherry `` fruit '', Moroheiya (Burlap), yakawamurasaki potato, yakuchi (masuti), cornflower mackerel (cornflower), cornflower mint, yacon, yashabushi (arrow wheel) or himeyashabushi or giant yashabushi `` fruit,
`` Peel, ears '', willows, yatsude (octagonal gilded), mistletoe (parasitic willow), willows (kawayanagi, tachiyanagi, weeping willow, red willow, pussy willow, dogwood willow,
(Sweet willow, koreanagi, unryuyanagi, yamayamayanagi, palm, yamayanagi, giant psyllium, tyrichkinuyanagi, foxwillow, bonsai), willow `` leaf,
Stalks ''
(Tsurusu, Tenmei), pokeweed (commercial land), yamanoki (yamaharu), yamamomo (yangbai skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu `` fruit '', lily, yorigusa , Mugwort (Aiba), reigan (Rainuma), lime "fruit", rye, lakanka "fruit",
Raspberry `` leaves, fruits '', latania (Clameria), Rakkyo or shallots (Shiroi), lavender, longan (longan meat), agave (Aono agave,
Fukurin agave), Ryokucha (green tea), apple `` fruit, seeds, leaves, roots '', gentian, rubus or suavisimus (sweeten), litchi (lichi, lichi nucleus), lettuce (chisha), red currant `` fruit '', Red pitaya, lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, wax bye, wax palm,
Lokon (reeds and radishes), Loganberry "fruit", rosemary (mannan wax), rose hips (nobara), wasabi, cottonweed cypress (mitsumenbana), waremoko (jimyu) and the like.

Other seawater, for example, seawater salt, dried seawater, inorganic salt obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango) , For example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide , Calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic,
Wed), Shotoku stone etc.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaa nori, Susiaonori, Hiraaonori, Bowaonori, Hosoedaoonori), Anaaousa algae [Aosaa] Kinds: Kombu (Macambu, Lysicum, Hosomekombu, Mitsuikonbu), Wakame, Hirome, Aookame, Giant Kelp (Macrocystis pilifera, Macrocystis integrifolia, Neocitys luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Klebanoumi prickly pear, Akaba prickly pear, Kona prickly pear, Okina prickly pear, Usuki prickly pear, Echinoki prickly pear], seaweed [red algae: red alga: Hijirimen, maxa (Tengusa), hirakusa, oniku , Obakusa, Kataobakusa,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Juniperus giraffe, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetsunomata, Hirakotonota, Kotoji, Kotojita
Jareus Banori, Kagius Banori, Siusius Banori, Hyus Banori, Akamomininori] and the like.

Other algae such as green algae (Chlamydomonas spp .: Chlamydomonas spp., Akayukimo, Dunaliella spp .: Dunaliella spp., Chlorococcus spp .: Chlorococcus, Kwanomimo spp .: Kwanomimo spp., Volvox spp. Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidoro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: magnolia, asamidorigusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: agatamagu, iwata: husaiwata, suricogita, heraiwata, crocodile, crocus Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizodzumo, genus Genus, genus Colecaetus, genus Ceratophylla, genus Ceratopogon: Rubiaceae, Rana genus: Rana genus, Hirano-Hana, Rana genus, Rhizophora, Genus genus, etc.

Blue-green algae (Genus Lynch: L., L., L., L .: L., mushrooms,
(Hassay, genus genus, genus racenmo (spirulina): spirulina, genus trichodesmium (aiakacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp .: Namiami spp., Isogrape spp .: Isogrape,
Isogawara genus: Isogawara, Kuroshira genus: Gunsenkurogashira, Kashirazaki genus: Kashirazaki, Muchimo genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa genus: Amidigusa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common genus: Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawa mozuku, genus nov. Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Species of Coleoptera, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Kayamono, Genus Genus: Genus Ligustrum, Genus Tsurumo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Missiscoma: Genus Missiscoma, Atsubumikonga, Kossula , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombe genus: Kikuishicomb, Nejirekombu: Nekorecome, Kuroshimeo : Nekoshikonbu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yabanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-mokum, Obanoki-mimoku, Genus genus:
Bulumimo, genus cynomolgus: cynomolgus etc.).

Red algae (Russia genus: Ushikenori, Funorinoushike, Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acacia sylvestris, Ochryllidae, Larvae genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: Rattle, Hiragaragara, Hirohatamataki: Hirohatamaitadaki, Tamaitadaki: Tamaitadaki, Kakenokenori: Kaginori, Kakenenori, Tengusa: Himetengusa, Hitenugusa, Hitenugusaobana, Hitennakuobusa, Hitenuganugusa, Hitenugusaugou Genus: hibiroudo, himebiroudo,
Genus Isomomedogi: Genus Isomomedo, Genus genus: Genus genus, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekigusa: Nisekaregusa, Okutsubara: Oba Otsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattles, genus cynosporium: shiogosagoromo, genus iwanokawa: esukiiwanokagawa, genus kainokawa: genus kainokawa: kaninote, genus coral: coral genus, coral mosquito, mosquito mosquito , Tambanori, slippery,
Fudarak, Malbahudarak, Isonohana: Nuracusa,
Black skull, scorpion scorpion, squash genus: squamous ibis, scorpion genus: scorpion genus: scorpion sculpture: chabo kintoki, squash, matsunori, komenori, tosakamatsu, tsutsumatsu, fushikintoki, tsunomukade, swordfish, swordfish, saccharomyx, skullfish Nagaobabane: Nagaobane,
Genus Funori: Hanafunori, Fukuronori, Makunori, Karenikusa: Kalexigusa, Tosokamodoki: Hosobanotosakamaki, Hirohanokasudo, Yatsudegatatosakamoki, Kurosakamoka, Nezashinotosakanodoka, Yukasinosa Modoki,
Genus genus: genus genus: genus: kinuhada, genus ezotosaka: genus ezotosaka, genus tsukasanori: genus california, giant porpoise, genus hagano genus: genus honoo, genus genus: genus lycopodium, genus genus Susqueke Beni, Okamuragusa:
Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiinobalon, Kijunio genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurusilamo, Shiramo, Ogogonori, Mizookonori, Kabanori, Beniogonori, Fucresinori, Murasakikabanori, Shinkaikabanori, Togekabanori, Kataogonori, Ryukyuogonori, Ryokyuogonori, Ryukyuogonori, Ryukyuogonori, Ryokyuogonori , Genus Asteraceae: Hachijoenagasomodo, Fushikenori genus: Fushikenori, Namiiwatake genus: Namiiwatake, scorpion genus: Scorpion genus, Occidental genus: Okutsunori, Saimi genus: Itanigusa, Simi, Harigane, Lotus japonicus:
Lotus lotus, genus genus: squid
Nobonori, Black Snapper genus: Black Snapper, Red Snapper genus: Red Snapper, Hishbukuro genus: Hishbukuro, Madaragusa genus: Togemadara, Echimadara, Taoyagusa genus: Taoyagousou, Hanasakura, Saga-no-nagusa , Hirata goat: Hirata goat, Darus: Darus, Masagoshibari, Anadals, Uebagsa: Uebagsa, Benifukuronori: Benifukuronori, Fushitsuna: Fushitsunagi, Himefushinagi,
Species of the genus Hirohafushi and Snapdragons: Snapdragons, Snapdragons, Species: igis, keiigis, crickets, nigiri, amixa, egonori:
Egonori, Futogigis, Saeda: Saeda, Chrysanthemum: Chrysanthemum, Konohanori: Habtaenori, Konohanori, Suzushironori, Usveni: Usveni, Hasuginu: Hasuginu, Nagakonohori: Nagakosugino, Genus suginanu, Hana Yaleusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki genus: Usubanorimodoki, Ayanishiki: Ayanishiki, Ayaginu:
Ayaginu, Dassia Genus: Enashidazia, Shimadada Genus: Isohagi, Shimadadaji, Dazimodoki Genus: Dazimomodoki, Itogusa Genus: Moletogusa, Futonigusa, Macri Genus: Macri, Salixinus Genus: Hanayanagi, Yuna, Sanagiginori, Mosanyugina, Genus : Kurozozo, Kobusoso, Hanezozo, Sozonohana, Hanegusa: Hanegusa, Kanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Himegoke: Himegoke, Kurohimegoke, Hyodosigus, Genus genus Suginasugusa, Isobasho Genus: Isobasho, Fujimatsumo Genus: Fujimatsumo, Sawtooth Lilium: Hatakesakinokokihiba, Kawamozuku: Kawamozuku, A Kawamozuku, Himekawamozuku,
Ideyugogome: Ideyugogome, Okitimozuku: Okitimozuku, Itogusa, Chinorimo: Chinorimo, Chisginori: Chisinori, etc.).

Axle algae (Characta spp., Bracium spp.,
Hossiturimo: Hossiturimo, Lichnotumnus, Flasks: Himefuramo, Chaboflasmo, Tripella etc., and yellow algae (Hikarimo: Hikarimo etc.).

Examples of the material derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, a pig or cow extract of the stomach, duodenum or intestine or a decomposition product thereof, and a pig or cow spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Milk, casein and its degradation products or their derivatives, skim milk powder and its degradation products or their derivatives, lactoferrin or its degradation products, chicken egg components, fish meat degradation products, nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid) and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran (red bran, white bran) ferment extract, euglena extract, raw milk or skim milk powder Lactic acid fermentation products, trehalose or derivatives thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonic acid salt, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid Derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate,
Octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, mono-2-diparamethoxycinnamate
Glyceryl ethylhexanoate, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate,
Methyl salicylate), anthranilic acid derivatives (methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin Guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and the like.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate ester magnesium salt, vitamin C glucoside or the like), hydroquinone or a derivative thereof (hydroquinone benzyl ether or the like), kojic acid or a derivative thereof, vitamin E or Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, corn, waremokou, clara, mugwort, Honeysuckle, yellowfin, yellowtail, matsuhodo, adlay, dragonfly, hops, hawthorn, eucalyptus, achillea millefolium, altea, keihi, mankeishi,
Hamamelis, yamaguwa, life-prolonged grass, bellflower, toshishi, spouse, sun-dried, mako, senkyu, dokkatsu, psycho, bohu, hamabou, ogon, peony, peony,
Gennoshoko, kakkon, licorice, five times child, aloe, ginger, safflower, green tea, black tea, asenyaku), etc.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, hydrogen peroxide solution, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether)
etc.

(21) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odorikosou, Assemblage, Touki, Tokinsenka, Amacha, Hypericum, Cucumber, Periwinkle,
Rosemary, parsley) etc.

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) etc.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, propolis, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emisoso, Birch, Salvia, Rosemary, Minami Tenji, Agetsu, Ginkgo, Green Tea) and the like.

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract extract, propolis and the like.

(27) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octyl phenyl ether, etc.), amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, potassium lauroyl sarcosine, etc.),
Cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine,
Brilliant Green, Malachite Green, Crystal Violet, Jarmal, Photosensitizer No. 101, Photosensitizer No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin, propolis, etc. .

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Bacillus natto extract, Loofah water, Birch (birch) or Akamatsu sap

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, salvia, elderberry, bollige, buttonpi), seaweed extracts and the like.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Stimulants Pepper tincture, vanylamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid or a derivative thereof, allantoin, placental extract, biotin, glyceride pentadecanoate and the like.

(35) Antiseborrheic agent pyridoxine or a derivative thereof, sulfur, vitamin B6
etc.

(36) Keratolytic agents Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, Coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Fengqiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batuuri essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Essential oils, vegetable flavors such as Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / Colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, fresheners, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and antiallergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral agents, deodorants / deodorants, bittering agents, seasonings, enzymes, and the like can be used, and when used in combination therewith, various additive and synergistic effects can be expected.

The hydrogen peroxide scavenger, anti-inflammatory agent and cosmetic composition of the present invention may be in any form such as capsules, powders, granules, solids, liquids, gels, air bubbles, emulsions, creams, ointments and sheets. It can be used by blending it into cosmetics and bath preparations.

Specifically, for example, external preparations such as medicinal preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, depilatory agents, depilatory agents, aftershave lotions , Pre-show lotion, shaving cream, shampoo, rinse,
Hair treatments, hair styling products, perm agents, hair tonics, hair dyes, hair growth and hair growth products, etc., makeup cosmetics such as foundation, lipstick, blusher, eyeshadow, eyeliner, mascara, perfumes, bath agents , Other, toothpaste, mouth freshener / gargle, deodorant,
It can be applied to various products such as deodorant, sanitary cotton, wet tissue, and can be used for general food and drink.

The method of adding the hydrogen peroxide scavenger, the anti-inflammatory agent and the cosmetic composition of the present invention to the cosmetic composition may be added in advance or during the production, and the workability is considered. May be appropriately selected.

[0078]

EXAMPLES The present invention will now be described with reference to Production Examples, Test Examples and Formulation Examples, but the present invention is not limited thereto.

(Culture Example 1) 5 g of yeast extract, 5 g of peptone, 5 g of glucose, 1 g of K ₂ HPO _4, 1 g of MgCl per 0.9 L of purified water
_2. Dissolve 0.2 g of H ₂ O and add 5 g of glycine to a sterilized medium.
2.5 g of cysteine and 5 g of glutamic acid were dissolved, and the pH was adjusted to 5.0 to 7.0 with a pH adjuster to make the whole 1 L. After 150 mL of the medium thus prepared was extracted into a flask sterilized by dry heat, yeast (Saccharomyces cerevisiae) was added to the flask.
A platinum loop was inoculated and shake-cultured at 28 to 35 ° C for 4 days, and then the culture was centrifuged to collect the cells.

(Production Example 1) A 0.5% glycerin solution was added to a dry powder of yeast containing high glutathione, followed by heat extraction.
Leave at room temperature overnight. This liquid was filtered, and a 1,3-butylene glycol solution (25 kg) was added to the obtained filtrate (75 kg), followed by filtration.

(Production Example 2) Glutathione-rich yeast (0.
1 to 1.0 kg) in purified water (10 L), and
Let them self-digest for about an hour. Then, this was freeze-dried or concentrated under reduced pressure, and a 30% 1,3-butylene glycol solution (10
After L) was added and sufficiently extracted with stirring, the mixture was centrifuged and filtered.

(Production Example 3) Glutathione-rich yeast (0.
1 to 1.0 kg) in purified water (10 L), and further protease (eg, actinase A or B manufactured by Kaken Pharmaceutical Co., or protease Amano P, 3-50 manufactured by Amano Pharmaceutical Co., Ltd.)
And reacted at around 37 ° C for 24 hours. Next, after inactivating the enzyme, propylene glycol was added to a concentration of 30%, followed by centrifugation and filtration.

(Production Example 4) Glutathione-rich yeast (0.
1-1.0 kg) is suspended in 3% hydrochloric acid (10 L) at a temperature of 40-60.
Hydrolysis at 0 ° C. for 3-8 hours with occasional stirring. After the decomposition, the mixture was neutralized with an alkali, centrifuged, and filtered.

(Test 1) Hydrogen peroxide scavenging test In this test, a hydrogen peroxide scavenging test using a peroxidase-fixed electrode to evaluate the hydrogen peroxide scavenging effect (see Ann. Clin. Biochem. 35 (1998) ) 739-744,
Using J. Electroanal. Chem. 478 (1999) 33-39), the active oxygen scavenging action was evaluated by mutation of the current.

(Test Method) a. Sample The yeast extract obtained in Production Example 1 was adjusted to a solid content of 0.1% by weight and used as a sample. As a control, a normal yeast extract was used. b. Peroxidase fixed electrode Ferrocene, liquid paraben, and graphite powder
After mixing at a weight ratio of 1: 4: 10 and sufficiently kneading, the mixture was filled in a concave portion of a carbon paste electrode (manufactured by BAS, No. 11-2210, inner diameter 3 mm), and the surface was rubbed with paraffin paper to make it smooth.
After 2 μL of a 10 mg / mL peroxidase solution was applied to the surface and water was evaporated, a 6 mm diameter circular dialysis membrane (product of Spectrum) with a molecular weight cut off of 100 was coated, and it was covered with a nylon net. The net was fixed with a laboratory film. c. Constant-potential current measurement Put 1 mL of a base solution (10 μM aqueous hydrogen peroxide solution) into an H-type cell, stir with a stirrer, and measure the decrease in hydrogen peroxide when a certain amount of sample (1 mL) is added. The constant potential current was measured using a polarographic analyzer P1100 and a Yt recorder EPR151-A manufactured by Toa Denpa Co., Ltd. using an electrode system with a saturated calomel electrode (SCE) as a counter electrode. Measurement is 37 ° C
The results are shown in FIGS. 1 and 2.

(Results) As shown in FIGS. 1 and 2, the current value of the product of the present invention of Production Examples 1 to 4 was lower than that of the conventional yeast extract as the comparative example, which showed a gradual decrease in the current value. After reaching the peak, it showed a sharp decline. Furthermore, the yeast extract of Production Example 1 exhibited a remarkable lowering effect. Therefore, it was confirmed that the yeast extract of the present invention had a stronger hydrogen peroxide eliminating action than the normal yeast extract. Further, among the products of the present invention, a higher evaluation was obtained for a sample having a high glutathione content.

(Test 2) Carrageenan footpad edema suppression test (anti-inflammatory effect test) It is known that carrageenan-induced edema changes in chemical mediators involved in inflammation that occurs after administration. For example, within 1 hour of administration, serotonin and histamine are 1-2
It is said that kinins are involved in the time and the plastaglandin system is involved in the second and subsequent hours, and the time from the start of the test to the time when the inhibitory effect appears is used to estimate which chemical mediator is being suppressed. be able to. Therefore, a substance that suppresses edema of the carrageenan footpad can be expected to have an anti-inflammatory response. This test was performed using mouse footpad edema test (Cosmetic Journal, 2
3,267 (1990)), the extracts obtained in Production Examples 1 to 4 were examined.

(Test Method) a. After evaporating the solvent under reduced pressure, the sample extract was redissolved in purified water to a solid content concentration of 10% by weight and used for the test. or,
When used in combination, the respective solid content concentrations were adjusted to 10% by weight. As a control, a common yeast extract of the same concentration (extracted according to Production Example 2) was used. b. Measurement of edema swelling rate The thickness of the right hind footpad of a 6-week-old ICR female mouse was measured in advance with a dial thickness gauge, and the carrageenin was administered twice, one hour before administration, and the test drug 25 mg was added to the right. It was applied to the hind limbs so that it could be carefully rubbed with fingers 30 times. One hour later,
25 μL of physiological saline containing 2% λ-carrageenin under the right hind footpad
The thickness of the footpad was measured every 6 hours thereafter for 6 hours, and the difference from the thickness of the footpad before λ-carrageenin administration was determined. The suppression rate was determined, and the results are shown in FIG. In the test, 7 to 8 mice were used.

(Results) As shown in FIG. 3, the product of the present invention showed a carrageenan footpad edema inhibitory effect significantly more than that of a general yeast extract as a control. Therefore, the product of the present invention can be expected to have an anti-inflammatory effect.

(Test 3) Safety test (1) Skin primary irritation test The product of the present invention obtained in Production Examples 1 to 4 had a solid content of about 1.0.
%, And this was applied to each animal at 0.03 mL per 2 × 2 cm ² of the skin of a Hartley-type guinea pig (female, 3 per group, body weight of about 340 g) whose back was shaved. Judgment was carried out 24, 48, and 72 hours after application using the primary irritation scoring method with erythema and edema as indices. As a result, in all the animals, no erythema and edema were observed, and the judgment was negative.

(Test 4) Safety Test (2) Skin Cumulative Irritation Test The product of the present invention obtained in Production Examples 1 to 4 had a solid content of about 2.0.
% And comprising as prepared in purified water, which flank shaved (2 × 2 cm ²⁾ were Hartley guinea pigs (female, 3 mice per group, body weight 320g longitudinal) Skin 2 × 2 cm ² per one day 1
0.03 mL was applied to each animal five times a week. The application was performed over a period of two weeks, and the shaving was performed as appropriate. The determination was made on the day following each application day and on the day after the last application day, using primary irritancy scoring as an index of erythema and edema. As a result, in all animals, no erythema and edema were observed for 2 weeks after application, and the animals were determined to be negative.

(Test 5) Safety Test (3) Single Dose Toxicity Test The product of the present invention obtained in Production Examples 1 to 4 has a solid content of 12.5%.
Adjust with purified water to a concentration of 16mL / kg per animal
The dose was orally administered to a ddy mouse (male and female, 5 mice / group, 5 weeks old) which had been fasted for 16 hours before the test by oral administration, and the appearance and degree of toxic symptoms were observed with time. .
As a result, no abnormality was observed in all the mice for 14 days, and no abnormalities were found in the dissection results. Therefore, LD ₅₀ is 2,
It was 000 mg / kg or more, so it was determined to be a safe substance.

(Formulation Examples) Production of Various Cosmetic Compositions In accordance with the above evaluation results, the formulation examples are shown below. Each formulation example may be one produced by a conventional method in the production of each product. showed that. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7.1,3-butylene glycol 1.0 8. Glycerin 2.0 9. Yeast extract of Production Example 1 5.0 10. Preservative (paraoxybenzoate) qs 11. Purified water 100

(Formulation Example 2) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Glyceryl paraaminobenzoate 1.0 4. Ethyl urocanate 1.0 5. Magnesium salt of vitamin C phosphate ester 1.0 6. Cetyl alcohol 1.5 7. Glycerin monostearate 2.0 8. Polyoxyethylene (20) cetyl ether 3.0 9. Polyoxyethylene (20) soorbitan monooleate 2.0 10.1,3-butylene glycol 1.0 11. Glycerin 2.0 12. Yeast extract of Production Example 3 3.0 13. Preservative (p-hydroxybenzoate) Appropriate amount 14. Purified water 100 residue

(Formulation Example 3) Cosmetic oil weight% 1. Liquid paraffin 30.0 2. Squalane 20.0 3. Olive oil 20.0 4. Isopropyl palmitate 10.0 5. Crepefruit hot water extract 1.0 6. Yeast extract of Production Example 2 3.0 7. Olive oil 1.0 8. Shea butter 1.0 9. Butyl hydroxyanisole 0.1 10. Vitamin E derivative 0.1 11. Preservative (benzalkonium chloride) proper amount 12. Perfume (musk water) proper amount 13. Purified water 100 residue

(Formulation Example 4) Flexible lotion weight% 1. Glycerin 5.0 2.1,3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. Yeast of Production Example 1 Extract 1.0 6. Grapefruit 50% ethanol extract 1.0 7. Kiwi 50% ethanol extract 1.0 8. Appropriate amount of antibacterial and preservative (lactoferrin solution) 9. Appropriate amount of fragrance (Western thin water) 10. Purified water 100 residue

(Formulation Example 5) Astringent lotion weight% 1. Sorbit 2.0 2. POE (20) oleyl alcohol 1.0 3. Ethanol 15.0 4. Yeast extract of Production Example 2.0 2.0 5. Gentian 50% ethanol extract 1.0 6 Sage 50% ethanol extract 1.0 7. Allantoin 0.2 8. Astringent (zinc sulfocarbonate) 0.2 9. Antibacterial and preservative (thiamine lauryl sulfate) qs 10. perfume (lavender water) qs 11. Purified water 100 Residue

(Formulation Example 6) Two-layer type lotion (liquid to liquid type)% by weight 1. Squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. Sorbit 1.0 4. Ethanol 15.0 5. Yeast extraction of Production Example 2 Stuff 2.0 6. mallow 30% ethanol extract 2.0 7. peonies 50% ethanol extract 1.0 8. peanut 50% ethanol extract 1.0 9. ascorbic acid 0.2 10. astringent (calamine) 0.1 11. preservative (phenoxyethanol) Appropriate amount 12.Fragrance (lemon water) Appropriate amount 13.Purified water 100 residue

(Formulation Example 7) Carmine lotion (liquid to solid)% by weight 1. Glycerin 3.0 2.1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Yeast extract of Production Example 3 2.0 8. Carrot 50% propylene glycol extract 1.0 9. Placenta extract 1.0 10. Camphor 0.2 11. Phenol 0.03 12. Preservative (photosensitive element 201 & 401) qs 13. Perfume (bodhi tree water) ) Reasonable amount 14. Residue to make purified water 100

(Formulation Example 8) Pack weight% 1. Polyvinyl alcohol 15.0 2. Sodium carboxymethyl cellulose 5.0 3. Propylene glycol 3.0 4. Ethanol 10.0 5. Yeast extract of Preparation Example 1.0 6. Yeast extract of Preparation Example 2 1.0 7. Thyme 50% ethanol extract 1.0 8. Melissa 50% ethanol extract 1.0 9. Preservative (paraoxybenzoate) qs 10. qflavour (tokisui) qs 11. Purified water 100 balance

(Formulation Example 9) Peel-off pack weight% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Preparation example 3 Yeast extract 1.0 8. Safflower 50% ethanol extract 1.0 9. Yuzu 50% ethanol extract 1.0 10. Preservative (paraoxybenzoate) qs 11. Fragrance (cypress water) qs 12. Purified water 100

(Formulation Example 10) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 5.0 5. Chamomile oil 5.0 6. Palm oil 5.0 7. Borax 0.5 8. Production example 4 Yeast extract 1.0 9. liquid shea butter 1.0 10. preservative (acrinol) 0.1 11. perfume (sage water) appropriate amount 12. purified water 100

(Formulation Example 11) Emollient cream% by weight 1. Stearic acid 2.0 2. Stearyl alcohol 7.0 3. Reduced lanolin 2.0 4. Squalane 5.0 5. Octyldodecanol 6.0 6. Polyoxyethylene cetyl ether (25E.O.) 3.0 7. Lipophilic glyceryl monostearate 2.0 8. Propylene glycol 5.0 9. Yeast extract of Production Example 1 3.0 10. Rosemary 50% 1,3-butylene glycol extract 2.0 11. Preservative (paraben) qs 12. Perfume (cinch water) Appropriate amount 13. Residual water with 100 purified water

(Formulation Example 12) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Yeast extraction Product 2.0 (Ethanol: 1,3-butylene glycol = 1: 4 extraction solvent) 8. Loofah 45% 1,3-butylene glycol extract 2.0 11. Dipotassium glycyrrhetinate 1.0 12. Preservative (salicylic acid) 0.1 13. Fragrance (Sage water) Appropriate amount 14.Residual water to be 100

(Formulation Example 13) Face Wash Cream% by Weight 1. Stearic acid 10.0 2. Palmitic acid 10.0 3. Myristic acid 12.0 4. Lauric acid 4.0 5. Oleyl alcohol 1.5 6. Lanolin derivative (EO adduct) 1.0 7. Glycerin 18.0 8. Potassium hydroxide 6.0 9. Yeast extract of Production Example 3 5.0 10. Royal jelly extract 1.0 (ethanol: 1,3-butylene glycol = 1: 1) 11. Glycyrrhetinic acid 1.0 12. Preservative (salicylic acid) 0.1 13.Perfume (Tokisui) Appropriate amount 14.Residual water to be 100

(Formulation Example 14) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Whey 1.5 5. Yeast extract of Production Example 1 0.5 6. Amadir 70% 1 , 3-butylene glycol extract 0.5 7. Polyethylene powder 0.5 8. Hydroxypropyl chitosan solution 0.5 9. Amino acids (glycine, glutamine, etc.) 0.5 10. Preservatives (undecylenic acid, phenol) qs 11. pH adjuster qs 12. Fragrance (Lavender water) Appropriate amount 13. Residual water with 100 purified water

(Formulation Example 15) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Polyethyl acrylate 1.0 9.1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 1.0 12. Titanium dioxide 5.0 13. Yeast extract of Production Example 2 0.5 14 Honeysuckle 50% dipropylene glycol extract 0.5 15. Soybean ash 50% dipropylene glycol extract 0.5 16. Sicon 50% dipropylene glycol extract 0.5 17. Preservative (paraben) qs 18. Fragrance (apple water) qs 19. Residue with 100 purified water

(Formulation Example 16) Sunscreen cosmetics (oil type)% by weight 1. Liquid paraffin 70.0 2. Ethyl paraaminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Yeast extraction of Production Example 3 Product 1.0 6. Aloe 50% 1,3-butylene glycol extract 0.5 7. Sakinoshita 30% 1,3-butylene glycol extract 0.5 8. Yoquinin 50% 1,3-butylene glycol extract 0.5 9. Bodaiju 45% 1 , 3-butylene glycol extract 0.5 10. Suitable amount of antioxidant (butylhydroxytoluene) 11. Suitable amount of fragrance (Bodyju water)

(Formulation Example 17) Cream foundation (O / W type) wt% 1. stearic acid 4.0 2. glyceryl monostearate 3.0 3. cetanol 1.5 4. isopropyl myristate 7.0 5. liquid paraffin 10.0 6. salami beeswax 3.0 7. Triethanolamine 1.5 8. Glycerin 3.0 9. Bentonite 1.0 10. Titanium oxide 8.0 11.2,4-dihydroxybenzophenone 8.0 12. Kaolin 3.0 13. Talc 3.0 14. Color pigment (iron iron oxide) 1.0 15. Production Example 1 Dry powder of yeast extract 0.2 16. Preservative (paraben) suitable amount 17. Fragrance (rose water) proper amount 18. Purified water 100 residue

(Formulation Example 18) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Yeast extract of Production Example 2 2.0 7. Hydroxypropyl chitosan solution 2.0 8. Mucopolysaccharide solution 1.0 9. Antibacterial and preservative (photosensitive element 101) qs 10. pH adjuster qs 11. Fragrance (sandalwood water) Appropriate amount 12.Purified water 100 residue

(Formulation Example 19) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Yeast extract of Production Example 3 1.0 6. Novara 50% 1,2-butylene glycol extract 1.0 7. Futandandelion 50% 1,3-butylene glycol extract 1.0 8. Hydroxyethyl chitosan solution 1.0 9. Trehalose solution 1.0 10. Hydrolyzed silk solution 1.0 11. Antibacterial Preservative (benzalkonium chloride) qs 12. flavor (lavender water) qs 13. purified water 100

(Formulation Example 20) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Ethylene glycol 3.0 3. Yeast of Production Example 4 Extract 1.0 7. Maronier 50% ethylene glycol extract 1.0 8. Trehalose solution 1.0 9. Hydrolyzed keratin solution 1.0 10. Appropriate amount of antibacterial and preservative (benzalkonium chloride) 11. Appropriate amount of fragrance (chamomile water) 12. Residue with 100 purified water

(Formulation Example 21) Hair liquid% by weight 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Yeast extract of Production Example 1 2.0 6 Apricot fruit or leaf 30% ethanol extract 1.0 7. Melissa hot water extract 1.0 8. Panax ginseng 50% ethanol extract 1.0 9. Carpronium chloride 0.3 10. Hinokitiol 0.5 11. Keratin decomposition solution 1.0 12. Antibacterial and preservative ( Paraben, benzalkonium chloride) Appropriate amount 13.Fragrance (Senkyu water) Appropriate amount

(Formulation Example 22) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Trimethylene glycol 5.0 6. Production Example 4 Yeast extract 1.0 7. Parsley 50% 1,3-butylene glycol extract 0.5 8. Loquat leaf 30% ethanol extract 1.0 9. Azulene 1.0 10. Antibacterial and preservative (paraben, benzalkonium chloride) Appropriate amount 11. Perfume (Touki water) Appropriate amount 12. Residual water with 100 purified water

(Formulation Example 23) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Yeast extract of Production Example 5.0 5. Peony skin 50% Ethanol extract 3.0 6. Yuzu leaf or fruit 50% ethanol extract 3.0 7. Preservative (paraben) qs 8. Purified water 100 residue

(Formulation Example 24) Hair tonic weight% 1. l-menthol 0.2 2. Ethanol 32.5 3. Polyoxyethylene hydrogenated castor oil 0.5 4. Propylene glycol 5.0 5. Diphenhydramine hydrochloride 0.3 6. Yeast extract of Production Example 3 1.0 7. Dutch mustard 50% ethanol extract 1.0 8. Ougon 50% ethanol extraction solution 1.0 9. Carboxymethyl chitin solution 1.0 10. Vitamin E derivative 0.5 11. Antibacterial and preservative (paraben) 0.1 12. Fragrance (sage water) Appropriate amount 13.Remaining amount of purified water 100

(Formulation Example 25) Hair restorer / hair restorer% by weight 1. Ethanol 60.0 2. Yeast extract of Production Example 4 5.0 3. Ginseng 50% propylene glycol extract 2.0 4. Artemisia 50% 1,3-butylene glycol extraction 1.0 5. 50% 1,3 butylene glycol extract of wicker nut 1.0 6. Vitamin E derivative 0.5 7. Pepper tincture 0.5 8. Resorcin 0.5 9. Dipotassium glycyrrhizinate 0.5 10. Carboxymethyl chitin solution 0.5 11. Bovine hematin solution 0.5 12. Mucin solution 0.5 13. Amino acids (glycine, glutamine, etc.) 0.5 14. Antibacterial and preservative (paraben) 0.1 15. Perfume (rosemary water) qs 16. Purified water 100

(Formulation Example 26) Hair restorer / hair restorer weight% 1. Ethanol 60.0 2. Yeast extract of Production Example 1.0 3. 3. 60% ethanol extract of assembly 1.0 4. Cantharistincini 0.5 5. Pantothenic acid derivative 0.5 6. Glycyrrhizic acid 0.5 7. Resorcinol 0.5 8. Salicylic acid 0.5 9. Hydroxyethyl chitosan solution 0.5 10. Elastin hydrolyzate 0.5 11. Antibacterial and preservative (paraben) 0.1 12. Flavor (lavender water) qs 13. Purified water 100 Residue

(Formulation Example 27) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 2.0 4. Dry powder of yeast extract of Production Example 1 5. Drying with eucalyptus hot water extraction Powder 5.0 6. Dye (Gunjo green) Appropriate amount

(Formulation Example 28) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 4.0 4. Dry powder of yeast extract of Production Example 2.0 5. Yeast of Production Example 3 Extract dry powder 1.0 6. Satsuma mandarin 50% ethanol extracted dry powder 1.0 7. Peach leaf 70% ethanol extracted dry powder 1.0 8. Iris 70% ethanol extracted dry powder 1.0 9. Apple juice powder 1.0 10. Pigment (Gunjo, Chromium hydroxide) Appropriate amount 11. Fragrance (grape water) Appropriate amount

(Formulation Example 29) Coating protective agent A gauze or liniment cloth is impregnated with a formulation solution prepared by mixing an appropriate amount of the yeast extract of Example 1, an antibiotic, an anti-inflammation, etc., and attached to the wound. Alternatively, the yeast extract may be directly applied locally and covered with gauze or the like.

(Test 6) Use effect test The effect when the cosmetic composition of the present invention was actually used was examined. Use test is a panel of 10 people aged 30 to 60 who are worried about dry skin, glossy and firm skin, and apply the appropriate amount of the emulsion of Prescription Example 1 to the face twice a day, twice a day, in the morning and at night after washing the face. This was done by coating over.
As a control, a normal yeast extract similarly formulated in place of the present invention was used. Furthermore, instead of the present invention, glutathione similarly formulated was used. The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numbers in the table represent the number of persons. No one complained of abnormal skin during the use period.

"Effect of improving skin feel" Efficacy: Dry skin / roughness, gloss / tension of skin increased, and skin was improved. Slightly effective: Dry skin / roughness, skin gloss / tension increased slightly, and skin was improved. Ineffective: No change from before use.

[0125]

(Test Results) As shown in Table 1, it was confirmed that the emulsion containing the yeast extract of the present invention significantly improved dry skin and gave gloss and tension to the skin. Glutathione used as a control did not give a good evaluation. This confirmed that the yeast extract containing glutathione at a high content by culture had an excellent skin feel improving effect.

[0127]

According to the present invention, the genus Saccharomyces (Sacch
aromyces) A yeast extract having a glutathione content of 0.0001% by weight or more as a solid content, which is metabolized by culturing yeast, has an excellent hydrogen peroxide scavenging action and an anti-inflammatory action as compared with a normal yeast extract. In addition, it has been found that the usefulness and usability for use in cosmetics are remarkably improved. In addition, a cosmetic composition containing these compounds can be expected to improve rough and dry skin, give the skin a luster and firmness, and prevent aging caused by active oxygen.

[Brief description of the drawings]

FIG. 1 is a diagram showing the hydrogen peroxide scavenging effect of the yeast extract of Production Example 1 (glutathione content 0.0035% by weight) and the yeast extract of Production Example 2 (glutathione content 0.0029% by weight), which are products of the present invention. is there. As a comparative example, a normal yeast extract (glutathione content 0.0010% by weight) was used. In the figure, each sample was added at the time point indicated by an arrow (↓).

FIG. 2 is a graph showing the hydrogen peroxide scavenging effect of the yeast extract of Production Example 3 (glutathione content 0.0031% by weight) and the yeast extract of Production Example 4 (glutathione content 0.0024% by weight), which are the products of the present invention. is there. As a comparative example, a normal yeast extract (glutathione content 0.0010% by weight) was used. In the figure, each sample was added at the time point indicated by an arrow (↓).

FIG. 3 shows a carrageenan footpad edema suppression test (anti-inflammatory effect test) of the product of the present invention.

────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] February 22, 2001 (2001.2.2)
2)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0019

[Correction method] Change

[Correction contents]

The hydrogen peroxide scavenger, anti-inflammatory agent, and cosmetic composition of the present invention may further comprise, if necessary, the following components within the range that does not impair the effects of the present invention. And additives can be arbitrarily selected and used in combination, and the content in the preparation is not particularly defined, but usually,
0.0001 to 50% is preferred.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (reference) A61K 7/00 A61K 7/00 U 7/02 7/02 Z 7/06 7/06 7/075 7 / 075 7/08 7/08 7/11 7/11 7/42 7/42 7/50 7/50 A61P 29/00 A61P 29/00 39/06 39/06 C12N 1/16 C12N 1/16 A // C12P 21/02 C12P 21/02 G (C12N 1/16 (C12N 1/16 C12R 1:85) C12R 1:85) (C12P 21/02 (C12P 21/02 C C12R 1:85) C12R 1:85) (72) Inventor Norihiro Banno 318 Ichimaru Falcos Co., Ltd. at 318 Asagi, Masamasa-cho, Motosu-gun, Gifu Prefecture Term (reference) 4B064 AG01 CA06 CE08 DA01 4B065 AA79X BD10 BD16 CA24 4C083 AA031 AA032 AA0 72 AA082 AA112 AA122 AB032 AB152 AB212 AB232 AB242 AB312 AB352 AB432 AB442 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC342 AC352 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC552 AC582 AC642 AC682 AC692 AC782 AC792 AC852 AC902 AD022 AD042 AD532 AD552 AD642 AD662 CC03 CC04 CC05 CC06 CC07 CC12 CC19 CC23 CC25 CC32 CC37 CC38 CC39 DD16 DD22 DD23 DD30 DD31 DD33 EE01 EE10 EE12 EE17 EE22 4C087 AA01 AA02 BC12 CA11 MA63 NA14 ZB11 ZC80

Claims (3)

[Claims] 1. A hydrogen peroxide scavenger comprising a yeast extract having a glutathione content of 0.0001% by weight or more as a solid content by culturing Saccharomyces yeast. 2. An anti-inflammatory agent comprising a yeast extract having a glutathione content of 0.0001% by weight or more as a solid content by culturing Saccharomyces yeast. 3. The hydrogen peroxide scavenger according to claim 1, and / or
A cosmetic composition comprising the anti-inflammatory agent according to claim 2.