KR101922637B1 - Cosmetic composition for skin whitening and improving skin wrinkle comprising horse oil and Rumex acetosella L. extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening or wrinkle improvement comprising a hemp and a swim swimming extract as an active ingredient and a cosmetic composition comprising a mixture of a hemp oil and a baby swim extract of the present invention as an active ingredient has excellent skin whitening and wrinkles Improvement effect. In addition, the purified tablet of the present invention, a swine swimming extract, and a mixture thereof are derived from natural materials, and do not cause cytotoxicity, and thus are highly utilized as a cosmetic composition.

Description

[0001] The present invention relates to a cosmetic composition for skin whitening and wrinkle improvement,

The present invention relates to a cosmetic composition for skin whitening or wrinkle improvement, which comprises an extract of Maesil and a swine swimming as an active ingredient.

The human skin consists of the epidermis, dermis, and connective tissue including the stratum corneum, and the stratum corneum is composed of a dead cell layer formed through the differentiation process of keratinocyte, the basal cell of the epidermis And is responsible for protecting the human body from the effects of the external environment. There are two major researches on the causes of skin aging. One is "internal aging", which is caused by changes in the function of cells, which are constitutive units of skin, with age, and the other is "external aging" Which can be divided into external environment such as ultraviolet rays, pollution, stress and the like.

Collagen, one of the important components of the skin dermal matrix, is a major substrate protein produced by the fibroblasts of the skin and is present in extracellular epilepsy. Its important functions are mechanical rigidity of the skin, resistance of connective tissues to tissue binding, And the like are known. Such collagen is reduced by external stimuli such as age and ultraviolet rays, which are known to be closely related to the wrinkling of the skin. The aging process reduces the synthesis of collagen itself and increases the activity of matrix metalloproteinases (MMPs) that degrade connective tissues including collagen, which promotes collagen degradation in skin tissue, resulting in decreased collagen content Is known to be. TIMP (Tissue Inhibitors of Matrix Metalloproteinase), which is an inhibitor of MMPs inhibition, is present. When the activity of MMPs and TIMP is appropriately controlled, physiological tissue remodeling and homeostasis are well maintained.

On the other hand, human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, skin color is also affected by environmental or physiological conditions such as sunlight, fatigue and stress. Melanin is synthesized in melanocytes in the epidermis of the skin. In the melanosome of melanocytes, an enzyme called tyrosinase acts on tyrosine, a type of amino acid, resulting in dopa (DOPA), dopaquinone ), And then it is made through a non-enzymatic oxidation reaction. When such a synthesis of melanin occurs excessively in the skin, it darkens skin tone, and causes spots and freckles. Thus, inhibiting the activity of tyrosinase in the skin to inhibit the synthesis of melanin pigment not only makes it possible to whiten the skin tone to brighten the skin tone, but also to reduce skin irritation caused by ultraviolet rays, hormones and hereditary causes, It can improve constipation.

Under these circumstances, the inventors of the present invention continued to develop a new natural substance having a whitening effect and a wrinkle-reducing effect while minimizing the side effects of human body. As a result, The present invention has been completed.

Korean Patent No. 10-1668154 Korean Patent No. 10-1700105

Accordingly, an object of the present invention is to provide a cosmetic composition for skin whitening or wrinkle improvement which comprises a mixture of horse oil and Rumex acetosella L. extract as an active ingredient.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for skin whitening or wrinkle improvement comprising a mixture of horse oil and Rumex acetosella L. extract as an active ingredient .

In one embodiment of the present invention, the hemp oil may be oil purified after dissolving horse meat.

In one embodiment of the present invention, the baby swimming extract comprises a baby swimming leaf, stem or outpost, which is composed of water, an anhydrous or a functional lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent or a mixed solvent thereof , And the like.

In one embodiment of the present invention, the hemp oil may be contained in an amount of 0.001 to 10 wt% based on the total weight of the composition, and the baby swimming extract may be contained in an amount of 0.001 to 10 wt% based on the total weight of the composition.

In an embodiment of the present invention, the cosmetic composition may be in the form of a lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, paste, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, , A wax, and a spray.

The cosmetic composition comprising the mixture of the hemp oil and the swine swimming extract of the present invention as an active ingredient can inhibit intracellular melanin production and has skin whitening effect as well as excellent collagen biosynthesis promotion effect and wrinkle improving effect. In addition, the purified tablet of the present invention, a swine swimming extract, and a mixture thereof are derived from natural materials and do not cause cytotoxicity, so that they can be effectively used as a cosmetic composition.

Brief Description of the Drawings Fig. 1 shows the result of measurement of cytotoxicity upon sample treatment in Murine melanoma cells (B16F10); Fig. 100 μM of α-MSH, 40 μM of Melasolv, 0.05% of purified hemp, 50 μg / mL of hemp swimming extract, and a mixture of purified hemp and hemp swimming extract.
FIG. 2 is a graph showing the results of measurement of the inhibitory effect on melanin formation by the sample treatment in mouse melanoma cells; 100 μM of α-MSH, 40 μM of Melasolv, 0.05% of purified hemp, 50 μg / mL of hemp swimming extract, and a mixture of purified hemp and hemp swimming extract.
FIG. 3 is a graph showing the results of measuring the increase of collagen production according to the sample treatment in human dermal fibroblast; (Control), 10ng / mL of transforming growth factor (TGF-β), 0.05% of purified hemp, 50μg / mL of hemp swimming extract,

The horse oil of the present invention is a fat ingredient extracted from the fatty tissue of horses. The Chinese medicine classical name of the medicine and the herbal ginseng root mean "hair" 'And' help the blood circulation ', and records of drug use in China in the 5th and 6th centuries have been recorded from ancient times in Asia to treat burns and atopic dermatitis Has been widely used. The main ingredients of maruyu are palmitoleic acid and ceramide. It is very similar to human sebaceous. It has high affinity with human skin and contains more than 60% of unsaturated fatty acid. It is a cosmetic raw material that can supply unsaturated fatty acid to skin. Be in the spotlight.

Rumex acetosella L. of the present invention is a perennial herbaceous plant of Polygonaceae and grows to a height of about 20 to 50 cm. The rootstock grows to the side and grows, the stem stands straight, the hair-like protuberance, the serpentine, the purple, the sour with leaves. Leaves from roots are clustered, petiole is long, spearlike, pointed end, and ear-like projections spread to the left and right. It is called Chrysophanic acid, Chrysophanol, Ascorbic acid, Rutin, Chrysophyllum, Chrysophyllum, Chrysophyllum, , Adenosine (Adenosine) and the like. In one room, the leaves and stems of baby swim are used as a medicinal material called small sow moth. It has anticancer effect and hemostatic effect on the blood which is caused by pulmonary tuberculosis. Transit from the private sector. ohm. dandruff. It is used as medicines for skin diseases. The outpouring extract is used for antiseptic, scurvy, hemostatic, diuretic, hemorrhoid, pulmonary tuberculosis, etc. Leaf extract is used for fever, headache, epilepsy, fracture and tumor as an analgesic agent (herbal composition and use, Dictionary Publishing Company, Chinese Medicine Dictionary, Book Publishing Charity, Chinese Medicines Editorial Committee, 1997).

The inventors of the present invention have conducted studies to develop such a hemp and swine swimming extract as a cosmetic composition and found that the hemp and swine swimming extracts exhibit a whitening effect as well as a whitening effect without cytotoxicity, Respectively.

Particularly, in the present invention, the effect of the present invention is remarkably enhanced when the mixture is used as compared with the case where the extract of Mayu, Swine swimming, and a mixture thereof is effective for inhibiting intracellular melanin formation and promoting collagen biosynthesis, (Examples 2 to 7). ≪ tb > < TABLE >

Accordingly, the present invention provides a cosmetic composition for skin whitening or wrinkle improvement comprising a mixture of horse oil and Rumex acetosella L. extract as an active ingredient.

The hemp oil according to the present invention may be oil refined after dissolving horse meat (refined horse oil). Preferably, the meat of the horse is cut finely and the hot plate is melted at 60-100 ° C, and then filtered using a filter paper in a dry oven. The marrow filtered through the above process may be further filtered using a filter paper in which celite and activated carbon are mixed. Through this additional filtration process, the specific odor of horses can be removed more completely and a whitish near-field oil can be produced. Further, the additional filtration process may be repeatedly performed. Since the color and the smell of the fat of the slaughtered horses vary according to the state of the horse, age and local area, it is necessary to fully understand this point when choosing the local meat. When the fat is close to the yellow color and the smell of the horse is selected, the yellow color remains even after the filtration process is completed, and the odor is so severe that it is not suitable for selection as a pharmaceutical composition or cosmetic. In this case, the horse suitable for harvesting horse meat is suitable for horse within 6 years of age. In case of using horse of 6 years or more, especially 7-10 years of age, even if horse oil is purified, It is not suitable for use as cosmetics.

The swine swimming extract according to the present invention may be obtained by isolating and obtaining an extract from swine swimming using a method of extraction and separation known in the art, It can be extracted from the baby swimming leaves, stems or outposts. Any suitable solvent that can be used to obtain the extract may be any of those accepted in the art, and water or an organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms, acetone, ether, and the like, including purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. But is not limited to.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the baby swimming extract of the present invention, and any known method can be used.

For example, the baby swim extract contained in the composition of the present invention can be used as it is, while the primary extract extracted by the above solvent extraction method is centrifuged, filtered, concentrated, freeze-dried and stored in a refrigerated room. May be prepared in a powder state by an additional process such as vacuum distillation and freeze-drying or spray-drying. In addition, the primary extract can be further fractionated using a variety of chromatographies, such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, .

Therefore, in the present invention, the swine swimming extract is a concept including all extracts, fractions, and purified products obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

The cosmetic composition of the present invention may contain 0.001 to 10% by weight of the hemp oil, based on the total weight of the composition, and may include the baby swimming extract in an amount of 0.001 to 10% by weight based on the total weight of the composition. When the above-mentioned marrow or swine swimming extract is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition, the effect of promoting wrinkles or stimulating skin regeneration may be reduced.

The formulation of the cosmetic composition of the present invention is not particularly limited, but it is preferably a formulation of an external preparation for skin, and one preferred form of the composition for external preparation for skin is a composition containing a softening agent, a nutritional lotion, a massage cream, , Gel or skin adhesive type cosmetic composition, and other preferred forms include transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays. Further, in the external preparation for each formulation, components other than the above-mentioned essential components can be mixed and selected without difficulty by those skilled in the art depending on the formulation or purpose of use of the other external preparation.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. It may be any formulation suitable for topical application, for example, as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in a water phase, a suspension microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of a warm follicular dispersion, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic compositions of the present invention can be prepared in the form of adjuvants which can be applied topically or systemically, which are conventionally used in the field of dermatology by containing dermatologically acceptable mediums or bases, Can be prepared according to the method.

In addition, the cosmetic composition of the present invention can be used as a cosmetic composition in the form of a cosmetic composition containing a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, In the field of cosmetics such as fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics And may contain commonly used adjuvants. These adjuvants are introduced in amounts commonly used in the cosmetics field.

The composition of the present invention can be formulated in the form of a liquid, an aerosol, and a sterile injectable solution. When the composition is formulated, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, Can be used.

[Formulation Example 1]

A cleanser for moisturizing and wrinkle-improving of a mixed composition of purified hemp oil and swine swimming extract

number ingredient content(%) One Sodium laureth sulfate 12.00 2 Methyl perfluorobutyl ether 3.10 3 Methyl piperyl isobutyl ether 3.10 4 Cocamito Profile Betaine 6.20 5 Phage-7 glyceryl cocoate 1.85 6 glycerin 1.85 7 Acrylate copolymer 1.50 8 Acrylate / Behenes-25 methacrylate copolymer 0.45 9 Ethylhexyl isonanoate / isononyl isononanoate 0.24 10 Potassium hydroxide 0.13 11 Polyhydroxystearic acid 0.12 12 Sodium cocamidopropylphi-dimonium chloride phosphate 0.03 13 Xanthan gum 0.10 14 Disodium cocoamphodiacetate 0.20 15 Mayu 0.02 16 Baby swimming extract 0.10 17 Preservative, fragrance a very small amount 18 Distilled water Balance

[Formulation Example 2]

Functional Essence for Moisturizing and Wrinkle Improvement of Mixed Composition of Tablet Hemp and Swine Swimming Extract

number ingredient content(%) One glycerin 6.00 2 Dipropylene glycol 6.00 3 Glycereth-26 2.50 4 Niacinamide 2.00 5 Baby swimming extract 1.00 6 Mayu 0.20 7 Trehalose 0.10 8 Betaine 0.10 9 Allantoin 0.10 10 Sodium hyaluronate 0.02 11 Adenosine, ceramide 3 a very small amount 12 Tromethamine 0.30 13 Hydrogenated lecithin a very small amount 14 Acrylate / C10-30 alkyl acrylate crosspolymer 0.15 15 Polyglyceryl-10 myristate 0.10 16 Clofenine 0.28 17 1,2-hexanediol 0.02 18 Caprylyl glycoll 0.10 19 Spices a very small amount 20 Distilled water Balance

[Formulation Example 3]

A pack for moisturizing and wrinkle improvement of a mixed composition of purified hemp milk and a baby swimming extract

number ingredient content(%) One Acrylate / C10-30 alkyl acrylate crosspolymer 17.00 2 glycerin 11.00 3 Ammonium acryloyldimethyltaurate / Vpicopolymer 10.00 4 Butylene glycol 7.00 5 Cetyl hexanoate 3.00 6 Cyclomethicone 2.00 7 Sodium hyaluronate 2.00 8 Mayu 0.20 9 Baby swimming extract 1.00 10 Cetearyl Olivate / Sorbitan Olivate 0.80 11 Dimethicone 0.50 12 Betaine 0.50 13 1,2-hexanediol 0.50 14 ethanol 0.50 15 Hydroxyacetophenone 0.30 16 Tromethamine 0.24 17 Disodium Iodide, Spice, Plant Extract a very small amount 18 Distilled water Balance

Hereinafter, the present invention will be described in more detail by way of examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Example  1 > Preparation of a mixed composition comprising purified hemp oil and swine swimming extract

The oil (refined horse oil) purified after dissolving horse meat was diluted to a concentration of 0.05%.

100 g of completely dried baby swimming was extracted with a 70% ethanol (EtOH) aqueous solution using a solvent extraction method. In order to increase the extraction efficiency, the mixture was stirred for 48 hours or more using a stirrer. The swine extract was dissolved at a concentration of 50 μg / mL. The purified swine extract was applied at a ratio of 1: 5 (V / V) ) To prepare a mixed composition.

< Example  2> Cytotoxicity Analysis of Mixture Composition Containing Purified Mayu and Swine Swimming Extracts

Murine melanoma cells (B16F10) were aliquoted in a 96-well plate at a concentration of 1 × 10 ⁵ / mL and cultured for 24 hours. Then, 100 nM of α-MSH, 40 μM of Melasolv, 0.05% (50 μg / mL), purified water and purified water, respectively. After 24 hours, the samples were removed. 100 μL of fresh medium and 10 μL of MTT solution were added to each well. CO ₂ incubator. After removing the culture medium, 100 μL of dimethylsulfoxide solution was added, and the mixture was shaken in a shaker for 10 minutes. Then, the absorbance at 570 nm was measured with an ELISA reader.

As a result, no cytotoxicity was observed in all of the extracts of α-MSH (stimulant), Melasolv (positive control), purified hemp, baby swimming and purified hemp and baby swimming (FIG.

< Example  3> Melanogenesis inhibitory effect analysis

Murine melanoma cells (B16F10), or the like cells a suitable amount of FBS (fetal bovine serum) is containing the inoculated in 6-well plate in DMEM medium 1 × 10 ⁵ pieces per well and then 5% CO _2, cells in 37 ℃ the well plate at a concentration of about 80%. After removing the culture medium, and samples were incubated under the concentration time constant 5% CO _2, 37 ℃ was replaced with the diluted medium to a suitable. After the cultured cells were removed from the culture medium, the cells were washed with PBS (phosphatized buffer saline), treated with trypsin, and the recovered cells were counted using a hematocytometer, For 10 minutes, and the supernatant was removed to obtain a pellet. The cell pellet was dried at 60 ° C., and 100 μl of 1 M sodium hydroxide solution containing 10% DMSO or an appropriate amount of cell lysis buffer was added to obtain intracellular melanin in a thermostatic chamber at 80 ° C. The melanin solution was incubated with a microplate reader at 490 nm The absorbance was measured to determine the amount of melanin.

As a result, it was shown that 15% inhibition of melanin production induced by α-MSH (100 nM) treatment and inhibition of 22% inhibition of 50% (50 μg / mL) It was confirmed that 58% of melanin formation inhibitory effect was obtained when the swimming composition was treated (Fig. 2). Therefore, it was confirmed that the melanin production inhibitory effect was much better when the purified tablet hemp and swine swimming extract mixed composition was used than the tablet hemp or swim swimming extract alone.

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< Example  5> Measurement of collagen production promotion effect

Human dermal fibroblast (pH 8-10) was added to a culture medium (DMEM medium) containing 10% fetal bovine serum and cultured at 37 ° C in a 5% CO ₂ incubator. trypsin-EDTA, and the recovered cells were divided into 24 well-plates at 5 × 10 ⁴ cells / well and stabilized for 24 hours. At this time, when samples containing the transformed growth factors (TGF-β, positive control, 10 ng / mL), purified hemp (0.05%), baby swimming (50 μg / mL) For 48 hours, and the culture medium of fibroblasts to which nothing was added was used as a control. After the culture was completed, the amount of collagen synthesis was quantified using a Procollagen type I C-peptide enzyme immunoassay (EIA) kit (MK101, Takara).

As a result, it was found that the treatment of the purified hemp (0.05%) increased the collagen production by 2.6%, the hematopoiesis (50 μg / mL) In one case, the effect of increasing collagen formation was 14.1%. Therefore, it was confirmed that the collagen production effect was remarkably improved when the mixed tablet of the purified hemp oil and the swine swimming extract was used as compared with the case of using the hemp oil or swim swimming extract alone.

< Example  6> Refined Mayu  &Lt; / RTI &gt; MMP -1 production inhibition assay

 The effect of inhibiting the production of MMP-1, a collagenase, in a mixed composition of tablets hemp and swine swimming extract was measured.

Specifically, human normal skin cells were inoculated into a 48-well microplate (Nunc, Denmark) at a concentration of 1 × 10 ⁶ cells per well, and cultured in DMEM medium (Sigma, United States of America) and 37 Incubated for 24 hours at room temperature, and then mixed with the serum-free DMEM medium supplemented with the above-mentioned addition of the purified hemp oil alone, the hemp swimming extract alone, the purified hemp milk and the baby swimming extract and the mixed composition of the purified hemp milk and the baby swimming extract In serum-free DMEM medium for 48 hours.

After culturing, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 2.

&Quot; (2) &quot;

sample Inhibition rate (%) Baby swimming extract 60 Refined Mayu 58 Purified Maeil + Swine Swimming Extract Mixture Composition 90 Retinol 30

As shown in the results of the above Table 2, it can be seen that the mixed composition of purified tablet hemp and swine swimming extract of the present invention inhibits the production of MMP-1 (inhibits MMP-1 activity) .

< Formulation Example  1> Refined Mayu  &Lt; / RTI &gt; and a baby swimming extract Cosmetics  Preparation of composition

The cosmetic composition comprising the mixed composition of the purified hemp oil and the baby swim extract was prepared in Formulation Example 1 and the cosmetic composition comprising the purified hemp oil and the baby swim extract instead of the mixed composition of the purified hemp oil and the baby swim extract (Comparative Formulation Example 3) containing glycerin and 1,3-butylene glycol as moisturizers in place of the mixed composition of tablets and tablets, and tablets (Comparative Formulation Example 4) containing adenosine as a wrinkle improving raw material instead of the mixed composition (Comparative Formulation Example 4), a cosmetic composition containing neither a mixed composition of purified water and a baby swimming extract nor a moisturizing agent (Comparative Formulation Example 5) Respectively.

division ingredient Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 A





Mixture composition of purified hemp milk and baby swimming extract 5.0 - - - - - Refined Mayu - 5.0 - - - - Baby swimming extract - - 5.0 - - - glycerin - - - 5.0 - - 1,3-butylene glycol - - - 5.0 - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 adenosine - - - - 0.04 - B



Purified water to 100 to 100 to 100 to 100 to 100 to 100 Glycereth-26 10.0 10.0 10.0 10.0 10.0 10.0 Betaine 0.5 0.5 0.5 0.5 0.5 0.5 Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 Tromethamine 1.2 1.2 1.2 1.2 1.2 1.2 Polyglyceryl-10 myristate 0.5 0.5 0.5 0.5 0.5 0.5 Clofenine 1.4 1.4 1.4 1.4 1.4 1.4 1,2-hexanediol 0.1 0.1 0.1 0.1 0.1 0.1 Caprylyl glycoll 0.5 0.5 0.5 0.5 0.5 0.5 C incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

< Example  7> Refined Mayu  Analysis of moisturizing and wrinkle-improving of mixed composition

The clinical moisturizing effect of the cosmetic composition of Formulation Example 1 was measured as follows. Sixty healthy adult men and women who felt dry skin were divided randomly into 6 groups (A, B, C, D, E and F) by 10 persons, and the cosmetic composition of Formulation Example 1 was administered to group A, The cosmetic composition of Comparative Formulation Example 2, the cosmetic composition of Comparative Formulation Example 3, the cosmetic composition of Comparative Formulation Example 2, the cosmetic composition of Comparative Formulation Example 2, the cosmetic composition of Comparative Formulation Example 2, The cosmetic composition of Example 5 was applied to the face for 2 weeks each for 8 weeks. The moisturizing effect was evaluated by using Corneometer CM 820 (Corage + Khazaka, Germany) after every 2 hours and after the test. The experimental results are shown in Table 4 below.

As a result, it was confirmed that the moisturizing effect of the cosmetic composition (Formulation Example 1) containing the purified tablet of the present invention and the composition of the present invention was excellent, as compared with Comparative Formulation Example 3 containing other moisturizing agents.

division Before use 2 weeks after use 4 weeks after use Formulation Example 1 20.5 35 42.5 Comparative Formulation Example 1 20 33.5 36 Non-formulation Example 2 20 32 34.5 Comparative Formulation Example 3 21.5 31 33 Comparative Formulation Example 4 21 31.5 32 Comparative Formulation Example 5 20 21 21.5

In addition, a clinical test was conducted on the effect of improving the skin wrinkles of the cosmetic composition containing the purified composition of the present invention and the composition of the swine swimming extract of the present invention. In clinical trials, 60 women aged 40 to 60 years were randomly divided into 6 groups (A, B, C, D, E and F) by 10 persons. The cosmetic composition of Formulation Example 1 was administered to group A, The cosmetic composition of Comparative Formulation Example 3, the cosmetic composition of Comparative Formulation Example 4, and the cosmetic composition of Comparative Formulation 5 Of the cosmetic composition were applied to the face for 2 weeks each for 8 weeks. The wrinkle improvement effect after 8 weeks was visually evaluated to confirm the wrinkle improvement degree. The effect of improving wrinkles after the completion of the experiment was evaluated by visual examination and instrument measurement (cutometer SEM 575, C + K Electronic Co., Germany) of a skilled physician before and after 3 months of use. The experimental results are shown in Table 5 below.

As a result, the cosmetic composition containing the mixed composition of the purified hemp oil and the baby swim extract showed higher wrinkle improving effect than the comparative form containing the purified hemp oil and the baby swim extract alone, and adenosine The results are similar to those of the cosmetic composition of Comparative Example 4, and thus it can be confirmed that the mixed composition of the purified hemp oil and the baby swim extract has an excellent effect of improving wrinkles.

division
Wrinkle improvement effect Effective rate (%)
Great slightly none Formulation Example 1 18 6 One 96% Comparative Formulation Example 1 10 11 4 84% Comparative Formulation Example 2 8 10 7 72% Comparative Formulation Example 4 17 7 One 96%

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (5)

A cosmetic composition for skin whitening comprising an extract of horse oil and Rumex acetosella L. as an active ingredient. A cosmetic composition for improving skin wrinkles comprising horse oil and Rumex acetosella L. extract as an active ingredient. 3. The method according to claim 1 or 2,
The baby swim extract may be obtained by using at least one extraction solvent selected from the group consisting of water, a 1-4 carbon anhydrous or hydrated lower alcohol, a polyhydric alcohol, a hydrocarbon-based solvent or a mixed solvent thereof, Wherein the cosmetic composition is an extract obtained by extracting the cosmetic composition. 3. The method according to claim 1 or 2,
Wherein the hemp oil is contained in an amount of 0.001 to 10 wt% based on the total weight of the composition, and the baby swimming extract is contained in an amount of 0.001 to 10 wt% based on the total weight of the composition. 3. The method according to claim 1 or 2,
The cosmetic composition is selected from the group consisting of lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, paste, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax and spray &Lt; / RTI &gt; or a pharmaceutically acceptable salt thereof.

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