KR101922635B1 - Cosmetic composition for improving skin inflammation comprising horse oil and sprout extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin irritation comprising an extract of horse radish and sprout as an active ingredient, wherein the mixture of at least one sprout extract selected from the group consisting of refined horse radish, rapeseed bud, perilla sprout and barley sprout of the present invention The cosmetic composition containing the composition as an active ingredient has excellent inflammation inhibiting effect and acne suppressing effect. In addition, the tablet of the present invention, the extract of the sprouts and the mixed composition of the present invention are derived from natural materials and do not cause cytotoxicity, so that they are highly utilized as a cosmetic composition.

Description

[0001] The present invention relates to a cosmetic composition for improving skin inflammation comprising horse oil and sprout extract,

The present invention relates to a cosmetic composition for improving skin inflammation comprising an extract of horse radish and sprouts as an active ingredient.

Skin aging is a complex process involving internal and external factors. In other words, there is a process of internal aging due to chronic damage due to irreversible tissue degeneracy and a process of external aging due to ultraviolet rays. In other words, ultraviolet light is the most important environmental factor that causes skin damage. When the skin is exposed to ultraviolet rays, it causes skin deformation such as cell edema. Furthermore, when chronic exposure is continued, various inflammatory factors are generated, and collagen tissue destruction in skin tissue, caratin cell proliferation and normal function of melanocytes It causes effects such as fine lines, roughness, dryness, skin sagging and pigmentation.

The inflammatory response in the skin begins as an action to defend against skin irritation caused by physical stimuli, chemicals, and bacteria, and various immune cells and inflammation-inducing cytokines are involved. IL-8 (interleukin-8) and IL-6 (interleukin-1β) are typical inflammation-inducing cytokines such as TNF-α, IL-1β and IL-8. In addition, activated macrophages overexpress inflammation-inducing cytokines as well as nitric oxide (NO) and prostaglandin E2 (PGE2), further activating inflammatory processes.

Acne vulgaris, one of the diseases that can cause inflammatory skin disorders, is an inflammatory skin disease in the sebaceous glands mainly caused by hormonal changes in puberty. The causes of acne include excessive sebum production and enlargement of sebaceous gland activated by androgen, proliferation of epidermal keratinocytes in follicle line, incomplete removal of follicular keratinocytes and clogging of fur branch line, and Propionibacterium Acnes (P. acnes) are known to be caused by bacteria. There are several forms of retinolic acid known to date that inhibit the production of sebum and prevent the spread of sebaceous glands. In addition, antibiotic therapy that suppresses the reproduction of P. acnes is also known as acne treatment. However, these retinol acids and antibiotics can cause skin irritation, irritation to the skin, and side effects such as bacterial resistance, resulting in restrictions on their use.

Under these circumstances, the present inventors have continued to develop new natural substances having an inflammation-suppressing effect and fewer human side effects, and as a result, found that Maehu and sprout extracts exhibit anti-inflammatory effects without cytotoxicity Thus completing the present invention.

Korean Patent No. 10-1561588 Korean Patent Publication No. 10-2016-0101492

Accordingly, an object of the present invention is to provide a cosmetic composition having an anti-inflammatory effect comprising a mixed composition of horse oil and sprout extract as an active ingredient.

In order to accomplish the object of the present invention as described above, the present invention provides a cosmetic composition for anti-inflammation comprising a mixed composition of horse oil and sprout extract as an active ingredient.

In one embodiment of the present invention, the hemp oil may be oil purified after dissolving horse meat.

In one embodiment of the present invention, the bud extract may be one or more extracts selected from the group consisting of rapessed sprout extract, perilla sprout extract, and barley sprout extract .

In one embodiment of the present invention, the sprout extract is prepared by using at least one extraction solvent selected from the group consisting of water, an anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent or a mixed solvent thereof .

In one embodiment of the present invention, the hemp oil may be contained in an amount of 0.001 to 10% by weight based on the total weight of the composition, and the bud extract may be contained in an amount of 0.001 to 10% by weight based on the total weight of the composition.

In one embodiment of the present invention, the composition may further comprise an acne inhibiting or improving use.

In an embodiment of the present invention, the cosmetic composition may be in the form of a lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, paste, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, , A wax, and a spray.

The cosmetic composition comprising, as an active ingredient, a mixed composition of one or more bud extracts selected from the group consisting of a horse radish sprout, a perilla sprout and a barley sprout according to the present invention is characterized by NO production and acne bacterium ( Propionibacterium Acnes inhibits the production of inflammatory cytokine IL-8 and has an antimicrobial effect against acne bacteria and has an inflammation and acne inhibiting effect. In addition, the purified tablet of the present invention, the bud extract and the mixed composition thereof are derived from natural materials and do not cause cytotoxicity, so that they can be effectively used as a cosmetic composition.

FIG. 1 is a graph showing the results of measuring the cell survival rate and the inhibitory effect of NO activity upon treatment with sprout extracts in RAW 264.7 cells; (a) LPS (1 μg / mL) and / or perilla sprout extract (12.5, 25, 50, 100 and 250 μg / mL) when treated with LPS (1 μg / mL) and / (C) treated with LPS (1 μg / mL) and / or barley sprout extracts (12.5, 25, 50, 100 and 250 μg / mL).
FIG. 2 is a graph showing the results of a single treatment or refinement of the purified herbal extract (250 μg / mL), barley bud extract (250 μg / mL) and rape bud extract (250 μg / mL) in THP- Which shows a result of measurement of cell survival rate and inflammation inhibition effect according to a mixed composition treatment in which at least one of buds, perilla sprouts and barley bud extracts are mixed; (a) Acne bacteria ( Propionibacterium acnes ), (b) MTT assay results on cell viability.

The present invention is characterized in that a cosmetic composition for improving skin inflammation and acne is provided by confirming the inflammation-inhibiting effect of marrow, rapeseed extract, perilla sprout extract, barley sprout extract, and a mixed composition thereof.

Plant germination is the most important period of the plant and various physiological activities occur. It is known that the essential amino acids, vitamins (A, C, B1, B2, B3, B5, B6) and minerals increase during this period. In addition, the secretion of gibberellin (enzymatic activity), cytokinin (promoting cleavage of cytokinin, promoting budding and infant development), and auxin (auxin, yuya kidney and actinic activity) are promoted. In other words, when seeds germinate in plant seeds, they produce various physiologically active substances as fungi, bacteria, and other enemies that defend themselves from external enemies. These substances can exhibit various functions in the human body.

In the present invention, the term "bud" means the seeds of each plant are sown on the seedling plate and sprout, or the seeds are seeded on the seedling plate after the seeds are germinated white by supplying water in the vessel, Refers to a plant of 6 to 50 mm in size harvested in 3 to 5 days in the short term, 12 to 15 days in the long term, and generally around 8 days.

The rapeseed of the present invention (Brassica napus L.) is an annual plant belonging to the cruciferous plant, which is mainly planted in the southern part of Korea and most of it is produced in Jeju Island. Studies have shown that rapeseed is effective in preventing adult diseases and has recently been shown to be effective in relieving atopic symptoms. It is also rich in vitamins A and C as well as other spring herbs. Rapessed sprout contains rapeseed oil and fatty acids, and carotene components of rapeseed are known to increase collagen synthesis in human skin.

The Perilla frutescens var. Japonica Hara of the present invention is an annual herbaceous plant belonging to the family Lamiaceae, and has a cancer and wrinkle suppressing effect. In fact, perilla sprout inhibits collagenase (MMP-1) It has been shown that it exhibits an effect of proliferating fibroblasts and has an excellent effect for improving wrinkles.

The barley (Hordeum vulgare L.) of the present invention is a crop belonging to the genus Paddy, and barley sprout is a crop of Lactobacillus sp. ), DNA damage, and ROS, and has been reported to inhibit skin cancer caused by UV.

The horse oil of the present invention is a fat ingredient extracted from the fatty tissue of horses. The Chinese medicine classical name of the medicine and the herbal ginseng root mean "hair" "And" help the blood circulation. "It is widely used as a burn remedy in Asia from ancient times to the record of being used as medicines in China in the 5th and 6th century. Has come. Major ingredients of maruyu are palmitoleic acid and ceramide. In addition, Maehu is very similar to human sebum, has high affinity with human skin, and contains about 60% or more of unsaturated fatty acids, and it is in the spotlight as a cosmetic raw material that can supply unsaturated fatty acid to skin.

The inventors of the present invention have conducted studies to develop such a horse oil extract and a bud extract as a cosmetic product. As a result, they found that the horse oil extract and sprout extract exhibited an anti-inflammatory effect and an anti-acne effect without cytotoxicity.

Particularly, in the present invention, the effect of inhibiting the NO scavenging activity and the production of IL-8, an inflammatory cytokine induced by acne bacterium ( Propionibacterium acnes ), in the case of Mayu, rapeseed extract, perilla sprout extract, barley sprout extract, And it was experimentally proved that the effect is remarkably improved when the mixed composition is used more than when the horse oil or the bud extract is used singly. The mixed composition of the refined horse oil and the sprout extract of the present invention and the cosmetic The composition proved to have antibacterial effect against acne bacteria, acne improvement and sebum secretion reduction effect, and excellent inflammation improving effect (see Examples 1 to 6).

Accordingly, the present invention is characterized by providing a cosmetic composition for anti-inflammation comprising, as an active ingredient, a mixed composition of horse oil and sprout extract.

The hemp oil according to the present invention may be oil refined after dissolving horse meat (refined horse oil). Preferably, the meat of the horse is cut finely and the hot plate is melted at 60-100 ° C, and then filtered using a filter paper in a dry oven. The marrow filtered through the above process may be further filtered using a filter paper in which celite and activated carbon are mixed. Through this additional filtration process, the specific odor of horses can be removed more completely and a whitish near-field oil can be produced. Further, the additional filtration process may be repeatedly performed. Since the color and the smell of the fat of the slaughtered horses vary according to the state of the horse, age and local area, it is necessary to fully understand this point when choosing the local meat. When the fat is close to the yellow color and the smell of the horse is selected, the yellow color remains even after the filtration process is completed, and the odor is so severe that it is not suitable for selection as a pharmaceutical composition or cosmetic. In this case, the horse suitable for harvesting horse meat is suitable for horse within 6 years of age. In case of using horses more than 6 years old, especially about 7-10 years old, even if horse oil is refined, And is not suitable for use as cosmetics.

According to one embodiment of the present invention, the bud extract may be one or more extracts selected from the group consisting of rapeseed sprout extract, perilla sprout extract, and barley sprout extract.

The method for obtaining the bud extract according to the present invention is not particularly limited, and preferably a method obtained from the bud of the plant or a method obtained by germinating the seed can be used.

The extract of the sprout according to the present invention can be obtained by isolating and obtaining an extract of sprouts using a method of extraction and separation known in the art, The prepared buds or buds can be extracted as they are or by grinding. Any suitable solvent that can be used to obtain the extract may be any of those accepted in the art, and water or an organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms, acetone, ether, and the like, including purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. But is not limited to.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the bud extract of the present invention, and any known method can be used.

For example, the sprout extract contained in the composition of the present invention may be used as it is, while the primary extract extracted by the above solvent extraction method is centrifuged, filtered, concentrated and freeze-dried and stored in a refrigerated room. And may be produced in a powder state by an additional process such as vacuum distillation and freeze-drying or spray-drying. In addition, the primary extract can be further fractionated using a variety of chromatographies, such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, .

Therefore, in the present invention, the bud extract is a concept that includes all the extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

In the examples of the present invention, extracts of rapeseed, perilla, and barley sprouts were respectively extracted with 70% ethanol (EtOH) solvent.

The cosmetic composition of the present invention may contain 0.001 to 10% by weight of the horse oil, based on the total weight of the composition, and may include the bud extract in an amount of 0.001 to 10% by weight based on the total weight of the composition. If the above extract of horse radish or sprout is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition, the inflammation inhibiting effect may be reduced.

The cosmetic composition of the present invention may further contain an application for suppressing or improving acne. At this time, the acne may be inflammatory acne, but is not limited thereto.

The formulation of the cosmetic composition of the present invention is not particularly limited, but it is preferably a formulation of an external preparation for skin, and one preferable form of the external preparation for skin is a composition containing a softening agent, a nutritional lotion, an essence, a massage cream, , Gel or skin adhesive type cosmetic composition, and other preferred forms include transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays. Further, in the external preparation for each formulation, components other than the above-mentioned essential components can be mixed and selected without difficulty by those skilled in the art depending on the formulation or purpose of use of the other external preparation.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. It may be any formulation suitable for topical application, for example, as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in a water phase, a suspension microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of a warm follicular dispersion, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic compositions of the present invention can be prepared in the form of adjuvants which can be applied topically or systemically, which are conventionally used in the field of dermatology by containing dermatologically acceptable mediums or bases, Can be prepared according to the method.

In addition, the cosmetic composition of the present invention can be used as a cosmetic composition in the form of a cosmetic composition containing a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, In the field of cosmetics such as fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics And may contain commonly used adjuvants. These adjuvants are introduced in amounts commonly used in the cosmetics field.

The composition of the present invention can be formulated in the form of a liquid, an aerosol, and a sterile injectable solution. When the composition is formulated, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, Can be used.

[Formulation Example 1] An antiinflammatory and acne improvement pack containing a mixed composition of purified tablet and sprout extract

number ingredient content(%) One Acrylate / C10-30 alkyl acrylate crosspolymer 17.00 2 glycerin 11.00 3 Ammonium acryloyldimethyltaurate / Vpicopolymer 10.00 4 Butylene glycol 7.00 5 Cetyl hexanoate 3.00 6 Cyclomethicone 2.00 7 Sodium hyaluronate 2.00 8 Barley bud extract 2.00 9 Rape bud extract 2.00 10 Perilla extract 2.00 11 Mayu 0.20 12 Cetearyl Olivate / Sorbitan Olivate 0.80 13 Dimethicone 0.50 14 Betaine 0.50 15 1,2-hexanediol 0.50 16 ethanol 0.50 17 Hydroxyacetophenone 0.30 18 Tromethamine 0.24 19 Disodium Iodide, Spice, Plant Extract a very small amount 20 Distilled water Balance

[Formulation Example 2] Anti-inflammatory and acne-cleansing cleanser containing a mixed composition of purified tablet and sprout extract

number ingredient content(%) One glycerin 20.00 2 Stearic acid 17.00 3 Potassium hydroxide 9.50 4 Laurick Acid 7.50 5 Cocamidopropyl betaine 7.00 6 Myristic acid 3.00 7 Butylene glycol 3.00 8 Glyceryl Stearate / Phage-10 2.50 9 Glyceryl stearate 2.00 10 Page-32 1.70 11 Glycol distearate 1.00 11 Barley bud extract 1.00 12 Rape bud extract 1.00 13 Perilla extract 1.00 14 Mayu 0.20 15 Cetearyl alcohol 0.50 16 Ash 0.50 17 Spices a very small amount 18 Phenoxyethanol a very small amount 19 Distilled water Balance

[Formulation Example 3] Anti-inflammation and functional essence for improving acne containing a mixed composition of purified oil and sprout extract

number ingredient content(%) One glycerin 6.00 2 Dipropylene glycol 6.00 3 Glycereth-26 2.50 4 Niacinamide 2.00 5 Barley bud extract 1.00 6 Rape bud extract 1.00 7 Perilla extract 1.00 8 Mayu 0.20 9 Trehalose 0.10 10 Betaine 0.10 11 Allantoin 0.10 12 Sodium hyaluronate 0.02 13 Adenosine, ceramide 3 a very small amount 14 Tromethamine 0.30 15 Hydrogenated lecithin a very small amount 16 Acrylate / C10-30 alkyl acrylate crosspolymer 0.15 17 Polyglyceryl-10 myristate 0.10 18 Clofenine 0.28 19 1,2-hexanediol 0.02 20 Caprylyl glycoll 0.10 21 Spices a very small amount 22 Distilled water Balance

Hereinafter, the present invention will be described in more detail by way of examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Example  1> Refined Mayu  &Lt; / RTI &gt; and bud extract

The oil (refined horse oil) purified after dissolving horse meat was diluted to 1% concentration.

100 g of completely dried rape seed, 100 g of perilla sprout and 100 g of barley sprout were respectively extracted using a 70% ethanol (EtOH) aqueous solution using a solvent extraction method. In order to increase the extraction efficiency, the mixture was stirred for 48 hours or more using a stirrer. The extracts were concentrated under reduced pressure at 50 ° C or lower and lyophilized to obtain powdery rape bud extract, perilla sprout extract and barley sprout extract. The yield of each extract was as shown in Table 1 below. Purified horse mackerel and three sprout extracts were mixed at a ratio of 1: 5 (v / v) and used as a mixed composition.

Name of sample Dry sample Extraction solvent yield Rapeseed 100g 70% EtOH 23.5% Perilla sprout 100g 70% EtOH 25.8% Barley bud 100g 70% EtOH 21.2%

< Example  2> NO scavenging activity of sprout extract

The NO scavenging activity was evaluated by measuring the nitrite concentration in the cell culture medium using a Griess reagent. The murine macrophage cell line RAW 264.7 cells were seeded on a 12-well plate at a density of 1 × 10 ⁵ cells / well and cultured in 80% confluence. Thereafter, the three sprout extracts prepared in Example 1 were treated at concentrations of 12.5, 25, 50, 100 and 250 μg / mL, respectively, and NO production was induced with LPS (1 μg / mL) for 24 hours The supernatant of the culture supernatant and Griess reagent were mixed 1: 1 and the absorbance at 540 nm was measured using an ELISA reader to evaluate the inhibitory effect of NO activity.

As a result, no cytotoxicity was observed in all of the rapeseed, perilla, and barley sprout extracts. As the treatment concentration was increased, the NO activity was generally decreased. Especially, the extracts of rapeseed and perilla sprouts showed significant NO activity at the concentration of 250 μg / (See Fig. 1). Therefore, all of the three sprout extracts were found to be effective in reducing the inflammatory response, and the NO scavenging activity of the three sprout extracts was highest in the perilla sprout extract.

< Example  3> Refined Mayu  And anti-inflammatory activity of acne sprout extract against acne

THP-1 cells, a human monocyte cell line, were purchased from the Korean Cell Line Bank and cultured in RPMI 1640 medium containing penicillin-streptomycin 100 units / mL and 10% fetal bovine serum (FBS) , 5% CO ₂ incubator and subcultured once every 3 days.

The cell viability was evaluated by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide) assay. After incubation for 24 hours with 5 × 10 ⁴ cells / mL of THP-1 cells in a 24-well plate, samples of three extracts of sprouts, which were dissolved in the hemolysate (1%) prepared in Example 1 and 250 μg / Were individually or mixed as shown in Table 2 below, and the cells were further cultured for 2 days to add 500 μg / mL of MTT to each well in an amount of 20 μl per well. After incubation at 37 ° C for 5 hours, formazan was dissolved by treatment with DMSO (dimethylsulfoxide), and the absorbance value was measured at 570 nm.

To evaluate the anti-inflammatory effect, 1 × 10 ⁵ cells / mL of THP-1 cells were cultured in a 24-well plate for 24 hours and treated with 100 μg / mL of acne bacterium ( Propionibacterium acnes ) (1%) and sprout extracts (250 μg / mL) prepared in Example 1 were treated alone or in combination as shown in Table 2 below. After incubation for 24 hours under the same conditions, the cells were removed and the amount of IL-8 in the supernatant was quantitated by ELISA.

The cell viability and the amount of IL-8 produced by the sample treatment are shown in Table 2 below.

sample IL-8 production
(pg / mL) Cell survival rate (%) One Untreated group 10.0 102.01 2 Acne bacteria (100 μg / mL) 222.5 100.00 3 Acne bacterium (100 μg / mL) + purified hemp (1%) 99.2 87.36 4 Acne bacterium (100 μg / mL) + Perilla sprout (250 μg / mL) 61.8 90.52 5 Acne bacterium (100 μg / mL) + barley bud (250 μg / mL) 64.9 91.38 6 Acne bacterium (100 μg / mL) + Rapeseed bud (250 μg / mL) 66.8 90.52 7 Acne bacterium (100 μg / mL) + purified beef (1%) + perilla sprout (250 μg / mL) 51.2 97.41 8 Acne bacterium (100 μg / mL) + purified medium (1%) + barley bud (250 μg / mL) 60.7 93.82 9 Acne bacterium (100 μg / mL) + purified beef (1%) + rapeseed bud (250 μg / mL) 49.0 94.83 10 Acne bacterium (100 μg / mL) + Purified hemp (1%) + Perilla sprout (250 μg / mL) + Barley bud (250 μg / mL) 36.1 98.28 11 Acne bacterium (100 μg / mL) + Purified hemp (1%) + Perilla sprout (250 μg / mL) + Rapeseed bud (250 μg / mL) 27.0 98.85 12 Acne bacterium (100 μg / mL) + purified beef (1%) + barley bud (250 μg / mL) + rapeseed shoot (250 μg / mL) 26.1 97.13 13 Acne sprout (250 μg / mL) + rape bud (250 μg / mL) + acne sprout (100 μg / mL) 21.0 99.45

As a result of the above experiment, it was found that perilla seed and barley sprout extract suppressed the production of IL-8 by acne bacterium (Fig. 2) when the purified horse root or sprout extract alone was treated. In addition, the use of a mixed composition in which at least one of purified oil, rape bud, perilla sprout and barley bud extract was used, as compared with the case of using the purified oil or sprout alone, further improved the inflammation inhibitory effect, It was confirmed that the combination of the buds, the barley sprouts and the rapeseed sprouts all showed the best anti-inflammatory effect.

< Example  4> Refined Mayu  And antimicrobial activity against acne bacteria

In order to confirm the antibacterial activity against the acne bacteria of the extract and the mixed composition of the present invention, a paper disc test was conducted.

Acne bacterium, which is a skin-borne bacterium that causes acne, Propionibacterium acnes ) was cultured in BHI liquid medium (Brain-Heart Infusion Broth; 3.7%) for 48 hours. The bacterial culture was plated in 0.1 mL of BHI solid medium (BHI Broth; 3.7%, agar 1.5%) and dried. Erythromycin (250 μg / mL) as a positive control and Maroochy (1%) and sprout extract (250 μg / mL) prepared in Example 1 were added to paper discs Mu] l each, then placed on the solid medium, and cultured in an anaerobic incubator at 35 [deg.] C for 3 days. The antimicrobial activity was evaluated by observing the growth inhibition zone around the paper disk and measuring the size of the inhibition zone.

The results of the antimicrobial activity test against acne bacterium were as shown in Table 3, and it was found that a mixed composition in which at least one of purified oil, rapeseed sprout, perilla sprout and barley sprout extract was mixed with each extract was used It was confirmed that the size of microbial growth inhibition was large. In particular, the antibacterial activity against acne bacterium was most excellent when a mixed composition containing a mixture of purified hemp seed, perilla sprout, barley sprout and rapeseed sprout was used.

sample Size of the growth inhibition One Erythromycin (250 μg / mL) 24mm 2 Refined beef (1%) 15mm 3 Perilla sprout (250 μg / mL) 16mm 4 Barley bud (250 μg / mL) 15mm 5 Rapeseed bud (250 μg / mL) 14mm 6 Purified beef (1%) + Perilla sprouts (250μg / mL) 17mm 7 Purified beef (1%) + barley bud (250μg / mL) 18mm 8 Purified beef (1%) + Rapeseed (250μg / mL) 17mm 9 Purified beef (1%) + Perilla sprout (250μg / mL) + Barley bud (250μg / mL) 21mm 10 (1%) + perilla sprout (250μg / mL) + rapeseed sprout (250μg / mL) 22mm 11 (1%) + barley bud (250μg / mL) + rapeseed bud (250μg / mL) 20mm 12 (250 μg / mL) + barley bud (250 μg / mL) + rape bud (250 μg / mL) 25mm

These results confirm that the purified oil of the present invention, perilla sprout, barley sprout and rape bud extract and the mixed composition thereof have an antibacterial effect against acne bacteria as well as an inflammation inhibitory effect by acne bacteria.

< Formulation Example  1> Refined Mayu  And sprout extract Cosmetics  Preparation of composition

A cosmetic composition comprising a mixed composition of at least one bud extract selected from the group consisting of rapeseed sprout extract, perilla sprout extract and barley sprout extract is shown in Table 4 Were prepared as Formulation Examples 1 to 7 as follows. In order to compare the efficacy, a cosmetic composition (Comparative Formulation Examples 1 to 4) containing a purified oil or a bud extract alone (Comparative Formulation Examples 1 to 4) and a mixed composition of a bud extract except purified oil were used instead of the mixed composition of purified oil and sprout extract (Comparative Formulation Example 6), erythromycin-containing cosmetic composition (Comparative Formulation Example 6) as a raw material for inhibiting acne, a mixed composition of purified milk and sprout extract, and an active ingredient The cosmetic composition containing no substance (Comparative Formulation Example 7) is shown in Table 5 below.

division ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 A





Refined Mayu 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Rape sprout extract 7.5 - - 3.75 3.75 - 2.5 Perilla sprout extract - 7.5 - 3.75 - 3.75 2.5 Barley sprout extract - - 7.5 - 3.75 3.75 2.5 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Erythromycin - - - - - - - B



Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 Glycereth-26 6.25 6.25 6.25 6.25 6.25 6.25 6.25 Betaine 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Allantoin 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Tromethamine 0.75 0.75 0.75 0.75 0.75 0.75 0.75 Polyglyceryl 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Clofenine 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1,2-hexanediol 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Caprylyl glycoll 0.25 0.25 0.25 0.25 0.25 0.25 0.25 C incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

division ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Comparative Formulation Example 7 A





Refined Mayu 8.0 - - - - - - Rape sprout extract - 8.0 - - 2.7 - - Perilla sprout extract - - 8.0 - 2.7 - - Barley sprout extract - - - 8.0 2.7 - - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Erythromycin - - - - - 0.2 - B



Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 Glycereth-26 6.25 6.25 6.25 6.25 6.25 6.25 6.25 Betaine 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Allantoin 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Tromethamine 0.75 0.75 0.75 0.75 0.75 0.75 0.75 Polyglyceryl 0.25 0.25 0.25 0.25 0.25 0.25 0.25 Clofenine 0.7 0.7 0.7 0.7 0.7 0.7 0.7 1,2-hexanediol 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Caprylyl glycoll 0.25 0.25 0.25 0.25 0.25 0.25 0.25 C incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

< Example  5> Refined Mayu  And sprout extracts to improve acne and skin irritation

15 persons having acne were allowed to use the cosmetic composition prepared from Formulation Examples 1 to 7 and Comparative Formulation Examples 1 to 7 for 30 days. Acne improvement scale is from 1 point to 5 points, 1 point is 'No', 3 points are 'Normal' and 5 points are 'Very agree'. The experimental results are shown in Table 6 below with an average score of 15 persons.

The acne extermination period was based on the number of days of disappearance readings, and the results were based on the results after 1 month with or without recurrence of acne. The reduction of sebaceous secretion was from 1 point to 5 points, 1 point was 'No', 3 points were 'Normal' and 5 points were 'Very agree'. The experimental results are shown in Table 6 below with an average score of 15 persons.

The presence or absence of skin irritation was evaluated by (number of stimuli) / (total number of test subjects).

Inflammatory acne improvement Acne-bloating period Recurrence of acne Decrease sebaceous glands Skin irritation Formulation Example 1 4.1 6 days radish 4.0 1/15 Formulation Example 2 4.0 6 days radish 4.1 0/15 Formulation Example 3 4.0 5 days radish 4.0 1/15 Formulation Example 4 4.3 5 days radish 4.1 0/15 Formulation Example 5 4.5 4 days radish 4.2 1/15 Formulation Example 6 4.4 4 days radish 4.3 2/15 Formulation Example 7 4.7 1 day radish 4.5 0/15 Comparative Formulation Example 1 3.1 8 days radish 3.7 0/15 Comparative Formulation Example 2 3.3 7 days radish 3.8 0/15 Comparative Formulation Example 3 3.2 7 days radish 3.7 0/15 Comparative Formulation Example 4 3.5 8 days radish 3.8 0/15 Comparative Formulation Example 5 3.7 7 days radish 3.9 1/15 Comparative Formulation Example 6 4.4 2 days radish 4.3 9/15 Comparative Formulation Example 7 1.9 14 days U 2.1 0/15

As shown in Table 6, Formulation Examples 1 to 7 showed no recurrence of acne compared to Comparative Formulation Example 7, and an average of 4 points or more for inflammatory acne improvement and an average of 6 days or less for acne- The average score of 4 or more was obtained for the reduction, and it was found that there was an excellent effect on improvement of acne. Compared to Comparative Formulation Example 7, Comparative Formulation Examples 1 to 5 also showed the effect of improving relatively acne. However, it was confirmed that the use of Formulation Examples 1 to 7 was more effective in improving acne. On the other hand, Comparative Formulation Example 6 containing a substance having an antibacterial and acne-improving effect exhibited similar effects to Formulation Examples 4 to 7, but the stimulation is stronger than that of the other experimental examples in use, Although there is concern for skin irritation, the composition according to the present invention was found to be free of irritation.

Therefore, considering the various factors such as irritation, speed of acne improvement, degree of improvement of acne, degree of decrease of sebum secretion, it was confirmed that the mixed composition of horse oil and sprout extract is suitable for acne skin patients.

< Example  6> Refined Mayu  And sprout extracts

The antiinflammatory effect of the mixed composition of purified horse meat oil and sprout extract was evaluated by inhibiting the production of prostaglandin. The effects were measured on macrophages using Formulation Examples 1 to 7 and Comparative Formulation Examples 1 to 7 prepared in Example 5 above. First, aspirin was added to the macrophage collected from mouse peritoneal cavity to a final concentration of 500 M to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells, and then the 96-well plated ml was added to each well And incubated for 2 hours in a 5% CO2 and 37 ° C incubator to attach macrophages to the surface of the container. Then, the adherent macrophages were washed three times with PBS and then used for the anti-inflammatory effect test. RPMI medium containing 1% (w / v) of LPS was added to 5x104 cells / mL of electrophoretic macrophages and cultured for 12 hours to induce the production of prostaglandins. In Formulations 1 to 7 and Comparative Formulations 1 to 7, 7 were treated with 100 μL each, and the liberated prostaglandins were quantitated by enzyme immunoassay (ELISA).

In this case, the prostaglandin production inhibitory activity of the formulation examples and the comparative formulations was determined by setting the difference of the prostaglandins produced in the LPS-treated group and the untreated group to 100%, and treating the LPS and the sample together to determine the percentage of reduced prostaglandin (Aspirin-treated group).

Prostaglandin production inhibition rate Non-treated group (Blank) 100% Control group (aspirin treatment group) 25.0% Formulation Example 1 23.9% Formulation Example 2 24.7% Formulation Example 3 24.5% Formulation Example 4 27.8% Formulation Example 5 26.7% Formulation Example 6 28.3% Formulation Example 7 39.2% Comparative Formulation Example 1 16.8% Comparative Formulation Example 2 19.2% Comparative Formulation Example 3 19.4% Comparative Formulation Example 4 18.7% Comparative Formulation Example 5 19.9% Comparative Formulation Example 6 22.2% Comparative Formulation Example 7 10.3%

As shown in Table 7, the inhibitory effect on prostaglandin production according to Formulation Examples 1 to 7 was similar to or better than the control group treated with aspirin. In the case of containing purified tablet or sprout alone (Comparative Example 1 to 4) and the case of containing a mixed composition of the bud extract except the purified tablet (Comparative Formulation Example 5), the effects of inhibiting the production of prostaglandin by Formulation Examples 1 to 7 were very high. In particular, it can be confirmed that Formulation Example 7 has the best inflammation-improving effect than the other formulation examples and comparative formulations.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

A cosmetic composition for antiinflammation comprising, as an active ingredient, a mixed composition of horse oil, rapeseed sprout extract, perilla sprout extract and barley sprout extract. The method according to claim 1,
Wherein the hemp oil is oil purified after dissolving horse meat. delete The method according to claim 1,
The extracts of rape bud, perilla sprout and barley sprout may each contain at least one extracting solvent selected from the group consisting of water, an anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, a hydrocarbon-based solvent or a mixed solvent thereof Wherein the extract is an extract obtained by using the cosmetic composition. The method according to claim 1,
The cosmetic composition according to any one of claims 1 to 3, wherein the hemp oil is contained in an amount of 0.001 to 10 wt% based on the total weight of the composition, and the rape bud extract, the perilla sprout extract and the barley sprout extract are contained in an amount of 0.001 to 10 wt% A cosmetic composition for preventing or improving acne, comprising a mixed composition of horse oil, rapeseed sprout extract, perilla sprout extract and barley sprout extract as an active ingredient. The method according to claim 6,
Characterized in that the acne is acne induced by Propionibacterium acnes .

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