KR20200064599A - A cosmetic composition for skin improvement comprising EGF and hGH as active ingredients - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement comprising an epidermal growth factor (EGF) and human growth hormone (hGH) as active ingredients. More specifically, according to the present invention, it was confirmed that a composition necessarily comprising the EGF and the hGH provides a significant effect on skin improvement including wrinkle mitigation and elasticity increasing effects. Accordingly, a composition comprising the EGF, the hGH, a plant extract, and a black yeast-fermented product can be usefully used as active ingredients of the cosmetic composition for skin improvement including skin protection, skin barrier improvement, elasticity increase, wrinkle management, and gloss effect.

Description

A cosmetic composition for skin improvement comprising EGF and hGH as active ingredients

The present invention relates to a cosmetic composition for skin improvement including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and glowing, which contains a high content of EGF (Epidermal Growth Factor) and hGH (human growth hormone).

The skin protects the human body from the external environment and is an important organ responsible for various physiological functions such as barrier function, body temperature control, and excretion to prevent the internal moisture and useful components from flowing out.

However, in general, with age, these functions gradually weaken and skin aging progresses. In particular, as the lipid composition and content of the lipid layer performing the function of the skin barrier changes, its function rapidly decreases. When the moisture content of the skin decreases, the skin dries, wrinkles form, spots, freckles, pigmentation, and Various skin lesions are caused.

The connective tissue involved in skin wrinkle formation is mainly composed of fiber components such as collagen fibers, reticular fibers, and elastic fibers.

MMP-1, which breaks down collagen and elastin in dermal tissue, is a type of matrix metalloproteinase (MMP) that is a protease present in the skin and is an important protein for cell formation, wound healing, tissue regeneration, and reorganization.

However, when exposed to ultraviolet rays, MMP-1 is produced in the body more than usual, and after binding with collagen, the collagen is cut to cause skin aging. More specifically, collagen proteins specifically bind to the'MMP-1 hemopexin domain'. Due to this, the tertiary structure of collagen is released, and after binding with zinc ions of the MMP-1 catalytic domain, it is cleaved.

Through this process, skin aging, such as wrinkles, progresses. Recently, as interest in appearance increases, studies to delay and prevent skin aging are being conducted. In addition, interest in cosmetics is increasing to maintain and improve elastic skin.

Retinol is most often used to improve skin wrinkles. Retinol is known to regulate the differentiation and regeneration of epidermal cells as a raw material announced by the Wrinkle Functional Food and Drug Administration (Griffiths CE et al., The New England Jornal of Medicine. 329(8):530-535). However, in the case of retinol, the effect is excellent, but the thermal stability is low, so it is easy to deform when heat is applied, and the toxicity is high, thereby causing skin irritation. In order to solve the instability of retinol, recently, various natural substances such as paeoniflorin extracted from peony and compound K present in ginseng as an'ECM (Extra-Cellular Matrix)' control component improve skin wrinkles It is used as a material.

As a result, the present inventors tried to develop a cosmetic for skin improvement, and as a result, a composition essentially containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) was significantly improved in skin improvement including wrinkle improvement and elasticity enhancing effect. The effect was confirmed, and the present invention was completed by revealing that EGF and hGH can be effectively used as an active ingredient in a cosmetic composition for skin improvement.

An object of the present invention is to provide a cosmetic composition for improving skin, including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and radiance, which contains a high content of EGF (Epidermal Growth Factor) and hGH (human growth hormone). It is for.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) as an active ingredient.

In addition, the present invention is Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, Lepidium meyenii Root Extract, Eriobotrya japonica Leaf Extract and Hicama Root Extract It provides at least one selected from the group consisting of Pachyrhizus erosus Root Extract, and a cosmetic composition for skin improvement further comprising a black yeast extract.

The composition comprising essentially EGF (Epidermal Growth Factor) and hGH (human growth hormone) of the present invention by confirming that it exhibits a significant effect on skin improvement including wrinkle improvement and elasticity enhancing effect, the EGF and hGH and plants The composition comprising the extract and fermentation of black yeast can be usefully used as an active ingredient in a cosmetic composition for skin improvement including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and glowing.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for improving skin containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) as active ingredients.

The EGF or hGH may be either commercially available or manufactured, and the hGH is preferably prepared as follows, but is not limited thereto:

1) After culture for 12 hours or more in LB medium using E. coli, a fermentation culture step of producing hGH through fed-batch culture after 10 hours of main culture;

2) after the fermentation culture, when the OD is 140 or higher, separating and recovering the culture medium and the cells using a continuous centrifuge to recover the cells;

3) a cell crushing step in which cells are crushed using a high pressure cell crusher to elute hGH to obtain hGH expressed in the cells;

4) obtaining hGH having a purity of 95% or more after purification using cation and anion chromatography purification to obtain pure hGH; And

5) After concentrating hGH with a purity of 95% or more to a desired concentration, exchange it with a final buffer to increase stability and store it.

On the other hand, the EGF or hGH of the present invention is preferably included in a high content, preferably, EGF is preferably contained in 0.5 to 2 ppm relative to the total weight of the composition, hGH is contained in 5 to 15 ppm relative to the total weight of the composition It is desirable to be.

In addition, it is preferable that the cosmetic composition of the present invention further comprises a plant extract and a fermented black yeast, the plant extract is Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, Maca root extract ( Lepidium meyenii Root Extract), Eriobotrya japonica Leaf Extract, and Hicama Root Extract ( Pachyrhizus erosus Root Extract) are more preferably one or more selected from the group consisting of Balsam Flower Extract, Starling Flower Extract, Maca It is more preferable to use a plant extract consisting of root extract, loquat leaf extract and hikama root extract.

The plant extract is preferably prepared by a method comprising the following steps, but is not limited to this:

1) extracting by adding an extraction solvent to the sample;

2) filtering the extract of step 1);

3) concentrating the filtrate of step 2) under reduced pressure; And

4) Drying the concentrate of step 3).

In the manufacturing method of the present invention, the plant sample of step 1) can be used without limitation being cultivated or commercially available.

In the production method of the present invention, it is preferable to use water, alcohol or a mixture thereof as the extraction solvent of step 1). It is preferable to use lower alcohols of C ₁ to C _{2 as} the alcohols, and it is preferable to use ethanol or methanol as lower alcohols. As an extraction method, it is preferable to use shaking extraction, Soxhelt extraction or reflux extraction, but is not limited thereto. The extraction solvent is preferably extracted by adding 1 to 10 times the amount of dried chrysanthemum, and more preferably by adding 2 to 3 times. The extraction temperature is preferably 20°C to 100°C, and most preferably 80°C to 100°C, but is not limited thereto. In addition, the extraction time is preferably 1 to 48 hours, more preferably 2 to 10 hours, and most preferably 2 to 5 hours, but is not limited thereto. In addition, the number of extraction is preferably 1 to 5 times, it is more preferable to repeatedly extract 3 to 4 times, and most preferably 3 times, but is not limited thereto.

In the manufacturing method of the present invention, it is preferable to use a vacuum reduced pressure concentrator or a vacuum rotary evaporator in step 3), but is not limited thereto. In addition, the drying is preferably dried under reduced pressure, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

In addition, the plant extract and black yeast fermentation product of the present invention is preferably added in an amount of 0.02 to 0.05 parts by weight based on the total weight of the composition, but is not limited thereto.

In addition, the skin improvement effect of the present invention may be any one or more effects selected from the group consisting of skin protection, skin barrier improvement, elasticity enhancement, wrinkle improvement and radiance.

In a specific embodiment of the present invention, the present inventors prepare a cream formulation comprising various plant combinations, EGF (Epidermal Growth Factor) and/or human growth hormone (hGH), and improve wrinkles for women after 30 years of age And as a result of confirming the effect of enhancing elasticity, the mixture containing essential flower extract, imperial flower extract, maca root extract, loquat leaf extract, hikama root extract, fermented black yeast, EGF and hGH, through interaction of ingredients It was confirmed that it exhibits remarkable wrinkle improvement and elasticity enhancement effect within a short time (see Table 3).

Therefore, the composition essentially comprising the EGF (Epidermal Growth Factor) and the human growth hormone (hGH) of the present invention shows a significant effect on skin improvement including wrinkle improvement and elasticity enhancing effect, and thus, with the EGF and hGH The composition comprising plant extracts and fermented black yeast can be usefully used as an active ingredient in a cosmetic composition for skin improvement, including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and radiance.

The cosmetic composition according to the present invention is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and mixtures thereof. It can be formulated in a formulation selected from the group consisting of.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing It may be formulated as cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, when the mixture of <3-8> of the present invention is used as a cosmetic composition, a basic cosmetic selected from lotion, emulsion, cream, essence, gel, pack and cleansing cream; It may be prepared in a formulation selected from the group consisting of makeup cosmetics such as foundation.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as a carrier component. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the present invention is a surfactant-containing cleansing, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the cosmetic composition of the present invention requires oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, or fragrances to be added to general skin cosmetics in addition to the ingredients described above in each formulation. Therefore, it can be used in combination as appropriate.

For example, the softening and longevity is 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and surfactants (polyethylene oleyl ether, polyoxyethylene cured castor oil, etc.) 0.05 to 2 in addition to the mixture of <3-8> of the present invention. It can be prepared to contain weight%. In addition, nutrient makeup and nourishing cream are 5-20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the mixture of <3-8> of the present invention. It can be prepared to contain 3 to 15% by weight, and the essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin and propylene glycol in addition to the mixture of <3-8> of the present invention. The massage cream is prepared by containing 30 to 70% by weight of oils such as liquid paraffin, petrolatum, and isononyl isononanoate in addition to the mixture of <3-8> of the present invention, and the pack of <3-8> of the present invention. In addition to the mixture, pigments such as kaolin, talc, zinc oxide, titanium dioxide, etc. in addition to the mixture of <3-8> of the present invention in a peel off pack or general emulsifying cosmetic containing 5 to 20% by weight of polyvinyl alcohol It can be prepared as a wash off pack containing 5 to 30% by weight.

Hereinafter, the present invention will be described in detail by examples and experimental examples.

However, the following examples and experimental examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples and experimental examples.

<Example 1> Preparation of plant extracts

After adding 16 times the amount of water to 50 g of each raw material disclosed in [Table 1], extracting hot water at 95° C. for 12 hours, filtering with filter paper (Watman grade 2, UK), and then concentrating it (EYELA N-3000, Japan). Concentrated under reduced pressure and freeze-dried at 50 ℃ to obtain hot water extraction.

number name Scientific name Extraction solvent Extraction yield (%) One Impatiens flower Impatiens balsamina Flower water 19.4 2 Brier Rosa multiflora Fruit water 18.8 3 Maca root Lepidium meyenii Root water 49.3 4 Loquat leaves Eriobotrya japonica Leaf water 20.4 5 Hikama root Pachyrhizus erosus Root water 9.6

<Example 2> Preparation of black yeast fermentation product

Black yeast mycelium ( A. pullulans var. melanogenum , KCTC No. 6081) was sold at the Microbiological Resource Center. For this cell culture, YM broth (Difco, France) and purified water were added to prepare a medium. After inoculating this medium with black yeast mycelium, it was fermented for 48 hours in a shake incubator (VS-8480, Vision, Korea). After centrifugation of the fermentation broth, the obtained filtrate was freeze-dried to obtain 0.64 g of a final powder.

<Example 3> Preparation of a mixture containing EGF (Epidermal Growth Factor) and / or hGH (human growth hormone)

<3-1> Preparation of a mixture containing balsam flower extract, starling flower extract, maca root extract and EGF

A mixture comprising the impatiens flower extract prepared in <Example 1>, the starling flower extract, the maca root extract and EGF was prepared.

Specifically, 200 g of each of the impatiens flower extract prepared in <Example 1>, the pink flower extract, and the maca root extract were mixed, and then 100 g of EGF (Liquid) was mixed to prepare a mixture.

<3-2> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, EGF and hGH

After mixing 200 g of each of the impatiens flower extract prepared in <Example 1>, the starling flower extract, and the maca root extract, 100 g of EGF and 1 kg of hGH (Liquid) were mixed to prepare a mixture.

<3-3> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract and EGF

Balsam flower extract prepared in <Example 1>, Brier extract, maca root extract and loquat leaf extract were mixed 200 g each, and then 100 g EGF was mixed to prepare a mixture.

<3-4> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract, EGF and hGH

Balsam flower extract prepared in <Example 1>, The mixture was prepared by mixing 200 g of Brier extract, maca root extract, and Loquat leaf extract, and then mixing 100 g of EGF and 1 kg of hGH.

<3-5> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract, hikama root extract and EGF

After mixing 200 g of each of the impatiens flower extract prepared in <Example 1>, the starling flower extract, the maca root extract, the loquat leaf extract, and the hikama root extract, respectively, 100 g of EGF was mixed to prepare a mixture.

<3-6> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract, hikama root extract, EGF and hGH

After mixing 200 g of each of the impatiens flower extract prepared from <Example 1>, the starling flower extract, the maca root extract, the loquat leaf extract, and the hikama root extract, 100 g of EGF and 1 kg of hGH are mixed and mixed. Was prepared.

<3-7> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract, hikama root extract, fermented black yeast and EGF

Black yeast fermentation product prepared in <Example 2> after mixing 200 g of each of the impatiens flower extract prepared in <Example 1>, starling flower extract, maca root extract, loquat leaf extract, and hikama root extract, respectively A mixture was prepared by mixing 200 g, 100 g of EGF.

<3-8> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat leaf extract, hikama root extract, fermented black yeast, EGF and hGH

Black yeast fermentation product prepared in <Example 2> after mixing 200 g of each of the impatiens flower extract prepared in <Example 1>, starling flower extract, maca root extract, loquat leaf extract, and hikama root extract, respectively A mixture was prepared by mixing 200 g, 100 g EGF and 1 kg hGH.

<Example 4> Preparation of cream formulation

A cream formulation was prepared using the above Example <3-1>, and the cream formulation was prepared with the components and contents of [Table 2] below. In addition, a comparative group cream formulation was prepared with the components and contents of [Table 2] using a mixture of 100 g of EGF and 1 kg of hGH as a comparison group.

In addition, in the same manner as above, a cream formulation was prepared using the mixture of Examples <3-2> to <3-8>.

ingredient Content (unit: weight%) Mixture of any one of <3-1> to <3-8> above 3.0 Lipophilic glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hydrogenated vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Preservative, fragrance, coloring a very small amount Distilled water Balance

<Experiment 1> Confirmation of wrinkle improvement and elasticity enhancement effect

The effect of improving wrinkles and improving elasticity of the cream formulation comprising the mixture of Examples <3-1> to <3-8> prepared in <Example 4> was confirmed.

Specifically, 90 women after 30 years of age were classified into 9 groups, and after washing the face, water was removed, and then the mixture of Examples <3-1> to <3-8> prepared in <Example 4> The cream formulation containing and the control cream formulation were evenly distributed on the face twice a day for 15 days.

Then, in order to confirm the effect of improving wrinkles and improving elasticity, a 7-point scale was used for evaluation, and the effect of improving wrinkles and improving elasticity was set to be higher as the score increased.

As a result, as shown in Table 3, the impregnated flower extract, starling flower extract, maca root extract, loquat leaf extract, hikama root extract, black yeast fermentation, mixtures essentially containing EGF and hGH interact the components. Through this, it was confirmed that it exhibits remarkable wrinkle improvement and elasticity improvement effect in a short time.

Specifically, when EGF and hGH were used together, the overall effect was superior to that of the group using only EGF. Especially, when all 5 plant extracts and black yeast fermentation were included, remarkable skin improvement due to synergistic effect It was confirmed that it showed an effect.

Accordingly, it was confirmed that when EGF and hGH were mixed with the plant extract and used for cosmetic use, it exhibited an excellent skin improvement effect (Table 3).

sample wrinkle improvement Elasticity enhancement effect Cream formulation using mixture of <3-1> 2.98±0.78 3.01±0.25 Cream formulation using mixture of <3-2> 3.04±0.13 3.41±0.13 Cream formulation using a mixture of <3-3> 3.75±0.13 3.45±0.23 Cream formulation using mixture of <3-4> 5.37±0.23 5.33±0.25 Cream formulation using a mixture of <3-5> 4.53±0.78 4.24±0.12 Cream formulation using mixture of <3-6> 4.98±0.48 4.35±0.24 Cream formulation using mixture of <3-7> 3.01±0.17 3.28±0.17 Cream formulation using <3-8> mixture 6.12±0.23 6.32±0.32 Cream formulation containing only EGF and hGH 4.04±0.62 4.14±0.72

<Production Example 1> Preparation of Ampoul formulation containing EGF and hGH

An Ampoul formulation containing 1 ppm of EGF and 10 ppm of hGH was prepared using the components and contents of [Table 4].

ingredient Content weight (%) Water 36.4370 PEG-8 Caprylic/Capric Glycerides 26.0000 Polyglyceryl 6-Isostearate 13.0000 Dicaprylyl Carbonate 5.0000 Cyclopentasiloxane 2.2750 Cyclohexasiloxane 1.2250 Squalane 1.0000 Parfum 0.1000 Dipropylene Glycol 12.0000 Chlorphenesin 0.2000 Adenosine 0.0400 Water 1.5160 Butylene Glycol 0.4000 1,2-Hexanediol 0.0600 Impatiens Balsamina Flower Extract 0.0040 Rosa Multiflora Fruit Extract 0.0040 Lepidium Meyenii Root Extract 0.0040 Eriobotrya Japonica Leaf Extract 0.0040 Pachyrhizus Erosus Root Extract 0.0040 Aureobasidium Pullulans Ferment 0.0040 1,2-Hexanediol 0.5000 sh-Oligopeptide-1 0.0001 Water 0.0999 Water 0.1101 sh-Polypeptide-7 0.0010 Glycine 0.0004 Mannitol 0.0111 Sodium Phosphate 0.0005

Claims (10)

EGF (Epidermal Growth Factor) and hGH (human growth hormone) cosmetic composition for skin improvement containing as an active ingredient.
[6] The cosmetic composition for improving skin according to claim 1, wherein the EGF is contained in an amount of 0.5 to 2 ppm based on the total weight of the composition.
According to claim 1, The hGH is a cosmetic composition for improving skin, characterized in that contained in 5 to 15 ppm relative to the total weight of the composition.
According to claim 1, wherein the composition is a cosmetic composition for improving skin, characterized in that it further comprises a plant extract and fermented black yeast.
The method of claim 4, wherein the plant extract is Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, Lepidium meyenii Root Extract, Eriobotrya japonica Leaf Extract and Cosmetic composition for skin improvement, characterized in that at least one selected from the group consisting of hikama root extract ( Pachyrhizus erosus Root Extract).
The cosmetic composition for skin improvement according to claim 5, wherein the extract is extracted with water, a lower alcohol of C ₁ to C ₂ or a mixed solvent thereof.
[6] The cosmetic composition for improving skin according to claim 4, wherein the plant extract and fermented yeast yeast are added in an amount of 0.02 to 0.05 parts by weight based on the total weight of the composition.
The cosmetic composition for skin improvement according to claim 1, wherein the skin improvement is at least one selected from the group consisting of skin protection, skin barrier improvement, elasticity enhancement, wrinkle improvement, and gloss application.
The method of claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray and these Cosmetic composition for skin improvement, characterized in that it has a formulation selected from the group consisting of a mixture of.
EGF (Epidermal Growth Factor), hGH (human growth hormone), plant extract and cosmetic composition for wrinkle improvement containing black yeast fermentation as an active ingredient.