KR20230152972A - Functional cosmetic composition containing EGF as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) as active ingredients. Specifically, the composition essentially containing EGF and hGH of the present invention improves wrinkles and improves elasticity. It was confirmed that it has a significant effect on skin improvement, including an enhancement effect, and the composition containing EGF and hGH, plant extracts, and black yeast fermentation protects the skin, improves the skin barrier, improves elasticity, manages wrinkles, and imparts radiance. It can be usefully used as an active ingredient in a cosmetic composition for improving skin containing it.

Description

Functional cosmetic composition containing EGF as an active ingredient}

The present invention relates to a cosmetic composition for skin improvement containing a high content of EGF (Epidermal Growth Factor) for skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and radiance.

The skin is an important organ that protects the human body from the external environment and is responsible for various physiological functions such as barrier function that prevents internal moisture and useful ingredients from leaking out, body temperature regulation, and excretion.

However, in general, as we age, these functions gradually weaken and skin aging progresses. In particular, as the lipid composition and content of the lipid layer that performs the function of the skin barrier changes, its function rapidly deteriorates. When the skin moisture content decreases, the skin becomes dry, wrinkles form, spots, freckles, pigmentation, and A variety of skin lesions are caused.

Connective tissue, which is involved in the formation of skin wrinkles, is mainly composed of fibrous components such as collagen fibers, reticular fibers, and elastin fibers.

MMP-1, which decomposes collagen and elastin in dermal tissue, is a type of MMP (matrix metalloproteinase), a proteolytic enzyme present in the skin, and is an important protein in intercellular morphogenesis, wound healing, tissue regeneration, and reorganization.

However, when exposed to ultraviolet rays, MMP-1 is produced in greater quantities than usual in the body, binds to collagen, and then cuts collagen, causing skin aging. More specifically, collagen proteins specifically bind to the 'MMP-1 hemopexin domain'. As a result, the tertiary structure of collagen is solved, and after binding to the zinc ion of the catalytic domain of MMP-1, it is cleaved.

Through this process, skin aging, including the development of wrinkles, progresses. Recently, as interest in appearance has increased, research is being conducted to delay and prevent skin aging. In addition, interest in cosmetics to maintain and improve elastic skin is increasing.

The most commonly used drug to improve skin wrinkles is retinol. Retinol, a functional ingredient for wrinkles notified by the Ministry of Food and Drug Safety, is known to regulate differentiation and regeneration of epidermal cells (Griffiths CE et al., The New England Jornal of Medicine. 329(8):530-535). However, in the case of retinol, although it is effective, it has low thermal stability, so it is easily deformed when heat is applied, and has high toxicity, causing skin irritation. To solve the instability of retinol, various natural substances such as paeoniflorin extracted from peony and compound K present in ginseng have recently been used as 'ECM (Extra-Cellular Matrix)' regulating ingredients to improve skin wrinkles. It is used as a material.

Accordingly, the present inventors made efforts to develop cosmetics for skin improvement, and as a result, a composition essentially containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) was found to have significant skin improvement effects, including wrinkle improvement and elasticity enhancement effects. It was confirmed that the results were effective, and the present invention was completed by revealing that EGF and hGH can be effectively used as active ingredients in cosmetic compositions for skin improvement.

The purpose of the present invention is to provide a cosmetic composition for skin improvement containing a high content of EGF (Epidermal Growth Factor) and hGH (human growth hormone), including skin protection, skin barrier improvement, elasticity promotion, wrinkle management, and radiance imparting. It is for.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) as active ingredients.

In addition, the present invention includes Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, Lepidium meyenii Root Extract, Eriobotrya japonica Leaf Extract, and Jicama Root Extract ( Provided is a cosmetic composition for improving skin, further comprising at least one selected from the group consisting of Pachyrhizus erosus Root Extract, and black yeast extract.

By confirming that the composition essentially containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) of the present invention has a significant effect on skin improvement, including wrinkle improvement and elasticity enhancement effects, it was confirmed that the EGF and hGH and plant Compositions containing extracts and fermented black yeast can be usefully used as active ingredients in cosmetic compositions for skin improvement, including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and radiance imparting.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for improving skin containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) as active ingredients.

The EGF or hGH can be either commercially available or manufactured, and the hGH is preferably prepared as follows, but is not limited thereto:

1) Fermentation culture step of producing hGH through fed-batch culture using E. coli after seed culture in LB medium for more than 12 hours, followed by main culture for 10 hours;

2) After fermentation culture, when the OD reaches 140 or higher, separate and recover the culture medium and the cells using a continuous centrifuge to recover the cells;

3) a cell crushing step in which the cells are disrupted using a high-pressure cell disruptor to elute hGH in order to obtain hGH expressed in the cells;

4) purifying hGH using cation and anion chromatography purification to obtain pure hGH, and then obtaining hGH with a purity of 95% or more; and

5) Step of concentrating hGH with a purity of 95% or higher to the desired concentration and then exchanging it with a final buffer and storing it to increase stability.

Meanwhile, the EGF or hGH of the present invention is preferably contained in a high content, preferably EGF is contained at 0.5 to 2 ppm based on the total weight of the composition, and hGH is contained at 5 to 15 ppm based on the total weight of the composition. It is desirable to be

In addition, the cosmetic composition of the present invention preferably further includes a plant extract and a fermented black yeast, and the plant extract includes Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, and Maca Root Extract. ( Lepidium meyenii Root Extract), loquat leaf extract ( Eriobotrya japonica Leaf Extract), and jicama root extract ( Pachyrhizus erosus Root Extract). More preferably, it is at least one selected from the group consisting of impatiens flower extract, wild rose extract, and maca. It is more preferable to use plant extracts consisting of root extract, loquat leaf extract and jicama root extract.

The plant extract is preferably prepared by a method comprising the following steps, but is not limited thereto:

1) Extracting by adding an extraction solvent to the sample;

2) filtering the extract of step 1);

3) Concentrating the filtrate of step 2) under reduced pressure; and

4) Drying the concentrate of step 3).

In the production method of the present invention, the plant sample in step 1) can be used without limitation, either cultivated or commercially available.

In the production method of the present invention, it is preferable to use water, alcohol, or a mixture thereof as the extraction solvent in step 1). The alcohol is preferably a C ₁ to C ₂ lower alcohol, and the lower alcohol is preferably ethanol or methanol. The extraction method is preferably shaken extraction, Soxhelt extraction, or reflux extraction, but is not limited thereto. It is preferable to extract by adding 1 to 10 times the amount of the extraction solvent as the dried chrysanthemum, and more preferably to extract by adding 2 to 3 times the amount of the dried chrysanthemum. The extraction temperature is preferably 20°C to 100°C, and most preferably 80°C to 100°C, but is not limited thereto. In addition, the extraction time is preferably 1 to 48 hours, more preferably 2 to 10 hours, and most preferably 2 to 5 hours, but is not limited thereto. In addition, the number of extractions is preferably 1 to 5 times, more preferably 3 to 4 times, and most preferably 3 times, but is not limited thereto.

In the production method of the present invention, the vacuum concentration in step 3) is preferably performed using a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, drying is preferably performed by reduced pressure drying, vacuum drying, boiling drying, spray drying, or freeze drying, but is not limited thereto.

In addition, the plant extract and fermented black yeast of the present invention are preferably added in an amount of 0.02 to 0.05 parts by weight based on the total weight of the composition, but are not limited thereto.

In addition, the skin improvement effect of the present invention may be any one or more effects selected from the group consisting of skin protection, skin barrier improvement, elasticity improvement, wrinkle improvement, and radiance.

In a specific embodiment of the present invention, the present inventors prepared a cream formulation containing various plant combinations, Epidermal Growth Factor (EGF), and/or human growth hormone (hGH), and then used it to improve wrinkles in women over the age of 30. As a result of confirming the effect of improving elasticity, the mixture essentially containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract, fermented black yeast, EGF, and hGH was obtained through the interaction of the ingredients. It was confirmed that it showed significant wrinkle improvement and elasticity improvement effects within a short period of time (see Table 3).

Therefore, the composition essentially containing EGF (Epidermal Growth Factor) and hGH (human growth hormone) of the present invention exhibits significant effects on skin improvement, including wrinkle improvement and elasticity enhancement effects, and therefore, the EGF and hGH Compositions containing plant extracts and fermented black yeast can be useful as active ingredients in cosmetic compositions for skin improvement, including skin protection, skin barrier improvement, elasticity enhancement, wrinkle management, and radiance imparting.

The cosmetic composition according to the present invention can be used as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing agent, oil, powder foundation, emulsion foundation, wax foundation, spray, and mixtures thereof. It may be formulated in a dosage form selected from the group consisting of:

The cosmetic composition according to the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing It can be formulated as cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited to this. More specifically, when the mixture of <3-8> of the present invention is used as a cosmetic composition, it includes basic cosmetics selected from lotions, emulsions, creams, essences, gels, packs, and cleansing creams; It can be manufactured in a formulation selected from the group consisting of makeup cosmetics such as foundation.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide ether. Sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, the cosmetic composition of the present invention contains oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, or fragrance mixed in general skin cosmetics in addition to the ingredients described above in each formulation. It can be used by mixing appropriately.

For example, in addition to the mixture of <3-8> of the present invention, the softening lotion contains 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.) It can be manufactured to contain % by weight. In addition, nutritional lotion and nourishing cream contain 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the mixture of <3-8> of the present invention. It can be prepared to contain 3 to 15% by weight, and the essence can be prepared to contain 5 to 30% by weight of polyhydric alcohols such as glycerin and propylene glycol in addition to the mixture of <3-8> of the present invention. The massage cream is manufactured by containing 30 to 70% by weight of oils such as liquid paraffin, petroleum jelly, and isononyl isononanoate in addition to the mixture of <3-8> of the present invention, and the pack is made of the mixture of <3-8> of the present invention. In addition to the mixture, pigments such as kaolin, talc, zinc oxide, titanium dioxide, etc. in addition to the mixture of <3-8> of the present invention in a peel-off pack or general emulsion type cosmetic containing 5 to 20% by weight of polyvinyl alcohol. It can be manufactured as a wash off pack containing 5 to 30% by weight.

Hereinafter, the present invention will be described in detail through examples and experimental examples.

However, the following examples and experimental examples only illustrate the present invention, and the content of the present invention is not limited to the following examples and experimental examples.

<Example 1> Preparation of plant extract

16 times the amount of water was added to 50 g of each of the raw materials shown in [Table 1] below, subjected to hot water extraction at 95°C for 12 hours, filtered through filter paper (Watman grade 2, UK), and then concentrated using a concentrator (EYELA N-3000, Japan). Hot water extraction was obtained by concentration under reduced pressure and freeze-drying at 50°C.

number name scientific name Extraction solvent Extraction yield (%) One impatiens flower Impatiens balsamina Flower water 19.4 2 briar Rosa multiflora Fruit water 18.8 3 maca root Lepidium meyenii Root water 49.3 4 loquat tree leaves Eriobotrya japonica Leaf water 20.4 5 jicama root Pachyrhizus erosus Root water 9.6

<Example 2> Preparation of black yeast fermentation product

Black yeast mycelium ( A. pullulans var. melanogenum , KCTC No. 6081) was purchased from the Microbial Resource Center. For culturing these bacteria, a medium was prepared by adding YM broth (Difco, France) and purified water. After inoculating black yeast mycelium into this medium, it was fermented for 48 hours in a shaking incubator (VS-8480, Vision, Korea). After centrifuging this fermentation broth, the obtained filtrate was freeze-dried to obtain 0.64 g of final powder.

<Example 3> Preparation of a mixture containing Epidermal Growth Factor (EGF) and/or human growth hormone (hGH)

<3-1> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract and EGF

A mixture containing the impatiens flower extract, starling flower extract, maca root extract, and EGF prepared in <Example 1> was prepared.

Specifically, 200 g each of the impatiens flower extract, starling flower extract, and maca root extract prepared in <Example 1> were mixed, and then 100 g of EGF (Liquid) was mixed to prepare a mixture.

<3-2> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, EGF and hGH

A mixture was prepared by mixing 200 g each of the impatiens flower extract, wild rose extract, and maca root extract prepared in <Example 1>, followed by mixing 100 g of EGF and 1 kg of hGH (Liquid).

<3-3> Preparation of a mixture containing impatiens flower extract, starling flower extract, maca root extract, loquat tree leaf extract and EGF

Balsam flower extract prepared in <Example 1> above, A mixture was prepared by mixing 200 g of each of the wild rose extract, maca root extract, and loquat leaf extract and then mixing 100 g of EGF.

<3-4> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, EGF and hGH

Balsam flower extract prepared in <Example 1> above, A mixture was prepared by mixing 200 g each of wild rose extract, maca root extract, and loquat leaf extract, followed by mixing 100 g of EGF and 1 kg of hGH.

<3-5> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract and EGF

A mixture was prepared by mixing 200 g of each of the impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, and jicama root extract prepared in <Example 1>, and then mixing 100 g of EGF.

<3-6> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract, EGF and hGH

After mixing 200 g each of the impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, and jicama root extract prepared in <Example 1>, 100 g of EGF and 1 kg of hGH were mixed to form a mixture. was manufactured.

<3-7> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract, black yeast fermentation product, and EGF

After mixing 200 g each of the impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, and jicama root extract prepared in <Example 1>, the black yeast fermentation product prepared in <Example 2> was mixed. A mixture was prepared by mixing 200 g and 100 g of EGF.

<3-8> Preparation of a mixture containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract, black yeast fermentation product, EGF and hGH

After mixing 200 g each of the impatiens flower extract, wild rose extract, maca root extract, loquat tree leaf extract, and jicama root extract prepared in <Example 1>, the black yeast fermentation product prepared in <Example 2> was mixed. A mixture was prepared by mixing 200 g, 100 g of EGF, and 1 kg of hGH.

<Example 4> Preparation of cream formulation

A cream formulation was prepared using Example <3-1>, and the cream formulation was prepared using the ingredients and contents shown in [Table 2] below. In addition, as a comparison group, a cream formulation of the comparison group was prepared using a mixture of 100 g of EGF and 1 kg of hGH with the ingredients and contents shown in [Table 2].

In addition, a cream formulation was prepared using the mixture of Examples <3-2> to <3-8> using the same method as above.

ingredient Content (unit: weight%) A mixture of any one of Examples <3-1> to <3-8> 3.0 Lipophilic glycerin monostearate 2.0 Cetearyl alcohol 2.2 stearic acid 1.5 beeswax 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 hydrogenated vegetable oil 1.0 squalane 3.0 mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium Magnesium Silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium Hyaluronate 4.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount

<Experimental Example 1> Confirmation of wrinkle improvement and elasticity enhancement effect

The wrinkle improvement and elasticity enhancement effects of the cream formulation containing the mixture of Examples <3-1> to <3-8> prepared in <Example 4> were confirmed.

Specifically, 90 women over the age of 30 were classified into 9 groups, washed and dried, and then mixed with the mixture of Examples <3-1> to <3-8> prepared in <Example 4>. The cream formulation containing and the control cream formulation were evenly distributed and applied to the face twice a day for 15 days.

Then, to confirm the wrinkle improvement and elasticity enhancement effect, a 7-point scale was used for evaluation, and the wrinkle improvement and elasticity enhancement effect was set to be more preferred as the score increased.

As a result, as shown in Table 3, the mixture essentially containing impatiens flower extract, wild rose extract, maca root extract, loquat leaf extract, jicama root extract, black yeast fermentation product, EGF, and hGH showed no interaction of ingredients. It was confirmed that it showed significant wrinkle improvement and elasticity enhancement effects in a short period of time.

Specifically, when EGF and hGH are used together, an overall superior effect was shown compared to the group using only EGF. In particular, when all five plant extracts and black yeast fermentation were included, the synergistic effect resulted in significant skin improvement. It was confirmed that it was effective.

Therefore, it was confirmed that when EGF and hGH were mixed with the above plant extract and used for cosmetic purposes, it showed excellent skin improvement effect (Table 3).

sample wrinkle improvement Elasticity improvement effect Cream formulation using the mixture of <3-1> 2.98±0.78 3.01±0.25 Cream formulation using the mixture of <3-2> 3.04±0.13 3.41±0.13 Cream formulation using a mixture of <3-3> 3.75±0.13 3.45±0.23 Cream formulation using a mixture of <3-4> 5.37±0.23 5.33±0.25 Cream formulation using a mixture of <3-5> 4.53±0.78 4.24±0.12 Cream formulation using a mixture of <3-6> 4.98±0.48 4.35±0.24 Cream formulation using a mixture of <3-7> 3.01±0.17 3.28±0.17 Cream formulation using a mixture of <3-8> 6.12±0.23 6.32±0.32 Cream formulation containing only EGF and hGH 4.04±0.62 4.14±0.72

<Preparation Example 1> Preparation of Ampoul formulation containing EGF and hGH

An Ampoul formulation containing 1 ppm of EGF and 10 ppm of hGH was prepared using the ingredients and contents shown in Table 4 below.

ingredient Content weight (%) Water 36.4370 PEG-8 Caprylic/Capric Glycerides 26.0000 Polyglyceryl 6-Isostearate 13.0000 Dicaprylyl Carbonate 5.0000 Cyclopentasiloxane 2.2750 Cyclohexasiloxane 1.2250 Squalane 1.0000 Parfum 0.1000 Dipropylene Glycol 12.0000 Chlorphenesin 0.2000 Adenosine 0.0400 Water 1.5160 Butylene Glycol 0.4000 1,2-Hexanediol 0.0600 Impatiens Balsamina Flower Extract 0.0040 Rosa Multiflora Fruit Extract 0.0040 Lepidium Meyenii Root Extract 0.0040 Eriobotrya Japonica Leaf Extract 0.0040 Pachyrhizus Erosus Root Extract 0.0040 Aureobasidium Pullulans Ferment 0.0040 1,2-Hexanediol 0.5000 sh-Oligopeptide-1 0.0001 Water 0.0999 Water 0.1101 sh-Polypeptide-7 0.0010 Glycine 0.0004 Mannitol 0.0111 Sodium Phosphate 0.0005

Claims (5)

A cosmetic composition for skin improvement containing EGF (Epidermal Growth Factor) as an active ingredient.
The cosmetic composition for skin improvement according to claim 1, wherein the EGF is contained in an amount of 0.5 to 2 ppm based on the total weight of the composition.
The cosmetic composition for skin improvement according to claim 1, wherein the composition further comprises plant extract and fermented black yeast. The method of claim 3, wherein the plant extract is Impatiens balsamina Flower Extract, Rosa multiflora Fruit Extract, Lepidium meyenii Root Extract, Eriobotrya japonica Leaf Extract, and A cosmetic composition for improving skin, characterized in that it contains at least one selected from the group consisting of jicama root extract ( Pachyrhizus erosus Root Extract).
The cosmetic composition for skin improvement according to claim 4, wherein the extract is extracted with water, C ₁ to C ₂ lower alcohol, or a mixed solvent thereof.