KR100570096B1 - Cosmetic Compostion for Preventing Skin Aging Comprising Nanoliposome Incorporating a Combined Extract of Oriental Medicines Containing Cervus elaphus - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging including nanoliposomes stabilized by herbal extracts, and more particularly, to a nanoliposome stabilized mixed extract of licorice (Glycyrrhiza glabra), deer antler, wolfberry and Angelica as an active ingredient. It relates to a skin aging prevention cosmetic composition comprising. The cosmetic composition of the present invention is very effective in preventing skin aging through skin wrinkle improvement and skin elasticity improvement, moisturizing ability and skin immunity enhancement by promoting the proliferation of fibroblasts and keratinocytes, collagen synthesis, as well as irritation to the skin. Since it is small, it is a cosmetic composition which does not have a side effect on skin. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Skin aging, herbal extract, licorice, antler, donkey, wolfberry, wrinkle, moisture, cosmetic, nano liposome

Description

Cosmetic composition for preventing skin aging comprising nanoliposomes containing an herbal herbal extract including antler {Cosmetic Compostion for Preventing Skin Aging Comprising Nanoliposome Incorporating a Combined Extract of Oriental Medicines Containing Cervus elaphus}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition for preventing skin aging including nano liposomes stabilized herbal extract.

The skin is in direct contact with the external environment and serves as an important barrier to protecting the body from physical, chemical and biological stimuli. However, the skin loses its original function due to oxidation of biological components due to overexposure of UV rays, deterioration of physiological function of the skin according to age, and nutritional deficiency. It shows various skin aging phenomena such as dullness. Therefore, in the development of cosmetics research to prevent or delay the aging of the skin occupies a very important position.

Recently, various skin physiological activities of natural plant components have been searched to develop materials having excellent skin beauty effects, and the use of functional cosmetics such as anti-aging is increasing rapidly. However, most herbal ingredients are not only unstable by themselves, but also have a big problem in the stability of cosmetics, such as the settling of the contents, color change, etc. when applying cosmetics.

In order to solve these problems, pharmaceutical, cosmetics and food fields are using liposomes that can stably deliver bioactive components without destroying them, and can contain both fat-soluble and water-soluble bioactive components. Recently, with the development of nano technology, research and development on nano liposomes that can maximize the skin penetration effect is actively progressing.

The present inventors have diligently researched to develop a cosmetic composition for preventing skin aging, that is, skin protection using herbal medicines in private and oriental medicine, and found that licorice, antler, goji berry, donkey, and extract have excellent physiological activity effects. In addition, these active ingredients remain stable in the cosmetic composition, so as to eliminate all the aging factors such as wrinkles, loss of elasticity and loss of moisturizing ability when applying the skin, to maximize the physiological activity effect, the extract is nanoliposomes Formulation stabilized in to develop a cosmetic composition for preventing skin aging.                         

Accordingly, an object of the present invention to provide a cosmetic composition for preventing skin aging comprising nanoliposomes stabilized herbal extract as an active ingredient.

According to the aspect of the invention there is provided the skin containing nanoliposome a stabilizing mixture extract of licorice (Glycyrrhiza glabra), antler (Cervus elaphus), Lycium chinense (Lycium chinensis) and Angelica (Angelica acutiloba kitag) as an active component aging It provides a cosmetic composition for prevention.

The present inventors conducted a study on the herbal medicines native to Korea in order to improve the problems of the conventional anti-aging (or skin protection) cosmetic composition and to further add anti-aging efficacy, as a result licorice, deer antler, goji berry, donkey extract It promotes the synthesis of collagen, which is an ingredient that gives elasticity to the skin, promotes the proliferation of fibroblasts and keratinocytes, which are mainly composed of collagen, and also shows the anti-inflammatory effect of the skin, and has little skin irritation and side effects. Furthermore, when encapsulation of the above-mentioned herbal extracts in nanoliposomes was found, the moisturizing power of the skin was greatly increased, the skin elasticity was improved, the skin penetration effect was greatly increased, and the stability of the formulation was also greatly improved. .

The present invention provides a cosmetic composition for preventing skin aging stabilized by inclusion of licorice, antler, wolfberry and Angelica extract as active ingredients in nanoliposomes.

The herbal extract of the present invention can be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol). , Butanol, etc.), (c) a mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol as an extraction solvent. can do.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the herbal extract is 0.1-20% by weight based on the total weight of nanoliposomes, more preferably 4-12% by weight.

As used herein, the term nanoliposomes refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 30-70 nm. When the average particle diameter of the nanoliposomes exceeds 70 nm, the improvement of skin penetration and the stability of formulation is very weak among the technical effects to be achieved in the present invention.

The nanoliposomes of the present invention are prepared by a mixture comprising polyols, surfactants, phospholipids, fatty acids and water, and are characterized by not using monohydric alcohols (eg ethanol).

The polyol used in the nanoliposome of the present invention is not particularly limited, and preferably, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, ziitol and Sorbitol, most preferably propylene glycol. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less phosphadidylethanolamine. In the preparation of the nanoliposomes of the present invention, the amount of lecithin is 0.5 to 20% by weight, preferably 2 to 8% by weight based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It includes. The amount used is 0.05-3.0 wt% with respect to the total weight of nanoliposomes, preferably 0.1-1.0 wt%.

The water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is 5.0-40% by weight based on the total weight of the nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposome system thus prepared has the advantage of maximizing skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to nanoliposomes containing herbal extracts as active ingredients, and include conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has the use of skin anti-aging (skin protection). As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. In addition, skin aging and skin protection are used herein in the same sense unless otherwise specified.

The cosmetic composition of the present invention prevents skin aging through the improvement of skin wrinkles and the improvement of skin elasticity, the moisturizing ability and the skin immunity by promoting the growth of fibroblasts, the promotion of collagen synthesis, such facts are specified in the following examples It is. Thus, skin aging prevention (ie, skin protection), which is a use of the present invention, is construed to include wrinkle improvement, moisturizing improvement and skin elasticity improvement use. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1: Preparation of herbal extract

The herbal extracts were finely divided into 25 g of licorice, 10 g of deer antler, 25 g of goji berry and 10 g of Angelica pulverized by using a crusher, and water was added thereto to make 0.7 L, which was then heated at 70 ° C. for about 4 hours. Subsequently, the mixture was cooled and then cooled at room temperature, and then aged at low temperature at 4 ° C. for 7 days, followed by filtration.

In a preferred embodiment of the present invention, the content of the herbal extract is 0.0001 to 10.0% by weight, more preferably 0.0005 to 10.0% by weight, most preferably 0.005 to 5.0% by weight based on the total cosmetic composition. If the content of the herbal extract is less than 0.0001% by weight, it is not expected to have a clear skin anti-aging effect, in particular, wrinkle improvement. In addition, when the content of the herbal extract exceeds 10.0% by weight, there is no apparent increase in effect as the content increases.

Experimental Example 1 Fibroblast Proliferation Effect

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 1 x 10 ⁴ cells in each well of a 96-well microplate and incubated at 37 for 24 hours in DMEM medium (Sigma). Subsequently, the experimental group was replaced with serum-free DMEM medium containing the final concentration of 0.005%, 0.01%, 0.05%, 0.1% and 1% and the control group replaced with serum-free DMEM medium containing no herbal extract. Was further incubated for 24 hours. Then, 10 μl of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added to each, and incubated for 4 hours. The medium was then removed, shaken for 20 minutes by addition of 100 μl of dimethyl sulfoxide solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). . The measured values were substituted into Equation 1 below to calculate the cell proliferation effect, and the results are shown in Table 1 below.

Cell proliferation effect (%) = [(absorbance at extract treatment-absorbance of control) / absorbance of control] x 100

Extract (%) Absorbance Cell proliferation effect (%) Control 0.330 - 0.005 0.306 5.6 0.001 0.269 18.5 0.05 0.201 39.0 0.1 0.129 60.8 One 0.056 83.1

As can be seen in Table 1, compared with the control group not treated with the herbal extract, the experimental group treated with the herbal extract shows a very high fibroblast proliferation effect.

Experimental Example 2 keratinocyte proliferation effect

Using the keratinocytes to measure the proliferative effect of keratinocytes in the same manner as in Experimental Example 1, the results are shown in Table 2 below.

Extract (%) Absorbance Cell proliferation effect (%) Control 0.324 - 0.005 0.311 4.1 0.001 0.287 11.5 0.05 0.261 19.3 0.1 0.223 31.3 One 0.121 62.8

As shown in Table 2, it can be seen that the herbal extract of the present invention can greatly increase the proliferation of keratinocytes.

Experimental Example 3: Collagen Synthesis Enhancement Effect

Human normal fibroblasts (Korea Cell Line Bank) were inoculated to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated in DMEM medium for 37 to 24 hours. Subsequently, the experimental group in which the prepared herbal extracts were replaced with serum-free DMEM medium containing the final concentrations of 0.005, 0.01 and 0.05% and the control group replaced with serum-free DMEM medium containing no herbal extracts were further added for 24 hours. Cultures were added and ascorbic acid (50 g / ml) was added to promote collagen synthesis and then incubated for another 24 hours. Each well was then washed and replaced with serum-free DMEM medium and incubated for an additional 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a kit (Takara, Japan). PICP amount was converted to ng / 2 × 10 ⁴ cells, and the results are shown in Table 3 below.

Herbal Extract Concentration% (v / v) Collagen Synthesis (ng / 2 x 10 ⁴ ) Control 153 0.005 189 0.01 204 0.05 250

As can be seen in Table 3, it can be seen that the synthesis of collagen increases as the concentration of the herbal extract is increased, and thus the herbal extract of the present invention shows an excellent collagen synthesis enhancing effect.

Experimental Example 4: Skin Immune Enhancement Efficacy Test

After shaving the abdomen in 8-10-week-old C57BL / 6 mice, ultraviolet B wavelength (UVB) of 2.4KJ / m ² using FS-20 sun lamp was used to control the hypersensitivity of the control group. Compared to 44.3% suppressed immunosuppressive phenomenon.

The herbal extract of the present invention was applied after UV irradiation and 3 days later was sensitized with 3% trinitrochlorobenzene in the UV irradiation area. After 6 days, 1% trinitrogen chloride was applied to the ears of mice and edema status was measured. Skin immune enhancing effect was calculated by the following formula, the results are summarized in Table 4 below.

[(Production example application and ear edema thickness of UVB irradiated group-Ear edema thickness of UVB irradiated group) / (Ear edema thickness of control group-Ear edema thickness of UVB irradiated group)] X 100

Herbal Extract Concentration% (v / v) Skin immune enhancing effect (%) Control - 0.005 7.6 0.01 15.4 0.05 33.9

As can be seen in Table 4, it can be seen that the herbal extract of the present invention increases the skin immune response in a concentration-dependent manner.

Preparation Example 5 Preparation of Nanoliposomes Containing Herbal Extracts

The nanoliposomes were added to phase A with the composition of Table 5 below, wetted uniformly, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high-pressure homogenizer 2-4 times at 600-1000 bar and cooled to obtain a nano liposome that stabilized the herbal extract having a particle size of 30-70 nm in diameter.

Prize ingredient Content (% by weight) A Propylene glycol 40 B lecithin 5 cholesterol 2.5 Ceramide 2.5 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Herbal Extract (Solid) 10 Potassium hydroxide Quantity Purified water To 100

Examples and Comparative Examples

Compositions containing nanoliposomes stabilized with the herbal extracts of the present invention (examples) and compositions without nanoliposomes (comparative examples) were prepared in the following compositions.

ingredient Example Comparative example Content (% by weight) Content (% by weight) Nanoliposomes (Table 5) 10 - Herbal Extract (Solid) - 10 A Cetostearyl alcohol 2.0 2.0 Glyceryl Stearate 1.5 1.5 Mikey-Crystalline 0.7 0.7 Squalane 5.0 5.0 Liquid paraffin 3.0 3.0 Trioctanoine 5.0 5.0 Polysorbate 1.2 1.2 Sorbitan stearate 0.5 0.5 Tocopherol Acetate 0.2 0.2 Micromethicone 3.0 3.0 BHT 0.05 0.05 B glycerin 4.0 4.0 1,3-butylene glycol 2.0 2.0 EDTA-2Na 0.05 0.05 Purified water To 100 To 100 Incense, preservative Quantity Quantity

Experimental Example 5: Moisturizing Effect

In order to evaluate the moisturizing power of each cosmetic prepared in the above Examples and Comparative Examples, the moisture retention capacity and the water evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Experimental Example 5-1: Measurement of Water Retention Capacity

In a constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic prepared in Examples and Comparative Examples was coated with a predetermined amount (0.03 g / 16 cm 2) in the inner half of 30 test subjects, and before application, for 1 hour The moisture content of the skin was measured after and 2 hours after application, and normal skin without any treatment was used as a control. As a measuring device, a moisture content meter (corneometer CM820, Conrage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 6 below.

division Example Comparative example Control Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 87 75 50 Skin electrical conductivity 2 hours after application 75 59 50

Experimental Example 5-2: Measurement of Water Evaporation Amount

Tape stripping was applied to the upper arm region of the arm to weaken the skin's protective film. After applying each cosmetic to the subject in the same manner as in Experiment 5-1, the water vaporizer (TEWA meter, koln, Germany) Federated water evaporation was measured 5 times at 1 minute intervals every 12 hours using the Federal Bureau). The results are shown in Table 8 below.

  division Example (%) Comparative example (%) Control group (%) Moisture evaporation after 0 hours 4.0 4.0 0.0 Moisture evaporation after 12 hours -9.1 -5.5 -0.1 Moisture evaporation after 24 hours -7.2 -1.0 0.1 Moisture evaporation after 36 hours -5.0 2.5 0.1 Moisture evaporation after 48 hours -2.3 3.0 0.0

As shown in the results of Experimental Examples 5-1 and 5-2, the cosmetics of the examples of the present invention exhibit higher electrical conductivity of the skin and less water loss than the cosmetics of the comparative examples. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 6: Skin Elasticity Effect

The skin elasticity enhancing effect of the cosmetic containing the nanoliposome stabilized herbal extract of the present invention was measured.

Thirty healthy women (mean age 38 years old) over 20 years old (average age 38 years) were divided into two groups under the conditions of temperature 24-26 ° C and humidity 75. After 12 weeks of application (morning and evening), skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germary). The test results are described as R8 (R8 (12 weeks) -R8 (0 weeks)) values of cutometer SEM 575, where R8 values indicate the nature of the viscoelasticity, and the results are shown in Table 9 below.

Experimental Formulation Skin elasticity effect Example 0.63 Comparative example 0.35

As shown in the results of Table 9, the skin elasticity of the example containing the nanoliposomes stabilized herbal extracts was doubled compared to the comparative example used in direct formulation without stabilizing the herbal extracts. Therefore, it can be seen that the embodiment containing the nanoliposomes stabilized herbal extract shows a much better effect on skin elasticity.

Experimental Example 7: Skin Penetration Effect

The skin penetration effect on the cosmetic containing nanoliposomes stabilized herbal extract of the present invention was measured.

Dermal Equivalent (DE) -human normal fibroblasts 1 X 10 ⁵ cells / mL in a gel structure similar to that of human connective tissue 3 mg / mL: 5 X DEM: 2: 2 sodium bicarbonate and 200 0.05 N sodium hydroxide containing mM Hepes buffer was inoculated in a medium mixed with 7: 2: 1 and incubated at 5CO ₂ , 37 ° C for 7 days. It was placed inside a plate separated from the inside and the outside by a membrane made of a 3 μm porous polycarbonate material, and epidermal Keratinocyte on the surface of Derma Equivalent (DE) where fibroblasts were cultured. ) Inoculated with 1 X 10 ⁵ cell / ㎖ cells and put the K-SFM medium containing EGF and BPE in the inside and outside and incubated for 7 days. Afterwards, the medium inside the plate was discarded to allow the air to come into contact with the surface of the cultured cells. The medium was mixed with K-SFM containing 10FBS and DMEM without EGF in the same amount and incubated for 2 weeks. Artificial skin with dermis was obtained. The experimental and comparative examples of the present invention were applied to the artificial skin tissue of the top layer, and after 4 hours of incubation, the amounts of K-SFM and water containing 10FBS were analyzed by HPLC. The test results are shown in Table 10 below.

Amount of herbal extract in the medium (µg / ml) Example Comparative example 39.5 10.7

Experimental results As shown in Table 10, it can be seen that the cosmetic containing nanoliposomes stabilized herbal extract of the present invention is 10 times more effective in skin penetration than the comparative example using the herbal extract as it is. This is because the average particle size of the nanoliposomes of the present invention is very small, 30-70 nm, so that skin penetration is easy. Therefore, in the above-mentioned skin elasticity enhancing effect of Experimental Example 6, it can be seen that the embodiment of the present invention shows a better skin elasticity enhancing effect because the skin penetration effect is superior to the comparative example.

Experimental Example 8: Stability Test of the Formulation

The stability test for the cosmetic containing the nanoliposome stabilized herbal extract of the present invention was carried out by the following method.

 To test the stability of the cosmetics, the above Examples and Comparative Examples were stored in an opaque glass container in a thermostat kept constant at 45 for 12 weeks and stored in an opaque glass jar in a fully shaded refrigerator kept constant at 4 12 After storage for a week, the degree of separation and discoloration were measured. The results are summarized in Table 11 below. At this time, the degree of product separation and discoloration were evaluated by classifying into the following six grades: 0: no change; 1: very little separation (discoloration); 2: a little separation (discoloration); 3: A little severe separation (discoloration); 4: severely separated (discolored); And 5: very severe separation (discoloration).

Temperature Separation and discoloration Example Comparative example 45 ℃ 0 4.2 4 ℃ 0 0

As can be seen in Table 11, the Example was stable without discoloration or separation at 45, Comparative Example used directly in the formulation without stabilizing the herbal extract is not only unstable extract due to severe separation and discoloration at 45 In addition, it can be seen that the herbal extract entered the formulation and the formulation itself became unstable.

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing a nanoliposome stabilized herbal extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in the following table, the softening lotion was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 1.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrients (Milk Lotion)

Nutrients were prepared according to a conventional method as shown in the table below.

Compounding ingredient Content (% by weight) Nano liposome 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3: Nutrition Cream

Nutritious cream was prepared according to a conventional method as shown in the following table.

Compounding ingredient Content (% by weight) Nano liposome 10.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4: Massage Cream

Massage cream was prepared according to a conventional method as shown in the table below.

Compounding ingredient Content (% by weight) Nano liposome 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5: Pack

Packs were prepared according to conventional methods as shown in the table below.

Compounding ingredient Content (% by weight) Nano liposome 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

 As described above, the present invention provides a cosmetic composition for preventing skin aging comprising a nanoliposome containing the herbal extract as an active ingredient. The cosmetic composition of the present invention prevents skin aging through promoting the growth of fibroblasts, improving skin wrinkles by promoting collagen synthesis and improving skin elasticity, moisturizing and skin immunity. Furthermore, in the cosmetic composition of the present invention, since the herbal extract is contained by the nanoliposomes, the skin penetration effect is greatly increased, and the stability of the formulation is also greatly improved.

Claims (9)

A cosmetic composition for preventing skin aging comprising nanoliposomes stabilized with a mixed extract of licorice, antler, wolfberry and Angelica. The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal extract is contained in an amount of 4-12% by weight based on the total weight of nanoliposomes. According to claim 1, wherein the nanoliposomes are polyols, surfactants, lecithin, cosmetic composition for preventing skin aging, characterized in that the mixture comprising a saturated or unsaturated fatty acid and water of the C ₁₂ -C ₂₂ alkyl chain. The method of claim 3, wherein the polyol is selected from the group consisting of propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, ziitol and sorbitol The content is 30-70% by weight relative to the total weight of the liposomes, the anti-aging cosmetic composition. The method of claim 3, wherein the surfactant is an anionic surfactant or alkoxylated alkyl ether, alkoxylated alkyl ester selected from the group consisting of alkylacylglutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate, A nonionic surfactant selected from the group consisting of alkyl polyglycosides, polyglyceryl esters and sugar esters, the content of which is 0.5-5.0 wt% based on the total weight of nanoliposomes, wherein the cosmetic composition for preventing skin aging. The cosmetic composition for preventing skin aging according to claim 3, wherein the content of lecithin is 2-8% by weight based on the total weight of nanoliposomes. The composition of claim 3, wherein the saturated or unsaturated fatty acid content of the C ₁₂ -C ₂₂ alkyl chain is 0.1-1.0 wt% based on the total weight of the nanoliposomes. The cosmetic composition for preventing skin aging according to claim 3, wherein the nanoliposomes are prepared by applying the mixture to a high pressure homogenizer. The method of claim 1, wherein the liposomes stabilized with the mixed extract of licorice, antler, wolfberry, and Angelica, the skin is characterized in that the action selected from the group consisting of fibroblast growth promoter, collagen biosynthesis promoter, moisturizer and combinations thereof. Anti-aging cosmetic composition.