CN115006308A

CN115006308A - Anti-aging careful essence

Info

Publication number: CN115006308A
Application number: CN202210718185.4A
Authority: CN
Inventors: 冯驯
Original assignee: Megaso Health Industry Guangdong Co ltd
Current assignee: Megaso Health Industry Guangdong Co ltd
Priority date: 2022-06-23
Filing date: 2022-06-23
Publication date: 2022-09-06
Anticipated expiration: 2042-06-23
Also published as: CN115006308B

Abstract

The invention relates to the field of skin care products, and particularly discloses a retrogradation delicate anti-aging essence. The anti-aging careful anti-aging essence comprises the following components in percentage by mass: 3-10% of Chondrus crispus extract; 3-5% of oat kernel oil; 1-5% of Paeonia suffruticosa seed oil; DNA sodium 0.05-0.2%; 0.1-1% of collagen; 0.1-1% of calendula extract; 1-10% of medicinal fomes fomentarius extract; 2-6% of betaine; 0.55 to 1.15 percent of compound sclerotium rolfsii gum; 1-5% of composite polypeptide; 1-4% of emulsifier; 3.4-25% of emollient; 0.1-1% of preservative; the balance being solvent. The anti-aging careful anti-aging essence has the advantages of resisting endogenous aging and exogenous aging simultaneously, and having certain sun-screening and anti-aging effects without additionally using sun-screening cream on skin.

Description

Anti-aging careful essence

Technical Field

The invention relates to the field of skin care products, in particular to an anti-aging essence with a reverse-aging effect.

Background

The historical beauty amateurs have a common final pursuit-youth forever. Therefore, the anti-aging effect is still the most concerned effect of the current skin care products.

Aging is a progressive, irreversible degeneration of physiological structure and function that occurs from the maturation phase of an organism as it ages. Aging of the skin is generally divided into intrinsic aging and extrinsic aging: endogenous aging refers to natural aging occurring with aging, which is mainly due to the degradation of the synthesis capacity of collagen and elastin in the skin with aging and physiological aging, resulting in the deterioration of elastic tissues and the relaxation of the skin; extrinsic aging is mainly photoaging caused by sunlight, and the photoaging is mainly caused by that ultraviolet rays penetrate through the skin, penetrate into tissues below the dermis and destroy the fine structures in the skin such as collagen, elastic fiber tissues and the like, so that wrinkles and color spots are generated on the skin.

However, current anti-aging products generally achieve anti-endogenous aging only, and exogenous aging generally achieves anti-ultraviolet by applying sun block, and thus, there is still room for improvement.

Disclosure of Invention

In order to simultaneously realize endogenous aging resistance and exogenous aging resistance and enable the anti-aging effect to be better, the application provides the anti-aging careful anti-aging essence.

The application provides a retrogressive meticulous anti-aging essence, which adopts the following technical scheme:

the anti-aging careful anti-aging essence comprises the following components in percentage by mass:

3-10% of Chondrus crispus extract;

3-5% of oat kernel oil;

1-5% of Adiantum capillus-veneris seed oil;

DNA sodium 0.05-0.2%;

0.1-1% of collagen;

0.1-1% of calendula extract;

1-10% of medicinal fomes fomentarius extract;

2-6% of betaine;

0.55 to 1.15 percent of compound sclerotium rolfsii gum;

1-5% of compound polypeptide;

1-4% of emulsifier;

3.4-25% of emollient;

0.1-1% of preservative;

the balance being solvent.

The Chondrus crispus extract is a water-locking and moisture-keeping raw material of cosmetics extracted from natural seaweed, can promote the differentiation of skin keratinocytes, and is beneficial to increasing the amount of protein and oxygen required by a protein membrane structure on the surface of skin, so that the formation of a membrane structure is promoted, the skin moisture is increased, and the phenomenon of rough skin is relieved.

The oat kernel oil is oil refined from oat kernels, can naturally moisten skin, is suitable for sensitive and red skin, and has an anti-irritation effect.

The Papaveris seed oil is a high-grade oil-based material, and has good skin softening effect and antioxidant effect.

The DNA sodium exists in human placenta, is one of complexes of cell-generated DNA raw materials, is derived from the controlled purification and sterilization process of protamine DNA, has special capability of promoting recovery after skin transplantation, and can promote human cell regeneration.

Collagen is the most prominent water-insoluble fibrin in the extracellular matrix and can form a similar fibrous structure.

The main active ingredient of the calendula is a carotenoid compound, the content of the carotenoid compound accounts for about 3 percent of the dry flower, the calendula mainly contains lutein, zeaxanthin, carotene, a large amount of lutein esters and the like, and the calendula extract has the functions of resisting inflammation and bacteria and particularly has good effect on staphylococcus and streptococcus; the calendula extract also has a proliferation effect on epidermal cells and dermal cells, has excellent antioxidant property, can enhance skin activity, and is favorable for better enhancing the anti-aging effect to a certain extent.

The medicinal fomes fomentarius extract is a pure concentrated active substance extracted from basidiomycetes-fomes fomentarius meat juice growing in eastern Europe, can solve excessive secretion of skin oil, and has effects of astringing, shrinking pores, and smoothing and tightening skin.

Through the synergistic compounding of the components in a specific proportion, the betaine is favorable for better promoting DNA sodium, collagen, the calendula extract and the fomes officinalis extract to deeply permeate into the skin and reach tissues below the dermis of the skin to play a role, so that various growth factors rich in the components can stimulate the generation of a large amount of collagen, elastic fibers, colloid and the like in the dermis and the tissues below the dermis, and the aim of removing wrinkles is fulfilled; meanwhile, the substances are also beneficial to activating the synthesis of mucopolysaccharide, promoting the synthesis of collagen, increasing the number of collagen fibers, so that the epidermis layer and the dermis layer of the skin are firmer and tighter, the skin becomes fuller and fuller, and the skin wrinkles are fewer; in addition, the emollient is also favorable for better penetrating to the dermis layer of the skin to realize deep water supplement under the penetration promoting effect of betaine, the oat kernel oil and the monkey-bread tree seed oil are also favorable for forming a protective film on the surface layer of the skin in cooperation, the oat kernel oil and the monkey-bread tree seed oil are favorable for better locking skin moisture and preventing the loss of the skin moisture, and the formed protective film is also favorable for better resisting the damage of ultraviolet rays to the skin, so that the essence can simultaneously realize endogenous aging resistance and exogenous aging resistance, and the skin can also play a certain sun-screening and aging-resisting effect without additionally using sun-screening cream.

Preferably, the compound sclerotium rolfsii glue consists of the following components in percentage by mass:

32-36% of hydrolyzed sclerotium rolfsii gum;

28-32% of xanthan gum;

32-36% of Chondrus crispus extract.

The compound sclerotium rolfsii gum is formed by adopting the hydrolyzed sclerotium rolfsii gum, the xanthan gum and the carrageen crispus extract in a specific proportion in a synergistic manner, so that the consistency of the essence can be better adjusted, the stability of the essence can be better improved, and the essence can form a protective film on the surface layer of the skin, so that the damage of ultraviolet rays to the skin can be better resisted, and the sun-proof and anti-aging effects of the essence can be better improved.

Preferably, the composite polypeptide consists of the following components in percentage by mass:

palmitoyl tripeptide-50.01-0.03%;

70.01 to 0.02 percent of palmitoyl tetrapeptide;

acetyl tetrapeptide-90.02-0.04%;

palmitoyl pentapeptide-40.01-0.02%;

hexapeptide-90.01-0.05%;

palmitoyl hexapeptide-120.003-0.008%;

0.1-0.5% of tetrahydro-methyl-pyrimidine carboxylic acid;

1-5% of PEG-40 hydrogenated castor oil;

25-32% of butanediol;

3-8% of 1, 2-hexanediol;

3-8% of 1, 2-pentanediol;

the balance being water.

Through synergistic compounding of the substances in a specific ratio, the PEG-40 hydrogenated castor oil is beneficial to better improving the solubility of each component, and meanwhile, the composite polypeptide is also beneficial to better compatibility and synergy with other components in the essence, such as an emollient, a preservative and the like, so that each component can better exert the effect; meanwhile, the specific peptides are compounded in a synergistic manner according to a specific proportion, so that multiple nursing can be formed on the skin, and a skin barrier can be formed, so that the damage of ultraviolet rays to the skin can be better resisted, the loss of skin collagen can be better reduced, and the sun-screening and anti-aging effects of the essence can be better.

Preferably, the anti-aging careful anti-aging essence further comprises the following components in percentage by mass:

0.5-1% of tremella polysaccharide;

0.1-1% of ergothioneine;

0.1 to 1 percent of vitreous origin.

The tremella polysaccharide is prepared by deep culture of tremella fuciformis of Basidiomycetes, is a polysaccharide consisting of acidic heteropolysaccharide, has certain capability of removing hydroxyl free radicals, and is beneficial to improving the anti-aging effect of the essence.

Ergothioneine can reduce the formation of active oxygen, and is also favorable for protecting skin cells from radiation damage, so that when ultraviolet rays in sunlight permeate into the dermis of human skin, the growth of the epidermal cells is less susceptible, the epidermal cells are less susceptible to aging, the skin photoaging is not easily caused, and better exogenous aging resistance is facilitated.

The vitreous color is beneficial to better promoting the generation of elastin, so that the quantity of collagen and collagen fibers of the skin is increased, the skin is better compacted, and wrinkles are reduced.

By adding the tremella polysaccharide, the ergothioneine and the vitriol gene to be compounded in a synergistic manner, the adverse-age effect can be better realized, the quantity of collagen and collagen fibers of the skin can be continuously increased, and the skin wrinkles can be continuously relieved.

2-6% of nicotinamide.

By adding the nicotinamide, the whitening effect of the essence is improved better, the skin is less prone to generating color spots or dark skin color and the like due to ultraviolet ray damage, the essence can resist exogenous aging at the same time, and sunscreen cream is not needed to be additionally used.

Preferably, the emulsifier consists of cetearyl alcohol and cetearyl glucoside in a mass ratio of 4-5: 1.

The cetearyl alcohol and the cetearyl glucoside in a specific ratio are cooperatively compounded to serve as an emulsifier, so that the stability of an essence system is favorably improved, the compatibility of each component is better, and the components are favorably and better exert a synergistic effect.

Preferably, the emollient comprises one or more of 1, 3-propanediol, hydrogenated polyisobutene, squalane, 1, 2-hexanediol, 1, 2-pentanediol.

Preferably, the emollient is composed of the following components in percentage by mass of the essence:

1-10% of 1, 3-propylene glycol;

1-5% of hydrogenated polyisobutene;

0.3 to 2 percent of squalane;

0.1-3% of 1, 2-hexanediol;

1-5% of 1, 2-pentanediol.

Through adopting the substances with specific proportions to cooperate as the emollient, the deep water replenishing effect of the essence can be better improved, the water content of the skin is higher, the skin can be always kept in a full state, and the skin can be younger.

Preferably, the preservative consists of phenoxyethanol and ethylhexyl glycerol in a mass ratio of 9: 1.

The phenoxyethanol and the ethylhexyl glycerin in a specific ratio are used as the preservative in a synergistic manner, so that the stability of the essence can be better improved, and the shelf life of the essence can be better prolonged; meanwhile, the skin is not easy to be irritated, and the mildness of the essence can be better kept.

In summary, the present application has the following beneficial effects:

1. the betaine is favorable for better promoting the DNA sodium, the collagen, the calendula extract and the officinal porus fungus extract to deeply permeate the skin and reach tissues below the dermis of the skin to play a role, so that various growth factors rich in the components can stimulate the generation of a large amount of collagen, elastic fibers, colloid and the like on the dermis and the tissues below the dermis, and the aim of removing wrinkles is fulfilled.

2. The specific proportion of the betaine, the DNA sodium, the collagen, the calendula extract and the medicinal porifera extract is also beneficial to activating the synthesis of mucopolysaccharide, promoting the synthesis of collagen, increasing the number of collagen fibers, enabling the epidermal layer and the dermal layer of the skin to be firmer and tighter, enabling the skin to become fuller and enabling the skin to have fewer wrinkles.

3. The skin moisturizer is also favorable for better permeating to the dermis layer of the skin to realize deep water replenishing under the permeation promoting effect of betaine, the combination of the carrageen crispatus extract, the oat kernel oil and the Adiantum montanum seed oil is also favorable for forming a protective film on the surface layer of the skin, the skin moisture is favorably locked and prevented from losing, the formed protective film is also favorable for better resisting the damage of ultraviolet rays to the skin, the essence can simultaneously realize endogenous aging resistance and exogenous aging resistance, and the skin can also play a certain sun-screening and aging-resisting effect without additionally using sun-screening cream.

4. By adopting the specific polypeptides in a specific proportion to be compounded in a synergistic manner, multiple nursing can be formed on the skin, and a skin barrier can be formed, so that the damage of ultraviolet rays to the skin can be better resisted, the loss of skin collagen can be better reduced, and the sun-screening and anti-aging effects of the essence can be better.

5. By adding the tremella polysaccharide, the ergothioneine and the vitriol gene to be compounded in a synergistic manner, the adverse-age effect can be better realized, the quantity of collagen and collagen fibers of the skin can be continuously increased, and the skin wrinkles can be continuously relieved.

Drawings

Fig. 1 is a comparison graph of pre-and post-use effects on skin darkening before and 30 days after serum use in a 37 year old volunteer in experiment 2.

Fig. 2 is a comparison graph of pre-and post-use effects on skin darkening in a 40 year old volunteer before and 30 days after serum use in experiment 2.

Fig. 3 is a comparison graph of the darkness of the skin of a 45-year-old volunteer before and 30 days after the serum was administered in experiment 2.

Fig. 4 is a comparison graph of the wrinkle-changing effect of a 39 year old volunteer in experiment 2 before, 14 days after and 30 days after the serum application.

Fig. 5 is a comparison chart of the wrinkle-changing effect of a 47 year old volunteer before, 14 days after and 30 days after the serum application in experiment 2.

Wherein, the left images in figures 1-3 are the effect images before using the essence, and the right images are the effect images after using the essence for 30 days; in fig. 4-5, the left graph is the effect graph before the essence is used, the middle graph is the effect graph 14 days after the essence is used, and the right graph is the effect graph 30 days after the essence is used.

Detailed Description

The present application will be described in further detail with reference to examples.

In the following examples and comparative examples, each raw material was commercially available. Specifically, Chondrus crispus extract was obtained from Pasteur (China) Ltd; oat kernel oil was purchased from Fazer Finland Oy, Fazer Mills; macadamia seed oil was purchased from Kupanda Limited; collagen was purchased from Shenzhen Yinnuosi biomedicine GmbH; the calendula extract was purchased from Xian Lutian Biotechnology Ltd; the fomes officinalis extract for pharmaceutical use is available from basf (china) limited. The above plant extracts are all obtained by alcohol extraction, and the extraction solvent is butanediol.

Preparation example 1

The preparation example discloses a composite sclerotium rolfsii gum, which consists of the following components in parts by mass:

640g of hydrolyzed sclerotium rolfsii glue; 640g of xanthan gum; 720g of Chondrus crispus extract.

The preparation example also discloses a preparation method of the composite sclerotium rolfsii gum, which comprises the following specific steps: accurately weighing the components according to the mass, and stirring and mixing the components uniformly at normal temperature to obtain the compound sclerotium rolfsii gum.

Preparation example 2

The difference from preparation example 1 is that:

the dosage of each component is different, and the specific dosage is as follows:

hydrolyzing sclerotium rolfsii gum 720 g; 560g of xanthan gum; 720g of Chondrus crispus extract.

Preparation example 3

The difference from preparation example 1 is that:

hydrolyzing sclerotium rolfsii gum 720 g; 640g of xanthan gum; 640g of Chondrus crispus extract.

Preparation example 4

The preparation example discloses a composite polypeptide, which consists of the following components in parts by mass:

palmitoyl tripeptide-51 g; palmitoyl tetrapeptide-71 g; acetyl tetrapeptide-92 g; palmitoyl pentapeptide-41 g; hexapeptide-91 g; palmitoyl hexapeptide-120.3 g; 10g of tetrahydro-methyl-pyrimidine-carboxylic acid; 100g of PEG-40 hydrogenated castor oil; 2500g of butanediol; 300g of 1, 2-hexanediol; 300g of 1, 2-pentanediol; 6783.7g of water.

The preparation example also discloses a preparation method of the composite polypeptide, which comprises the following steps: accurately weighing the components according to the mass, dissolving palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, hexapeptide-9 and palmitoyl hexapeptide-12 in a mixed solution of PEG-40 hydrogenated castor oil, butanediol, 1, 2-hexanediol, 1, 2-pentanediol and water, adding tetrahydro-methyl pyrimidine carboxylic acid, and stirring uniformly to obtain the composite polypeptide.

Preparation example 5

The difference from preparation example 4 is that:

palmitoyl tripeptide-53 g; palmitoyl tetrapeptide-72 g; acetyl tetrapeptide-94 g; 42 g of palmitoyl pentapeptide; hexapeptide-95 g; 120.8 g of palmitoyl hexapeptide; 50g of tetrahydro-methyl-pyrimidine-carboxylic acid; 500g of PEG-40 hydrogenated castor oil; 3200g of butanediol; 800g of 1, 2-hexanediol; 800g of 1, 2-pentanediol; 4633.2g of water.

Preparation example 6

The difference from preparation example 4 is that:

palmitoyl tripeptide-52 g; palmitoyl tetrapeptide-71.5 g; acetyl tetrapeptide-93 g; palmitoyl pentapeptide-41.5 g; hexapeptide-92.5 g; palmitoyl hexapeptide-125 g; 30g of tetrahydro-methyl-pyrimidine-carboxylic acid; 300g of PEG-40 hydrogenated castor oil; 3000g of butanediol; 500g of 1, 2-hexanediol; 500g of 1, 2-pentanediol; 5654.5g of water.

Preparation example 7

The difference from preparation example 4 is that: the palmitoyl tripeptide-5 is replaced by an equal amount of palmitoyl tripeptide-1.

Preparation example 8

The difference from preparation example 4 is that: the palmitoyl tetrapeptide-7 was replaced with an equal amount of palmitoyl tetrapeptide-3.

Preparation example 9

The difference from preparation example 4 is that: acetyl tetrapeptide-9 was replaced with an equal amount of palmitoyl acetyl tetrapeptide-4.

Preparation example 10

The difference from preparation example 4 is that: the hexapeptide-9 was replaced with an equal amount of hexapeptide-1.

Preparation example 11

The difference from preparation example 4 is that: the palmitoyl hexapeptide-12 was replaced by an equal amount of palmitoyl hexapeptide-8.

Preparation example 12

The difference from preparation example 4 is that:

palmitoyl tripeptide-54 g; palmitoyl tetrapeptide-73 g; acetyl tetrapeptide-91 g; palmitoyl pentapeptide-40.5 g; hexapeptide-96 g; palmitoyl hexapeptide-120.1 g; 10g of tetrahydro-methyl-pyrimidine-carboxylic acid; 100g of PEG-40 hydrogenated castor oil; 2500g of butanediol; 300g of 1, 2-hexanediol; 300g of 1, 2-pentanediol; 6775.4g of water.

Example 1

The embodiment discloses an anti-aging careful anti-aging essence which comprises the following components in parts by mass:

3kg of Chondrus crispus extract; 3kg of oat kernel oil; 1kg of Paeonia suffruticosa seed oil; 0.05kg of DNA sodium; 0.1kg of collagen; 0.1kg of calendula extract; 1kg of medicinal fomes fomentarius extract; 2kg of betaine; 0.55kg of compound sclerotium rolfsii gum; 1kg of composite polypeptide; 1kg of emulsifier; 3.4kg of emollient; 0.1kg of preservative; 83.7kg of water.

In this example, the compound sclerotium rolfsii gum prepared in preparation example 1 was used; composite polypeptide the composite polypeptide prepared in preparation example 4 was used; the emulsifier consists of 0.8kg of cetearyl alcohol and 0.2kg of cetearyl glucoside; the emollient consists of 1kg of 1, 3-propanediol, 1kg of hydrogenated polyisobutene, 0.3kg of squalane, 0.1kg of 1, 2-hexanediol and 1kg of 1, 2-pentanediol; the preservative consisted of 0.09kg phenoxyethanol and 0.01kg ethylhexyl glycerol.

The embodiment also discloses a preparation method of the inverse-age meticulous anti-aging essence, which comprises the following steps of:

step 1, cleaning and sterilizing production equipment, and accurately weighing the components according to the mass.

And 2, sequentially adding water, compound sclerotium rolfsii gum, a carrageen crispus extract, 1, 3-propylene glycol and betaine into a water phase pot, raising the temperature to 85 ℃, stirring at the rotating speed of 2000r/min, homogenizing for 3min, and preserving the temperature for 15min to obtain the phase A composition.

And 3, adding cetearyl alcohol, cetearyl glucoside, oat kernel oil, Pausia japonica seed oil, hydrogenated polyisobutene and squalane into an oil phase pot in sequence, raising the temperature to 85 ℃, stirring at the rotating speed of 3000r/min, and keeping the temperature for 5min to obtain the B-phase composition.

And 4, slowly pumping the B-phase composition into the A-phase composition, homogenizing and stirring at the rotating speed of 2500r/min for 8min, stopping homogenizing, continuing to keep the temperature and stir for 30min, vacuumizing to the vacuum degree of 0.4MPa, removing foams in the system, and recovering to the normal pressure after the foams are completely removed.

And 5, stopping heating, reducing the temperature under natural conditions, sequentially adding the DNA sodium, the composite polypeptide, the collagen, the calendula extract and the fomes officinalis extract when the temperature is reduced to 45 ℃, continuously stirring uniformly to obtain a C-phase composition, sequentially adding the phenoxyethanol, the ethylhexyl glycerol, the 1, 2-hexanediol and the 1, 2-pentanediol when the temperature is continuously reduced to 40 ℃, continuously stirring for 20min, and stopping stirring to obtain a semi-finished product.

And 6, filtering the semi-finished product through a 100-mesh filter cloth, discharging, and standing the discharged semi-finished product for 12 hours to obtain the anti-aging delicate anti-aging essence.

Example 2

The difference from example 1 is that:

10kg of Chondrus crispus extract; 5kg of oat kernel oil; 5kg of Paeonia suffruticosa seed oil; 0.2kg of DNA sodium; 1kg of collagen; 1kg of calendula extract; 10kg of medicinal fomes fomentarius extract; 6kg of betaine; 1.15kg of compound sclerotium rolfsii gum; 5kg of composite polypeptide; 4kg of emulsifier; 25kg of emollient; 1kg of preservative; 25.65kg of water.

In this example, the compound sclerotium rolfsii gum prepared in preparation example 2 was used; composite polypeptide the composite polypeptide prepared in preparation 5 is adopted; the emulsifier consists of 3.2kg cetearyl alcohol and 0.8kg cetearyl glucoside; the emollient consists of 10kg of 1, 3-propanediol, 5kg of hydrogenated polyisobutene, 2kg of squalane, 3kg of 1, 2-hexanediol and 5kg of 1, 2-pentanediol; the preservative consisted of 0.9kg phenoxyethanol and 0.1kg ethylhexyl glycerol.

Example 3

The difference from example 1 is that:

5kg of Chondrus crispus extract; 4kg of oat kernel oil; 3kg of Paeonia suffruticosa seed oil; 0.1kg of DNA sodium; 0.5kg of collagen; 0.5kg of calendula extract; 5kg of medicinal fomes fomentarius extract; 4kg of betaine; 1kg of compound sclerotium rolfsii gum; 3kg of composite polypeptide; 3kg of emulsifier; 14kg of emollient; 0.5kg of preservative; 56.4kg of water.

In this example, the compound sclerotium rolfsii gum prepared in preparation example 3 was used; the composite polypeptide prepared in preparation example 6 is adopted; the emulsifier consists of 2.5kg cetearyl alcohol and 0.5kg cetearyl glucoside; the emollient consisted of 5kg of 1, 3-propanediol, 3kg of hydrogenated polyisobutene, 1kg of squalane, 2kg of 1, 2-hexanediol and 3kg of 1, 2-pentanediol; the preservative consisted of 0.45kg phenoxyethanol and 0.05kg ethylhexyl glycerol.

Example 4

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 7 was used.

Example 5

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 8 was used.

Example 6

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 9 was used.

Example 7

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 10 was used.

Example 8

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 11 was used.

Example 9

The difference from example 3 is that: composite polypeptide the composite polypeptide prepared in preparation example 12 was used.

Example 10

The difference from example 3 is that:

the anti-aging careful anti-aging essence also comprises the following components in parts by mass:

0.5kg of tremella polysaccharide; 0.1kg of ergothioneine; 0.1kg of vitreous enamel. The amount of water added was adjusted to 55.7 kg.

Wherein, the tremella polysaccharide is added in step 2 to form a phase A composition; ergothioneine and vitronectin were both added after the fomes officinalis extract in step 5 to form a phase C composition.

Example 11

The difference from example 3 is that:

1kg of tremella polysaccharide; 1kg of ergothioneine; bobei powder is 1 kg. The amount of water added was adjusted to 53.4 kg.

Wherein, tremella polysaccharide is added in step 2 to form a phase A composition; ergothioneine and vitronectin were both added after the fomes officinalis extract in step 5 to form a phase C composition.

Example 12

The differences from example 10 are: equal amount of ergothioneine is used for replacing tremella polysaccharide.

Example 13

The difference from example 10 is that: equal amount of vitronectin was substituted for ergothioneine.

Example 14

The difference from example 10 is that: the same amount of tremella polysaccharide is used for replacing the vitreosine.

Example 15

The difference from example 3 is that:

0.8kg of tremella polysaccharide; 0.5kg of ergothioneine; 0.5kg of vitreous opal; 2kg of nicotinamide. The amount of water added was adjusted to 52.6 kg.

Comparative example 1

The difference from example 3 is that: equal amount of water is used to replace betaine, DNA sodium, collagen, calendula extract and medicinal fungus extract.

Comparative example 2

The difference from example 3 is that: equal amounts of water were substituted for betaine.

Comparative example 3

The difference from example 3 is that: equal amounts of water were used instead of sodium DNA.

Comparative example 4

The difference from example 3 is that: equal amounts of water were substituted for the collagen.

Comparative example 5

The difference from example 3 is that: equal amounts of water were substituted for the marigold extract.

Comparative example 6

The difference from example 3 is that: the medicinal fomes fomentarius extract was replaced with an equal amount of water.

Comparative example 7

The difference from example 3 is that: equal amount of water was substituted for oat kernel oil and monkey bushy seed oil.

Comparative example 8

The difference from example 3 is that: equal amounts of water were substituted for oat kernel oil.

Comparative example 9

The difference from example 3 is that: equal amount of water was substituted for the Adiantum montanum seed oil.

Comparative example 10

Commercially available essence.

Experiment 1

20 μ L of each of the essences prepared in the above examples and comparative examples was taken and added to 1.98mL of 10% FBS-DMEM medium to prepare sample solutions, which were then diluted with 10% FBS-DMEM medium to 0.001% by mass concentration in each of sample solution 1 (corresponding to example 1), sample solution 2 (corresponding to example 2), sample solution 3 (corresponding to example 3), sample solution 4 (corresponding to example 4), sample solution 5 (corresponding to example 5), sample solution 6 (corresponding to example 6), sample solution 7 (corresponding to example 7), sample solution 8 (corresponding to example 8), sample solution 9 (corresponding to example 9), sample solution 10 (corresponding to example 10), sample solution 11 (corresponding to example 11), sample solution 12 (corresponding to example 12), sample solution 13 (corresponding to example 13), and, Sample solution 14 (corresponding to example 14), sample solution 15 (corresponding to example 15), control solution 1 (corresponding to comparative example 1), control solution 2 (corresponding to comparative example 2), control solution 3 (corresponding to comparative example 3), control solution 4 (corresponding to comparative example 4), control solution 5 (corresponding to comparative example 5), control solution 6 (corresponding to comparative example 6), control solution 7 (corresponding to comparative example 7), control solution 8 (corresponding to comparative example 8), control solution 9 (corresponding to comparative example 9), and control solution 10 (corresponding to comparative example 10).

Human fibroblasts were plated in 96-well plates at a density of 1 ten thousand per well and cultured in an incubator for 24 hours. Discarding the supernatant, adding the sample solution 1, sample solution 2, sample solution 3, sample solution 4, sample solution 5, sample solution 6, sample solution 7, sample solution 8, sample solution 9, sample solution 10, sample solution 11, sample solution 12, sample solution 13, sample solution 14, sample solution 15, control solution 1, control solution 2, control solution 3, control solution 4, control solution 5, control solution 6, control solution 7, control solution 8, control solution 9, and control solution 10 prepared as described above to different well plates, adding 200. mu.L of each well, taking a tube without any substance as a blank control, incubating in an incubator for 48 hours, diluting 100. mu.L of the supernatant with DMEM medium 10 times, and quantifying collagen I in the supernatant using Human ColI Elisa Kit, recording as the conventional collagen content (ng/mL); the remaining supernatant was irradiated with ultraviolet light (UVB) for 10min, cultured for 6h, collected, diluted 10-fold with DMEM medium, and the amount of collagen i in the supernatant was determined using Human Col i Elisa Kit and recorded as the solarized collagen content (ng/mL).

Experiment 2

250 female volunteers of 30-50 years old are randomly selected, and are randomly divided into 25 groups, and the essence prepared by the above embodiment and the comparative example is respectively and correspondingly applied to the face, and is used twice a day, once in the morning and evening, and continuously used for 30 days. And volunteers were tested as follows:

detection 1

Before the volunteers use the essence, a skin moisture tester CM825 is adopted to detect the skin moisture content of the volunteers respectively, and the average value of the skin moisture content of the same group of volunteers is calculated and recorded as the skin moisture content before use. And after the volunteers use the essence for the first time, after the volunteers use the essence for 3h, 8h and 12h, respectively detecting the skin moisture content of the volunteers again by using a skin moisture meter CM825, calculating and recording the average value of the skin moisture content measured by the same group of volunteers, and respectively recording the average value as the skin moisture content after 3h use, the skin moisture content after 8h use and the skin moisture content after 12h use.

The higher the detected skin moisture content is, the better the moisturizing effect of the essence is.

Detection 2

Before the volunteers use the essence, detecting the facial skin tightness of the volunteers by using a skin elasticity tester MPA580, and calculating the average value of the skin tightness of the same group of volunteers, and recording the average value as the skin tightness before use; and after the volunteers use the essence for 30 days, detecting the facial skin tightness of the volunteers by using a skin elasticity tester MPA580 again, calculating the average value of the skin tightness of the same group of volunteers, recording the average value as the skin tightness after use, and calculating the change rate (%) of the skin tightness before and after the volunteers use the essence. Wherein the content of the first and second substances,

the lower the skin firmness obtained by detection, the better the skin firmness is indicated.

Detection 3

Before the volunteers use the essence, firstly, detecting facial skin wrinkles of the volunteers by using a skin rapid three-dimensional imaging system PRIMOS, calculating the average value of the skin wrinkles measured by the same group of volunteers, and recording the average value as the skin wrinkles before use; and after the volunteers use the facial skin wrinkles for 30 days, detecting the facial skin wrinkles of the volunteers by using a skin rapid three-dimensional imaging system PRIMOS again, calculating the average value of the skin wrinkles measured by the same group of volunteers, recording the average value as the skin wrinkles after use, and calculating the change rate (%) of the skin wrinkles before and after the volunteers use the essence. Wherein the content of the first and second substances,

the lower the skin wrinkles detected, the less wrinkles the skin is detected, and the more youthful the skin is.

The data obtained from the above experiments are shown in tables 1 and 2.

TABLE 1

TABLE 2

According to the data comparison between the embodiment 3 in the tables 1 and 2 and the comparative examples 1 to 6, the synergistic combination of the betaine, the DNA sodium, the collagen, the calendula extract and the medicinal fungus extract in a specific ratio is beneficial to the substances to better permeate to the deep layer of the skin under the action of the betaine, so that the substances are beneficial to better and deeply moisturizing, and the substances are beneficial to better promoting the generation of collagen, so that the number of collagen and collagen fibers is increased, further the anti-aging effect of wrinkle reduction and skin tightening is realized, and any substance is lacked, so that the anti-ultraviolet effect is easily influenced to a certain extent, the content of the collagen is influenced, and the effects of wrinkle reduction of essence and skin tightening are further influenced.

According to the data comparison between the example 3 and the comparative examples 7-9 in the tables 1 and 2, the oat kernel oil and the monkey bushy seed oil are added for synergistic compounding, so that the protective barrier can be better formed on the surface of the skin, the long-acting moisturizing effect can be better facilitated, the skin can be better resisted by ultraviolet rays, the content of collagen can be less affected by the ultraviolet rays, the essence can be better used for reducing wrinkles and firming the skin, and the barrier protection effect cannot be achieved due to the absence of any substance.

As can be seen from comparison of the data of example 3 and comparative example 10 in tables 1 and 2, although the existing commercially available essence can achieve an anti-aging effect, the existing essence does not have an effect of resisting ultraviolet rays, the growth of collagen is easily affected when the collagen is attacked by ultraviolet rays, and the existing essence only has an effect of delaying the generation of wrinkles, and thus the effect of smoothing the generated wrinkles is difficult to achieve.

According to the comparison of the data of the examples 3-9 in the tables 1 and 2, the effect of better resisting the ultraviolet ray to the skin can be favorably realized only by adopting the specific peptides with specific proportions to be cooperatively compounded as the composite polypeptide, and the effect of resisting the ultraviolet ray can be easily influenced by replacing any polypeptide or changing the dosage proportion among substances, so that the content of the collagen can be easily influenced.

According to the data comparison between the example 3 and the examples 10-14 in the tables 1 and 2, the tremella polysaccharide, the ergothioneine and the vitriol are compounded in a specific ratio to promote the generation of collagen better and smooth the generated wrinkles better, and any substance is absent, so that the effect of delaying the generation of the wrinkles can be achieved, and the effect of smoothing the generated wrinkles is difficult to achieve.

Referring to fig. 1-3, after the volunteer uses the essence for 30 days, the dark skin color is obviously improved, which means that the essence has a certain ultraviolet ray resistance effect, so that the essence can also have a sunscreen effect to a certain extent without additionally applying sunscreen cream.

Referring to fig. 4-5, the wrinkles of the volunteers are obviously reduced after the volunteers use the essence for 14 days and 30 days, which indicates that the essence of the present application has anti-aging wrinkle removing and tightening effects.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. The anti-aging careful essence is characterized in that: the paint comprises the following components in percentage by mass:

3-10% of Chondrus crispus extract;

3-5% of oat kernel oil;

1-5% of Paeonia suffruticosa seed oil;

0.05 to 0.2 percent of DNA sodium;

0.1-1% of collagen;

0.1-1% of calendula extract;

1-10% of medicinal fomes fomentarius extract;

2-6% of betaine;

0.55 to 1.15 percent of compound sclerotium rolfsii gum;

1-5% of compound polypeptide;

1-4% of an emulsifier;

3.4-25% of emollient;

0.1-1% of preservative;

the balance being solvent.

2. The essence according to claim 1, wherein the essence is prepared from the following components: the compound sclerotium rolfsii gum comprises the following components in percentage by mass:

32-36% of hydrolyzed sclerotium rolfsii gum;

28-32% of xanthan gum;

32-36% of Chondrus crispus extract.

3. The retrogradation fine anti-aging essence as claimed in claim 1, wherein: the composite polypeptide comprises the following components in percentage by mass:

palmitoyl tripeptide-50.01-0.03%;

70.01 to 0.02 percent of palmitoyl tetrapeptide;

acetyl tetrapeptide-90.02-0.04%;

40.01 to 0.02 percent of palmitoyl pentapeptide;

hexapeptide-90.01-0.05%;

palmitoyl hexapeptide-120.003-0.008%;

0.1-0.5% of tetrahydro-methyl-pyrimidine carboxylic acid;

1-5% of PEG-40 hydrogenated castor oil;

25-32% of butanediol;

3-8% of 1, 2-hexanediol;

3-8% of 1, 2-pentanediol;

the balance being water.

4. The essence for resisting aging according to any one of claims 1 to 3, wherein: the anti-aging careful anti-aging essence also comprises the following components in percentage by mass:

0.5-1% of tremella polysaccharide;

0.1-1% of ergothioneine;

0.1 to 1 percent of vitreous origin.

5. The retrogradation fine anti-aging essence as claimed in any one of claims 1 to 3, wherein: the anti-aging careful anti-aging essence also comprises the following components in percentage by mass:

2-6% of nicotinamide.

6. The retrogradation fine anti-aging essence as claimed in any one of claims 1 to 3, wherein: the emulsifier consists of cetearyl alcohol and cetearyl glucoside in a mass ratio of 4-5: 1.

7. The retrogradation fine anti-aging essence as claimed in any one of claims 1 to 3, wherein: the emollient comprises one or more of 1, 3-propylene glycol, hydrogenated polyisobutene, squalane, 1, 2-hexanediol, and 1, 2-pentanediol.

8. The essence of claim 7, wherein the essence comprises: the emollient is composed of the following components in percentage by mass of the essence:

1-10% of 1, 3-propylene glycol;

1-5% of hydrogenated polyisobutene;

0.3 to 2 percent of squalane;

0.1-3% of 1, 2-hexanediol;

1-5% of 1, 2-pentanediol.

9. The retrogradation fine anti-aging essence as claimed in any one of claims 1 to 3, wherein: the preservative consists of phenoxyethanol and ethylhexyl glycerin in a mass ratio of 9: 1.