CN110731928A - Anti-aging cosmetic composition - Google Patents
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The present invention relates to kinds of concentrated birch sap having a significant anti-aging effect at a concentration of about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times, and an anti-aging cosmetic composition comprising the concentrated birch sap, which shows a significant anti-aging effect.
Description
Technical Field
The present invention relates to kinds of concentrated birch sap, and an anti-aging cosmetic composition comprising the same.
Background
Birch is a deciduous tree of the betulinaceae family, and currently, there are about 100 varieties worldwide, mainly distributed in the northern temperate zone and the cold temperate zone. Wherein, there are about 29 varieties in China, and the varieties are mainly distributed in the northeast, northwest and southwest. Birch trees are mostly grown in remote mountainous areas with less human intervention and no industrial pollution. Birch sap (also called birch sap) is fresh sap obtained by cutting bark or drilling trunk of birch, and is colorless or yellowish, has no precipitate or impurity, and has light birch fragrance. The birch juice contains abundant saccharide, amino acids, vitamins, biotin, cytokinin, trace mineral elements, aromatic oil, betulin, saponin, etc., and has good antiaging effect.
Skin aging refers to the deterioration of the protective ability and the regulating ability of the skin to the body, the inability to adapt to the change of internal and external environments, the appearance of the whole change of color, shape and the like, and is often manifested by the reduction or disappearance of the elasticity of the skin, the slack skin, the increase of wrinkles, the dryness and the thinning of the skin, easy desquamation, pruritus, dull complexion and no luster, and the influence on the appearance and self-confidence along with color spots and warts. With the increasing population aging problems and the increasing living standard, consumers' awareness of anti-wrinkle is increasing, and more consumers are eager to use cosmetics to maintain a youthful appearance. According to statistics, the anti-aging cosmetics become the category with the fastest growth speed and the largest potential in the Chinese cosmetic market.
Disclosure of Invention
In aspect, the present invention provides concentrated birch juices with a concentration factor of about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times.
Birch juices involved in the present invention are obtained from Betula genus of betulinaceae family, which may be from four varieties of white birch (Betula alba), Betula luminifera (Betula pubescens), Betula pendula (Betula pendula) and birch asiana (Betula platyphylla). The birch juice is colorless, transparent, precipitate-free and impurity-free juice which is obtained by manually drilling and collecting at the base of a trunk of the birch between thawing and early spring leaf emergence and has birch faint scent and rich nutrition. The birch juice is commercially available and used as such, for example from greater Khingan over wild berry development, LLC.
The concentrated birch sap of the present invention is obtained by concentrating the above-mentioned commercially available products. Concentration methods are known in the art, such as heat concentration, low temperature vacuum concentration, membrane concentration, and the like. In the present invention, the concentration is preferably performed by a low-temperature freeze concentration or membrane concentration process, for example, commercially available birch juice stock solution is fed into a low-temperature drying device, cooled to-40 ℃ to-70 ℃, and vacuumized to 0.1 to 30Pa to perform low-temperature vacuum concentration, so as to obtain concentrated birch juice with different concentration times.
Unexpectedly, the inventor finds that the concentrated birch juice has significantly better moistening, moisturizing and anti-aging effects compared with the unconcentrated birch juice stock solution, and the concentrated birch juice has the effects of promoting the synthesis of collagen I, III, IV, VII, XVII, elastin, laminin and the like in extracellular matrix, reducing the expression of metal matrix protease MMP-1, promoting the generation of hyaluronic acid, strengthening the structural integrity and functionality of epidermis, dermis and dermis-epidermis junction, improving the skin elasticity, reducing wrinkles, simultaneously has the effects of improving skin barrier, reducing the loss of percutaneous water, resisting inflammation, relieving the stress and the like.
, the inventor also found that the anti-aging effect of the concentrated birch juice is not simply linear with its concentration, but it shows a trend of increasing and decreasing with the increase of the concentration factor, i.e. when the concentration factor of the birch juice reaches , the concentrated birch juice will inhibit the proliferation and activity of cells to a certain extent of and inhibit the expression of genes related to aging inside and outside the cells.
in another aspect, the invention relates to the use of the concentrated birch sap in anti-aging cosmetic compositions.
In a further aspect, the present invention also relates to anti-aging cosmetic compositions comprising (a) the concentrated birch sap.
In a preferred embodiment, the anti-aging cosmetic composition does not contain a chelating agent such as EDTA salt, sodium polyphosphate, sodium metaphosphate, gluconic acid, and the like.
The anti-aging cosmetic composition does not contain any added water, but does not exclude moisture inherently contained in the components.
The concentrated birch sap is present in an amount of about 18-98% by weight, preferably 20-95% by weight, more preferably 22-90% by weight, most preferably 30-90% by weight, based on the total weight of the anti-aging cosmetic composition.
In addition to the concentrated birch juice of the component (a), the anti-aging cosmetic composition may optionally further comprise (B) ingredients commonly used in skin care cosmetic compositions, including vehicles, active ingredients, and adjuvants, etc. Component (B) is known in the art and can be selected by those skilled in the art as desired, for example, in an amount of about 2 to 82% by weight based on the total weight of the antiaging cosmetic composition.
The vehicle includes, for example, diluents, dispersants or carriers and the like, examples of which include, but are not limited to, ethanol, dipropylene glycol, butylene glycol, and the like. The amount of said vehicle in said cosmetic composition is known in the art, and is for example generally comprised between 0.5 and 20% of the total weight of component (B).
Such actives include, for example, emollients, humectants, anti-aging actives and the like.
Examples of such emollients include, but are not limited to, olive oil, macadamia nut oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, meadowfoam seed oil, safflower seed oil, rosa canina oil, argan oil, jojoba seed oil, sunflower seed oil, palm kernel oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutene, isohexadecane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosinate, isononyl isononanoate, hydrogenated polydecene, tris (ethylhexanoate), cetyl ethylhexanoate, bis-diethoxydiol cyclohexane 1, 4-dicarboxylate, caprylic/capric triglyceride, oleyl erucate myristate, octyldodecanol, dimethicone, octylmethicone, cetyl dimethicone, cyclopentadecyl dimethicone, and the like, examples of solid emollients include, but are not limited to, stearyl alcohol, cetyl stearyl alcohol, shark alcohol, behenyl wax, and the like, these waxes are commonly known in the art, such compositions of these waxes are included in the amounts of these waxes are 50% by weight of these waxes, such as the cosmetic compositions.
Examples of such moisturizers include, but are not limited to, or more of glycerin, diglycerin, butylene glycol, propylene glycol, 1, 3-propanediol, dipropylene glycol, 1, 2-pentanediol, polyethylene glycol-8, polyethylene glycol-32, methyl gluceth-10, methyl gluceth-20, PEG/PPG-17/6 copolymer, glyceryl polyether-7, glyceryl polyether-26, glyceryl glucoside, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, PEG/PPG/polytetramethylene glycol-8/5/3 glycerin, sucrose, trehalose, rhamnose, mannose, raffinose, betaine, erythritol, xylitol, urea, glyceryl polyether-5 lactate, sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, sodium polyglutamate, hydrolyzed sclerotium rolum, pullulan, tremella polysaccharides, tamarind seed polysaccharides, and the like.
Examples of such anti-aging active ingredients include, but are not limited to, tocopherol (vitamin E), retinol palmitate, hydrolyzed collagen, hydrolyzed elastin, allantoin, yeast extract, oryzanol, tetrahydrocurcumin, ellagic acid, ubiquinone, whey protein, acetyl hexapeptide-8, palmitoyl pentapeptide-4, salicylphytosphingosine, condensed birch juice, silymarin, sericin, sodium tocopheryl phosphate, ribonucleic acid (RNA), dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, oligopeptide-1, hexapeptide-9, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, grape (VITIS VINIFERA) seed extract, wood flower (pterus MARSUPIUM) bark extract, tea (CAMELLIA SINENSIS) polyphenol, wine extract, apple seed extract, cyclobalanopsis (falva SYLVATICA) bud extract, hydrolyzed sinomenia (eriodigita) extract, hydrolyzed extract, phytolacca acetylated rhaponticum extract, PTEROCARPUS officinalis (eriodictyota) extract, lactobacillus helophora (eriodictyol) extract, lactobacillus plantarum, lactobacillus bifidus (acacia) extract, lactobacillus plantarum, lactobacillus plantarum-0.01, lactobacillus plantarum, lactobacillus plantarum-2, lactobacillus plantarum, lactobacillus plantarum, lactobacillus, etc., among the like, and so forth in the like.
Such adjuvants include, for example, emulsifiers, thickeners, preservatives, fragrances and the like.
Examples of such emulsifiers include, but are not limited to, cetearyl olivate, sorbitan olivate, polysorbate-60, polysorbate-80, methylgluco-sesquistearate, PEG-20 methylgluco-sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-butanoeth-26, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, steareth-2, steareth-21, PPG-13-decyltetraeth-24, cetearyl glucoside, PEG-100 stearate, glyceryl stearate SE, coco glucoside, ceteareth-25, PEG-40 stearate, polyglyceryl-3 methylgluco distearate, glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose % of the total weight of such emulsifiers, e.g. the emulsifier is generally known in the art, and the amount of such emulsifiers are 0.5% by weight of the cosmetic composition.
Examples of the thickener include, but are not limited to, kinds or more of high molecular polymers such as carbomers, acrylates and derivatives thereof, xanthan gum, arabic gum, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, etc. the content of the thickener in the cosmetic composition is known in the art, and for example, it is usually 0.1 to 10% by weight based on the total weight of the component (B).
Examples of such preservatives include, but are not limited to, or more of methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenylethyl alcohol, bis (hydroxymethyl) imidazolidinyl urea, potassium sorbate, sodium benzoate, chlorphenesin, sodium dehydroacetate, caprylhydroxamic acid, 1, 2-hexanediol, 1, 2-pentanediol, p-hydroxyacetophenone, capryl glycol, caprylic glyceride, glyceryl deca carbonate, sorbitan caprylate, ethylhexylglycerin, moutan root extract, and the like, the content of such preservatives in the cosmetic composition is known in the art, for example, it is typically 0.01 to 2% by weight of the total component (B).
The anti-aging cosmetic composition of the present invention may be prepared by any suitable method known in the art. For example, it is prepared using a dissolving tank, an emulsifying pot, a disperser, a transfer pump, etc., which are commonly used in the cosmetic field. The preparation method comprises putting water soluble substance into water phase dissolving kettle, putting oil soluble substance into oil phase dissolving kettle, heating the two kettles to about 80 deg.C, wherein the raw material easy to agglomerate can be pre-dispersed with disperser. After the dissolution is finished, the oil phase and the water phase are conveyed into an emulsifying pot, and homogenized and emulsified for about 5-15 minutes. After emulsification is finished, the temperature of the material body is reduced to normal temperature, optional essence, preservative and the like are added, and the pH of the product is adjusted according to needs. After the relevant detection indexes are qualified, the products can be filled and delivered.
The preparation method can be deleted or adjusted according to the requirements of dosage forms. The anti-aging cosmetic composition can be prepared into various dosage forms such as cream, emulsion, essence and the like according to needs.
Examples
The invention is described in further detail at with reference to the following examples, however, it should be understood that these examples and comparative examples are intended only to illustrate the invention in more detail and should not be construed as limiting in any way the scope of the appended claims.
Example 1: effect of stock solution and concentrated birch sap on aging-related gene expression
In this example, the effect of birch sap stock and concentrated birch sap at different concentration folds on senescence-associated gene expression was tested and compared.
1. Concentration of birch sap
Fresh birch juice stock solution purchased from Daxingan Ling surpassing the company Limited for wild berry development is input into a low-temperature drying device, cooled to-65 ℃, vacuumized to 0.1Pa, and concentrated to 1.05 times, 1.1 times, 1.2 times, 1.5 times, 2 times, 4 times and 8 times respectively.
2. Testing
An experimental instrument: a fluorescent quantitative PCR instrument (Roche), a super clean bench (Sujing), a carbon dioxide incubator (Binder), a microplate reader (BIO-TEK) and a micro oscillator.
Experimental reagents and consumables: human primary fibroblasts, a 6-well plate, fibroblast culture solution, an RNA extraction kit, a reverse transcription kit, Trizol lysate and the like.
The fibroblast-based gene expression analysis procedure was as follows:
(1) inoculation: cells were seeded into 6-well plates at a seeding density of 5E 5/well at 37 ℃ and 5% CO2Incubating in an incubator overnight;
(2) administration: when the cell plating rate in the 6-hole plate reaches about 60%, adding the test substances of each group, and arranging 6 multiple holes in each group;
(3) collecting a sample: at 37 ℃ and 5% CO2After 24 hours in the incubator, discarding the culture solution, adding 1mL of Trizol into each hole, blowing and beating the lysed cells, and collecting samples;
(4) and (3) PCR detection: extracting RNA, carrying out reverse transcription to cDNA, and carrying out fluorescent quantitative PCR detection;
(5) and (3) analysis: by using 2-△△CTThe method carries out result calculation and adopts a T-Test method for statistical analysis.
The test results are shown in the following table.
Note: indicates significant compared to the stock solution, P value less than 0.05; indicates very significant compared to the stock solution,
the P value is less than 0.01.
The results show that the birch juice with the concentration multiple of 1.1-4 times obviously improves the expression quantity of the aging related genes of the basal fibroblast compared with the birch juice stock solution, and particularly, when the concentration multiple is 1.2-2 times, the gene expression quantities of collagen I, collagen III, collagen IV, collagen VII, elastin and laminin are all obviously higher than that of the birch juice stock solution.
Example 2: effect of stock birch juice and concentrated birch juice on aging-related protein expression
In this example, the effect of birch sap stock and different fold concentrations of birch sap on senescence-associated protein expression was tested and compared.
1. Concentration of birch sap
Fresh birch juice stock solution purchased from Daxingan Ling surpassing the company Limited for wild berry development is input into a low-temperature drying device, cooled to-65 ℃, vacuumized to 0.1Pa, and concentrated to 1.05 times, 1.1 times, 1.2 times, 1.5 times, 2 times, 4 times and 8 times respectively.
2. Testing
An experimental instrument: super clean bench (Sujing), plate washing machine (BIO-RAD), enzyme labeling instrument (BIO-TEK), carbon dioxide incubator (Binder)
Experimental reagents and consumables: human primary fibroblasts, a 12-hole plate, fibroblast culture solution, ELISA detection kits with different indexes and the like.
The test procedure was as follows:
(1) inoculating at the inoculation density of 2E 5/well in 12-well culture plate, culturing at 37 deg.C in 5% CO2 incubator, and changing culture medium every two days for times;
(2) administration: when the cell fusion reaches more than 60 percent again, adding the test substances of different groups, and arranging 6 compound holes in each group;
(3) collecting a sample: at 37 ℃ and 5% CO2After 48 hours in the incubator, discarding the culture solution, adding 1mL of Trizol into each hole, blowing and beating the lysed cells, and collecting the samples;
(4) and (3) detection: performing index measurement according to a measurement method of the ELISA kit;
(5) and (3) analysis: and carrying out statistical analysis by adopting a T-Test method.
The test results are shown in the following table.
| Sample (I) | Collagen I (ng) | Collagen III (ng) | Collagen IV (ng) | Collagen VII (ng) | Elastin (ng) | Laminin (ng) |
| Stock solution of birch juice | 118.209±9.242 | 111.745±8.861 | 15.048±1.297 | 11.215±1.431 | 2.892±0.217 | 1.286±0.161 |
| Concentrating by 1.05 times | 121.126±9.817 | 114.236±9.250 | 15.721±1.332 | 12.736±1.038 | 3.011±0.319 | 1.317±0.141 |
| Concentrating by 1.1 times | 148.105±8.275* | 129.434±6.117* | 19.156±1.676* | 18.245±1.993** | 4.833±0.473** | 2.518±0.222** |
| Concentrating by 1.2 times | 176.156±9.239** | 159.023±10.781** | 21.312±2.093** | 18.016±2.082** | 5.022±0.515** | 2.566±0.248** |
| Concentrating by 1.5 times | 168.007±10.641** | 151.279±10.391** | 20.298±2.163** | 16.719±1.478** | 4.913±0.483** | 2.609±0.216** |
| Concentrating by 2 times | 156.119±10.393** | 143.209±7.191** | 19.886±0.979** | 17.914±1.838** | 4.706±0.412** | 2.892±0.237** |
| Concentrating by 4 times | 137.263±7.088* | 131.783±8.231* | 18.716±1.751* | 16.413±1.359* | 4.318±0.392** | 2.288±0.216** |
| Concentrating by 8 times | 127.135±9.943 | 113.135±9.545 | 15.643±1.581 | 11.617±1.011 | 2.984±0.218 | 1.253±0.117 |
Note: indicates significant compared to the stock solution, P value less than 0.05; indicates very significant compared to the stock solution,
the P value is less than 0.01.
The results show that the birch juice with the concentration multiple of 1.1-4 times can obviously improve the expression quantity of the basement fibroblast and the protein related to aging compared with the birch juice stock solution, and particularly, when the concentration multiple is 1.2-2 times, the protein expression quantities of collagen I, collagen III, collagen IV, collagen VII, elastin and laminin are all obviously higher than that of the birch juice stock solution.
Example 3: effect of stock and concentrated birch juice on tissue (reconstituted 3D full-thickness skin model)
In this example, the effect of birch juice stock and different fold concentrations of birch juice on a recombinant 3D full-thickness skin model was tested and compared.
1. Concentration of birch sap
Fresh birch juice stock solution purchased from Daxingan mountain surpassing the company Limited for wild berry development is input into a reverse osmosis circulating device, the operating pressure is controlled at 0.5-5bar, the operating temperature is controlled at 20-35 ℃, and the circulation is carried out until the birch juice is respectively concentrated to 1.1 times, 1.2 times, 1.5 times, 2 times and 4 times.
2. Test method
An experimental instrument: clean bench (Sujing), carbon dioxide incubator (Binder), UVA and UVB irradiators, microplate reader (BIO-TEK), and cryomicrotome (Leica).
Experimental reagents and consumables: a 3D full-thickness skin model (self-made in a laboratory), ELISA detection kits with different indexes, MTT (3- (4, 5-dimethylthiazole-2) -2, 5-diphenyl tetrazole bromide) kits and the like.
The test procedure was as follows:
(1)3D full-layer model construction: constructing a 3D skin model by using keratinocytes and fibroblasts;
(2) molding and administration, namely performing SSUV irradiation treatment (UVA: 30J/cm 2; UVB: 50mJ/cm2) every day when the model leaves a factory, namely 0 day, then respectively coating quantitative samples on the surfaces of corresponding models, coating only model culture solution on a model control group, coating times every day for 6 multiple holes of each group, and keeping the total action time for 4 days;
(3) collecting and detecting: after the action of the sample is finished, collecting culture solution, using an ELISA kit to measure MMP-1 (matrix metalloproteinase 1), taking 3 models from each group, using an MTT kit to test cell proliferation activity to obtain an MTT value, performing tissue fixation on the rest 3 models, and measuring the content of collagen IV at the true epidermal junction by IHC staining.
(4) And (3) analysis: and carrying out statistical analysis by adopting a T-Test method.
The test results are shown in the following table.
Note: indicates significant compared to the stock solution, P value less than 0.05; indicates very significant compared to the stock solution,
the P value is less than 0.01.
MTT value test results show that compared with the birch juice stock solution, the birch juice with the concentration multiple of 1.1-4 times can obviously improve the tissue activity of the recombinant 3D full-thickness skin model. Similarly, the relative expression level of collagen IV at the epidermal junction of the 3D skin model using birch juice concentrated by 1.1-4 times is significantly higher than that of the birch juice stock solution. The MMP-1 result shows that the concentration of the birch juice of 1.1 to 4 times is obviously lower than that of the birch juice stock solution. The results also show that when the concentration multiple of the birch juice is 1.1-2, the tissue activity of the recombinant 3D full-thickness skin model can be remarkably improved, the relative expression quantity of collagen IV at the junction of the true epidermis is promoted, and meanwhile, the expression of MMP-1 in the extracellular matrix can be reduced.
Example 4: preparation of anti-aging cream composition
The formula of the anti-aging cream composition is shown in the following table:
the anti-aging cream composition is prepared as follows:
1. oil phase: adding the No. 4, 6, 7, 8, 9, 11, 12, 13, 15 and 21 raw materials into an oil phase pot, heating to 80 ℃, dissolving and uniformly mixing;
2. mixing No. 2, No. 16 and No. 18 raw materials at normal temperature;
3. mixing No. 10, No. 14, No. 17, No. 20 raw materials at room temperature;
4. water phase: heating the raw materials No. 1,3 and 5 to 80 ℃, adding the mixture obtained in the step 2, dissolving and uniformly mixing;
5. emulsification: adding the water phase and the oil phase into an emulsification tank, keeping the temperature at 80 ℃, homogenizing and emulsifying at the speed of 3000rpm for 5 minutes, and adding the No. 19 raw material after emulsification;
6. and (3) adding the mixture obtained in the step (3) when the temperature is reduced to 40 ℃ by stirring, uniformly stirring, and discharging to obtain the anti-aging cream composition.
In this example, the effect of the anti-aging cream composition described above on a recombinant 3D full-thickness skin model was tested.
As a result, the anti-aging cream composition can remarkably improve the tissue vitality of a recombinant 3D full-thickness skin model, promote the relative expression quantity of collagen IV at the real epidermal junction, and simultaneously reduce the expression of MMP-1 in extracellular matrix.
Example 5: preparation of anti-aging eye cream composition
The formula of the anti-aging eye cream composition is shown in the following table:
| serial number | Raw materials | By weight% |
| 1 | 2-fold concentrated birch (Betula ALBA) sap | 70.75 |
| 2 | Glycerol | 6.00 |
| 3 | Glycerol tri (ethylhexanoate) ester | 3.00 |
| 4 | Butanediol | 5.00 |
| 5 | Polydimethylsiloxane | 2.00 |
| 6 | C12-20 alkyl glucoside, C14-22 alcohol | 2.00 |
| 7 | Cetyl alcohol | 2.00 |
| 8 | Creatine | 2.00 |
| 9 | Phenyl trimethicones | 1.00 |
| 10 | Hexapeptide-9 | 3.00 |
| 11 | Glyceryl stearate, PEG-100 stearate | 0.50 |
| 12 | Phenoxyethanol | 0.50 |
| 13 | Panthenol | 0.30 |
| 14 | Dipeptide Diaminobutyrylbenzylamide diacetate | 1.00 |
| 15 | Arginine | 0.15 |
| 16 | Carbomer | 0.25 |
| 17 | Acetylated sodium hyaluronate | 0.10 |
| 18 | Hydroxy phenyl methyl ester | 0.10 |
| 19 | Allantoin | 0.15 |
| 20 | Polyglutamic acid sodium salt | 0.20 |
The anti-aging eye cream composition is prepared as follows:
1. oil phase: adding No. 3, 5, 6, 7, 9 and 11 raw materials into an oil phase pot, heating to 80 ℃, dissolving, and uniformly mixing;
2. uniformly mixing No. 2 and No. 16 raw materials at normal temperature;
3. uniformly mixing the raw materials such as No. 10, No. 14 and No. 17 raw materials at normal temperature;
4. water phase: heating the No. 1,4, 8, 13, 18, 19 and 20 raw materials to 80 ℃, adding the mixture obtained in the step 2, dissolving and uniformly mixing;
5. emulsification: adding the water phase and the oil phase into an emulsification tank, keeping the temperature at 80 ℃, homogenizing and emulsifying at the speed of 3000rpm for 5 minutes, and adding the No. 12 and No. 15 raw materials after emulsification;
6. and (3) adding the mixture obtained in the step (3) when the temperature is reduced to 40 ℃ by stirring, uniformly stirring, and discharging to obtain the anti-aging eye cream composition.
Using the half-face control test method, 20 volunteers were tested before and 4 weeks after using the product as follows:
1) using primos to photograph the external canthus on the left and right sides of the volunteer, and using software to calculate wrinkle parameters, wherein the wrinkle parameters comprise the number of wrinkles, the area of the wrinkles, the depth of the wrinkles and the like; and
2) the moisture content of the skin was measured on the left and right canthi using a Corneometer.
The results showed a significant increase in skin moisture content in the corner of the eye in 18 out of 20 subjects, with 15 of the eye's corner wrinkles being significantly lighter and lighter, the wrinkle area being reduced, and the number of wrinkles being reduced.
Example 6: anti-aging emulsion composition
The formula of the anti-aging emulsion composition is shown in the following table:
| raw materials | By weight% | |
| 1 | 1.1 times of concentrated succus Betulae Pendulae (Betula ALBA) | 67.55 |
| 2 | Glycerol | 6.00 |
| 3 | Dipropylene glycol | 5.00 |
| 4 | Squalane | 5.00 |
| 5 | Butyrospermum PARKII (Butyrum PARKII) fruit fat | 2.00 |
| 6 | Synthetic spermaceti | 1.00 |
| 7 | Isododecane | 2.00 |
| 8 | Xanthan gum | 0.10 |
| 9 | Acetyl hexapeptide-8 | 8.50 |
| 10 | Acetylated sodium hyaluronate | 0.05 |
| 11 | Acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer | 0.10 |
| 12 | PEG-100 stearate, glyceryl stearate | 1.00 |
| 13 | C14-22 alcohol, C12-20 alkyl glucoside | 1.00 |
| 14 | Hydroxy phenyl methyl ester | 0.20 |
| 15 | Arginine | 0.10 |
| 16 | Phenoxyethanol | 0.40 |
The anti-aging emulsion composition is prepared as follows:
1. oil phase: adding the No. 4, 5, 6, 7, 12 and 13 raw materials into an oil phase pot, heating to 80 ℃, dissolving and uniformly mixing;
2. uniformly mixing the No. 2, No. 8 and No. 11 raw materials at normal temperature;
3. uniformly mixing the raw materials such as No. 9, No. 10 and No. 16 raw materials at normal temperature;
4. water phase: heating the No. 1,3 and 14 raw materials to 80 ℃, adding the mixture obtained in the step 2, dissolving and uniformly mixing;
5. emulsification: adding the water phase and the oil phase into an emulsification tank, keeping the temperature at 80 ℃, homogenizing and emulsifying at the speed of 3000rpm for 5 minutes, and adding the No. 15 raw material after emulsification;
6. and (3) adding the mixture obtained in the step (3) when the temperature is reduced to 40 ℃ by stirring, uniformly stirring, and discharging to obtain the anti-aging emulsion composition.
After 4 weeks of using the above formula emulsion for 20 subjects, the use was subjectively evaluated. Results 18 people reflected a significant increase in skin moisture content, with smooth, tender, and elastic skin. Of these, 16 reflected a significant improvement in the lines of stature and canthus.
Example 7: anti-aging essence composition
The formula of the anti-aging essence composition is shown in the following table:
the anti-aging essence composition is prepared as follows:
1. adding the raw materials No. 1,2, 3, 4, 6, 12 and 13 into an emulsification tank, and keeping the temperature at 80 ℃;
2. mixing No. 5, No. 10 and No. 11 raw materials at normal temperature;
3. uniformly mixing the No. 7, 8 and 9 raw materials at normal temperature;
4. adding No. 14 raw materials, and adjusting the pH value;
5. stirring and cooling to 40 ℃, adding the raw materials No. 15, No. 16 and the step 3, uniformly stirring and discharging to obtain the anti-aging essence composition.
Using the half-face control test method, 20 volunteers were tested before and 8 weeks after using the product as follows:
1) using primos to photograph the external canthus on the left and right sides of the volunteer, and using software to calculate wrinkle parameters, wherein the wrinkle parameters comprise the number of wrinkles, the area of the wrinkles, the depth of the wrinkles and the like;
2) testing the moisture content of the skin of the left and right canthus by using a Corneometer; and
3) the left and right cheek skin water loss rate (TEWL value) was measured with a Tewameter.
The results showed a significant increase in skin moisture content in the corner of the eye in 18 out of 20 subjects, with 15 of the eye's corner wrinkles being significantly lighter and lighter, the wrinkle area being reduced, and the number of wrinkles being reduced. Of the 20 subjects, 18 had a significant increase in human skin moisture and a significant decrease in TEWL values, with 16 of the facial lines and canthus wrinkles being significantly lighter and lighter, the wrinkle area being reduced, and the number of wrinkles being reduced.
The technical solutions of the above-described embodiments are preferred embodiments of the present invention, and several modifications and changes can be made without departing from the principle of the present invention, and these modifications and changes should also be considered as being within the protection scope of the present invention.
Claims (6)
- concentrated birch juice with concentration ratio of 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
- 2. Use of concentrated birch sap in an anti-aging cosmetic composition, wherein the concentrated birch sap is concentrated 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
- 3. The use of claim 2, wherein the anti-aging cosmetic composition does not comprise any added water.
- an anti-aging cosmetic composition comprising concentrated birch sap, wherein the concentrated birch sap is concentrated 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times.
- 5. The anti-aging cosmetic composition of claim 4, comprising 18 to 98% by weight, preferably 20 to 95% by weight, more preferably 22 to 90% by weight, most preferably 30 to 90% by weight of concentrated birch juice, based on the total weight of the anti-aging cosmetic composition.
- 6. The anti-aging cosmetic composition of claim 4 or 5, wherein the anti-aging cosmetic composition does not comprise any added water.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910694725.8A CN110731928A (en) | 2019-07-30 | 2019-07-30 | Anti-aging cosmetic composition |
| JP2020055582A JP7329474B2 (en) | 2019-07-30 | 2020-03-26 | Anti-aging cosmetic composition |
| PCT/CN2020/102177 WO2021017833A1 (en) | 2019-07-30 | 2020-07-15 | Anti-aging cosmetic composition |
| JP2021212805A JP2022059604A (en) | 2019-07-30 | 2021-12-27 | Anti-aging cosmetic composition |
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| CN201910694725.8A CN110731928A (en) | 2019-07-30 | 2019-07-30 | Anti-aging cosmetic composition |
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| CN110731928A true CN110731928A (en) | 2020-01-31 |
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| JP (2) | JP7329474B2 (en) |
| CN (1) | CN110731928A (en) |
| WO (1) | WO2021017833A1 (en) |
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| WO2021017833A1 (en) * | 2019-07-30 | 2021-02-04 | 浙江养生堂天然药物研究所有限公司 | Anti-aging cosmetic composition |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007320921A (en) * | 2006-06-01 | 2007-12-13 | Nitta Gelatin Inc | Cosmetic comprising sap formulated therein |
| CN105662940A (en) * | 2016-01-05 | 2016-06-15 | 深圳典立生物科技有限公司 | Active composition, and preparation method and application thereof in cosmetics or skin care products |
| CN108186482A (en) * | 2018-03-31 | 2018-06-22 | 佛山文森特知识产权服务有限公司 | A kind of tender white skin care compositions of water and its application |
| CN108186527A (en) * | 2018-02-28 | 2018-06-22 | 程苗 | A kind of cosmetic skin-care composition |
| CN109044929A (en) * | 2018-09-14 | 2018-12-21 | 江苏魅莉萱言生物科技有限公司 | A kind of surfactant with moisturizing, anti-aging and whitening spot-removing function |
| CN109077960A (en) * | 2018-09-13 | 2018-12-25 | 创领化妆品科技（北京）有限公司 | A kind of anti-ageing lightening compositions of white birch myron |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001058920A (en) * | 1999-08-20 | 2001-03-06 | Kao Corp | Cosmetic |
| JP2006131546A (en) * | 2004-11-05 | 2006-05-25 | Asahi Kasei Life & Living Corp | Bath agent containing tree sap of japanese white birch |
| JP2008088109A (en) * | 2006-10-02 | 2008-04-17 | Nitta Gelatin Inc | White birch extract composition and cosmetic comprising white birch formulated therein |
| CN107049865B (en) * | 2017-04-27 | 2018-03-27 | 养生堂（上海）化妆品研发有限公司 | Skin care cosmetic compositions |
| CN107019660B (en) * | 2017-04-27 | 2018-05-11 | 养生堂（上海）化妆品研发有限公司 | Moisture-keeping cosmetics composition |
| CN110731928A (en) * | 2019-07-30 | 2020-01-31 | 浙江养生堂天然药物研究所有限公司 | Anti-aging cosmetic composition |
| CN110731924B (en) * | 2019-07-30 | 2022-05-17 | 浙江养生堂天然药物研究所有限公司 | Enhanced anti-aging cosmetic compositions |
-
2019
- 2019-07-30 CN CN201910694725.8A patent/CN110731928A/en active Pending
-
2020
- 2020-03-26 JP JP2020055582A patent/JP7329474B2/en active Active
- 2020-07-15 WO PCT/CN2020/102177 patent/WO2021017833A1/en active Application Filing
-
2021
- 2021-12-27 JP JP2021212805A patent/JP2022059604A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007320921A (en) * | 2006-06-01 | 2007-12-13 | Nitta Gelatin Inc | Cosmetic comprising sap formulated therein |
| CN105662940A (en) * | 2016-01-05 | 2016-06-15 | 深圳典立生物科技有限公司 | Active composition, and preparation method and application thereof in cosmetics or skin care products |
| CN108186527A (en) * | 2018-02-28 | 2018-06-22 | 程苗 | A kind of cosmetic skin-care composition |
| CN108186482A (en) * | 2018-03-31 | 2018-06-22 | 佛山文森特知识产权服务有限公司 | A kind of tender white skin care compositions of water and its application |
| CN109077960A (en) * | 2018-09-13 | 2018-12-25 | 创领化妆品科技（北京）有限公司 | A kind of anti-ageing lightening compositions of white birch myron |
| CN109044929A (en) * | 2018-09-14 | 2018-12-21 | 江苏魅莉萱言生物科技有限公司 | A kind of surfactant with moisturizing, anti-aging and whitening spot-removing function |
Non-Patent Citations (4)
| Title |
|---|
| 江德森等: ""桦树液浓缩研究"", 《吉林林学院学报》 * |
| 盛艳等: ""桦树汁营养成分及功能利用研究进展"", 《农产品加工》 * |
| 蒋中海: ""黑龙江桦树汁的研究"", 《食品工业科技》 * |
| 黑龙江省卫生健康委员会: ""食品安全地方标准白桦树汁浓缩液"", 《黑龙江省地方标准》 * |
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|---|---|---|---|---|
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| CN111329810A (en) * | 2020-04-10 | 2020-06-26 | 上海新高姿化妆品有限公司 | Antioxidant anti-aging cosmetic composition |
| CN111329810B (en) * | 2020-04-10 | 2022-10-18 | 上海新高姿化妆品有限公司 | Antioxidant anti-aging cosmetic composition |
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Also Published As
| Publication number | Publication date |
|---|---|
| JP2022059604A (en) | 2022-04-13 |
| JP2021020886A (en) | 2021-02-18 |
| JP7329474B2 (en) | 2023-08-18 |
| WO2021017833A1 (en) | 2021-02-04 |
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